Slide glass, slide glass device and fluorescence detection system

Abstract

The utility model provides a slide glass, the whole slide glass is in a sheet shape, the slide glass comprises a main body layer and a reflective layer laminated on one surface of the main body layer, and the reflective layer is used for reflecting light incident to the slide glass and reflecting fluorescence emitted by a cell or tissue sample. The utility model also provides a slide glass device comprising the slide glass and a fluorescence detection system comprising the slide glass device. The light reflecting layer is arranged to reflect the exciting light, and the reflected exciting light can perform secondary excitation on the sample, so that the excitation effect is improved under the condition of not increasing the power of the excitation light source, and the intensity of an emitted light signal can also be improved. In addition, the reflective layer is light-proof, so that light rays do not penetrate through the slide glass and then irradiate the objective table at the bottom to generate reflection light, and the adaptability to a microscopic imaging platform of a specific model is good.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of fluorescent staining microscopy, in particular to a slide, a slide device comprising the slide and a fluorescent detection system using the slide device. BACKGROUND

[0002] Fluorescent microscopy is an important technical means in contemporary life science research, and plays an important role in the fields of basic scientific research, medical diagnosis and pharmaceutical production. In fluorescent microscopy, fluorescent dyes or antibodies with fluorescent groups are usually used to label samples, and then a specific wavelength of excitation light is used to excite the fluorescent groups to emit fluorescent signals, and the corresponding results can be obtained by observing and measuring the emitted light. However, in actual operation, due to the low content of target molecules to be detected in the sample and / or the suboptimal titer of the antibody used for detection, the fluorescent signal may be weak or even cannot be effectively detected. Therefore, it is a common demand to improve the intensity of the target fluorescent light in fluorescent microscopy. Increasing the power of excitation light can increase the intensity of emitted light, but on the one hand, the power of the hardware light source has a fixed upper limit, and on the other hand, using high-power output will accelerate the aging of the light source such as laser, reducing the service life. Using tyramide signal amplification and other means can also increase the signal intensity, but the experimental process required by such methods is more complex, and the reagent cost is higher. SUMMARY

[0003] The first aspect of the present application provides a slide, which is in the form of a thin sheet, comprising:

[0004] a main layer; and

[0005] a light-reflecting layer laminated on one surface of the main layer, the light-reflecting layer being used for reflecting light incident on the slide and reflecting fluorescent light emitted by a cell or tissue sample.

[0006] In at least one embodiment, the thickness of the light-reflecting layer is 150-200 nm.

[0007] In at least one embodiment, the slide further comprises a functional modification layer laminated on the side of the light-reflecting layer away from the main layer, the functional modification layer being used to increase the adhesion of the cell or tissue sample on the slide.

[0008] In at least one embodiment, the thickness of the functional modification layer is less than 100 µm.

[0009] In at least one embodiment, the slide has opposite first and second surfaces, wherein the second surface is the surface of the main layer away from the light-reflecting layer, and the slide is further formed with at least one liquid inlet and at least one liquid outlet, the at least one liquid inlet and the at least one liquid outlet penetrating the first and second surfaces, respectively.

[0010] In at least one embodiment, the slide is a rectangular sheet, and the at least one inlet port and the at least one outlet port are located at opposite sides of the slide, respectively.

[0011] The second aspect of the present application provides a slide device comprising the slide as described above.

[0012] In at least one embodiment, the slide device further comprises a cover glass and a channel layer stacked on the slide, and the channel layer is located between the cover glass and the slide.

[0013] In at least one embodiment, the channel layer is connected to a first surface of the slide, the first surface being a surface of the slide opposite to the surface facing away from the reflective layer of the main body layer, the channel layer being formed with a sample area, at least one inlet channel and at least one outlet channel, the at least one inlet channel being in communication with the at least one inlet port and the sample area, the at least one outlet channel being in communication with the at least one outlet port and the sample area, and the first surface being exposed to the channel layer through the sample area, the at least one inlet channel and the at least one outlet channel.

[0014] The third aspect of the present application provides a fluorescence detection system, comprising:

[0015] The slide device as described above;

[0016] A flow channel device in communication with the at least one inlet port of the slide and the at least one outlet port of the slide, respectively, for transporting reagents towards the at least one outlet port and recovering reagent waste from the at least one outlet port; and

[0017] A fluorescence imaging device for exciting reagents in the sample area of the slide device and receiving fluorescence generated by the reagents under excitation to generate a fluorescence image of the sample.

