Glucose solution filling assembly for testing electrochemical biosensor and detection device

Abstract

The utility model provides a glucose solution filling assembly for testing an electrochemical biosensor and a detection device, and relates to the technical field of biosensor detection, the glucose solution filling assembly comprises a liquid storage tank, a confluence piece, a constant-temperature water bath kettle and a sensor assembling assembly, the confluence piece is provided with a liquid inlet end and a liquid outlet end, the liquid inlet end is communicated with the accommodating cavities in a time-sharing manner and is higher than the liquid outlet end, the constant-temperature water bath pot receives a glucose solution discharged from the liquid outlet end, and the sensor assembling component fixes the electrochemical biosensor; in a non-filling state, the sensor assembling component and the confluence piece are close to and far away from the constant-temperature water bath kettle respectively, and in a filling state, the sensor assembling component and the confluence piece exchange position states respectively. By optimizing the pipeline connecting structure, the problems that an existing pipeline is complex in arrangement and large in occupied space are solved, and in the filling state and the non-filling state, the problem that glucose solution filling flexibility is poor is solved through movement matching of the confluence piece and the sensor assembling assembly.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological sensor detection technical field, especially in electrochemical biosensor test glucose solution perfusion subassembly and detection device for use. BACKGROUND

[0002] Diabetes is a kind of metabolic disease with high blood sugar as the characteristic due to insufficient insulin secretion or unable to effectively utilize insulin. In order to do well in pre-diabetes prevention and its complication monitoring, it is particularly necessary to control and monitor blood sugar level, and electrochemical glucose biosensor based on glucose oxidase is widely concerned due to its advantages of high specificity and sensitivity, simple preparation, fast response speed and low cost.

[0003] In the process of batch manufacturing electrochemical glucose biosensor, the process parameters such as glucose oxidase content on the sensor, sensor area, sensor thickness, sensor outer film layer thickness are difficult to guarantee completely consistent, and the sensor is usually tested under various concentrations of glucose solution before leaving factory to screen out the sensor meeting data requirements. At present, constant temperature water bath is usually used to contain glucose solution for detection, and multiple injection ports are usually arranged on the solution tank of constant temperature water bath to perfuse different concentrations of glucose solution in time, and then a large number of electrodes of the sensor are immersed in the glucose solution.

[0004] However, multiple pipelines are usually used to communicate with the chambers storing glucose solutions with different concentrations and multiple injection ports arranged on the solution tank of constant temperature water bath in the prior art, since multiple pipelines are connected with multiple injection ports arranged fixedly, the arrangement of pipelines is required to be higher, so that the batch detection equipment is required to have larger installation space, so that the whole equipment is large. At the same time, the multiple injection ports arranged fixedly cannot realize linkage, and the glucose solutions with different concentrations can only be injected into the solution tank of constant temperature water bath from the corresponding injection ports, which affects the flexibility of the injection mode of glucose solutions with different concentrations, and cannot guarantee that residual liquid exists between the injection ports, which easily leads to slight change of the concentration of glucose solution, thereby affecting the test precision of electrochemical glucose biosensor. UTILITY MODEL CONTENTS

[0005] Therefore, the utility model aims at providing a kind of electrochemical biosensor test glucose solution perfusion subassembly and detection device, when electrochemical glucose biosensor is detected, by optimizing pipeline connection structure and setting the movement cooperation relationship of relevant components, the purpose of high-efficiency delivery of glucose solution to constant temperature water bath is achieved.

[0006] To achieve the above-mentioned purpose, the utility model adopts the following technical solutions:

[0007] The utility model provides a glucose solution perfusion assembly for electrochemical biosensor test, including liquid storage tank, confluence spare, constant temperature water -bath, sensor assembly component, liquid storage tank is equipped with the accommodation cavity of different concentration glucose solution is filled, confluence spare has liquid inlet end and liquid outlet end, liquid inlet end is connected with each accommodation cavity in time, liquid inlet end is higher than liquid outlet end, constant temperature water -bath is used for the glucose solution that is discharged from liquid outlet end is accepted, and sensor assembly component is used for fixed electrochemical biosensor, wherein, when glucose solution perfusion assembly is in non perfusion state, sensor assembly component is close to constant temperature water -bath until the reaction end of electrochemical biosensor is immersed into glucose solution, and confluence spare is far from constant temperature water -bath, when glucose solution perfusion assembly is in perfusion state, sensor assembly component is far from constant temperature water -bath until the reaction end of electrochemical biosensor is separated from glucose solution, and confluence spare is close to constant temperature water -bath until liquid outlet end is located above the area of constant temperature water -bath and is accepted glucose solution.

[0008] In addition, the glucose solution perfusion assembly for electrochemical biosensor test according to the utility model can also have the following additional technical features:

[0009] Further, the glucose solution perfusion assembly comprises a first driving mechanism, which is used to drive the confluence spare to move towards or away from the constant temperature water-bath. The first driving mechanism comprises a sliding rail, a sliding block and a driver. The sliding rail is arranged beside the constant temperature water-bath. The sliding block is slidingly arranged on the sliding rail. The confluence spare is arranged on the sliding block. The driver is in transmission connection with the sliding block, so as to push the sliding block to move on the sliding rail towards or away from the constant temperature water-bath.

[0010] Further, the liquid storage tank comprises a tank body and a first heating assembly. The tank body is provided with a first heating cavity for containing a heat transfer medium. The accommodation cavities are arranged in the first heating cavity. The first heating assembly is arranged in the first heating cavity and is used to heat the heat transfer medium in the first heating cavity.

