Organ-like tissue crushing device for test tube and sample temperature control accessory thereof

Abstract

The utility model provides an organ-like tissue breaking device for test tube and sample temperature control accessory thereof, relates to experimental equipment technical field, including protection mechanism, breaking mechanism, locking mechanism and liquid extraction mechanism, breaking mechanism and locking mechanism both are installed in protection mechanism, locking mechanism can push breaking mechanism to move, and the liquid extraction mechanism is located in the protection mechanism. The protection mechanism is arranged at one end of the crushing mechanism, the end of the crushing mechanism extends out of the interior of the protection mechanism and is limited, the liquid taking mechanism is arranged at the other end of the protection mechanism, the end of the liquid taking mechanism is connected with the end of the crushing mechanism in an inserted mode, the crushing mechanism comprises a variable frequency motor, a storage battery, a transmission rod and a crushing tool bit, and the variable frequency motor is electrically connected with the storage battery. During use, the variable frequency motor and the storage battery are respectively arranged in the pen-shaped protective shell to drive the transmission rod to drive the crushing tool bit to rotate to crush tissue cells, and the device is high in integration degree, small in size, capable of directly performing crushing operation in a test tube and capable of crushing a small amount of tissue.

Description

Technical Field

[0001] This utility model relates to the field of experimental equipment technology, and in particular to an organoid tissue fragmentation device for test tubes. Background Technology

[0002] One of the key advances in stem cell research over the past decade has been the development of organoid systems. These in vitro culture systems comprise a self-renewing population of stem cells capable of differentiating into multiple organ-specific cell types, possessing similar spatial organization to the corresponding organs, and reproducing some of their functions, thus providing a highly physiologically relevant system. Organoids can be generated from tissue samples containing adult stem cells, single adult stem cells, or through directed differentiation of pluripotent stem cells. Because some organoid model systems are characterized by the presence of a viable stem cell population, organoids can be significantly expanded. For example, a single progenitor cell can generate up to 1 x 10^6 organs within 5 to 6 weeks. ^6 The liver organoids provide researchers with a highly reliable and scalable platform for studying various organs.

[0003] When conducting tissue sample observation and analysis, the tissue samples need to be broken down for better observation. Existing tissue disruption devices are generally too large, making it impossible or inconvenient to perform tissue sample disruption in test tubes, and small tissue samples simply cannot be disrupted.

[0004] Therefore, this application proposes a test tube organoid tissue disruption device for enabling the disruption of tissue samples within a test tube. Utility Model Content

[0005] In view of the shortcomings of the prior art described above, the purpose of this utility model is to provide an organoid tissue fragmentation device for test tubes to solve the problems in the prior art.

[0006] To achieve the above and other related objectives, this utility model provides a test tube organoid tissue fragmentation device, comprising: a protection mechanism, a fragmentation mechanism, a height locking mechanism, and a liquid extraction mechanism;

[0007] Both the crushing mechanism and the height-locking mechanism are installed inside the protection mechanism. The height-locking mechanism can push the crushing mechanism to move, so that the end of the crushing mechanism extends out from the inside of the protection mechanism and is limited.

[0008] The liquid taking mechanism is located at the other end of the protection mechanism, and the end of the liquid taking mechanism is inserted into the end of the crushing mechanism.

[0009] The crushing mechanism includes a variable frequency motor, a battery, a transmission rod, and a crushing cutter head. The variable frequency motor and the battery are electrically connected. The transmission rod is connected to the rotor of the variable frequency motor, and the two ends of the transmission rod are located at the openings at both ends of the protection mechanism.

[0010] The height locking mechanism includes a reset elastic element and a locking component. The locking component can drive the variable frequency motor and the battery to move, so that the crushing head can extend and retract. The shaft of the locking component is penetrated by the transmission rod.

[0011] The reset elastic element is sleeved on the outer surface of the transmission rod, and the two ends of the reset elastic element overlap with the end of the frequency converter motor and the inner wall of the protection mechanism, respectively.

[0012] Preferably, the crushing cutter head is detachably mounted on the end of the transmission rod, and both the transmission rod and the shaft of the crushing cutter head are hollow;

[0013] The battery is equipped with a frequency converter switch and a charging port, both of which extend to the outer surface of the protection mechanism. The frequency converter switch can control the power supply and operating frequency of the frequency converter motor.

