Protein extraction scraper device

Abstract

The utility model relates to the technical field of protein extraction, and discloses a protein extraction scraper device which comprises a base, a scraper assembly is arranged at the top of the base, the scraper assembly comprises fixing plates, a sliding groove, a rotating shaft, a first connecting rod, a second connecting rod, a connecting plate, a scraper mechanism, a guide rail and a sliding block, and the fixing plates are arranged on the two sides of the top of the base; sliding grooves are formed in the fixing plates, a rotating shaft is arranged between the two fixing plates, and first connecting rods are arranged at the two ends of the rotating shaft. According to the protein extraction scraper device, by arranging the scraper assembly, manual cell scraping can be replaced, traditional cell scraping is replaced, the labor intensity of workers is reduced, a driving motor is started to be matched with a rotating shaft, a first connecting rod, a second connecting rod and a connecting plate, and a scraper mechanism can be driven to move downwards; the stepping motor is started to drive the scraper blade to rotate, and bottom cells of the culture dish are scraped.

Description

TECHNICAL FIELD

[0001] The utility model relates to protein extraction technical field, concretely is a kind of protein extraction scraper device. BACKGROUND

[0002] Protein extraction experiment is aimed at separating and obtaining protein from biological samples such as cells or tissues, so as to carry out subsequent analysis and research, and the protein extraction experiment will be different due to the difference of the object extracted and the method used, and the common cell extraction protein extraction experiment steps include; Cell flushing, adding lysing solution, scraping off cells, centrifugal collection protein and other steps.

[0003] There are still the following problems in actual use process:

[0004] After adding lysing solution in protein extraction experiment, personnel uses cell scraper to scrape back and forth the bottom of culture dish after placing for a period of time, until the bottom of culture dish is clean, personnel handholds cell scraper to operate in prior art, not only increase the labor intensity of experimental personnel, and manual operation cannot guarantee the standardization of each experiment. UTILITY MODEL CONTENT

[0005] In view of the deficiencies of the prior art, the utility model provides a kind of protein extraction scraper device, with the advantages of being able to automatically scrape the bottom of culture dish, replace traditional cell scraper, reduce the labor intensity of personnel, ensure the standardization of experiment, solve the problems raised in background art.

[0006] The utility model provides the following technical scheme: a kind of protein extraction scraper device, including base, the top of the base is provided with scraper assembly;

[0007] The scraper assembly includes fixed plate, sliding slot, shaft, connecting rod one, connecting rod two, connecting plate, scraper mechanism, guide rail, sliding block, the fixed plate is set at the top of base both sides, the fixed plate is internally provided with sliding slot, the shaft is provided between the two fixed plate, the shaft is provided with connecting rod one at both ends, the connecting rod one is provided with connecting rod two at one end, the connecting rod two is provided with connecting plate at one end, the connecting plate is provided with scraper mechanism at one end, the inner side of the fixed plate is provided with guide rail, the surface of the guide rail is provided with sliding block.

[0008] Preferably, the top of the base is provided with positioning assembly, the top of the base is installed with culture dish by positioning assembly, the positioning assembly includes positioning plate, groove, sliding rod, arc clamping plate, movable plate, spring, the top of the base is fixedly connected with positioning plate, the positioning plate is X-shaped and four ends are provided with grooves, respectively, the inside of the groove is slidably connected with sliding rod, one end of the sliding rod is fixedly connected with arc clamping plate, the other end of the sliding rod is fixedly connected with movable plate, the surface of the sliding rod is sleeved with spring.

[0009] Preferably, the fixed plate is fixedly connected with the top of the base, the rotating shaft is rotatably connected with the fixed plate, one end of the first connecting rod is fixedly connected with the rotating shaft, one end of the second connecting rod is rotatably connected with the first connecting rod, the connecting plate is movably connected with one end of the second connecting rod, the scraper mechanism is fixedly connected with one end of the connecting plate, the guide rail is fixedly connected with the inner side of the fixed plate, the sliding block is slidably connected with the surface of the guide rail, and one side of the sliding block is fixedly connected with the connecting plate.

