Detection sample accommodating device

Abstract

The detection sample containing device is used for detecting blood parasites, blood is preprocessed to obtain a detection sample, the detection sample is added into a containing cavity of the detection sample containing device, and the detection sample containing device comprises a control module, a camera shooting assembly, a supporting assembly and an image AI calculation module; the control module is in electric signal connection with the camera component; the control module is in electric signal connection with the image AI calculation module; the supporting assembly is used for placing the detection sample accommodating device; the image AI calculation module comprises a storage unit, and the storage unit is used for storing blood parasite AI feature data; the camera assembly is used for shooting detection samples to obtain detection images; and the image AI calculation module is used for analyzing the detection image. A sample adding port of the detection sample accommodating device is communicated with the accommodating cavities A and B; the liquid containing height of the containing cavity A is higher than that of the containing cavity B; the accommodating cavity A is used for accommodating a detection sample of a species A; the accommodating cavity B is used for accommodating a detection sample of a species B.

Description

Technical Field

[0001] This application belongs to the field of formed element analysis technology based on magnified microscopic images, and specifically relates to a blood parasite detection device and a sample container. Background Technology

[0002] Bloodworms. According to Chai Jianyuan et al. (1990), six species of bloodworms have been found in turtles and tortoises in my country. Taking *Hydroceta sinensis* as an example, it exists in the Chinese soft-shelled turtle in three stages:

[0003] ① Mature schizonts within Kupffer cells of the liver are oval or spherical, measuring 17.92-18.24 μm × 14.84-15.24 μm, and produce 15-20 merozoites, which are short rod-shaped.

[0004] ② In deep blood, schizont proliferation occurs within the erythrocytes of the hepatic sinusoids. There are X-type and Y-type schizonts, both similar in size and shape, and both columnar. X-type schizonts produce 14-18 small, wedge-shaped merozoites; Y-type schizonts produce 4-8 large, rod-shaped merozoites. Female gametocytes are kidney-shaped, while male gametocytes are comma-shaped with a small tail at the posterior end.

[0005] ③ In peripheral blood cells, the early-stage schizont is broadly kidney-shaped and produces 6-12 merozoites, which are banana-shaped; the intermediate-stage schizont produces 6-10 merozoites; and the late-stage schizont produces 2 merozoites. Gametocytes originate from Y-type merozoites, and the vegetative body is the intermediate stage in the development from the previous generation of merozoites to the next generation. The Chinese soft-shelled turtle is the intermediate host of *Hydrocotyle sinensis*.

[0006] The leech *Hirudo medicinalis* is the definitive host of *Hydrocotyle sinensis*. After the leech feeds on the blood of a diseased turtle, *Hydrocotyle sinensis* develops within the leech's digestive tract, involving two stages: gametogony and spore reproduction. Gametogony is characterized by the fusion of male and female gametoblast mother cells before gamete differentiation, producing four male gamete nuclei. One of these nuclei fertilizes the female gamete nucleus, forming a zygote. Spore reproduction begins with nuclear division of a mononuclear oocyst, eventually forming a mature oocyst containing eight gymnosporidis, which then disintegrates to release sporospores.

[0007] The applicant has filed a series of Chinese patents, such as

[0008] 1. CN2020112669290, "Cellular analysis methods and systems and quantitative methods and systems";

[0009] 2. CN2020112669182, "Imaging methods, systems and kits for cell suspension samples";

[0010] 3. CN2022104799126, "A method for rapid focusing of a microscopic image acquisition device and a method for acquiring microscopic images";

[0011] 4. CN2023110496905, "A device, chip and method for detecting formed elements in blood, urine and feces (multi-parameter)".

[0012] A brand-new technical solution is used to measure the content of target substances in blood.

[0013] Blood parasite testing, combined with an understanding of the host, characteristic morphological structures found in the blood, and various forms of parasites, helps in the diagnosis of specific parasite species and precise medication. It can reveal the parasite infection status of multiple systems in the body, and early detection and treatment can help in health management and monitoring and the prevention of zoonotic diseases.

[0014] There are many types of parasites in the blood, and different parasitic infections require different clinical interventions. Accurate identification of the different parasite types is crucial for providing appropriate interventions. Furthermore, the state of the parasites can vary significantly at different stages of infection, necessitating precise interventions tailored to each stage.

[0015] However, in large-scale practical applications, collecting samples of different types of parasite infections is time-consuming, labor-intensive, and inefficient; collecting samples of parasites at different stages of infection is even more difficult. This makes large-scale AI training challenging, hindering accurate identification of parasite species or precise identification of the infection stage.

[0016] There is currently no specialized equipment for treating blood parasites. Summary of the Invention

[0017] In this application, the inventors propose a device and a sample container specifically for detecting blood parasites; the detection of blood parasites is performed using an image AI computing module.