[0018] The slide of the present application reflects excitation light through the reflective layer, and the reflected excitation light can excite the sample again, thereby increasing the excitation effect without increasing the power of the excitation light source, and also improving the intensity of the emission light signal. In addition, the reflective layer is opaque, so the light will not pass through the slide to shine on the bottom stage and produce reflected light, which is suitable for specific types of microscopic imaging platforms. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 FIG. 1 is a cross-sectional view of a slide according to an embodiment of the present application.

[0020] Figure 2 FIG. 2 is an exploded view of a slide device according to an embodiment of the present application.

[0021] Figure 3 FIG. 3 is a schematic diagram of a fluorescence detection system according to an embodiment of the present application.Figure 2 A schematic diagram of the slide in the slide device.

[0022] Figure 4 A schematic diagram of the slide device. Figure 2 A schematic diagram of the channel layer and the slide.

[0023] Figure 5 A schematic diagram of the slide device. Figure 2 A schematic diagram of the positioning clip and the cover glass assembled together.

[0024] Figure 6 A schematic diagram of the module structure of the fluorescence detection system of the embodiment.

[0025] Figure 7 A schematic diagram of the slide device and the flow channel device. Figure 6 A schematic diagram of the slide device and the flow channel device.

[0026] Figure 8 A magnified view of a local mouse brain section cell nucleus staining on the slide (with a reflective layer of metal chromium) of the embodiment.

[0027] Main element symbol explanation

[0028] Fluorescence detection system 100, slide device 10, slide 11, main body layer 110, reflective layer 111,

[0029] Function modification layer 112, first surface 115, second surface 116, liquid inlet 113, liquid outlet 114, channel layer 12, sample area 121, liquid inlet channel 122, liquid outlet channel 123, cover glass 13, positioning clip 14, mounting opening 141, positioning hole 142, flow channel device 20, reagent storage unit 21, liquid inlet flow path unit 22, liquid outlet flow path unit 23, waste liquid recovery unit 24, negative pressure driving unit 25, fluorescence imaging device 30, sample 200.

[0030] The following specific embodiments will further illustrate the present application in conjunction with the above-mentioned drawings. Specific embodiments

[0031] The embodiment of the present application provides a fluorescence detection system. The fluorescence detection system is used for emitting laser to excite fluorescent dye on a marker in a biological sample, so that the fluorescent dye is excited to generate fluorescence. The fluorescence detection system can collect fluorescence to generate a fluorescence image. The fluorescence image can reflect the distribution structure of the marker in the sample, etc. The fluorescence detection system is, for example, a fluorescence microscope, a multiple immunofluorescence staining dyeing machine, etc. The biological sample is, for example, a tissue, a cell, etc. In the embodiment, the fluorescence detection system is taken as a multiple immunofluorescence staining dyeing machine, and the biological sample is taken as a tissue section sample.

[0032] In this embodiment, different proteins (such as antibodies) and cell nuclei on the tissue slice sample carry different types of fluorescent dyes, and the fluorescence detection system is used to emit laser light to excite the fluorescent dyes on each protein and cell nucleus, so that different types of fluorescent dyes are excited to generate fluorescent light of different wavelengths. The fluorescence detection system is also used to collect the fluorescent light of different wavelengths, generate a fluorescent image of the tissue slice sample, and detect the protein and cell nucleus distribution information of the tissue slice sample according to the fluorescent image.

[0033] The embodiment of the present application provides a slide for a fluorescence detection system. The slide is used to directly place a sample such as cells or tissues. Referring to Figure 1 The slide 11 includes a main body layer 110 and a reflective layer 111 stacked on one surface of the main body layer 110. The slide 11 is in the form of a sheet. The main body layer 110 can be a glass sheet, a plastic sheet, or a silicon wafer. Generally, the main body layer 110 is transparent, but is not limited thereto. The reflective layer 111 is used to reflect light incident thereon, and can achieve a mirror reflection effect. The reflective layer 111 can be made of various materials that can form the reflective layer 111, such as metal materials, and can be a conventional metal chromium or metal silver. In the embodiment of the present application, the reflective layer 111 is a metal chromium layer. In some embodiments, the thickness of the reflective layer 111 is 150-200 nm.

[0034] The reflective layer 111 can be formed on the surface of the main body layer 110 by a conventional coating method, such as physical vapor deposition or chemical vapor deposition. In some embodiments, the reflective layer 111 can be formed on the surface of the main body layer 110 by an evaporation method (one of physical vapor deposition).

