Excitation light source assembly
By setting an adjustment unit and a uniform light unit in the excitation optical path, the problem of uneven brightness of the excitation beam spot is solved, the light utilization rate and the image uniformity of the imaging device are improved, and the accuracy and consistency of the signal value of the micro-reaction unit are ensured.
Patent Information
- Application Number
- CN202520299773.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-24
- Publication Date
- 2025-12-30
- Estimated Expiration
- 2035-02-24
AI Technical Summary
In existing technologies, the excitation light source components have a brightness difference between the edge field of view and the center field of view, resulting in uneven brightness of the light spot formed by the excitation beam on the chip, which affects the accuracy and consistency of the signal value of the micro-reaction unit.
By setting an adjustment unit and a homogenizing unit in the excitation optical path, the adjustment unit shrinks the divergence angle of the excitation beam, and the homogenizing unit performs homogenization processing to ensure that the area of the beam region is less than or equal to the opening area of the incident surface of the homogenizing unit, thus forming a light spot with uniform brightness and darkness.
This improves the light utilization rate of the excitation light source component, ensures the uniformity of brightness in the chip image acquired by the imaging device, and enhances the accuracy and consistency of the signal values of the micro-reaction unit.
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Figure CN223742876U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the field of optical technology, and particularly to an excitation light source assembly. BACKGROUND
[0002] The digital polymerase chain reaction (DPCR) technology needs to use an excitation light source assembly to irradiate a chip loaded with a large number of and independent micro-reaction units, and to obtain a fluorescence beam reflected by the micro-reaction units after absorbing the excitation light beam of the excitation light source assembly through an imaging device (such as a CCD camera) to determine the fluorescence signal value of the micro-reaction unit.
[0003] However, the excitation light source assembly in the related art has a bright-dark difference between the edge field of view and the center field of view, so that the light spot formed by the excitation light beam of the excitation light source assembly on the chip is uneven in brightness, resulting in that the chip image finally presented by the imaging device is uneven in brightness, and finally affecting the accuracy and consistency of determining the signal value of the micro-reaction unit. CONTENT OF THE UTILITY MODEL
[0004] The present application provides an excitation light source assembly, aiming at solving the problem of uneven brightness of the light spot formed by the excitation light beam in the related art.
[0005] To achieve the above-mentioned purpose:
[0006] The embodiment of the present application provides an excitation light source assembly, comprising:
[0007] An excitation light source, configured to generate an excitation light beam;
[0008] A light homogenizing unit, located on an optical path of the excitation light beam, configured to homogenize the excitation light beam;
[0009] An adjusting unit, located between the excitation light source and the light homogenizing unit, and located on the optical path, configured to contract the divergence angle of the excitation light beam, and make the area of the light beam region of the excitation light beam after passing through the adjusting unit and reaching the light homogenizing unit less than or equal to the opening area of the light entrance surface of the light homogenizing unit.
[0010] In one of the embodiments, the light homogenizing unit comprises a microarray lens, which is arranged on the side of the adjusting unit away from the excitation light source, and located on the optical path.
[0011] In one of the embodiments, the light homogenizing unit further comprises a focusing lens, which is arranged on the side of the micro-lens array away from the excitation light source and located on the light path, and the focusing lens is used to focus the excitation light beam emitted from the micro-lens array so as to form a target light spot on the focusing plane of the focusing lens.
[0012] In one of the embodiments, the excitation light source assembly further comprises a filter, which is located on the light path.
[0013] In one of the embodiments, the light entrance surface of the micro-lens array comprises a plurality of first micro-lenses, and the light exit surface of the micro-lens array comprises a plurality of second micro-lenses, each of the first micro-lenses corresponds to one of the second micro-lenses, the shape of the first micro-lenses is the same as that of the second micro-lenses, and the size of the first micro-lenses is the same as that of the second micro-lenses; wherein,
[0014] the size of the first micro-lenses in a preset direction satisfies a first preset relationship, and the first preset relationship is:
[0015] P lA ≥(D FT1 ×f lA1 ) / f Fl ;
[0016] P lA is the size of the first micro-lenses in a preset direction, D FT1 is a preset value corresponding to the preset direction, f lA1 is the focal length of the first micro-lenses, and f Fl is the focal length of the focusing lens.
[0017] In one of the embodiments, the adjusting unit comprises:
[0018] a converging lens group, which comprises at least two converging lenses, and is located on the light path, and is used to shrink the divergence angle of the excitation light beam incident to the converging lens group;
[0019] a collimating lens, which is located on the light path and on the side of the converging lens group away from the excitation light source, and is used to parallelize the excitation light beam incident to the collimating lens.
