PCR (Polymerase Chain Reaction) micro-fluidic chip

Abstract

The utility model discloses a PCR (Polymerase Chain Reaction) micro-fluidic chip, which belongs to the technical field of biological detection and comprises a chip body and a shell, the chip body is placed in the shell, a cover plate is fixedly connected outside the shell through a hinge, buffer devices used for protecting the chip body are fixedly connected in the cover plate and the shell, and the buffer devices are fixedly connected in the shell. And the shell and the cover plate are externally and fixedly connected with the same locking device for limiting the shell and the cover plate. According to the utility model, when the chip body needs to be transferred, the chip body is firstly placed in the shell, then the cover plate is fixed on the shell through the hinge, at the moment, the locking rod is pushed to slide along the sliding sleeve, the locking rod can be accurately clamped in the locking sleeve, the cover plate is ensured to be stably closed, and when the outside is impacted or vibrated, the chip body can be conveniently transferred. When the chip body is damaged, a buffer spring in the internal buffer system can apply a counter-acting force to a buffer plate, so that external force transmission is effectively slowed down, the stability of the chip body in the shell is kept, and the chip is prevented from being damaged unnecessarily.

Description

TECHNICAL FIELD

[0001] The utility model belongs to biological detection technical field especially relates to a PCR micro -fluidic chip. BACKGROUND

[0002] Micro -fluidic chip transplant the basic operation unit such as sample preparation, mixing, reaction, separation, detection, and cell culture, sorting, lysis etc. involved in the field of chemistry or biology to on a small chip, and construct the micro -channel network throughout the whole chip, micro -fluidic chip can be as biological, chemical micro -reactor or micro -system. Nucleic acid detection has the characteristics of high sensitivity, specificity, in life science, medical examination, occupies the extremely important position, combines micro -fluidic chip technology and nucleic acid detection technology, develops the micro -fluidic PCR chip for biological sample high -throughput, full -automatic nucleic acid analysis detection, realizes reagent consumption small, analysis speed is fast, detection throughput is big, automation degree is high, non -professional personnel use etc. Advantage, especially suitable for the rapid quantitative detection of nucleic acid molecules in biological samples.

[0003] In the actual implementation process, the existing chip may be affected by external impact or improper operation during transfer or transportation, resulting in damage to the internal structure of the chip. This external force may cause the rupture, blockage or deformation of the micro -fluidic channel, reaction chamber or other delicate structure, thereby affecting the normal function and performance stability of the chip.

[0004] Based on this, the utility model discloses a PCR micro -fluidic chip to solve the above -mentioned problems. INVENTION CONTENTS

[0005] The utility model discloses a PCR micro -fluidic chip to solve the above -mentioned problems.

[0006] In order to realize the above -mentioned purpose, the utility model discloses a technical scheme as follows:

[0007] A PCR micro -fluidic chip, including chip body and shell, the chip body is placed in the shell, the shell is fixedly connected with the cover plate through the hinge outside, the cover plate and the shell are all fixedly connected with the buffer device for the chip body protection, the shell and the cover plate are fixedly connected with the same locking device for the both limit, the buffer device is fixedly connected with the adsorption device for the chip body pull up.

[0008] Further describe the above technical scheme:

[0009] The buffer device comprises four buffer springs fixedly connected to the buffer plates, wherein four of the buffer springs are fixedly connected in the shell, and the other four buffer springs are fixedly connected in the cover plate.

[0010] As a further description of the above technical solution:

[0011] The locking device comprises a connecting plate and a fixed plate, the connecting plate is fixedly connected to the cover plate, the fixed plate is fixedly connected to the front face of the shell, a sliding groove is formed in the connecting plate, a sliding sleeve is fixedly connected in the sliding groove, a locking rod is arranged in the sliding sleeve, and a locking sleeve is fixedly connected to the front face of the fixed plate.

[0012] As a further description of the above technical solution:

[0013] The locking rod and the sliding sleeve are in sliding connection, and the locking rod is located opposite to the locking sleeve.

[0014] As a further description of the above technical solution:

[0015] The size of the end face of the locking rod is slightly smaller than the internal size of the locking sleeve.

[0016] As a further description of the above technical solution:

[0017] The adsorption device comprises a circular plate, the circular plate is fixedly connected to the front face of one of the buffer plates, and a suction disc is fixedly connected to the front face of the circular plate.

[0018] As a further description of the above technical solution:

[0019] A through hole is formed in the suction disc, an air nozzle is fixedly connected in the through hole, and a micro pneumatic valve is arranged in the air nozzle.

