Integrated stem cell waste liquid recovery and disinfection treatment device

Abstract

The utility model belongs to the field of cell waste liquid treatment, and particularly relates to an integrated stem cell waste liquid recovery and disinfection treatment device, which comprises a box body, a waste liquid collection bottle, an automatic batch feeder and a waste liquid cylinder, a plurality of compartments for placing equipment are arranged in the box body, the waste liquid collection bottle is arranged in the box body, and the automatic batch feeder is arranged in the box body. The automatic feeding machine is located on the upper portion of the waste liquid collecting bottle and fixedly connected to the inner wall of the box body, the waste liquid cylinder is arranged in a box body compartment different from the waste liquid collecting bottle, and the waste liquid cylinder and the automatic feeding machine are connected with the waste liquid collecting bottle through different pipelines. According to the device, the risk of cross contamination is reduced, the success rate of experiments is increased, radiation harm of ultraviolet rays to experimenters and cells is avoided, harm and influence of waste liquid to operators and experimental cells are reduced, and meanwhile environmental harm caused by pouring after the experiments are finished is avoided.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell waste liquid processing field, concretely relates to integrated stem cell waste liquid recovery, disinfection treatment device. BACKGROUND

[0002] When carrying out cell culture operation, a series of processes such as cell liquid change, digestion, cryopreservation etc. require extremely strict sterile environment, and the biological safety cabinet can provide a relatively isolated and clean space, which can effectively avoid the pollution of external microorganisms and guarantee the process of cell culture not to be disturbed by miscellaneous bacteria. But in actual operation, there is a problem that cannot be ignored, that is, cells will continuously consume the nutrients in the culture medium and produce metabolic waste, if the culture medium is not replaced or supplemented in time, the growth environment of cells will deteriorate, and then the normal growth, proliferation and physiological functions of cells will be affected. After the waste liquid in the waste liquid cylinder is full, the waste liquid needs to be taken out and poured, and then the waste liquid cylinder is disinfected and put into the biological safety cabinet again. In this process, there are certain risks, for example: when taking out the waste liquid, if the operation is improper, such as not sealing the container containing the waste liquid properly, the microorganisms in the waste liquid may escape and pollute the surrounding environment; after pouring the waste liquid, if the disinfection of the waste liquid cylinder is not thorough, the residual bacteria may be brought into the cell culture system with the new waste liquid when the waste liquid cylinder is used next time, thereby increasing the experimental risk, leading to the failure of cell culture and affecting the subsequent research work.

[0003] Disadvantages of the prior art:

[0004] Firstly, when operating in the biological safety cabinet, there is a strict requirement for sterile environment. However, whenever the waste liquid in the waste liquid cylinder reaches the upper limit, the waste liquid needs to be removed from the biological safety cabinet, poured and then disinfected. This seemingly simple operation process actually hides risks. In the process of removing the waste liquid, if the operator does not strictly perform the aseptic operation specification, such as when opening the waste liquid cylinder or transferring the waste liquid, a slight carelessness may let the bacteria from outside mix in. Once the bacteria enter the experimental environment in the biological safety cabinet, they are likely to contaminate the cell samples being cultured, thereby interfering with the experimental results, increasing the risk of experimental failure, and causing the large amount of time, effort and resources invested in the early stage to go to waste.

[0005] Secondly, when pouring the waste liquid each time, the waste liquid is splashed out. This phenomenon not only pollutes the surrounding experimental environment, making the originally clean experimental area contaminated with harmful substances in the waste liquid and interfering with the normal development of other experiments, but also, when the operation is slightly wrong, the waste liquid may be directly poured on the experimenter. This not only soils the clothes, but more seriously, the waste liquid may contain harmful substances such as chemical reagents and microorganisms, which, after contacting the skin, can cause health problems such as allergy and burn, greatly endangering the personal safety of the experimenter.

[0006] Therefore, in order to solve the above-mentioned problems, the integrated stem cell waste liquid recycling and disinfection treatment device is provided. Content of the utility model

[0007] The utility model discloses in view of the above-mentioned problems, and the integrated stem cell waste liquid recycling and disinfection treatment device is designed, the device reduces the cross contamination risk, improves the experimental success rate, and avoids the radiation harm of ultraviolet to the experimenter and cell, reduces the harm and influence of waste liquid to the operator and experimental cell, and avoids the environmental harm when pouring after the experiment is finished.

