Universal protein antibody purification column

Abstract

The utility model discloses a universal protein antibody purification column which comprises a hollow column body, a bottom column is detachably arranged at one end of the hollow column body, a discharge pipe is arranged at one end, far away from the hollow column body, of the bottom column, a funnel-shaped through opening is formed in the inner bottom wall of the bottom column, and the through opening is communicated with the discharge pipe. According to the technical scheme, a traditional integrated purification column is designed to be of a sectional structure, namely, the purification column comprises the hollow column body and the bottom column, when the lower gasket is placed, the bottom column and the hollow column body can be detached firstly, so that the length of the bottom column is reduced, an operator can put the lower gasket into the bottom column more conveniently, and after the lower gasket is well placed, the bottom column is separated from the hollow column body. The bottom column and the hollow column body can be connected again and again rapidly, the problem that when a lower gasket is placed on a traditional purification column, due to the fact that the diameter of the hollow column is small and the hollow column is deep and long, the step of placing the lower gasket is troublesome is effectively solved, in addition, a funnel-shaped through opening is formed in the inner bottom wall of the bottom column, and the through opening is communicated with a discharging pipe. And leaked substances can be quickly discharged through the discharge pipe.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the technical field of biomedical research, especially relate to a general protein antibody purification column. BACKGROUND

[0002] Protein antibody purification is a key technology in biomedical research and industrial production, aiming at separating high-purity antibodies from samples containing antibodies (such as serum, ascites, cell culture supernatant, etc.), usually referring to immunoglobulin (IgG, IgM, etc.). This technology is used to study the structure, function, and interaction with antigens of antibodies, and is the basis of immunology research. It is also used to prepare antibodies in diagnostic kits, such as enzyme-linked immunosorbent assay (ELISA), for disease detection and diagnosis, etc.

[0003] Among the many protein antibody purification methods, purification by specific binding of protein A or protein G to the Fc segment of the antibody is a common and effective method. The key consumable for this purification process is the chromatography column, also known as the purification column. Chromatography columns are mainly divided into pre-packed columns and empty columns. Although pre-packed columns are convenient to use, their internal packing and structure are fixed and cannot be adjusted or optimized according to experimental requirements. In contrast, empty columns, as general-purpose purification columns, have more extensive applicability and flexibility. When using an empty column for purification, the lower gasket, packing, and upper gasket need to be placed in the empty column in sequence before the purification can begin.

[0004] However, in actual operation, the placement of the lower gasket faces many challenges. To ensure purification effectiveness, the lower gasket must be tightly fitted against the inner bottom wall of the empty column, which is a critical step. However, the design of traditional empty columns is mostly elongated with a small diameter and a large depth, making it extremely inconvenient for operators to place the lower gasket. Tools such as tweezers are needed to repeatedly adjust the position inside the empty column to ensure the accuracy of the lower gasket. Not only is this operation tedious and time-consuming, but it also easily leads to displacement or damage of the lower gasket due to improper handling, which affects the stability of the entire purification process and the final purity of the antibody. SUMMARY

[0005] The utility model discloses a kind of general protein antibody purification columns to solve the problems existing in prior art, by the segmented design purification column, let operator can more conveniently place lower gasket into purification column.

[0006] To achieve the above object, the utility model adopts the following technical scheme: a general protein antibody purification column, comprising a hollow column, a bottom column is detachably arranged at one end of the hollow column, a discharge pipe is arranged at the end of the bottom column away from the hollow column, a funnel-shaped opening is formed in the inner bottom wall of the bottom column, and the opening is communicated with the discharge pipe.

[0007] By the above technical scheme, the traditional integrated purification column is designed into a segmented structure, that is, comprising a hollow column body and a bottom column, when placing the lower gasket, the bottom column can be first disassembled from the hollow column body, thereby reducing the length of the bottom column, so that the operator can more conveniently place the lower gasket into the bottom column, and after placing the lower gasket, the bottom column and the hollow column body can be quickly reconnected, thereby effectively solving the problem that in the traditional purification column, when placing the lower gasket, due to the small diameter and long depth of the hollow column, the step of placing the lower gasket is relatively troublesome, in addition, the bottom wall in the bottom column is provided with a funnel-shaped opening communicated with the discharge pipe, and the design of the funnel-shaped opening can enable the leaked substances to quickly pass through the discharge pipe and be discharged.

