Fish Igm monoclonal antibody affinity purification column

Abstract

The utility model discloses a fish Igm monoclonal antibody affinity purification column which comprises a base, and the right side of the base is fixedly connected with a telescopic structure; the telescopic structure comprises a power assembly and a connecting assembly; the power assembly comprises a telescopic bottom column. Due to the arrangement of the telescopic structure, when the purification column needs to adjust the distance between the purification discs according to different antibodies, an operator can pull the telescopic middle column, and then the fixed column II on the inner side of the telescopic bottom column drives the transmission belt to anticlockwise rotate towards the left side; by changing the distance between a telescopic middle column and a telescopic bottom column, the telescopic upper column is driven to adjust the position, and then the purification discs on the inner sides of the telescopic middle column and the telescopic upper column change the distance; different antibodies can be conveniently purified, so that the antibodies are more completely purified.

Description

TECHNICAL FIELD

[0001] The utility model relates to antibody purification technical field, concretely is a fish Igm monoclonal antibody affinity purification column. BACKGROUND

[0002] Monoclonal antibody is the antibody of highly uniform, only aiming at certain specific epitope produced by single B cell cloning. Usually adopt hybridoma technology to prepare, hybridoma antibody technology is on the basis of cell fusion technology, and the sensitized B cell with the ability of secreting specific antibody and the myeloma cell with the unlimited propagation ability are fused into B cell hybridoma, and the single hybridoma cell with this characteristic is cultured into cell group, and the specific antibody for one antigen epitope, i. e. monoclonal antibody, can be prepared. The fish Igm monoclonal antibody affinity purification column of the prior art is difficult to adjust the purification according to different monoclonal antibodies when the fish, for example, mouse anti-tilapia Igm monoclonal antibody is subjected to antibody purification, and the single antibody purification can easily lead to unstable antibody purification results, which has limitations. In view of the above problems, a fish Igm monoclonal antibody affinity purification column is provided.

[0003] Application No. 202321127377.4 discloses an affinity protein purification chromatography column, which comprises a column body, a filler cylinder and an extrusion assembly. The top of the column body is provided with a cover, and the cover is provided with a liquid inlet pipe. The bottom of the column body is connected with a liquid outlet pipe. The filler cylinder is arranged in the column body, and a liquid passing gap is arranged between the filler cylinder and the column body. The filler cylinder is provided with a first liquid outlet hole. The extrusion assembly is threadedly connected to the cover. The liquid inlet pipe extends into the filler and is provided with a first liquid inlet hole on the outer peripheral wall. The affinity protein purification chromatography column provided by the utility model has the advantages that the solution enters the filler cylinder filled with the filler through the liquid inlet pipe, the chromatography liquid is discharged from the first liquid outlet hole of the filler cylinder into the liquid passing gap, and then is discharged through the liquid outlet pipe. The liquid inlet pipe extends into the filler, so that the solution is uniformly dispersed in the filler. Under the extrusion of the extrusion assembly, the chromatography liquid is separated from the filler and discharged into the liquid passing gap, thereby ensuring the purification quality of the solution and improving the utilization rate of the filler.

[0004] However, the solution of the chromatography column enters the filler cylinder filled with the filler through the liquid inlet pipe, the chromatography liquid is discharged from the first liquid outlet hole of the filler cylinder into the liquid passing gap, and then is discharged through the liquid outlet pipe. The liquid inlet pipe extends into the filler, so that the solution is uniformly dispersed in the filler. Under the extrusion of the extrusion assembly, the chromatography liquid is separated from the filler and discharged into the liquid passing gap, thereby ensuring the purification quality of the solution and improving the utilization rate of the filler. However, when the fish Igm monoclonal antibody is subjected to antibody purification, it is difficult to adjust the purification according to different monoclonal antibodies, and the single antibody purification can easily lead to unstable antibody purification results, which has limitations. UTILITY MODEL CONTENT

[0005] The utility model discloses a fish Igm monoclonal antibody affinity purification column, to solve the solution of the chromatographic column in the background art is entered the filler cylinder that is equipped with filler through the liquid inlet pipe, and the chromatographic solution is discharged from the first liquid outlet hole on the filler cylinder to the liquid gap, and is discharged through the liquid outlet pipe, and the liquid inlet pipe extends to the inside of filler, makes the chromatographic solution from the filler and is discharged to the liquid gap under the extrusion of the extrusion component, guarantees the purification quality of solution, improves the utilization of filler, but the purification column is difficult to adjust the purification according to different monoclonal antibodies when carrying out the antibody purification of fish Igm monoclonal antibody, and the single antibody purification is easy to cause the result of antibody purification to be not stable, has the problem of limited.

