A high-efficiency separation device for marine fish collagen oligopeptides

Abstract

The utility model discloses a kind of high-efficiency separation devices of marine fish collagen oligopeptide, comprising: processing kettle, it is reaction kettle structure, the processing kettle upper end opening is equipped with pretreatment box, and pretreatment box receives the mixture from marine fish collagen oligopeptide raw material enzymolysis reaction, is the early treatment for the separation of marine fish collagen oligopeptide.This high-efficiency separation device of marine fish collagen oligopeptide, double-layer separation structure is constituted with gel filtration column and ion exchange resin in device, realize the high-efficiency, high-precision separation of marine fish collagen oligopeptide, the number of gel filtration column set during separation is associated according to the total amount of solution handled, by increasing the number of gel filtration column, it is convenient for a large amount of solution to carry out production separation, water bath temperature environment is constructed simultaneously in the periphery of gel filtration column, so that marine fish collagen oligopeptide and ion activity on ion exchange resin are enhanced, so that ion diffusion speed in ion exchange process is accelerated.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological separation technical field, concretely is a kind of high-efficiency separation device of marine fish collagen oligopeptide. BACKGROUND

[0002] Marine fish collagen oligopeptide is a kind of small molecule peptide with high biological activity. It contains rich glycine, proline, hydroxyproline and other amino acids, and these amino acids have important role for the repair and maintenance of human skin, bone, joint and other tissues, and it can promote the synthesis of skin collagen, increase the elasticity and moisture retention capacity of skin, reduce the generation of wrinkle, is the key component of many high-end skin care products and beauty health care products, and marine fish collagen oligopeptide is helpful for wound healing, and has certain efficacy for the auxiliary treatment of some joint diseases, with the continuous increase of people's demand for health and beauty, the market demand for high-quality marine fish collagen oligopeptide continues to rise.

[0003] In the production of marine fish collagen oligopeptide, it is necessary to carry out enzymatic treatment to promote the hydrolysis of collagen protein and further improve the yield of small molecule fish collagen oligopeptide. The mixture obtained after enzymolysis needs to be separated to further purify fish collagen oligopeptide. The traditional separation method separates the peptide by salting out or organic solvent precipitation, but the selectivity is poor, and other impurities are often precipitated at the same time, resulting in low product purity. The subsequent impurity removal step is complex, which can cause loss of peptide and reduce the yield of product. However, the cost of chromatographic separation method for large amount of mixture is increased, which is difficult to meet the production needs. UTILITY MODEL CONTENT

[0004] The utility model aims at providing a kind of high-efficiency separation device of marine fish collagen oligopeptide to solve the problem that traditional separation method separates the peptide by salting out or organic solvent precipitation, but the selectivity is poor, and other impurities are often precipitated at the same time, resulting in low product purity.

[0005] To achieve the above purpose, the utility model provides the following technical scheme: a kind of high-efficiency separation device of marine fish collagen oligopeptide, including processing kettle, it is reaction kettle structure, the processing kettle upper end opening place is equipped with pretreatment box, and pretreatment box receives the mixture from marine fish collagen oligopeptide raw material enzymolysis reaction, is the front processing of marine fish collagen oligopeptide separation, and processing kettle is the main shell structure of device;

[0006] The processing kettle is internally provided with a concentration hopper, and the upper end of the concentration hopper is hingedly connected with a pretreatment box; one side of the processing kettle is provided with a central processing unit; the side wall of the pipeline at the bottom end of the concentration hopper is provided with a flow detector, a pH sensor and a temperature sensor, and the flow detector, the pH sensor and the temperature sensor are connected; the bottom end of the concentration hopper is connected with a filtering and shunting hopper through a pipeline, and the bottom end of the filtering and shunting hopper is connected with a separation assembly; and the enzymolysis mixture after preliminary filtration is further separated to obtain marine fish collagen oligopeptide.

[0007] The pretreatment box is provided with a U-shaped box body in cross section, and a plurality of fan-shaped openings are symmetrically arranged at the bottom end of the pretreatment box; a lifting column is installed in the pretreatment box, and a pressurizing plate is connected to the output end of the lifting column, and the lifting column penetrates through a filter membrane group; the filter membrane group is installed inside the pretreatment box, and the filter membrane group is arranged in parallel with the pressurizing plate.

[0008] The above technical scheme can keep the quality and performance of the marine fish collagen oligopeptide obtained by separation highly consistent.

