DNA extracting and filtering device

Abstract

The utility model discloses a DNA (deoxyribonucleic acid) extracting and filtering device, which relates to the technical field of DNA extraction and comprises a filtering cylinder body, a first connecting part arranged at the bottom of the filtering cylinder body and used for being connected with a silicon column inner cylinder, a second connecting part arranged at the top of the filtering cylinder body and used for being connected with a centrifugal tube, and filtering holes arranged on the connecting parts, one end of the filter hole is communicated with the inner cavity of the filter cylinder, the other end of the filter hole is communicated with the silicon column inner cylinder, and the side wall of the filter cylinder is provided with an accommodating groove for accommodating a sealing cover of the silicon column inner cylinder; according to the utility model, the accommodating groove for accommodating the sealing cover of the silicon column is formed in the side wall of the filter cylinder body, so that when the silicon column inner cylinder is connected with the filter cylinder body, the sealing cover can directly enter the accommodating groove without being cut off or bent downwards, the operation steps are reduced, the operation time is saved, the operation convenience is improved, and the pollution risk is reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to DNA extraction technical field, in particular to a DNA extraction filter device. BACKGROUND

[0002] In genomics research, the extraction technology of DNA is the key to the smooth research, and the researchers of genomics have been committed to the improvement of DNA extraction technology, and the highest extraction efficiency known extraction method is to increase the DNA binding liquid to the multiple volume of DNA lysis liquid, and then use the purification kit for purification, and the silicon column (DNA silica gel model adsorption column) can only accommodate a small amount of mixed liquid at most once, in order to reduce the operation steps and avoid pollution, the main method known at present is to connect the inner cylinder of the silicon column with the silicon membrane to a larger capacity filter device, and then put the filter device into the centrifugal tube, and make the binding liquid pass through the filter device and enter the binding silicon column again through low speed centrifugation, so that the DNA is combined on the silicon membrane of the silicon column, but the filter device known at present is not designed for the silicon column, and the outer diameter of the filter device is generally matched with the inner diameter of the centrifugal tube, so that before the filter device is connected with the inner cylinder of the silicon column, the sealing cap of the silicon column for purification needs to be cut off or the connecting band with the sealing cap is bent downward, and the sealing cap is covered again after the filtration is completed and the filter device is removed, and the operation of cutting off the sealing cap and bending downward will greatly increase the risk of pollution.

[0003] Therefore, people urgently need a DNA extraction filter device with low pollution risk. SUMMARY

[0004] The utility model discloses a DNA extraction filter device, which solves the problems of the prior art, provides a space for the sealing cap of the silicon column with the accommodating groove, and reduces the risk of pollution.

[0005] To achieve the above object, the utility model provides the following scheme: the utility model provides a DNA extraction filter device, which comprises a filter cylinder, a first connecting part for connecting the inner cylinder of the silicon column is arranged at the bottom of the filter cylinder, a second connecting part for connecting with the centrifugal tube is arranged at the top of the filter cylinder, a filter hole is arranged on the connecting part, one end of the filter hole is communicated with the inner cavity of the filter cylinder, and the other end is communicated with the inner cylinder of the silicon column, and an accommodating groove for accommodating the sealing cap of the inner cylinder of the silicon column is arranged on the side wall of the filter cylinder.

[0006] Preferably, the first connecting part is snap-connected with the silicon column inner cylinder, the first connecting part comprises at least two arc-shaped connecting plates with elastic self-recovery capability, the arc-shaped connecting plates are arranged around the filtering hole, the concave side of the arc-shaped connecting plate is arranged towards the filtering hole, the adjacent arc-shaped connecting plates are arranged at intervals, and a strip-shaped protrusion for clamping an end annular flange of the silicon column inner cylinder is arranged on the end face of the arc-shaped connecting plate close to the filtering hole, the strip-shaped protrusions of the adjacent arc-shaped connecting plates are located on the same horizontal plane, and the interval area between the adjacent arc-shaped connecting plates corresponds to the accommodating groove.

[0007] Preferably, the first connecting part further comprises a plug-in sleeve, the plug-in sleeve is arranged on one side of the arc-shaped connecting plate close to the filtering hole, and the outer peripheral wall of the plug-in sleeve is plug-connected with the inner peripheral wall of the silicon column inner cylinder.

[0008] Preferably, an extension sleeve is arranged on one side of the filtering hole close to the silicon column inner cylinder, the inner diameter of the extension sleeve matches the hole diameter of the filtering hole, one end of the extension sleeve away from the filtering hole is located in the inner cavity of the silicon column inner cylinder, and the extension sleeve is located inside the plug-in sleeve.