[0006] To solve the above technical problem, the utility model is realized through the following technical schemes:

[0007] The utility model discloses a fish Igm monoclonal antibody affinity purification column, including base:

[0008] The right side fixed connection of base has telescopic structure;

[0009] The telescopic structure includes power component and connecting component;

[0010] The power component includes telescopic bottom column, fixed column no.

[0011] The right side fixed connection of base has telescopic bottom column, the inboard fixed connection of telescopic bottom column has fixed column no.

[0012] Further, two symmetrical rotors are provided, and the two symmetrical rotors are connected by a transmission belt, and the connecting column is fixedly connected to the inside of the telescopic bottom column and the fixed column no.

[0013] Further, the power component further includes extension column no.

[0014] The outside fixed connection of rotator no.

[0015] Further, the inside of purification disc no.

[0016] Further, the connecting assembly comprises the fixed column three, the connecting column two, the connecting column three, the connecting shaft, the connecting column four and the bolt column;

[0017] The outer side of the telescopic middle column is fixedly connected with the fixed column three, the inner side of the fixed column three is movably connected with the connecting column two, the outer side of the connecting column two is movably connected with the connecting column three, the inner side of the connecting column three is movably connected with the connecting shaft, the outer side of the connecting shaft is movably connected with the connecting column four, and the lower side of the connecting column four is fixedly connected with the bolt column.

[0018] Further, the fixed column three is connected through the connecting column two and the connecting column three, the connecting column two is connected through the connecting column three and the connecting column four, and the connecting column four is connected through the connecting shaft and the bolt column.

[0019] Further, the purification structure comprises the connecting column five, the extension column two, the telescopic upper column, the bolt slot, the purification disc two and the feeding port.

[0020] The outer side of the transmission belt is fixedly connected with the connecting column five, the right side of the connecting column five is fixedly connected with the extension column two, the side, away from the connecting column five, of the extension column two is fixedly connected with the telescopic upper column, the inner side of the telescopic upper column is provided with the bolt slot, the inner side of the telescopic upper column is fixedly connected with the purification disc two, and the right side of the telescopic upper column is fixedly connected with the feeding port.

[0021] Further, the bolt slot is symmetrically provided with three, the bolt slot penetrates through the telescopic middle column and the telescopic upper column, and the bolt slot is connected with the bolt column through the telescopic upper column and the telescopic middle column.

[0022] The inner side of the purification disc two is provided with a plurality of O-shaped grooves, and the inner diameter of the O-shaped groove in the inner side of the purification disc two is slightly larger than the inner diameter of the O-shaped groove in the inner side of the purification disc one.

[0023] The utility model has the following beneficial effects:

[0024] One, the utility model discloses a telescopic structure, when the purification column needs to adjust the distance between the purification disc according to different antibodies, the operator can pull the telescopic middle column, and then the fixed column two in the inner side of telescopic bottom column drives the transmission belt to rotate counterclockwise to the left side, and then the connecting column five on the outer side of the transmission belt drives the telescopic upper column to rotate counterclockwise to the right side, realizes the function of telescopic, drives the telescopic upper column to adjust the position by changing the distance between the telescopic middle column and the telescopic bottom column, and then the purification disc in the inner side of the telescopic middle column and the telescopic upper column changes the distance, and through the setting of a plurality of O-shaped grooves, different antibodies are purified conveniently, and the antibody purification is more complete.

[0025] Two, based on the above beneficial effects, also includes a purification structure, by re-purification disc two and purification disc one inside are provided with several O-shaped groove, but the O-shaped groove inside diameter size of purification disc two is slightly larger than the O-shaped groove inside diameter size of purification disc one, when the antibody enters through the inlet, first by the inner diameter of larger purification disc two and then through the inner diameter of smaller purification disc one, through the structure of layered purification, so that the purification is more complete. BRIEF DESCRIPTION OF DRAWINGS

[0026] In order to more clearly illustrate the technical scheme of the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiment description, obviously, the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained according to these drawings without creative labor.

