Column passing device and experimental equipment

Abstract

The utility model discloses a column passing device and experimental equipment. The column passing device comprises a butt joint module, a first pressing plate module and a first driving module, the butt joint module is used for communicating a liquid outlet of the chromatographic column with the liquid receiving device; the first pressing plate module and the butt joint module are oppositely arranged, the first pressing plate module is provided with a first liquid inlet channel, one end of the first liquid inlet channel is used for being communicated with a liquid inlet of the chromatographic column, and the other end of the first liquid inlet channel is used for being communicated with a liquid supply device; the first driving module is connected with the first pressing plate module, and the first driving module is used for driving the first pressing plate module to be close to or away from the butt joint module. The column passing device can solve the problems of low sample separation and purification efficiency and high cost of a chromatographic experiment in the prior art.

Description

TECHNICAL FIELD

[0001] The utility model relates to experimental equipment technical field, concretely relates to a pass through column device and experimental equipment. BACKGROUND

[0002] Chromatography is a technology that separates different substances by their different distribution coefficients between stationary phase and mobile phase. In physical and chemical experiments, chromatography is widely used in component separation, purification, identification and quality control of multi-component samples. However, in the chromatography experiment of the prior art, a large amount of manual intervention is required during the separation and purification process of sample components, resulting in high labor cost and low efficiency. UTILITY MODEL CONTENT

[0003] The utility model aims at providing a pass through column device and experimental equipment, solves the problems of low separation and purification efficiency and high cost of sample components in the chromatography experiment of the prior art.

[0004] To achieve the purpose of the utility model, the utility model provides the following technical scheme:

[0005] In a first aspect, the utility model provides a pass through column device, which comprises a docking module, a first pressing plate module and a first driving module; the docking module is used for connecting a liquid outlet of a chromatography column and a liquid receiving device; the first pressing plate module is arranged opposite to the docking module, and the first pressing plate module has a first liquid inlet channel, one end of the first liquid inlet channel is used for connecting a liquid inlet of the chromatography column, and the other end of the first liquid inlet channel is used for connecting a liquid supply device; the first driving module is connected with the first pressing plate module, and the first driving module is used for driving the first pressing plate module to move close to or away from the docking module.

[0006] In an embodiment, the pass through column device further comprises a first plug pulling module, and the first plug pulling module is used for pulling down a lower plug located at the liquid outlet of the chromatography column.

[0007] In an embodiment, the first plug pulling module comprises a fixed component and a movable component, the fixed component is slidably connected with the movable component, a clamping groove is formed in the fixed component, and the movable component is used for abutting against the lower plug of the chromatography column and sliding relative to the fixed component, so that the lower plug of the chromatography column is clamped into the clamping groove.

[0008] In an embodiment, the fixed component comprises a mounting seat and a clamping plate, the movable component is arranged on the mounting seat, and the movable component is slidably connected with the mounting seat; the clamping plate is arranged opposite to and spaced apart from the mounting seat, the movable component is arranged between the mounting seat and the clamping plate, and the clamping groove penetrates through the clamping plate along the thickness direction of the clamping plate.

[0009] In one embodiment, the movable assembly comprises a support plate, a spring plunger and a first guide post, the support plate is arranged between the mounting seat and the clamping plate; the spring plunger comprises a fixed pin, a movable pin and a first elastic member, the fixed pin is arranged in the mounting seat and is fixedly connected with the mounting seat, the movable pin is connected with the support plate, and the first elastic member is elastically deformed along the thickness direction of the support plate with two opposite ends of the first elastic member being connected with the fixed pin and the movable pin respectively; the first guide post is arranged in the mounting seat and is slidingly connected with the mounting seat, the first guide post is arranged in parallel with the spring plunger, one end of the first guide post is connected with the support plate, and the other end of the first guide post is a free end.

[0010] In one embodiment, the movable assembly further comprises a liquid guide plate, the liquid guide plate is arranged between the mounting seat and the support plate, and the liquid guide plate is connected with the support plate; the liquid guide plate comprises a flow guide surface, the flow guide surface is arranged around the edge of the support plate and protrudes from the support plate, the flow guide surface and the support plate have an included angle, and the flow guide surface extends away from the mounting seat from the support plate.

[0011] In one embodiment, the first plug module further comprises a bottom plate and a vertical plate, the vertical plate is connected with the bottom plate, the mounting seat is located between the bottom plate and the clamping plate, the mounting seat is arranged in opposite spaced relation with the bottom plate, and the clamping plate is connected with the vertical plate.

[0012] In one embodiment, the docking module comprises a support seat, an inlet joint group and an outlet joint group, the inlet joint group is arranged on the support seat, the inlet joint group comprises N first joints, the first joints are used for connecting the liquid outlet of the chromatographic column, and N is an integer greater than or equal to 1; the outlet joint group is arranged on the support seat, the outlet joint group comprises N second joints, the second joints and the first joints are in one-to-one correspondence and are connected, and the second joints are also used for connecting the liquid connection device.

[0013] In one embodiment, the first pressure plate module comprises a first pressure plate and N first liquid guide members, the first liquid guide members are arranged in the first pressure plate, the first liquid guide members enclose the first liquid inlet channel, the first liquid guide members comprise a first plug and a second plug, the first plug and the second plug are respectively located on two opposite sides of the first pressure plate, the first plug is used for connecting the liquid inlet of the chromatographic column, the second plug is used for connecting the liquid supply device, and N is an integer greater than or equal to 1.

[0014] In one embodiment, the first pressing plate module further comprises a first guide block and a first buffer assembly. The first guide block is arranged on a side of the first pressing plate opposite to the second plug. N first guide holes are formed in the first guide block. The first guide holes are arranged opposite to the first plug. The first buffer assembly is arranged between the first guide block and the first pressing plate. The first buffer assembly is used to move the first guide block relative to the first pressing plate, so that the first plug passes through the first guide hole. The first buffer assembly comprises a second elastic member and a second guide column. One of the first pressing plate and the first guide block is fixedly connected to one end of the second guide column. The other is slidingly connected to the other end of the second guide column. The second elastic member surrounds the second guide column. One end of the second elastic member abuts against the first pressing plate. The other end of the second elastic member abuts against the first guide block.

[0015] In one embodiment, the over column device further comprises a second pressing plate module. The second pressing plate module is arranged opposite to the first pressing plate module. The other end of the first liquid inlet channel is used to communicate with the liquid outlet of the sample column. The second pressing plate module has a second liquid inlet channel. One end of the second liquid inlet channel is used to communicate with the liquid inlet of the sample column. The other end of the second liquid inlet channel is used to communicate with the liquid supply device.

[0016] In one embodiment, the second pressing plate module comprises a second pressing plate and N second liquid guides. The second liquid guides pass through the second pressing plate. The second liquid guides surround the second liquid inlet channel. The second liquid guides comprise a third plug and a fourth plug. The third plug and the fourth plug are respectively located on two sides of the second pressing plate opposite to each other. The third plug is used to communicate with the liquid inlet of the sample column. The fourth plug is used to communicate with the liquid supply device. N is an integer greater than or equal to 1.

[0017] In an embodiment, the second pressing plate module further comprises a second guide block and a second buffer assembly, the second guide block is arranged on a side of the second pressing plate facing away from the fourth plug, N second guide through holes are formed in the second guide block, and the second guide through holes are arranged opposite to the third plugs one by one; the second buffer assembly is arranged between the second guide block and the second pressing plate, and the second buffer assembly is used to move the second guide block relative to the second pressing plate, so that the third plugs pass through the second guide through holes; the second buffer assembly comprises a third elastic member and a third guide column, one of the second pressing plate and the second guide block is fixedly connected with one end of the third guide column, and the other is slidably connected with the other end of the third guide column, the third elastic member surrounds the third guide column, one end of the third elastic member abuts against the second pressing plate, and the other end of the third elastic member abuts against the second guide block.

[0018] In an embodiment, the over-column device further comprises a second plug pulling module, the second plug pulling module is connected with the first pressing plate module, and the second plug pulling module is used to pull down a lower plug located at a liquid outlet of the over-column device.

[0019] In an embodiment, the over-column device further comprises a first plug pulling module, the first plug pulling module is used to pull down a lower plug located at a liquid outlet of the over-column device; the over-column device further comprises a base, a vertical plate and a second driving module, the first plug pulling module and the docking module are both mounted on the base; the vertical plate is connected with the base, the first pressing plate module and the second pressing plate module are both slidably connected with the vertical plate, and the first driving module is arranged on the base or the vertical plate; the second driving module is arranged on the base or the vertical plate, the second driving module is connected with the second pressing plate module, and the second driving module is used to drive the second pressing plate module to move close to or away from the first pressing plate module.