[0010] Preferably, the scraper mechanism comprises a mounting plate, a stepping motor and a scraper blade, one end of the connecting plate is fixedly connected with the mounting plate, the top of the mounting plate is fixedly provided with the stepping motor, and the output shaft of the stepping motor is fixedly connected with the scraper blade.

[0011] Preferably, one side of the fixed plate is fixedly connected with an L-shaped plate, one side of the L-shaped plate is fixedly provided with a driving motor, and the output shaft of the driving motor is fixedly connected with one end of the rotating shaft.

[0012] Preferably, one end of the spring is fixedly connected with the movable plate, and the other end of the spring is fixedly connected with the inner wall of the groove.

[0013] Preferably, the scraper blade is located directly above the culture dish.

[0014] Compared with the prior art, the protein extraction scraper device has the following beneficial effects:

[0015] 1. The protein extraction scraper device, by setting the scraper assembly, can replace manual scraping of cells, replace traditional cell scraping, reduce labor intensity, and by starting the driving motor, cooperating with the rotating shaft, the first connecting rod, the second connecting rod and the connecting plate, the scraper mechanism can be driven to move downwards, when the scraper blade moves to the inside of the culture dish and is attached to the bottom of the culture dish, the stepping motor is started to drive the scraper blade to rotate, and the cells on the bottom of the culture dish are scraped.

[0016] 2. The protein extraction scraper device, by setting the positioning assembly, the culture dish can be clamped and positioned, the culture dish is pushed into the four arc-shaped clamping plates, the culture dish is clamped and fixed through the reverse action force of the spring, and the culture dish is convenient to fix and disassemble. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 The device overall structure schematic view of the protein extraction scraper device provided by the utility model is shown in the figure;

[0018] Figure 2 The scraper assembly structure schematic view of the protein extraction scraper device provided by the utility model is shown in the figure;

[0019] Figure 3The utility model provides a kind of scraper mechanism structure schematic diagram of protein extraction scraper device.

[0020] Figure 4 The utility model provides a kind of positioning assembly structure schematic diagram of protein extraction scraper device.

[0021] Figure 5 The utility model provides a kind of scraper mechanism structure schematic diagram of protein extraction scraper device. Figure 4 The utility model provides a kind of scraper mechanism structure schematic diagram of protein extraction scraper device.

[0022] In the figure: 1, base; 2, culture dish; 3, scraper assembly; 31, fixed plate; 32, sliding groove; 33, shaft; 34, connecting rod one; 35, connecting rod two; 36, connecting plate; 37, scraper mechanism; 371, mounting plate; 372, stepper motor; 373, scraper blade; 38, guide rail; 39, sliding block; 4, L-shaped plate; 5, drive motor; 6, positioning assembly; 61, positioning plate; 62, groove; 63, sliding rod; 64, arc clamping plate; 65, movable plate; 66, spring. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0024] Please refer to Figures 1 to 3 A kind of protein extraction scraper device, including base 1, the top of base 1 is provided with scraper assembly 3, scraper assembly 3 includes fixed plate 31, sliding groove 32, shaft 33, connecting rod one 34, connecting rod two 35, connecting plate 36, scraper mechanism 37, guide rail 38, sliding block 39, fixed plate 31 is set at the top of base 1 two sides, sliding groove 32 is set in fixed plate 31 inside, two fixed plates 31 are provided with shaft 33, the two ends of shaft 33 are provided with connecting rod one 34, one end of connecting rod one 34 is provided with connecting rod two 35, one end of connecting rod two 35 is provided with connecting plate 36, one end of connecting plate 36 is provided with scraper mechanism 37, the inside of fixed plate 31 is provided with guide rail 38, the surface of guide rail 38 is provided with sliding block 39;

[0025] By being provided with scraper assembly 3, cell can be scraped instead of manually, replace traditional cell scraping, reduce personnel labor intensity, by starting drive motor 5, cooperate shaft 33, connecting rod one 34, connecting rod two 35, connecting plate 36, can drive scraper mechanism 37 to move downwards, and scrape the cell at the bottom of culture dish 2.