[0027] Figure 1 It is a schematic diagram of the overall structure of the present application;

[0028] Figure 2 It is a schematic diagram of the extension structure of the present application;

[0029] Figure 3 It is a schematic diagram of the extension structure of the present application;

[0030] Figure 4 It is a schematic diagram of the extension structure of the present application; Figure 2 It is an enlarged schematic diagram of A in the present application;

[0031] Figure 5 It is a schematic diagram of the extension structure of the present application;

[0032] In the drawings, the components represented by each reference numeral are listed as follows:

[0033] In the drawings: 1, base; 2, extension structure; 3, purification structure; 201, extension bottom column; 202, fixed column one; 203, rotating disc one; 204, transmission belt; 205, connecting column one; 206, fixed column two; 207, extension column one; 208, extension middle column; 209, purification disc one; 210, fixed column three; 211, connecting column two; 212, connecting column three; 213, connecting shaft; 214, connecting column four; 215, bolt column; 301, connecting column five; 302, extension column two; 303, extension upper column; 304, bolt slot; 305, purification disc two; 306, inlet. DETAILED DESCRIPTION

[0034] With reference to the drawings of the embodiments of the present application, the technical solutions in the embodiments of the present application will be clearly and completely described, obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments of the present application, all other embodiments obtained by the person skilled in the art without creative labor are within the protection scope of the present application.

[0035] In order to make the purpose, technical scheme and advantages of the present application more clear, the embodiments of the present application will be further described in detail below with reference to the drawings.

[0036] Please refer to Figures 1-5 The utility model discloses a fish IgM monoclonal antibody affinity purification column, including base 1:

[0037] The right side of base 1 is fixedly connected with telescopic structure 2;

[0038] Telescopic structure 2 includes power assembly and connecting assembly;

[0039] Power assembly includes telescopic bottom column 201, fixed column one 202, rotating disc one 203, transmission belt 204, connecting column one 205 and fixed column two 206;

[0040] The right side of base 1 is fixedly connected with telescopic bottom column 201, the inner side of telescopic bottom column 201 is fixedly connected with fixed column one 202, the outer side of fixed column one 202 is movably connected with rotating disc one 203, the outer side of rotating disc one 203 is movably connected with transmission belt 204, the outer side of transmission belt 204 is fixedly connected with connecting column one 205, and the upper end of connecting column one 205 is fixedly connected with fixed column two 206.

[0041] Two symmetrical rotating disc one 203 are arranged, and the two symmetrical rotating disc one 203 are connected through transmission belt 204, and connecting column one 205 is fixedly connected through the inner side of fixed column two 206 and telescopic bottom column 201.

[0042] When the purification column needs to adjust the distance between the purification discs according to different antibodies, the operator can pull telescopic middle column 208, change the distance between telescopic middle column 208 and telescopic bottom column 201, drive telescopic upper column 303 to adjust the position, and then the purification discs in the inner side of telescopic middle column 208 and telescopic upper column 303 change the distance, which is convenient for purifying different antibodies and makes the antibody purification more complete.

[0043] Power assembly further includes extension column one 207, telescopic middle column 208 and purification disc one 209;

[0044] The outer side of the rotating disc 203 is fixedly connected with an extension column 207, the right side of the extension column 207 is fixedly connected with a telescopic middle column 208, and the inner side of the telescopic middle column 208 is fixedly connected with a purification disc 209.

[0045] The inner side of the purification disc 209 is provided with a plurality of O-shaped grooves which penetrate the inner side of the purification disc 209.

[0046] The plurality of O-shaped grooves make the antibody purification more complete and not limited to the process of purifying a single antibody.

[0047] The connecting assembly comprises a fixed column 210, a connecting column 211, a connecting column 212, a connecting shaft 213, a connecting column 214 and a bolt column 215.

[0048] The outer side of the telescopic middle column 208 is fixedly connected with the fixed column 210, the inner side of the fixed column 210 is movably connected with the connecting column 211, the outer side of the connecting column 211 is movably connected with the connecting column 212, the inner side of the connecting column 212 is movably connected with the connecting shaft 213, the outer side of the connecting shaft 213 is movably connected with the connecting column 214, and the lower side of the connecting column 214 is fixedly connected with the bolt column 215.