[0020] In an embodiment, the over-column device further comprises a positioner, the positioner is mounted on the base or the vertical plate, and the positioner is used to position the chromatographic column and / or the sample column.

[0021] In an embodiment, the over-column device further comprises a carrying mechanism, the carrying mechanism is used to carry the chromatographic column and the sample column, and the carrying mechanism is also used to assist in pulling down lower plugs of the chromatographic column and the sample column.

[0022] In a second aspect, the utility model provides an experimental equipment, including liquid supply device, liquid receiving device and the over-column device of as described in first aspect, over-column device is connected with liquid supply device and liquid receiving device respectively.

[0023] The over column device provided by the utility model can be applied to the system of automatic over column, the over column device includes the docking module and the first pressing plate module for fixing and guiding liquid, and the first driving module for driving the first pressing plate module, through the docking module and the first pressing plate module, not only the chromatographic column can be fixed and guided, but also the liquid receiving device and the liquid supply device can be connected respectively, the distance between the first pressing plate module and the docking module can be dynamically adjusted according to the specification of the chromatographic column. It can be seen that the over column device improves the automation degree of over column operation, reduces the labor cost, and improves the compatibility of the device; the automatic over column process greatly improves the sample processing speed, so that a large number of samples can be processed in a short time, time is saved, human error is reduced, and the repeatability and accuracy of the experiment are improved; the efficiency, accuracy and safety of the experiment are improved, and the laboratory work is more efficient and standardized. BRIEF DESCRIPTION OF DRAWINGS

[0024] In order to more clearly illustrate the technical scheme in the embodiments of the utility model or the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below, and obviously, the drawings in the following description are only some embodiments of the utility model, and those skilled in the art can also obtain other drawings according to these drawings without creating labor.

[0025] Figure 1 It is the front view of the over column device of an embodiment;

[0026] Figure 2 It is the back view of the over column device of an embodiment;

[0027] Figure 3 It is the connection schematic view of the over column device, the liquid supply device and the liquid receiving device of an embodiment;

[0028] Figure 4 It is the structure diagram of the first plug module and the sectional schematic view of the spring plunger of an embodiment;

[0029] Figure 5 It is the structure diagram of the first plug module and the docking module of an embodiment;

[0030] Figure 6 It is the structure diagram of the first pressing plate module and the second plug module of an embodiment;

[0031] Figure 7 It is the structure diagram of the second plug module and the second pressing plate module of an embodiment;

[0032] Figure 8 It is the schematic view of the over column device including the carrying mechanism of an embodiment.

[0033] BRIEF DESCRIPTION OF DRAWINGS

[0034] 1000 - column passing device;

[0035] 100 - first plug pulling module, 110 - fixed assembly, 111 - mounting seat, 112 - clamping plate, 1121 - clamping groove, 113 - linear bearing, 114 - fixed nut, 120 - movable assembly, 121 - support plate, 122 - spring plunger, 1221 - fixed pin, 1222 - movable pin, 1223 - first elastic member, 123 - first guide column, 124 - liquid guide plate, 1241 - flow guide surface, 130 - bottom plate, 140 - vertical plate;

[0036] 200 - docking module, 210 - support seat, 220 - liquid inlet connector group, 2201 - first connector, 230 - liquid outlet connector group, 2301 - second connector,

[0037] 300 - first pressing plate module, 310 - first pressing plate, 320 - first liquid guide member, 3201 - first plug, 3202 - second plug, 330 - first guide block, 3301 - first guide through hole, 340 - first buffer assembly, 341 - second elastic member, 342 - second guide column;

[0038] 400 - first driving module, 410 - first transmission member, 420 - first driving member;

[0039] 500 - second pressing plate module, 510 - second pressing plate, 520 - second liquid guide member, 5201 - third plug, 5202 - fourth plug, 530 - second guide block, 5301 - second guide through hole, 540 - second buffer assembly, 541 - third elastic member, 542 - third guide column;

[0040] 600 - second driving module, 610 - second transmission member, 620 - second driving member;

[0041] 700 - second plug pulling module, 710 - liquid receiving groove, 720 - containing bin;

[0042] 800 - support module, 810 - base, 820 - vertical plate;

[0043] 910 - positioner, 920 - carrying mechanism;

[0044] 2000 - liquid supply device, 3000 - liquid receiving device, 4000 - chromatographic column, 5000 - sample column. DETAILED DESCRIPTION

[0045] With reference to the accompanying drawings, the technical solutions in the embodiments of the present application will be described clearly and completely. Obviously, the described embodiments are only part of the embodiments of the present application, but not all the embodiments. Based on the embodiments of the present application, all the other embodiments obtained by those skilled in the art without creative work are within the scope of the present application.

[0046] It should be noted that when a module is referred to as being "fixed to" another module, it can be directly on the other module or there can be intervening modules. When a module is referred to as being "connected to" another module, it can be directly connected to the other module or there can be intervening modules.

[0047] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used in the description of the application herein is for the purpose of describing one embodiment only and is not intended to be limiting of the application. The use herein of "including," "comprising," "having," "containing," "involving," and variations thereof, is meant to encompass the items listed thereafter and equivalents thereof as well as additional items.

[0048] Some embodiments of the present application will be described in detail below with reference to the accompanying drawings. The following examples and features in the examples can be combined with each other in the case of no conflict.

[0049] The present application provides a kind of over column device 1000, please refer to Figures 1-8 .

[0050] In an embodiment, please refer to Figures 1-3 , over column device 1000 includes docking module 200, first pressing plate module 300 and first drive module 400. Wherein, docking module 200 is used to connect the liquid outlet of chromatographic column 4000 and liquid receiving device 3000;First pressing plate module 300 is oppositely arranged with docking module 200, and first pressing plate module 300 has first liquid inlet channel, and one end of first liquid inlet channel is used to connect the liquid inlet of chromatographic column 4000, and the other end of first liquid inlet channel is used to connect liquid supply device 2000;First drive module 400 is connected with first pressing plate module 300, and first drive module 400 drives first pressing plate module 300 to be close to or away from docking module 200.

[0051] In one embodiment, the chromatographic column 4000 is used to separate and purify sample components. The chromatographic column 4000 is a tubular experimental tool, which includes an inlet port for liquid inlet and an outlet port for liquid outlet along the axial direction of the chromatographic column 4000. The chromatographic column 4000 is placed between the docking module 200 and the first platen module 300. The docking module 200 and the first platen module 300 are used to secure and conduct the chromatographic column 4000. The first platen module 300 is also used to connect the liquid supply device 2000, which is used to supply liquid to the chromatographic column 4000 through the first platen module 300. The docking module 200 is also used to connect the liquid receiving device 3000, which is used to receive sample components eluted from the chromatographic column 4000.

[0052] In one embodiment, the first platen module 300 has a first liquid inlet channel, which is in communication with the liquid storage of the liquid supply device 2000 after the liquid supply device 2000 is connected to the first platen module 300. It is to be understood that the liquid storage of the liquid supply device 2000 can be loaded with mixed sample and eluent, which are used to separate and purify sample components in the chromatographic column 4000 through the liquid supply device 2000 and the first platen module 300.

[0053] In one embodiment, please refer to Figure 2 The first driving module 400 can include a first transmission member 410 and a first driving member 420, wherein the first transmission member 410 connects the first driving member 420 and the first platen module 300. The first driving member 420 can be a pneumatic cylinder or an electric motor, but is not limited thereto. The first driving member 420 can be used to provide power for the lifting of the first platen module 300, so as to drive the first platen module 300 to approach or move away from the docking module 200. In specific embodiments, the first driving module 400 can further include a tank chain, which is used to constrain the magnetic switch wiring of the first driving member 420.