[0014] Preferably, the protective mechanism includes a protective housing, and the outer surface of the protective housing is provided with a telescopic groove;

[0015] The locking component includes: a rotating base, a fixed limiting cylinder, and a pressure cylinder, wherein the rotating base, the fixed limiting cylinder, and the pressure cylinder are nested together, and a push block is provided on the outer surface of the pressure cylinder, wherein the push block extends to the outer surface of the protection mechanism.

[0016] The frequency converter switch, charging port, and push block all pass through the telescopic slide groove and can all slide within the telescopic slide groove.

[0017] Preferably, the protective housing has a support step and a limiting groove inside, the variable frequency motor has a limiting slider on its outer surface, the limiting slider is slidably connected to the limiting groove, and one end of the reset elastic element overlaps with the support step.

[0018] Preferably, the outer surface of the protective shell is provided with a clamping member, and the clamping member is elastic.

[0019] Preferably, the liquid extraction mechanism includes a negative pressure chamber, a connector, a piston, and a spring-loaded component. The connector is fixed to one end of the negative pressure chamber and communicates with the interior of the negative pressure chamber. The piston extends into the interior of the negative pressure chamber and can slide and compress air inside the negative pressure chamber. The spring-loaded component is located inside the negative pressure chamber and can apply a spring-loaded force to the piston.

[0020] Preferably, the connector has a socket groove inside, the end of the transmission rod can be inserted into the socket groove, and the upper end of the transmission rod can slide axially along the inside of the socket groove.

[0021] Preferably, the piston component includes a piston end and a push handle, with the piston end located inside the negative pressure chamber and the push handle extending to the outer surface of the negative pressure chamber;

[0022] The push handle is provided with damping holes and scale marks, and the damping holes and scale marks are distributed along the extension direction of the push handle;

[0023] The negative pressure chamber is equipped with a damping element, which cooperates with the damping hole to indicate the movement stroke of the piston.

[0024] Preferably, the damping component includes a damping telescopic cylinder, a damping spring, and a damping rod. The damping spring is installed inside the damping telescopic cylinder and applies pressure to one end of the damping rod. The other end of the damping rod extends through to the outside of the damping telescopic cylinder. The damping telescopic cylinder can be inserted into a damping hole.

[0025] A temperature control accessory for test tube samples, the temperature control accessory can be used in conjunction with a crushing device, the temperature control accessory includes a support base and a test tube rack, the test tube rack is disposed above the support base, and the top of the test tube rack is provided with several test tube holes for fixing test tubes;

[0026] A heating plate is provided between the support base and the test tube rack. A power supply is provided inside the support base, which can supply power to the heating plate. A liquid chamber is provided inside the test tube rack, and a heat-conducting plate is provided at the bottom of the liquid chamber. The heating plate and the heat-conducting plate are in contact with each other.

[0027] The interior of the liquid chamber is filled with liquid.

[0028] Preferably, the support base is respectively provided with a control component, a display screen and a quick connector, the control component is electrically connected to the heating plate and the quick connector is electrically connected to the power supply;

[0029] The control components, display screen, and quick connectors are all waterproofed.

[0030] As described above, the organoid tissue disruption device for test tubes of this invention has the following beneficial effects:

[0031] 1. This utility model uses a variable frequency motor and a battery installed inside a pen-shaped protective shell to drive a transmission rod to rotate the crushing blade and crush tissue cells. It has a high degree of integration and a small size, and can be used to crush tissues directly in test tubes, achieving the effect of crushing small amounts of tissue.

[0032] 2. This utility model applies a rebound pressure to the frequency converter motor by setting a reset elastic element inside the protective shell, and sets a rotating base, a fixed limiting cylinder and a pressure cylinder inside the protective shell to limit the position of the frequency converter motor, so that the crushing head can extend and retract inside the protective shell, thereby ensuring that the crushing head remains clean and safe when not in use.

