Cell culture container

Abstract

The utility model provides a cell culture container which comprises a culture bottle, a filter membrane cover is arranged on the top of one side of the culture bottle, cells needing to be cultured are placed in the culture bottle, the filter membrane cover is used for assisting in sealing, the interior of the filter membrane cover can be opened through an exhaust hole when needed, air exchange is conducted in the culture bottle, and therefore the cells can be cultured conveniently. Meanwhile, cells needing to be cultured can be placed through the cell input port, a nutrient solution needed for culturing the cells is also placed in the cell input port, and the cell input port is closed after injection is completed, so that the overall airtightness of the device is guaranteed; after the environment needed by cells is set, the temperature of the whole device can be increased through the structure of the heating assembly, it is not needed to worry that the temperature is too high to affect cell survival, meanwhile, after the heating assembly is set, the whole device is fixed through the fixing assembly, and damage to cells in a culture bottle due to some collisions is prevented; the stability of the device is further improved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to cell culture technical field, concretely relates to a cell culture container. BACKGROUND

[0002] Cell culture refers to in vitro simulation of in vivo environment (sterile, suitable temperature, pH and certain nutrition conditions etc.), makes survival, growth, reproduction and maintains the main structure and function of a method. Cell culture is also called cell cloning technology, the formal name for cell culture technology in biology. Whether for the whole biological engineering technology, or one of the biological cloning technology, cell culture is an indispensable process, cell culture is the large-scale cloning of cells. Cell culture technology can be a cell after a large number of culture becomes simple single cell or little differentiated multicellular, which is the indispensable link of cloning technology, and cell culture itself is the cloning of cells. Cell culture technology is an important and commonly used technology in cell biology research methods, through cell culture, a large number of cells can be obtained, and the signal transduction of cells, cell synthesis metabolism, cell growth and proliferation can be studied.

[0003] Cell culture usually needs container to be received and cultivated, cell culture bottle is generally used, the main function of cell culture bottle is to provide suitable environment required for cell growth, promote the growth and reproduction of cells. By simulating the physiological conditions of cells in vivo, cell culture bottle can support the growth and proliferation of microorganisms, insects or mammalian cells, but the conventional cell culture bottle cannot control temperature, some cells that need higher temperature for growth and development will be affected, and accurate cell culture data cannot be obtained. Therefore, we provide a cell culture container. UTILITY MODEL CONTENT

[0004] The utility model aims at solving the insufficient prior art, and provides a cell culture container for solving the problems in the background art.

[0005] To achieve the above object, the utility model provides the following technical scheme.

[0006] A cell culture container, comprising: a culture bottle, a filter membrane cover is arranged at the top of one side of the culture bottle, an anti-skid groove is arranged on the surface of the filter membrane cover, a cell input port is arranged at the top of the culture bottle, the position of the cell input port is matched with the inner wall of the culture bottle, a plurality of movable dismounting grooves are arranged at the bottom of the culture bottle, a heating assembly is slidably connected to the inner wall of the movable dismounting groove, and the bottom and the surface of the heating assembly are fixedly connected with the surface of the culture bottle.

[0007] Preferably, the temperature increasing assembly comprises sliding dismounting blocks, a heating water tank, a heat exhaust hole, a connecting rotating shaft and a heat conducting plate, a plurality of the sliding dismounting blocks are slidingly connected to the inner wall of the movable dismounting groove, the heating water tank is fixedly connected to the bottom of the sliding dismounting block, and the heating water tank is matched in size with the culture bottle.

[0008] Preferably, the heat exhaust hole is arranged at the top of the heating water tank, the connecting rotating shaft is arranged at the top of the heating water tank, the position of the connecting rotating shaft is matched with one side of the heat exhaust hole, the heat conducting plate is fixedly connected to one side of the connecting rotating shaft, and the heat conducting plate is matched in size with the heat exhaust hole.

[0009] Preferably, the fixing assembly comprises fixing nails, connecting plates, a gas collecting pipe, a gas exhaust pipe, a gas pressure valve and a fixing suction disc, the fixing nails are fixedly connected to the bottom and the surface of the temperature increasing assembly and the surface of the culture bottle, and the connecting plates are fixedly connected to the bottom of the fixing nail.

[0010] Preferably, the gas collecting pipe is fixedly connected to the bottom of the connecting plate, the gas exhaust pipe is fixedly connected to one side of the gas collecting pipe, the gas pressure valve is rotatably connected to one side of the gas exhaust pipe, and the fixing suction disc is fixedly connected to the bottom of the gas collecting pipe.