[0054] The column passing device 1000 provided by the utility model can be applied to a system of automatic column passing, the column passing device 1000 comprises a docking module 200 and a first pressing plate module 300 for fixing and guiding liquid, and a first driving module 400 for driving the first pressing plate module 300, the docking module 200 and the first pressing plate module 300 can not only fix and guide the chromatographic column 4000, but also can be connected with a liquid receiving device 3000 and a liquid supply device 2000 respectively, and the first driving module 400 can dynamically adjust the distance between the first pressing plate module 300 and the docking module 200 according to the specification of the chromatographic column 4000. It can be seen that the column passing device 1000 improves the automation degree of column passing operation, reduces the labor cost, and improves the compatibility of the device; the automatic column passing process greatly improves the sample processing speed, so that a large number of samples can be processed in a short time, time is saved, human error is reduced, and the repeatability and accuracy of the experiment are improved; the efficiency, accuracy and safety of the experiment are improved, and the laboratory work is more efficient and standardized.

[0055] In one embodiment, referring to Figure 1 The column passing device 1000 further comprises a first plug pulling module 100 for pulling off the lower plug at the outlet of the chromatographic column 4000.

[0056] In one embodiment, before the conventional column passing operation, the outlet of the chromatographic column 4000 is blocked by a lower plug arranged at the outlet, so as to prevent the substances in the chromatographic column 4000 from flowing out. Therefore, the column passing device 1000 comprises a first plug pulling module 100 for pulling off the lower plug at the outlet of the chromatographic column 4000. After the lower plug is pulled off, the inlet and the outlet of the chromatographic column 4000 are connected with the outside.

[0057] In one embodiment, referring to Figure 4 The first plug pulling module 100 comprises a fixed component 110 and a movable component 120, the fixed component 110 is slidably connected with the movable component 120, a clamping groove 1121 is formed in the fixed component 110, and the movable component 120 is used for abutting against the lower plug of the chromatographic column 4000 and sliding relative to the fixed component 110, so that the lower plug of the chromatographic column 4000 is clamped into the clamping groove 1121.

[0058] In one embodiment, the first plug module 100 is composed of a fixed assembly 110 and a movable assembly 120, wherein the movable assembly 120 is movably connected with the fixed assembly 110, that is, the movable assembly 120 can move relative to the fixed assembly 110, and the moving mode is not limited to sliding, and the two can also be rotatably connected. The fixed assembly 110 is provided with a clamping groove 1121 or a clamping hole. Taking the clamping groove 1121 as an example, the clamping groove 1121 can be a groove structure adapted to the outer peripheral contour of the lower plug. In specific embodiments, the shape of the lower plug can be cylindrical or conical, so the clamping groove 1121 is circular. Of course, the shape of the lower plug can also be polygonal or elliptical, and the clamping groove 1121 can also be polygonal or elliptical. It should be noted that the caliber of the clamping groove 1121 can be slightly smaller than the outer diameter of the lower plug, that is, the lower plug is slightly larger than the clamping groove 1121, so that the lower plug can be clamped by the clamping groove 1121 after being inserted into the clamping groove 1121.

[0059] In specific embodiments, the sliding connection between the fixed assembly 110 and the movable assembly 120 is to facilitate the fixed assembly 110 to pull out the lower plug of the chromatographic column 4000. After the chromatographic column 4000 is placed on the first plug module 100, the lower plug of the chromatographic column 4000 contacts the movable assembly 120, and the lower plug pushes the movable assembly 120 to slide relative to the fixed assembly 110 until the lower plug can be clamped into the clamping groove 1121. Preferably, the chromatographic column 4000 pushes the movable assembly 120 downward along the direction of gravity, so that the movable assembly 120 moves downward along the direction of gravity, thereby inserting the lower plug into the clamping groove 1121 and clamping the lower plug by the clamping groove 1121, so that the lower plug can be pulled out by the force when pulling out the chromatographic column 4000.

[0060] The utility model discloses a first plug module 100 is set up fixed assembly 110 and movable assembly 120 in, utilize the slidable relation of fixed assembly 110 and movable assembly 120, the lower plug of chromatographic column 4000 is clamped into the clamping groove 1121 of fixed assembly 110, utilize fixed assembly 110 to hold the lower plug, thereby can smoothly pull out the lower plug from the liquid outlet.

[0061] In one embodiment, please refer to Figure 4 The fixed assembly 110 includes a mounting seat 111 and a clamping plate 112, wherein the movable assembly 120 is arranged on the mounting seat 111 and is slidably connected with the mounting seat 111; the clamping plate 112 is arranged in a spaced-apart manner relative to the mounting seat 111, the movable assembly 120 is arranged between the mounting seat 111 and the clamping plate 112, and the clamping groove 1121 penetrates through the clamping plate 112 along the thickness direction of the clamping plate 112.

[0062] In one embodiment, the mounting base 111 is configured to support the movable assembly 120, and the clamping plate 112 is configured to clamp the lower end plug of the chromatographic column 4000, so as to facilitate the chromatographic column 4000 to be separated from the lower end plug on the liquid outlet thereof. The mounting base 111 and the clamping plate 112 are arranged in opposite directions in the axial direction of the chromatographic column 4000 (generally in the direction of gravity), and the movable assembly 120 is arranged between the mounting base 111 and the clamping plate 112. The mounting base 111 is configured to support the movable assembly 120, and at least part of the movable assembly 120 is configured to slide relative to the mounting base 111. In a specific embodiment, at least part of the movable assembly 120 is configured to slide relative to the mounting base 111 in the axial direction of the chromatographic column 4000.

[0063] In one embodiment, please refer to Figure 4 The clamping plate 112 can be in the shape of a rectangular plate, and two opposite surfaces of the clamping plate 112 are respectively configured to face and be away from the mounting base 111. The clamping groove 1121 is arranged at the edge of the clamping plate 112 and extends into the clamping plate 112, and the clamping groove 1121 further penetrates the two opposite surfaces of the clamping plate 112. In this way, when the lower end plug of the chromatographic column 4000 is inserted into the clamping groove 1121, the lower end plug and the movable assembly 120 abut each other, and the movable assembly 120 is pressed to slide, and the lower end plug is firmly clamped in the clamping groove 1121. In other embodiments, the clamping plate 112 can also have other shapes, such as an elliptical shape, a circular shape, etc. The clamping groove 1121 can also be replaced by a clamping hole arranged in the middle of the clamping plate 112, and the clamping hole penetrates the two opposite surfaces of the clamping plate 112.

[0064] The mounting base 111 is configured to support the movable assembly 120, and the clamping plate 112 is configured to clamp the lower end plug, so that the lower end plug can be clamped into the clamping groove 1121 during the process of the chromatographic column 4000 pressing the movable assembly 120, and the lower end plug is kept in an upright position by the abutting of the movable assembly 120, so as to facilitate the lower end plug to be restored after the chromatographic column 4000 is separated.

[0065] In one embodiment, please refer to Figure 4The active assembly 120 comprises a support plate 121, a spring plunger 122 and a first guide column 123, wherein the support plate 121 is arranged between the mounting base 111 and the clamping plate 112; the spring plunger 122 comprises a fixed pin 1221, a movable pin 1222 and a first elastic member 1223, the fixed pin 1221 is arranged through the mounting base 111 and is fixedly connected with the mounting base 111, the movable pin 1222 is connected with the support plate 121, and opposite ends of the first elastic member 1223 are fixedly connected with the fixed pin 1221 and the movable pin 1222, respectively, and the first elastic member 1223 is elastically deformed along the thickness direction of the support plate 121; the first guide column 123 is arranged through the mounting base 111 and is slidingly connected with the mounting base 111, the first guide column 123 is arranged in parallel with the spring plunger 122, one end of the first guide column 123 is connected with the support plate 121, and the other end of the first guide column 123 is a free end.

[0066] In an embodiment, the support plate 121 is used for supporting the lower plug, the clamping plate 112, the support plate 121 and the spring plunger 122 jointly act to keep the pulled-out plug in a vertical posture, thereby facilitating the plug to be returned after the column separation of the chromatographic column 4000 is completed. The spring plunger 122 is used for providing an upward thrust to make the lower plug clamped into the clamping plate 112. The support plate 121 can be a rectangular plate, and opposite two large faces of the support plate 121 are respectively directed to the clamping plate 112 and the mounting base 111. When the lower plug of the chromatographic column 4000 does not abut against the support plate 121, part of the large face of the support plate 121 can be connected with part of the large face of the clamping plate 112. Two through holes are arranged on the mounting base 111, the fixed pin 1221 is inserted into one of the through holes and fixed therein, the first guide column 123 is inserted into the other through hole, the lower end of the first guide column 123 is suspended, and the first guide column 123 can slide relative to the mounting base 111. In a specific embodiment, the first elastic member 1223 can be a compression spring, and opposite two ends of the first elastic member 1223 are connected with the fixed pin 1221 and the movable pin 1222, respectively, and the first elastic member 1223 can be elastically deformed along the axial direction of the first guide column 123.