[0033] 3. This utility model achieves convenient addition of tissue fluid when breaking up sample tissue by installing a negative pressure chamber at the end of the protective shell, connecting the connector at the end of the negative pressure chamber to the transmission rod, and installing a sliding piston inside the negative pressure chamber for extracting and injecting liquid.

[0034] 4. This utility model provides a damping hole and scale markings on the surface of the piston component, and uses the damping component and damping hole to provide stroke indication. Thus, when extracting or injecting tissue fluid, the measurement can be determined according to the scale markings, and the measurement can be confirmed by the prompt sound and resistance of the damping component, achieving the effect of precise measurement control.

[0035] 5. This utility model sets up a support base and a test tube rack, and sets up a heating plate on the support base to heat the liquid inside the liquid chamber, thereby heating and keeping the sample tissue warm. When storing the sample, it can be placed in a low temperature chamber. The liquid in the liquid chamber can keep the sample at a low temperature for a certain period of time, thus achieving low temperature storage of sample tissue and control of heating to enhance cell activity.

[0036] Therefore, this utility model effectively overcomes the various shortcomings of the prior art and has high industrial application value. Attached Figure Description

[0037] Figure 1 The diagram shown is a structural schematic of the crushing device of this utility model.

[0038] Figure 2 The diagram shown is a cross-sectional view of the crushing device of this utility model.

[0039] Figure 3 The diagram shown is a structural schematic of the height-locking mechanism of the crushing device of this utility model.

[0040] Figure 4 The diagram shows the connection between the crushing mechanism and the liquid extraction mechanism of the crushing device of this utility model.

[0041] Figure 5 The diagram shown is a schematic diagram of the liquid extraction mechanism of the crushing device of this utility model.

[0042] Figure 6 The diagram shown is a cross-sectional view of the liquid extraction mechanism of the crushing device of this utility model.

[0043] Figure 7 This utility model is shown. Figure 5 A magnified schematic diagram of the structure at point A in the middle.

[0044] Figure 8 The diagram shown is a structural schematic of the temperature control accessory of this utility model.

[0045] Figure 9 The diagram shown is a structural assembly diagram of the temperature control accessory of this utility model.

[0046] Figure 10 The image shown is a cross-sectional view of the test tube rack structure of the temperature control accessory of this utility model.

[0047] Component designation explanation

[0048] 101. Protective outer shell; 102. Telescopic slide rail; 103. Support step; 104. Limiting groove; 105. Clamping component; 201. Variable frequency motor; 202. Battery; 203. Transmission rod; 204. Crusher head; 205. Variable frequency switch; 206. Charging port; 207. Limiting slider; 301. Reset elastic element; 302. Rotating base; 303. Fixed limiting cylinder; 304. Pressure cylinder; 305. Push block; 401. Negative pressure chamber; 402. Connector; 4021, Socket groove; 403, Piston component; 4031, Damping hole; 4032, Scale mark; 404, Spring-loaded component; 405, Damping component; 4051, Damping telescopic cylinder; 4052, Damping spring; 4053, Damping rod; 501, Support base; 502, Test tube rack; 503, Test tube cavity; 504, Heating plate; 505, Liquid chamber; 506, Heat-conducting plate; 507, Control components; 508, Display screen; 509, Quick connector. Detailed Implementation

[0049] The following specific embodiments illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification.

[0050] Please see Figures 1 to 10It should be understood that the structures, proportions, sizes, etc., illustrated in the accompanying drawings are merely for illustrative purposes to aid those skilled in the art and are not intended to limit the scope of this invention. Therefore, they have no substantial technical significance. Any modifications to the structure, changes in proportions, or adjustments to size, without affecting the effectiveness and purpose of this invention, should still fall within the scope of the disclosed technical content. Furthermore, the terms "upper," "lower," "left," "right," "middle," and "one" used in this specification are merely for clarity and not intended to limit the scope of this invention. Changes or adjustments to their relative relationships, without substantially altering the technical content, should also be considered within the scope of this invention.