[0008] Optionally, the connection part of the hollow column body and the bottom column is provided with a mutually nested butt joint ring groove, and the butt joint ring groove is provided with a thread, so that the hollow column body and the bottom column are connected by screwing, which is simple in connection mode and can ensure the sealing effect.

[0009] Optionally, the outer periphery of the bottom column is provided with a plurality of damping protrusions, which facilitates the operator to unscrew or tighten the bottom column.

[0010] Optionally, the outer periphery of the end of the hollow column body away from the bottom column is provided with a limiting plate, the limiting plate is provided with an uncovering notch, and the end of the hollow column body away from the bottom column is provided with a cover in contact with the limiting plate.

[0011] The above technical scheme, by setting the limiting ring, the limiting ring can be used for limiting on the support without clamping structure, and by opening the uncovering notch on the limiting ring, the operator can conveniently uncover the cover.

[0012] Optionally, the outer surface of the hollow column body is symmetrically provided with two damping plates, one side of the damping plate away from the surface of the hollow column body is a plane, and the plane is provided with an anti-skid pattern.

[0013] In the above technical scheme, two damping plates are symmetrically arranged on the outer side of the hollow column body, which facilitates the fixation of the hollow column body on the support with a clamping member.

[0014] Optionally, it further comprises a lower gasket placed in the bottom column, the lower gasket is stacked with a filler having a height exceeding the bottom column, and an upper gasket is placed above the filler.

[0015] Optionally, the surface of the bottom column is sleeved with a sealing cap, and the inner wall of the sealing cap is provided with a sealing rubber ring. By arranging the sealing cap, it is avoided that sundries enter the inside of the purification column when the product is not used.

[0016] Optionally, the outer surface of the hollow column body is provided with a scale line, and the scale line can enable the operator to intuitively control the progress of the filler filling, thereby avoiding overfilling of the filler.

[0017] Compared with the prior art, the utility model discloses the beneficial effect is: 1, the traditional integrated purification column is designed into sectional structure, namely containing hollow cylinder and bottom column, when placing lower gasket, can first break apart bottom column and hollow cylinder, by this reduce the length of bottom column, make the operator more conveniently put lower gasket into bottom column, after placing lower gasket, can quickly reconnect bottom column and hollow cylinder, effectively solved the traditional purification column when placing lower gasket, because the diameter of empty column is small and the depth is long, lead to the step of placing lower gasket is more troublesome problem, 2, bottom column inner bottom wall is provided with funnel -shaped through -opening, the through -opening is connected with the drain pipe, can make the material that leaks down quickly pass through drain pipe and remove, 3, bottom column's outer periphery is equipped with a plurality of damping protruding, convenient for operator to unscrew or tighten bottom column, 4, through setting up uncovering gap on the limiting ring, convenient for operating personnel to uncover the seal cover. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 A general type protein antibody purification column overhead angle three-dimensional schematic view is provided for the utility model embodiment;

[0019] Figure 2 A general type protein antibody purification column cut surface three-dimensional schematic view is provided for the utility model embodiment;

[0020] Figure 3 A general type protein antibody purification column overhead angle three-dimensional schematic view is provided for the utility model embodiment;

[0021] Figure 4 A general type protein antibody purification column filler schematic view is provided for the utility model embodiment;

[0022] Figure 5 The hollow cylinder and bottom column separation state schematic view is provided for the utility model embodiment.

[0023] In the drawing: 1, hollow cylinder;11, first annular groove;2, bottom column;21, drain pipe;22, damping protruding;23, second annular groove;24, scale ruler line;3, seal cap;4, limiting plate;41, uncovering gap;5, seal cover;6, damping plate;7, lower gasket;8, filler;9, upper gasket. DETAILED DESCRIPTION

[0024] The technical scheme of the utility model will be described clearly and completely below in combination with the drawings in the utility model, and obviously, the described embodiment is only a part of the embodiment of the utility model, not all the embodiment. Based on the embodiment in the utility model, all other embodiments obtained by the ordinary skill in the art without making creative labor belong to the scope of protection of the utility model.

[0025] In the description of the utility model, it is necessary to explain, the terms "intermediate", "upper", "lower", "left", "right", "internal", "external" and the like indicate the orientation or position relationship based on the orientation or position relationship shown in the drawing, only for the convenience of describing the utility model and simplifying the description, and not indicating or implying that the indicated device or element must have a specific orientation, be constructed and operated in a specific orientation, therefore, it cannot be understood as a limitation on the utility model.