[0011] Further, the liquid storage tank comprises a first internal circulation water treatment assembly, which is used to circulate and guide the heat transfer medium in the first heating cavity. The first internal circulation water treatment assembly comprises a first circulation water pump, a first shunt and a second shunt. The first circulation water pump is arranged in the first heating cavity or outside the first heating cavity. The first circulation water pump has a first suction inlet and a first discharge outlet. The first shunt is arranged in the first heating cavity. The first shunt has a plurality of first water inlets and one first water outlet, which are dispersed in the first heating cavity. The first water outlet is in communication with the first suction inlet. The second shunt is arranged in the first heating cavity. The second shunt has one second water inlet and a plurality of second water outlets, which are dispersed in the first heating cavity. The second water inlet is in communication with the first discharge outlet.

[0012] Further, the first heating cavity is in communication with the confluence spare through an openable and closable pipeline assembly.

[0013] Further, the constant-temperature water bath kettle comprises a kettle body, a tank pool and a second heating assembly, the kettle body is provided with a second heating cavity for containing a heat transfer medium, the tank pool is arranged on the kettle body and is at least partially immersed in the heat transfer medium in the second heating cavity, and the second heating assembly is arranged in the second heating cavity and used for heating the heat transfer medium in the second heating cavity.

[0014] Further, the constant-temperature water bath kettle comprises a second internal circulation water treatment assembly used for circulating and guiding the heat transfer medium in the second heating cavity, the second internal circulation water treatment assembly comprises a second circulation water pump, a third shunt and a fourth shunt, the second circulation water pump is arranged in the second heating cavity or outside the second heating cavity, the second circulation water pump is provided with a second suction inlet and a second discharge outlet, the third shunt is arranged in the second heating cavity, the third shunt is provided with a plurality of third water inlets and a third water outlet, the third water outlet is communicated with the second suction inlet, and the fourth shunt is arranged in the second heating cavity, the fourth shunt is provided with a fourth water inlet and a plurality of fourth water outlets, and the fourth water inlet is communicated with the second discharge outlet.

[0015] Further, the sensor assembly component comprises a mounting table and a sensor sealing module, a plug-in area is arranged in the middle of the mounting table, the plug-in area is provided with a plug-in groove, the sensor sealing module is plugged into the plug-in groove, and the sensor sealing module is provided with a fixing portion used for fixing the electrochemical biological sensor at one end close to the constant-temperature water bath kettle.

[0016] Further, the sensor assembly component comprises a second driving mechanism, and the second driving mechanism is in transmission connection with the mounting table, so as to drive the mounting table to be close to or away from the constant-temperature water bath kettle.

[0017] In the second aspect, the utility model also provides a kind of detection device, including the glucose solution perfusion component of electrochemical biological sensor test of preceding description, and signal acquisition component, control processing unit;Wherein, signal acquisition component is used to collect the electrical signal generated by electrochemical biological sensor and glucose solution reaction;Control processing unit is used to control the operating state of liquid storage tank and constant-temperature water bath kettle, and the signal collected by signal acquisition component is handled.

[0018] The utility model has at least the following beneficial effects: different concentrations of glucose solution are injected into the constant-temperature water bath kettle through the current collector at different times, which can reduce the complexity of pipeline arrangement, reduce the occupied space of the pipeline and reduce the volume of the whole machine equipment; at the same time, in the perfusion and non-perfusion state, through the movement cooperation of the current collector and the sensor assembly component, the problem of poor flexibility of glucose solution perfusion can be solved when safe perfusion operation is realized; in addition, the current collector shares an outlet channel with each pipeline, and is inclinedly arranged, which can ensure that the pipelines on each input side of the current collector are drained clean and reduce the degree of mutual interference of glucose solutions of various concentrations. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall assembly of an embodiment of the present utility model;

[0020] Figure 2 This is a schematic diagram of the liquid storage tank in one embodiment of the present invention;

[0021] Figure 3 This is a schematic diagram of the busbar connection structure in one embodiment of the present utility model;

[0022] Figure 4 This is a cross-sectional view of the liquid storage tank in one embodiment of the present invention;

[0023] Figure 5 This is a top view of the liquid storage tank in one embodiment of the present invention;

[0024] Figure 6 This is a schematic diagram of the structure of the tank in one embodiment of the present invention;

[0025] Figure 7 This is a schematic diagram of the structure of the second heating cavity in one embodiment of the present invention;

[0026] Figure 8 This is an exploded view of a constant temperature water bath in one embodiment of the present invention.

[0027] Figure 9 This is an overall assembly drawing of the sensor sealing module in one embodiment of the present invention;

[0028] Figure 10 This is an exploded view of the sensor sealing module in one embodiment of the present invention;

[0029] Figure 11 This is a first-view cross-sectional view of the sensor sealing module in one embodiment of the present invention;

[0030] Figure 12 This is a cross-sectional view from a second perspective of the sensor sealing module in one embodiment of the present invention;

[0031] Figure 13 This is a top view of the mounting platform in one embodiment of the present utility model;

[0032] Figure 14 This is a front view of the mounting platform in one embodiment of the present invention;

[0033] Figure 15 This is a side view of the mounting platform in one embodiment of the present invention;

[0034] Explanation of key component symbols:

[0035] Frame 10, collection box 20

[0036] Liquid storage tank 100, first tank body 110, first heating cavity 111, second tank body 120, accommodating cavity 121, first heating assembly 130, first internal circulation water treatment assembly 140, first circulation water pump 141, first shunt 142, third tank body 170

[0037] Converging piece 200, injection port 210, discharge port 220

[0038] First driving mechanism 300, sliding rail 310, sliding block 320, driver 330

[0039] Constant temperature water bath pot 400, pot body 410, second heating cavity 411, heat preservation layer 412, tank pool 420, second heating assembly 430, second internal circulation water treatment assembly 440, second circulation water pump 441, second shunt 442

[0040] Sensor assembly component 500, mounting table 510, slot 511, sensor sealing module 520, shell 521, pressing plate 5211, upper cover 5212, lower cover 5213, mounting cavity 5214, positioning groove 5215, jack 5216, signal acquisition component 522, second driving mechanism 530

[0041] Control processing unit 600, PLC unit 610, upper computer unit 620, data acquisition and display unit 630

[0042] Electrochemical biosensor 700

[0043] Liquid inlet 112, on-off valve 122, temperature sensor 150, exhaust port 160, overflow port 421, sealing member 540, induction sensor 800, liquid level sensor 900

[0044] The following specific embodiments will further illustrate the present application in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0045] In order to facilitate the understanding of the present application, the present application will be described more fully below with reference to the related drawings. Several embodiments of the present application are shown in the drawings. However, the present application can be realized in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present application more thorough and comprehensive.