[0026] Please refer to Figures 1 to 3 The fixed plate 31 is fixedly connected to the top of the base 1, the rotating shaft 33 is rotatably connected to the fixed plate 31, the first connecting rod 34 is fixedly connected to one end of the rotating shaft 33, the second connecting rod 35 is rotatably connected to one end of the first connecting rod 34, the connecting plate 36 is movably connected to one end of the second connecting rod 35, the scraper mechanism 37 is fixedly connected to one end of the connecting plate 36, the guide rail 38 is fixedly connected to the inner side of the fixed plate 31, the sliding block 39 is slidably connected to the surface of the guide rail 38, and one side of the sliding block 39 is fixedly connected to the connecting plate 36. One side of the fixed plate 31 is fixedly connected with an L-shaped plate 4, one side of the L-shaped plate 4 is fixedly installed with a driving motor 5, and the output shaft of the driving motor 5 is fixedly connected to one end of the rotating shaft 33.

[0027] By starting the driving motor 5, the rotating shaft 33 is driven to rotate, and the first connecting rod 34 is driven to rotate, cooperating with the second connecting rod 35, so that the connecting plate 36 is pushed to slide in the sliding groove 32, and the connecting plate 36 is driven to move downward, and the scraper mechanism 37 is driven to move downward.

[0028] Please refer to Figure 1 and Figure 3 The scraper mechanism 37 comprises a mounting plate 371, a stepping motor 372 and a scraper blade 373. One end of the connecting plate 36 is fixedly connected with the mounting plate 371, the top of the mounting plate 371 is fixedly installed with the stepping motor 372, the output shaft of the stepping motor 372 is fixedly connected with the scraper blade 373, and the scraper blade 373 is located directly above the culture dish 2.

[0029] When the scraper blade 373 moves into the culture dish 2 and is attached to the bottom of the culture dish 2, the stepping motor 372 is started to drive the scraper blade 373 to rotate, so as to scrape the cells on the bottom of the culture dish 2, instead of manual operation by personnel.

[0030] Please refer to Figures 1 to 5 The top of the base 1 is provided with a positioning assembly 6, and the culture dish 2 is installed on the top of the base 1 through the positioning assembly 6. The positioning assembly 6 comprises a positioning plate 61, a groove 62, a sliding rod 63, an arc-shaped clamping plate 64, a movable plate 65 and a spring 66. The top of the base 1 is fixedly connected with the positioning plate 61, the positioning plate 61 is X-shaped and has four ends respectively provided with the grooves 62, the sliding rod 63 is slidably connected in the grooves 62, one end of the sliding rod 63 is fixedly connected with the arc-shaped clamping plate 64, the other end of the sliding rod 63 is fixedly connected with the movable plate 65, and the spring 66 is sleeved on the surface of the sliding rod 63, one end of the spring 66 is fixedly connected with the movable plate 65, and the other end of the spring 66 is fixedly connected with the inner wall of the groove 62.

[0031] Through the positioning assembly 6, the culture dish 2 can be clamped and positioned, and the culture dish 2 is pushed into the four arc-shaped clamping plates 64, and the culture dish 2 is clamped and fixed through the reverse force of the spring 66, so that personnel can conveniently fix and disassemble the culture dish 2.

[0032] In the utility model, the working principle of the device is as follows:

[0033] In the protein extraction experiment, after the lysis solution is added into the culture dish 2, the experimental personnel hold the culture dish 2 and make the culture dish 2 adhere to the two arc-shaped clamping plates 64 in front, then push the culture dish 2 to drive the two arc-shaped clamping plates 64 in front to move, so that the two arc-shaped clamping plates 64 move forward, the sliding rod 63 and the movable plate 65 are driven to move, and the spring 66 is deformed under stress, then when the distance between the arc-shaped clamping plates 64 on the front and back sides increases, the culture dish 2 can be placed on the positioning plate 61, and through the reverse force of the spring 66, the arc-shaped clamping plate 64 drives the culture dish 2 to be clamped and fixed;

[0034] After the culture dish 2 is fixed, the culture dish 2 is static for a period of time, then the driving motor 5 is started to drive the rotating shaft 33 to rotate, and the connecting plate 36 is driven to slide in the sliding groove 32, so that the connecting plate 36 moves downward, and the scraper mechanism 37 moves downward, when the scraper blade 373 moves into the culture dish 2 and adheres to the bottom of the culture dish 2, the stepping motor 372 is started to drive the scraper blade 373 to rotate, and the cells on the bottom of the culture dish 2 are scraped, so that manual operation is replaced, after the bottom of the culture dish 2 is clean, the driving motor 5 is driven to drive the rotating shaft 33 to rotate reversely, so that the scraper mechanism 37 rises, the scraper blade 373 is moved out of the culture dish 2, and subsequent experimental steps are carried out.

