High-throughput protein purification device

Abstract

The utility model relates to a protein high-flux purification device in the technical field of protein purification, which comprises a liquid receiving box, a deep hole plate is sleeved at the upper end of the liquid receiving box, a plurality of deep grooves are arranged at the upper end of the deep hole plate, water outlet holes communicated with the liquid receiving box are arranged on the inner bottom walls of the deep grooves, and a gasket is inserted in the deep hole plate. A pair of rectangular blocks are fixedly connected to a pair of side ends of the deep hole plate respectively; after purification processing, a spray head enters a deep groove, and water is sprayed out of the spray head under the pressure of a rubber plate to flush the inner wall of the deep groove, so that during use, the cleaning steps are simple and time-saving, personnel operation is facilitated, and after cleaning, clean water is sucked into a cover plate to be stored for next cleaning and use; and the deep groove is sealed through the cover plate, so that the interior of the deep groove is not prone to being polluted by dust, and dustproof protection is conducted on the deep groove.

Description

TECHNICAL FIELD

[0001] The utility model relates to protein high throughput purification device, especially related to protein high throughput purification device applied to protein purification field. BACKGROUND

[0002] The significance and purpose of protein purification is to obtain high-purity specific proteins in order to deeply study its properties, functions, and apply to medical, industrial and scientific research and other fields. Protein purification is a basic link in protein research, which has important significance for understanding the law of life activities and elucidating the nature of life phenomena.

[0003] The utility model discloses a protein purification device, including the box, through the setting several gravity chromatography column, can make several gravity chromatography column work simultaneously, can effectively improve the purification efficiency, sets up the top cap at the feed inlet of the box, can effectively prevent other impurities in the air from polluting protein raw materials, the drive motor and the stirring shaft are arranged in the liquid storage cavity, the drive motor drives the stirring shaft to rotate, makes the organic solvent in the liquid storage cavity and protein stock solution fully mix, improves the purification effect, the protein purification device designed by the utility model can improve the purification efficiency, is suitable for the rapid, standardization and the experimental work development in the ELISA laboratory.

[0004] The above-mentioned purification device can mix the organic solvent in the liquid storage cavity and the protein stock solution fully, improve the purification effect, and after use, due to the insufficient internal space, it brings certain inconvenience to the personnel cleaning. Utility model content

[0005] In view of the above prior art, the technical problem to be solved by the utility model is the problem that the existing protein purification device is inconvenient for personnel cleaning.

[0006] To solve the above problems, the utility model provides a protein high throughput purification device, which comprises a liquid receiving tank, a deep hole plate is sleeved on the upper end of the liquid receiving tank, a plurality of deep grooves are formed in the upper end of the deep hole plate, a plurality of water outlets are formed in the inner bottom wall of the deep grooves and communicate with the liquid receiving tank, a gasket is inserted into the deep hole plate, a pair of rectangular blocks are fixedly connected to the pair of side ends of the deep hole plate, a fixed shaft is fixedly connected between the pair of rectangular blocks, a pair of cover plates are placed in close contact with the upper end of the deep hole plate, a plurality of nozzles are fixedly connected to the lower end of the cover plates, a rubber plate is movably inserted into the cover plate, a push-pull rod is fixedly connected to the upper end of the rubber plate, an L-shaped block is fixedly connected to the end of the pair of deep hole plates away from each other, a sleeve ring is fixedly connected to the lower end of the L-shaped block, and a pair of pull-out plates with a plurality of liquid receiving grooves are movably inserted into the liquid receiving tank.

[0007] In the above-mentioned protein high throughput purification device, it is convenient for personnel to clean the inside.

[0008] As a further improvement of the present application, the sleeve ring is rotatably sleeved on the fixed shaft, and in the initial state, the spray head is inserted into the deep groove.

[0009] As a further improvement of the present application, a pair of support blocks are fixedly connected to the pair of side ends of the liquid receiving tank, and the outer diameter of the spray head is smaller than the diameter of the deep groove.

[0010] As a further improvement of the present application, the number of deep grooves is equal to the number of liquid receiving grooves, and the positions of the deep grooves and the liquid receiving grooves in the vertical direction correspond one by one.