[0009] Preferably, the pretreatment box and the concentration hopper are arranged in a nested manner, and the top end of the pretreatment box is arranged at the top end of the processing kettle.

[0010] The above technical scheme can prevent the installation positions of the pretreatment box and the concentration hopper from being in conflict.

[0011] Preferably, the concentration hopper and the filtering and shunting hopper are arranged in a funnel shape, and the opening at the bottom end of the filtering and shunting hopper abuts against the inner wall of the processing kettle.

[0012] The above technical scheme can facilitate the diversion of liquid to the periphery through the combined installation of the concentration hopper and the filtering and shunting hopper.

[0013] Preferably, the separation assembly comprises:

[0014] A first limiting sealing plate is installed on the inner wall of the processing kettle, and the first limiting sealing plate is arranged at the lower end of the filtering and shunting hopper;

[0015] A gel filtration column is arranged in a matrix shape inside the processing kettle, and the top end of the gel filtration column penetrates through the first limiting sealing plate;

[0016] A second limiting sealing plate is installed on the inner wall of the processing kettle, and the first limiting sealing plate is penetrated by the bottom end of the gel filtration column;

[0017] An ion exchange resin is placed inside the gel filtration column, and the ion exchange resin is placed close to the lower part of the gel filtration column.

[0018] The above technical scheme can be used for molecular separation of marine fish collagen oligopeptide.

[0019] Preferably, the limiting sealing plate one top end surface is flush with the top end of the gel filtration column, and the gel filtration column is filled with cross-linked polysaccharide gel particles.

[0020] With the above technical scheme, the flush of the limiting sealing plate one with the gel filtration column facilitates the mixed liquid to enter the gel filtration column.

[0021] Preferably, the limiting sealing plate one and the limiting sealing plate two divide the internal chamber of the treatment kettle into three layers, and the chamber of the middle layer of the treatment kettle is connected with the water inlet pipe and the water outlet pipe.

[0022] With the above technical scheme, the installation of the limiting sealing plate one and the limiting sealing plate two separates the internal space of the treatment kettle, facilitating accurate processing of the internal structure.

[0023] Preferably, the water inlet pipe and the water outlet pipe both penetrate the treatment kettle, and the installation height of the water inlet pipe is higher than that of the water outlet pipe.

[0024] With the above technical scheme, the water inlet pipe and the water outlet pipe are arranged to build a water bath auxiliary environment with uniform heating and constant temperature in the treatment kettle, facilitating the separation of marine fish collagen oligopeptide.

[0025] Compared with the prior art, the marine fish collagen oligopeptide high-efficiency separation device has the advantages that:

[0026] 1. The device adopts a double-layer separation structure composed of a gel filtration column and an ion exchange resin to realize high-efficiency and high-precision separation of marine fish collagen oligopeptide. The number of gel filtration columns is associated with the total amount of solution to be processed. By increasing the number of gel filtration columns, a large amount of solution can be separated, and a water bath temperature environment is constructed around the gel filtration columns to enhance the ion activity of marine fish collagen oligopeptide and ion exchange resin, accelerate the ion diffusion speed in the ion exchange process, and enhance the selectivity of ion exchange resin to different oligopeptides, thereby making the product separation and screening process more accurate.

[0027] 2. The use of a pretreatment box can preliminarily filter macromolecules in the solution after enzymatic hydrolysis, reduce solid impurities and macromolecular impurities, and reduce the burden on the gel filtration column. The detachable pretreatment box facilitates quick replacement of the filter membrane group during production, avoids macromolecular interception filtration process, and enables the separation process to be carried out quickly. Compared with traditional separation devices, the device can shorten the separation time by several times. BRIEF DESCRIPTION OF DRAWINGS

[0028] Figure 1 It is a schematic diagram of the overall external three-dimensional structure of the utility model;

[0029] Figure 2 It is the whole internal structure structure schematic diagram of the utility model;

[0030] Figure 3 It is the processing kettle and gel filtration column installation three-dimensional structure schematic diagram of the utility model;

[0031] Figure 4 It is the internal right section three-dimensional structure schematic diagram of the utility model pretreatment box;

[0032] Figure 5 It is the centralized bucket, filter shunt bucket and temperature sensor installation three-dimensional structure schematic diagram of the utility model;

[0033] Figure 6 It is the limiting sealing plate two and ion exchange resin installation three-dimensional structure schematic diagram of the utility model.

