Reaction chamber cover plate assembly of real-time fluorescence PCR analyzer

Abstract

The utility model relates to a reaction cabin cover plate assembly of a real-time fluorescence PCR analyzer, which comprises an upper shell, a cover plate and a reaction cabin upper shell, the reaction cabin upper shell is fixedly arranged on the inner side of a detection slot of the upper shell, sliding chutes are arranged on two sides of the top of the reaction cabin upper shell, an avoiding table is arranged between the sliding chutes on two sides, and the cover plate is arranged on the avoiding table. Sliding rails in sliding connection with the sliding grooves are arranged on the two sides of the bottom of the cover plate. According to the real-time fluorescent PCR analyzer, the cover plate is connected with the upper shell of the reaction cabin in a sliding manner, so that the top of the upper shell of the reaction cabin can be opened by sliding the cover plate, and a reaction tube for biological sample detection is further placed into the reaction cabin, the cover plate can be prevented from rebounding, the detection time is prevented from being delayed, the real-time fluorescent PCR analyzer is not easy to damage, and the service life of the analyzer is prolonged.

Description

Technical Field

[0001] This utility model relates to the field of PCR analyzer technology, and in particular to a reaction chamber cover assembly for a real-time fluorescence PCR analyzer. Background Technology

[0002] PCR analyzers consist of three basic reaction steps: denaturation, annealing, and extension. They have advantages such as high specificity, high sensitivity, simple operation, and time saving. They can be used not only for basic research such as gene isolation, cloning, and nucleic acid sequence analysis, but also for increasingly widespread application in the diagnosis of clinical diseases.

[0003] Traditional PCR analyzers use a flip-top structure to seal the reaction chamber. The cover needs to be flipped over for each test. Occasionally, excessive force may cause the cover to spring back. Furthermore, the flip-top may loosen or break after prolonged use, causing problems with the sealing of the chamber and rendering the instrument unusable. Utility Model Content

[0004] The purpose of this invention is to provide a reaction chamber cover assembly for a real-time fluorescence PCR analyzer to solve the problems encountered in the background art.

[0005] To achieve the above objectives, the technical solution of this utility model is as follows:

[0006] A reaction chamber cover assembly for a real-time fluorescence PCR analyzer includes an upper shell, a cover plate, and a reaction chamber upper shell. The reaction chamber upper shell is fixedly installed inside the detection slot of the upper shell. The top two sides of the reaction chamber upper shell are provided with sliding grooves, and an abutment is provided between the two sliding grooves. The bottom two sides of the cover plate are provided with sliding rails that are slidably connected to the sliding grooves.

[0007] In the above scheme, the height of the clearance platform is not less than the thickness of the main body of the cover plate, and the top of the cover plate is provided with a lever. After the upper shell of the reaction chamber and the upper shell are fixedly installed with screws, the lever is located in the detection slot.

[0008] In the above scheme, a detection and storage slot is provided on the inner top of the upper shell of the reaction chamber, and a reaction port is provided in the middle of the bottom of the detection and storage slot. As a preferred embodiment, a slot is provided on the outer side of the upper shell of the reaction chamber for engaging with the lower shell of the reaction chamber.

[0009] In the above scheme, an indicator light slot is provided on one side of the detection slot on the top of the upper housing, and countersunk holes are provided at the four ends of the top of the upper housing. A decorative frame is also provided on the outer top surface of the upper housing, and the indicator light slot and the detection slot are located in the decorative frame. As a preferred embodiment, through-hole posts are provided at the four ends of the inner side of the upper housing, and the positions of the through-hole posts correspond to the countersunk holes. Ribs are provided between the through-hole posts on both sides. A limiting block is provided on the inner side of the upper housing behind the detection slot, and a positioning block is provided on the inner side of the upper housing in front of the detection slot.

[0010] Compared with existing technologies, the advantages of this invention are as follows: Because the cover plate is slidably connected to the upper shell of the reaction chamber, when the user uses the real-time fluorescence PCR analyzer, the cover plate can be slid open to open the reaction chamber, the PCR tube for the test sample can be placed into the sample detection well, and the cover plate can be slid closed to close the reaction chamber for detection, thus blocking interference from external light. When not in use, sliding the cover plate to close the reaction chamber can prevent contamination of the interior of the reaction chamber by external dust and other contaminants. Compared with traditional PCR, this real-time fluorescence PCR analyzer avoids the phenomenon of cover plate springing back, avoids delaying detection time, and is less prone to damage, increasing the service life of the instrument. Attached Figure Description

[0011] The disclosure of this utility model is illustrated with reference to the accompanying drawings. It should be understood that the drawings are for illustrative purposes only and are not intended to limit the scope of protection of this utility model. In the drawings, the same reference numerals are used to refer to the same parts. Wherein:

[0012] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0013] Figure 2 This is a schematic diagram of the exploded structure of this utility model;

[0014] Figure 3 This is a schematic diagram of the external structure of the upper shell in this utility model;

[0015] Figure 4 This is a schematic diagram of the inner structure of the upper shell in this utility model;

[0016] Figure 5 This is a schematic diagram of the structure of the cover plate and the upper shell of the reaction chamber in this utility model.