[0011] Preferably, the top of the filter membrane cover is provided with a gas exhaust hole.

[0012] Preferably, the culture bottle is provided with a scale on both sides, and the scale is matched in size with the culture bottle.

[0013] Compared with the prior art, the device has the following beneficial effects:

[0014] 1. The cells to be cultured are placed in the culture bottle, the filter membrane cover is used for sealing, the gas exhaust hole is used for opening the filter membrane cover to exchange the air in the culture bottle, the adaptability of the device is improved, the cells to be cultured are placed through the cell input port, the nutrient solution required for the cultured cells is also placed in the culture bottle, and the device is closed after injection, so that the airtightness of the device is ensured, the temperature of the device is increased through the structure of the temperature increasing assembly after the environment required by the cells is set, the survival of the cells is not affected by high temperature, the device is fixed through the fixing assembly after the temperature increasing assembly is set, the cells in the culture bottle are prevented from being damaged due to bumps, and the stability of the device is improved.

[0015] 2, help to provide a warm environment for the culture bottle through the overall structure of the heating assembly, use the sliding disassembly block to slide the overall heating assembly in the movable disassembly groove, facilitate the overall heating assembly to be arranged at the bottom of the culture bottle, and the heating water tank can pour hot water with a suitable water temperature according to the type of the cultured cells, the heat is introduced into the culture bottle from the heat exhaust hole and the heat conduction plate by using the temperature of the hot water, and the suitable temperature is created for the cells in the culture bottle, so that the functionality and safety of the device as a whole are further improved. BRIEF DESCRIPTION OF DRAWINGS

[0016] The accompanying drawings are used to provide a further understanding of the present application, and constitute a part of the specification, and are used to explain the present application together with embodiments of the present application, and do not constitute a limitation on the present application.

[0017] In the drawings:

[0018] Figure 1 It is an overall structure schematic view of the present application;

[0019] Figure 2 It is an overall structure back view of the present application;

[0020] Figure 3 It is an overall structure exploded view of the present application;

[0021] Figure 4 It is a schematic view of the heating assembly in the structure of the present application;

[0022] Figure 5 It is a back view of the fixing assembly in the structure of the present application.

[0023] In the drawings: 1, culture bottle; 2, filter membrane cover; 3, exhaust hole; 4, cell input port; 5, scale; 6, movable disassembly groove; 7, heating assembly; 701, sliding disassembly block; 702, heating water tank; 703, heat exhaust hole; 704, connecting shaft; 705, heat conduction plate; 8, fixing assembly; 801, fixing nail; 802, connecting plate; 803, gas collecting pipe; 804, exhaust pipe; 805, air pressure valve; 806, fixed suction cup. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0025] EMBODIMENT

[0026] Please refer toFigures 1-5 The technical scheme provided by the embodiment is as follows:

[0027] The cell culture container comprises a culture bottle 1, a filter membrane cover 2 is arranged at the top of one side of the culture bottle 1, an anti-skid groove is arranged on the surface of the filter membrane cover 2, a cell input port 4 is arranged at the top of the culture bottle 1, the position of the cell input port 4 matches the inner wall of the culture bottle 1, a plurality of movable dismounting grooves 6 are arranged at the bottom of the culture bottle 1, a heating assembly 7 is slidably connected to the inner wall of the movable dismounting groove 6, and a fixing assembly 8 is fixedly connected to the surface of the heating assembly 7 and the surface of the culture bottle 1.

[0028] In the embodiment, the culture bottle 1 can help provide a container for the development of cell culture, the bottle mouth near the filter membrane cover 2 is designed as an inclined mouth to prevent cells and nutrient solution from being discharged from the filter membrane cover 2 when the bottle is placed horizontally, further improving the stability of the device as a whole, the cell input port 4 can help the user directly inject cells and nutrient solution therefrom without opening the filter membrane cover 2, directly injecting cells in the horizontal direction, further improving the flexibility of the device as a whole, the movable dismounting groove 6 can help dismount and install the heating assembly 7, the heating assembly 7 can help heat the culture bottle 1 as a whole by using its own structure, conveniently providing a corresponding environment for the cell culture inside the culture bottle 1, further improving the adaptability of the device as a whole, and the fixing assembly 8 can be used to fix the device in a desired direction and can be easily dismounted by using the structure of the fixing assembly 8 itself, further improving the stability of the device as a whole.

[0029] The heating assembly 7 comprises sliding dismounting blocks 701, a heating water tank 702, heat discharge holes 703, a connecting rotating shaft 704 and a heat conducting plate 705, the sliding dismounting blocks 701 are slidably connected to the inner wall of the movable dismounting groove 6, the heating water tank 702 is fixedly connected to the bottom of the sliding dismounting block 701, and the size of the heating water tank 702 matches that of the culture bottle 1.