[0051] like Figures 1-7 As shown, this utility model provides a test tube organoid tissue disruption device, comprising: a protective mechanism, a disruption mechanism, a locking mechanism, and a liquid extraction mechanism. Both the disruption mechanism and the locking mechanism are installed inside the protective mechanism. The locking mechanism can push the disruption mechanism to move, allowing its end to extend out from the interior of the protective mechanism and be limited, enabling the end of the disruption mechanism to extend into the test tube. This prevents contamination of the end of the disruption mechanism and avoids injury to the operator when the disruption function is not in use. To further enhance safety and cleanliness, a detachable cap can be configured on the protective mechanism to seal its end.

[0052] The liquid extraction mechanism is located at the other end of the protective mechanism, allowing for the addition of tissue fluid at any time during the tissue disruption process, thus improving operational convenience. Furthermore, the end of the liquid extraction mechanism is inserted into the end of the disruption mechanism to enhance the sealing of the connection, ensuring that the disruption and liquid extraction mechanisms remain connected even when the disruption mechanism extends or retracts. The connection also remains intact even when the axis of the disruption mechanism rotates.

[0053] The crushing mechanism includes a variable frequency motor 201, a battery 202, a transmission rod 203, and a crushing cutter head 204. The variable frequency motor 201 and the battery 202 are electrically connected, with the battery 202 supplying power to the motor 201. The transmission rod 203 is connected to the rotor of the variable frequency motor 201. When the motor 201 is energized, the transmission rod 203 rotates under the influence of magnetic force. Both ends of the transmission rod 203 are located at the openings at both ends of the protection mechanism; one end is used to mount the blades, and the other end is used to connect to the liquid extraction mechanism. To improve stability, the battery 202 is cylindrical, with its outer surface fitting against the inner wall of the protective casing 101. A through-hole is provided in the middle of the battery 202 to allow the transmission rod 203 to pass through.

[0054] The crushing head 204 is installed at the end of the transmission rod 203 and is detachable and replaceable. When the diameter and length of the crushing head 204 do not match the test tube, it can be adapted to different test tube models by replacing it with a crushing head 204 of different diameter and / or length, thus improving compatibility. Both the transmission rod 203 and the crushing head 204 have hollow shafts, allowing liquid to be drawn from either the crushing head 204 or the transmission rod 203 and stored inside the transmission rod 203 during liquid extraction. A small amount of liquid will remain inside the transmission rod 203 and will not enter the liquid extraction mechanism, preventing contamination. For cleaning, simply rinse the cavity in the transmission rod 203 repeatedly with purified water or disinfectant.

[0055] The battery 202 is equipped with a frequency converter switch 205 and a charging port 206. The frequency converter switch 205 controls the power supply from the battery 202 to the frequency converter motor 201. The frequency converter switch 205 also controls the energization and operating frequency of the frequency converter motor 201, achieving an adjustable crushing rate. The charging port 206 is used to connect a power cord to charge the battery 202. Both the frequency converter switch 205 and the charging port 206 extend to the outer surface of the protection mechanism and move with the battery 202.

[0056] The height-locking mechanism includes a reset elastic element 301 and a locking component. The locking component includes a rotating base 302, a fixed limiting cylinder 303, and a pressure cylinder 304. The rotating base 302, fixed limiting cylinder 303, and pressure cylinder 304 are interlocked, forming a structure similar to the height lock of a ballpoint pen refill. In this application, it is used to lock the height of the variable frequency motor 201, ensuring the crushing blade 204 is stably positioned outside the protective mechanism and in contact with the sample tissue inside the test tube. A push block 305 is provided on the outer surface of the pressure cylinder 304, extending to the outer surface of the protective mechanism. The push block 305 facilitates finger-push pressure on the rotating base 302, causing the rotating base 302 to rotate under the pressure of the inclined teeth. This, in conjunction with the fixed limiting cylinder 303, achieves a positioning function, and the fixed limiting cylinder 303 is fixed to the inner wall of the protective mechanism. Pushing the push block 305 can drive the variable frequency motor 201 and the battery 202 to move, thereby enabling the crushing head 204 to extend and retract. The shafts of the rotating base 302, the fixed limiting cylinder 303, and the pressure cylinder 304 are all penetrated by the transmission rod 203, thereby avoiding interference with the rotation of the crushing head 204 and allowing the crushing head 204 to be connected to the liquid extraction mechanism.