[0018] The technical solution of this application to solve the above-mentioned technical problems is a blood parasite detection device, used to detect blood parasites. The blood is pre-treated to obtain a test sample, which is then added to the receiving cavity of a test sample receiving device. The device includes a control module, a camera assembly, a support assembly, and an image AI computing module. The control module is electrically connected to the camera assembly and the image AI computing module. The support assembly is used to place the test sample receiving device. The image AI computing module includes a storage unit for storing blood parasite AI feature data. The camera assembly is used to capture images of the test sample to obtain test images. The image AI computing module is used to analyze the test images.

[0019] The blood parasite detection device includes any one or more of the following technical features: TA10: the image AI computing module is located within the control module; TA20: the control module includes a network component; the image AI computing module is located in a server, and the network component is electrically connected to the image AI computing module via a network.

[0020] The camera assembly includes a Z-axis slide assembly and a microscope camera assembly. The microscope camera assembly is mechanically connected to the slide in the Z-axis slide assembly, and the microscope camera assembly can move in the Z-axis direction.

[0021] The blood parasite detection device further includes a drive module, a control module electrically connected to the drive module, and a drive module electrically connected to the support component. The support component includes an X-slide assembly and a sample receiving device placed on the slide of the X-slide assembly. The drive module is used to drive the X-slide assembly to move in the X-axis direction, thereby moving the sample receiving device in the X-axis direction.

[0022] The blood parasite detection device includes a support component comprising a Y-slide assembly and an X-slide assembly placed on the slide of the Y-slide assembly. A drive module is used to drive the Y-slide assembly to move in the Y-axis direction, thereby moving the sample container in the X-axis or Y-axis direction.

[0023] The blood parasite detection device includes any one of the following technical features: TB10: further includes circuit board A and circuit board B, the control module is disposed on circuit board A, and the drive module is disposed on circuit board B; TB20: further includes circuit board C, the drive module and the control module are disposed on circuit board C.

[0024] The blood parasite detection device includes any one or more of the following technical features: TC10: the support assembly includes a light source assembly, the light source assembly includes a white light source, and the white light emitted by the white light source is used to illuminate the receiving cavity; TC20: the support assembly includes a light source assembly, the light source assembly includes a purple light source, and the purple light emitted by the purple light source is used to illuminate the receiving cavity; TC30: the camera assembly is above or below the support assembly.

[0025] The technical solution of this application to solve the above-mentioned technical problems can also be a sample containing device for blood parasite detection. The blood is pretreated to obtain a test sample, which is added to the containing cavity of the sample containing device, including containing cavity A, containing cavity B, and a sample inlet. The sample inlet is used to add the test sample to be tested. The sample inlet is connected to containing cavity A. The sample inlet is connected to containing cavity B. The liquid containing height of containing cavity A is higher than the liquid containing height of containing cavity B. Containing cavity A is used to contain the test sample of species A. Containing cavity B is used to contain the test sample of species B.

[0026] The sample containment device includes any one or more of the following technical features: D10: The containment chambers A and B are connected in series for sequentially filling the containment chambers A and B with the liquid to be tested; TD20: The containment chambers A and B are connected in parallel for simultaneously filling the containment chambers A and B with the liquid to be tested; TD30: The containment chambers A and B are connected in parallel but not connected for separately filling the containment chambers A and B with the liquid to be tested; TD40: It also includes an exhaust port; the exhaust port is connected to containment chamber A; the exhaust port is connected to containment chamber B; and the exhaust port is connected to the external atmosphere.

[0027] The aforementioned sample containing device includes any one of the following technical features: Feature TE10: the upper part of the containing cavity A and the upper part of the containing cavity B are made of transparent material; Feature TE20: the lower part of the containing cavity A and the lower part of the containing cavity B are made of transparent material; Feature TE30: the containing cavity A and the containing cavity B have the same bottom height; Feature TE40: the containing cavity A and the containing cavity B have the same top height.

[0028] The technical effects of the above-mentioned technical solution include: the setting of control module, camera component, support component, and image AI computing module provides a dedicated detection device for occasional blood parasitic diseases. With the help of AI, cells infected by blood parasites can be identified from a massive number of cells, greatly improving the identification efficiency.

[0029] The technical advantages of the above-mentioned technical solution include: the image AI computing module is located within the control module, making the device more efficient and compact.

[0030] The technical effects of the above technical solution include: the control module includes a network component; the image AI computing module is arranged in the server, and the network component is electrically connected to the image AI computing module through the network, which facilitates real-time updates of the image AI computing module and is conducive to evolution.