[0020] In one of the embodiments, the converging lens group comprises:
[0021] a first converging lens;
[0022] a second converging lens, which is located on a side of the first converging lens away from the excitation light source; wherein
[0023] a first divergence angle of the excitation light beam in the first converging lens is greater than a second divergence angle of the excitation light beam in the second converging lens.
[0024] In one of the embodiments, the converging lens group further comprises a third converging lens, which is located on a side of the second converging lens away from the excitation light source, and a third divergence angle of the excitation light beam in the third converging lens is less than the second divergence angle.
[0025] In one of the embodiments, a first difference between the first divergence angle and the second divergence angle is greater than or equal to a second difference between the second divergence angle and the third divergence angle.
[0026] In one of the embodiments, a light beam area of the excitation light beam in a first plane is located on an entrance surface of the third converging lens, wherein the first plane is a spatial plane in which the entrance surface of the third converging lens is located.
[0027] In one of the embodiments, a light beam area of the excitation light beam in a second plane is located on an entrance surface of the first converging lens, wherein the second plane is a spatial plane in which the entrance surface of the first converging lens is located.
[0028] a light beam area of the excitation light beam in a third plane is located on an entrance surface of the second converging lens, wherein the third plane is a spatial plane in which the entrance surface of the second converging lens is located.
[0029] In one of the embodiments, the excitation light source assembly further comprises a filter, which is located on the light path and between the converging lens group and the collimating lens.
[0030] In the excitation light source assembly provided in the present application, the adjusting unit and the light homogenizing unit are sequentially arranged in a direction away from the excitation light source and are located on a light path of the excitation light beam generated by the excitation light source. The adjusting unit shrinks the divergence angle of the excitation light beam emitted from the excitation light source, and when the excitation light beam passes through the adjusting unit and reaches the light homogenizing unit, the area of the light beam area of the excitation light beam is less than or equal to the opening area of the entrance surface of the light homogenizing unit. In this way, the adjusting unit only shrinks the divergence angle of the excitation light beam without converging the excitation light beam, and then the light homogenizing unit performs light homogenizing treatment on the excitation light beam, and finally the light spot formed by the excitation light beam after the light homogenizing treatment is bright and dark uniformly. BRIEF DESCRIPTION OF DRAWINGS
[0031] Figure 1 A structural schematic diagram of an excitation light source assembly provided for an embodiment of the present application is shown in FIG. 1.
[0032] Figure 2 A structural schematic diagram of an excitation light source assembly provided for another embodiment of the present application is shown in FIG. 2. Figure 1 A schematic diagram of the divergence angle of the excitation light source in FIG. 1 is shown in FIG. 3.
[0033] Figure 3 A structural schematic diagram of an excitation light source assembly provided for another embodiment of the present application is shown in FIG. 2.
[0034] Figure 4 A structural schematic diagram of an excitation light source assembly provided for another embodiment of the present application is shown in FIG. 2.
[0035] Figure 5 A structural schematic diagram of an excitation light source assembly provided for another embodiment of the present application is shown in FIG. 2. Figure 4 A structural schematic diagram of an excitation light source assembly provided for another embodiment of the present application is shown in FIG. 2. DETAILED DESCRIPTION
[0036] The utility model will be further described in detail below through specific embodiments in combination with the drawings. Similar elements in different embodiments adopt relevant similar element labels. In the following embodiments, many details are described in order to make the present application be better understood. However, those skilled in the art can easily recognize that part of the features can be omitted in different cases, or can be replaced by other elements, materials, methods. In some cases, some operations related to the present application are not shown or described in the specification in order to avoid the core part of the present application being overwhelmed by too much description, and for those skilled in the art, detailed description of the related operations is not necessary, and they can completely understand the related operations according to the description in the specification and general technical knowledge in the art.
[0037] In addition, the features, operations or characteristics described in the specification can be combined in any appropriate way to form various embodiments. At the same time, the steps or actions in the method description can also be sequentially changed or adjusted in a manner obvious to those skilled in the art. Therefore, the order in the specification and the drawings is only for clear description of an embodiment, and does not mean the necessary order, unless otherwise stated that a certain order must be followed.
[0038] The serial numbers of components in this paper, such as "first", "second", etc., are only used to distinguish the described objects, and have no order or technical meaning. Unless otherwise specified, the "connection" in the present application includes direct and indirect connection.
