Automatic sampling system and SPR analyzer

Abstract

The utility model provides an automatic sample introduction system and SPR analyser, the automatic sample introduction system includes sample bin, sampling needle, sampling tube, first drive mechanism, second drive mechanism and pump, sample bin includes movable disk and a plurality of sample containers, sample containers are connected with movable disk, the inlet end of sampling tube is connected with sampling needle, the inlet end of sampling tube is connected with the pump. The first driving mechanism can drive the movable disc to move so as to enable the sampling needle to be opposite to different sample containers, the second driving mechanism can drive the sampling needle to lift so as to enable the bottom end of the sampling needle to enter and exit from the sample containers, and the pump is mounted on the sampling pipe; the SPR analyzer comprises the automatic sampling system. According to the automatic sample injection system, automatic sample injection can be realized, the sample injection efficiency is improved, and the risk that a sample is influenced by misoperation during manual sample injection is reduced.

Description

Technical Field

[0001] This utility model relates to the field of biological detection technology, and in particular to an automatic sample introduction system and an SPR analyzer. Background Technology

[0002] In biological detection experiments, sample introduction into the detection equipment is usually done manually. The manual sampler is similar to a syringe; the user draws a certain amount of liquid sample into the sampler and then injects it into the corresponding detection device. Manual sample introduction is inefficient and prone to errors. For example, when multiple samples need to be injected into the detection device sequentially, the user needs to operate the manual sampler frequently, which can easily lead to user fatigue. This results in a decrease in the speed and accuracy of subsequent manual sampler operations, thus affecting the introduction efficiency and increasing the risk of errors. Utility Model Content

[0003] This invention aims to solve at least one of the technical problems existing in the prior art. To this end, this invention proposes an automatic sample injection system that can achieve automatic sample injection, overcoming the problems of low liquid injection efficiency and high error rate of manual sample injection.

[0004] This invention also proposes an SPR analyzer including the aforementioned automated sample introduction system. In this application, SPR refers to surface plasmon resonance.

[0005] An automatic sample introduction system according to a first aspect of the present invention includes: a sample compartment comprising a movable tray and a plurality of sample containers, the sample containers being connected to the movable tray and capable of containing samples; a sampling needle; a sampling tube comprising an inlet end and an outlet end, the inlet end being connected to the sampling needle; a first driving mechanism connected to the movable tray, the first driving mechanism being capable of driving the movable tray to move so that the sampling needle is positioned opposite to different sample containers; a second driving mechanism connected to the sampling needle, the second driving mechanism being capable of driving the sampling needle to rise and fall so that the bottom end of the sampling needle enters and exits the sample container; and a pump installed on the sampling tube, wherein, driven by the pump, the sample in the sample container positioned opposite to the sampling needle can enter the sampling tube through the sampling needle and flow to the outlet end of the sampling tube.

[0006] The automatic sample injection system according to the first aspect of the present invention has at least the following beneficial effects:

[0007] The second drive mechanism lowers the sampling needle, inserting it into the target container below the liquid level of the sample stored therein. Then, the pump starts, drawing the sample from the target container through the sampling needle into the sampling tube, which then flows to the outlet. The sample exiting the outlet can then be fed into a detection system or device for analysis. This achieves automated sample introduction. If further sample introduction is needed, the second drive mechanism raises the sampling needle. After the needle leaves the current target container, the first drive mechanism moves the movable disc, positioning another sample container below the sampling needle. Once the new sample container is below the needle, the needle can be reinserted, and the pump draws the sample from the container, driving it to flow to the outlet of the sampling tube. This process can be repeated multiple times to allow for further sample introduction.

[0008] In existing technologies, during manual sample addition using a syringe, the user needs to frequently insert and withdraw the syringe plunger, which can easily lead to user fatigue and errors. This fatigue can cause deviations in the plunger's movement speed and position, resulting in air bubbles in the sample, inaccurate sample volume, and other errors. The automatic sample addition system of this invention achieves automated sample addition, overcoming the problems of low efficiency and high error rate associated with manual sample addition, thus improving efficiency and reducing the risk of errors during the process.

[0009] According to some embodiments of this utility model, the pump is a peristaltic pump.

[0010] According to some embodiments of the present invention, the movable disk is circular, and a plurality of sample containers surround the center of the movable disk. The first driving mechanism includes a first motor, which is connected to the center of the movable disk and is capable of driving the movable disk to rotate.