[0057] The reset elastic element 301 is sleeved on the outer surface of the transmission rod 203, and its two ends overlap with the end of the variable frequency motor 201 and the inner wall of the protection mechanism, respectively. Thus, when the variable frequency motor 201 loses its limit constraint, the reset elastic element 301 will push the variable frequency motor 201 back to its initial position to realize the recovery of the crushing head 204 and improve the safety and cleanliness of the crushing head 204.

[0058] In some embodiments, the protective mechanism of this utility model includes a protective shell 101, the overall shape of which is similar to a ballpoint pen for easy carrying and operation. A telescopic groove 102 is formed on the outer surface of the protective shell 101. The frequency converter switch 205, the charging port 206, and the push block 305 all pass through the telescopic groove 102 and can slide within it, thus preventing motion interference between the battery 202 and the pressure cylinder 304, and limiting the angle of the battery 202 and the pressure cylinder 304.

[0059] In some embodiments, the protective housing 101 of this invention has a supporting step 103 and a limiting groove 104 inside. A limiting slider 207 is provided on the outer surface of the variable frequency motor 201. The limiting slider 207 is slidably connected to the limiting groove 104, thereby limiting the installation angle of the variable frequency motor 201 and preventing the variable frequency motor 201 from rotating when the rotor rotates. One end of the reset elastic member 301 overlaps with the supporting step 103 to support the reset elastic member 301, ensuring that the reset elastic member 301 can be stably positioned inside the protective housing 101 and preventing uneven force on the reset elastic member 301 from causing twisting deformation.

[0060] In some embodiments, the outer surface of the protective shell 101 of this utility model is provided with a clamping member 105, which is elastic. When carrying, the clamping member 105 can be clamped in a clothing pocket due to its elasticity, achieving the purpose of convenient carrying and not easily lost.

[0061] In some embodiments, the liquid extraction mechanism of this invention includes a negative pressure chamber 401, a connector 402, a piston 403, and a rebound member 404. The connector 402 is fixed to one end of the negative pressure chamber 401 and communicates with the interior of the negative pressure chamber 401. The piston 403 extends into the interior of the negative pressure chamber 401 and can slide inside the negative pressure chamber 401 to compress air, thereby extracting tissue fluid from the container through the principle of negative pressure. The rebound member 404 is located inside the negative pressure chamber 401 and can apply a rebound force to the piston 403, thereby facilitating the return of the piston 403 to its initial position for the extraction and injection of tissue fluid.

[0062] In some embodiments, the connector 402 of this invention has a socket groove 4021 inside, and the end of the transmission rod 203 can be inserted into the socket groove 4021, thereby improving the sealing of the connection point through the insertion of the socket groove 4021 and the transmission rod 203. The upper end of the transmission rod 203 can slide axially along the inside of the socket groove 4021, and the socket groove 4021 and the transmission rod 203 always maintain an insertion relationship when the end of the transmission rod 203 is displaced. At the same time, the connection between the socket groove 4021 and the transmission rod 203 remains smooth to avoid affecting the rotation of the transmission rod 203.

[0063] In some embodiments, the piston component 403 of this invention includes a piston end and a push handle. The piston end is located inside the negative pressure chamber 401, and the push handle extends to the outer surface of the negative pressure chamber 401. The piston end is completely fitted with the inner wall of the negative pressure chamber 401 to achieve a seal, while the push handle is used to facilitate the movement of the piston end inside the negative pressure chamber 401 by a finger.

[0064] The push handle is provided with damping holes 4031 and scale marks 4032, both distributed along the extension direction of the push handle. A damping element 405 is provided inside the negative pressure chamber 401. The damping element 405 cooperates with the damping holes 4031 to indicate the movement stroke of the piston element 403. Specifically, the damping holes 4031 are spaced at intervals of 5ml, 10ml, or a customized spacing. Each time the damping hole 4031 contacts the damping element 405, the end of the damping element 405 engages inside the damping hole 4031 to create resistance, indicating the movement to the specified interval. Only by applying greater pressure can the piston continue to move beyond the damping holes 4031. At this time, the function of the spring element 404 is to prevent excessive pressure when increasing the applied force, thus preventing excessive movement of the piston element 403. Using a 5ml interval as an example, when the piston 403 is pressed, after experiencing two resistances, it is clear that 10ml of tissue fluid has been drawn or injected, and so on. There is no need to deliberately observe the graduation mark 4032, improving ease of use.