[0067] When the lower plug of the chromatographic column 4000 abuts against the support plate 121, the support plate 121 is stressed and deformed by the first elastic member 1223 under the pressing of the movable pin 1222, and the support plate 121 can move a distance. At the same time, the first elastic member 1223 generates an upward elastic potential energy. When the chromatographic column 4000 is moved away and the pressing force is small, the first elastic member 1223 pushes the movable pin 1222 and the support plate 121 to abut against the lower plug upward, so that the pulled-out plug is kept in a vertical posture, thereby facilitating the plug to be returned after the column separation of the chromatographic column 4000 is completed.

[0068] In an embodiment, please refer to Figure 4The fixed assembly 110 further comprises a linear bearing 113 and two fixed nuts 114, wherein the linear bearing 113 is arranged in a through hole of the mounting base 111, the first guide column 123 extends into the linear bearing 113 and is in sliding connection with the linear bearing 113, and the linear bearing 113 is used for assisting the up-down movement of the first guide column 123 to be smoother, and the two fixed nuts 114 are respectively arranged on the two opposite sides of the mounting base 111, and the two fixed nuts 114 are used for fixing the fixed pin 1221 arranged in the through hole.

[0069] In specific embodiments, the fixed pin 1221 is arranged in a through hole of the mounting base 111, and two ends of the fixed pin 1221 extend out of the through hole, and the two ends of the fixed pin 1221 extending out of the through hole are provided with threaded structures matched with the nuts, so that the fixed pin 1221 is fixed on the mounting base 111 by the up-down locking fixed nuts 114.

[0070] The linear bearing 113 can assist the up-down movement of the first guide column 123 to be smoother, and the two fixed nuts 114 can reinforce the fixed pin 1221, so that the stability of the spring plunger 122 can be improved.

[0071] In one embodiment, please refer to Figure 4 The movable assembly 120 further comprises a liquid guide plate 124, the liquid guide plate 124 is arranged between the mounting base 111 and the support plate 121, and the liquid guide plate 124 is connected with the support plate 121; the liquid guide plate 124 comprises a flow guide surface 1241, the flow guide surface 1241 is arranged around the edge of the support plate 121 and protrudes from the support plate 121, the flow guide surface 1241 and the support plate 121 have an included angle therebetween, and the flow guide surface 1241 extends from the support plate 121 in a direction away from the mounting base 111.

[0072] In one embodiment, the liquid guide plate 124 is used for protecting and guiding the leaked liquid of the chromatographic column 4000 after the lower plug is pulled out, so as to avoid that the linear bearing 113 and the spring plunger 122 are corroded by the organic solvent. The liquid guide plate 124 can be a rectangular plate, and the two opposite surfaces of the liquid guide plate 124 are respectively directed to the support plate 121 and the mounting base 111.

[0073] In one embodiment, the movable pin 1222 is connected with the support plate 121 through the liquid guide plate 124, and the movable pin 1222 is fixedly connected with the liquid guide plate 124 and the support plate 121. In other embodiments, the movable pin 1222 can be fixedly connected with the liquid guide plate 124, and the liquid guide plate 124 can be fixedly connected with the support plate 121.

[0074] In an embodiment, the first guide column 123 is connected with the support plate 121 through the liquid guide plate 124, and the first guide column 123 is fixedly connected with the liquid guide plate 124, and the first guide column 123 is also fixedly connected with the support plate 121. In other embodiments, the first guide column 123 can be fixedly connected with the liquid guide plate 124, and the liquid guide plate 124 is fixedly connected with the support plate 121.

[0075] In an embodiment, the liquid guide plate 124 is provided with a flow guide surface 1241 around the periphery, the flow guide surface 1241 faces the clamping plate 112, and the flow guide surface 1241 and the support plate 121 (or the clamping plate 112) form a non-zero angle. In a specific embodiment, the flow guide surface 1241 can be polished on the four edges of the large surface of the liquid guide plate 124 facing the support plate 121, that is, the flow guide surface 1241 and the large surface of the liquid guide plate 124 are connected and form an angle. Alternatively, in other embodiments, the liquid guide plate 124 can be provided with a flow guide portion around the periphery, and the flow guide surface 1241 is provided on the flow guide portion. Alternatively, in other embodiments, the liquid guide plate 124 is only provided with the flow guide surface 1241, and the flow guide surface 1241 is arranged around the periphery of the support plate 121.

[0076] The utility model discloses a liquid guide plate 124 with a flow guide surface 1241, which can guide the organic solvent flowing out after the plug is pulled to other positions, thereby avoiding the corrosion of the organic solvent to the parts in the device and affecting the service life of the device.

[0077] In an embodiment, please refer to Figure 4 , the first plug module 100 further comprises a bottom plate 130 and a vertical plate 140, the vertical plate 140 is connected with the bottom plate 130, the mounting seat 111 is located between the bottom plate 130 and the clamping plate 112, and the mounting seat 111 is arranged in opposite and spaced apart from the bottom plate 130, and the clamping plate 112 is connected with the vertical plate 140.

[0078] In an embodiment, the mounting seat 111 is arranged in opposite and spaced apart from the bottom plate 130, so as to provide space for the movement of the first guide column 123 and also provide space for the installation of the spring plunger 122. In a specific embodiment, the mounting seat 111 can be fixed on the bottom plate 130 through an adapter plate, and the mounting seat 111 and the adapter plate jointly form a "T" shape, and the adapter plate can provide a certain height for the mounting seat 111, so as to adapt to the distance between the mounting seat 111 and the clamping plate 112. In other specific embodiments, the mounting seat 111 can also be fixed on the vertical plate 140.

[0079] In an embodiment, the vertical plate 140 and the bottom plate 130 are connected at an angle, preferably, the vertical plate 140 is vertically erected on the bottom plate 130, and the clamping plate 112 is connected to a side of the vertical plate 140 away from the bottom plate 130. The bottom plate 130 is provided with a mounting groove, preferably, the mounting groove extends in a straight line. The mounting seat 111 is mounted in the mounting groove through the adapter plate.

[0080] In an embodiment, please refer to Figure 5 The number of the fixing assembly 110 is multiple groups, and the mounting seat 111 of each of the multiple groups of the fixing assembly 110 is clamped in the mounting groove. The number of the fixing assembly 110 and the number of the movable assembly 120 are both multiple groups, and the number of the fixing assembly 110 is the same as the number of the movable assembly 120, so that each of the multiple groups of the fixing assembly 110 is connected with a corresponding group of the movable assembly 120. The multiple groups of the fixing assembly 110 are sequentially arranged along the extension direction of the mounting groove. By arranging the multiple groups of the fixing assembly 110 and the movable assembly 120, the operation of removing the lower plug of the multiple chromatographic columns 4000 can be realized, and the experimental throughput can be improved.

[0081] In an embodiment, please refer to Figure 5 The docking module 200 includes a support seat 210, a liquid inlet connector group 220, and a liquid outlet connector group 230. The liquid inlet connector group 220 is arranged on the support seat 210, and the liquid inlet connector group 220 includes N first connectors 2201, the first connector 2201 is used for connecting the liquid outlet of the chromatographic column 4000, and N is an integer greater than or equal to 1; the liquid outlet connector group 230 is arranged on the support seat 210, and the liquid outlet connector group 230 includes N second connectors 2301, the second connector 2301 and the first connector 2201 are one-to-one corresponding and connected, and the second connector is also used for connecting the liquid connection device 3000.

[0082] In an embodiment, the support seat 210 includes a top surface and a side surface, and the top surface of the support seat 210 faces the first pressing plate module 300. After the lower plug of the chromatographic column 4000 is removed, the chromatographic column 4000 is placed on the top surface of the support seat 210, and preferably, the top surface of the support seat 210 is parallel to the large surface of the support plate 121, so that the chromatographic column 4000 only needs to be moved in parallel to the support seat 210 after the lower plug is removed.

[0083] In an embodiment, when N is greater than or equal to 2, the N first connectors 2201 are arranged on the support base 210 at intervals, and the N first connectors 2201 can have the same or different specifications. The specifications can be different in that the diameters of the N first connectors 2201 are different, so that the chromatographic column 4000 with different outlet diameters can be used. Of course, the plurality of first connectors 2201 with different specifications can improve the compatibility of the column passing device 1000, and the plurality of first connectors 2201 with the same specification can improve the experimental throughput. The plurality of first connectors 2201 are connected to the top surface of the support base 210, so that after the chromatographic column 4000 is placed on the support base 210, the first connector 2201 can be connected to the outlet of the chromatographic column 4000, so as to guide the organic solvent in the chromatographic column 4000 out.