[0011] According to some embodiments of the present invention, the second driving mechanism includes: a fixed base; a slider, which is fixedly connected to the sampling needle and slidably connected to the fixed base; a second motor, which is mounted on the fixed base; and a lead screw, which is connected to the second motor and vertically arranged. The lead screw is also threadedly connected to the slider. The second motor can drive the lead screw to rotate so that the slider rises and falls relative to the fixed base.

[0012] According to some embodiments of this utility model, the automatic sampling system further includes: a flow divider connected to the outlet end, the flow divider having multiple flow divider channels communicating with the lumen of the sampling tube; a flow cell, the flow cell being transparent and having multiple detection channels, the number of detection channels being the same as the number of flow divider channels; and connecting tubes, the number of connecting tubes being the same as the number of flow divider channels, the two ends of the connecting tubes being connected to the flow divider and the flow cell respectively, each connecting tube connecting one detection channel and one flow divider channel.

[0013] According to some embodiments of the present invention, the automatic sampling system further includes a flow cell, the flow cell is transparent, the flow cell is connected to the outlet end, the flow cell is provided with a detection channel, and the lumen of the sampling tube is connected to the detection channel.

[0014] According to a second aspect embodiment of the present invention, an SPR analyzer includes: a detection system and an automatic sample introduction system as described in the first aspect embodiment. The detection system includes: a prism, the prism being transparent, the side of the prism including a light-inlet surface, a light-outlet surface, and a detection surface connected end-to-end; an SPR sensor including a chip body and a gold film, the chip body being transparent and connected to the detection surface, the gold film being connected to a surface of the chip body facing away from the prism, and a flow cell being connected to a surface of the gold film facing away from the prism; a light source capable of emitting light, the light passing through the light-inlet surface into the prism and being reflected by the gold film before exiting from the light-outlet surface; and an image acquisition device disposed opposite to the light-outlet surface and capable of acquiring the light emitted from the light-outlet surface.

[0015] The SPR analyzer according to the first aspect of the present invention has at least the following beneficial effects:

[0016] According to some embodiments of the present invention, the SPR analyzer further includes: a housing having a chamber inside, wherein the detection system and the automatic sample injection system are both disposed in the chamber; and a temperature regulating device installed in the housing for regulating the temperature of the chamber.

[0017] According to some embodiments of the present invention, the temperature regulating device includes a constant temperature heating platform, the constant temperature heating platform includes a platform surface capable of generating heat, and at least a portion of the platform surface is located in the chamber.

[0018] According to some embodiments of the present invention, the SPR analyzer further includes a temperature sensor, which is installed in the housing and used to detect the temperature of the chamber. The temperature sensor is communicatively connected to the temperature regulating device, and the operating power of the temperature regulating device is adjusted according to the detection result of the temperature sensor.

[0019] Additional aspects and advantages of this invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0020] The present invention will be further described below with reference to the accompanying drawings and embodiments, wherein:

[0021] Figure 1 This is a schematic diagram of an automatic sample introduction system according to one embodiment of the present invention;

[0022] Figure 2 for Figure 1 A schematic diagram of the first drive mechanism and the sample chamber;

[0023] Figure 3 for Figure 1 A cross-sectional view of the diversion pool;

[0024] Figure 4 for Figure 1 A cross-sectional view of the flow cell;

[0025] Figure 5 This is a schematic diagram of an SPR analyzer according to one embodiment of the present invention;

[0026] Figure 6 for Figure 5 A schematic diagram of the internal structure of an SPR analyzer;

[0027] Figure 7 This is a schematic diagram of the detection system of the SPR analyzer of this utility model.

[0028] Figure label:

[0029] 101-Automatic sample introduction system, 102-First drive mechanism, 103-Sample chamber, 104-Sample container, 105-Moving disc, 106-Second drive mechanism, 107-Second motor, 108-Fixed base, 109-Outlet tube, 110-Lead screw, 111-Slider, 112-Sampling needle, 113-Inlet end, 114-Sampling tube, 115-Pump, 116-Outlet end, 117-Diverter, 118-Connecting tube, 119-Flow cell, 120-Diverter channel, 121-Detection channel;

