Automatic antibody detection device

Abstract

The utility model discloses an automatic antibody detection device. The automatic antibody detection device comprises a multi-detection turntable, an automatic sample adding device and a plurality of detection chips, a plurality of detection stations are arranged on the top surface of the multi-detection turntable, the plurality of detection stations are annularly distributed, each detection station is correspondingly connected with one detection chip, the multi-detection turntable is connected with a driving mechanism, and the driving mechanism is connected with the automatic sample adding device. The driving mechanism drives the multiple detection rotary table to rotate around the axis of the multiple detection rotary table, the automatic sample adding device comprises a titration assembly and a control host, the control host is connected with the driving mechanism and the titration assembly, the titration assembly is used for dropwise adding a reagent to the detection chip, and the control host is used for adjusting the working states of the driving mechanism and the titration assembly. The antibody detection is realized by constructing the detection chip, and a plurality of detection stations can be integrated to realize multiple detection, so that the detection flux is improved. By arranging the automatic sample adding device, accurate injection of quantitative reagents can be realized without using a pipettor, so that the detection efficiency is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to detection technical field especially, relate to an antibody automatic detection device. BACKGROUND

[0002] Mycobacterium tuberculosis antibody detection as the key strategy of tuberculosis diagnosis and prevention and control, has the importance that can not be ignored. At present, mycobacterium tuberculosis antibody detection technology presents the diversification trend, covers enzyme-linked immunosorbent assay, colloidal gold immunochromatography technology, immunofluorescence detection and a variety of methods, but these methods have certain difference in the sensitivity and specificity of detection. Tuberculosis antibody detection technology has been widely applied in primary medical institutions, especially in remote areas and rural areas, provides important support for early detection and treatment of tuberculosis patients. Through mycobacterium tuberculosis antibody screening, latent infected persons and patients can be effectively identified, thereby reducing the risk of disease transmission, and striving for more critical treatment time window for patients.

[0003] At present, the main technical means of tuberculosis antibody screening includes enzyme-linked immunosorbent assay (ELISA) and colloidal gold immunochromatography. Enzyme-linked immunosorbent assay is a high-sensitivity immunological detection technology, which is widely used for detection of pathogen-specific antibodies. The technology is based on the principle of specific binding of antigen and antibody, and the color change of the substrate is detected by enzyme-labeled antibody, and then it is determined whether there is a specific antibody in the sample. ELISA technology has the advantages of high sensitivity, high specificity and quantitative analysis.

[0004] In the process of realizing the prior art, it is found that: enzyme-linked immunosorbent assay is limited for rapid diagnosis due to its complicated operation, long experimental period, and needs to be completed in a professional laboratory, and has high requirements for experimental equipment, which faces challenges in resource-limited areas. In contrast, colloidal gold immunochromatography technology as a rapid and simple detection means, through the specific binding of colloidal gold labeled antigen or antibody with antibody or antigen in the sample, and the result is displayed through the detection point on the test strip. The technology is simple and fast to operate, and does not need to rely on professional equipment, and is especially suitable for primary medical institutions and on-site rapid detection. However, the limitation of the structure of this technology is that it cannot realize automation and high-throughput detection, so its efficiency is relatively low when dealing with a large number of sample screening. UTILITY MODEL CONTENT

[0005] The main purpose of the utility model is to provide an antibody automatic detection device, so as to overcome the deficiencies in the prior art.

[0006] In order to realize the foregoing utility model purposes, the technical scheme adopted by the utility model includes:

[0007] The utility model provides a kind of antibody automatic detection device, including multiple detection turntable, automatic sample adding device and multiple detection chips, the multiple detection turntable top surface is equipped with multiple detection stations, and multiple detection stations are annularly distributed, every the detection station is connected with a detection chip, the multiple detection turntable is connected with driving mechanism, the driving mechanism is used to drive the multiple detection turntable rotates around its axis, the automatic sample adding device includes titration component, control host, the control host is connected with the driving mechanism, titration component, the titration component is used to drop reagent to detection chip, the control host is used to adjust the working condition of the driving mechanism and the titration component.

