Strain culture dish for preparing itaconic acid

Abstract

The utility model discloses a strain culture dish for preparing itaconic acid, relates to the field of culture dishes, and aims to solve the problems that culture of anaerobic bacteria is affected and bacteria in air are easy to pollute the culture dish due to the fact that a dish cover needs to be opened when a traditional culture dish is used for sampling in the prior art. A dish cover is connected to the upper end of the culture dish, an insertion hole is formed in one side of the middle of the upper end of the dish cover, a fixing pipe is fixedly connected to the upper end face of the dish cover and at the upper end of the insertion hole, a sampling cup is fixedly arranged in the fixing pipe, a sampling cavity is formed in the sampling cup, and a soft suction pipe is fixedly connected to the middle of the lower end face of the sampling cup. A rubber plug is fixedly arranged between the upper end of the sampling cavity of the sampling cup and the upper end surface of the sampling cup.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of culture dish, concretely to a strain culture dish for preparing itaconic acid. BACKGROUND

[0002] Itaconic acid is named as methylidene succinic acid, methylene butanedioic acid, is an unsaturated binary organic acid, chemical formula is C5H6O4, it contains unsaturated double bond, has active chemical property, can carry out polymerization among itself, also can polymerize with other monomers such as acrylonitrile etc., slightly soluble in benzene, chloroform, diethyl ether, petroleum ether, carbon disulfide, soluble in water, ethanol, acetone. Can carry out various addition reactions, esterification and polymerization, is important raw material of chemical synthesis industry, also is important raw material of chemical production, however, itaconic acid exists less in nature, therefore needs to prepare by fermentation method. In the process of preparing itaconic acid by fermentation method, the culture of strain using culture dish is one of key steps.

[0003] For example, the granted announcement number CN 215560273 U, a multifunctional microbial fertilizer active strain culture dish, including transparent base box, the inside of transparent base box is provided with separation assembly, the inner wall between separation assembly and transparent base box is provided with load assembly, the top of load assembly is provided with protection assembly, after the cover glass is put into load assembly, protection assembly is surrounded in the outer side of cover glass, and the object assembly is vertically lifted to facilitate the loading and unloading of cover glass, and the top of transparent base box is provided with sealing assembly;Beneficial effect is: the multifunctional microbial fertilizer active strain culture dish proposed in the utility model is provided with supporting plate between adjacent partitions to support the cover glass, and a through hole is formed in the bottom of the culture dish, the cover glass can be pushed out by inserting the finger into the through hole and pushing the supporting plate, and the elastic strip limits the rubber cover while achieving elastic clamping and limiting of the cover glass.

[0004] The traditional culture dish needs to open the dish cover when sampling, this kind of mode can influence the culture of anaerobic bacteria, and bacteria in the air can easily pollute the culture dish, therefore, the market urgently needs to develop a strain culture dish for preparing itaconic acid to help people solve the existing problems. UTILITY MODEL CONTENT

[0005] The utility model aims at providing a strain culture dish for preparing itaconic acid to solve the problem that the traditional culture dish needs to open the dish cover when sampling, which can influence the culture of anaerobic bacteria, and bacteria in the air can easily pollute the culture dish.

[0006] In order to achieve the above object, the utility model provides the following technical scheme: A preparation itaconic acid with bacterial strain culture dish, including culture dish, the upper end of culture dish is connected with dish cover, one side of upper end middle part of dish cover is provided with jack, the upper end surface of dish cover is fixedly connected with fixed tube on the upper end of jack, the inside of fixed tube is fixedly provided with sampling cup, the inside of sampling cup is provided with sampling cavity, the lower end surface middle part of sampling cup is fixedly connected with soft pipet, the upper end of sampling cavity of sampling cup and the upper end surface of sampling cup are fixedly provided with rubber plug.

[0007] Preferably, the upper end of the outer side of the culture dish is fixedly provided with a semicircular ring-shaped protrusion, and the inner side of the side end surface of the dish cover is fixedly provided with a ring-shaped sealing ring.

[0008] Preferably, the dish cover is sleeved into the upper end of the culture dish, and the semicircular ring-shaped protrusion is inserted into the inner side of the ring-shaped sealing ring.

[0009] Preferably, the upper end surface of the dish cover is fixedly provided with a sealing washer inside the fixed tube.

[0010] Preferably, the inner side end surface of the fixed tube is fixedly provided with an internal thread, and the outer side end surface of the sampling cup is fixedly provided with an external thread.

[0011] Preferably, the sampling cup and the fixed tube are fixedly connected through the internal thread and the external thread, and the lower end surface of the sampling cup is tightly attached to the upper end of the sealing washer.

[0012] Preferably, the lower end of the soft pipet extends to the lower end inside the culture dish through the middle part of the sealing washer and the jack in sequence, and the upper end of the soft pipet is communicated with the sampling cavity inside the sampling cup.