[0030] 201-SPR analyzer, 202-box, 203-chamber, 204-constant temperature heating stage, 205-temperature sensor, 206-prism, 207-SPR sensor, 208-light source, 209-image acquisition device, 210-light-inlet surface, 211-light-outlet surface, 212-detection surface;

[0031] 301-Halogen lamp, 302-Liquid-core optical fiber, 303-First lens, 304-Acousto-optic tunable filter, 305-First cylindrical mirror, 306-Second cylindrical mirror, 307-Gold film, 308-Chip body, 309-Second lens, 310-Third lens;

[0032] 401 - Display screen, 402 - Fault indicator, 403 - Start button, 404 - Emergency stop button, 405 - Observation window, 406 - Host computer. Detailed Implementation

[0033] The embodiments of this utility model are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this utility model, and should not be construed as limiting this utility model.

[0034] In the description of this utility model, it should be understood that the directional descriptions, such as up, down, front, back, left, right, etc., indicate the directional or positional relationship based on the directional or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0035] In the description of this utility model, "several" means one or more, "multiple" means two or more, "greater than," "less than," and "exceeding" are understood to exclude the stated number, while "above," "below," and "within" are understood to include the stated number. The use of "first" and "second" in the description is merely for distinguishing technical features and should not be construed as indicating or implying relative importance, or implicitly indicating the number of indicated technical features, or implicitly indicating the order of the indicated technical features.

[0036] In the description of this utility model, unless otherwise explicitly defined, terms such as "setting," "installation," and "connection" should be interpreted broadly, and those skilled in the art can reasonably determine the specific meaning of the above terms in this utility model in conjunction with the specific content of the technical solution.

[0037] Figure 1An automatic sampling system 101 according to one embodiment of the present invention is shown. The automatic sampling system 101 includes a sample chamber 103, a sampling needle 112, a sampling tube 114, a first drive mechanism 102, a second drive mechanism 106, and a pump 115. The sample chamber 103 includes a movable disk 105 and multiple sample containers 104 for containing samples, which are liquids. The top of each sample container 104 has an opening for insertion of the sampling needle 112, and the sample container 104 can be configured as a test tube. The sample containers 104 are connected to the movable disk 105, and the connection can be made by the movable disk 105 having multiple mounting holes, with each sample container 104 inserted into one mounting hole. The sampling tube 114 includes an inlet end 113 and an outlet end 116, with the inlet end 113 connected to the sampling needle 112. The first drive mechanism 102 is connected to the movable disk 105, and can drive the movable disk 105 to move, thereby positioning the sampling needle 112 relative to different sample containers 104. For ease of description, the sample container 104 positioned relative to the sampling needle 112 will be referred to as the "target container" below. The second drive mechanism 106 is connected to the sampling needle 112, and can drive the sampling needle 112 to move up and down, thereby allowing the bottom end of the sampling needle 112 to enter and exit the target container. The pump 115 is installed in the sampling tube 114, and the pump 115 is used to drive the flow of the sample. The sampling tube 114 can be configured as a flexible tube.

[0038] The specific structures of the first drive mechanism 102 and the second drive mechanism 106 will be described below. The operation of the sample introduction system will be explained first. The operation of the sample introduction system is roughly as follows: First, the second drive mechanism 106 drives the sampling needle 112 to descend, inserting it into the target container and below the liquid surface of the sample stored in the target container. Then, the pump 115 starts, and driven by the pump 115, the sample in the target container enters the sampling tube 114 through the sampling needle 112, and then flows to the outlet end 116 of the sampling tube 114. The sample flowing out from the outlet end 116 of the sampling tube 114 can subsequently enter a detection system or device for sample analysis. This achieves automatic sample introduction.

[0039] If further sample injection is required, the second drive mechanism 106 drives the sampling needle 112 to rise. After the sampling needle 112 leaves the current target container, the first drive mechanism 102 drives the movable disk 105 to move, thereby positioning another sample container 104 below the sampling needle 112. After the new sample container 104 moves below the sampling needle 112, the sampling needle 112 can be inserted into the sample container 104 again. The pump 115 draws the sample from the sample container 104 and drives the sample flow to the outlet end 116 of the sampling tube 114. If multiple sample injections are required, the above steps can be repeated.

[0040] Therefore, the automatic sample injection system 101 of this utility model can realize automatic sample injection, save manpower, improve sample injection efficiency and reduce the risk of misoperation.

[0041] The automatic sample introduction system 101 will be further described below.