[0008] In some more specific embodiments, the titration component includes at least a peristaltic pump, a reagent delivery pipe, the peristaltic pump and the reagent delivery pipe are connected, the peristaltic pump is used to deliver reagent to the detection chip through the reagent delivery pipe.

[0009] Preferably, the titration component further includes a mechanical arm, the mechanical arm is connected with the control host, the mechanical arm includes forearm, middle arm and rear arm, the mechanical arm is fixed in titration position under the action of control host.

[0010] In some more specific embodiments, the detection chip includes a reaction window and a detection film, the reaction window is arranged on the surface of the detection film, the reaction window is used for adding sample and reagent and displaying detection results, and the detection film is provided with a plurality of non-overlapping detection points.

[0011] Preferably, the detection film is provided with a detection point and a control point, the detection point is used for antibody detection, and the control point is used for positive control analysis.

[0012] In some more specific embodiments, a first fixing mechanism is further included, the first fixing mechanism includes a first fixing member and a second fixing member, the first fixing member is arranged on the base portion of the detection chip, and the second fixing member is arranged on the multiple detection turntable, and the detection chip is detachably connected with the multiple detection turntable through the first fixing member and the second fixing member.

[0013] Preferably, the first fixing member is a first clamping groove, the second fixing member is a groove, and the detection chip is detachably connected with the multiple detection turntable through the first clamping groove and the groove.

[0014] In some more specific embodiments, the antibody automatic detection device further comprises a second fixing mechanism, the second fixing mechanism comprises a third fixing member and a fourth fixing member, the third fixing member is arranged on the base portion of the multiplex detection turntable, the fourth fixing member is arranged on the automatic sample adding device, and the multiplex detection turntable is detachably connected with the automatic sample adding device through the third fixing member and the fourth fixing member.

[0015] Preferably, the third fixing member is a second clamping groove, and the second fixing member is a convex groove.

[0016] In some more specific embodiments, the antibody automatic detection device comprises x automatic sample adding devices, x is greater than or equal to 2, the x automatic sample adding devices comprise a control host and x titration assemblies, each titration assembly stores a corresponding reagent, and the control host is connected with the titration assemblies and is used to adjust the working state of each titration assembly.

[0017] In some more specific embodiments, the antibody automatic detection device further comprises an assembly cavity, the control host, the peristaltic pump and the driving mechanism are arranged inside the assembly cavity, and the reagent conveying pipeline, the reagent storage and the mechanical arm are arranged outside the assembly cavity.

[0018] Preferably, the antibody automatic detection device further comprises a power supply, and the power supply is electrically connected with the control host.

[0019] More preferably, the power supply is a dry battery.

[0020] Compared with the prior art, the antibody automatic detection device has at least the following advantages:

[0021] First, the antibody automatic detection device provided by the present application realizes flexible detection of multiple samples through the multiplex detection turntable, and significantly improves the detection throughput.

[0022] Second, the antibody automatic detection device provided by the present application realizes accurate injection of quantitative reagents without using a pipette through the automatic sample adding device, and realizes semi-automatic operation of the detection process through the cooperative work of the peristaltic pump and the multiplex detection turntable, thereby effectively improving the detection efficiency.

[0023] Third, the control host of the antibody automatic detection device provided by the present application is connected with the dry battery, which ensures the convenience and speed of operation and meets the demand for instant detection. The device does not need to rely on professional equipment and is suitable for primary medical institutions and on-site rapid detection, and has a wide range of application scenarios. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 is a structural schematic view of an antibody automatic detection device provided by an embodiment of the present application;

[0025] Figure 2 is a structural schematic diagram of a detection chip provided by an embodiment of the present application;

[0026] Figure 3 is a front view of a multiple detection turntable provided by an embodiment of the present application;

[0027] Figure 4 is a rear view of a multiple detection turntable provided by an embodiment of the present application;

[0028] Figure 5 is a cross-sectional top view of an antibody automatic detection device provided by an embodiment of the present application.