[0020] As a further description of the above technical solution:

[0021] The micro pneumatic valve comprises a storage battery, a control circuit, a pneumatic actuator and a valve body.

[0022] As a result of the adoption of the above technical solution, the present application has the following advantages:

[0023] 1. The utility model discloses when needing to transfer the chip body, first, the chip body is placed into the shell inside, subsequently through the hinge fixed cover plate on the shell, at this time, push the locking rod along the sliding sleeve, and the locking rod will accurately be clamped into the locking sleeve, and the cover plate is firmly closed, when the outside is impacted or vibrated, the buffer spring in the internal buffer system can apply the reaction force to the buffer plate, thereby effectively alleviating the external force transmission, the stability of the chip body in the shell is kept, and the chip body is prevented from unnecessary damage.

[0024] 2. The utility model discloses when needing to open the cover plate, the sucking disc will first adsorb the chip body, then, through the control micro pneumatic valve, the airflow is released from the through hole through the air nozzle, thereby making the separation between the sucking disc and the chip body, and ensuring that the chip body can be smoothly and safely taken out or subsequent operation is carried out. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 It is a three-dimensional structure schematic view of the PCR microfluidic chip of the utility model;

[0026] Figure 2 It is a three-dimensional sectional structure schematic view of the buffer plate of the PCR microfluidic chip of the utility model;

[0027] Figure 3 It is a three-dimensional sectional structure schematic view of the buffer plate of the PCR microfluidic chip of the utility model; Figure 2

[0028] Figure 4 It is a three-dimensional sectional structure schematic view of the buffer plate of the PCR microfluidic chip of the utility model; Figure 2

[0029] Figure 5 It is a three-dimensional structure schematic view of the PCR microfluidic chip sucking device of the utility model;

[0030] LEGEND:

[0031] 1, chip body, 2, shell, 3, cover plate, 4, buffer device, 41, buffer plate, 42, buffer spring, 5, locking device, 51, connecting plate, 52, sliding slot, 53, sliding sleeve, 54, locking rod, 55, fixed plate, 56, locking sleeve, 6, sucking device, 61, round plate, 62, sucking disc, 63, through hole, 64, air nozzle, 65, micro pneumatic valve. DETAILED DESCRIPTION

[0032] ​​The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of the utility model.

[0033] Please refer to Figures 1-5 ;

[0034] First embodiment:

[0035] The utility model provides a technical scheme: a kind of PCR microfluidic chip, including chip body 1 and shell 2, chip body 1 is placed in shell 2, shell 2 is fixedly connected with cover plate 3 outside by hinge, cover plate 3 and shell 2 are all fixedly connected with the buffer device 4 for protecting chip body 1, shell 2 and cover plate 3 are fixedly connected with the same locking device 5 for limiting both, buffer device 4 is fixedly connected with the adsorption device 6 for pulling up chip body 1 in.

[0036] Specifically, as shown in Figures 2-4 Buffer plate 41, four buffer springs 42 are fixedly connected below buffer plate 41, buffer spring 42 will be compressed when subjected to external force, and the transmission of impact force is reduced, thereby reducing the damage to chip body 1;

[0037] Four buffer springs 42 are fixedly connected in shell 2, and the other four buffer springs 42 are fixedly connected in cover plate 3, locking device 5 includes connecting plate 51 and fixed plate 55, connecting plate 51 is fixedly connected on cover plate 3, fixed plate 55 is fixedly connected on the front of shell 2, sliding slot 52 is formed in connecting plate 51, sliding sleeve 53 is fixedly connected in sliding slot 52, locking rod 54 is arranged in sliding sleeve 53, the sliding fit of sliding sleeve 53 and locking rod 54 can effectively realize the stable closing and opening of cover plate 3, the accurate sliding fit between locking rod 54 in sliding sleeve 53 and locking sleeve 56 enables locking device 5 to easily complete the fixation and release of cover plate 3, without causing loosening or instability between chip body 1 and shell 2 due to misoperation or external force, which improves the sealing property of chip shell 2, ensures that cover plate 3 can be reliably closed when external conditions change, and is conveniently and quickly opened when needed, facilitating the removal and replacement of chip body 1;

[0038] The front of fixed plate 55 is fixedly connected with locking sleeve 56, locking rod 54 and sliding sleeve 53 form sliding connection fit, locking rod 54 and locking sleeve 56 are positionally corresponding, the size of the end face of locking rod 54 is slightly smaller than the internal size of locking sleeve 56.