[0013] Compared with the prior art, the utility model has the beneficial effects that:

[0014] 1. In the utility model, the lower end of the soft pipet extends to the lower end inside the culture dish through the middle part of the sealing washer and the jack in sequence, and the upper end of the soft pipet is communicated with the sampling cavity inside the sampling cup.

[0015] 2. The utility model discloses a fixed pipe is provided, and the sampling cup is fixed with fixed pipe through the thread connection of inner thread part and outer thread part between sampling cup and fixed pipe, and the lower end surface of sampling cup is tightly attached with the upper end of sealing washer, so that the sampling cup is fixed with fixed pipe, and the lower end surface of sampling cup presses the upper end of sealing washer to realize the sealing of the jack when the sampling cup is fixed with fixed pipe, and the sampling cup can be detached from fixed pipe, which is convenient for the cleaning and replacement of sampling cup and its accessory components.

[0016] 3. The utility model discloses a semicircle ring convex part is provided, and the dish cover is sleeved into the upper end of petri dish and makes semicircle ring convex part insert into the inboard of annular sealing washer, makes the inboard of annular sealing washer deform after the buckling of dish cover and petri dish through semicircle ring convex part extruding into the inboard of annular sealing washer, and then makes the inboard of annular sealing washer and semicircle ring convex part tightly attached, realizes the sealing between dish cover and petri dish, prevents the bacteria in external air from entering the inside of petri dish, and guarantees the anaerobic environment of petri dish for the propagation of anaerobic bacteria at the same time. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 It is the front view of the preparation itaconic acid bacterial strain petri dish of the utility model;

[0018] Figure 2 It is the main sectional view of the utility model;

[0019] Figure 3 It is the side sectional view of the utility model;

[0020] Figure 4 It is the A detail enlarged view of the utility model.

[0021] In the drawing: 1, petri dish;101, semicircle ring convex part;2, dish cover;201, annular sealing washer;202, jack;203, sealing washer;3, fixed pipe;301, inner thread part;4, sampling cup;401, outer thread part;402, sampling cavity;403, soft pipette;404, rubber plug. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the utility model will be apparently and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments.

[0023] Please refer to Figures 1-4The utility model provides an embodiment: a preparation itaconic acid with bacterial strain culture dish, including culture dish 1, the upper end of culture dish 1 is connected with dish cover 2, the upper end middle part one side of dish cover 2 is provided with jack 202, the upper end surface of dish cover 2 is fixedly connected with fixed tube 3 on jack 202 upper end, the inside fixed setting of fixed tube 3 has sampling cup 4, the inside of sampling cup 4 is provided with sampling cavity 402, the lower end surface middle part of sampling cup 4 is fixedly connected with soft pipette 403, and the upper end between the sampling cavity 402 of sampling cup 4 and the upper end surface of sampling cup 4 is fixedly provided with rubber plug 404.

[0024] Further, the upper end of the outer side of the culture dish 1 is fixedly provided with a semicircular ring-shaped protruding portion 101, and the inner side of the side end surface of the dish cover 2 is fixedly provided with a ring-shaped sealing ring 201.

[0025] Further, the dish cover 2 is sleeved into the upper end of the culture dish 1, and the semicircular ring-shaped protruding portion 101 is inserted into the inner side of the ring-shaped sealing ring 201. After the dish cover 2 and the culture dish 1 are buckled, the semicircular ring-shaped protruding portion 101 is squeezed into the inner side of the ring-shaped sealing ring 201, the inner side of the ring-shaped sealing ring 201 is deformed, and then the ring-shaped sealing ring 201 is tightly attached to the semicircular ring-shaped protruding portion 101, the sealing between the dish cover 2 and the culture dish 1 is realized, the bacteria in the external air is prevented from entering the inside of the culture dish 1, and the anaerobic bacteria propagation in the oxygen-free environment of the culture dish 1 is ensured.

[0026] Further, the upper end surface of the dish cover 2 is fixedly provided with a sealing gasket 203 inside the fixed tube 3.

[0027] Further, the inner side of the fixed tube 3 is fixedly provided with an inner thread portion 301 on the end surface, and the outer side of the sampling cup 4 is fixedly provided with an outer thread portion 401.

[0028] Further, the sampling cup 4 and the fixed tube 3 are fixedly connected through the inner thread portion 301 and the outer thread portion 401, the lower end surface of the sampling cup 4 is tightly attached to the upper end of the sealing gasket 203, the sampling cup 4 and the fixed tube 3 are fixed, the lower end surface of the sampling cup 4 presses the upper end of the sealing gasket 203 to seal the jack 202 when the sampling cup 4 and the fixed tube 3 are fixed, the sampling cup 4 can be detached from the fixed tube 3, and the sampling cup 4 and the attached components are convenient to clean and replace.