[0039] In an embodiment provided by the present application, as shown in FIG. 1, the excitation light source assembly 100 includes an excitation light source 10, an adjusting unit 20 and a light uniformizing unit 30. Figure 1
[0040] The excitation light source 10 is used to generate an excitation beam. The excitation light source 10 may include one or more LED beads, and this embodiment does not limit the number of LED beads included in the excitation light source 10.
[0041] In this embodiment, the excitation beam is a collection of multiple excitation beams emitted from the excitation light source 10.
[0042] The homogenizing unit 30 is located on the optical path of the excitation beam, that is, the homogenizing unit 30 is located on the light-emitting surface of the excitation source 10, and the excitation beam of the excitation source 10 can be incident on the homogenizing unit 30.
[0043] The homogenizing unit 30 is used to homogenize the excitation beam, that is, the light spot formed by the excitation beam emitted from the homogenizing unit 30 is uniform in brightness and darkness, which facilitates subsequent imaging processing using the homogenized light spot and ensures that the final image obtained by the imaging processing is uniform in brightness and darkness.
[0044] The adjustment unit 20 is located between the excitation light source 10 and the homogenizing unit 30. The adjustment unit 20 is located on the optical path of the excitation beam, that is, the adjustment unit 20 is located on the light-emitting surface of the excitation light source 10, and the excitation beam of the adjustment unit 20 can be incident on the adjustment unit 20.
[0045] The adjustment unit 20 is used to reduce the divergence angle of the excitation beam, and to ensure that when the excitation beam reaches the homogenizing unit 30 after passing through the adjustment unit 20, the area of the beam region of the excitation beam is less than or equal to the opening area of the incident surface of the homogenizing unit 30. Thus, in this embodiment, the adjustment unit 20 only reduces the divergence angle of the excitation beam without causing light convergence, thereby maximizing the collection of excitation light emitted from the excitation source 10.
[0046] like Figure 2 As shown, taking the excitation light source 10 as an example, in this embodiment, the divergence angle refers to the angle between the excitation rays at the edge of the excitation beam during its propagation. Figure 2 L11 in the middle) and the excitation ray at the center ( Figure 2 The angle a0 between L1 and L2 in the equation. The divergence angle is also known as the half-divergence angle.
[0047] The size of the divergence angle reflects the degree of divergence of the excitation beam. The larger the divergence angle, the more widely the excitation beam spreads during propagation, and the worse the light intensity and directionality of the excitation beam.
[0048] In the excitation light source assembly 100 provided in the embodiment, if the adjustment unit 20 is not arranged to contract the divergence angle of the excitation light beams directly emitted from the excitation light source 10, the number of excitation light rays incident to the light homogenizing unit 30 will be greatly reduced, thereby causing the light utilization rate of the excitation light source assembly 100 to be low, and further causing the brightness of the light spot formed by the excitation light beams emitted from the excitation light source assembly 100 to be insufficient. In this way, the number of LED lamp beads included in the excitation light source 10 needs to be increased.
[0049] On the contrary, the embodiment is based on the arrangement of the adjustment unit 20, so that a large number of excitation light rays directly emitted from the excitation light source 10 can be incident to the light homogenizing unit 30 for processing. In this way, the light utilization rate of the excitation light source assembly 100 is improved, and the brightness of the light spot formed by the excitation light beams emitted from the excitation light source assembly 100 is ensured to be sufficient, without the need to additionally increase the LED lamp beads.
[0050] Further, in an embodiment, as shown in Figure 3 the excitation light source assembly 100 further includes a filter 40, which is located on the light path formed by the excitation light beams. That is, in a specific embodiment, the filter 40 can be arranged between the excitation light source 10 and the adjustment unit 20. In another specific embodiment, the filter 40 can be arranged between the adjustment unit 20 and the light homogenizing unit 30. In yet another specific embodiment, the filter 40 can be arranged on the side of the light homogenizing unit 30 away from the excitation light source 10. In yet another specific embodiment, the filter 40 can be arranged between the micro-lens array 31 and the focusing lens 32.
[0051] The filter 40 is used to allow excitation light rays within a specific wavelength range to pass through, and to reflect or absorb excitation light rays outside the specific wavelength range. Based on the arrangement of the filter 40, the wavelength of the excitation light beams emitted from the excitation light source assembly 100 is selectively controlled.