[0065] In some embodiments, the damping component 405 of this invention includes a damping telescopic cylinder 4051, a damping spring 4052, and a damping rod 4053. The damping spring 4052 is installed inside the damping telescopic cylinder 4051 and applies pressure to one end of the damping rod 4053. The other end of the damping rod 4053 extends through to the outside of the damping telescopic cylinder 4051, which can be inserted into the damping hole 4031. The damping spring 4052 ensures that the damping rod 4053 consistently applies pressure to the outer surface of the piston member 403's push handle, and that the damping rod 4053 is inserted into the damping hole 4031. A mechanical impact sound is generated the moment the damping rod 4053 is inserted into the damping hole 4031 to enhance the alerting effect. Both the end of the damping rod 4053 and the opening of the damping hole 4031 are chamfered, so that when the damping rod 4053 is subjected to greater pressure, it can retract into the damping telescopic cylinder 4051 and cross the damping hole 4031.

[0066] like Figures 8-10 As shown, a temperature control accessory for test tube samples includes a support base 501 and a test tube rack 502. The test tube rack 502 is positioned above the support base 501. The top of the test tube rack 502 has several test tube pockets 503 for securing the test tubes.

[0067] A heating plate 504 is disposed between the support base 501 and the test tube rack 502. The support base 501 contains a power supply that powers the heating plate 504, causing it to heat up. The heating plate 504 is a PTC semiconductor heating element used for controllable heating, allowing the heating temperature to be controlled as needed. The test tube rack 502 contains a liquid chamber 505 filled with liquid. Therefore, when the heating plate 504 heats, the liquid is heated first, and the liquid evenly diffuses the heat to heat the test tubes inserted in the test tube cavity 503, preventing uneven heating. Simultaneously, the outer surface of the test tube rack 502 is made of insulating material to keep the liquid warm and reduce heat loss. A heat-conducting plate 506 is disposed at the bottom of the liquid chamber 505, and the heating plate 504 contacts the heat-conducting plate 506. This improves the efficiency of heat conduction from the heating plate 504 to the liquid chamber 505. The heating temperature is generally controlled below 25 degrees Celsius to avoid inactivation due to excessive heat. When sample storage is required, the entire temperature control unit can be placed in a cold storage compartment for low-temperature preservation. After removing the temperature control unit, the liquid in the liquid chamber 505 has poor thermal conductivity, thus maintaining the sample at a low temperature for a period of time, similar to the effect of a laboratory ice tray.

[0068] In some embodiments, the support base 501 of this invention is respectively provided with a control component 507, a display screen 508, and a quick connector 509. The control component 507 is electrically connected to the heating plate 504, and controls the output power of the heating plate 504 through the control component 507, thereby achieving controllable heating temperature. The quick connector 509 is electrically connected to a power supply, and is used to charge and / or supply power to the power supply installed inside the support base 501 through a power cord. The power supply is generally a lithium battery, but it can also be a power module. The display screen 508 is used to display the temperature set by the control component 507, and to display the temperature of the liquid inside the liquid chamber 505. In order to monitor the temperature inside the liquid chamber 505, a temperature sensor is provided inside the liquid chamber 505.

[0069] The control unit 507, display screen 508, and quick connector 509 are all waterproofed. This is to prevent condensation from entering the support base 501 through the gaps between the control unit 507, display screen 508, and quick connector 509 when the temperature control components are placed in the low-temperature storage, which could cause a short circuit.

[0070] The specific usage process of this utility model is as follows:

[0071] By pushing the push block 305, the crushing head 204 extends into the interior of the protective housing 101;

[0072] Press the piston 403 and pull it back to extract an appropriate amount of tissue fluid under negative pressure for later use.

[0073] Insert the test tube containing the sample tissue into the test tube cavity 503 of the temperature control component to fix the test tube in place;

[0074] The crushing blade 204 extends into the interior of the test tube and comes into contact with the sample tissue;

[0075] The variable frequency motor 201 is started to drive the transmission rod 203 to rotate at high speed, and the sample tissue is crushed by the crushing cutter head 204;

[0076] The appropriate amount of tissue fluid is injected into the test tube by pressing the piston 403 at any time, so that the sample tissue remains fluid when it is broken.