[0046] It should be noted that when an element is referred to as being "on" another element, it can be directly on the other element or intervening elements can also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements can also be present. As used herein the terms "vertical", "horizontal", "left", "right" and similar terms are used for explanation purposes only.

[0047] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used in the description of the application herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed items.

[0048] Reference will now be made to the drawings, wherein Figures 1 to 15 The application provides a glucose solution perfusion assembly for electrochemical biosensor testing, which comprises a liquid storage tank 100, a current collecting piece 200, a constant temperature water bath 400 and a sensor assembly 500.

[0049] Specifically, the liquid storage tank 100 is provided with accommodating cavities 121 for containing glucose solutions with different concentrations, for example, seven accommodating cavities 121 for containing 0 mmol / L, 5 mmol / L, 10 mmol / L, 15 mmol / L, 20 mmol / L, 25 mmol / L and 30 mmol / L glucose solutions. The flow collector 200 has a liquid inlet end and a liquid outlet end, and the liquid inlet end of the flow collector 200 is in communication with each of the accommodating cavities 121. When a glucose solution with a certain concentration is tested, the flow channel between the corresponding accommodating cavity 121 and the liquid inlet end is opened. In order to reduce the risk of residual glucose solution in the flow channel between each accommodating cavity 121 and the liquid inlet end, the liquid inlet end of the flow collector 200 is arranged to be higher than the liquid outlet end, so that the glucose solution in the flow channel can be completely discharged from the liquid outlet end under the action of gravity. For example, the flow collector 200 adopts a flow collector bar, one side of the flow collector bar is provided with seven injection ports 210, the other side of the flow collector bar is provided with one discharge port 220, the seven injection ports 210 are connected to the seven accommodating cavities 121 through conduits, and the flow collector bar is arranged to be inclined, so that the seven injection ports 210 of the flow collector bar are all higher than the discharge port 220. When one of the injection ports 210 is in communication with the corresponding accommodating cavity 121, the glucose solution in the accommodating cavity 121 can be discharged from the discharge port 220 along the flow channel, and when the flow channel is closed, the residual glucose solution in the accommodating cavity 121 can also be completely discharged from the discharge port 220 along the flow channel. The constant temperature water bath 400 is used to receive the glucose solution discharged from the liquid outlet end of the flow collector 200, and the sensor assembly 500 is used to fix the electrochemical biosensor 700 during testing, so as to facilitate the immersion and removal of the electrochemical biosensor 700 in the glucose solution contained in the constant temperature water bath 400.

[0050] When the glucose solution perfusion assembly is in a non-perfusion state, the sensor assembly 500 is close to the constant temperature water bath 400, until the reaction end of the electrochemical biosensor 700 is immersed in the glucose solution, and at the same time, in order to avoid the position interference between the flow collector 200 and the sensor assembly 500, the flow collector 200 is away from the constant temperature water bath 400. In this way, the electrochemical biosensor 700 can safely react with the glucose solution in the constant temperature water bath 400.

[0051] When the glucose solution perfusion assembly is in a perfusion state, the flow collector 200 is close to the constant temperature water bath 400, until the liquid outlet end is above the area of the constant temperature water bath 400 for receiving the glucose solution, and at the same time, in order to avoid the position interference between the sensor assembly 500 and the flow collector 200, the sensor assembly 500 is away from the constant temperature water bath 400, until the reaction end of the electrochemical biosensor 700 is out of the glucose solution. In this way, the glucose solution in the corresponding accommodating cavity 121 can be discharged into the constant temperature water bath 400 through the flow collector 200.

[0052] Optionally, in the present embodiment, a switch valve 122a can be arranged on the liquid storage tank 100, and the flow passage between the corresponding accommodating cavity 121 and the liquid inlet end can be turned on or turned off by controlling the on-off of the current of the switch valve 122a.

[0053] In some optional embodiments, as shown in Figure 3 The electrochemical biosensor test glucose solution perfusion assembly further comprises a first driving mechanism 300 for driving the flow converging piece 200 to move towards or away from the constant temperature water bath 400, so that the liquid outlet end of the flow converging piece 200 is away from and close to the constant temperature water bath 400.

[0054] In some optional embodiments, as shown in Figure 3 The first driving mechanism 300 comprises a sliding rail 310, a sliding block 320, and a driver 330. The sliding rail 310 is fixedly installed on one side of the constant temperature water bath 400, the sliding block 320 is slidingly arranged on the sliding rail 310, the flow converging piece 200 is fixedly arranged on the sliding block 320, and the driver 330 is in transmission connection with the sliding block 320. When the driver 330 works, it can push the sliding block 320 to reciprocatingly move on the sliding rail 310, so that the flow converging piece 200 is away from and close to the constant temperature water bath 400. Optionally, the driver 330 can be an electric cylinder or a pneumatic cylinder.

[0055] In some optional embodiments, as shown in Figure 3 In order to avoid that the flow converging piece 200 has not completely moved away from the constant temperature water bath 400 and hinders the movement process of the reaction end of the electrochemical biosensor 700 being immersed in the constant temperature water bath 400, an inductive sensor 800a for detecting the position of the sliding block 320 can be arranged on the movement path of the flow converging piece 200. When the sliding block 320 is detected by the inductive sensor 800a, the control sensor assembly 500 starts to move the electrochemical biosensor 700.