[0052] Specifically, in an embodiment, as shown in Figure 4 the light homogenizing unit 30 includes a micro-lens array 31. The micro-lens array 31 is arranged on the side of the adjustment unit 20 away from the excitation light source 10, and the micro-lens array 31 is located on the light path formed by the excitation light beams.
[0053] The micro-lens array 31 is an array composed of a plurality of micron-level lenses arranged in a regular manner, and has a highly integrated feature. When the excitation light beams pass through the micro-lens array 31, the excitation light beams are split multiple times by the micro-lens array 31, thereby improving the light homogenizing effect of the excitation light beams emitted from the micro-lens array 31.
[0054] Exemplarily, the micro-lens array 31 can be a compound eye lens. Each micro-lens unit of the compound eye lens has independent focal length and imaging capability. Since the arrangement of the compound eye lens imitates the structure of the compound eye of insects, the compound eye lens has high spatial resolution and large field of view, so that the excitation light source assembly 100 provided by the embodiment can be adapted to high-resolution microscopic uniform imaging.
[0055] Further, in an embodiment, please continue to refer to Figure 4 The homogenization unit 30 further comprises a focusing lens 32. The focusing lens 32 is arranged on the side of the micro-lens array 31 away from the excitation light source 10, and the focusing lens 32 is located on the light path of the excitation light beam. The focusing lens 32 is used to focus the excitation light beam emitted from the micro-lens array 31, so that the excitation light beam emitted from the micro-lens array 31 forms a target light spot on the focusing surface of the focusing lens 32.
[0056] In this way, the target light spot is clear and the bright and dark are uniform. If the chip is placed on the focusing surface of the focusing lens, the subsequent imaging device can obtain a clear and uniformly bright chip image.
[0057] Further, in an embodiment, please continue to refer to Figure 4 The light-in surface of the micro-lens array 31 comprises a plurality of first micro-lenses 311, and the light-out surface of the micro-lens array 31 comprises a plurality of second micro-lenses 313. Each first micro-lens 311 corresponds to a second micro-lens 313, and the shape and size of the first micro-lens 311 are the same as those of the second micro-lens 313. In this way, when the laser beam irradiates the first micro-lens 311 on the compound eye lens 410, the more the number of the first micro-lenses 311 irradiated, the more times the laser beam is homogenized.
[0058] In the first preset relationship (1), P lA is the size of the first micro-lens 311 in the preset direction, D FT1 is a preset value corresponding to the preset direction, f lA1 is the focal length of the first micro-lens 311, and f Fl is the focal length of the focusing lens 32.
[0059] P lA ≥(D FT1 ×f lA1) / f Fl (1);
[0060] In the first preset relationship (1), P lA is the size of the first micro-lens 311 in the preset direction, D FT1 is a preset value corresponding to the preset direction, f lA1 is the focal length of the first micro-lens 311, and f Fl is the focal length of the focusing lens 32.
[0061] That is, in the present embodiment, if the focal length of the first microlens 311, the focal length of the focusing lens 32, and the size of the target spot in the preset direction (i.e., D FT1 ) are determined, the size of each first microlens 311 in the preset direction in the microlens array 31 can be selected by the above-mentioned first preset relationship formula (1) to make the area of the target spot formed by the excitation light beam passing through the microlens array 31 and the focusing lens 32 not less than the actual area of the chip, ensuring that any region of the chip can be irradiated by the excitation light beam forming the target spot.
[0062] It is worth mentioning that the shape of the target spot can be adjusted according to the shape of the first microlens 311, for example, when the cross section of the first microlens 311 is rectangular along the direction perpendicular to the optical axis of the first microlens 311, the shape of the target spot is rectangular. Similarly, when the cross section of the first microlens 311 is circular along the direction perpendicular to the optical axis of the first microlens 311, the shape of the target spot is circular.
[0063] Specifically, in an embodiment, as shown in Figure 3 and Figure 4 , the adjusting unit 20 includes a converging lens group 21 and a collimating lens 22.
[0064] The converging lens group 21 includes at least two converging lenses, and the converging lens group 21 is located on the optical path of the excitation light beam, for shrinking the divergence angle of the excitation light beam incident to the converging lens group 21.
[0065] It is difficult for a single converging lens to both collect all excitation light rays emitted from the excitation light source 10 and control the divergence angle of all excitation light rays to be zero. In the present embodiment, the converging lens group 21 is arranged to collect as many excitation light rays emitted from the excitation light source 10 as possible and gradually shrink the divergence angle of the excitation light beam.