[0008] In order to realize the above-mentioned purpose, the utility model provides integrated stem cell waste liquid recycling and disinfection treatment device, including: box, waste liquid collection bottle, automatic feeder and waste liquid jar, the inside of the box is opened with a plurality of for placing equipment's compartment, the waste liquid collection bottle is placed in the box, the automatic feeder is located the upper portion of the waste liquid collection bottle and is fixedly connected on the inner wall of the box, the waste liquid jar is arranged in the different box compartment with the waste liquid collection bottle, the waste liquid jar and automatic feeder are connected with the waste liquid collection bottle through different pipelines.

[0009] Through the above-mentioned mode, the experimenter pours waste liquid into the waste liquid jar, and the waste liquid enters the waste liquid collection bottle through the pipeline, and the automatic feeder pours chemical disinfectant into the waste liquid collection bottle to disinfect the waste liquid.

[0010] Further, it further includes controller, the controller is arranged in the box, the box includes: inductive cover, switch group, glass window, infrared temperature sensor and ultraviolet disinfection lamp, the top of the box is provided with two independently openable inductive cover, the lower part of two inductive cover is the waste liquid collection bottle and waste liquid jar respectively, the switch group is arranged in the side of the box and is connected with the controller signal, the infrared temperature sensor, ultraviolet disinfection lamp and automatic feeder are connected with the controller signal, glass window is arranged on the side of the box, the infrared temperature sensor is arranged on the inductive cover of the upper portion of the waste liquid collection bottle, and the ultraviolet disinfection lamp is installed on the inner wall of the box and the inboard of inductive cover.

[0011] Through the above-mentioned mode, when needing to pour the waste liquid in the waste liquid collection bottle, first, the waste liquid and chemical disinfectant in the waste liquid collection bottle will react, and the reaction will heat, when the heat reaches a certain degree, it means that the waste liquid needs to be poured, the infrared temperature sensor on the top monitors the temperature to reach the standard, and transmits the signal to the controller, and the controller controls the inductive cover to open, so that the waste liquid can be poured.After pouring, the inductive cover is closed, and the controller controls the ultraviolet disinfection lamp to disinfect the waste liquid collection bottle.

[0012] Further, the top of the waste liquid collecting bottle is provided with a bottle cap, a first liquid inlet pipe and a second liquid inlet pipe are installed on the bottle cap, the first liquid inlet pipe is connected with the waste liquid cylinder, and the second liquid inlet pipe is connected with the automatic feeding machine.

[0013] Further, the top of the automatic feeding machine is provided with a feeding machine opening, a feeding machine cap is detachably connected on the feeding machine opening, and two arc-shaped positioning buckles are arranged on the inner wall of the box body and are clamped on the outer circle of the automatic feeding machine.

[0014] Further, the top of the waste liquid cylinder is provided with a waste liquid cylinder opening, a waste liquid cylinder cap that can be opened and closed is installed on the waste liquid cylinder opening, and an inclined circular baffle is arranged in the waste liquid cylinder.

[0015] Further, a first liquid discharge hose and a second liquid discharge hose are further arranged, one end of the first liquid discharge hose is connected with the bottom of the automatic feeding machine, the other end of the first liquid discharge hose is connected with the second liquid inlet pipe, one end of the second liquid discharge hose is connected with the bottom of the waste liquid cylinder, and the other end of the second liquid discharge hose is connected with the first liquid inlet pipe.

[0016] Further, a wire unwinding disc is further arranged, a bearing is arranged in the middle of the wire unwinding disc, and the outer circle of the wire unwinding disc is provided with the second liquid discharge hose.

[0017] Further, a base is arranged at the bottom of the waste liquid cylinder, the bottom of the base is of a concave structure, and the wire unwinding disc is arranged in the base.