[0017] The following numbers are used in the diagram: 1-Upper shell; 11-Detection slot; 12-Indicator light slot; 13-Decorative frame; 14-Counterhead; 15-Through hole column; 16-Firming plate; 17-Limiting block; 18-Positioning block; 2-Cover plate; 21-Slide rail; 22-Handle; 3-Reaction chamber upper shell; 31-Detection insertion slot; 32-Slide groove; 33-Reaction insertion port; 34-Card slot; 35-Allowing platform. Detailed Implementation

[0018] To make the technical means, creative features, achieved objectives and effects of this utility model easier to understand, the utility model will now be described in further detail with reference to the accompanying drawings. These drawings are simplified schematic diagrams, illustrating only the basic structure of this utility model, and therefore only show the relevant components of this utility model.

[0019] Based on the technical solution of this utility model, without changing the essential spirit of this utility model, those skilled in the art can propose various interchangeable structural methods and implementation methods. Therefore, the following detailed embodiments and accompanying drawings are merely illustrative descriptions of the technical solution of this utility model, and should not be regarded as the entirety of this utility model or as a limitation or restriction of the technical solution of this utility model.

[0020] The technical solution of this utility model will be further described in detail below with reference to the accompanying drawings and embodiments.

[0021] like Figure 1 and Figure 2 As shown, a reaction chamber cover assembly for a real-time fluorescence PCR analyzer includes an upper shell 1, a cover 2, and a reaction chamber upper shell 3. The reaction chamber upper shell 3 is fixed to the reaction chamber lower shell during assembly and serves as a carrier for the biological detection reaction.

[0022] The upper shell 1 is installed directly above the real-time fluorescence PCR analyzer and serves as the inlet and outlet for biological detection. The upper shell 3 of the reaction chamber is fixedly installed inside the detection slot 11 of the upper shell 1. The top two sides of the upper shell 3 of the reaction chamber are provided with sliding grooves 32, and the two sliding grooves 32 are provided with a clearance platform 35 to avoid the space after the cover plate 2 is installed, so that the cover plate 2 can slide smoothly. The bottom two sides of the cover plate 2 are provided with slide rails 21 that are slidably connected to the sliding grooves 32.

[0023] During operation, because the cover plate 2 is slidably connected to the upper shell 3 of the reaction chamber, after the upper shell 3 of the reaction chamber is fixed to the inner side of the detection slot 11 of the upper shell 1, the top of the upper shell 3 of the reaction chamber can be opened by sliding the cover plate 2, and then the reaction tube for biological sample detection can be placed into the reaction chamber.

[0024] Specifically, when using the real-time fluorescence PCR analyzer, users can slide cover 2 to open the reaction chamber, place the PCR tube containing the sample into the sample detection well, and then slide cover 2 to close the reaction chamber for detection, thus blocking interference from external light. When not in use, sliding cover 2 to close the reaction chamber prevents contamination from external dust and other contaminants. Compared to traditional PCR, this real-time fluorescence PCR analyzer avoids the rebound phenomenon of cover 2, preventing delays in detection time and reducing the risk of damage, thus increasing the instrument's lifespan.

[0025] During implementation, the height of the clearance platform 35 is not less than the thickness of the main plate of the cover 2, so that the cover 2 can slide smoothly. To facilitate opening the reaction chamber, a lever 22 is provided on the top of the cover 2. The lever 22 has a protruding structure, and the reaction chamber can be easily opened by sliding it by pulling the lever 22. After the upper shell 3 of the reaction chamber is fixedly installed to the upper shell 1 with screws, the lever 22 is located in the detection slot 11, that is... Figure 1 The protrusion shown is in the detection slot 11, which facilitates opening.

[0026] Regarding the structural optimization of the upper shell 3 of the reaction chamber, please refer to [link / reference needed] for better biological detection experiments. Figure 5 The upper shell 3 of the reaction chamber has a detection storage slot 31 on its top inner side, which facilitates the insertion and removal of reaction tubes. A reaction port 33 is located at the bottom center of the detection storage slot 31, through which the reaction tubes are inserted into the reaction chamber. As a preferred embodiment, the outer wall of the upper shell 3 has a locking groove 34 that engages with the lower shell of the reaction chamber. This locking groove 34 secures the upper and lower parts of the reaction chamber together. Furthermore, during the assembly of the PCR analyzer, the left and right sides of the reaction chamber are fixed using sheet metal parts, providing a stable reaction environment for biological sample detection.