[0031] The technical effects of the above solution include: the camera assembly includes a Z-axis slide assembly and a microscope camera assembly; the microscope camera assembly is mechanically connected to the slide in the Z-axis slide assembly, allowing the microscope camera assembly to move in the Z-axis direction. This facilitates image acquisition, provides a device for precisely adjusting the imaging-related distance, and makes it easier to obtain clear images.

[0032] The technical effects of the above solution include: the drive module drives the X-slide assembly to move in the X-axis direction, thereby moving the sample receiving device in the X-axis direction. The drive module also drives the Y-slide assembly to move in the Y-axis direction, thereby moving the sample receiving device in either the X-axis or Y-axis direction. Moving the sample receiving device in either the X-axis or Y-axis direction facilitates the acquisition of images from different positions, improving image acquisition and analysis efficiency.

[0033] The technical advantages of the above solution include: the control module is set on circuit board A, and the drive module is set on circuit board B. The separate setup helps to reduce maintenance costs.

[0034] The technical advantages of the above solution include: the drive module and control module are located on circuit board C, making the device more compact and reducing overall cost.

[0035] The technical effects of the above-mentioned technical solution include: the light source component includes a white light source, the white light emitted by the white light source is used to illuminate the containment cavity, and the strong white light can provide multiple spectra, which is beneficial to the identification of bloodworms.

[0036] The technical effects of the above-mentioned technical solution include: the purple light emitted by the purple light source is used to illuminate the containment cavity; the purple light source is particularly beneficial for the identification of bloodworms in cells, especially for some animals whose red blood cells are nucleated red blood cells, the ultraviolet light source is very helpful in distinguishing the cell nucleus of red blood cells from bloodworms.

[0037] The technical effects of the above-mentioned technical solution include: the camera component is located above or below the support component, compatible with both vertical imaging methods, and the way images are obtained is more flexible.

[0038] The technical effects of the above-mentioned technical solution include: the sample container device is equipped with two container chambers, A and B, which can further improve the detection efficiency of blood parasitic diseases.

[0039] The technical effects of the above-mentioned technical solution include: different connection and communication methods for cavity A and cavity B, adapting to a variety of different application scenarios and improving efficiency.

[0040] The technical effects of the above-mentioned technical solution include: the upper part of the receiving cavity A and the upper part of the receiving cavity B are made of transparent material, and the lower part of the receiving cavity A and the lower part of the receiving cavity B are made of transparent material, which facilitates the application of the method of imaging from top to bottom.

[0041] The technical effects of the above-mentioned technical solution include: the accommodating cavity A and the accommodating cavity B have the same bottom or top height, so that even if the cavity height of the accommodating cavity A is different from that of the accommodating cavity B, it is convenient to perform imaging with the bottom or top as a reference, and they have a common imaging reference plane. Attached Figure Description

[0042] Figures 1 to 6 This is a schematic diagram of a blood parasite detection device. Figures 1 to 6 ;

[0043] Figures 7 to 10 This is a schematic diagram of a sample container. Figures 1 to 5 . Detailed Implementation

[0044] The contents of this application will be further described in detail below with reference to the accompanying drawings. It should be noted that the following description is of preferred embodiments of the present invention and does not constitute any limitation on the present invention. The description of the preferred embodiments of the present invention is merely an explanation of the general principles of the invention. The designations "first," "second," "A," and "B" used in this invention are for ease of explanation only and do not represent a temporal or spatial order. The combinations of letters and numbers "TA," "TB," and "H" used in this invention are for ease of explanation only, and their specific meanings are determined by the specific terms they represent.

[0045] like Figure 1 A blood parasite detection device is disclosed for detecting blood parasites. The blood is pre-treated to obtain a test sample, which is then added to the receiving cavity of a test sample receiving device. The device includes a control module, a camera assembly, a support assembly, and an image AI computing module. The control module is electrically connected to the camera assembly and the image AI computing module. The support assembly is used to hold the test sample receiving device. The image AI computing module includes a storage unit for storing AI feature data of the blood parasites. The camera assembly is used to capture images of the test sample to obtain test images. The image AI computing module is used to analyze the test images.

[0046] like Figure 1 The image AI computing module is located within the control module.

[0047] like Figure 3 The control module includes a network component; the image AI computing module is located in the server, and the network component is electrically connected to the image AI computing module via a network.

[0048] like Figure 4 The camera assembly includes a Z-axis slide assembly and a microscope camera assembly. The microscope camera assembly is mechanically connected to the slide in the Z-axis slide assembly, and the microscope camera assembly can move in the Z-axis direction. The drive module is electrically connected to the camera assembly and the Z-axis slide assembly, and the drive module is used to drive the microscope camera assembly to move in the Z-axis direction.