[0018] In summary, the utility model has the following advantages and beneficial technical effects:

[0019] 1. The integrated stem cell waste liquid recycling and disinfection treatment device reduces the risk of cross contamination, improves the success rate of experiments, avoids the radiation hazards of ultraviolet rays on experimenters and cells, reduces the hazards and influences of waste liquid on operators and experimental cells, and avoids environmental hazards when pouring after experiments.

[0020] 2. The integrated stem cell waste liquid recycling and disinfection treatment device can reduce the possibility of waste liquid splashing outward when pouring, block the odor of waste liquid, and directly observe the water level of waste liquid in the inner cup through the observation window. BRIEF DESCRIPTION OF DRAWINGS

[0021] The above and / or additional aspects and advantages of the utility model will become apparent and more readily appreciated from the following description of the embodiments, with reference to the following drawings, in which:

[0022] Figure 1It is a structure schematic view of the integrated stem cell waste liquid recycling and disinfection treatment device of the utility model;

[0023] Figure 2 It is an internal structure schematic view of the integrated stem cell waste liquid recycling and disinfection treatment device of the utility model;

[0024] Figure 3 It is a running principle schematic view of the integrated stem cell waste liquid recycling and disinfection treatment device of the utility model.

[0025] Figure 4 It is a structure schematic view of the waste liquid cylinder cover of the integrated stem cell waste liquid recycling and disinfection treatment device of the utility model

[0026] The reference signs in the drawings are:

[0027] 01-box body;02-induction cover;03-switch group;04-glass window;05-infrared temperature sensor;06-ultraviolet disinfection lamp;07-waste liquid collection bottle;08-bottle cap;09-first liquid inlet pipe;10-second liquid inlet pipe;11-automatic feeding machine;12-positioning buckle;13-feeding machine cover;14-feeding machine port;15-first liquid discharge hose;16-second liquid discharge hose;17-base;18-winding disc;19-bearing;20-waste liquid cylinder;21-waste liquid cylinder cover;22-waste liquid cylinder port;23-rotation part;24-block cover;25-circular baffle;26-controller. DETAILED DESCRIPTION

[0028] The following will be described in detail with reference to the accompanying drawings Figures 1-4 The utility model is further explained in detail, and the example of the embodiment is shown in the drawings, wherein the same or similar reference signs represent the same or similar parts or the parts with the same or similar functions throughout. The embodiment described below with reference to the drawings is exemplary and is intended to explain the utility model and cannot be understood as a limitation of the utility model.

[0029] In the description of the utility model, unless otherwise explicitly specified and limited, the terms "connected", "connected", "fixed" should be understood broadly, for example, it can be fixed connection, can be detachable connection, can be mechanical connection, can be electrical connection, can be direct connection, can be indirect connection through intermediate medium, can be the communication inside two elements or the interaction relationship of two elements. For ordinary skilled in the art, the specific meaning of the above terms in the utility model can be understood according to the specific circumstances.

[0030] In the description of this embodiment, the terms "upper," "lower," "right," etc., refer to the orientation or positional relationship shown in the accompanying drawings. They are used solely for ease of description and simplification of operation, and do not indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first" and "second" are merely used for descriptive distinction and have no special meaning. All parts and equipment use conventional models found in the prior art, and the circuit connections employ conventional connection methods found in the prior art, which will not be detailed here. Content not described in detail in this specification belongs to prior art known to those skilled in the art.

[0031] like Figure 1 , Figure 2 and Figure 3 As shown, it includes: a box 01, a waste liquid collection bottle 07, an automatic feeder 11, and a waste liquid cylinder 20. The box 01 has several compartments for placing equipment. The waste liquid collection bottle 07 is placed in a compartment inside the box 01. The automatic feeder 11 is located above the waste liquid collection bottle 07 and is fixedly connected to the inner wall of the box 01. The waste liquid cylinder 20 is set in a compartment of the box 01 that is different from the waste liquid collection bottle 07. The waste liquid cylinder 20 and the automatic feeder 11 are both connected to the waste liquid collection bottle 07 through different pipes.