[0035] It should be noted that, in the present document, relational terms such as first and second and the like can be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises", "comprising", or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can include other elements not expressly listed or inherent to such process, method, article, or apparatus.

[0036] Although the embodiments of the utility model have been shown and described, it should be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to the embodiments without departing from the principles and spirits of the utility model, and the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A protein extraction scraper device comprising a base (1), characterized in that, The top of the base (1) is provided with a scraper assembly (3); The scraper assembly (3) comprises a fixed plate (31), a sliding groove (32), a rotating shaft (33), a connecting rod one (34), a connecting rod two (35), a connecting plate (36), a scraper mechanism (37), a guide rail (38), and a sliding block (39). The fixed plate (31) is arranged on both sides of the top of the base (1), and the sliding groove (32) is arranged in the fixed plate (31). The rotating shaft (33) is arranged between the two fixed plates (31), and the connecting rod one (34) is arranged at both ends of the rotating shaft (33). The connecting rod two (35) is arranged at one end of the connecting rod one (34), and the connecting plate (36) is arranged at one end of the connecting rod two (35). The scraper mechanism (37) is arranged at one end of the connecting plate (36), and the guide rail (38) is arranged on the inner side of the fixed plate (31). The surface of the guide rail (38) is provided with the sliding block (39).

2. A protein extraction scraper device according to claim 1, wherein: The top of the base (1) is provided with a positioning assembly (6), and the top of the base (1) is provided with a culture dish (2) through the positioning assembly (6). The positioning assembly (6) comprises a positioning plate (61), a groove (62), a sliding rod (63), an arc-shaped clamping plate (64), a movable plate (65), and a spring (66). The top of the base (1) is fixedly connected with the positioning plate (61), the positioning plate (61) is X-shaped and has four ends respectively provided with the groove (62), the sliding rod (63) is slidably connected in the groove (62), one end of the sliding rod (63) is fixedly connected with the arc-shaped clamping plate (64), the other end of the sliding rod (63) is fixedly connected with the movable plate (65), and the surface of the sliding rod (63) is sleeved with the spring (66).

3. A protein extraction scraper device according to claim 1, wherein: The fixed plate (31) is fixedly connected with the top of the base (1), the rotating shaft (33) is rotatably connected with the fixed plate (31), one end of the connecting rod one (34) is fixedly connected with the rotating shaft (33), one end of the connecting rod two (35) is rotatably connected with the connecting rod one (34), one end of the connecting rod two (35) is movably connected with the connecting plate (36), one end of the connecting plate (36) is fixedly connected with the scraper mechanism (37), the inner side of the fixed plate (31) is fixedly connected with the guide rail (38), and the surface of the guide rail (38) is slidably connected with the sliding block (39), and one side of the sliding block (39) is fixedly connected with the connecting plate (36).

4. The protein extraction scraper device of claim 1, wherein: The scraper mechanism (37) comprises a mounting plate (371), a stepping motor (372), and a scraper blade (373). One end of the connecting plate (36) is fixedly connected with the mounting plate (371), the top of the mounting plate (371) is fixedly connected with the stepping motor (372), and the output shaft of the stepping motor (372) is fixedly connected with the scraper blade (373).

5. The protein extraction scraper device of claim 1, wherein: One side of the fixed plate (31) is fixedly connected with an L-shaped plate (4), one side of the L-shaped plate (4) is fixedly connected with a driving motor (5), and the output shaft of the driving motor (5) is fixedly connected with one end of the rotating shaft (33).

6. A protein extraction scraper device according to claim 2, wherein: One end of the spring (66) is fixedly connected with the movable plate (65), and the other end of the spring (66) is fixedly connected with the inner wall of the groove (62).

7. A protein extraction scraper device according to claim 4, wherein: The doctor blade blade (373) is located directly above the petri dish (2).

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