Magnetic porous reaction plate

Abstract

The utility model discloses a magnetic porous reaction plate, relates to a biological medicine research and development device, and is used for life science experiment research using magnetic beads. The reaction plate comprises a perforated plate, a base, a cover plate and a magnet, and the magnet can be embedded between the perforated plate and the base and is matched with the bottom of the perforated plate in shape. According to the utility model, the porous reaction plate can be moved, centrifuged, shaken and the like under the condition of keeping magnetic force, so that more flexible and diversified experiment designs and experiment schemes are realized.

Description

Technical Field

[0001] This utility model relates to a biomedical research and development device, and more specifically, to a magnetic porous reaction plate. Background Technology

[0002] Magnetic bead technology has wide applications in the life sciences (biology, basic medicine, pharmacy, etc.), especially in the separation, enrichment, purification and detection of biomolecules.

[0003] Magnetic bead technology can be used to separate and enrich biomolecules such as DNA (deoxyribonucleic acid), RNA (ribonucleic acid), proteins, and cells. By combining specific targets with surface-functionalized magnetic beads, the target molecules can be selectively enriched, while the target molecules can be easily separated and recovered by applying an external magnetic field.

[0004] Magnetic bead technology can be used for efficient purification of DNA and RNA: specific ligands or affinity modifiers on the surface of magnetic beads can specifically bind to target DNA / RNA fragments, and non-specific bindings can be removed by a washing step, thereby achieving high-purity DNA / RNA purification.

[0005] Magnetic bead technology can be used for antibody purification and separation: by binding antigens to magnetic beads, specific antibodies can be selectively enriched and purified. Furthermore, magnetic bead technology can also be used in immunoassays, such as ELISA and immunomagnetization assays.

[0006] Magnetic bead technology can be used for the rapid separation and enrichment of cells: antibodies or other affinity molecules on the surface of magnetic beads can bind to specific antigens on the cell surface, thereby achieving selective enrichment of specific cell subpopulations. Furthermore, magnetic bead technology can also be used for cell labeling and tracking; by labeling magnetic beads with specific functions or properties, cell localization and monitoring can be achieved.

[0007] In high-throughput experiments, magnetic bead technology often uses a porous reaction plate in conjunction with a magnetic frame to separate the magnetic beads. Once the porous plate is removed from the magnetic frame, the magnetic force on the beads within the pores disappears. Summary of the Invention

[0008] This invention provides a magnetic porous reaction plate for life science experimental research using magnetic beads, which enables the movement, centrifugation, and shaking of the porous reaction plate while maintaining magnetic force.

[0009] The technical solution of this utility model is as follows:

[0010] A magnetic porous reaction plate includes a porous plate 1, a base 2, a cover plate 3, and a magnet 4. The porous plate 1 can be fitted into the base 2, and the cover plate 3 can be tightly covered on the base 2.

[0011] The magnet 4 can be embedded between the multi-well plate 1 and the base 2 and fits the shape of the bottom of the multi-well plate.

[0012] The cover plate 3 has a sealing gasket 5 inside.

[0013] The multi-well plate 1, the base 2, and the cover plate 3 are made of a strong, corrosion-resistant, and transparent plastic, such as polystyrene or polypropylene.

[0014] The magnet 4 is made of a neodymium iron boron (NdFeB) magnet.

[0015] The sealing gasket 5 is made of rubber, silicone, or plastic.

[0016] One use method of the present application is to sequentially add a reaction solution into the multi-well plate 1, embed the magnet 4 in the base 2, then embed the multi-well plate 1 containing the solution on the magnet 4 in the base 2, cover the cover plate 3, as shown in Figure 2 , and perform moving, centrifuging, shaking, and other experimental operations.

[0017] The present application can also be used without a magnet: sequentially add a reaction solution into the multi-well plate 1, then embed the multi-well plate 1 containing the solution in the base 2, cover the cover plate 3, as shown in Figure 3 , and perform moving, centrifuging, shaking, and other experimental operations.

[0018] The present application has the beneficial effect of enabling moving, centrifuging, shaking, and other operations on the multi-well reaction plate while maintaining the magnetic force, thereby enabling more flexible and diverse experimental design and experimental schemes. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 : A longitudinal sectional view of a magnetic multi-well reaction plate before assembly.

[0020] Figure 2 : A longitudinal sectional view of a magnetic multi-well reaction plate after assembly with a magnetic module.

[0021] Figure 3 : A longitudinal sectional view of a magnetic multi-well reaction plate after assembly without a magnetic module.

[0022] REFERENCE NUMERALS: 1 - multi-well plate, 2 - base, 3 - cover plate, 4 - magnet, 5 - sealing gasket. DETAILED DESCRIPTION

[0023] Example 1, Structure of a Magnetic Multi-well Reaction Plate

[0024] A magnetic porous reaction plate includes a porous plate 1, a base 2, a cover plate 3, and a magnet 4. The porous plate 1 can be fitted into the base 2, and the cover plate 3 can be tightly covered on the base 2.

[0025] The magnet 4 can be fitted between the perforated plate 1 and the base 2, and fits the shape of the bottom of the perforated plate.

[0026] The cover plate 3 has a sealing gasket 5 inside.

[0027] The porous plate 1, base 2, and cover plate 3 are made of sturdy, corrosion-resistant, and transparent polystyrene plastic.

[0028] The magnet 4 is made of neodymium iron boron magnet.

[0029] The sealing gasket 5 is made of silicone.

[0030] Example 2: How to use a magnetic porous reaction plate

[0031] One method of using this invention is as follows: The reaction solution is sequentially added into the porous plate 1; the magnet 4 is fitted into the base 2; then the porous plate 1 containing the solution is fitted onto the magnet 4 within the base 2; and the cover plate 3 is then placed on top. Figure 2 As shown, perform experimental operations such as moving, centrifuging, and shaking.

[0032] This invention can also be used without a magnet: the reaction solution is added sequentially into the porous plate 1, then the porous plate 1 containing the solution is fitted into the base 2, and the cover plate 3 is placed on top. Figure 3 As shown, perform experimental operations such as moving, centrifuging, and shaking.

Claims

1. A magnetically porous reaction plate, characterized in that, It includes a perforated plate (1), a base (2), a cover plate (3) and a magnet (4). The perforated plate (1) can be fitted into the base (2), the cover plate (3) can be tightly covered on the base (2), and the magnet (4) can be fitted between the perforated plate (1) and the base (2) and fits the bottom shape of the perforated plate.

2. The magnetic porous reaction plate according to claim 1, characterized in that, The cover plate (3) has a sealing gasket (5) inside.

3. The magnetic porous reaction plate according to claim 1, characterized in that, The porous plate (1), base (2), and cover plate (3) are made of polystyrene or polypropylene.

4. The magnetic porous reaction plate according to claim 1, characterized in that, The magnet (4) is made of neodymium iron boron magnet.

5. The magnetic porous reaction plate according to claim 2, characterized in that, The sealing gasket (5) is made of rubber, silicone or plastic.

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