[0049] The fixed column 210 is connected through the connecting column 211 and the connecting column 212, the connecting column 211 is connected through the connecting column 212 and the connecting column 214, and the connecting column 214 is connected through the connecting shaft 213 and the bolt column 215.

[0050] When the distance between the telescopic middle column 208 and the telescopic bottom column 201 is expanded to a certain extent, the operator can rotate the angle of the connecting column assembly, thereby driving the bolt column 215 to be inserted into the bolt groove 304, and then fixing the bolt column 215 in the groove, thereby fixing the telescopic position of the telescopic middle column 208 and the telescopic upper column 303.

[0051] Working principle: when the purification column needs to adjust the distance between the purification discs according to different antibodies, the operator can pull the telescopic middle column 208, thereby driving the fixed column 206 inside the telescopic bottom column 201 to rotate the transmission belt 204 to the left side along the counterclockwise direction, thereby driving the connecting column 301 outside the transmission belt 204 to rotate the telescopic upper column 303 to the right side along the counterclockwise direction, realizing the telescopic function, changing the distance between the telescopic middle column 208 and the telescopic bottom column 201 to drive the telescopic upper column 303 to adjust the position, and then changing the distance between the purification discs inside the telescopic middle column 208 and the telescopic upper column 303, and through the plurality of O-shaped grooves, the different antibodies are purified, and the antibody purification is more complete.

[0052] This step makes the distance between the purification discs adjustable, and the purification of the antibody is more complete.

[0053] Referring to Figures 1-5 As shown in the drawings, the embodiment is based on the above embodiment one, further comprising a purification structure 3: the purification structure 3 comprises a connecting column five 301, an extension column two 302, a telescopic upper column 303, a bolt slot 304, a purification disc two 305 and a feeding port 306;

[0054] The outer side of the transmission belt 204 is fixedly connected with the connecting column five 301, the right side of the connecting column five 301 is fixedly connected with the extension column two 302, the side, away from the connecting column five 301, of the extension column two 302 is fixedly connected with the telescopic upper column 303, the inner side of the telescopic upper column 303 is provided with the bolt slot 304, the inner side of the telescopic upper column 303 is fixedly connected with the purification disc two 305, and the right side of the telescopic upper column 303 is fixedly connected with the feeding port 306.

[0055] The bolt slot 304 is symmetrically provided with three, the bolt slot 304 penetrates through the telescopic middle column 208 and the telescopic upper column 303, and the bolt slot 304 is connected with the bolt column 215 through the telescopic upper column 303 and the telescopic middle column 208.

[0056] The inner side of the purification disc two 305 is provided with a plurality of O-shaped grooves, and the inner diameter of the O-shaped groove in the inner side of the purification disc two 305 is slightly larger than that of the O-shaped groove in the inner side of the purification disc one 209.

[0057] The inner sides of the purification disc two 305 and the purification disc one 209 are both provided with a plurality of O-shaped grooves, but the inner diameter of the O-shaped groove in the inner side of the purification disc two 305 is slightly larger than that of the O-shaped groove in the inner side of the purification disc one 209, when the antibody enters through the feeding port, it first passes through the purification disc two 305 with a larger inner diameter and then passes through the purification disc one 209 with a smaller inner diameter, so that the purification is more complete.

[0058] Working principle: the inner sides of the purification disc two 305 and the purification disc one 209 are both provided with a plurality of O-shaped grooves, but the inner diameter of the O-shaped groove in the inner side of the purification disc two 305 is slightly larger than that of the O-shaped groove in the inner side of the purification disc one 209, when the antibody enters through the feeding port, it first passes through the purification disc two 305 with a larger inner diameter and then passes through the purification disc one 209 with a smaller inner diameter, so that the purification is more complete.

[0059] In the description of the present application, it should be further explained that, unless otherwise specified and limited, the terms "setting", "installing", "connecting", "connecting" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the communication inside two elements. For ordinary skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0060] The preferred embodiments disclosed above are only used to help describe the utility model. The preferred embodiments do not describe all the details and do not limit the utility model to the specific embodiments. Obviously, according to the content of the specification, many modifications and changes can be made. The specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the utility model, so that the persons skilled in the art can well understand and utilize the utility model. The utility model is limited by the claims and the entire scope and equivalents thereof.

