Protein precipitation filtering device

Abstract

The utility model discloses a protein precipitating and filtering device, which relates to the technical field of precipitating and filtering and comprises a precipitating and filtering plate, a collecting plate and a filter pressing component. A plurality of sample tubes are arrayed on the precipitation filter plate, and the bottoms of the sample tubes are opened and are provided with filter plates; a plurality of collecting pipes are arrayed on the collecting plate, and when the precipitation filtering plate is assembled above the collecting plate, the collecting pipes correspond to the sample pipes in position and are used for collecting liquid flowing out of the sample pipes; the filter pressing assembly comprises a flat plate and a plurality of piston rods arranged at the bottom of the flat plate, plungers are arranged at the bottoms of the piston rods, and the positions and sizes of the plungers are matched with corresponding sample tubes. According to the scheme, the problem that a protein precipitation process and a filtering process cannot be performed simultaneously is solved; a large number of samples can be processed at a time, and the sample pretreatment efficiency is improved; the operation is simple and fast, and the experiment waiting time is shortened.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of sedimentation filtration, especially relates to a protein sedimentation filtration device. BACKGROUND

[0002] The existing protein removal technology is usually adding a certain amount of protein precipitant in the EP tube preloaded with samples, mixing, and then taking supernatant by centrifugation or taking filtrate by filtration. The existing method has the following disadvantages: only applicable to the treatment of a small amount of samples, and repeated operation is more and operation is complex when treating a large amount of samples, and it takes a long time. UTILITY MODEL CONTENT

[0003] To solve the problems in the prior art, the utility model provides a protein sedimentation filtration device which can remove the proteins of multiple samples at the same time.

[0004] In order to achieve the purpose of the utility model, the following scheme is adopted:

[0005] A protein sedimentation filtration device comprises a sedimentation filtration plate, a collection plate and a filter pressing assembly.

[0006] The array of sedimentation filtration plates has multiple sample tubes, the bottom of each sample tube is open, and each sample tube is provided with a filtration plate;

[0007] The array of collection plates has multiple collection tubes, the collection tubes correspond to the positions of the sample tubes when the array of sedimentation filtration plates is assembled above the array of collection plates, and the collection tubes are used to collect the liquid flowing out of the sample tubes;

[0008] The filter pressing assembly comprises a flat plate and multiple piston rods arranged at the bottom of the flat plate, the bottom of each piston rod is provided with a plunger, and the position and size of the plunger are used to match the corresponding sample tube.

[0009] Further, the filtration plate is pressed from polytetrafluoroethylene particles with a diameter of 0.2 microns and a thickness of 1 mm, and a filter sheet made of polyethylene is attached to each side of the filtration plate, and the pore size of the filter sheet is 0.22 microns.

[0010] Further, the sample tube has a diameter of 8 mm and a height of 40 mm, the bottom of the sample tube is provided with a transition tube with a height of 10 mm, and the inner diameter of the transition tube gradually decreases to 1 mm from top to bottom.

[0011] Further, the plunger is made of acrylic rubber.

[0012] Further, the number of sample tubes, collection tubes and plungers is 96.

[0013] Further, the device is made of transparent material except the plungers.

[0014] Further, the flat plate is provided with handles at both ends.

[0015] Further, the outer periphery of the bottom of the sedimentation filter plate is provided with a first rectangular shell, the outer periphery of the bottom of the collection plate is provided with a second rectangular shell, the bottom surface of the second rectangular shell is lower than the bottom surface of the collection tube, the top of the second rectangular shell is provided with a stepped clamping groove, and the first rectangular shell is matched with the stepped clamping groove.

[0016] Further, the side walls at both ends of the first rectangular shell and the second rectangular shell are provided with through grooves.

[0017] The protein sedimentation and filtration device has the advantages that:

[0018] 1. The protein sedimentation technology and the filtration technology are combined, and the problem that the protein sedimentation process and the filtration process cannot be simultaneously performed is solved.

[0019] 2. A large number of samples can be simultaneously processed at one time, and the sample pretreatment efficiency is increased.

[0020] 3. The operation is simple and fast, and the experimental waiting time is reduced. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 An explosion structure diagram of the protein sedimentation and filtration device is shown;

[0022] Figure 2 A structure diagram of the sedimentation filter plate is shown;

[0023] Figure 3 A sample tube perspective view is shown;

[0024] Figure 4 A collection plate structure diagram is shown;

[0025] Figure 5 A structure diagram of the protein sedimentation and filtration device after assembly is shown. DETAILED DESCRIPTION

[0026] As shown in the drawings, Figure 1 the embodiment provides a protein sedimentation and filtration device, which comprises a sedimentation filter plate 1, a collection plate 2 and a filter pressing assembly 3.

[0027] Specifically, as shown in the drawings, Figure 2 , Figure 3 the sedimentation filter plate 1 is arrayed with 96 sample tubes 11 and is distributed in 8 rows and 12 columns, the bottom of the sample tube 11 is provided with an opening and is provided with a filter plate 12, the tube diameter of the sample tube 11 is about 8 mm, the height is about 40 mm, the filter plate 12 is pressed from polytetrafluoroethylene particles with a diameter of about 0.2 μm, the thickness is about 1 mm, and a filter sheet made of polyethylene is attached to each of the two surfaces of the filter plate 12, and the pore size of the filter sheet is about 0.22 μm.

