Gradient PCR instrument convenient to maintain and overhaul

Abstract

The utility model relates to the technical field of gradient PCR (Polymerase Chain Reaction) instruments, in particular to a gradient PCR instrument convenient to maintain and overhaul, and solves the problems that a gradient PCR instrument sample pool in the prior art is integrated with an instrument body and lacks a detachable function, so that the maintenance efficiency and the maintenance effect of the gradient PCR instrument sample pool are greatly reduced. A gradient PCR instrument convenient to maintain and overhaul comprises a gradient PCR instrument body, a sample pool is formed in one side of the top of the gradient PCR instrument body, a sample frame is arranged on the inner side of the sample pool, the bottom of the sample frame is elastically connected with the bottom of the inner side of the sample pool, an anti-skid frame is fixedly connected to the inner side of the sample frame through bolts, and the anti-skid frame is fixedly connected with the outer side of the sample pool through bolts. And connecting rods are fixedly connected to four corners of the inner side of the sample frame. According to the gradient PCR instrument sample pool, the problem that a traditional gradient PCR instrument sample pool is inconvenient to maintain and overhaul is solved, the maintenance efficiency is improved, the maintenance effect is enhanced, the operation convenience is improved, and the service life of the instrument is prolonged.

Description

Technical Field

[0001] This utility model relates to the field of gradient PCR instrument technology, and in particular to a gradient PCR instrument that is easy to maintain and repair. Background Technology

[0002] Gradient PCR instruments are advanced molecular biology experimental instruments that optimize PCR reaction conditions by setting multiple temperature gradients within the same reaction, thereby improving amplification efficiency and specificity. They can rapidly screen for the optimal annealing temperature and are suitable for various fields such as gene expression analysis, disease diagnosis, gene cloning and identification, and agricultural applications. Gradient PCR instruments employ a high-precision temperature control system and rapid heating and cooling technology to ensure the stability and accuracy of PCR reactions. They also support various experimental templates and custom programs, allowing users to quickly set up different experimental designs. Their wide range of applications provides crucial technical support for research and development in life sciences, medicine, agriculture, and other fields.

[0003] For maintenance and repair of the sample cells in a gradient PCR instrument, first turn off the PCR instrument and allow it to cool for a period of time. Then, open the lid and soak the sample cells in 95% ethanol or 10% cleaning solution for 5 minutes. Next, clean the contaminated wells, aspirate liquid with a micropipette, and blot away any remaining liquid with a cotton swab. Then, turn on the PCR instrument, set the PCR program to a constant temperature of 50°C, and run it to allow residual liquid to evaporate and be removed, which usually takes 5-10 minutes. If the contaminants are not effectively removed, repeat the above steps.

[0004] When performing maintenance on the sample pool of a gradient PCR instrument, disassembly of the sample pool is crucial to ensure effective maintenance. However, traditional gradient PCR instrument sample pools are integrated with the instrument body and lack detachability, which significantly reduces the efficiency and effectiveness of maintenance and is extremely inconvenient. Therefore, there is an urgent need for a gradient PCR instrument that is easy to maintain and repair to solve the above problems. Utility Model Content

[0005] The purpose of this invention is to provide a gradient PCR instrument that is easy to maintain and repair. This solves the problem that in the prior art, the sample pool of the gradient PCR instrument is integrated with the instrument body and lacks a detachable function, which greatly reduces the maintenance efficiency and effectiveness of the sample pool.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A gradient PCR instrument that is easy to maintain and repair includes a gradient PCR instrument body. A sample pool is provided on one side of the top of the gradient PCR instrument body, and a sample frame is provided inside the sample pool. The bottom of the sample frame is elastically connected to the bottom of the inner side of the sample pool. An anti-slip frame is fixedly connected to the inner side of the sample frame by bolts. Connecting rods are fixedly connected to the four corners of the inner side of the sample frame. Two of the four connecting rods on the same side are fixedly connected to each other by a handle rod. Insert rods are provided on both sides of the gradient PCR instrument body, and one end of each insert rod penetrates the side wall of the gradient PCR instrument body. Docking blocks are fixedly connected to both sides of the bottom of the sample frame, and slots for use with the insert rods are provided on one side of each docking block.

[0008] Preferably, several compression springs are fixedly connected to the bottom of both sides of the sample frame, and all compression springs are in contact with the bottom of the inner side of the sample cell.

[0009] Preferably, the bottom of the sample cell has docking grooves on both sides for use with two docking blocks.

[0010] Preferably, the top of the gradient PCR instrument body is hinged to a cover plate, and the cover plate is located on top of the sample cell.

[0011] Preferably, a tension spring is fitted on one end of each of the two insertion rods, and one end of the insertion rod is elastically connected to the outer wall of the gradient PCR instrument body through the tension spring.

[0012] Preferably, handle grooves are provided on both sides of the sample frame.