[0030] In the embodiment, the heating assembly 7 can slide into the inside of the movable dismounting groove 6 by using the sliding dismounting blocks 701, and the heating water tank 702 can be filled with water at a suitable temperature, which can help heat the bottom of the culture bottle 1 by using the water temperature, thereby slightly increasing the temperature inside the culture bottle 1, helping the cells to develop at a suitable temperature, and further improving the functionality of the device as a whole.

[0031] The heat discharge holes 703 are arranged at the top of the heating water tank 702, the connecting rotating shaft 704 is arranged at the top of the heating water tank 702, the position of the connecting rotating shaft 704 matches that of one side of the heat discharge holes 703, and the heat conducting plate 705 is fixedly connected to one side of the connecting rotating shaft 704, and the size of the heat conducting plate 705 matches that of the heat discharge holes 703.

[0032] In this embodiment, the heat exhaust hole 703 can be linked with the heat conduction plate 705 to guide the water temperature of the heating water tank 702 to the surface of the heat conduction plate 705 through the heat exhaust hole 703, and then the heat is introduced into the bottom of the culture bottle 1, so as to further improve the heat of the bottom of the culture bottle 1, and further improve the temperature of the cell culture environment in the culture bottle 1. At the same time, the heat conduction plate 705 can also prevent the hot water from spilling out and keep the temperature of the water in the heating water tank 702, and further improve the functionality of the heating assembly 7 as a whole.

[0033] The plurality of fixing assemblies 8 include fixing nails 801, connecting plates 802, air collecting pipes 803, air exhaust pipes 804, air pressure valves 805 and fixing suction cups 806. The fixing nails 801 are fixedly connected to the bottom and surface of the heating assembly 7 and the surface of the culture bottle 1. The connecting plates 802 are fixedly connected to the bottom of the fixing nails 801.

[0034] In this embodiment, the fixing assembly 8 is fixed on the bottom and surface of the heating assembly 7 and the surface of the culture bottle 1 by the fixing nails 801, so as to stabilize the fixing assembly 8 as a whole, and further improve the stability of the device as a whole.

[0035] The air collecting pipe 803 is fixedly connected to the bottom of the connecting plate 802. The air exhaust pipe 804 is fixedly connected to one side of the air collecting pipe 803. The air pressure valve 805 is rotatably connected to one side of the air exhaust pipe 804. The fixing suction cup 806 is fixedly connected to the bottom of the air collecting pipe 803.

[0036] In this embodiment, when the fixing assembly 8 is fixed on the table top by the fixing suction cup 806, the air pressure is compressed through the air collecting pipe 803, and the fixing suction cup 806 is fixed as a whole by the air pressure. When disassembly is needed, the air pressure valve 805 can be directly opened to exhaust and inhale the air from the air exhaust pipe 804, so as to exhaust the compressed air in the fixing suction cup 806. The fixing assembly 8 can be easily taken out without excessive force, which can avoid the shaking of the cells in the culture bottle 1 and the damage to the cell culture, and further improve the stability of the device as a whole.

[0037] The top of the filter membrane cover 2 is provided with an air exhaust hole 3.

[0038] In this embodiment, the air exhaust hole 3 can help the filter membrane cover 2 to directly transport oxygen into the culture bottle 1 when needed. The filter membrane cover 2 is provided with a corresponding sealing plate. When oxygen needs to be transported, the sealing plate in the filter membrane cover 2 can be taken out, and then the filter membrane cover 2 is covered, so as to transport the oxygen, or simulate the environment of the real air, so as to further improve the safety and functionality of the device as a whole.

[0039] Both sides of the culture bottle 1 are provided with a scale 5, and the size of the scale 5 matches the culture bottle 1.

[0040] In this embodiment, the scale 5 can be observed when the device is upright for storage, the growth of the nutrient solution and the whole cell, so as to further improve the functionality and accuracy of the whole device.