[0029] Explanation of reference signs:

[0030] 11, first clamping groove; 12, reaction window; 13, detection film; 131, detection point; 132, control point; 21, groove; 22, second clamping groove; 31, single-chip microcomputer chip; 32, peristaltic pump; 33, stepping motor; 341, reagent conveying pipeline; 342, reagent bin; 343, mechanical arm; 35, dry battery; 36, convex groove. DETAILED DESCRIPTION

[0031] In view of the deficiencies in the prior art, the present application has been developed through long-term research and a large number of practices. The technical solution, implementation process and principles thereof will be further explained and described as follows.

[0032] Please refer to Figure 1 The present embodiment provides an automatic detection device for tuberculosis antibodies, comprising a multiple detection turntable, an automatic sample adding device and a plurality of detection chips.

[0033] As shown in Figure 2 , the detection chip comprises a first clamping groove 11, a reaction window 12 and a detection film 13. The first clamping groove 11 is arranged at the bottom of the detection chip. The detection film 13 includes, but is not limited to, a nitrocellulose film. The reaction window 12 is arranged on the surface of the detection film 13, and is used for adding samples and reagents, and for displaying detection results. The detection film 13 is configured with a detection point 131 and a control point 132. The detection point 131 is a tuberculosis antibody detection antigen, and is used for tuberculosis mycobacterium antibody detection. The control point 132 is a positive control point, and is used for positive control analysis.

[0034] As shown in Figure 3As shown, the top surface of the multiple detection turntable is provided with eight detection stations, and the eight detection stations are annularly distributed, each of the detection stations being correspondingly connected with a detection chip. The detection station of the multiple detection turntable is provided with a groove 21. The first clamping groove 11 is detachably connected with the groove 21 to fix the detection chip on the multiple detection turntable.

[0035] As shown, Figure 4 The bottom surface of the multiple detection turntable is provided with a second clamping groove 22. The top surface of the stepping motor 33 is provided with a convex groove 36, and the second clamping groove 22 is detachably connected with the convex groove 36 to fix the multiple detection turntable on the stepping motor 33. The stepping motor 33 is used to drive the multiple detection turntable to rotate around the axis thereof. It should be noted that the top surface of the stepping motor 33 can also be provided with a groove, and the second clamping groove 22 is detachably connected with the groove.

[0036] As shown, Figure 5 The automatic sample adding device comprises a single-chip microcomputer 31, a titration assembly, and a dry battery 35. The titration assembly comprises a peristaltic pump 32, a reagent conveying pipeline 341, a reagent bin 342, and a mechanical arm 343.

[0037] Specifically, the single-chip microcomputer 31 is used to program and control the peristaltic pump 32 and the stepping motor 33. The peristaltic pump 32 is used to control the liquid system to realize automatic adding of reagents. Generally, the peristaltic pump is composed of three main components: a driving device, a pump head, and a hose. The pump can realize closed conveying of fluid in the pump pipe, support quick replacement of the pump pipe, allow reverse flow of fluid, and dry operation. Between the two rotating rollers, the peristaltic pump forms a "pillow"-shaped fluid area, the size of which is jointly affected by the inner diameter of the pump pipe and the geometry of the rotor. According to its operating characteristics and application requirements, the peristaltic pump can be further divided into speed-regulating, flow, dispensing, and customized peristaltic pumps. In this embodiment, the selection of the peristaltic pump should be based on the actual operation requirements to determine the most suitable model. In this embodiment, the mechanical arm 343 is composed of a front arm, a middle arm, and a rear arm. Under the control of the single-chip microcomputer 31, the mechanical arm can stably position the reagent conveying pipeline 341 at the titration position. In this way, the angle, height, and position of the mechanical arm can be flexibly adjusted, thereby realizing high-precision control of the positioning of the reagent conveying pipeline.