[0013] This utility model has the following beneficial effects:

[0014] By designing a detachable sample frame and insert rod limiting structure, the cleaning and maintenance of the sample cell becomes simpler and faster. Operators can easily remove the sample frame for cleaning without complicated tools or procedures, greatly improving maintenance efficiency. The detachable design of the sample frame and anti-slip frame allows for more thorough cleaning. Operators can perform detailed cleaning and disinfection of the sample frame and anti-slip frame separately, ensuring the cleanliness and hygiene of the sample cell, thereby improving the accuracy and reliability of PCR experiments. The insertion design of the insert rod and docking block is simple and practical. Operators only need to pull the insert rod to fix and release the sample frame, without additional tools or complicated operating procedures, reducing the difficulty of operation and time costs. The compression spring design between the bottom of the sample frame and the inner bottom of the sample cell not only facilitates the extraction of the sample frame, but also provides stable support when reassembling, ensuring the stability of the sample frame during PCR experiments and preventing sample displacement or damage. This utility model solves the problem of inconvenient maintenance and repair of the sample cell in traditional gradient PCR instruments, improves maintenance efficiency, enhances maintenance effect, improves operational convenience, maintains instrument stability, and extends the service life of the instrument. Attached Figure Description

[0015] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of the overall appearance and structure of the present utility model;

[0017] Figure 2 This is a schematic diagram of the cover plate opening structure of this utility model;

[0018] Figure 3 This is a top view of the sample cell structure of this utility model;

[0019] Figure 4 This is a side view of the sample cell structure of this utility model;

[0020] Figure 5 This is a schematic diagram of the sample frame and its structure of the present invention.

[0021] In the diagram: 1. Gradient PCR instrument body; 2. Cover plate; 3. Insert rod; 4. Tension spring; 5. Sample frame; 6. Handle groove; 7. Anti-slip frame; 8. Connecting rod; 9. Grip rod; 10. Sample cell; 11. Docking block; 12. Docking groove; 13. Compression spring; 14. Slot. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model.

[0023] Reference Figure 1-5. A gradient PCR instrument that is easy to maintain and repair includes a gradient PCR instrument body 1. A sample pool 10 is provided on one side of the top of the gradient PCR instrument body 1, and a sample frame 5 is provided on the inner side of the sample pool 10. The bottom of the sample frame 5 is elastically connected to the bottom of the inner side of the sample pool 10. This design allows the sample frame 5 to be easily popped out when needed, facilitating the operator's sample handling and cleaning, and improving maintenance convenience. An anti-slip frame 7 is fixedly connected to the inner side of the sample frame 5 by bolts, and connecting rods 8 are fixedly connected to the four corners of the inner side of the sample frame 5. Two of the four connecting rods 8 located on the same side are fixedly connected by a handle rod 9. Insert rods 3 are provided on both sides of the gradient PCR instrument body 1, and two... One end of each insertion rod 3 penetrates the side wall of the gradient PCR instrument body 1. Both sides of the bottom of the sample frame 5 are fixedly connected to docking blocks 11, and each docking block 11 has a slot 14 on one side for use with the insertion rod 3. Insertion rods 3 are located on both sides of the gradient PCR instrument body 1, with one end of each insertion rod 3 penetrating the side wall of the gradient PCR instrument body 1. These insertion rods engage with the slots 14 on the docking blocks 11 fixedly connected to the bottom of the sample frame 5, achieving stable fixation of the sample frame 5 in the sample pool 10. This prevents the sample frame 5 from shaking or shifting during the experiment, ensuring experimental accuracy. Furthermore, when the sample frame 5 needs to be removed for maintenance, simply pulling the insertion rod 3 releases the fixation of the sample frame 5, making the operation simple and quick.

[0024] Furthermore, several compression springs 13 are fixedly connected to the bottom of both sides of the sample frame 5, and all compression springs 13 are in contact with the bottom of the inner side of the sample pool 10. When the insertion rod 3 releases the limiting position of the docking block 11, these compression springs 13 can quickly lift the sample frame 5 to a height that is easy to operate. This makes the sample frame 5 easier for the operator to grasp and extract, thereby further improving the convenience of maintenance and repair.

[0025] Furthermore, the bottom sides of the sample pool 10 are respectively provided with docking grooves 12 for use with two docking blocks 11. When the sample frame 5 is put back into the sample pool 10, the docking blocks 11 can accurately fit into the docking grooves 12, providing stable support for the sample frame 5. This enhances the stability of the sample frame 5 in the sample pool 10 and avoids displacement or shaking during the PCR experiment.

[0026] Furthermore, the top of the gradient PCR instrument body 1 is connected to a cover plate 2 via a hinge, and the cover plate 2 is located on top of the sample pool 10.