[0009] Preferably, the arc-shaped connecting plate, the plug-in sleeve and the extension sleeve are integrally arranged with the filtering cylinder body.

[0010] Preferably, the second connecting part is a ring-shaped plate, the inner edge of the ring-shaped plate is connected with the filtering cylinder body, the outer edge of the ring-shaped plate has a diameter greater than the inner diameter of the tube opening of the centrifugal tube and not greater than the outer diameter of the tube opening of the centrifugal tube.

[0011] Preferably, the ring-shaped plate is integrally arranged with the filtering cylinder body.

[0012] Preferably, the bottom of the filtering cylinder body is in a conical frustum type with a gradually decreasing diameter.

[0013] Preferably, the outer diameter of the filtering cylinder body matches the inner diameter of the centrifugal tube.

[0014] Preferably, the material of the filtering cylinder body is polypropylene.

[0015] Compared with the prior art, the utility model mainly obtains the following technical effects:

[0016] The accommodating groove for accommodating the sealing cover of the silicon column is arranged on the side wall of the filtering cylinder body, so that when the silicon column inner cylinder is connected with the filtering cylinder body, the sealing cover can directly enter the accommodating groove, without needing to be cut off or bent downward, thereby reducing operation steps, saving operation time, improving operation convenience and reducing the risk of pollution.

[0017] Compared with the prior art, the other scheme of the utility model obtains the following technical effects:

[0018] The arc-shaped connecting plate and the strip-shaped protrusion can improve the stability of the connection between the inner cylinder of the silicon column and the filter cylinder.

[0019] The friction between the insert tube and the silicon column, combined with the arc-shaped connecting plate, can further improve the stability of the connection between the inner cylinder of the silicon column and the filter body. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0021] Fig. 1 This is a schematic diagram of the DNA extraction and filtration device in an embodiment of the present invention;

[0022] Fig. 2 This is a schematic diagram of the structure of one side of the receiving tank of the DNA extraction and filtration device in an embodiment of this utility model;

[0023] Fig. 3 This is a schematic diagram of the bottom structure of the DNA extraction and filtration device in an embodiment of this utility model;

[0024] Fig. 4 This is a schematic diagram of the structure of the DNA extraction and filtration device, centrifuge tube, and silicon column inner cylinder connected in an embodiment of this utility model;

[0025] Fig. 5 for Fig. 4 Enlarged view of the structure at point A in the middle;

[0026] The components include: 1. Filter cylinder; 2. Receiving tank; 3. Arc-shaped connecting plate; 4. Strip-shaped protrusion; 5. Insertion cylinder; 6. Extension cylinder; 7. Annular plate; 8. Silicon column inner cylinder; 9. Connecting strip; 10. Sealing cap; 11. Centrifuge tube. Detailed Implementation

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0028] The utility model discloses a DNA extraction filter device to solve the prior art problems, and the sealing cover of the silicon column is provided with containing space by containing groove, and the pollution risk is reduced.

[0029] In order to make the above-mentioned purpose, features and advantages of the utility model more obvious and easy to understand, the utility model is further explained in detail below by combining with the drawings and specific embodiments.

[0030] Please refer to as Figs. 1-5 As shown in the figure, a DNA extraction filter device is provided, which comprises a filter cylinder body 1, the bottom of the filter cylinder body 1 is provided with a first connecting part, the top of the filter cylinder body 1 is provided with a second connecting part, the first connecting part is used for connecting the silicon column inner cylinder 8, the second connecting part is used for connecting with the centrifugal tube 11, the connecting part is provided with a filter hole, one end of the filter hole is communicated with the inner cavity of the filter cylinder body 1, and the other end is communicated with the silicon column inner cylinder 8; in the embodiment, the filter hole is a straight-through hole, and the pore size of the filter hole is not greater than 1mm; the sidewall of the filter cylinder body 1 is provided with a containing groove 2, the containing groove 2 is used for containing the sealing cover 10 of the silicon column inner cylinder 8, and the containing groove 2 is formed by the inward recess of the outer wall of the filter cylinder body 1; in the device, the setting of the containing groove 2 makes the sealing cover 10 directly enter the containing groove 2 when the silicon column inner cylinder 8 is connected with the filter cylinder body 1, so that the sealing cover 10 does not need to be cut or bent downward, the operation steps are reduced, the operation time is saved, the operation convenience is improved, and the pollution risk is reduced.