[0035] The existing glass slides are basically made of transparent glass and do not have a design to enhance the fluorescent signal. Therefore, when the fluorescent signal is weak, it is difficult to observe or detect the target fluorescent signal. In addition, on some models of fluorescence microscopic imaging platforms, light passes through the transparent glass slide and then irradiates the bottom stage, and the reflected light also causes adverse effects on imaging.

[0036] The present application adds a reflective layer 111 to the main body layer 110 of the slide 11 to reflect the excitation light. The reflected excitation light will excite the sample again, thereby increasing the excitation effect and improving the emission signal intensity without increasing the power of the excitation light source. In addition, the reflective layer 111 is not transparent, so light will not pass through the slide to irradiate the bottom stage, which is suitable for specific models of microscopic imaging platforms.

[0037] Further, as Figure 1As shown, in some embodiments, the slide 11 further comprises a functional modification layer 112 laminated on the side of the reflective layer 111 away from the main body layer 110. The functional modification layer 112 is used to increase the adhesion of the cell or tissue sample on the slide 11, ensuring that the sample will not fall off during subsequent processing and observation.

[0038] In some embodiments, the functional modification layer 112 can be poly-L-lysine (PLL) or polyethyleneimine (PEI), so that the surface of the slide 11 is positively charged and combined with the negatively charged cell membrane or tissue.

[0039] In some embodiments, the functional modification layer 112 can be a natural protein such as gelatin or collagen, thereby enhancing adhesion.

[0040] In some embodiments, the functional modification layer 112 can be aminosilane. Aminosilane can form a covalent bond with the glass surface, and its amino group can be combined with the carboxyl or phosphate group in the cell or tissue.

[0041] In some embodiments, the thickness of the functional modification layer 112 is less than 100 µm.

[0042] The present application improves the conventional slide by forming a reflective layer 111, thereby increasing the intensity of the fluorescent signal in fluorescent microscopic imaging, and reducing the reflection of light from the bottom stage after the light passes through the slide on a specific type of microscopic imaging platform.

[0043] Please refer to Figure 2 The slide device 10 of the embodiments of the present application comprises the slide 11 described above, and further comprises a channel layer 12 and a cover glass 13 laminated on the slide 11. The slide device 10 is used to load a sample 200. Among them, Figure 2 The channel layer 12 only shows the approximate outer contour, and the detailed structure of the channel layer 12 is described in Figure 4 The slide 11, the channel layer 12 and the cover glass 13 are all in the form of a rectangular thin sheet, and the channel layer 12 is sandwiched between the slide 11 and the cover glass 13. In this embodiment, the planar structure of the slide 11, the channel layer 12 and the cover glass 13 is a rectangle of 25×75 mm. In other embodiments, the shape of the slide 11, the channel layer 12 and the cover glass 13 is not limited to a rectangle.

[0044] Please refer to Figure 3The slide 11 has opposite first and second surfaces 115 and 116, wherein the second surface 116 is the surface of the body layer 110 facing away from the reflective layer 111. The first surface 115 is used for containing the sample 200. When the outermost side of the slide 11 is the reflective layer 111, the first surface 115 is the surface of the reflective layer 111 facing away from the body layer 110. When the outermost side of the slide 11 is the functional modification layer 112, the first surface 115 is the surface of the functional modification layer 112 facing away from the body layer 110.

[0045] The channel layer 12 is disposed on the first surface 115. The slide 11 further has four inlet ports 113 and four outlet ports 114. Each inlet port 113 and each outlet port 114 is a through hole penetrating the first and second surfaces 115 and 116. The four inlet ports 113 are disposed at one side edge of the first surface 115, and the four outlet ports 114 are disposed at the other side edge of the first surface 115. That is, the four inlet ports 113 and the four outlet ports 114 are respectively located at opposite side edges of the slide 11.

[0046] In other embodiments of the present application, the number of inlet ports 113 and outlet ports 114 on the slide 11 can be other numbers greater than or equal to 1, and the positions of the inlet ports 113 and outlet ports 114 can be different.

[0047] In the present embodiment, the channel layer 12 is a double-sided adhesive layer. Please refer to Figure 3 , one surface of the channel layer 12 is fixedly attached to the slide 11, and the other opposite surface is used for fixedly attaching to the cover glass 13.