[0056] Since the glucose solution in the accommodating cavity 121 enters the constant temperature water bath 400 from the liquid outlet end of the flow converging piece 200, large water splashes and ripples can be generated, which can affect the accuracy of the glucose solution height in the constant temperature water bath 400 collected by the liquid level sensor 800a. Therefore, in some optional embodiments, as shown in Figure 1 The liquid outlet end of the flow converging piece 200 is arranged in a flat wide mouth shape, so that the glucose solution discharged from the liquid outlet end of the flow converging piece 200 flows smoothly, and the impact force on the glucose solution in the constant temperature water bath 400 is small, thereby avoiding the generation of large water splashes and ripples.

[0057] In some optional embodiments, as shown in Figures 2 to 5As shown, the liquid storage tank 100 comprises a first tank body 110, a second tank body 120, and a first heating assembly 130. The first tank body 110 is provided with a first heating cavity 111 for containing a heat transfer medium. The second tank body 120 is provided with a plurality of second tank bodies 120, each of which is arranged in the first heating cavity 111. The second tank body 120 is internally formed with a containing cavity 121 for containing a glucose solution of a corresponding concentration. The first heating assembly 130 is arranged in the first heating cavity 111 and heats the heat transfer medium in the first heating cavity 111 when in operation. The heated heat transfer medium conducts heat to the glucose solution in the second tank body 120. Optionally, the first heating assembly 130 can be an electric heating rod.

[0058] In this embodiment, the first heating assembly 130 preheats the glucose solutions of different concentrations for detection to a temperature close to or reaching the detection requirement. This can quickly reach the use temperature condition during detection, thereby reducing the heating time during switching of different glucose solutions and improving the detection efficiency.

[0059] In some optional embodiments, the heat transfer medium in the first heating cavity 111 is an aqueous solution. In order to enable the heated heat transfer medium to uniformly conduct heat to the glucose solution in the second tank body 120, the first heating assembly 130 can be arranged in the first heating cavity 111. Figure 2 As shown, the liquid storage tank 100 further comprises a first internal circulating water treatment assembly 140. When in operation, the first internal circulating water treatment assembly 140 circulates and guides the heat transfer medium in the first heating cavity 111, so that the heat transfer medium in the first heating cavity 111 is uniformly distributed in heat, thereby improving the uniformity of heat exchange between the glucose solution in the second tank body 120 and the heat transfer medium.

[0060] In some optional embodiments, as shown in Figure 2 The first internal circulating water treatment assembly 140 comprises a first circulating water pump 141 provided with a plurality of inlet pipelines and a plurality of outlet pipelines. The first circulating water pump 141 respectively sucks in water from each corner of the first heating cavity 111 through the plurality of inlet pipelines, and simultaneously uniformly disperses and sends the water to each corner of the first heating cavity 111 through the plurality of outlet pipelines. In this way, the heat transfer medium in the first heating cavity 111 can be uniformly distributed in heat. Optionally, the first circulating water pump 141 can be arranged in the first heating cavity 111 or on the outside of the first tank body 110.

[0061] In some optional embodiments, as shown in Figure 2As shown, the first inner circulating water treatment assembly 140 further comprises two groups of first shunt 142a, one group of first shunt 142 has multiple inlets and one outlet, the outlet of the first shunt 142 is communicated with the inlet of the first circulating water pump 141, and the other group of first shunt 142 has multiple outlets and one inlet, the inlet of the first shunt 142 is communicated with the outlet of the first circulating water pump 141. In operation, the first circulating water pump 141 sucks water from each corner in the first heating cavity 111 through one group of first shunt 142, and at the same time, the first circulating water pump 141 uniformly disperses the water into each corner in the first heating cavity 111 through the other group of first shunt 142, so that the heat distribution of the heat transfer medium in the first heating cavity 111 is uniform.

[0062] In some optional embodiments, as shown in FIG. 1, the first heating cavity 111 is connected with the first circulating water pump 141 through a pipe assembly. The pipe assembly can be a pipe assembly with a switch valve 122b, or a pipe assembly without a switch valve 122b. Figures 1 to 3 As shown, the first heating cavity 111 is communicated with the current collector 200 through a pipe assembly that can be opened and closed. Alternatively, a switch valve 122b can be provided on the liquid storage tank 100 and connected with an interface (not shown in the figure) provided on the current collector 200 through a pipe, or a plurality of pipes can be used to connect different injection ports 210 provided on the current collector 200, and finally the flow passage between the first heating cavity 111 and the liquid inlet end is turned on or turned off by controlling the current on-off of the switch valve 122b. In this way, during the switching process of different glucose solutions, the heat transfer medium in the first heating cavity 111 can be guided into the current collector 200 to clean the internal flow passage of the current collector 200, so as to avoid the influence of residual glucose solution on the detection accuracy, and at the same time, the heat transfer medium in the first heating cavity 111 can be guided into the constant temperature water bath 400 through the current collector 200 to clean the constant temperature water bath 400, so as to avoid the influence of residual glucose solution in the constant temperature water bath 400 on the detection accuracy. Specifically, the tank 420 on the constant temperature water bath 400 is cleaned to avoid the influence of residual glucose solution in the tank 420 on the detection accuracy.

[0063] In some optional embodiments, in order to prevent the heat transfer medium in the first heating cavity 111 from evaporating, the first heating cavity 111 is hermetically sealed. Further, in order to avoid the influence of the external environment on the temperature of the heat transfer medium in the first heating cavity 111, the first heating cavity 111 can be provided with heat preservation, for example, heat insulation cotton or other heat preservation fillers are provided on the outside and the opening and closing part of the first heating cavity 111.

[0064] In some optional embodiments, in order to prevent the glucose solution in the accommodating cavity 121 from evaporating and affecting its concentration, the accommodating cavity 121 is hermetically sealed. Further, in order to avoid the influence of the external environment on the temperature of the glucose solution in the accommodating cavity 121, the accommodating cavity 121 can be provided with heat preservation, for example, heat insulation cotton or other heat preservation fillers are provided on the opening and closing part of the accommodating cavity 121.