[0027] Furthermore, a tension spring 4 is fitted on one end of each of the two insertion rods 3, and one end of the insertion rod 3 is elastically connected to the outer wall of the gradient PCR instrument body 1 through the tension spring 4. The tension spring 4 can automatically pull the insertion rod 3 back to its original position, so that its end can be reinserted into the slot 14 of the docking block 11. This ensures that the insertion rod 3 can automatically reset after the limit is released, thereby simplifying the operation process and improving the efficiency of maintenance and repair.

[0028] Furthermore, handle grooves 6 are provided on both sides of the sample frame 5, allowing operators to insert their fingers into the handle grooves 6 to lift or lower the sample frame 5 more easily.

[0029] In summary:

[0030] The gradient PCR instrument body 1 is model 9700. First, ensure the PCR instrument is turned off and has cooled sufficiently to a safe temperature for subsequent maintenance and repair. Next, the operator opens the cover 2 on the top of the gradient PCR instrument body 1, which is hinged to the top, exposing the sample chamber 10 and the sample frame 5 inside. At this time, the bottom of the sample frame 5 is elastically connected to the bottom of the sample chamber 10 by several compression springs 13. These compression springs 13 are all tightly fitted to the bottom of the sample chamber 10, providing power for the ejection of the sample frame 5. Subsequently, the operator manually pulls the insert rods 3 on both sides of the gradient PCR instrument body 1, causing the ends of the insert rods 3 to retract from the slots 14 of the docking blocks 11 fixedly connected to the bottom sides of the sample frame 5. This action releases the insertion limit of the insert rods 3 to the docking blocks 11, and simultaneously, due to the elastic force of the compression springs 13, the sample frame 5 will quickly spring up to a height suitable for operation. At this point, the operator can easily insert their fingers into the handle slots 6 on both sides of the sample frame 5 to lift the sample frame 5 more effortlessly for subsequent cleaning and maintenance. During cleaning, if a more thorough cleaning is required, the operator can also separately remove the anti-slip frame 7, which is bolted to the inside of the sample frame 5, for cleaning. The design of the anti-slip frame 7 not only enhances the stability of the sample frame 5 but also facilitates disassembly and cleaning, maintaining the cleanliness of the sample chamber 10. After cleaning, the operator reassembles the anti-slip frame 7 and the sample frame 5 into the sample chamber 10 in reverse order. During reassembly, the docking block 11 accurately engages with the docking slots 12 on both sides of the bottom of the sample chamber 10, providing stable support for the sample frame 5 and preventing displacement or shaking during PCR experiments. Simultaneously, tension springs 4 are fitted onto one end of each of the two insertion rods 3, maintaining an elastic connection between the insertion rods 3 and the outer wall of the gradient PCR instrument body 1 via the tension springs 4. When the operator releases the insertion rod 3, the tension spring 4 can automatically pull the insertion rod 3 back to its original position, so that its end can be reinserted into the slot 14 of the docking block 11. This ensures that the insertion rod 3 can automatically reset after the limit is released, thereby simplifying the operation process and improving the efficiency of maintenance and repair.

[0031] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A gradient PCR instrument that is easy to maintain and repair, comprising a gradient PCR instrument body (1), characterized in that, The gradient PCR instrument body (1) has a sample pool (10) on one side of its top, and a sample frame (5) is provided inside the sample pool (10). The bottom of the sample frame (5) is elastically connected to the bottom of the sample pool (10). An anti-slip frame (7) is fixedly connected to the inside of the sample frame (5) by bolts. Connecting rods (8) are fixedly connected to the four corners of the inside of the sample frame (5). Two of the four connecting rods (8) located on the same side are fixedly connected by a handle rod (9). Insert rods (3) are provided on both sides of the gradient PCR instrument body (1), and one end of each of the two insert rods (3) penetrates the side wall of the gradient PCR instrument body (1). A docking block (11) is fixedly connected to both sides of the bottom of the sample frame (5), and a slot (14) for use with the insert rod (3) is provided on one side of each of the two docking blocks (11).

2. The gradient PCR instrument for easy maintenance and repair according to claim 1, characterized in that, Several compression springs (13) are fixedly connected to the bottom of both sides of the sample frame (5), and all compression springs (13) are in contact with the bottom of the inner side of the sample pool (10).

3. The gradient PCR instrument for easy maintenance and repair according to claim 1, characterized in that, The bottom sides of the sample cell (10) are respectively provided with docking grooves (12) for use with two docking blocks (11).

4. A gradient PCR instrument for easy maintenance and repair according to claim 1, characterized in that, The top of the gradient PCR instrument body (1) is connected to a cover plate (2) by a hinge, and the cover plate (2) is located on top of the sample pool (10).

5. A gradient PCR instrument for easy maintenance and repair according to claim 1, characterized in that, Each of the two insertion rods (3) is fitted with a tension spring (4) at one end, and one end of the insertion rod (3) is elastically connected to the outer wall of the gradient PCR instrument body (1) through the tension spring (4).

6. A gradient PCR instrument for easy maintenance and repair according to claim 1, characterized in that, The sample frame (5) has handle grooves (6) on both sides.

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