[0041] Working principle: the user can put the cells to be cultured into the culture bottle 1, and pour the nutrient solution to be cultured into the culture bottle 1, and use the space provided by the nutrient solution and the culture bottle 1 to provide space and nutrition for the development of the cells, further improve the functionality of the whole device, and use the filter cover 2 to help seal, and the exhaust hole 3 can open the sealing plate inside the filter cover 2 when needed, so as to exchange air inside the culture bottle 1, which can be used when the culture needs to be cultured, so as to further improve the adaptability and functionality of the whole device, and the cell input port 4 can be used to place the cells to be cultured, and the cell input port 4 can also place the nutrient solution required for the cultured cells, and close after the injection of the nutrient solution and the cells, so as to ensure the airtightness of the whole device, and after setting the environment required for the growth of the cells, the structure of the heating assembly 7 can be used to increase the temperature of the whole device, and the warm environment and sufficient nutrition can be used to ensure the culture and development of the cells, and there is no need to worry about the influence of high temperature on the survival of the cells, so as to further improve the culture effect of the whole device, and after setting the heating assembly 7, the whole device can be fixed by the fixing assembly 8, so as to prevent the cells inside the culture bottle 1 from being damaged due to some bumps, further improve the stability of the device, and when moving, the structure inside the fixing assembly 8 can be easily disassembled and moved, without worrying about the damage to the cells inside the culture bottle 1 caused by the jolt, further improving the safety of the whole device.

[0042] Although the embodiments of the present application have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to the embodiments without departing from the principles and spirits of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A cell culture vessel, characterized by: The utility model provides a culture bottle, the one side top of culture bottle (1) is provided with filter membrane cover (2), the surface of filter membrane cover (2) is provided with antiskid groove, the top of culture bottle (1) is provided with cell input port (4), the position of cell input port (4) is matched with the inner wall of culture bottle (1), the bottom of culture bottle (1) is provided with a plurality of movable dismounting groove (6), the inner wall of movable dismounting groove (6) is slidably connected with temperature rising subassembly (7), the bottom of temperature rising subassembly (7) and the surface are all fixedly connected with fixed assembly (8) with the surface of culture bottle (1).

2. A cell culture vessel as defined in claim 1, wherein: The utility model discloses a culture bottle, the one side top of culture bottle (1) is provided with filter membrane cover (2), the surface of filter membrane cover (2) is provided with antiskid groove, the top of culture bottle (1) is provided with cell input port (4), the position of cell input port (4) is matched with the inner wall of culture bottle (1), the bottom of culture bottle (1) is provided with a plurality of movable dismounting groove (6), the inner wall of movable dismounting groove (6) is slidably connected with temperature rising subassembly (7), the bottom of temperature rising subassembly (7) and the surface are all fixedly connected with fixed assembly (8) with the surface of culture bottle (1).

3. A cell culture vessel as defined in claim 2, wherein: The utility model discloses a culture bottle, the one side top of culture bottle (1) is provided with filter membrane cover (2), the surface of filter membrane cover (2) is provided with antiskid groove, the top of culture bottle (1) is provided with cell input port (4), the position of cell input port (4) is matched with the inner wall of culture bottle (1), the bottom of culture bottle (1) is provided with a plurality of movable dismounting groove (6), the inner wall of movable dismounting groove (6) is slidably connected with temperature rising subassembly (7), the bottom of temperature rising subassembly (7) and the surface are all fixedly connected with fixed assembly (8) with the surface of culture bottle (1).

4. A cell culture vessel as defined in claim 1, wherein: The utility model discloses a culture bottle, the one side top of culture bottle (1) is provided with filter membrane cover (2), the surface of filter membrane cover (2) is provided with antiskid groove, the top of culture bottle (1) is provided with cell input port (4), the position of cell input port (4) is matched with the inner wall of culture bottle (1), the bottom of culture bottle (1) is provided with a plurality of movable dismounting groove (6), the inner wall of movable dismounting groove (6) is slidably connected with temperature rising subassembly (7), the bottom of temperature rising subassembly (7) and the surface are all fixedly connected with fixed assembly (8) with the surface of culture bottle (1).

5. A cell culture vessel as defined in claim 4, wherein: The utility model discloses a culture bottle, the one side top of culture bottle (1) is provided with filter membrane cover (2), the surface of filter membrane cover (2) is provided with antiskid groove, the top of culture bottle (1) is provided with cell input port (4), the position of cell input port (4) is matched with the inner wall of culture bottle (1), the bottom of culture bottle (1) is provided with a plurality of movable dismounting groove (6), the inner wall of movable dismounting groove (6) is slidably connected with temperature rising subassembly (7), the bottom of temperature rising subassembly (7) and the surface are all fixedly connected with fixed assembly (8) with the surface of culture bottle (1).

6. A cell culture vessel as defined in claim 1, wherein: The top of filter membrane cover (2) is provided with exhaust hole (3).

7. A cell culture vessel as defined in claim 1, wherein: Both sides of culture bottle (1) are provided with scale (5), and the size of scale (5) is matched with culture bottle (1).

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