[0031] In the embodiment, the first connecting part is snap-connected with the silicon column inner cylinder 8, which is convenient for installation and dismounting, can ensure the stability of the connection between the silicon column inner cylinder 8 and the filter cylinder body 1, and can prevent the silicon column inner cylinder 8 from being separated from the filter cylinder body 1 during centrifugation; the specific structure of the snap connection is as follows: the first connecting part comprises at least two arc-shaped connecting plates 3 with elastic self-recovery capability, the arc-shaped connecting plates 3 are arranged around the filter hole, preferably, all the arc-shaped connecting plates 3 are uniformly arranged around the filter hole, the concave side of the arc-shaped connecting plate 3 is arranged to face the filter hole, the adjacent arc-shaped connecting plates 3 are arranged at intervals, the end face of the arc-shaped connecting plate 3 close to the filter hole is provided with a strip-shaped protrusion 4, the strip-shaped protrusion 4 is used for clamping the end part annular flange of the silicon column inner cylinder 8, the strip-shaped protrusions 4 of the adjacent arc-shaped connecting plates 3 are located on the same horizontal plane, and the interval region between the adjacent arc-shaped connecting plates 3 is provided with the containing groove 2, so as to provide a placing space for the connecting band 9 between the silicon column inner cylinder 8 and the sealing cover 10; in other embodiments, interference insertion and other connection modes can also be selected.

[0032] The specific principle of the first connecting part buckling connecting with the silicon column inner cylinder 8 in the embodiment is as follows: after the silicon column inner cylinder 8 enters the inner side of all the arc-shaped connecting plates 3, the end annular flange thereof first contacts the strip-shaped protrusion 4, at this time, the silicon column inner cylinder 8 continues to extrude the strip-shaped protrusion 4, so that all the arc-shaped connecting plates 3 are in the flared state, when the end of the silicon column inner cylinder 8 enters the space between the strip-shaped protrusion 4 and the filter cylinder body 1, the strip-shaped protrusion 4 is no longer extruded, the arc-shaped connecting plate 3 resets, and the strip-shaped protrusion 4 realizes the blocking of the annular flange of the end of the silicon column inner cylinder 8, thereby preventing it from falling out.

[0033] The cross section of the strip-shaped protrusion 4 is preferably semicircular.

[0034] The arc of the arc-shaped connecting plate 3 in the embodiment can match the outer edge of the annular flange of the end of the silicon column inner cylinder 8, or can not match, when matching, the strip-shaped protrusion 4 at any position can block the silicon column inner cylinder 8, when not matching, the arc-shaped connecting plate 3 only needs to ensure that the arc of the arc-shaped connecting plate 3 has contact with the outer edge arc of the annular flange of the end of the silicon column inner cylinder 8, at this time, part of the strip-shaped protrusion 4 will block the silicon column inner cylinder 8.

[0035] The first connecting part further comprises a plug-in cylinder 5, the plug-in cylinder 5 is arranged on one side of the arc-shaped connecting plate 3 close to the filter hole, the outer peripheral wall of the plug-in cylinder 5 is plugged with the inner peripheral wall of the silicon column inner cylinder 8, and the friction between the plug-in cylinder 5 and the silicon column cooperates with the arrangement of the arc-shaped connecting plate 3, so that the stability of the connection between the silicon column inner cylinder 8 and the filter cylinder body 1 can be further improved.

[0036] An extension cylinder 6 is arranged on the side of the filter hole close to the silicon column inner cylinder 8, the inner diameter of the extension cylinder 6 matches the hole diameter of the filter hole, the end of the extension cylinder 6 away from the filter hole is located in the inner cavity of the silicon column inner cylinder 8, the extension cylinder 6 is located on the inner side of the plug-in cylinder 5, the extension cylinder 6 guides the filtered liquid and directly introduces it into the silicon column inner cylinder 8, when the extension cylinder 6 is arranged, the inner cavity of the extension cylinder 6 serves as an extension of the filter hole.

[0037] The arc-shaped connecting plate 3, the plug-in cylinder 5 and the extension cylinder 6 are integrally arranged with the filter cylinder body 1, so that the structural strength is improved.

[0038] The second connecting part is a ring-shaped plate 7, the inner edge of the ring-shaped plate 7 is connected with the filter cylinder body 1, the outer edge of the ring-shaped plate 7 has a diameter greater than the inner diameter of the pipe opening of the centrifugal tube 11 and less than the outer diameter of the pipe opening of the centrifugal tube 11, so that the ring-shaped plate 7 can be lapped on the pipe opening of the centrifugal tube 11 during installation, after the cover body of the centrifugal tube 11 is screwed and connected, the cover body cooperates with the pipe opening to limit the ring-shaped plate 7, thereby limiting the filter cylinder body 1; in other embodiments, the second connecting part can be a plurality of block structures, the block structures are uniformly arranged around the end of the filter cylinder body 1, the block structures are used for lapping on the pipe opening of the centrifugal tube 11, and the plurality of block structures form a ring-shaped structure matched with the ring-shaped plate 7.