[0039] During operation, when it is necessary to transfer the chip body 1, the chip body 1 is first placed inside the housing 2, and then the cover plate 3 is fixed to the housing 2 by the hinge. At this time, the locking rod 54 is pushed to slide along the sliding sleeve 53, and the locking rod 54 will accurately lock into the locking sleeve 56 to ensure that the cover plate 3 is securely closed. When the outside is subjected to impact or vibration, the buffer spring 42 in the internal buffer system will apply a reaction force to the buffer plate 41, thereby effectively reducing the transmission of external force, maintaining the stability of the chip body 1 inside the housing 2, and preventing the chip body 1 from being damaged unnecessarily.

[0040] Second embodiment:

[0041] Specifically, such as Figure 5 As shown, the adsorption device 6 includes a circular plate 61, which is fixedly connected to the front of one of the buffer plates 41. A suction cup 62 is fixedly connected to the front of the circular plate 61. A through hole 63 is opened in the suction cup 62. The through hole 63 inside the suction cup 62 cooperates with the air nozzle 64, so that the airflow can be precisely released through the through hole 63, thereby controlling the separation of the suction cup 62 from the chip body 1.

[0042] An air nozzle 64 is fixedly connected inside the through hole 63. A miniature pneumatic valve 65 is installed inside the air nozzle 64. The miniature pneumatic valve 65 includes a battery, a control circuit, a pneumatic actuator, and a valve body. The combination of the pneumatic valve and the miniature pneumatic control system makes the operation of the suction cup 62 more precise and efficient. The miniature pneumatic valve 65 precisely adjusts the pneumatic actuator through the control circuit to ensure that the suction cup 62 can adsorb the chip when needed and release it in time after the operation is completed. The battery inside the valve body provides sufficient power to ensure long-term stable operation of the system. Through this intelligent pneumatic control system, the automated processing of the chip body 1 can be better realized, reducing human operation errors and improving operation accuracy and efficiency.

[0043] When the cover plate 3 needs to be opened during operation, the suction cup 62 will first adsorb the chip body 1. Then, by controlling the micro pneumatic valve 65, the airflow is released from the through hole 63 through the air nozzle 64, thereby separating the suction cup 62 from the chip body 1, ensuring that the chip body 1 can be removed smoothly and safely or for subsequent operations.

[0044] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A PCR microfluidic chip comprising a chip body (1) and a housing (2), characterized in that, The chip body (1) is placed in the shell (2), the shell (2) is fixedly connected with the cover plate (3) outside through the hinge, the cover plate (3) and the shell (2) are fixedly connected with the buffer device (4) for protecting the chip body (1), the shell (2) and the cover plate (3) are fixedly connected with the same locking device (5) for limiting both, the buffer device (4) is fixedly connected with the adsorption device (6) for pulling up the chip body (1).

2. The PCR microfluidic chip according to claim 1, wherein, The buffer device (4) comprises a buffer plate (41), four buffer springs (42) are fixedly connected below the buffer plate (41), four of the buffer springs (42) are fixedly connected in the shell (2), and the other four buffer springs (42) are fixedly connected in the cover plate (3).

3. The PCR microfluidic chip according to claim 2, wherein, The locking device (5) comprises a connecting plate (51) and a fixed plate (55), the connecting plate (51) is fixedly connected on the cover plate (3), the fixed plate (55) is fixedly connected on the front face of the shell (2), a sliding groove (52) is formed in the connecting plate (51), a sliding sleeve (53) is fixedly connected in the sliding groove (52), a locking rod (54) is arranged in the sliding sleeve (53), and the front face of the fixed plate (55) is fixedly connected with a locking sleeve (56).

4. The PCR microfluidic chip according to claim 3, wherein, The locking rod (54) and the sliding sleeve (53) are in sliding connection, and the locking rod (54) corresponds in position to the locking sleeve (56).

5. The PCR microfluidic chip according to claim 4, wherein, The size of the end face of the locking rod (54) is slightly smaller than the internal size of the locking sleeve (56).

6. The PCR microfluidic chip according to claim 2, wherein, The adsorption device (6) comprises a circular plate (61), the circular plate (61) is fixedly connected to the front face of one of the buffer plates (41), and the front face of the circular plate (61) is fixedly connected with a suction disc (62).

7. The PCR microfluidic chip according to claim 6, wherein, A through hole (63) is formed in the suction disc (62), a gas nozzle (64) is fixedly connected in the through hole (63), and a micro pneumatic valve (65) is mounted in the gas nozzle (64).

8. The PCR microfluidic chip according to claim 7, wherein, The micro pneumatic valve (65) comprises a battery, a control circuit, a pneumatic actuator and a valve body.

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