Card type micro-fluidic chip for multi-target detection

Abstract

The utility model relates to a card type micro-fluidic chip for multi-target detection, which is characterized in that the lower part of a chip base body of the chip is provided with a wavy main flow channel along the width direction of the chip base body, one end of the main flow channel is connected with a sample adding flow channel along the length direction of the chip base body, and the end part of the sample adding flow channel is provided with a sample adding hole; each trough of the main flow channel is connected with a branch flow channel along the length direction of the chip substrate, and the outer end of the straight flow channel is connected with a reaction tank; according to the micro-fluidic chip, the wavy main flow channel with liquid storage capacity is designed, and during centrifugation, the storage capacity is beneficial for a sample to uniformly enter each branch flow channel from the main flow channel and then timely and uniformly enter each reaction tank, so that the time difference of the sample entering the reaction tanks is reduced, and the consistency of the reaction time of the sample and a reagent in each reaction tank is improved; and the simultaneous detection precision of multiple targets can be improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to micro -fluidic chip field especially, it relates to a multi -target detection's card type micro -fluidic chip. BACKGROUND

[0002] Micro -fluidic chip is a chip based, combines chemistry, physics, biology etc. Multidisciplinary technology platform formed, can realize sample preparation, reaction, separation, detection etc. Flow process on a chip, has high throughput, high efficiency and easy operation's characteristics. With the development of material science and nanotechnology, this technology gets rapid development and is applied to chemical analysis, microorganism detection etc., especially in microorganism detection and analysis plays an important role, and gradually produces commercialized micro -fluidic chip. Micro -fluidic chip because has miniaturization, saves reagent, fast, integrated degree is high advantage, has extensive application prospect in biology, chemistry, medicine etc. Micro -fluidic chip can carry out one or continuous multiple reactions through micro -fluidic path, but the flow channel in the micro -fluidic chip is usually linear arrangement, there is the problem that the sample in the flow channel flows too fast when centrifuging, leading to not timely and evenly entering each reaction pool, leading to the sample in each reaction pool and reagent reaction time is not one, influence multiple target simultaneous detection precision, need to be improved. SUMMARY

[0003] In order to solve the above problem, the utility model provides a multi -target detection's card type micro -fluidic chip.

[0004] The technical scheme of the utility model is: a multi -target detection's card type micro -fluidic chip, its characterized in that: the chip base body lower part of the chip is provided with wave shape main flow channel along its width direction, one end of main flow channel is connected with sample adding flow channel along the length direction of chip base body, and the end of sample adding flow channel is provided with sample adding hole;Each wave trough of main flow channel is connected with branch flow channel along the length direction of chip base body, and the outer end of linear flow channel is connected with reaction pool, and the chip base body is mainly made of polymethyl methacrylate, polydimethylsiloxane, organic glass and other materials.

[0005] Preferably, the reaction pool is arranged closely in staggered, and the branch flow channel connected with the reaction pool includes long branch flow channel and short branch flow channel, the short branch flow channel is connected with the reaction pool close to the inner side, and the long branch flow channel is connected with the reaction pool close to the outer side.

[0006] Preferably, the middle part of long branch flow channel is provided with buffer flow channel A, and the middle part of short branch flow channel is provided with buffer flow channel B larger than buffer flow channel A in size.

[0007] Preferably, the buffer flow channel A and buffer flow channel B are wave shape flow channels.

[0008] Preferably, the other end of the main flow channel is provided with an exhaust flow channel, and the end of the exhaust flow channel is provided with an exhaust hole.

[0009] Preferably, the chip substrate comprises an upper substrate and a lower substrate, and the upper substrate and the lower substrate are bonded to form an integrated body, and the flow channel structure inside the chip substrate is formed by the combination of the upper substrate and the lower substrate.

[0010] Preferably, the front surface of the chip substrate is attached with a two-dimensional code label, and the back surface is attached with a sample information label.