[0065] In some optional embodiments, as shown in Figure 5 The first heating cavity 111 is provided with a temperature sensor 150a, through which the temperature of the heat transfer medium in the first heating cavity 111 can be measured in real time. Optionally, multiple temperature sensors 150a can be provided, so that the temperature distribution of each region in the first heating cavity 111 can be collected. When the temperature does not meet the requirements, the first heating assembly 130 is controlled to continue heating until the temperature of the heat transfer medium in the first heating cavity 111 meets the detection requirements.

[0066] In some optional embodiments, as shown in Figure 4 The first heating cavity 111 is provided with a liquid level sensor 900a, through which the height of the heat transfer medium in the first heating cavity 111 can be measured in real time. Optionally, the liquid level sensor 900a can be a high-precision ultrasonic liquid level sensor. When the height of the heat transfer medium in the first heating cavity 111 is detected to be at a low level, the control device stops detection so as to carry out the operation of supplementing the heat transfer medium. Specifically, a liquid filling port 112a can be provided on the first tank body 110, through which the heat transfer medium is supplemented.

[0067] In some optional embodiments, as shown in Figure 4 and Figure 5 Each accommodation cavity 121 is provided with a liquid level sensor 900b, through which the height of the glucose solution in the accommodation cavity 121 can be measured in real time. Optionally, the liquid level sensor 900b can be a high-precision ultrasonic liquid level sensor. When the height of the glucose solution in the accommodation cavity 121 is detected to be at a low level, the control device stops detection so as to carry out the operation of supplementing the glucose solution. Specifically, a liquid filling port 112b can be provided on the accommodation cavity 121, through which the glucose solution of the corresponding concentration is supplemented.

[0068] In some optional embodiments, as shown in Figure 4 and Figure 5 Each accommodation cavity 121 is provided with a temperature sensor 150b, through which the temperature of the glucose solution in the accommodation cavity 121 can be measured in real time. When the temperature does not meet the requirements, the first heating assembly 130 is controlled to continue heating until the temperature of the heat transfer medium in the first heating cavity 111 meets the requirements.

[0069] In some optional embodiments, as shown in Figure 4 and Figure 5 The accommodation cavity 121 is provided with an exhaust port 160b, through which the atmospheric pressure can be balanced, facilitating the delivery of the glucose solution in the accommodation cavity 121.

[0070] In some optional embodiments, as shown in Figure 4 and Figure 5 The liquid tank 100 includes a third tank 170, which is arranged in the first heating cavity 111 and used for temporarily storing cleaning liquid, such as purified water or deionized water, for cleaning the flow combining member 200.

[0071] In some optional embodiments, as shown in Figure 5 The third tank 170 is provided with a liquid level sensor 900c, by which the height of the cleaning liquid in the third tank 170 can be measured in real time. Optionally, the liquid level sensor 900c can be a high-precision ultrasonic liquid level sensor. When the cleaning liquid in the accommodating cavity 121 is detected to be at a low liquid level, the control device is stopped for performing a cleaning liquid supplementing operation. Specifically, a liquid supplementing opening 112c can be arranged on the third tank 170, through which the cleaning liquid is supplemented.

[0072] In some optional embodiments, as shown in Figure 5 The third tank 170 is provided with a temperature sensor 150c, by which the temperature of the cleaning liquid in the third tank 170 can be measured in real time. When the temperature does not meet the requirement, the first heating assembly 130 is controlled to continuously heat until the temperature of the heat transfer medium in the first heating cavity 111 meets the requirement.

[0073] In some optional embodiments, as shown in Figure 5 The third tank 170 is provided with an exhaust port 160c, through which the atmospheric pressure can be balanced, facilitating the delivery of the cleaning liquid in the third tank 170.

[0074] In some optional embodiments, as shown in Figures 6 to 8 The constant-temperature water bath pot 400 includes a pot body 410, a tank pool 420 and a second heating assembly 430. The pot body 410 is provided with a second heating cavity 411 for containing heat transfer medium. The tank pool 420 is arranged on the pot body 410 and at least partially immersed in the heat transfer medium in the pot body 410, so that the heat of the heat transfer medium in the pot body 410 can be transferred to the tank pool 420. In order to improve the heat transfer efficiency, preferably, the side wall and the bottom of the tank pool 420 are all immersed in the heat transfer medium in the second heating cavity 411. The second heating assembly 430 is arranged in the second heating cavity 411. When the second heating assembly 430 works, it heats the heat transfer medium in the second heating cavity 411. Thus, the heat transfer medium heated by the second heating assembly 430 in the second heating cavity 411 can conduct heat to the glucose solution contained in the tank pool 420, so that the temperature of the glucose solution contained in the tank pool 420 is maintained at a detected required temperature. Optionally, the second heating assembly 430 can be an electric heating rod.

[0075] In some optional embodiments, the heat transfer medium in the second heating chamber 411 is an aqueous solution. To ensure that the heated heat transfer medium can uniformly transfer heat to the glucose solution in the tank 420, such as... Figure 8 As shown, the constant temperature water bath 400 also includes a second internal circulation water treatment component 440. When the second internal circulation water treatment component 440 is working, it circulates and guides the heat transfer medium in the second heating chamber 411, so that the heat in the heat transfer medium in the second heating chamber 411 is evenly distributed, and the glucose solution in the tank 420 is also heated evenly.

[0076] In some alternative embodiments, such as Figure 5 and Figure 7 As shown, in order to reduce the heating time of the glucose solution in the tank 420, the first heating chamber 111 is connected to the second heating chamber 411 through an openable and closable piping assembly. Optionally, a switch valve 122c can be installed on the pipeline between the first heating chamber 111 and the second heating chamber 411, and the flow channel between the first heating chamber 111 and the second heating chamber 411 can be opened or closed by controlling the current of the switch valve 122c.