[0038] The stepper motor 33 is also used to drive the multiple detection turntable to cooperate with the titration assembly to complete the automatic sample adding of different samples. Preferably, the single-chip microcomputer chip 31, the peristaltic pump 32, the stepper motor 33 and the dry battery 35 are located inside the assembly cavity. The reagent delivery pipeline 341, the reagent bin 342 and the mechanical arm 343 are located outside the assembly cavity. Based on this, the device can be operated only by dry battery power, significantly reducing the dependence on large supporting equipment, and its structure is simple, light in weight, small in size, easy to carry and easy to operate.

[0039] In some schemes, the antibody automatic detection device comprises a first automatic sample adding device and a second automatic sample adding device, and the two automatic sample adding devices comprise a control host, a first titration assembly and a second titration assembly, each titration assembly stores corresponding reagents, and is connected with the control host, and the control host is used to adjust the working state of each titration assembly.

[0040] With the peripheral blood of tuberculosis patients and healthy examination personnel as a demonstration example, the operation method of the device is as follows:

[0041] (1) First, assemble the detection chip on the multiple detection turntable. According to the number of samples, it can be flexibly switched between 1-8 samples. Then, assemble the multiple detection turntable on the automatic sample adding device.

[0042] (2) Add 1 drop of serum sample (about 50 microliters) on the detection chip.

[0043] (3) Start the first automatic sample adding device, use the power of the peristaltic pump 32 to suck about 100 microliters of colloidal gold developing agent from the reagent bin A, and accurately drop it on the surface of the detection chip through the mechanical arm 343.

[0044] (4) After the colloidal gold reaction solution is absorbed, start the second automatic sample adding device. Under the driving action of the peristaltic pump 32, about 100 microliters of cleaning solution is sucked from the reagent bin B, and is accurately dropped on the surface of the detection chip 1 through the mechanical arm 343.

[0045] (5) After the experimental operation, wait for 30 seconds, and according to the color development reaction of the positive quality control point and the tuberculosis antibody detection antigen, the tuberculosis patients and the healthy examination personnel can be distinguished. Specifically, the positive quality control point and the tuberculosis antibody detection antigen in the tuberculosis patient sample will all show color development reaction; relatively, only the positive quality control point of the healthy examination personnel sample shows color development, while the tuberculosis antibody detection antigen point does not show color development reaction. Through this color development difference, the positive and negative results of the sample can be accurately determined.

[0046] In summary, the utility model integrates the tuberculosis antibody rapid automation detection and analysis function. The device only needs dry battery as energy source to realize operation, compared with large equipment connected with commercial power, the dependence on large supporting equipment is reduced obviously. Compared with traditional enzyme linked immunosorbent assay, the device is simple to operate, and compared with manual colloidal gold test strip method, has the high throughput detection capacity, improves the detection efficiency. In addition, the production cost of the equipment is lower, which is beneficial to the popularization and application in large scale. The utility model realizes the detection of mycobacterium tuberculosis antibody by constructing the detection chip, and can integrate a variety of antibody detection stations according to experimental requirements to achieve multiplex detection. Through the designed multiplex detection turntable, flexible detection of 1 to 8 samples is realized, so that the detection throughput is improved. At the same time, through the setting of the automatic sample adding device, the quantitative reagent can be accurately injected into the detection chip without using a pipette, and the semi-automatic operation of the detection process is realized by the cooperative work of the peristaltic pump and the multiplex detection turntable.

[0047] The utility model realizes the rapid, convenient, automatic mycobacterium tuberculosis antibody detection from human peripheral blood sample. Compared with traditional enzyme linked immunosorbent assay, the utility model does not need to rely on large supporting equipment, and simplifies the operation process. Compared with manual colloidal gold test strip method, the utility model can realize high throughput detection, and ensures the rapidity of detection. In addition, the device has the advantages of low cost, simple structure, light weight, small size, is convenient to carry, has significant cost benefit, is not only suitable for application in field environment, but also suitable for use in hospital and other professional environments, has simple and fast operation, does not depend on power supply, professional instrument or technical personnel, meets the demand of instant detection, and has huge market potential.