[0029] Further, the lower end of the soft pipette 403 extends to the inside lower end of the culture dish 1 through the middle of the sealing washer 203 and the insertion hole 202 in turn, and the upper end of the soft pipette 403 communicates with the sampling cavity 402 inside the sampling cup 4. By operating the syringe, the syringe needle is inserted into the sampling cavity 402 through the rubber plug 404, the sampling cavity 402 is pulled to increase the air pressure inside, and then the bacteria liquid in the culture dish 1 is sucked into the sampling cavity 402 through the soft pipette 403, and the bacteria liquid in the sampling cavity 402 is sucked through the syringe, realizing the sampling of bacteria liquid in a completely sealed manner without opening the dish cover 2.

[0030] Working principle: when in use, first ensure that the dish cover 2 and the culture dish 1 are sealed by the close cooperation of the semicircular protruding part 101 and the annular sealing ring 201, so as to prevent external bacterial contamination and maintain an anaerobic environment. When sampling is needed, the dish cover 2 does not need to be opened, but the sampling mechanism is directly operated. The sampling mechanism is composed of a sampling cup 4, a soft pipette 403 and a rubber plug 404. The sampling cup 4 is screwed and fixed with the inner threaded part 301 of the fixed tube 3 through the outer threaded part 401, and at the same time, the lower end surface of the sampling cup 4 closely abuts the sealing washer 203 to ensure the sealing property during sampling. The lower end of the soft pipette 403 passes through the sealing washer 203 and the insertion hole 202 to enter the inside of the culture dish 1, and the upper end communicates with the sampling cavity 402 inside the sampling cup 4. When sampling, the syringe needle penetrates the rubber plug 404 to enter the sampling cavity 402, and through the negative pressure effect, the soft pipette 403 sucks the bacteria liquid in the culture dish 1 into the sampling cavity 402, and then the bacteria liquid in the sampling cavity 402 is sucked out through the syringe, completing the sampling operation. The whole process does not need to open the dish cover 2, effectively avoiding external pollution and affecting the anaerobic culture environment.

[0031] It is obvious for those skilled in the art that the present application is not limited to the details of the above exemplary embodiments, and can be realized in other specific forms without departing from the spirit or essential characteristics of the present application. Therefore, the embodiments should be regarded as exemplary and non-limiting, and the scope of the present application is defined by the appended claims rather than the above description, and all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present application. Any reference signs in the claims should not be regarded as limiting the claims involved.

Claims

1. A Petri dish for the preparation of a strain for itaconic acid production, comprising a Petri dish (1), characterised in that: The upper end of the culture dish (1) is connected with a dish cover (2), one side of the upper end of the dish cover (2) is provided with a insertion hole (202), the upper end surface of the dish cover (2) is fixedly connected with a fixed tube (3) above the insertion hole (202), the inside of the fixed tube (3) is fixedly provided with a sampling cup (4), the inside of the sampling cup (4) is provided with a sampling cavity (402), the lower end surface of the sampling cup (4) is fixedly connected with a soft straw (403), the sampling cavity (402) of the sampling cup (4) is fixedly provided with a rubber plug (404) between the upper end of the sampling cavity (402) and the upper end surface of the sampling cup (4).

2. A culture plate for preparing itaconic acid using bacterial strains according to claim 1, characterized in that: The outer side end surface of the culture dish (1) is fixedly provided with a semicircular ring-shaped convex part (101) at the upper end, and the inner side of the side end surface of the dish cover (2) is fixedly provided with a ring-shaped sealing ring (201).

3. A culture plate for preparing itaconic acid using bacterial strains according to claim 2, characterized in that: The dish cover (2) is sleeved into the upper end of the culture dish (1) and the semicircular ring-shaped convex part (101) is inserted into the inside of the ring-shaped sealing ring (201).

4. The Petri dish for preparing a strain for producing itaconic acid according to claim 1, wherein: The upper end surface of the dish cover (2) is fixedly provided with a sealing gasket (203) inside the fixed tube (3).

5. A culture plate for preparing itaconic acid using bacterial strains according to claim 4, characterized in that: The inside end surface of the fixed tube (3) is fixedly provided with an internal thread part (301), and the outside end surface of the sampling cup (4) is fixedly provided with an external thread part (401).

6. A culture plate for preparing itaconic acid using bacterial strains according to claim 5, wherein: The sampling cup (4) and the fixed tube (3) are fixedly connected through the internal thread part (301) and the external thread part (401), and the lower end surface of the sampling cup (4) is tightly attached to the upper end of the sealing gasket (203).

7. A culture plate for preparing itaconic acid using bacterial strains according to claim 6, characterized in that: The lower end of the soft straw (403) extends to the inside lower end of the culture dish (1) through the middle part of the sealing gasket (203) and the insertion hole (202) in sequence, and the upper end of the soft straw (403) is communicated with the sampling cavity (402) inside the sampling cup (4).

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