[0011] As a further improvement of the present application, the upper end of the cover plate is provided with a sliding groove, the sliding groove is slidably connected with a sliding block, the upper end of the sliding block is fixedly connected with a limiting tube, and the outer end of the push-pull rod is provided with a limiting groove movably inserted with the limiting tube.

[0012] As a further improvement of the present application, a pair of side ends of the sliding block are coated with a magnetic coating one, and a pair of inner side walls of the sliding groove are coated with a magnetic coating two.

[0013] In summary, when the present application is used, liquid is injected into the deep groove, the gasket therein is used to absorb and filter the liquid, the filtered liquid flows out of the deep hole plate and falls into the liquid receiving groove, then the pull-out plate is replaced regularly to prevent the liquid from overflowing from the liquid receiving groove, after purification processing, the cover plate is turned down, the spray head enters the deep groove, the push-pull rod is pressed, water is sprayed out of the spray head under the pressure of the rubber plate, and the inner wall of the deep groove is washed. Therefore, when the present application is used, the cleaning steps are simple and time-saving, and the personnel operation is convenient. After cleaning, clean water is sucked into the cover plate for storage for next time cleaning and use, and the deep groove is sealed by the cover plate to prevent dust from polluting the inside. BRIEF DESCRIPTION OF DRAWINGS

[0014] Figure 1 It is a schematic diagram of the overall structure of the first and second embodiments of the present application;

[0015] Figure 2 It is a schematic diagram of the overall structure of the first and second embodiments of the present application; Figure 1 It is a partial enlarged schematic diagram of A in the present application;

[0016] Figure 3 It is a rotating schematic diagram of the cover plate of the first embodiment of the present application;

[0017] Figure 4 It is a partial enlarged schematic diagram of B in the present application; Figure 3

[0018] It is a schematic diagram of the overall structure of the first and second embodiments of the present application; Figure 5 ​

[0019] Figure 6 The schematic diagram of the sleeve ring of the first embodiment of the application is shown in the figure;

[0020] Figure 7 The schematic diagram of the rubber plate of the first embodiment of the application is shown in the figure

[0021] Figure 8 The schematic diagram of the cover plate of the first embodiment of the application is shown in the figure;

[0022] Figure 9 The schematic diagram of the cover plate of the first embodiment of the application is shown in the figure;

[0023] Figure 10 The schematic diagram of the cover plate of the first embodiment of the application is shown in the figure; Figure 8 The schematic diagram of the cover plate of the first embodiment of the application is shown in the figure;

[0024] Explanation of the figure:

[0025] 1, liquid receiving tank; 2, deep hole plate; 3, water outlet hole; 4, gasket; 5, rectangular block; 6, cover plate; 7, spray head; 8, rubber plate; 9, push-pull rod; 10, L block; 11, sleeve ring; 12, pull-out plate; 13, deep groove; 14, support block; 15, liquid receiving groove; 16, sliding groove; 17, sliding block; 18, limiting tube; 19, limiting groove; 20, magnetic coating I; 21, magnetic coating II. DETAILED DESCRIPTION

[0026] The two embodiments of the application will be described in detail below with reference to the accompanying drawings.

[0027] First embodiment:

[0028] Figures 1-9 A protein high-throughput purification device is shown, which comprises a liquid receiving tank 1, the upper end of the liquid receiving tank 1 is sleeved with a deep hole plate 2, the upper end of the deep hole plate 2 is provided with a plurality of deep grooves 13, the inner bottom wall of the deep grooves 13 is provided with water outlet holes 3 that are in communication with the liquid receiving tank 1, a gasket 4 is inserted into the inside of the deep hole plate 2, a pair of rectangular blocks 5 are fixedly connected to one pair of side ends of the deep hole plate 2 respectively, a fixed shaft is fixedly connected between the pair of rectangular blocks 5, a pair of cover plates 6 are placed in close contact with the upper end of the deep hole plate 2, the inside of the cover plate 6 is a hollow structure, a plurality of spray heads 7 are fixedly connected in communication with the lower end of the cover plate 6, a rubber plate 8 is movably inserted into the inside of the cover plate 6, a push-pull rod 9 is fixedly connected to the upper end of the rubber plate 8, an L block 10 is fixedly connected to the end of the pair of deep hole plates 2 that are away from each other, a sleeve ring 11 is fixedly connected to the lower end of the L block 10, a pair of pull-out plates 12 with a plurality of liquid receiving grooves 15 are movably inserted into the inside of the liquid receiving tank 1, the material of the liquid receiving tank 1, the deep hole plate 2 and the pull-out plate 12 is transparent polyvinyl chloride;