[0077] Depending on the needs of the sample tissue, the heating plate 504 can be activated to heat the test tube as needed, or the temperature control accessories for low-temperature preservation can be used to enhance or reduce the activity of the sample tissue.

[0078] In summary, the organoid tissue disruption device for test tubes of this invention uses a variable frequency motor 201 and a battery 202 installed inside a pen-shaped protective shell 101 to drive a transmission rod 203 to rotate a disruption head 204 to disrupt tissue cells. It has a high degree of integration and a small size, and can perform disruption operations directly in test tubes, achieving the effect of disrupting small amounts of tissue.

[0079] By setting a reset elastic element 301 inside the protective housing 101 to apply rebound pressure to the frequency converter motor 201, and by setting a rotating base 302, a fixed limiting cylinder 303 and a pressure cylinder 304 inside the protective housing 101 to limit the position of the frequency converter motor 201, the crushing head 204 can extend and retract inside the protective housing 101, thereby ensuring that the crushing head 204 remains clean and safe when not in use.

[0080] By installing a negative pressure chamber 401 at the end of the protective shell 101, connecting the connector 402 at the end of the negative pressure chamber 401 to the transmission rod 203, and installing a sliding piston 403 inside the negative pressure chamber 401 for extracting and injecting liquid, it is possible to conveniently add tissue fluid when breaking up sample tissue.

[0081] By setting a damping hole 4031 and a scale mark 4032 on the surface of the piston 403, and by cooperating with the damping element 405 and the damping hole 4031 to provide stroke indication, the measurement can be judged according to the scale mark 4032 when extracting or injecting tissue fluid, and the measurement can be confirmed by the prompt sound and resistance of the damping element 405, thus achieving the effect of precise measurement control.

[0082] By setting up a support base 501 and a test tube rack 502 in cooperation, and setting a heating plate 504 on the support base 501 to heat the liquid inside the liquid chamber 505, the sample tissue is heated and kept warm. When storing the sample, it can be placed in a low temperature chamber. The liquid in the liquid chamber 505 can keep the sample at a low temperature for a certain period of time, thus achieving the control of low temperature storage and heating to enhance cell activity of the sample tissue.

[0083] Therefore, this utility model effectively overcomes the various shortcomings of the prior art and has high industrial application value.

[0084] The above embodiments are merely illustrative of the principles and effects of this utility model and are not intended to limit the scope of this utility model. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of this utility model. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in this utility model should still be covered by the claims of this utility model.

Claims

1. A device for disrupting organoid tissue in test tubes, characterized in that, include: Protection mechanism, crushing mechanism, height locking mechanism, and liquid extraction mechanism; Both the crushing mechanism and the height-locking mechanism are installed inside the protection mechanism. The height-locking mechanism can push the crushing mechanism to move, so that the end of the crushing mechanism extends out from the inside of the protection mechanism and is limited. The liquid taking mechanism is located at the other end of the protection mechanism, and the end of the liquid taking mechanism is inserted into the end of the crushing mechanism. The crushing mechanism includes a variable frequency motor (201), a storage battery (202), a transmission rod (203), and a crushing cutter head (204). The variable frequency motor (201) and the storage battery (202) are electrically connected. The transmission rod (203) is connected to the rotor of the variable frequency motor (201), and the two ends of the transmission rod (203) are respectively located at the openings at both ends of the protection mechanism. The height locking mechanism includes a reset elastic element (301) and a locking component. The locking component can drive the variable frequency motor (201) and the battery (202) to move, so that the crushing head (204) can extend and retract. The shaft of the locking component is penetrated by the transmission rod (203). The reset elastic element (301) is sleeved on the outer surface of the transmission rod (203), and the two ends of the reset elastic element (301) overlap with the end of the frequency converter motor (201) and the inner wall of the protection mechanism, respectively.