[0028] Specifically, as shown in the drawings, Figure 1 , Figure 2 , Figure 4As shown, the array of collection plates 2 has 96 collection tubes 21, which are arranged in 8 rows × 12 columns. When the sedimentation filter plate 1 is assembled above the collection plate 2, the positions of the collection tubes 21 and the sample tubes 11 correspond one-to-one, and are used to collect the liquid flowing out of the sample tubes 11.

[0029] To ensure that the filtered liquid in sample tube 11 flows smoothly into collection tube 21, such as Figure 2 , Figure 3 As shown, a transition tube with a height of about 10 mm is provided at the bottom of the sample tube 11. The inner diameter of the transition tube gradually decreases to about 1 mm from top to bottom. When the sedimentation filter plate 1 is assembled above the collection plate 2, the transition tube is inserted into the opening of the corresponding collection tube 21.

[0030] Regarding the assembly method of the sedimentation filter plate 1 and the collection plate 2, there are many existing implementation methods. This embodiment adopts the following method: Figure 1 , Figure 5 As shown, a first rectangular shell 13 is provided on the outer periphery of the bottom of the sedimentation filter plate 1, and a second rectangular shell 22 is provided on the outer periphery of the bottom of the collection plate 2. A stepped groove 23 is provided on the top of the second rectangular shell 22. The first rectangular shell 13 matches the stepped groove 23, as shown. Figure 5 The diagram shown is of the assembled structure.

[0031] To further improve the stability of the collecting plate 2, the bottom surface of the second rectangular shell 22 is lower than the bottom surface of the collecting tube 21.

[0032] To facilitate the operation of sedimentation filter plate 1 and collection plate 2, such as Figure 1 As shown, the side walls at both ends of the first rectangular shell 13 and the second rectangular shell 22 are provided with through slots 14.

[0033] Specifically, such as Figure 1 As shown, the filter press assembly 3 includes a plate 31 and 96 piston rods 32 located at its bottom. The 96 piston rods 32 are arranged in 8 rows × 12 columns. Each piston rod 32 has a plunger 33 at its bottom. The plunger 33 is made of acrylic rubber. The position and size of the plunger 33 are used to match the corresponding sample tube 11 and are well sealed.

[0034] For ease of operation of filter press assembly 3, such as Figure 1 As shown, the flat plate 31 has handles 34 at both ends.

[0035] To facilitate observation of the liquid filtration and collection process, all parts of the device, except for plunger 33, are made of transparent material.

[0036] In use, the 96-hole sedimentation filter plate 1 is placed on the 96-hole collection plate 2, and the two plates are just clamped; a proper amount of protein precipitant is added in advance into the 96-hole sedimentation filter plate 1, and then the sample to be treated is added and mixed thoroughly; the plunger 33 is inserted, and the flat plate 31 is pressed down, so that the filtered liquid flows into the corresponding collection tube 21. The embodiment can simultaneously process a large number of samples at a time, improves the efficiency, and solves the problem that the protein precipitation process and the filtration process cannot be simultaneously performed.

[0037] The above embodiments are only used for illustrating the technical ideas and characteristics of the present application, and do not represent the only or limit the present application. Those skilled in the art should understand that various changes or equivalent replacements made to the present application without departing from the scope of the present application all belong to the protection scope of the present application.

Claims

1. A protein precipitation filtration device, characterized by, The application relates to a device for separating and collecting liquid from a sample, comprising: a sedimentation and filtration plate (1) having an array of sample tubes (11) arranged with open bottoms and provided with a filtration plate (12); a collection plate (2) having an array of collection tubes (21) arranged to correspond to the sample tubes (11) when the sedimentation and filtration plate (1) is assembled above the collection plate (2) and used to collect liquid from the sample tubes (11); a filter pressing assembly (3) comprising a flat plate (31) and a plurality of piston rods (32) arranged at the bottom of the flat plate (31), wherein the bottom of each piston rod (32) is provided with a plunger (33) arranged to match the corresponding sample tube (11) in position and size.

2. The protein precipitation filtration device of claim 1, wherein, The filtration plate (12) is made of polytetrafluoroethylene particles with a diameter of 0.2 microns and a thickness of 1 mm, and a polyethylene filter sheet with a pore size of 0.22 microns is attached to each side of the filtration plate (12).

3. The protein precipitation filtration device of claim 1, wherein, The sample tube (11) has a tube diameter of 8 mm and a height of 40 mm, and the bottom of the sample tube (11) is provided with a transition tube with a height of 10 mm, wherein the inner diameter of the transition tube gradually decreases from top to bottom to 1 mm.

4. The protein precipitation filtration device of claim 1, wherein, The material of the plunger (33) is selected from acrylate rubber.

5. The protein precipitation filtration device of claim 1, wherein, The number of sample tubes (11), collection tubes (21) and plungers (33) is 96.

6. The protein precipitation filtration device of claim 1, wherein, Except for the plunger (33), the device is made of transparent material.

7. The protein precipitation filtration device of claim 1, wherein, The flat plate (31) is provided with handles (34) at both ends.

8. The protein precipitation filtration device of claim 1, wherein, The outer periphery of the bottom of the sedimentation and filtration plate (1) is provided with a first rectangular shell (13), and the outer periphery of the bottom of the collection plate (2) is provided with a second rectangular shell (22), wherein the bottom surface of the second rectangular shell (22) is lower than the bottom surface of the collection tube (21), the top of the second rectangular shell (22) is provided with a stepped clamping groove (23), and the first rectangular shell (13) matches the stepped clamping groove (23).

9. The protein precipitation filtration device of claim 8, wherein, The side walls of both ends of the first rectangular shell (13) and the second rectangular shell (22) are provided with through grooves (14).

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