[0077] In this embodiment, since the temperature of the heat transfer medium in the first heating chamber 111 is close to the temperature required for detection, when the heat transfer medium in the first heating chamber 111 is transported to the second heating chamber 411, the heating time of the heat transfer medium in the second heating chamber 411 can be reduced, thereby improving the detection efficiency.

[0078] In some optional embodiments, to reduce the heating time of the glucose solution in the tank 420, the first tank 110 is connected to the second heating chamber 411 via an openable and closable piping assembly. Optionally, a switching valve 122c can be installed on the pipeline between the first heating chamber 111 and the second heating chamber 411, and the flow channel between the first heating chamber 111 and the second heating chamber 411 can be opened or closed by controlling the current of the switching valve 122c.

[0079] In this embodiment, since the temperature of the heat transfer medium in the first heating chamber 111 is close to the temperature required for detection, when the heat transfer medium in the first heating chamber 111 is transported to the second heating chamber 411, the heating time of the heat transfer medium in the second heating chamber 411 can be reduced, thereby improving the detection efficiency.

[0080] In some alternative embodiments, such as Figure 8As shown, in order to ensure the accuracy of the amount of glucose solution added to the constant temperature water bath 400, a liquid level sensor 900d can be installed on the tank 420, through which the height of the glucose solution in the tank 420 can be measured in real time. Optionally, the liquid level sensor 900d can be a high-precision ultrasonic liquid level sensor.

[0081] In some optional embodiments, as shown in Figure 6 As shown, in order to prevent the added glucose solution from overflowing the tank 420 when the liquid level sensor 900d is damaged, an overflow port 421a is provided on the tank 420, and whether the glucose solution can be stopped from being added can be determined by observing whether the glucose solution overflows the overflow port 421a.

[0082] In some optional embodiments, as shown in Figure 6 As shown, a temperature sensor 150d is provided in the tank 420, through which the temperature of the glucose solution in the tank 420 can be measured in real time. When the temperature does not meet the requirements, the second heating assembly 430 is controlled to continue heating until the temperature of the heat transfer medium in the second heating cavity 411 meets the requirements.

[0083] In some optional embodiments, as shown in Figure 8 As shown, the second internal circulating water treatment assembly 440 includes a second circulating water pump 441, which is provided with a plurality of inlet pipes and a plurality of outlet pipes. The second circulating water pump 441 sucks water from each corner of the second heating cavity 411 through the plurality of inlet pipes, and simultaneously, the second circulating water pump 441 uniformly disperses the water into each corner of the second heating cavity 411 through the plurality of outlet pipes, so that the heat of the heat transfer medium in the second heating cavity 411 is uniformly distributed. Optionally, the second circulating water pump 441 can be arranged inside the second heating cavity 411 or outside the pot body 410.

[0084] In some optional embodiments, as shown in Figure 1 As shown, the second internal circulating water treatment assembly 440 further includes two groups of second shunt rows 442. One group of the second shunt rows 442 has a plurality of inlets and an outlet, and the outlet of the second shunt row 442 is in communication with the inlet of the second circulating water pump 441. The other group of the second shunt rows 442 has a plurality of outlets and an inlet, and the inlet of the second shunt row 442 is in communication with the outlet of the second circulating water pump 441. When working, the second circulating water pump 441 sucks water from each corner of the second heating cavity 411 through one group of the second shunt rows 442, and simultaneously, the second circulating water pump 441 uniformly disperses the water into each corner of the second heating cavity 411 through the other group of the second shunt rows 442, so that the heat of the heat transfer medium in the second heating cavity 411 is uniformly distributed.

[0085] In some optional embodiments, as shown in Figure 7 In order to ensure that the second heating cavity 411 can be filled, an overflow port 421b is arranged on the second heating cavity 411 when the heat transfer medium is filled into the second heating cavity 411. At the same time, in order to stop filling the heat transfer medium in time, an inductive sensor 800b is installed at the overflow port 421b, and whether the second heating cavity 411 is full can be detected through the inductive sensor 800b.

[0086] In some optional embodiments, as shown in Figure 7 A temperature sensor 150e is arranged in the second heating cavity 411, and the temperature of the heat transfer medium in the second heating cavity 411 can be measured in real time through the temperature sensor 150e. Optionally, a plurality of temperature sensors 150e can be arranged, so that the temperature distribution of each region in the second heating cavity 411 can be collected. When the temperature does not meet the requirements, the temperature of the heat transfer medium in the second heating cavity 411 can be accurately adjusted by controlling the second heating assembly 430.

[0087] In some optional embodiments, as shown in Figure 6 In order to facilitate the discharge of the glucose solution in the tank pool 420, a discharge port that can be opened and closed is arranged on the tank pool 420. Optionally, a switch valve 122d can be arranged on the tank pool 420, and the discharge port on the tank pool 420 can be turned on by controlling the on-off of the current of the switch valve 122d.

[0088] In some optional embodiments, in order to facilitate the injection of the heat transfer medium into the second heating cavity 411, an injection port that can be opened and closed is arranged on the pot body 410 (not shown in the figure). Optionally, a switch valve can be arranged on the pot body 410, and the injection port on the pot body 410 can be turned on or cut off by controlling the on-off of the current of the switch valve.

[0089] In some optional embodiments, in order to facilitate the discharge of the heat transfer medium in the second heating cavity 411, a discharge port that can be opened and closed is arranged on the tank pool 420 (not shown in the figure). Optionally, a switch valve can be arranged on the pot body 410, and the discharge port on the pot body 410 can be turned on or cut off by controlling the on-off of the current of the switch valve.

[0090] In some optional embodiments, as shown in Figure 7 A heat preservation layer 412 is arranged on the circumferential side of the pot body 410. By arranging the heat preservation layer 412, the temperature of the glucose solution in the tank pool 420 and the temperature of the heat transfer medium in the second heating cavity 411 can be prevented from being affected by the external environment.