[0042] Pump 115 can be configured as a peristaltic pump, and correspondingly, sampling tube 114 is configured as a flexible tube. The pump head of the peristaltic pump is located outside the sampling tube 114, and drives the sample flow by squeezing the sampling tube 114. The advantage of using a peristaltic pump as pump 115 is that pump 115 does not come into contact with the sample inside the sampling tube 114, the sample is not contaminated by pump 115, and the accuracy of subsequent sample detection results is higher. In addition, during manual sample injection with a syringe, air bubbles are easily generated in the sample liquid when the user frequently pulls the syringe plunger; configuring pump 115 as a peristaltic pump helps reduce the air bubbles that appear in the sampling tube 114 during sample delivery, thereby improving the accuracy of sample detection results.

[0043] Reference Figure 2 , Figure 2 It shows Figure 1 The system includes a first drive mechanism 102 and a movable disk 105. The movable disk 105 is circular, and multiple sample containers 104 surround its center. The first drive mechanism 102 includes a first motor connected to the center of the movable disk 105. The first motor drives the movable disk 105 to rotate, thereby moving the different sample containers 104 directly below the sampling needle 112. Furthermore, the first motor can be configured as a servo motor, which helps to increase the movement speed and accuracy of the movable disk 105, allowing the sample containers 104 to move quickly and accurately below the sampling needle 112.

[0044] In other embodiments, the first drive mechanism 102 and the movable disk 105 may be configured in other ways. For example, the movable disk 105 may be rectangular, and the sample containers 104 mounted on the movable disk 105 may be arranged in a rectangular array. The first drive mechanism 102 may be configured as an XY displacement platform, driving the movable disk 105 to move along the X and Y axes on a horizontal plane, so that different sample containers 104 can be opposite to the sampling needle 112.

[0045] Relatively speaking, the advantage of setting the movable disk 105 as a circle and driving the movable disk 105 to rotate is that the space required for the movement of the movable disk 105 is smaller, the control method for the movement of the movable disk 105 is simpler, and the cost of the first drive mechanism 102 is lower.

[0046] Reference Figure 1The second drive mechanism 106 can be configured as a lead screw module, comprising a fixed base 108, a slider 111, a second motor 107, and a lead screw 110. In the vertical direction, the relative positions of the fixed bases 108 and 108 are fixed. The slider 111 is fixedly connected to the sampling needle 112 and also slidably connected to the fixed base 108, allowing it to slide up and down relative to the fixed base 108. The second motor 107 is connected to the fixed base 108, and the lead screw 110 is connected to the second motor 107. Driven by the second motor 107, the lead screw 110 can rotate around its own axis. The lead screw 110 passes through the slider 111, and its outer circumferential surface has threads (not shown), connecting it to the slider 111. Therefore, when the second motor 107 drives the lead screw 110 to rotate, the slider 111 can rise and fall relative to the fixed base 108, thereby causing the sampling needle 112 to rise and fall.

[0047] The advantage of setting the second drive mechanism 106 as a lead screw module is that it can improve the movement accuracy of the sampling needle 112 and facilitate the control of the position of the sampling needle 112. Furthermore, the second motor 107 can also be set as a servo motor to improve the movement speed and accuracy of the sampling needle 112. In addition, in some embodiments, the lead screw module can be replaced with other mechanisms capable of driving the linear movement of the sampling needle 112. For example, the second drive mechanism 106 can be set as a cylinder, electromagnet, linear motor, etc.