[0011] The beneficial technical effects of the utility model are that the microfluidic chip is designed with a wave-shaped main flow channel having liquid storage capacity, which is beneficial to the uniform entry of samples from the main flow channel into each branch flow channel during centrifugation, and then the timely and uniform entry into each reaction pool, thereby reducing the time difference of entering the reaction pool and improving the consistency of the reaction time of samples and reagents in each reaction pool, which is beneficial to improving the precision of simultaneous detection of multiple targets. BRIEF DESCRIPTION OF DRAWINGS

[0012] Figure 1 is the front main view structural schematic diagram of the utility model;

[0013] Figure 2 is the back main view structural schematic diagram of the utility model;

[0014] Figure 3 is the three-dimensional structural schematic diagram of the utility model;

[0015] Figure 4 is the three-dimensional structural schematic diagram of the utility model in the separated state of the upper substrate and the lower substrate.

[0016] In the drawing, 1. chip substrate, 11. upper substrate, 12. lower substrate, 2. main flow channel, 21. sample adding flow channel, 22. sample adding hole, 23. exhaust flow channel, 24. exhaust hole, 3. long branch flow channel, 31. buffer flow channel A, 4. short branch flow channel, 41. buffer flow channel B, 5. reaction pool, 6. foolproof notch. DETAILED DESCRIPTION

[0017] Example one, referring to the attached Figures 1-4 A cassette-type microfluidic chip for multiple target detection, the lower part of the chip substrate 1 of the chip is provided with a wave-shaped main flow channel 2 along the width direction thereof, the chip substrate 1 is designed in the shape of a cuboid with one foolproof notch 6, one end of the main flow channel 2 is connected with a sample adding flow channel 21 along the length direction of the chip substrate 1, the end of the sample adding flow channel is provided with a sample adding hole 22, and a pipettor is used for sample adding; each wave trough of the main flow channel 2 is connected with a branch flow channel along the length direction of the chip substrate 1, the outer end of the straight flow channel is connected with a reaction pool 5, the number of the reaction pools 5 is designed to be at least two, which can be suitable for detecting multiple pathogens, and the sizes of the reaction pools 5 are the same.

[0018] The card type micro-fluidic chip can complete a series of complex reactions in biological, chemical, and immunological fields through simple and effective integration, and can detect multiple detection targets of a sample in one chip.

[0019] The other end of the main flow channel 2 is provided with an exhaust flow channel 23, and an exhaust hole 24 is arranged at the end of the exhaust flow channel. When adding the sample, the gas in the flow channel can flow out from the exhaust hole 24.

[0020] The chip substrate 1 includes an upper substrate 11 and a lower substrate 12. The flow channel structure inside the chip substrate 1 is formed by combining the upper substrate 11 and the lower substrate 12. When manufacturing, arc-shaped grooves are processed on the surfaces of the two substrates, and the flow channel structure is formed inside after the two substrates are bonded.

[0021] The front surface of the chip substrate 1 is attached with a two-dimensional code label. The two-dimensional code label is a reagent instruction hyperlink. After the code is scanned and opened, the main content is a chip instruction manual, which mainly includes production date, validity period, detection items, and use method.

[0022] The use method of the micro-fluidic chip is as follows:

[0023] The sample is added to the micro-fluidic chip through the sample adding hole 22. When the sample fills all the main flow channels 2

[0024] , the adding of the sample is stopped;

[0025] ②Seal the sample adding hole 22 and the exhaust hole 24;

[0026] ③High-speed centrifugation;

[0027] ④Transfer the liquid in the main flow channel 2 to each reaction pool 5;

[0028] ⑤Check the sealing condition of the sample adding hole 22 and the exhaust hole 24;

[0029] ⑥Under the assistance of the matching equipment, the liquid reacts with the reagent pre-embedded in the reaction pool 5;

[0030]

[0031] ⑦Detect and analyze the reaction result.

[0032] The embodiment is mainly applied in clinical examination.