[0032] like Figure 1 , Figure 2 and Figure 3 As shown, it also includes a controller 26, which is located inside a compartment within the housing 01. The housing 01 includes: a sensor cover 02, a switch group 03, a glass window 04, an infrared temperature sensor 05, and an ultraviolet disinfection lamp 06. The top of the housing 01 has two independently openable sensor covers 02. The lower parts of the two sensor covers 02 are the compartments where the waste liquid collection bottle 07 and the waste liquid cylinder 20 are located, respectively. The switch group 03 is located on the side of the housing 01 and is signal-connected to the controller 26. The infrared temperature sensor 05 and the ultraviolet disinfection lamp 06... Both the automatic feeder 11 and the controller 26 are connected to the controller via signal. The glass window 04 is located on the side of the housing 01. The liquid level of the waste liquid collection bottle 07 can be observed through the glass window 04. The infrared temperature sensor 05 is located on the sensing cover 02 on the top of the waste liquid collection bottle 07. The sensing cover 02 has an installation hole. The infrared temperature sensor 05 is installed in the hole and can detect the internal temperature. The temperature of the waste liquid collection bottle 07 can be measured through the sensing cover 02. The ultraviolet disinfection lamp 06 is installed on the inner wall of the housing 01 and the inner side of the sensing cover 02.

[0033] like Figure 1 , Figure 2 and Figure 3As shown, the top of the waste liquid collection bottle 07 is provided with a bottle cap 08, the first liquid inlet pipe 09 and the second liquid inlet pipe 10 are installed on the bottle cap 08, the first liquid inlet pipe 09 is connected with the waste liquid cylinder 20, and the second liquid inlet pipe 10 is connected with the automatic feeding machine 11.

[0034] As shown in Figure 1 , Figure 2 and Figure 3 , the top of the automatic feeding machine 11 is provided with a feeding machine opening 14, the feeding machine cover 13 is detachably connected to the feeding machine opening 14, and the inner wall of the box body 01 is provided with two arc-shaped positioning buckles 12 in a bolted manner, the positioning buckles 12 are clamped on the outer circle of the automatic feeding machine 11, so that the automatic feeding machine 11 is fixed.

[0035] As shown in Figure 1 , the top of the waste liquid cylinder 20 is provided with a waste liquid cylinder opening 22, the waste liquid cylinder opening 22 is provided with a waste liquid cylinder cover 21 which can be opened and closed, the inside of the waste liquid cylinder 20 is provided with an inclined circular baffle 25, the waste liquid cylinder cover 21 is a semicircular cover structure, and an annular rotating part 23 is rotatably connected to the outer circle of the waste liquid cylinder cover 21, a semicircular cover 24 is integrally formed on the inner circle of the rotating part 23, the cover 24 can rotate with the rotating part 23, and the relative position of the cover 24 and the waste liquid cylinder cover 21 can be adjusted by rotating, and when the cover 24 completely covers the waste liquid cylinder cover 21, it is completely opened. On the contrary, it is completely closed.

[0036] As shown in Figure 2 , Figure 3 and Figure 4 Figure 1 Figure 2 Figure 3 , it also includes a first liquid discharge hose 15 and a second liquid discharge hose 16, one end of the first liquid discharge hose 15 is connected with the bottom of the automatic feeding machine 11, the other end is connected with the second liquid inlet pipe 10, one end of the second liquid discharge hose 16 is connected with the bottom of the waste liquid cylinder 20, and the other end is connected with the first liquid inlet pipe 09.

[0037] It also includes a wire spool 18, the middle part of the wire spool 18 is provided with a bearing 19, and the outer circle of the wire spool 18 is provided with the second liquid discharge hose 16. The bottom of the waste liquid cylinder 20 is provided with a base 17, the bottom of the base 17 is recessed, and the wire spool 18 is placed in the base 17.

[0038] The working principle of the integrated stem cell waste liquid recycling and disinfection treatment device is as follows:

[0039] By opening the switch group 03, the induction cover 02 on the waste liquid cylinder 20 is opened, the waste liquid is introduced into the waste liquid cylinder 20, the liquid enters the waste liquid collection bottle 07 through the second liquid discharge hose 16, the automatic feeding machine 11 introduces the disinfectant into the waste liquid collection bottle 07, and the waste liquid is disinfected, then the induction cover 02 is closed, and the ultraviolet disinfection lamp 06 disinfects the waste liquid in the waste liquid collection bottle 07.