[0026] As Figure 1 The specific scheme of the embodiment is as follows: a general protein antibody purification column, comprising a hollow column body 1, a bottom column 2 detachably arranged at one end of the hollow column body 1, a discharge pipe 21 arranged at the end of the bottom column 2 away from the hollow column body 1, a funnel-shaped through hole arranged in the inner bottom wall of the bottom column 2, and the through hole being in communication with the discharge pipe 21.

[0027] In addition, the general protein antibody purification column further comprises a lower gasket 7 placed in the bottom column 2, a filler 8 with a height exceeding that of the bottom column 2 stacked on the lower gasket 7, and an upper gasket 9 placed above the filler 8.

[0028] In the embodiment, the lower gasket 7 is placed in the bottom column 2, and the main function thereof is to support the chromatography medium and prevent the medium from moving or collapsing during the flow process, so as to ensure the stability and separation effect of the chromatography column. The lower gasket 7 is made of stainless steel, has a long service life, is not easy to be damaged or deformed, and can be repeatedly used after being cleaned and disinfected, which is economical and practical. The stainless steel gasket has a net structure and can maintain stability under various buffer and elution conditions, and will not chemically react with the sample or medium, thereby ensuring the reliability and safety of the purification process.

[0029] The filler 8 is the core part of the purification column, and the performance thereof directly affects the purification effect. The filler 8 is mainly an affinity chromatography medium. In the technical scheme, the filler 8 in the embodiment is the protein A or protein G agarose medium on the market. The height of the filler 8 exceeds that of the bottom column 2, so as to ensure sufficient contact area and separation space during the purification process, and improve the efficiency and purity of the purification.

[0030] The upper gasket 9 is placed above the filler 8, and the main function thereof is to compress the filler 8, prevent the filler 8 from moving or deforming during the flow process, and ensure the uniformity and stability of the filler 8. The upper gasket 9 is made of similar materials and structures as the lower gasket 7, has good mechanical properties and chemical stability, can withstand a certain pressure and impact, and ensures the smooth progress of the purification process.

[0031] As Figure 2 , Figure 4 As shown in the drawings, a butt joint ring groove is arranged at the connection between the hollow column body 1 and the bottom column 2, the butt joint ring groove is nested with the butt joint ring groove, a thread is arranged on the butt joint ring groove, the hollow column body 1 and the bottom column 2 are connected by the thread, the connection mode is simple and can ensure the sealing effect.

[0032] like Figure 5 As shown, specifically, the docking groove includes a first annular groove 11 formed on the outer wall of the top of the bottom column 2 and a second annular groove 23 formed on the inner wall of the bottom of the hollow column 1. To ensure the sealing effect between the hollow column 1 and the bottom column 2, a sealing ring is also provided on the end face of the first annular groove 11. This sealing ring is made of medical-grade silicone, which not only ensures the sealing effect but also effectively resists the corrosion of various reagents.

[0033] In this embodiment, a plurality of damping protrusions 22 are provided on the outer periphery of the base column 2. Preferably, the damping protrusions 22 are hemispherical plastic protrusions with a frosted surface, which makes it convenient for the operator to unscrew or tighten the base column 2.

[0034] like Figure 3 As shown, a limiting plate 4 is provided on the outer periphery of the hollow column 1 at the end away from the bottom column 2. By setting a limiting ring, the limiting ring can be used to limit the position on the bracket without a clamping structure. A cover 5 is provided at the end of the hollow column 1 away from the bottom column 2, which contacts the limiting plate 4. The bottom of the cover 5 is provided with a plastic plug that can be embedded in the hollow column 1 to prevent debris from entering the hollow column 1. A cover opening notch 41 is provided on the limiting plate 4. The cover opening notch 41 is an arc-shaped notch with rounded chamfers at both ends, which makes it convenient for the operator to open the cover 5.

[0035] In this embodiment, two damping plates 6 are symmetrically arranged on the outer surface of the hollow column 1. The side of the damping plate 6 away from the surface of the hollow column 1 is flat and has anti-slip texture.

[0036] In the above technical solution, by symmetrically setting two damping plates 6 on the outside of the hollow column 1, it is convenient to fix the hollow column 1 on the bracket with clamping parts.