[0048] Figure 1 The automatic sampler shown also includes a split cell 117, a flow cell 119, a connecting tube 118, and an outlet tube 109. Accordingly, Figure 1 The automated sample introduction system 101 shown can be used with the SPR analyzer 201. Figure 3 This is a cross-sectional view of the diversion tank 117. Figure 4 This is a cross-sectional view of flow cell 119, and... Figure 3 It is intercepted by a horizontal plane. Figure 1 The cross-sectional view obtained from the diversion pool 117 in the middle. Figure 4 It is intercepted by a horizontal plane. Figure 1The cross-sectional view is obtained from the flow cell 119. The split cell 117 is connected to the outlet end 116 of the sampling tube 114. The split cell 117 is provided with multiple split channels 120, which communicate with the lumen of the sampling tube 114. The flow cell 119 is provided with multiple detection channels 121, the number of which is the same as the number of split channels 120. The number of connecting tubes 118 is also the same as the number of split channels 120. Both ends of the connecting tube 118 are connected to the split cell 117 and the flow cell 119, respectively, with each connecting tube 118 connecting one detection channel 121 and one split channel 120. In this way, the sample flowing out from the outlet end 116 of the sampling tube 114 can enter the split cell 117 and be split into the multiple split channels 120; subsequently, the sample in the split channels 120 can flow through the connecting tubes 118 to the detection channels 121 of the flow cell 119. Ultimately, each of the multiple detection channels 121 contains samples to be tested, and the samples in the flow cell 119 are subsequently detected and analyzed by the detection system of the SPR analyzer 201 (described below). The automated sample introduction system 101 of this embodiment is suitable for an SPR analyzer 201 with multi-channel detection capabilities. Combined with... Figure 1 and Figure 4 The inlet of the detection channel 121 is located at the top of the flow cell 119, and the outlet of the detection channel 121 is also located at the top of the flow cell 119. The outlet pipe 109 is connected to the outlet of the detection channel 121. After the test is completed, the sample can flow along the outlet pipe 109 to the container (not shown) for collecting waste liquid.

[0049] In some other embodiments, if the SPR analyzer 201 only requires single-channel detection, then the detection channel 121 in the flow cell 119 can be provided with only one channel; correspondingly, the automatic sample introduction system 101 can be without the split cell 117 and the connecting tube 118, the outlet end 116 of the sampling tube 114 is directly connected to the flow cell 119, the lumen of the sampling tube 114 is connected to the detection channel 121, and the sample flowing out from the outlet end 116 of the sampling tube 114 directly enters the flow cell 119.

[0050] It should be noted that when the automated sample introduction system 101 is applied to the SPR analyzer 201, the flow cell 119 needs to be transparent. For example, the flow cell 119 can be made of transparent materials such as PDMS (polydimethylsiloxane) or glass.

[0051] Figures 5 to 7 An SPR analyzer 201 according to one embodiment of the present invention is shown, which includes the aforementioned automated sample introduction system 101. (Refer to...) Figure 6The SPR analyzer 201 also includes a detection system, which comprises a prism 206, an SPR sensor 207, a light source 208, and an image acquisition device 209. The prism 206 is transparent, and its side surface includes a light-entry surface 210, a light-exit surface 211, and a detection surface 212 connected end-to-end. Figure 6 and Figure 7 In the prism 206, the light-incoming surface 210 is located on the lower left side, the light-outgoing surface 211 is located on the lower right side, and the detection surface 212 is located on the top of the prism 206. The SPR sensor 207 includes a chip body 308 and a gold film 307 stacked on top of each other (e.g., ...). Figure 7 (As shown). The chip body 308 is transparent; for example, it is made of glass. The chip body 308 is attached to the detection surface 212, and the gold film 307 is attached to the surface of the chip body 308 facing away from the prism 206, in combination. Figure 6 and Figure 7 The flow cell 119 is connected to the side surface of the gold film 307 facing away from the prism 206. That is, the chip body 308 is located above the detection surface 212, the gold film 307 is located above the chip body 308, and the flow cell 119 is located above the gold film 307. The light source 208 faces the light-inlet surface 210 and emits light. The light passes through the light-inlet surface 210 and enters the interior of the prism 206. After being reflected by the gold film, the light exits from the light-outlet surface 211. Figure 6 and Figure 7 In the diagram, the dashed path with arrows represents the path of light. The image acquisition device 209 includes a CCD camera, and is positioned opposite the light-emitting surface 211 and capable of acquiring light emitted from the light-emitting surface 211. For example... Figure 6 As shown, the SPR device also includes a host computer 406. The automatic sample introduction system 101 and the detection system are all connected to the host computer 406 for communication. The host computer 406 is used to control the operation of the automatic sample introduction system 101 and the detection system. The operation of the first drive mechanism 102, the second drive mechanism 106 and the pump 115 are all controlled by the host computer.

[0052] After the light passes through the SPR sensor 207, a portion of the light is absorbed, and the remaining light is emitted from the light-emitting surface 211. The image acquisition device 209 acquires the light and forms a spectral image, which is then analyzed by the host computer. The principle of analyzing samples using surface plasmon resonance is well-known in the field and will not be explained in detail here.