[0048] Please refer to Figure 4 , the channel layer 12 includes a solid part 12A and a hollow pattern 12B surrounded by the solid part. The hollow pattern 12B is a hollow area formed by cutting part of the material of the channel layer 12. The area on the slide 11 corresponding to the hollow pattern 12B (i.e. the projection area of the hollow pattern 12B on the surface of the slide 11) is exposed relative to the channel layer 12, so that when the channel layer 12 is attached to the slide 11 and the cover glass 13, the area on the channel layer 12 where the hollow pattern 12B is formed is enclosed by the slide 11 and the cover glass 13 to form channels communicating each inlet port 113 and each outlet port 114, which can be used for the flow of reagents and reagent waste.

[0049] In the present embodiment, the hollow pattern 12B includes a sample area 121, four inlet channels 122 and four outlet channels 123. The sample area 121 is located in the central area of the channel layer 12, and the four inlet channels 122 and the four outlet channels 123 are respectively located at different sides of the sample area 121 and communicate with the sample area 121.

[0050] The four liquid inlet channels 122 are in one-to-one correspondence with the four liquid inlet ports 113, i.e., each liquid inlet port 113 is exposed to the channel layer 12 through a corresponding liquid inlet channel 122. The four liquid inlet channels 122 are separated from each other, so that each liquid inlet channel 122 is used for the flow of reagents from a corresponding liquid inlet port 113, and the reagents flowing in each liquid inlet channel 122 are prevented from mixing, the extra space is reduced, the flow rate is stabilized, and air bubbles are avoided. The four liquid outlet channels 123 are in one-to-one correspondence with the four liquid outlet ports 114, i.e., each liquid outlet port 114 is exposed to the channel layer 12 through a corresponding liquid outlet channel 123. The four liquid outlet channels 123 are separated from each other, so that each liquid outlet channel 123 is used for introducing a kind of reagent waste liquid into a corresponding liquid outlet port 114, and the reagent waste liquids flowing in each liquid outlet channel 123 are prevented from mixing, the extra space is reduced, the reagents are saved, the flow rate is stabilized, and air bubbles are avoided.

[0051] In this embodiment, the four liquid inlet channels 122 and the four liquid outlet channels 123 are symmetrically distributed on both sides of the sample area 121. Each liquid inlet channel 122 and each liquid outlet channel 123 are substantially equal-width strip-shaped channels. The four liquid inlet channels 122 are divided into two groups, and two liquid inlet channels 122 in each group are arranged adjacent to each other. The two adjacent liquid inlet channels 122 in each group are connected at one end away from the liquid inlet port 113, the two groups of liquid inlet channels 122 are connected respectively and then connected at one end away from the liquid inlet port 113, and finally connected with the sample area 121. The structure of the four liquid outlet channels 123 is basically the same as that of the four liquid inlet channels 122, which will not be described again.

[0052] In this embodiment, the specific width of the four liquid inlet channels 122 and the four liquid outlet channels 123 can be determined according to the amount of reagents required for a single biochemical reaction, so as to avoid excessive waste of reagents. In this embodiment, the projection shape of the sample area 121 on the slide 11 is substantially circular, so as to adapt to the contour shape of the sample and the field of view range of the objective lens. In this embodiment, the area of the sample area 121 is slightly larger than the coverage range of the sample on the slide 11, so that the area of the sample area 121 is close to the coverage area of the sample on the slide 11. In this way, it is beneficial to fully utilize the reagents flowing into the sample area 121 and reduce the waste of reagents.

[0053] In other embodiments of the present application, the number of liquid inlet channels 122 and the number of liquid inlet ports 113 can not be in one-to-one correspondence, the number of liquid inlet channels 122 can be less than the number of liquid inlet ports 113, and then one liquid inlet channel 122 can be connected to more than one liquid inlet port 113, so that one liquid inlet channel 122 can be used for the flow of reagents from different liquid inlet ports 113 at different times.

[0054] In other embodiments of the present application, the number of liquid inlet 113 and liquid outlet 114 can not be one-to-one correspondence, the number of liquid inlet 113 can be more than the number of liquid outlet 114, and the number of liquid inlet 113 can also be less than the number of liquid outlet 114. In these embodiments, one liquid inlet 113 can correspond to multiple liquid outlets 114. Or in these embodiments, multiple liquid inlets 113 can correspond to the same liquid outlet 114. In other embodiments of the present application, multiple liquid inlets 113 and one liquid outlet 114 can be formed on the slide 11; in this way, multiple different reagents flow into the sample area 121 from different liquid inlets 113, and different reagent waste from the sample area 121 flows out through the same liquid outlet 114. This embodiment is advantageous to simplify the hollow pattern on the channel layer 12.

[0055] In other embodiments of the present application, the channel layer 12 can be other materials that do not react with reagents.