[0091] In some optional embodiments, as shown in Figures 9 to 15As shown, the sensor assembly 500 comprises a mounting table 510 and a sensor sealing module 520, the mounting table 510 is provided with a plug-in area in the middle, the plug-in area is provided with a slot 511, the sensor sealing module 520 is plugged into the slot 511, and the sensor sealing module 520 is provided with a fixing portion for fixing the electrochemical biosensor 700 at one end close to the constant temperature water bath 400.

[0092] In some optional embodiments, the sensor assembly 500 further comprises a second driving mechanism 530, which is in driving connection with the mounting table 510 to drive the mounting table 510 to approach or move away from the constant temperature water bath 400. Optionally, the second driving mechanism 530 can be a combination of a servo motor, an encoder and a linear module, so as to realize accurate control of the position of the sensor sealing module 520.

[0093] In a second aspect, the utility model also provides a kind of detection device, comprising the glucose solution perfusion assembly for electrochemical biosensor test of preceding description, and signal acquisition component 522, control processing unit 600;Wherein, signal acquisition component 522 is used to collect the electrical signal generated by the reaction of electrochemical biosensor 700 and glucose solution;Control processing unit 600 is used to control the operating state of liquid storage tank 100 and constant temperature water bath 400, and the signal collected by signal acquisition component 522 is handled.

[0094] Specifically, in batch detection, the control processing unit 600 controls the second driving mechanism 530 to drive the mounting table 510 to approach the constant temperature water bath 400, until the reaction end of the electrochemical biosensor 700 fixed on the sensor sealing module 520 is immersed in the glucose solution contained in the tank pool 420. At this time, the electrical signal collected by the signal acquisition component 522 is transmitted to the control processing unit 600 for analysis and processing. At the same time, in order to avoid the interference of the current collector 200 with the position of the sensor assembly 500, the control processing unit 600 also controls the first driving mechanism 300 to drive the current collector 200 to move away from the constant temperature water bath 400. When filling the glucose solution into the tank pool 420, the control processing unit 600 controls the second driving mechanism 530 to drive the mounting table 510 to move away from the constant temperature water bath 400, and at the same time, the control processing unit 600 also controls the first driving mechanism 300 to drive the current collector 200 to approach the constant temperature water bath 400, until the liquid outlet end is located above the glucose solution receiving area of the constant temperature water bath 400, so that the glucose solution in the corresponding accommodation cavity 121 can be discharged to the constant temperature water bath 400 through the current collector 200.

[0095] In some optional embodiments, the sensor sealing module 520 comprises a housing 521, the housing 521 is provided with a fixing portion for fixing the electrochemical biosensor 700 at one end close to the constant temperature water bath 400, and the signal acquisition component 522 is sealingly arranged in the housing 521.

[0096] Specifically, the shell 521 comprises a pressing plate 5211, an upper cover 5212 and a lower cover 5213, and the upper cover 5212 and the lower cover 5213 can be sealed together. When the upper cover 5212 and the lower cover 5213 are combined, a sealed mounting cavity 5214 is formed between the two, and the signal acquisition assembly 522 is arranged in the mounting cavity 5214. The side of the lower cover 5213 away from the upper cover 5212 is provided with a positioning groove 5215 for fixing the pressing plate 5211, and is provided with a jack 5216 communicating the mounting cavity 5214 and the positioning groove 5215.

[0097] The signal acquisition assembly 522 comprises an electrically connected circuit board 5221 and a conductive compression member 5222, the circuit board 5221 is arranged in the mounting cavity 5214, and the conductive compression member 5222 is arranged in the jack 5216, one end of the conductive compression member 5222 extends into the mounting cavity 5214 and is electrically connected with the circuit board 5221, the other end of the conductive compression member 5222 extends into the positioning groove 5215 and is connected with the electrochemical biosensor 700, and then the pressing plate 5211 is positioned and assembled in the positioning groove 5215, so that the electrochemical biosensor 700 can be stably clamped between the positioning groove 5215 and the pressing plate 5211. Optionally, the conductive compression member 5222 can be a spring pin capable of conducting an electrical signal.

[0098] In some optional embodiments, in order to prevent the evaporation of the heated glucose solution in the tank 420, the circumferential side of the mounting table 510 and the constant temperature water bath 400 are sealed by a sealing member.

[0099] In some optional embodiments, as shown in Figure 14 and Figure 15 In order to prevent the evaporation of the heated glucose solution in the tank 420, the circumferential side of the plug-in area of the mounting table 510 and the sensor sealing module 520 are sealed by a sealing member 540a.

[0100] In some optional embodiments, as shown in Figure 11 In order to prevent the evaporation of the heated glucose solution in the tank 420, the adjacent sensor sealing modules 520 are sealed by a sealing member 540c.

[0101] In some optional embodiments, as shown in Figure 10As shown, the circuit board 5221 includes a signal collection board 5221a and a circuit processing board 5221b, the signal collection board 5221a is not welded with any components and is only used for inserting the conductive press members 5222, during assembly, the gold finger contact port of the circuit processing board 5221b is inserted into the female terminal connector in the signal collection board 5221a, so that the two are tightly combined. In order to ensure the airtightness between the circuit board 5221 and the mounting cavity 5214, a sealing member 540d is arranged around the mounting cavity 5214. Further, in order to prevent the circuit processing board 5221b from being affected by water vapor, a sealing member 540e is arranged at the joint of the signal collection board 5221a and the circuit processing board 5221b, and a sealing member 540f is arranged between the positioning groove 5215 and the pressing plate 5211.

[0102] In some optional embodiments, the sealing member 540a, the sealing member 540b, the sealing member 540c, and the sealing member 540d can be rubber sealing rings.

[0103] In some optional embodiments, as shown in Figure 1 The liquid storage tank 100, the flow member 200, the first driving mechanism 300, the constant-temperature water bath kettle 400, the sensor assembly 500, and the control processing unit 600 are arranged on the frame 10.

[0104] In some optional embodiments, as shown in Figure 1 The frame 10 is further provided with a waste liquid collection tank 20, which is used to store the discharged glucose solution in the storage tank 420 or the cleaned solution in the storage tank 420.