[0033] After the extracted DNA is mixed with the reaction solution, it is injected into the card type micro-fluidic chip

[0034] ​In the chip, the specific primers are embedded in the reaction pool 5 respectively; the above chip is centrifuged at high speed, 6000 rpm for 2 min; the constant temperature amplification reaction is carried out in the reaction pool 5 by heating at 63 DEG C for 45 min through the matching equipment, and finally the fluorescence in the reaction pool 5 is detected in real time by using the matching instrument to obtain the detection result.

[0035] The cassette microfluidic chip can be detected by fluorescence, turbidity, color development and instrument detection, and real-time detection during the reaction can be performed when necessary.

[0036] Embodiment two, see attached Figures 1-3 The embodiment is basically the same as embodiment one, and the same parts will not be repeated, and the different parts are that the reaction pool 5 is arranged staggered and closely, the branch flow channel connected with the reaction pool 5 includes the long branch flow channel 3 and the short branch flow channel 4, the short branch flow channel 4 is connected with the reaction pool 5 close to the inner side, the long branch flow channel 3 is connected with the reaction pool 5 close to the outer side, the arrangement of the reaction pool 5 is more compact, which is beneficial to save space size, and more reaction pools 5 can be arranged in the chip substrate 1 with the same size.

[0037] The middle part of the long branch flow channel 3 is provided with a buffer flow channel A 31, and the middle part of the short branch flow channel 4 is provided with a buffer flow channel B 41 with a size larger than the buffer flow channel A 31, the buffer flow channel A 31 and the buffer flow channel B 41 are both wave-shaped flow channels, the wave-shaped structure can generate flow resistance to the liquid, and play the role of liquid buffer flow, different sizes of buffer flow channels provide buffer flow for branch flow channels with different lengths, and the travel of the sample flowing into the reaction pool 5 is adjusted to be consistent through different buffer flow amplitudes, the sample can enter into each reaction pool 5 at the same time during centrifugation, the consistency of the reaction time of the sample and the reagent in each reaction pool 5 is improved, and the precision of simultaneous detection of multiple targets is improved.

Claims

1. A cassette microfluidic chip for multi-target detection, characterized in that: The chip base lower part of the chip is provided with a wave-shaped main flow channel along the width direction thereof, one end of the main flow channel is connected with a sample adding flow channel along the length direction of the chip base, and the end of the sample adding flow channel is provided with a sample adding hole; each wave trough of the main flow channel is connected with a branch flow channel along the length direction of the chip base, and the outer end of the branch flow channel is connected with a reaction pool.

2. The cassette microfluidic chip for multi-target detection according to claim 1, characterized in that: The reaction pools are closely arranged in an interlaced manner, the branch flow channels connected with the reaction pools include long branch flow channels and short branch flow channels, the short branch flow channels are connected with the reaction pools close to the inner side, and the long branch flow channels are connected with the reaction pools close to the outer side.

3. The cassette microfluidic chip for multi-target detection according to claim 2, characterized in that: The middle part of the long branch flow channel is provided with a buffer flow channel A, and the middle part of the short branch flow channel is provided with a buffer flow channel B which is larger in size than the buffer flow channel A.

4. The cassette microfluidic chip for multi-target detection according to claim 3, characterized in that: The buffer flow channels A and B are both wave-shaped flow channels.

5. The cassette microfluidic chip for multi-target detection according to claim 1, characterized in that: The other end of the main flow channel is provided with an exhaust flow channel, and the end of the exhaust flow channel is provided with an exhaust hole.

6. The cassette microfluidic chip for multi-target detection according to claim 1, characterized in that: The chip base comprises an upper base and a lower base, and the flow channel structure inside the chip base is formed by combination of the upper base and the lower base.

7. The cassette microfluidic chip for multi-target detection according to claim 1, characterized in that: The front surface of the chip base is attached with a two-dimensional code label, and the back surface is attached with a sample information label.

Images