[0053] Reference Figure 7The light source 208 includes a halogen lamp 301 and a liquid-core fiber optic cable 302, and the light emitted directly from the light source 208 is white light. The detection system also includes a first lens 303, an acousto-optic tunable filter 304 (AOTF), a first cylindrical mirror 305, a second cylindrical mirror 306, a second lens 309, and a third lens 310 arranged sequentially along the light path. The light emitted from the light source 208 becomes a parallel beam after passing through the first lens 303. After passing through the AOTF, the light outputs P-polarized narrowband light. When the light passes through the first cylindrical mirror 305 and the second cylindrical mirror 306 and illuminates the gold film 307, it forms an elliptical spot to match the distortion of the coupling prism 206. After exiting from the light-emitting surface 211, the light is focused by the second lens 309 and the third lens 310 and then enters the image acquisition device 209.

[0054] Reference Figure 6 The SPR analyzer 201 also includes a housing 202 and a temperature control device. The housing 202 serves as the outer shell of the SPR analyzer 201, and its interior contains a chamber 203. Both the detection system and the automatic sample introduction system 101 are housed within the chamber 203. The temperature control device is mounted on the housing 202 and is used to regulate the temperature of the chamber 203. In this application, the "temperature of the chamber 203" refers to the temperature of the gas (generally air) filling the chamber 203. The temperature control device can communicate with a host computer, which can control the temperature of the chamber 203 by controlling the operation of the temperature control device, thereby ensuring the sample is at a suitable temperature to prevent sample deterioration and ensure the accuracy of the detection results.

[0055] Temperature control devices can have different settings. For example, refer to... Figure 6 In one embodiment, the temperature regulating device may include a constant-temperature heating platform 204. The constant-temperature heating platform 204 is readily available and inexpensive. The constant-temperature heating platform 204 includes an electric heating element (e.g., a heating tube) and a platform surface. The electric heating element is disposed below the platform surface. After the electric heating element is energized and heats up, the platform surface also heats up accordingly. At least a portion of the platform surface is located within the chamber 203, so that the heated platform surface can heat the air in the chamber 203, thereby bringing the chamber 203 to a suitable temperature. In another embodiment, for example, the temperature regulating device includes a temperature regulating plate located within the chamber 203. The temperature regulating plate has a flow channel within which a flowing heat exchange medium is disposed. By introducing heat exchange media of different temperatures (e.g., water) into the flow channel, the chamber 203 can reach different temperatures.

[0056] Reference Figure 6The SPR analyzer 201 may also include a temperature sensor 205, which is installed inside the housing 202 and used to detect the temperature of the chamber 203. The temperature sensor 205 can be directly connected to the temperature control device; alternatively, both the temperature sensor 205 and the temperature control device can be connected to a host computer, thus establishing communication between them. The operation of the temperature control device can be adjusted based on the detection results of the temperature sensor 205. For example, when the temperature of the chamber 203 is lower than the preset temperature, the temperature control device is activated and operates at a higher power (heating power); when the temperature of the chamber 203 is higher than the preset temperature, the heating power of the temperature control device is reduced or even reduced to zero. This configuration improves the automation level of the SPR analyzer 201, avoids frequent adjustments to the operating power of the temperature control device by the user, and also improves the accuracy of temperature control in the chamber 203.

[0057] It should be noted that certain components of the automatic sample introduction system 101 and the detection system can be connected to the housing 202. For example, the mounting base 108 of the second drive mechanism 106 and the first motor of the first drive mechanism 102 can be fixed to the housing 202 with screws, and the brackets used to fix components such as the flow cell 119, the diversion cell 117, and the prism 206 can also be fixed to the inner wall of the housing 202.

[0058] Reference Figure 5 The SPR analyzer 201 also includes a display screen 401, a fault light 402, a start button 403, an emergency stop button 44, and an observation window 405 mounted on the housing 202. The display screen 401, fault light 402, start button 403, and emergency stop button 44 are all connected to a host computer 406. The display screen 401 displays parameters such as the internal temperature of the housing 202, the angle of the movable plate 105, the position of the sampling needle 112, the pump power, and the sample flow rate, allowing the user to monitor whether the SPR analyzer 201 is operating normally. When the automatic sample introduction system 101 or the detection system malfunctions, the fault light 402 illuminates, alerting the user that the SPR analyzer 201 is not operating normally. The observation window 405 is located on the top of the housing and is made of glass or other transparent material. The user can observe the operating status of the automatic sample introduction system 101 and the detection system inside the housing through the observation window 405 to facilitate preliminary troubleshooting. When the SPR analyzer 201 needs to be used for sample analysis, the user can press the start button 403 to start the SPR analyzer 201; when it needs to be stopped due to malfunction or other reasons, the user can press the emergency stop button 44.