[0034] In the drawing: 1, processing kettle;2, pretreatment box;3, lifting column;4, pressurizing plate;5, filter membrane group;6, centralized bucket;7, filter shunt bucket;8, flow detector;9, pH sensor;10, temperature sensor;11, central processing unit;12, limiting sealing plate one;13, gel filtration column;14, limiting sealing plate two;15, ion exchange resin;16, water inlet pipe;17, water outlet pipe. DETAILED DESCRIPTION

[0035] The technical scheme in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the scope of protection of the utility model.

[0036] Please refer to Figures 1-6 The utility model provides a kind of technical scheme: a kind of high-efficiency separation device of marine fish collagen oligopeptide, including processing kettle 1, pretreatment box 2, lifting column 3, pressurizing plate 4, filter membrane group 5, centralized bucket 6, filter shunt bucket 7, flow detector 8, pH sensor 9, temperature sensor 10, central processing unit 11, limiting sealing plate one 12, gel filtration column 13, limiting sealing plate two 14, ion exchange resin 15, water inlet pipe 16 and water outlet pipe 17;

[0037] Wherein, processing kettle 1, it is reaction kettle structure, processing kettle 1 upper end opening is equipped with pretreatment box 2, and pretreatment box 2 receives the mixture from marine fish collagen oligopeptide raw material enzymolysis reaction, is the early treatment for the separation of marine fish collagen oligopeptide, and processing kettle 1 is the main shell structure of device;

[0038] The inside of the treatment kettle 1 is provided with a collecting hopper 6, and the upper end of the collecting hopper 6 is hingedly connected with the pretreatment box 2. The treatment kettle 1 is provided with a central processing unit 11 on one side. The sidewall of the pipeline at the bottom end of the collecting hopper 6 is provided with a flow detector 8, a pH sensor 9 and a temperature sensor 10, and the flow detector 8, the pH sensor 9 and the temperature sensor 10 are connected with the central processing unit 11 through cables. The bottom end of the collecting hopper 6 is connected with the filtering and shunting hopper 7 through a pipeline, and the bottom end of the filtering and shunting hopper 7 is connected with the separation assembly. The enzyme hydrolysis mixture subjected to preliminary filtration is further separated to obtain marine fish collagen oligopeptide. The collecting hopper 6 and the filtering and shunting hopper 7 are provided in a funnel shape, and the opening at the bottom end of the filtering and shunting hopper 7 abuts against the inner wall of the treatment kettle 1.

[0039] The pretreatment box 2 is provided in a U-shaped cross section. The bottom end of the pretreatment box 2 is symmetrically provided with a fan-shaped opening. The pretreatment box 2 is provided with a lifting column 3. The output end of the lifting column 3 is connected with a pressurizing plate 4. The lifting column 3 penetrates through a filter membrane group 5. The filter membrane group 5 is installed inside the pretreatment box 2. The filter membrane group 5 is provided in parallel with the pressurizing plate 4. The pretreatment box 2 is provided in a nested manner with the collecting hopper 6. The top end of the pretreatment box 2 is installed with the top end of the treatment kettle 1.

[0040] The specific embodiments of the application are described in detail in the following Figures 1-6 The pretreatment box 2 is placed in the opening at the top of the treatment kettle 1 and is nested with the collecting hopper 6. As shown in Figure 2 When used, the solid-liquid mixture after enzyme hydrolysis is sent into the pretreatment box 2. The solid is intercepted and filtered by the filter membrane group 5. The filter membrane group 5 is composed of a plurality of ultrafine filter membranes stacked together. The filter membrane group 5 in the pretreatment box 2 is replaced at intervals to maintain the production quality of marine fish collagen oligopeptide. The pressurizing plate 4 driven by the lifting column 3 is lowered to press the solid residues on the surface of the filter membrane group 5 to further compress and squeeze the liquid, thereby reducing material waste. The funnel-shaped arrangement of the collecting hopper 6 and the filtering and shunting hopper 7 is used to limit the flow direction of the liquid. The filtering and shunting hopper 7 is internally provided with a grid plate stacked and installed, which is used to shunt the concentrated liquid downward to the gel filtration column 13. The solution passes through the pipeline connected between the collecting hopper 6 and the filtering and shunting hopper 7, so that the solution is synchronously detected by the flow detector 8, the pH sensor 9 and the temperature sensor 10. The state of the solution is recorded. The data state during product production is detected. The central processing unit 11 is used to normalize the data sources and send data to the detection workshop. With the funnel-shaped structure of the filtering and shunting hopper 7, the liquid is conveniently sent to the surrounding gel filtration column 13.