[0066] The collimating lens 22 is located on the optical path of the excitation light beam, and the collimating lens 22 is located on the side of the converging lens group 21 away from the excitation light source 10, for parallelizing the excitation light beam incident to the collimating lens 22.
[0067] In the present embodiment, the collimating lens 22 focuses and adjusts the excitation light beam, so that the excitation light beam with a non-zero divergence angle is converted into a parallelized excitation light beam after passing through the collimating lens 22, i.e., into an excitation light beam with a divergence angle as small as possible or even tending to zero. The parallelized excitation light beam can still maintain a high light intensity and directionality at a relatively long distance.
[0068] Further, as shown in Figure 4As shown, the excitation light source assembly 100 further comprises a filter 40. The filter 40 is located on the light path of the excitation light beam, and the filter 40 is between the converging lens group 21 and the collimating lens 22.
[0069] In the embodiment, by arranging the filter 40 between the converging lens group 21 and the collimating lens 22, a filter 40 with a small light entrance area can be selected, the manufacturing cost of the filter 40 is saved, and the arrangement structure of each unit (the adjusting unit 20, the light homogenizing unit 30) of the excitation light source assembly 100 is more compact, and the excitation light source assembly 100 is miniaturized.
[0070] In a specific embodiment, as shown in Figure 4 The converging lens group 21 comprises a first converging lens 211 and a second converging lens 213, and the second converging lens 213 is located on the side of the first converging lens 211 away from the excitation light source 10.
[0071] As shown in Figure 4 and Figure 5 The first divergence angle a1 of the excitation light beam in the first converging lens 211 is greater than the second divergence angle a2 of the excitation light beam in the second converging lens 213.
[0072] The specific value of the first divergence angle a1 is related to the focal length of the first converging lens 211 and the distance between the first converging lens 211 and the excitation light source 10, and the specific value of the second divergence angle a2 is related to the focal length of the second converging lens 213 and the distance between the second converging lens 213 and the first converging lens 211. The focal length of the first converging lens 211, the focal length of the second converging lens 213, the distance between the first converging lens 211 and the excitation light source 10, and the distance between the second converging lens 213 and the first converging lens 211 are adjusted to ensure that the excitation light beam emitted from the first converging lens 211 is incident to the second converging lens 213 as much as possible, and to control the edge excitation light rays emitted from the converging lens group 21 to be more and more inclined to be parallel to the middle excitation light rays.
[0073] Further, please continue to refer to Figure 4 The light beam region of the excitation light beam on the second plane S2 is located on the light entrance surface of the first converging lens 211, and the second plane S2 is a spatial plane on which the light entrance surface of the first converging lens 211 is located. In this way, it is ensured that the excitation light beam emitted from the excitation light source 10 is incident to the first converging lens 211, and the loss of excitation light rays existing in the excitation light beam passing through the first converging lens 211 is reduced or even avoided.
[0074] Furthermore, the light beam region of the excitation light beam on the third plane S3 is located on the light entrance surface of the second converging lens 213, and the third plane S3 is a spatial plane on which the light entrance surface of the second converging lens 213 is located.
[0075] In yet another embodiment, as shown in Figure 4 and Figure 5 , the converging lens group 21 further comprises a third converging lens 215. The third converging lens 215 is located on the side of the second converging lens 213 away from the excitation light source 10, and the third divergence angle a3 of the excitation light beam in the third converging lens 215 is smaller than the second divergence angle a2. In this way, it is ensured that the excitation light beam emitted from the first converging lens 211 is incident to the second converging lens 213, reducing or even avoiding the loss of excitation light rays passing through the second converging lens 213.
[0076] The specific value of the third divergence angle a3 is related to the focal length of the third converging lens 215 and the distance between the third converging lens 215 and the second converging lens 213. The focal length of the third converging lens 215 and the distance between the third converging lens 215 and the second converging lens 213 are adjusted accordingly to ensure that the excitation light beam emitted from the second converging lens 213 is incident to the third converging lens 215 as much as possible, further controlling the edge excitation light rays emitted from the converging lens group 21 to be more and more parallel to the middle excitation light rays.
[0077] In this embodiment, the converging lens group 21 composed of the first converging lens 211, the second converging lens 213 and the third converging lens 215 is easier to adjust the optical path formed by the excitation light beam, so as to maximize the collection of excitation light rays emitted from the excitation light source 10, while also being able to control the spot area formed by the excitation light beam passing through the first converging lens 211, the spot area formed by the excitation light beam passing through the second converging lens 213 and the spot area formed by the excitation light beam passing through the third converging lens 215, respectively.