[0049] like Figures 2 to 4 It also includes a drive module, a control module that is electrically connected to the drive module, and a drive module that is electrically connected to the support component. The support component includes an X-slide assembly and a sample receiving device that is placed on the slide of the X-slide assembly. The drive module is used to drive the X-slide assembly to move in the X-axis direction, thereby moving the sample receiving device in the X-axis direction.

[0050] like Figure 4The support components include a Y-slide assembly and an X-slide assembly placed on the slide of the Y-slide assembly. The drive module is used to drive the Y-slide assembly to move in the Y-axis direction, thereby moving the sample receiving device in the X-axis or Y-axis direction.

[0051] like Figure 5 The blood parasite detection device also includes circuit board A and circuit board B, with the control module mounted on circuit board A and the drive module mounted on circuit board B.

[0052] like Figure 6 The blood parasite detection device also includes a circuit board C, and the drive module and control module are mounted on the circuit board C.

[0053] like Figures 1 to 6 The support components include a light source assembly, which includes a white light source. The white light emitted by the white light source is used to illuminate the receiving cavity.

[0054] like Figures 1 to 6 The supporting components include a light source assembly, which includes a purple light source. The purple light emitted by the purple light source is used to illuminate the receiving cavity.

[0055] like Figures 1 to 6 The camera assembly is positioned above the support assembly. In other embodiments, the camera assembly is positioned below the support assembly.

[0056] like Figures 7 to 10 A sample container is used for blood parasite detection. The blood is pretreated to obtain a test sample, which is then added to the container chambers of the sample container, including container chamber A, container chamber B, and a sample inlet. The sample inlet is used to add the test sample. The sample inlet is connected to container chamber A and container chamber B. The liquid level of container chamber A is higher than that of container chamber B. Container chamber A is used to hold the test sample of species A, and container chamber B is used to hold the test sample of species B.

[0057] like Figure 7 The receiving cavities A and B are connected in series and are used to sequentially pour the liquid to be tested into receiving cavities A and B.

[0058] like Figure 8 The receiving cavities A and B are connected in parallel and are used to simultaneously fill the receiving cavities A and B with the liquid to be tested.

[0059] like Figure 9 and Figure 10 The receiving cavities A and B are connected in parallel but not interconnected, and are used to pour the liquid to be tested into receiving cavities A and B respectively.

[0060] like Figures 7 to 10 The sample container also includes an exhaust port; the exhaust port is connected to the container cavity A; the exhaust port is connected to the container cavity B; and the exhaust port is connected to the outside atmosphere.

[0061] like Figures 7 to 10 The upper part of cavity A and the upper part of cavity B are made of transparent material.

[0062] In some embodiments, the lower part of the receiving cavity A and the lower part of the receiving cavity B are made of transparent material.

[0063] In some embodiments, the receiving cavity A and the receiving cavity B have the same bottom height.

[0064] In some embodiments, the receiving cavity A and the receiving cavity B have the same height at the top.

[0065] While the present invention has been described and illustrated with reference to preferred embodiments and several alternatives, the invention is not limited to the specific descriptions herein. Other alternatives or equivalent components may also be used to practice the invention.

Claims

1. A sample receiving device for detecting blood parasites, wherein the blood is pretreated to obtain a sample for testing, and the sample is added to the receiving cavity of the sample receiving device, characterized in that: It includes a receiving cavity A, a receiving cavity B, and a sample inlet; the sample inlet is used to add the sample to be tested; the sample inlet is connected to the receiving cavity A; the sample inlet is connected to the receiving cavity B; the liquid holding height of the receiving cavity A is higher than the liquid holding height of the receiving cavity B; Chamber A is used to hold the test sample of species A; Chamber B is used to hold the test sample of species B.

2. The sample container according to claim 1, characterized in that, Includes one or more of the following technical features: TD10: The receiving cavity A and receiving cavity B are connected in series and are used to sequentially pour the liquid to be tested into receiving cavity A and receiving cavity B; TD20: The receiving cavity A and receiving cavity B are connected in parallel for the simultaneous filling of the liquid to be tested into receiving cavity A and receiving cavity B; TD30: The receiving cavity A and receiving cavity B are connected in parallel but not interconnected, and are used to pour the liquid to be tested into receiving cavity A and receiving cavity B respectively; TD40: also includes an exhaust port; the exhaust port is connected to the receiving cavity A; the exhaust port is connected to the receiving cavity B; the exhaust port is connected to the external atmosphere.

3. The sample container according to claim 1, characterized in that, Includes any one of the following technical features: Feature TE10: The upper part of the receiving cavity A and the upper part of the receiving cavity B are made of transparent material; Feature TE20: The lower part of the receiving cavity A and the lower part of the receiving cavity B are made of transparent material; Feature TE30: The receiving cavity A and the receiving cavity B have the same bottom height; Feature TE40: The receiving cavity A and the receiving cavity B have the same height at the top.

Images