[0041] The separation assembly comprises:

[0042] A limiting sealing plate one 12 is installed on the inner wall of the treatment kettle 1, and the limiting sealing plate one 12 is arranged at the lower end of the filtering and shunting hopper 7, the top surface of the limiting sealing plate one 12 is flush with the top end of the gel filtration column 13, the inside of the gel filtration column 13 is filled with cross-linked polysaccharide gel particles, the limiting sealing plate one 12 and the limiting sealing plate two 14 divide the internal cavity of the treatment kettle 1 into three layers, and the cavity in the middle layer of the treatment kettle 1 is connected with the water inlet pipe 16 and the water outlet pipe 17, the water inlet pipe 16 and the water outlet pipe 17 both penetrate the treatment kettle 1, and the installation height of the water inlet pipe 16 is higher than that of the water outlet pipe 17;

[0043] The gel filtration column 13 is arranged in a matrix in the inside of the treatment kettle 1, and the top end of the gel filtration column 13 penetrates the limiting sealing plate one 12;

[0044] The limiting sealing plate two 14 is installed on the inner wall of the treatment kettle 1, and the limiting sealing plate one 12 is penetrated by the bottom end of the gel filtration column 13;

[0045] The ion exchange resin 15 is placed in the gel filtration column 13, and the ion exchange resin 15 is placed close to the lower part of the gel filtration column 13;

[0046] The combination of the description and the drawings Figures 1-6 As shown in the drawings, the parallel installation of the limiting sealing plate one 12 and the limiting sealing plate two 14 further separates the internal space of the treatment kettle 1, and the limiting sealing plate one 12 and the limiting sealing plate two 14 provide limiting for the installation of the gel filtration column 13, wherein the gel filtration column 13 is located in the cavity in the middle layer of the treatment kettle 1, as shown in the drawings, Figures 2-3 As shown in the drawings, when in use, the water inlet pipe 16 is connected through the external pipeline to send hot water with temperature into the middle layer space of the treatment kettle 1, and the gate is connected and installed with the water outlet pipe 17 for exchanging the hot water to maintain the temperature inside the treatment kettle 1, the temperature of the hot water enhances the ion activity of the marine fish collagen oligopeptide mixed solution flowing in the gel filtration column 13 and the ion exchange resin, accelerates the ion diffusion speed in the ion exchange process, and influences the charge distribution and conformation of the marine fish collagen oligopeptide to enhance the selectivity of the ion exchange resin to different oligopeptides, and the corresponding ion exchange resin 15 is replaced according to the production needs of the marine fish collagen oligopeptide when in use, the ion exchange resin 15 adopts cation exchange resin, anion exchange data, affinity ion exchange resin or chelating ion exchange resin, which can be further separated and purified according to the charge characteristics of the marine fish collagen oligopeptide to obtain marine fish collagen oligopeptide with the required molecular weight, and the arrangement of multiple gel filtration columns 13 facilitates the batch processing of a large amount of mixture.

[0047] Working principle: when using the high-efficiency separation device of the marine fish collagen oligopeptide, the enzymatic mixture is poured from the opening between the pretreatment box 2 and the pressurizing plate 4, and is preliminarily filtered through the filter membrane group 5, then the lifting column 3 is started to lower the pressurizing plate 4 into the pretreatment box 2 and cooperate with the filter membrane group 5 to further extrude and filter the solids in the enzymatic mixture, and the liquid is concentrated into the concentration bucket 6 and passes through the detection pipeline into the filter shunt bucket 7, and the liquid is synchronously detected by the flow detector 8, the pH sensor 9 and the temperature sensor 10 during the pipeline flow between the concentration bucket 6 and the filter shunt bucket 7, and the detection data is uploaded to the central processing unit 11 for calculation, and the filtered liquid is sent into the gel filtration column 13 by the filter shunt bucket 7, and is treated by the crosslinked polysaccharide gel particles and the ion exchange resin 15, so that different molecules are separated, and the molecules in the solution are further separated by cooperating with the ion exchange resin 15, so that the product with a specific molecular weight is obtained, and the gel filtration column 13 is used for exchange filtration, the cavity around the gel filtration column 13 is filled with liquid at a suitable temperature, and the reaction substance in the gel filtration column 13 is provided with a suitable temperature separation environment, the exchange rate is improved, the charge distribution and conformation of the marine fish collagen oligopeptide are further affected, the sorting of the marine fish collagen oligopeptide is facilitated, and the overall practicability is improved.