[0084] In an embodiment, when N is greater than or equal to 2, the N second connectors 2301 are arranged on the support base 210 at intervals, and the plurality of second connectors 2301 included in the outlet connector group 230 can have the same or different specifications, and preferably the plurality of second connectors 2301 have the same specification, so as to adapt to the pipeline of the liquid connection device 3000. The plurality of second connectors 2301 are connected to the side surface of the support base 210, and one end of the second connector 2301 is in communication with the first connector 2201. In a specific embodiment, the number of first connectors 2201 can be two, and the number of second connectors 2301 can be two, wherein one first connector 2201 is in communication with one second connector 2301, and the other first connector 2201 is in communication with the other second connector 2301.

[0085] The utility model discloses a butt joint module 200 including support base 210, liquid inlet connector group 220 and liquid outlet connector group 230, on the one hand, the stability of the chromatographic column 4000 placed on the butt joint module 200 can be provided, and the separated sample components can be collected through the liquid outlet connector group 230 in communication with the liquid connection device 3000. And the liquid inlet connector group 220 and the liquid outlet connector group 230 are provided with a plurality of connectors, which can adapt to chromatographic columns 4000 of different specifications.

[0086] The first plug module 100 is arranged close to the butt joint module 200, which can quickly butt joint the outlet of the chromatographic column 4000 with the first connector 2201 of the butt joint module 200 after the lower plug of the chromatographic column 4000 is pulled out, and quickly insert the lower plug of the chromatographic column 4000 into the first plug module 100 after the chromatographic column 4000 is completely passed through the column, so as to avoid the pollution of the device caused by the liquid drop of the outlet of the chromatographic column 4000 due to long-distance movement.

[0087] In an embodiment, please refer to Figure 6The first pressing plate module 300 comprises a first pressing plate 310 and N first liquid guides 320. The first liquid guides 320 are arranged on the first pressing plate 310, and the first liquid guides 320 enclose a first liquid inlet channel. The first liquid guides 320 comprise a first plug 3201 and a second plug 3202. The first plug 3201 and the second plug 3202 are respectively arranged on two opposite sides of the first pressing plate 310. The first plug 3201 is used for connecting the liquid inlet of the chromatographic column 4000, and the second plug 3202 is used for connecting the liquid supply device 2000. N is an integer greater than or equal to 1.

[0088] In an embodiment, the first pressing plate 310 is arranged opposite to the support seat 210. When N is greater than or equal to 2, the number of the first liquid guides 320 is plural, and the plural first liquid guides 320 are arranged one-to-one corresponding to the plural first connectors 2201 in the liquid inlet connector group 220. In a specific embodiment, the number of the first liquid guides 320 can be two, and the number of the first connectors 2201 can be two. One of the first liquid guides 320 is arranged opposite to one of the first connectors 2201, and the other of the first liquid guides 320 is arranged opposite to the other of the first connectors 2201. It should be noted that the plural first liquid guides 320 can be different in specification. In an embodiment, the plural first liquid guides 320 are different in diameter, so as to be applicable to chromatographic columns 4000 with different liquid inlet diameters. The plural first liquid guides 320 can also be the same in specification, so as to simultaneously connect plural chromatographic columns 4000 with the same liquid inlet diameter, thereby improving the experimental throughput.

[0089] In an embodiment, the first liquid guides 320 are arranged on the first pressing plate 310, and the first liquid guides 320 enclose the first liquid inlet channel. The two ends of the first liquid guides 320 extend from two opposite surfaces of the first pressing plate 310, so as to form the first plug 3201 and the second plug 3202. The first plug 3201 is used for connecting the liquid inlet of the chromatographic column 4000, and the second plug 3202 is used for connecting the liquid supply device 2000. The first plugs 3201 in different first liquid guides 320 can be the same or different in specification, and the second plugs 3202 in different first liquid guides 320 can be the same or different in specification, which are not limited herein.

[0090] In an embodiment, please refer to Figure 6The first pressing plate module 300 further comprises a first guide block 330 and a first buffer assembly 340. The first guide block 330 is arranged on a side of the first pressing plate 310 opposite to the second plug 3202. The first guide block 330 is provided with N first guide through holes 3301, and each first guide through hole 3301 is arranged opposite to a first plug 3201. The first buffer assembly 340 is arranged between the first guide block 330 and the first pressing plate 310. The first buffer assembly 340 is used to move the first guide block 330 relative to the first pressing plate 310, so that the first plug 3201 passes through the first guide through hole 3301. The first buffer assembly 340 comprises a second elastic member 341 and a second guide column 342. One of the first pressing plate 310 and the first guide block 330 is fixedly connected with one end of the second guide column 342, and the other is slidingly connected with the other end of the second guide column 342. The second elastic member 341 surrounds the second guide column 342, and one end of the second elastic member 341 abuts against the first pressing plate 310, and the other end of the second elastic member 341 abuts against the first guide block 330.

[0091] In an embodiment, the first guide block 330 is arranged between the first pressing plate 310 and the support seat 210, and the first guide block 330 is connected with the first pressing plate 310 through the first buffer assembly 340. The first guide block 330 is provided with at least one first guide through hole 3301, and the first guide through hole 3301 is used for the first plug 3201 to pass through. In the case where the chromatographic column 4000 is not inserted, the first plug 3201 can be located above the first guide through hole 3301. When the chromatographic column 4000 is inserted, the chromatographic column 4000 extrudes the first guide block 330 upwards, so that the first plug 3201 passes through the first guide through hole 3301 and is connected with the liquid inlet of the chromatographic column 4000.

[0092] It can be understood that the first guide block 330 is elastically connected with the first pressing plate 310 through the first buffer assembly 340, and the first guide block 330 is used to guide the chromatographic column 4000 upwards when the chromatographic column 4000 is installed between the first pressing plate module 300 and the docking module 200, so as to improve the docking success rate. In a specific embodiment, the first guide block 330 is provided with the first guide through hole 3301 at a position opposite to the first connector 2201. When the chromatographic column 4000 is slightly inclined, the first guide through hole 3301 can guide the chromatographic column 4000 to be correct, so that the liquid inlet of the chromatographic column 4000 is accurately connected with the first plug 3201.

[0093] In an embodiment, the second elastic member 341 can be a compression spring, a rubber sleeve, a spring sheet or the like. The second elastic member 341 is preferably a compression spring. One end of the second guide column 342 is fixedly connected with the first pressing plate 310, and the other end of the second guide column 342 is provided through the first guide block 330 and is in sliding connection with the first guide block 330. The second elastic member 341 is provided around the second guide column 342, and the two ends of the second elastic member 341 away from each other abut against the first pressing plate 310 and the first guide block 330, respectively. In other embodiments, the second guide column 342 can be in sliding connection with the first pressing plate 310 and in fixed connection with the first guide block 330. Therefore, when the chromatographic column 4000 presses the first guide block 330, the second elastic member 341 is compressed under force and generates elastic potential energy, so that the second elastic member 341 is used to provide a reverse thrust for the first guide block 330, so that the first guide block 330 presses the chromatographic column 4000 tightly.

[0094] In an embodiment, referring to Figure 7 The column passing device 1000 further comprises a second pressing plate module 500, the second pressing plate module 500 is arranged opposite to the first pressing plate module 300, the other end of the first liquid inlet channel is used for connecting the liquid outlet of the sample column 5000, the second pressing plate module 500 has a second liquid inlet channel, one end of the second liquid inlet channel is used for connecting the liquid inlet of the sample column 5000, and the other end of the second liquid inlet channel is used for connecting the liquid supply device 2000.

[0095] In an embodiment, the sample column 5000 is used for accommodating a sample to be separated and purified, and is a tubular experimental tool. Along the axial direction of the sample column 5000, the sample column 5000 comprises a liquid inlet for liquid inlet and a liquid outlet for liquid outlet. Before a conventional column passing operation, the liquid outlet of the sample column 5000 is blocked by a lower plug arranged at the liquid outlet, so as to avoid the outflow of substances in the sample column 5000. The sample in the sample column 5000 is flushed into the chromatographic column 4000 by the eluent provided by the liquid supply device 2000. The second pressing plate module 500 is used for connecting the liquid inlet of the sample column 5000 with the liquid supply device 2000, and the second pressing plate module 500 and the first pressing plate module 300 further function as fixing the sample column 5000.