Claims

1. A fish IgM monoclonal antibody affinity purification column, characterized in that, Including the base (1): The right side of the base (1) is fixedly connected with the telescopic structure (2); The telescopic structure (2) comprises a power assembly and a connecting assembly; The power assembly comprises a telescopic bottom column (201), a fixed column (202), a rotating disc (203), a transmission belt (204), a connecting column (205) and a fixed column (206); The right side of the base (1) is fixedly connected with the telescopic bottom column (201), the inner side of the telescopic bottom column (201) is fixedly connected with the fixed column (202), the outer side of the fixed column (202) is movably connected with the rotating disc (203), the outer side of the rotating disc (203) is movably connected with the transmission belt (204), the outer side of the transmission belt (204) is fixedly connected with the connecting column (205), and the upper end of the connecting column (205) is fixedly connected with the fixed column (206).

2. The fish IgM monoclonal antibody affinity purification column according to claim 1, characterized in that: The rotating disc (203) is symmetrically provided with two, and the two symmetrically provided rotating discs (203) are connected through the transmission belt (204), and the connecting column (205) is fixedly connected through the inner side of the fixed column (206) and the telescopic bottom column (201).

3. The fish IgM monoclonal antibody affinity purification column according to claim 1, characterized in that: The power assembly further comprises an extension column (207), a telescopic middle column (208) and a purification disc (209); The outer side of the rotating disc (203) is fixedly connected with the extension column (207), the right side of the extension column (207) is fixedly connected with the telescopic middle column (208), and the inner side of the telescopic middle column (208) is fixedly connected with the purification disc (209).

4. The fish IgM monoclonal antibody affinity purification column according to claim 3, characterized in that: The inner side of the purification disc (209) is provided with a plurality of O-shaped grooves, and the O-shaped grooves penetrate the inner side of the purification disc (209).

5. The fish IgM monoclonal antibody affinity purification column according to claim 3, characterized in that: The connecting assembly comprises a fixed column (210), a connecting column (211), a connecting column (212), a connecting shaft (213), a connecting column (214) and a bolt column (215); The outer side of the telescopic middle column (208) is fixedly connected with the fixed column (210), the inner side of the fixed column (210) is movably connected with the connecting column (211), the outer side of the connecting column (211) is movably connected with the connecting column (212), the inner side of the connecting column (212) is movably connected with the connecting shaft (213), the outer side of the connecting shaft (213) is movably connected with the connecting column (214), and the lower side of the connecting column (214) is fixedly connected with the bolt column (215).

6. The fish IgM monoclonal antibody affinity purification column according to claim 5, characterized in that: The fixed column (210) is connected through the connecting column (211) and the connecting column (212), the connecting column (211) is connected through the connecting column (212) and the connecting column (214), and the connecting column (214) is connected through the connecting shaft (213) and the bolt column (215).

7. The fish IgM monoclonal antibody affinity purification column according to claim 1, characterized in that, It also includes a purification structure (3): the purification structure (3) comprises a connecting column (301), an extension column (302), a telescopic upper column (303), a bolt groove (304), a purification disc (305) and a feed inlet (306); The outer side of the transmission belt (204) is fixedly connected with a connecting column five (301), the right side of the connecting column five (301) is fixedly connected with an extension column two (302), the side away from the connecting column five (301) of the extension column two (302) is fixedly connected with a telescopic upper column (303), the inner side of the telescopic upper column (303) is provided with a bolt slot (304), the inner side of the telescopic upper column (303) is fixedly connected with a purification disc two (305), the right side of the telescopic upper column (303) is fixedly connected with a feed inlet (306).

8. The fish IgM monoclonal antibody affinity purification column according to claim 7, characterized in that: The bolt slot (304) is symmetrically provided with three, the bolt slot (304) penetrates the telescopic middle column (208) and the telescopic upper column (303), the bolt slot (304) is connected with the bolt column (215) through the telescopic upper column (303) and the telescopic middle column (208). The inner side of the purification disc two (305) is provided with a plurality of O-shaped grooves, the inner diameter size of the O-shaped groove on the inner side of the purification disc two (305) is slightly larger than that of the O-shaped groove on the inner side of the purification disc one (209).

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