[0048] Although the embodiments of the present application have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to the embodiments without departing from the principles and spirits of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A high-efficiency separation device for marine fish collagen oligopeptide, comprising: a treatment kettle (1) which is a reaction kettle structure, a pretreatment box (2) is installed at the open upper end of the treatment kettle (1), and the pretreatment box (2) receives the mixture from the enzymatic reaction of the marine fish collagen oligopeptide raw material, which is the preliminary treatment for the separation of the marine fish collagen oligopeptide, and the treatment kettle (1) is the main shell structure of the device; characterized in that: a concentration bucket (6) is arranged inside the treatment kettle (1), and the upper end of the concentration bucket (6) is connected with the pretreatment box (2) in a clamping manner, a central processing unit (11) is arranged on one side of the treatment kettle (1), a flow detector (8), a pH sensor (9) and a temperature sensor (10) are arranged on the side wall of the pipeline at the bottom end of the concentration bucket (6), and the flow detector (8), the pH sensor (9) and the temperature sensor (10) are connected with the central processing unit (11) through cables, the bottom end of the concentration bucket (6) is connected with a filtering and shunting bucket (7) through a pipeline, and the bottom end of the filtering and shunting bucket (7) is connected with a separation assembly, so as to further separate the enzymatic mixture after preliminary filtration to obtain marine fish collagen oligopeptide; the pretreatment box (2) is arranged in the shape of a U-shaped box in cross section, and a fan-shaped opening is symmetrically arranged at the bottom end of the pretreatment box (2), a lifting column (3) is installed in the pretreatment box (2), a pressurizing plate (4) is connected to the output end of the lifting column (3), and the lifting column (3) penetrates through a filter membrane group (5), and the filter membrane group (5) is installed inside the pretreatment box (2) and arranged in parallel with the pressurizing plate (4).

2. The device for efficient separation of marine fish collagen oligopeptides according to claim 1, characterized in that: the pretreatment box (2) and the concentration bucket (6) are arranged in a nested manner, and the top end of the pretreatment box (2) is arranged in the top end of the treatment kettle (1).

3. The device for efficient separation of marine fish collagen oligopeptides according to claim 1, characterized in that: the concentration bucket (6) and the filtering and shunting bucket (7) are arranged in the shape of a funnel, and the bottom opening of the filtering and shunting bucket (7) abuts against the inner wall of the treatment kettle (1).

4. The device for efficient separation of marine fish collagen oligopeptides according to claim 1, characterized in that: the separation assembly comprises: a limiting sealing plate one (12) which is installed on the inner wall of the treatment kettle (1) and arranged at the lower end of the filtering and shunting bucket (7); a gel filtration column (13) which is arranged in a matrix shape inside the treatment kettle (1), and the top end of the gel filtration column (13) penetrates through the limiting sealing plate one (12); a limiting sealing plate two (14) which is installed on the inner wall of the treatment kettle (1) and penetrated by the bottom end of the gel filtration column (13); an ion exchange resin (15) which is placed inside the gel filtration column (13) and placed close to the lower part of the gel filtration column (13).

5. The device for efficient separation of marine fish collagen oligopeptides according to claim 4, characterized in that: the top end surface of the limiting sealing plate one (12) is flush with the top end of the gel filtration column (13), and the gel filtration column (13) is filled with cross-linked polysaccharide gel particles.

6. The device for efficient separation of marine fish collagen oligopeptides according to claim 4, characterized in that: the limiting sealing plate one (12) and the limiting sealing plate two (14) divide the internal chamber of the treatment kettle (1) into three layers, and the chamber in the middle layer of the treatment kettle (1) is connected with a water inlet pipe (16) and a water outlet pipe (17).

7. The device for efficient separation of marine fish collagen oligopeptides according to claim 6, characterized in that: The water inlet pipe (16) and the water outlet pipe (17) both penetrate the treatment kettle (1), and the installation height of the water inlet pipe (16) is higher than that of the water outlet pipe (17).

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