2. The organoid tissue disruption device for test tubes according to claim 1, characterized in that: The crushing cutter head (204) is detachably mounted on the end of the transmission rod (203), and the shafts of both the transmission rod (203) and the crushing cutter head (204) are hollow. The battery (202) is provided with a frequency converter switch (205) and a charging port (206). The frequency converter switch (205) and the charging port (206) both extend to the outer surface of the protection mechanism, and the frequency converter switch (205) can control the power supply and operating frequency of the frequency converter motor (201).

3. The organoid tissue disruption device for test tubes according to claim 2, characterized in that: The protective mechanism includes a protective shell (101), and the outer surface of the protective shell (101) is provided with a telescopic groove (102). The locking component includes a rotating base (302), a fixed limiting cylinder (303), and a pressure cylinder (304). The rotating base (302), the fixed limiting cylinder (303), and the pressure cylinder (304) are nested together. A push block (305) is provided on the outer surface of the pressure cylinder (304), and the push block (305) extends to the outer surface of the protection mechanism. The frequency converter switch (205), the charging port (206) and the push block (305) all pass through the telescopic slide (102) and can all slide in the telescopic slide (102).

4. The organoid tissue disruption device for test tubes according to claim 3, characterized in that: The protective housing (101) is provided with a support step (103) and a limiting groove (104) inside. The variable frequency motor (201) is provided with a limiting slider (207) on its outer surface. The limiting slider (207) is slidably connected to the limiting groove (104). One end of the reset elastic element (301) overlaps with the support step (103).

5. The organoid tissue disruption device for test tubes according to claim 3, characterized in that: The outer surface of the protective shell (101) is provided with a clamping member (105), which is elastic.

6. The organoid tissue disruption device for test tubes according to claim 1, characterized in that: The liquid extraction mechanism includes a negative pressure chamber (401), a connector (402), a piston (403), and a spring-loaded component (404). The connector (402) is fixed to one end of the negative pressure chamber (401) and communicates with the interior of the negative pressure chamber (401). The piston (403) extends into the interior of the negative pressure chamber (401) and can slide and compress air inside the negative pressure chamber (401). The spring-loaded component (404) is located inside the negative pressure chamber (401) and can apply a spring-loaded force to the piston (403).

7. The organoid tissue disruption device for test tubes according to claim 6, characterized in that: The connector (402) has a socket groove (4021) inside, and the end of the transmission rod (203) can be inserted into the socket groove (4021), and the upper end of the transmission rod (203) can slide along the internal axial direction of the socket groove (4021).

8. The organoid tissue disruption device for test tubes according to claim 6, characterized in that: The piston component (403) includes a piston end and a push handle. The piston end is located inside the negative pressure chamber (401), and the push handle extends to the outer surface of the negative pressure chamber (401). The push handle is provided with damping holes (4031) and scale marks (4032), and the damping holes (4031) and scale marks (4032) are distributed along the extension direction of the push handle; The negative pressure chamber (401) is provided with a damping element (405), which cooperates with the damping hole (4031) to guide the movement of the piston (403).

9. The organoid tissue disruption device for test tubes according to claim 8, characterized in that: The damping component (405) includes a damping telescopic cylinder (4051), a damping spring (4052), and a damping rod (4053). The damping spring (4052) is installed inside the damping telescopic cylinder (4051) and applies pressure to one end of the damping rod (4053). The other end of the damping rod (4053) extends through to the outside of the damping telescopic cylinder (4051). The damping telescopic cylinder (4051) can be inserted into the damping hole (4031).

10. A sample temperature control accessory for test tubes, characterized in that: The temperature control accessory can be used in conjunction with any one of the crushing devices as described in claims 1-9. The temperature control accessory includes a support base (501) and a test tube rack (502). The test tube rack (502) is disposed above the support base (501). The top of the test tube rack (502) is provided with a plurality of test tube holes (503) for fixing test tubes. A heating plate (504) is provided between the support base (501) and the test tube rack (502). A power supply is provided inside the support base (501) to supply power to the heating plate (504). A liquid chamber (505) is provided inside the test tube rack (502). A heat-conducting plate (506) is provided at the bottom of the liquid chamber (505). The heating plate (504) and the heat-conducting plate (506) are in contact with each other. The liquid chamber (505) is filled with liquid.

Images