[0056] Please refer to Figure 2 and Figure 5 , the slide device 10 also includes a positioning clamp 14. The positioning clamp 14 is provided with a mounting opening 141 and multiple positioning holes 142. The mounting opening 141 is adapted to the outline of the slide 11, the channel layer 12 and the cover glass 13, so the mounting opening 141 is rectangular in this embodiment. The slide 11, the channel layer 12 and the cover glass 13 are clamped in the mounting opening 141.

[0057] In this example, the positioning clamp 14 is provided with three positioning holes 142, which are located at the edges of the mounting opening 141 and communicate with the mounting opening 141. In this embodiment, the positioning clamp 14 is used to assist in positioning the placement position of the slide device 10 as a whole through each positioning hole 142, to position the lap and fit position of the slide device 10 and the flow channel device 20, so that the slide device 10 and the flow channel device 20 are precisely lapped and fitted. In the fluorescence detection system 1, the placement angle and position accuracy of the slide device 10 is required to be high, so as to generate accurate fluorescence images subsequently. The positioning clamp 14 is advantageous for accurately positioning the angle and position of the slide device 10.

[0058] In other embodiments of the present application, when the fluorescence detection system has slightly lower requirements for the placement angle and position of the slide device 10, the slide device 10 can not include the positioning clamp 14.

[0059] Please refer to Figure 6The fluorescence detection system 1 of the embodiment of the present application comprises the slide device 10 described above, and further comprises a flow channel device 20 and a fluorescence imaging device 30. The flow channel device 20 is connected to the slide device 10, and is used to inject reagents into the slide device 10 during a biochemical process, and to recover reagent waste liquid from the slide device 10. The fluorescence imaging device 30 is used to emit laser light towards the sample in the slide device 10 after the biochemical process is completed, and to take a fluorescence image of the sample to obtain fluorescence distribution information of the sample 200.

[0060] Please refer to Figure 7 The flow channel device 20 comprises a reagent storage unit 21, a liquid inlet flow path unit 22, a liquid outlet flow path unit 23, and a waste liquid recovery unit 24. The liquid inlet flow path unit 22 is connected to the reagent storage unit 21 and the slide device 10 respectively, and the liquid outlet flow path unit 23 is connected to the waste liquid recovery unit 24 and the slide device 10 respectively. The reagent storage unit 21 is used to store reagents. The liquid inlet flow path unit 22 provides a flow channel for the reagents, and is used to guide the reagents stored in the reagent storage unit 21 to the slide device 10. After the reagents react with the sample in the slide device 10, the reagent waste liquid generated is flowed into the waste liquid recovery unit 24 through the liquid outlet flow path unit 23 for temporary storage and treatment.

[0061] In the embodiment, the flow channel device 20 further comprises a negative pressure driving unit 25 connected to the liquid outlet flow path unit 23, which is used to provide negative pressure so that the reagents can be forced to flow into the slide device 10, and so that the reagent waste liquid can be forced to flow into the waste liquid recovery unit 24.

[0062] In other embodiments of the present application, the flow channel device 20 can comprise a positive pressure driving unit connected to the liquid inlet flow path unit 22 instead of the negative pressure driving unit 25 described above, which is used to provide positive pressure so that the reagents can be forced to flow into the slide device 10, and so that the reagent waste liquid can be forced to flow into the waste liquid recovery unit 24.

[0063] In the embodiment, the reagents described above comprise fluorescent dyes, which are used to stain proteins and cell nuclei in the sample. In the embodiment, different reagents are required to stain different proteins and cell nuclei in the sample in sequence.

[0064] In the following, a slide (with a reflective layer 111 of metal chromium) of the embodiment of the present application and a common glass slide (without a reflective layer) are used to perform mouse brain paraffin section staining and photographing, which specifically comprises the following steps.

[0065] (1) A mouse brain paraffin block is taken, and a section with a thickness of 5 µm is cut, and the section is transferred to a water bath at 45°C for section development.

[0066] (2) After the section is developed and flattened, the slide of the embodiment of the present application and a common pathological adhesive glass slide are used to take the section respectively.

[0067] (3) Drying at 65°C for 1 hour.

[0068] (4) Put the slide with tissue into xylene and soak for 1 hour at room temperature for deparaffinization.

[0069] (5) Put the deparaffinized slide into 100%, 95%, 70%, 50% alcohol and pure water respectively for 20 minutes for gradient hydration to enhance the hydrophilicity of the tissue, which is beneficial for subsequent staining.