[0105] In some optional embodiments, as shown in Figure 1 The control processing unit 600 includes a PLC unit 610, an upper computer unit 620, and a data acquisition and display unit 630, the PLC unit 610 is responsible for motion control, temperature acquisition, signal acquisition, and other feedback and execution functions, the upper computer unit 620 is responsible for acquiring and processing data information of all sensors, and judging whether the sensors meet the requirements, and the data acquisition and display unit 630 mainly presents information content of the equipment screening test process.

[0106] In the description of the present specification, the description of the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0107] The above-described embodiments only express several implementation manners of the present application, the description is relatively specific and detailed, but cannot be understood as the limitation of the protection scope of the present application. It should be pointed out that, for ordinary skilled persons in the art, without departing from the concept of the present application, several modifications and improvements can be made, which all belong to the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the appended claims.

Claims

1. An electrochemical biosensor test with glucose solution perfusion assembly, characterized in that, The glucose solution perfusion assembly comprises: a liquid storage tank provided with a plurality of accommodating cavities for containing glucose solutions with different concentrations; a flow converging member having a liquid inlet end and a liquid outlet end, the liquid inlet end being in time communication with each of the accommodating cavities, and the liquid inlet end being higher than the liquid outlet end; a constant temperature water bath for receiving the glucose solution discharged from the liquid outlet end; a sensor assembly for fixing the electrochemical biosensor; wherein, when the glucose solution perfusion assembly is in a non-perfusion state, the sensor assembly is close to the constant temperature water bath until a reaction end of the electrochemical biosensor is immersed in the glucose solution, and the flow converging member is away from the constant temperature water bath; when the glucose solution perfusion assembly is in a perfusion state, the sensor assembly is away from the constant temperature water bath until the reaction end of the electrochemical biosensor is out of the glucose solution, and the flow converging member is close to the constant temperature water bath until the liquid outlet end is above a glucose solution receiving area of the constant temperature water bath.

2. The electrochemical biosensor test glucose solution perfusion assembly of claim 1, wherein, The glucose solution perfusion assembly comprises a first driving mechanism for driving the flow converging member to move towards or away from the constant temperature water bath, and the first driving mechanism comprises: a slide rail arranged beside the constant temperature water bath; a slide block slidingly arranged on the slide rail, and the flow converging member being arranged on the slide block; a driver in transmission connection with the slide block to push the slide block to move on the slide rail towards or away from the constant temperature water bath.

3. The electrochemical biosensor test glucose solution perfusion assembly of claim 1 or 2, wherein, The liquid storage tank comprises: a tank body provided with a first heating cavity for containing a heat transfer medium, and the accommodating cavities being arranged in the first heating cavity; a first heating assembly arranged in the first heating cavity for heating the heat transfer medium in the first heating cavity.

4. The electrochemical biosensor test glucose solution perfusion assembly of claim 3, wherein, The liquid storage tank comprises a first internal circulating water treatment assembly for circulating and guiding the heat transfer medium in the first heating cavity, and the first internal circulating water treatment assembly comprises: a first circulating water pump arranged in the first heating cavity or outside the first heating cavity, and having a first suction inlet and a first discharge outlet; a first shunt arranged in the first heating cavity, and having a plurality of first water inlets dispersed in the first heating cavity and a first water outlet, and the first water outlet being in communication with the first suction inlet; a second shunt arranged in the first heating cavity, and having a second water inlet and a plurality of second water outlets dispersed in the first heating cavity, and the second water inlet being in communication with the first discharge outlet.

5. The electrochemical biosensor test glucose solution perfusion assembly of claim 3, wherein, The first heating cavity is in communication with the flow converging member through an openable and closable pipeline assembly.

6. The electrochemical biosensor test glucose solution perfusion assembly of claim 1 or 2, wherein, The constant temperature water bath comprises: a pot body provided with a second heating cavity for containing a heat transfer medium; a tank pool arranged on the pot body, and the tank pool being at least partially immersed in the heat transfer medium in the second heating cavity; a second heating assembly arranged in the second heating cavity for heating the heat transfer medium in the second heating cavity.

7. The electrochemical biosensor test glucose solution perfusion assembly of claim 6, wherein, The constant temperature water bath comprises a second internal circulating water treatment assembly for circulating and guiding the heat transfer medium in the second heating cavity, and the second internal circulating water treatment assembly comprises: a second circulating water pump arranged in the second heating cavity or outside the second heating cavity, and having a second suction inlet and a second discharge outlet; A third shunt is arranged in the second heating cavity and has a plurality of third water inlets dispersed in the second heating cavity and a third water outlet, wherein the third water outlet is communicated with the second suction inlet; A fourth shunt is arranged in the second heating cavity and has a fourth water inlet and a plurality of fourth water outlets dispersed in the second heating cavity, wherein the fourth water inlet is communicated with the second discharge outlet.

8. The electrochemical biosensor test glucose solution perfusion assembly of claim 1, wherein, The sensor assembly comprises: The mounting table is provided with an insertion area in the middle, and the insertion area is provided with an insertion slot; The sensor sealing module is inserted into the insertion slot, and the sensor sealing module is provided with a fixing part for fixing the electrochemical biosensor at one end close to the constant-temperature water bath.

9. The electrochemical biosensor test glucose solution perfusion assembly of claim 8, wherein, The sensor assembly comprises a second driving mechanism in driving connection with the mounting table to drive the mounting table to approach or move away from the constant-temperature water bath.

10. A detection device, characterized in that The electrochemical biosensor test glucose solution perfusion assembly comprises the signal acquisition assembly, the control processing unit, and the sensor assembly. The signal acquisition assembly is used for collecting the electrical signal generated by the reaction between the electrochemical biosensor and the glucose solution. The control processing unit is used for controlling the operation state of the liquid storage tank and the constant-temperature water bath and processing the signal collected by the signal acquisition assembly.

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