[0096] The utility model discloses through setting up one second pressing plate module 500 again, using second pressing plate module 500 and first pressing plate module 300 fixed sample column 5000, and the conduction sample column 5000 and chromatographic column 4000 can be applicable to the scene of dry method sample loading, so great improvement sample processing's speed can be made, and a large number of samples can be handled in a short time, save time, reduce the human error, thereby improve the repeatability and accuracy of experiment.

[0097] In an embodiment, referring to Figure 7The second pressing plate module 500 comprises a second pressing plate 510 and N second liquid guides 520, wherein the second liquid guides 520 are arranged through the second pressing plate 510, the second liquid guides 520 enclose a second liquid inlet channel, the second liquid guides 520 comprise a third plug 5201 and a fourth plug 5202, the third plug 5201 and the fourth plug 5202 are respectively arranged on two opposite sides of the second pressing plate 510, the third plug 5201 is used for connecting the liquid inlet of the sample loading column 5000, the fourth plug 5202 is used for connecting the liquid supply device 2000, and N is an integer greater than or equal to 1.

[0098] In an embodiment, the second pressing plate 510 and the first pressing plate 310 can have the same or similar structure, and the number of the second liquid guides 520 can be the same as that of the first liquid guides 320. When N is greater than or equal to 2, the plurality of second liquid guides 520 are arranged one by one corresponding to the plurality of first liquid guides 320.

[0099] In an embodiment, the second pressing plate 510 module further comprises a second guide block 530 and a second buffer assembly 540. The second guide block 530 is arranged on a side of the second pressing plate 510 opposite to the fourth plug 5202. N second guide through holes 5301 are arranged on the second guide block 530, and the second guide through holes 5301 are arranged one by one opposite to the third plug 5201. The second buffer assembly 540 is arranged between the second guide block 530 and the second pressing plate 510. The second buffer assembly 540 is used for moving the second guide block 530 relative to the second pressing plate 510, so that the third plug 5201 passes through the second guide through hole 5301. The second buffer assembly 540 comprises a third elastic member 541 and a third guide column 542. One of the second pressing plate 510 and the second guide block 530 is fixedly connected with one end of the third guide column 542, and the other is slidingly connected with the other end of the third guide column 542. The third elastic member 541 is arranged around the third guide column 542, one end of the third elastic member 541 abuts against the second pressing plate 510, and the other end of the third elastic member 541 abuts against the second guide block 530. In this embodiment, the second guide block 530 and the second buffer assembly 540 can have the same arrangement as the first guide block and the second buffer assembly 540, and have the same effect. For details, please refer to the first pressing plate module 300, which will not be described here.

[0100] In an embodiment, please refer to Figure 2The column passing device 1000 further comprises a second driving module 600, the second driving module 600 is connected with the second pressing plate module 500, and the second driving module 600 drives the second pressing plate module 500 to be close to or away from the first pressing plate module 300. In an embodiment, the second driving module 600 can comprise a second transmission member 610 and a second driving member 620, wherein the second transmission member 610 is connected between the second driving member 620 and the second pressing plate module 500. The second driving member 620 comprises but is not limited to a pneumatic cylinder or an electric motor. The second driving member 620 can be used to provide power for lifting the second pressing plate module 500, so as to drive the second pressing plate module 500 to be close to or away from the first pressing plate module 300. In a specific embodiment, the second driving module 600 can further comprise a tank chain, and the tank chain is used to constrain the magnetic switch wiring of the second driving member 620.

[0101] In an embodiment, referring to Figure 6 and Figure 7 The column passing device 1000 further comprises a second plug pulling module 700, the second plug pulling module 700 is connected with the first pressing plate module 300, and the second plug pulling module 700 is used to pull off the lower plug located at the liquid outlet of the sample column 5000.

[0102] In an embodiment, the structure of the second plug pulling module 700 can be the same as or similar to that of the first plug pulling module 100, the second plug pulling module 700 also comprises a fixed assembly and a movable assembly, and the fixed assembly of the second plug pulling module 700 comprises a mounting seat and a clamping plate, and the movable assembly of the second plug pulling module 700 comprises a supporting plate, a spring plunger, a first guide column and a liquid guide plate. The arrangement mode of each part in the movable assembly of the second plug pulling module 700 is the same as that of the movable assembly 120 of the first plug pulling module 100, and the functions are the same, and details are not repeated here.

[0103] The second plug pulling module 700 is connected with the first pressing plate module 300, so that the operation of pulling off and plugging off the plug can be completed nearby the fixed position of the sample column 5000, and the sample column 5000 is prevented from being moved for a long distance to cause liquid drop, so that the speed of sample processing is improved, a large number of samples can be processed in a short time, and time is saved.

[0104] In an embodiment, referring to Figure 6 and Figure 7 The mounting seat in the second plug pulling module 700 is connected with the first pressing plate 310, and the mounting seat in the second plug pulling module 700 is provided with a liquid receiving groove 710, and the clamping plate in the second plug pulling module 700 is connected with the mounting seat through a vertical plate.

[0105] In an embodiment, referring to Figure 6 and Figure 7The mounting seat in the second plug module 700 is different from the mounting seat 111 in the first plug module 100. The second plug module 700 does not include a bottom plate, so the mounting seat in the second plug module 700 is not connected to the bottom plate, but is directly connected to the first pressing plate 310. The side of the mounting seat in the second plug module 700 facing the second pressing plate 510 can be provided with a liquid receiving groove 710 for collecting the organic solvent left by the liquid guide plate in the second plug module 700. Meanwhile, the clamping plate in the second plug module 700 is connected to the mounting seat through a vertical plate, so that the clamping plate in the second plug module 700 is arranged opposite to the mounting seat. A waste liquid bottle can be connected below the liquid receiving groove 710, so that the waste liquid in the liquid receiving groove 710 can be timely discharged.

[0106] In an embodiment, referring to Figure 6 and Figure 7 The second plug module 700 can further include a containing space 720, which encloses a containing space, and the mounting seat, the first guide column and the spring plunger of the second plug module 700 are contained in the containing space 720.

[0107] In an embodiment, referring to Figure 1 and Figure 2 The passing column device 1000 further includes a support module 800, which includes a base 810 and a vertical plate 820. The first plug module 100 and the docking module 200 are both mounted on the base 810. The vertical plate 820 is connected to the base 810. The first pressing plate module 300 and the second pressing plate module 500 are both in sliding connection with the vertical plate 820. The first driving module 400 is arranged on the base 810 or the vertical plate 820. The second driving module 600 is arranged on the base 810 or the vertical plate 820.

[0108] In an embodiment, referring to Figure 1 and Figure 2 The base 810 can be plate-shaped. The first plug module 100 and the docking module 200 in the above-mentioned mode are both mounted on the base 810. The vertical plate 820 is erected on the base 810 and is also plate-shaped. The first plug module 100, the docking module 200, the first pressing plate module 300, the second pressing plate module 500 and the second plug module 700 are all arranged on the same side of the vertical plate 820. The first driving module 400 and the second driving module 600 are both arranged on the other side of the vertical plate 820.

[0109] In an embodiment, referring to Figure 1 and Figure 2The vertical plate 820 is provided with a sliding groove penetrating the plate surface, and the sliding groove extends along the height direction of the vertical plate 820. The first pressing plate 310 and the second pressing plate 510 both extend into the sliding groove and are connected with the corresponding first transmission member 410 and the second transmission member 610. The first transmission member 410 is driven by the first driving member 420 to drive the first pressing plate module 300 to slide, and the second transmission member 610 is driven by the second driving member 620 to drive the second pressing plate module 500 to slide. In a specific embodiment, the vertical plate 820 can also be connected with a fixing member, and the fixing member is used to connect the first driving member 420 and the second driving member 620, so as to fix the first driving member 420 and the second driving member 620 on the vertical plate 820 or the base 810.