[0029] The sleeve joint ring 11 is rotatably sleeved on the fixed shaft, in the initial state, the spray head 7 is inserted in the deep groove 13, a pair of support blocks 14 are fixedly connected on a pair of side ends of the liquid receiving tank 1 respectively, the outer diameter of the spray head 7 is smaller than the diameter of the deep groove 13, the number of the deep grooves 13 is equal to the number of the liquid receiving grooves 15, and the deep grooves 13 and the liquid receiving grooves 15 are one-to-one corresponding in the vertical direction.

[0030] The application is used in the following steps:

[0031] Step one: when used, turn the cover plate 6 by one hundred and eighty degrees, so that it is in the position of Figure 3 , then place the gasket 4 in the deep groove 13, then pour the liquid to be purified into different deep grooves 13 in turn, filter and adsorb the liquid by the gasket 4, purify the liquid, reduce the content of impurities in the liquid, then the liquid will pass through the gasket 4 into the liquid receiving groove 15, when a certain amount of liquid is observed in the liquid receiving groove 15, in order to prevent the liquid from overflowing from the liquid receiving groove 15, the purification operation can be temporarily stopped, the pull-out plate 12 after receiving the liquid is taken out of the liquid receiving tank 1, the liquid is received by the liquid receiving groove 15 on another pull-out plate 12, so that personnel can quickly replace the liquid receiving mechanism, improve the efficiency of purifying liquid, and finally continue the purification operation;

[0032] Step two: after purification, take out the pull-out plate 12 from the liquid receiving tank 1, then take out the liquid in the liquid receiving groove 15, clean the pull-out plate 12 and the liquid receiving groove 15, then take off the deep hole plate 2 from the liquid receiving tank 1, pour out the gasket 4 and impurities in the deep groove 13, then again, the deep hole plate 2 is sleeved on the liquid receiving tank 1, rotate a pair of cover plates 6, and move them to Figure 1 the position of Figure 1 (in the initial state of , a certain amount of water is stored in the cover plate 6), then press the push-pull rod 9 downward, so that the rubber plate 8 extrudes the water in the cover plate 6, under the pressure of the rubber plate 8, the water is sprayed out of the spray head 7, the inner wall of the deep groove 13 is washed, the impurities mixed with the water flow out of the deep groove 13 through the water outlet hole 3 and fall into the liquid receiving tank 1, finally the sewage is taken out of the liquid receiving tank 1, so that the cleaning of the inside of the deep groove 13 is completed, the cleaning speed is fast, and the cleaning operation steps are simple;

[0033]

[0033] Step three: after cleaning, take off the sleeve joint ring 11 from the fixed shaft (the material of the sleeve joint ring 11 is nitrile rubber, which has good elasticity), separate the cover plate 6 from the deep hole plate 2, then insert the spray head 7 into the water, pull the push-pull rod 9 upward, so that it drives the rubber plate 8 to move upward, absorbs and temporarily stores water in the cover plate 6 (its water storage principle is similar to that of a needle cylinder syringe), then again, the sleeve joint ring 11 is sleeved on the fixed shaft, so that the cover plate 6 contacts the deep hole plate 2 (combined with Figure 1The cover plate 6 is used to seal the deep groove 13, and the deep groove 13 is protected from dust and impurities.

[0034] In summary, in use, liquid is injected into the deep groove 13, and the gasket 4 therein is used to adsorb and filter the liquid. The filtered liquid flows out of the deep hole plate 2 and falls into the liquid receiving groove 15. Then, the pull plate 12 is replaced in a timely manner to prevent the liquid from overflowing from the liquid receiving groove 15. After purification processing, the cover plate 6 is turned downward, the spray head 7 enters the deep groove 13, and the push-pull rod 9 is pressed. Under the pressure of the rubber plate 8, water is sprayed from the spray head 7 to wash the inner wall of the deep groove 13. Therefore, in use, the cleaning steps are simple and time-saving, and the personnel operation is convenient. After cleaning, clean water is sucked into the cover plate 6 for storage for next time cleaning and use. The cover plate 6 is used to seal the deep groove 13, so that the inside is not easily contaminated by dust, and the deep groove 13 is protected from dust.