[0070] (6) Add 1:50000 diluted nuclear dye to the tissue on the slide. Incubate for 10 minutes at room temperature for staining.

[0071] (7) Put the slide into phosphate buffer solution (PBS) for 5 minutes at room temperature. The PBS buffer solution contains sodium chloride, potassium chloride, potassium dihydrogen phosphate and disodium hydrogen phosphate, and the pH value is between 7-7.4.

[0072] (8) Add imaging buffer to the tissue. Cover the slide with a cover glass for imaging.

[0073] (9) Use software to statistically analyze the photographed pictures.

[0074] Figure 8 The enlarged photo of the local nucleus staining of the mouse brain section on the slide (the reflective layer 111 with metal chromium) of the embodiment of the present application. After the sample on the slide (the reflective layer 111 with metal chromium) of the embodiment of the present application and the sample on the common glass slide (without the reflective layer 111) are photographed, 5 tissue fields of view (FOV) are selected respectively, and the average fluorescence intensity is counted. The results show that under the same conditions, the fluorescence signal intensity of the sample on the slide of the embodiment of the present application (about 24000) is significantly higher than that of the sample on the common glass slide (about 14000).

[0075] The slide 11 of the present application reflects the excitation light by setting the reflective layer 111, and the reflected excitation light will excite the sample twice, thereby increasing the excitation effect and improving the emission light signal intensity without increasing the power of the excitation light source. In addition, the reflective layer 111 is not transparent to light, so the light will not pass through the slide 11 to reflect on the bottom stage, which is better adapted to specific types of microscopic imaging platforms.

[0076] Those skilled in the art should understand that the above embodiments are only used to illustrate the present application, but not as a limitation to the present application, and any appropriate changes and variations to the above embodiments within the spirit and scope of the present application fall within the scope of the present application.

Claims

1. A carrier, which is in the form of a sheet as a whole, characterized in that, The slide comprises: a body layer; and a reflective layer laminated on a surface of the body layer, the reflective layer configured to reflect light incident on the slide and to reflect fluorescence emitted by a cell or tissue sample.

2. The slide of claim 1, wherein, The reflective layer has a thickness of 150-200 nm.

3. The slide of claim 1, wherein, The slide further comprises a functional modification layer laminated on a side of the reflective layer facing away from the body layer, the functional modification layer configured to increase adhesion of the cell or tissue sample to the slide.

4. The slide of claim 3, wherein, The functional modification layer has a thickness of less than 100 µm.

5. The slide of claim 1, wherein, The slide has opposite first and second surfaces, wherein the second surface is a surface of the body layer facing away from the reflective layer, the slide further has at least one inlet port and at least one outlet port, the at least one inlet port and the at least one outlet port respectively penetrating the first and second surfaces.

6. The slide of claim 5, wherein, The slide is a rectangular sheet, the at least one inlet port and the at least one outlet port are respectively located at opposite sides of the slide.

7. A slide device comprising a slide, characterized in that The slide is any one of claims 1-6.

8. The slide device of claim 7, wherein, The slide device further comprises a cover glass and a channel layer laminated on the slide, the channel layer is located between the cover glass and the slide.

9. The slide device of claim 8, wherein, The channel layer is connected to a first surface of the slide, the first surface is a surface of the slide opposite to the surface of the body layer facing away from the reflective layer, the channel layer has a sample area, at least one inlet channel and at least one outlet channel, the at least one inlet channel is in communication with the at least one inlet port and the sample area, the at least one outlet channel is in communication with the at least one outlet port and the sample area, the first surface is exposed to the channel layer through the sample area, the at least one inlet channel and the at least one outlet channel.

10. A fluorescence detection system characterized by, The slide device comprises: the slide as claimed in any one of claims 7-9; a flow channel device in communication with the at least one inlet port of the slide and the at least one outlet port of the slide respectively, configured to deliver reagents to the at least one outlet port and to recover reagent waste from the at least one outlet port; and a fluorescence imaging device configured to excite reagents in the sample area of the slide device and to receive fluorescence generated by the reagents to generate a fluorescence image of the sample. The slide device comprises: the slide as claimed in any one of claims 7-9; a flow channel device in communication with the at least one inlet port of the slide and the at least one outlet port of the slide respectively, configured to deliver reagents to the at least one outlet port and to recover reagent waste from the at least one outlet port; and a fluorescence imaging device configured to excite reagents in the sample area of the slide device and to receive fluorescence generated by the reagents to generate a fluorescence image of the sample.

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