[0110] In an embodiment, please refer to Figure 1 The column passing device 1000 further comprises a positioner 910, and the positioner 910 is installed on the base 810 or the vertical plate 820. The positioner 910 is used to position the chromatographic column 4000 and / or the sample column 5000. In an embodiment, the positioner 910 can be a positioning camera, and the positioner 910 is used for visual positioning to determine the accurate position of the chromatographic column 4000 and the sample column 5000. The positioning camera is fixedly connected to the vertical plate 140 in the above-mentioned embodiment. In this way, after the chromatographic column 4000 is pulled out of the lower plug by the first plug pulling module 100, the outlet of the chromatographic column 4000 can be quickly positioned by the positioner 910 to accurately dock with the first connector 2201 of the docking module 200, thereby improving the docking success rate.

[0111] In an embodiment, please refer to Figure 1 and Figure 2 The base 810 is provided with two groups of docking modules 200, the vertical plate 820 is provided with two groups of first pressing plate modules 300 and two groups of second pressing plate modules 500, and the vertical plate 820 is provided with two groups of second plug pulling modules 700. That is, two groups of chromatographic columns 4000 can be processed at the same time on the column passing device 1000, thereby improving the work efficiency.

[0112] In an embodiment, please refer to Figure 8 The column passing device 1000 further comprises a carrying mechanism 920, and the carrying mechanism 920 is used to carry the chromatographic column 4000 and the sample column 5000. The carrying mechanism 920 is also used to assist in pulling out the lower plug of the chromatographic column 4000 and the sample column 5000. The carrying mechanism 920 comprises one or more combinations of a mobile robot, a multi-degree-of-freedom mechanical arm, and an XYZ three-axis translation mechanism.

[0113] Based on the column device 1000 provided in the above-mentioned embodiments, the column device 1000 provided by the utility model can have the following advantages: 1) high working efficiency, the automatic column passing and unattended continuous work can be completed through the cooperation of the carrying mechanism 920, which greatly improves the sample processing speed and the laboratory working efficiency; 2) less experimental error, the automatic operation reduces the human intervention, and reduces the sample pollution or inaccurate experimental results caused by operation errors; 3) high repeatability and accuracy, the column device 1000 can automatically run, and the mechanical repetitive work is accurately controlled to ensure the repeatability and accuracy of the experiment, and improve the reliability of the data; 4) wide application range, the column device 1000 can cooperate with different chromatographic columns 4000 and sample columns 5000, and is suitable for the analysis of various complex samples, thereby expanding the application range of the experiment; 5) time saving, the automatic sample processing and column switching reduce the sample preparation and analysis time, so that the researchers can spend more time on data analysis and research work; 6) high safety, the column device 1000 can reduce the risk of direct contact of the experimental personnel with harmful chemicals, and improve the safety of the laboratory; 7) saving of consumables, through accurate control of the amount of reagents and samples, the column device 1000 helps to reduce the waste of consumables; 8) adaptation to high-throughput analysis, for the laboratory needing to process a large number of samples, the column device 1000 can meet the demand of high-throughput analysis; 9) easy integration and upgrading, the column device 1000 has the modularization characteristics, and the design has good compatibility, so that the column device 1000 can be integrated with the existing chromatographic system, or be upgraded and expanded according to the needs; 10) space saving, compared with the traditional manual operation equipment, the column device 1000 is smaller in size, and saves the laboratory space.

[0114] The utility model also provides an experimental equipment.

[0115] In an embodiment, please refer to Figure 3 The experimental equipment comprises a liquid supply device 2000, a liquid receiving device 3000 and the column passing device 1000 provided in the above-mentioned embodiments, and the column passing device 1000 is connected with the liquid supply device 2000 and the liquid receiving device 3000 respectively.

[0116] The utility model also provides a use method of the column passing device, which adopts the column passing device in the above-mentioned embodiments and is suitable for a dry sample loading scene.

[0117] In an embodiment, after the experiment starts, the carrying mechanism grabs the chromatographic column and places the chromatographic column on the first plug pulling module, the carrying mechanism compresses the spring plunger by pressing the chromatographic column, and then pushes the lower plug into the clamping groove of the clamping plate to clamp the lower plug; then the carrying mechanism pulls up the chromatographic column to separate the lower plug and the chromatographic column.

[0118] In one embodiment, after the lower plug and the chromatographic column are separated, the chromatographic column is moved to the positioner, the outlet of the chromatographic column is positioned by the positioner, the chromatographic column after positioning is inserted into the first connector of the docking module, and the connection is completed; then the first driving module drives the first pressing plate module to descend, and the first plug on the first liquid guide is connected with the inlet of the chromatographic column.

[0119] In one embodiment, after the first plug is connected with the chromatographic column, the carrying mechanism grabs the sample loading column and places the sample loading column on the second plug pulling module, the carrying mechanism presses the sample loading column downward to compress the spring plunger of the sample loading column, and then the lower plug is pushed into the clamping groove of the clamping plate to clamp the lower plug; then the carrying mechanism pulls up the sample loading column to separate the lower plug and the sample loading column.

[0120] In one embodiment, after the lower plug and the chromatographic column are separated, the chromatographic column is moved to the positioner, the outlet of the chromatographic column is positioned by the positioner, the chromatographic column after positioning is inserted into the first connector of the docking module, and the connection is completed; then the first driving module drives the first pressing plate module to descend, and the first plug on the first liquid guide is connected with the inlet of the chromatographic column.

[0121] In one embodiment, after the third plug is connected with the chromatographic column, the liquid supply device is communicated with the fourth plug on the second liquid guide of the second pressing plate module through the liquid inlet pipeline, and the liquid receiving device is communicated with the second connector on the docking module through the liquid outlet pipeline; the liquid supply device supplies liquid (eluent) into the sample loading column, the liquid is mixed with the sample in the sample loading column, and then flows into the chromatographic column through the first pressing plate module to separate the components, and the separated components flow into the liquid receiving device through the docking module to realize separation and purification of the sample components.

[0122] The utility model further provides another use method of the column passing device, which adopts the column passing device in the above embodiment and is suitable for a wet sample loading scene.

[0123] In one embodiment, the use method only has the chromatographic column and does not have the sample loading column. The chromatographic column can be installed between the docking module and the first pressing plate module, and a liquid guide pipe can be installed between the first pressing plate module and the second pressing plate module to guide the liquid in the liquid supply device into the chromatographic column. Of course, the chromatographic column can also be installed between the second pressing plate module and the first pressing plate module, and a liquid guide pipe can be installed between the first pressing plate module and the docking module to guide the liquid in the liquid supply device into the chromatographic column.

[0124] In the description of the embodiments of the utility model, it needs to explain, the term "center", "upper", "lower", "left", "right", "vertical", "horizontal", "internal", "external" and other index direction or position relation is based on the direction or position relation of the drawing, only is for the convenience of describing the utility model and simplifying the description, and is not instructing or implying the device or element indicated must have a particular direction, with a particular direction structure and operation, therefore cannot be understood as the limitation to the utility model.

[0125] The above disclosed is only a preferred embodiment of the utility model, of course cannot with this to limit the utility model right scope, the person skilled in the art can understand the implementation all or part of the above-mentioned embodiment process, and the equivalent change made by the utility model claim, still belongs to the range covered by the utility model.

Claims

1. A passing column device, characterized by, The application relates to a chromatographic column device. The device comprises: a docking module for connecting a liquid outlet of a chromatographic column and a liquid receiving device; a first pressing plate module arranged opposite to the docking module, the first pressing plate module having a first liquid inlet channel, one end of the first liquid inlet channel being used for connecting a liquid inlet of the chromatographic column, and the other end of the first liquid inlet channel being used for connecting a liquid supply device; 2. The passing column apparatus of claim 1, wherein, a first driving module connected with the first pressing plate module, the first driving module being used for driving the first pressing plate module to move close to or away from the docking module.

3. The passing column apparatus of claim 2, wherein, The device further comprises a first plug pulling module used for pulling down a lower plug located at the liquid outlet of the chromatographic column.