[0027] For structural optimization of the upper shell 1, please refer to [link / reference]. Figure 3 and Figure 4 An indicator light slot 12 is provided on one side of the detection slot 11 on the top of the upper shell 1. An indicator light for the detection reaction is installed inside the PCR analyzer. The indicator light is located inside the indicator light slot 12. When a biological sample is being detected, the reaction runs synchronously with the indicator light. The indicator light emits light from the indicator light slot 12, making it easy for the operator to identify that the PCR analyzer is detecting a sample.

[0028] A decorative frame 13 is provided on the outer top surface of the upper housing 1. The indicator light slot 12 and the detection slot 11 are located in the decorative frame 13. The decorative frame 13 has a racetrack-shaped structure, and its area is approximately the same as the area occupied by the cover plate 2 when it is closed and fully open. The top of the upper housing 1 has countersunk holes 14 at four ends to prevent the head of the long screw from protruding outward from the detection plane on the top of the upper housing 1 after screwing it in. The inner side of the upper housing 1 has through-hole posts 15 at four ends, which have the function of stabilizing the product structure and reinforcing it. The through-hole posts 15 are positioned corresponding to the countersunk holes 14, providing space for the long screw to be screwed in smoothly. The long screw extends from the countersunk hole 14, passes through the through-hole post 15, and is screwed onto the modular sheet metal bracket that fixes the reaction chamber inside. Ribs 16 are provided between the through-hole posts 15 on both sides. The connection of the ribs 16 makes the fixing structure of the upper housing 1 and the reaction chamber more solid and stable.

[0029] As a preferred embodiment, a limiting block 17 is provided on the inner side of the upper housing 1 behind the detection slot 11, and a positioning block 18 is provided on the inner side of the upper housing 1 in front of the detection slot 11. The limiting block 17 is used to limit the distance of the cover plate 2 sliding to the position of the maximum opening, and the positioning block 18 is the position of the cover plate 2 when it is closed, providing a positioning reference for the closed position.

[0030] The specific embodiments described above further illustrate the purpose, technical solution, and beneficial effects of this utility model. It should be understood that the above description is only a specific embodiment of this utility model and is not intended to limit the scope of protection of this utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the scope of protection of this utility model.

Claims

1. A reaction chamber cover assembly for a real-time fluorescence PCR analyzer, characterized in that: It includes an upper shell (1), a cover plate (2) and a reaction chamber upper shell (3). The reaction chamber upper shell (3) is fixedly installed inside the detection slot (11) of the upper shell (1). The top two sides of the reaction chamber upper shell (3) are provided with sliding grooves (32), and the two sides of the sliding grooves (32) are provided with a clearance platform (35). The bottom two sides of the cover plate (2) are provided with slide rails (21) that are slidably connected to the sliding grooves (32).

2. The reaction chamber cover assembly of a real-time fluorescence PCR analyzer according to claim 1, characterized in that: The height of the clearance platform (35) is not less than the thickness of the main body of the cover plate (2).

3. The reaction chamber cover assembly of a real-time fluorescence PCR analyzer according to claim 1, characterized in that: The top of the cover plate (2) is provided with a lever (22). After the upper shell (3) of the reaction chamber and the upper shell (1) are fixedly installed by screws, the lever (22) is located in the detection slot (11).

4. The reaction chamber cover assembly of a real-time fluorescence PCR analyzer according to claim 1, characterized in that: The upper shell (3) of the reaction chamber is provided with a detection and storage slot (31) on the inner side of the top, and a reaction port (33) is provided in the middle of the bottom of the detection and storage slot (31).

5. The reaction chamber cover assembly of a real-time fluorescence PCR analyzer according to claim 4, characterized in that: The outer wall of the upper shell (3) of the reaction chamber is provided with a slot (34) that engages with the lower shell of the reaction chamber.

6. The reaction chamber cover assembly of a real-time fluorescence PCR analyzer according to claim 1, characterized in that: An indicator light slot (12) is provided on one side of the detection slot (11) at the top of the upper housing (1), and countersunk holes (14) are provided at the four ends of the top of the upper housing (1).

7. The reaction chamber cover assembly of a real-time fluorescence PCR analyzer according to claim 6, characterized in that: The outer top surface of the upper housing (1) is also provided with a decorative frame (13), and the indicator light slot (12) and the detection slot (11) are located in the decorative frame (13).

8. The reaction chamber cover assembly of a real-time fluorescence PCR analyzer according to claim 6, characterized in that: The upper shell (1) has through-hole columns (15) at its four inner ends. The positions of the through-hole columns (15) and the countersunk holes (14) are corresponding. Ribs (16) are provided between the through-hole columns (15) on both sides.

9. The reaction chamber cover assembly of a real-time fluorescence PCR analyzer according to claim 6, characterized in that: The inner side of the upper housing (1) is provided with a limiting block (17) located behind the detection slot (11), and the inner side of the upper housing (1) is provided with a positioning block (18) located in front of the detection slot (11).

Images