[0059] In the description of this utility model, the terms "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of this utility model. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

Claims

1. An automatic sample introduction system, characterized in that, include: A sample compartment includes a movable tray and multiple sample containers, wherein the sample containers are connected to the movable tray and are capable of holding samples; Sampling needle; A sampling tube includes an inlet end and an outlet end, wherein the inlet end is connected to the sampling needle; A first driving mechanism is connected to the movable disk. The first driving mechanism can drive the movable disk to move so that the sampling needle is positioned relative to different sample containers. A second driving mechanism is connected to the sampling needle. The second driving mechanism can drive the sampling needle to move up and down so that the bottom end of the sampling needle enters and exits the sample container. A pump is installed in the sampling tube. Driven by the pump, the sample in the sample container, which is positioned opposite the sampling needle, can enter the sampling tube through the sampling needle and flow to the outlet end of the sampling tube.

2. The automatic sample introduction system according to claim 1, characterized in that, The pump is a peristaltic pump.

3. The automatic sample introduction system according to claim 1, characterized in that, The movable disk is circular, and multiple sample containers surround the center of the movable disk. The first driving mechanism includes a first motor, which is connected to the center of the movable disk and can drive the movable disk to rotate.

4. The automatic sample introduction system according to claim 1, characterized in that, The second drive mechanism includes: Fixed base; The slider is fixedly connected to the sampling needle and slidably connected to the fixed base; The second motor is mounted on the fixed base; A lead screw is connected to the second motor and is vertically arranged. The lead screw is also threadedly connected to the slider. The second motor can drive the lead screw to rotate so that the slider rises and falls relative to the fixed seat.

5. The automatic sample introduction system according to claim 1, characterized in that, The automated sample delivery system also includes: A diversion tank is connected to the outlet end. The diversion tank is provided with multiple diversion channels, and the diversion channels are connected to the lumen of the sampling tube. A flow cell, the flow cell being transparent, the flow cell being provided with multiple detection channels, the number of the detection channels being the same as the number of the diversion channels; The number of connecting tubes is the same as the number of diversion channels. The two ends of the connecting tubes are respectively connected to the diversion pool and the flow pool. Each connecting tube connects one detection channel and one diversion channel.

6. The automatic sample introduction system according to claim 1, characterized in that, The automatic sampling system also includes a flow cell, which is transparent and connected to the outlet end. The flow cell is provided with a detection channel, and the lumen of the sampling tube is connected to the detection channel.

7. An SPR analyzer, characterized in that, Includes a detection system and an automated sample introduction system as described in claim 5 or 6, wherein the detection system includes: A prism, wherein the prism is transparent, and the side of the prism includes a light-incoming surface, a light-outcoming surface, and a detection surface that are connected end to end in sequence; An SPR sensor includes a chip body and a gold film. The chip body is transparent and connected to the detection surface. The gold film is connected to the side surface of the chip body facing away from the prism. The flow cell is connected to the side surface of the gold film facing away from the prism. A light source capable of emitting light, the light rays passing through the light-inlet surface into the prism and being reflected by the gold film before exiting from the light-outlet surface; An image acquisition device is disposed opposite to the light-emitting surface and is capable of acquiring the light emitted from the light-emitting surface.

8. The SPR analyzer according to claim 7, characterized in that, The SPR analyzer also includes: The housing has an internal chamber, in which both the detection system and the automatic sample introduction system are located; A temperature regulating device is installed in the housing and is used to regulate the temperature of the chamber.

9. The SPR analyzer according to claim 8, characterized in that, The temperature regulating device includes a constant temperature heating platform, which includes a heat-generating surface, at least a portion of which is located in the chamber.

10. The SPR analyzer according to claim 8, characterized in that, The SPR analyzer also includes a temperature sensor, which is installed in the housing and used to detect the temperature of the chamber. The temperature sensor is communicatively connected to the temperature regulating device, and the operating power of the temperature regulating device is adjusted according to the detection result of the temperature sensor.

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