[0037] It should be noted that the upper end of the damping plate 6 is in contact with the limiting plate 4. In this way, after clamping, it can be further limited by the limiting rod to prevent the purification column from sliding down.

[0038] In this embodiment, a cap 3 is fitted onto the surface of the bottom column 2, and a sealing ring is provided on the inner wall of the cap 3. By setting the cap 3, impurities are prevented from entering the purification column when the product is not in use.

[0039] In this embodiment, a scale line 24 is provided on the outer surface of the hollow cylinder 1. The scale line 24 allows the operator to intuitively control the filling progress of the filler 8 and avoid overfilling. Furthermore, to make the scale line 24 more conspicuous and easier to read, the scale line 24 is made of a color that is significantly different from the color of the hollow cylinder 1 itself, such as black or red.

[0040] The working principle of the general protein antibody purification column is based on chromatography technology, and the separation and purification of the antibody are realized through the interaction between the filler 8 and the target antibody.

[0041] The specific operation process is as follows: first, the bottom column 2 of the purification column is separated from the hollow column body 1, the lower gasket 7 is placed in the bottom column 2, then the bottom column 2 is reconnected with the hollow column body 1, the sealing property of the connection is ensured, then the filler 8 is poured into the lower gasket 7 from the top port of the hollow column body 1, and the height of the filler 8 should exceed the bottom column 2. Then, the upper gasket 9 is placed above the filler 8, the filler 8 is compressed, and then the subsequent operation processes such as column balancing, sample loading, washing, elution and regeneration preservation can be started.

[0042] In the embodiment, the traditional integrated purification column is designed into a segmented structure, including the hollow column body 1 and the bottom column 2. This design makes it possible to first separate the bottom column 2 from the hollow column body 1 when placing the lower gasket 7, reduces the length of the bottom column 2, and makes it more convenient for the operator to place the lower gasket 7 in the bottom column 2. After placing the lower gasket 7, the bottom column 2 and the hollow column body 1 can be quickly reconnected, effectively solving the problem that the traditional purification column is difficult to place the lower gasket 7 due to the small diameter and long depth of the hollow column, improving the convenience and efficiency of the operation. In addition, the funnel-shaped opening is provided in the inner bottom wall of the bottom column 2 and communicates with the discharge pipe 21, so that the leaked substances can quickly pass through the discharge pipe 21 and be discharged. This design avoids the accumulation of waste liquid or impurities in the purification column, prevents blockage and pollution, and ensures the smooth progress of the purification process and the reliability of the purification result.

[0043] Although the embodiments of the utility model have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to these embodiments without departing from the principles and spirits of the utility model, and the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A general protein antibody purification column comprising a hollow cylinder, characterized in that: The hollow cylinder is detachably provided with a bottom column at one end, the bottom column is provided with a discharge pipe away from the one end of the hollow cylinder, a funnel-shaped through hole is formed in the inner bottom wall of the bottom column, and the through hole is communicated with the discharge pipe.

2. The general protein antibody purification column according to claim 1, characterized in that: The connecting part of the hollow cylinder and the bottom column is provided with a mutually nested butt joint ring groove, and the butt joint ring groove is provided with a screw thread.

3. The universal protein antibody purification column according to claim 1, characterized in that: The outer periphery of the bottom column is provided with a plurality of damping protrusions.

4. The universal protein antibody purification column according to claim 1, characterized in that: The outer periphery of the one end of the hollow cylinder away from the bottom column is provided with a limiting plate, a cover opening is formed in the limiting plate, and the one end of the hollow cylinder away from the bottom column is provided with a cover in contact with the limiting plate.

5. The general protein antibody purification column according to claim 1, characterized in that: The outer surface of the hollow cylinder is symmetrically provided with two damping plates, the side away from the surface of the hollow cylinder of the damping plate is a plane, and an anti-skid line is formed.

6. The general protein antibody purification column according to claim 1, characterized in that: A lower gasket is further placed in the bottom column, the lower gasket is stacked with a filler with a height exceeding that of the bottom column, and an upper gasket is placed above the filler.

7. The general protein antibody purification column according to claim 1, characterized in that: The surface of the bottom column is sleeved with a sealing cap, and the inner wall of the sealing cap is provided with a sealing rubber ring.

8. The general protein antibody purification column according to claim 1, characterized in that: The outer surface of the hollow cylinder is provided with a scale line.

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