[0040] The above are preferred embodiments of the present application, and are not intended to limit the protection scope of the present application, so that: any equivalent changes made according to the structure, shape, principle of the present application should be covered within the protection scope of the present application.

Claims

1. An integrated stem cell waste fluid recovery and disinfection treatment device, characterized in that, It includes: The box (01), waste liquid collection bottle (07), automatic feeding machine (11) and waste liquid cylinder (20), the inside of the box (01) is provided with a plurality of compartments for placing equipment, the waste liquid collection bottle (07) is placed in the box (01), the automatic feeding machine (11) is located at the upper part of the waste liquid collection bottle (07) and is fixedly connected to the inner wall of the box (01), the waste liquid cylinder (20) is arranged in a different compartment of the box (01) from the waste liquid collection bottle (07), and the waste liquid cylinder (20) and the automatic feeding machine (11) are connected with the waste liquid collection bottle (07) through different pipelines.

2. The integrated stem cell waste fluid recovery, disinfection treatment device according to claim 1, characterized in that, It also includes a controller (26), which is arranged in the box (01), and the box (01) comprises: an induction cover (02), a switch group (03), a glass window (04), an infrared temperature sensor (05) and an ultraviolet disinfection lamp (06), two independently openable induction covers (02) are arranged on the top of the box (01), the lower parts of the two induction covers (02) are respectively the waste liquid collection bottle (07) and the waste liquid cylinder (20), the switch group (03) is arranged on the side of the box (01) and is signal connected with the controller (26), the infrared temperature sensor (05), the ultraviolet disinfection lamp (06) and the automatic feeding machine (11) are signal connected with the controller (26), the glass window (04) is arranged on the side of the box (01), the infrared temperature sensor (05) is arranged on the induction cover (02) at the upper part of the waste liquid collection bottle (07), and the ultraviolet disinfection lamp (06) is installed on the inner wall of the box (01) and the inner side of the induction cover (02).

3. The integrated stem cell waste fluid recovery, disinfection treatment device according to claim 1, characterized in that, The top of the waste liquid collection bottle (07) is provided with a bottle cap (08), a first liquid inlet pipe (09) and a second liquid inlet pipe (10) are installed on the bottle cap (08), the first liquid inlet pipe (09) is connected with the waste liquid cylinder (20), and the second liquid inlet pipe (10) is connected with the automatic feeding machine (11).

4. The integrated stem cell waste fluid recovery, disinfection treatment device according to claim 1, characterized in that, The top of the automatic feeding machine (11) is provided with a feeding machine opening (14), a feeding machine cover (13) is detachably connected to the feeding machine opening (14), two arc-shaped positioning buckles (12) are arranged on the inner wall of the box (01), and the positioning buckles (12) are clamped to the outer circle of the automatic feeding machine (11).

5. The integrated stem cell waste fluid recovery, disinfection treatment device according to claim 1, characterized in that, The top of the waste liquid cylinder (20) is provided with a waste liquid cylinder opening (22), a waste liquid cylinder cover (21) which can be opened and closed is installed on the waste liquid cylinder opening (22), and an inclined circular baffle (25) is installed in the waste liquid cylinder (20).

6. The integrated stem cell waste fluid recovery, disinfection treatment device according to claim 3, characterized in that, It also includes a first liquid discharge hose (15) and a second liquid discharge hose (16), one end of the first liquid discharge hose (15) is connected with the bottom of the automatic feeding machine (11), the other end is connected with the second liquid inlet pipe (10), one end of the second liquid discharge hose (16) is connected with the bottom of the waste liquid cylinder (20), and the other end is connected with the first liquid inlet pipe (09).

7. The integrated stem cell waste fluid recovery, disinfection treatment device according to claim 6, characterized in that, Also include the pay-off reel (18), the middle part of the pay-off reel (18) is provided with bearing (19), the outer ring of the pay-off reel (18) is provided with the second drainage hose (16).

8. The integrated stem cell waste fluid recovery, disinfection treatment device according to claim 7, characterized in that, The bottom of the waste liquid cylinder (20) is provided with a base (17), and the bottom of the base (17) is concave, and the pay-off reel (18) is arranged in the base (17).

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