[0048] It should be understood that the above embodiments are only for illustrating the technical concept and characteristics of the utility model, the purpose is to enable those skilled in the art to understand the content of the utility model and implement it, and cannot limit the protection scope of the utility model. Any equivalent change or modification according to the spirit and essence of the utility model should be covered in the protection scope of the utility model.

Claims

1. An antibody automatic detection apparatus characterized by comprising: The multiple detection carousel, the automatic sample adding device and the multiple detection chips are provided, the top surface of the multiple detection carousel is provided with multiple detection stations which are arranged in a ring shape, each detection station is connected with a detection chip, the multiple detection carousel is connected with a driving mechanism, the driving mechanism is used for driving the multiple detection carousel to rotate around its axis, the automatic sample adding device comprises a titration assembly and a control host, the control host is connected with the driving mechanism and the titration assembly, the titration assembly is used for adding reagents to the detection chip, and the control host is used for adjusting the working states of the driving mechanism and the titration assembly.

2. The antibody automatic detection apparatus according to claim 1, characterized by The titration assembly at least comprises a peristaltic pump and a reagent conveying pipeline, the peristaltic pump is connected with the reagent conveying pipeline, and the peristaltic pump is used for conveying reagents to the detection chip through the reagent conveying pipeline.

3. The antibody auto-detection device according to claim 2, characterized by The titration assembly further comprises a mechanical arm, the mechanical arm is connected with the control host, the mechanical arm comprises a front arm, a middle arm and a rear arm, and the mechanical arm is fixed at a titration position under the action of the control host.

4. The antibody automatic detection apparatus according to claim 1, characterized by The detection chip comprises a reaction window and a detection film, the reaction window is arranged on the surface of the detection film, the reaction window is used for adding samples and reagents and displaying detection results, and the detection film is provided with multiple non-overlapping detection points.

5. The antibody automatic detection apparatus according to claim 4, wherein The detection film is provided with a detection point and a control point, the detection point is used for antibody detection, and the control point is used for positive control analysis.

6. The antibody auto-detection device according to claim 1, wherein Further, a first fixing mechanism is provided, the first fixing mechanism comprises a first fixing part and a second fixing part, the first fixing part is arranged on the base part of the detection chip, the second fixing part is arranged on the multiple detection carousel, and the detection chip is detachably connected with the multiple detection carousel through the first fixing part and the second fixing part.

7. The antibody auto-detection device according to claim 6, wherein The first fixing part is a first clamping groove, the second fixing part is a groove, and the detection chip is detachably connected with the multiple detection carousel through the first clamping groove and the groove.

8. The antibody auto-detection device according to claim 6, wherein Further, a second fixing mechanism is provided, the second fixing mechanism comprises a third fixing part and a fourth fixing part, the third fixing part is arranged on the base part of the multiple detection carousel, the fourth fixing part is arranged on the automatic sample adding device, and the multiple detection carousel is detachably connected with the automatic sample adding device through the third fixing part and the fourth fixing part.

9. The antibody automatic detection apparatus according to claim 8, wherein The third fixing part is a second clamping groove, and the second fixing part is a convex groove.

10. The antibody auto-detection apparatus according to claim 1, characterized by The x automatic sample adding devices comprise a control host and x titration assemblies, each titration assembly stores corresponding reagents and is connected with the control host, and the control host is used for adjusting the working states of each titration assembly.

11. The antibody auto-detection device according to claim 2, characterized by Further, an assembly cavity is provided, the control host, the peristaltic pump and the driving mechanism are arranged in the assembly cavity, and the reagent conveying pipeline, the reagent storage and the mechanical arm are arranged outside the assembly cavity.

12. The antibody auto-detection apparatus according to claim 1, wherein Further, a power supply is provided, the power supply is electrically connected with the control host.

13. The antibody auto-detection device according to claim 12, wherein The power supply is a dry battery.

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