[0039] The annular plate 7 is integrally arranged with the filter cylinder 1, and the structural strength is improved.

[0040] In order to reduce liquid residues in the filter device, the bottom of the filter cylinder 1 is in the form of a frustum with gradually reduced diameter.

[0041] The outer diameter of the filter cylinder 1 matches the inner diameter of the centrifugal tube 11, and the stability during centrifugation is improved.

[0042] The material of the filter cylinder 1, the first connecting part and the second connecting part is plastic, preferably high-purity polypropylene, which reduces the binding force between the tube wall and the DNA sample and ensures the recovery rate of the DNA by the silica column inner tube 8.

[0043] In actual use, the inner cavity of the filter cylinder 1 should have a corresponding air inlet passage. The air inlet passage can be a hole in the centrifugal tube 11, or a centrifugal tube 11 with a trace gap for gas to enter after the cover is threadedly connected.

[0044] According to actual needs, adaptive changes are all within the protection scope of the utility model.

[0045] It should be noted that, for those skilled in the art, it is obvious that the utility model is not limited to the details of the above exemplary embodiments, and the utility model can be realized in other specific forms without departing from the spirit or basic characteristics of the utility model. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting, and the scope of the utility model is defined by the appended claims rather than the above description, and therefore all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the utility model. Any reference signs in the claims should not be regarded as limiting the claims involved.

[0046] The principle and implementation mode of the utility model are described by applying specific examples in the utility model, and the above embodiment description is only used to help understand the method and core idea of the utility model; at the same time, for those skilled in the art, according to the idea of the utility model, the specific implementation mode and application range will be changed. In conclusion, the content of the specification should not be understood as a limitation of the utility model.

Claims

1. A DNA extraction filter device, characterized by, The filter cartridge comprises a filter cartridge body, a first connecting part at the bottom of the filter cartridge body for connecting a silica column inner cylinder, a second connecting part at the top of the filter cartridge body for connecting a centrifugal tube, a filter hole provided on the connecting part, one end of the filter hole being in communication with the inner cavity of the filter cartridge body, and the other end being in communication with the silica column inner cylinder, and a containing groove provided on the side wall of the filter cartridge body for accommodating a sealing cover of the silica column inner cylinder.

2. The DNA extraction filter device according to claim 1, wherein, The first connecting part is snap-connected with the silica column inner cylinder, the first connecting part comprises at least two arc-shaped connecting plates with elastic self-recovery capability, the arc-shaped connecting plates are arranged around the filter hole, the concave side of the arc-shaped connecting plates is arranged towards the filter hole, the adjacent arc-shaped connecting plates are arranged at intervals, the end surface of the arc-shaped connecting plates close to the filter hole is provided with a strip-shaped protrusion for clamping the end annular flange of the silica column inner cylinder, the strip-shaped protrusions of the adjacent arc-shaped connecting plates are located on the same horizontal plane, and the interval area between the adjacent arc-shaped connecting plates corresponds to the containing groove.

3. The DNA extraction filter device according to claim 2, wherein, The first connecting part further comprises a plug-in cylinder, the plug-in cylinder is arranged on the side of the arc-shaped connecting plate close to the filter hole, and the outer peripheral wall of the plug-in cylinder is plug-connected with the inner peripheral wall of the silica column inner cylinder.

4. The DNA extraction filter device according to claim 3, wherein, The filter hole is provided with an extension cylinder on the side close to the silica column inner cylinder, the inner diameter of the extension cylinder matches the hole diameter of the filter hole, the end of the extension cylinder away from the filter hole is located in the inner cavity of the silica column inner cylinder, and the extension cylinder is located inside the plug-in cylinder.

5. The DNA extraction filter device according to claim 4, wherein, The arc-shaped connecting plate, the plug-in cylinder and the extension cylinder are integrally arranged with the filter cartridge body.

6. The DNA extraction filter device according to claim 1, wherein, The second connecting part is a ring-shaped plate, the inner edge of the ring-shaped plate is connected with the filter cartridge body, the outer edge of the ring-shaped plate has a diameter greater than the inner diameter of the tube opening of the centrifugal tube and not greater than the outer diameter of the tube opening of the centrifugal tube.

7. The DNA extraction filter device according to claim 6, wherein, The ring-shaped plate is integrally arranged with the filter cartridge body.

8. The DNA extraction filter device of claim 1, wherein, The bottom of the filter cartridge body is in the form of a conical frustum with gradually decreasing diameter.

9. The DNA extraction filter device according to claim 1, wherein, The outer diameter of the filter cartridge body matches the inner diameter of the centrifugal tube.

10. The DNA extraction filter device of claim 1, wherein, The material of the filter cartridge body is polypropylene.

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