4. The passing column apparatus of claim 3, wherein, The first plug pulling module comprises a fixed component and a movable component, the fixed component is in sliding connection with the movable component, a clamping groove is formed in the fixed component, and the movable component is used for abutting against the lower plug of the chromatographic column and sliding relative to the fixed component, so that the lower plug of the chromatographic column is clamped into the clamping groove. The fixed component comprises: a mounting seat, the movable component is arranged on the mounting seat, and the movable component is in sliding connection with the mounting seat; 5. The passing column apparatus of claim 4, wherein, a clamping plate arranged opposite to the mounting seat, the movable component is arranged between the mounting seat and the clamping plate, and the clamping groove penetrates through the clamping plate along the thickness direction of the clamping plate. The movable component comprises: a supporting plate arranged between the mounting seat and the clamping plate; a spring plunger comprising a fixed pin, a movable pin and a first elastic member, the fixed pin is arranged through the mounting seat and is fixedly connected with the mounting seat, the movable pin is connected with the supporting plate, and the two opposite ends of the first elastic member are respectively connected with the fixed pin and the movable pin, and the first elastic member is elastically deformed along the thickness direction of the supporting plate; 6. The passing column apparatus of claim 5, wherein, a first guide column arranged through the mounting seat and in sliding connection with the mounting seat, the first guide column is arranged in parallel with the spring plunger, one end of the first guide column is connected with the supporting plate, and the other end of the first guide column is a free end. The movable component further comprises: a liquid guide plate arranged between the mounting seat and the supporting plate and connected with the supporting plate; 7. The passing column apparatus of claim 4, wherein, the liquid guide plate comprises a flow guide surface, the flow guide surface is arranged around the edge of the supporting plate and protrudes from the supporting plate, an included angle is formed between the flow guide surface and the supporting plate, and the flow guide surface extends away from the mounting seat from the supporting plate.

8. The passing column apparatus of claim 1, wherein, The first plug pulling module further comprises a bottom plate and a vertical plate, the vertical plate is connected with the bottom plate, the mounting seat is located between the bottom plate and the clamping plate, the mounting seat is arranged opposite to the bottom plate, and the clamping plate is connected with the vertical plate. The docking module comprises: a supporting seat; a liquid inlet connector group arranged on the supporting seat, the liquid inlet connector group comprises N first connectors, the first connectors are used for connecting the liquid outlet of the chromatographic column, and N is an integer greater than or equal to 1; a liquid outlet connector group arranged on the supporting seat, the liquid outlet connector group comprises N second connectors, the second connectors and the first connectors are in one-to-one correspondence and are connected, and the second connectors are further used for connecting the liquid receiving device.

9. The passing column apparatus of claim 1, wherein, The first pressing plate module comprises: a first pressing plate; N first liquid guides, the first liquid guides being arranged in the first pressing plate, the first liquid guides enclosing the first liquid inlet channel, the first liquid guides comprising a first plug and a second plug, the first plug and the second plug being respectively located on two opposite sides of the first pressing plate, the first plug being used for connecting the liquid inlet of the chromatographic column, the second plug being used for connecting the liquid supply device, N being an integer greater than or equal to 1.

10. The passing column apparatus of claim 9, wherein, The first pressing plate module further comprises: a first guide block arranged on a side of the first pressing plate opposite to the second plug, the first guide block being provided with N first guide through holes, the first guide through holes being arranged opposite to the first plug one by one; a first buffer assembly arranged between the first guide block and the first pressing plate, the first buffer assembly being used for moving the first guide block relative to the first pressing plate so that the first plug passes through the first guide through hole; the first buffer assembly comprising a second elastic member and a second guide column, one of the first pressing plate and the first guide block being fixedly connected to one end of the second guide column, and the other being slidingly connected to the other end of the second guide column, the second elastic member being arranged around the second guide column, and one end of the second elastic member abutting against the first pressing plate, and the other end of the second elastic member abutting against the first guide block.

11. The column device according to any one of claims 1 to 10, characterized in that The column device further comprises a second pressing plate module, the second pressing plate module being arranged opposite to the first pressing plate module, the other end of the first liquid inlet channel being used for connecting the liquid outlet of the sample column, the second pressing plate module having a second liquid inlet channel, one end of the second liquid inlet channel being used for connecting the liquid inlet of the sample column, and the other end of the second liquid inlet channel being used for connecting the liquid supply device.

12. The passing column apparatus of claim 11, wherein, The second pressing plate module comprises: a second pressing plate; N second liquid guides, the second liquid guides being arranged in the second pressing plate, the second liquid guides enclosing the second liquid inlet channel, the second liquid guides comprising a third plug and a fourth plug, the third plug and the fourth plug being respectively located on two opposite sides of the second pressing plate, the third plug being used for connecting the liquid inlet of the sample column, and the fourth plug being used for connecting the liquid supply device, N being an integer greater than or equal to 1.

13. The passing column apparatus of claim 12, wherein, The second pressing plate module further comprises: a second guide block arranged on a side of the second pressing plate opposite to the fourth plug, the second guide block being provided with N second guide through holes, the second guide through holes being arranged opposite to the third plug one by one; a second buffer assembly arranged between the second guide block and the second pressing plate, the second buffer assembly being used for moving the second guide block relative to the second pressing plate so that the third plug passes through the second guide through hole; the second buffer assembly comprising a third elastic member and a third guide column, one of the second pressing plate and the second guide block being fixedly connected to one end of the third guide column, and the other being slidingly connected to the other end of the third guide column, the third elastic member being arranged around the third guide column, and one end of the third elastic member abutting against the second pressing plate, and the other end of the third elastic member abutting against the second guide block. A second buffering assembly is arranged between the second guide block and the second pressing plate, and is used to move the second guide block relative to the second pressing plate so that the third plug passes through the second guide through hole; the second buffering assembly comprises a third elastic member and a third guide column, one of the second pressing plate and the second guide block is fixedly connected with one end of the third guide column, and the other is slidably connected with the other end of the third guide column, the third elastic member surrounds the third guide column, and one end of the third elastic member abuts against the second pressing plate, and the other end of the third elastic member abuts against the second guide block.

14. The passing column apparatus of claim 11, wherein, The over-column device further comprises a second plug-removing module, the second plug-removing module is connected with the first pressing plate module, and the second plug-removing module is used to remove the lower plug located at the liquid outlet of the upper sample column.

15. The column device according to any one of claims 2 to 7, wherein The over-column device further comprises a second pressing plate module, the second pressing plate module is arranged opposite to the first pressing plate module, the other end of the first liquid inlet channel is used to communicate with the liquid outlet of the upper sample column, the second pressing plate module has a second liquid inlet channel, one end of the second liquid inlet channel is used to communicate with the liquid inlet of the upper sample column, and the other end of the second liquid inlet channel is used to communicate with the liquid supply device. The over-column device further comprises: A base, the first plug-removing module and the docking module are installed on the base; A vertical plate is connected with the base, the first pressing plate module and the second pressing plate module are slidably connected with the vertical plate, and the first driving module is arranged on the base or the vertical plate; A second driving module is arranged on the base or the vertical plate, the second driving module is connected with the second pressing plate module, and the second driving module is used to drive the second pressing plate module to move close to or away from the first pressing plate module.

16. The column device according to any one of claims 1, 8-10, wherein The over-column device further comprises a first plug-removing module, the first plug-removing module is used to remove the lower plug located at the liquid outlet of the chromatographic column; The over-column device further comprises a second pressing plate module, the second pressing plate module is arranged opposite to the first pressing plate module, the other end of the first liquid inlet channel is used to communicate with the liquid outlet of the upper sample column, the second pressing plate module has a second liquid inlet channel, one end of the second liquid inlet channel is used to communicate with the liquid inlet of the upper sample column, and the other end of the second liquid inlet channel is used to communicate with the liquid supply device. The over-column device further comprises: A base, the first plug-removing module and the docking module are installed on the base; A vertical plate is connected with the base, the first pressing plate module and the second pressing plate module are slidably connected with the vertical plate, and the first driving module is arranged on the base or the vertical plate; A second driving module is arranged on the base or the vertical plate, the second driving module is connected with the second pressing plate module, and the second driving module is used to drive the second pressing plate module to move close to or away from the first pressing plate module.

17. The passing column apparatus of claim 15, wherein, The over-column device further comprises a positioner, the positioner is installed on the base or the vertical plate, and the positioner is used to position the chromatographic column and / or the upper sample column.

18. The passing column apparatus of claim 16, wherein, The over-column device further comprises a positioner mounted on the base or the stand plate, the positioner being used for positioning the chromatographic column and / or the sample column.

19. The passing column apparatus of claim 11, wherein, The over-column device further comprises a carrying mechanism, the carrying mechanism being used for carrying the chromatographic column and the sample column, and the carrying mechanism being further used for assisting in pulling out the lower plug of the chromatographic column and the sample column.

20. An experimental apparatus, characterized by The over-column device comprises a liquid supply device, a liquid receiving device, and the over-column device as claimed in any one of claims 1-19, the over-column device being connected with the liquid supply device and the liquid receiving device respectively.

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