[0035] Second embodiment:

[0036] The embodiment adds the following structure on the basis of the first embodiment, and the remaining parts remain the same as the first embodiment, as follows:

[0037] Figure 1 With Figure 10 The upper end of the cover plate 6 is provided with a sliding groove 16, the sliding groove 16 is slidably connected with a sliding block 17, the upper end of the sliding block 17 is fixedly connected with a limiting tube 18, the outer end of the push-pull rod 9 is provided with a limiting groove 19 movably inserted with the limiting tube 18, a pair of side ends of the sliding block 17 are both coated with a magnetic coating one 20, a pair of inner side walls of the sliding groove 16 are both coated with a magnetic coating two 21, and the magnetic coating one 20 and the magnetic coating two 21 are adsorbed to improve the stability of the sliding block 17 in the sliding groove 16, and further improve the stability of the limiting tube 18 in the limiting groove 19.

[0038] After storing water in the cover plate 6, the sliding block 17 is pushed to drive the limiting tube 18 to move towards the push-pull rod 9 until one end of the limiting tube 18 is inserted into the limiting groove 19. The limiting tube 18 is used to limit the push-pull rod 9 to prevent the water in the cover plate 6 from being pushed out due to accidental touching of the push-pull rod 9.

[0039] In combination with the current actual demand, the above-mentioned embodiments of the present application are not limited to this, and various changes made within the knowledge range of those skilled in the art without departing from the concept of the present application still fall within the protection scope of the present application.

Claims

1. A protein high-throughput purification device comprising a liquid receiving tank (1), characterized in that: The upper end of the liquid receiving tank (1) is sleeved with a deep hole plate (2), a plurality of deep grooves (13) are formed in the upper end of the deep hole plate (2), a water outlet hole (3) penetrating through the liquid receiving tank (1) is formed in the inner bottom wall of the deep groove (13), a gasket (4) is inserted into the deep hole plate (2), a pair of rectangular blocks (5) are fixedly connected to the pair of side ends of the deep hole plate (2), a fixed shaft is fixedly connected between the pair of rectangular blocks (5), a pair of cover plates (6) are placed on the upper end of the deep hole plate (2), a plurality of spray heads (7) are fixedly connected to the lower end of the cover plate (6), a rubber plate (8) is movably inserted into the cover plate (6), a push-pull rod (9) is fixedly connected to the upper end of the rubber plate (8), L-shaped blocks (10) are fixedly connected to the ends of the pair of deep hole plates (2) away from each other, sleeve rings (11) are fixedly connected to the lower ends of the L-shaped blocks (10), and a pair of pull-out plates (12) provided with a plurality of liquid receiving grooves (15) are movably inserted into the liquid receiving tank (1).

2. The protein high-throughput purification device according to claim 1, characterized in that: The sleeve ring (11) is rotatably sleeved on the fixed shaft, and in the initial state, the spray head (7) is inserted into the deep groove (13).

3. The protein high-throughput purification device of claim 2, wherein: A pair of support blocks (14) are fixedly connected to the pair of side ends of the liquid receiving tank (1), and the outer diameter of the spray head (7) is smaller than the diameter of the deep groove (13).

4. The protein high throughput purification device of claim 3, wherein: The number of the deep grooves (13) is equal to the number of the liquid receiving grooves (15), and the deep grooves (13) and the liquid receiving grooves (15) correspond to each other in the vertical direction.

5. The protein high throughput purification device of claim 4, wherein: A sliding groove (16) is formed in the upper end of the cover plate (6), a sliding block (17) is slidably connected in the sliding groove (16), a limiting tube (18) is fixedly connected to the upper end of the sliding block (17), and a limiting groove (19) movably inserted with the limiting tube (18) is formed in the outer end of the push-pull rod (9).

6. The protein high throughput purification device of claim 5, wherein: A magnetic coating one (20) is coated on the pair of side ends of the sliding block (17), and a magnetic coating two (21) is coated on the pair of inner side walls of the sliding groove (16).

Images