[0078] Further, please continue to refer to Figure 4 and Figure 5 , the first difference between the first divergence angle a1 and the second divergence angle a2 is greater than or equal to the second difference between the second divergence angle a2 and the third divergence angle a3.
[0079] In this embodiment, when the excitation light beam passes through the converging lens group 21, the divergence angle of the excitation light beam gradually shrinks, and the degree of divergence angle shrinkage gradually flattens, which facilitates controlling the area of the light beam region formed by the excitation light beam incident to the homogenizing unit 30 to be larger than the area of the light beam region formed by the excitation light beam incident to the adjusting unit 20.
[0080] In a specific embodiment, the light beam region of the excitation light beam on the first plane S1 is located on the light entrance surface of the third converging lens 215, and the first plane S1 is a spatial plane on which the light entrance surface of the third converging lens 215 is located. In this way, it is ensured that the excitation light beam emitted from the second converging lens 213 is incident to the third converging lens 215, reducing or even avoiding the loss of excitation light rays of the excitation light beam passing through the third converging lens 215.
[0081] The technical features of the above-described embodiments can be combined in any manner. For the sake of brevity, not all possible combinations of the technical features in the above-described embodiments are described, but it should be understood that any combination of the technical features is within the scope of the present disclosure as long as the combination does not result in contradictions.
[0082] The above merely describes specific embodiments of the present application, but the protection scope of the present application is not limited thereto. Any person skilled in the art can easily think of changes or replacements within the technical scope disclosed by the present application, and all such changes or replacements should be encompassed within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.
Claims
1. An excitation light source assembly, characterized by, The excitation light source is used to generate an excitation light beam. The uniform light unit is located on the light path of the excitation light beam, and is used to uniformly light the excitation light beam. The adjustment unit is located between the excitation light source and the uniform light unit, and is located on the light path. The adjustment unit is used to shrink the divergence angle of the excitation light beam, and make the area of the light beam region of the excitation light beam after passing through the adjustment unit and reaching the uniform light unit smaller than or equal to the opening area of the light entrance surface of the uniform light unit.
2. The excitation light source assembly of claim 1, wherein, The uniform light unit includes a micro-lens array, which is arranged on the side of the adjustment unit away from the excitation light source and located on the light path.
3. The excitation light source assembly of claim 2, wherein, The uniform light unit also includes a focusing lens, which is arranged on the side of the micro-lens array away from the excitation light source and located on the light path.
4. The excitation light source assembly according to any one of claims 1 to 3, characterized in that, The focusing lens is used to focus the excitation light beam emitted from the micro-lens array, so that the excitation light beam forms a target light spot on the focusing surface of the focusing lens.
5. The excitation light source assembly of claim 3, wherein, The excitation light source assembly also includes a filter located on the light path. The light entrance surface of the micro-lens array includes a plurality of first micro-lenses, and the light exit surface of the micro-lens array includes a plurality of second micro-lenses. P lA ≥(D FT1 ×f lA1 ) / f Fl ; P lA D is a size of the first microlens in a preset direction, FT1 f is a preset value corresponding to the preset direction, lA1 f is a focal length of the first microlens, Fl f is a focal length of the focusing lens.
6. The excitation light source assembly of claim 1, wherein, Each first micro-lens corresponds to a second micro-lens. The shape and size of the first micro-lens are the same as those of the second micro-lens. The size of the first micro-lens in a predetermined direction satisfies a first predetermined relationship, which is:
7. The excitation light source assembly of claim 6, wherein, The adjustment unit includes: A converging lens group including at least two converging lenses, which is located on the light path and used to shrink the divergence angle of the excitation light beam incident on the converging lens group. A collimating lens located on the light path and on the side of the converging lens group away from the excitation light source, which is used to parallelize the excitation light beam incident on the collimating lens. The converging lens group includes:
8. The excitation light source assembly of claim 7, wherein, A first converging lens; 9. The excitation light source assembly of claim 8, wherein, A second converging lens located on the side of the first converging lens away from the excitation light source; wherein 10. The excitation light source assembly of claim 8, wherein, The first divergence angle of the excitation light beam in the first converging lens is greater than the second divergence angle of the excitation light beam in the second converging lens. The converging lens group also includes a third converging lens located on the side of the second converging lens away from the excitation light source. The third divergence angle of the excitation light beam in the third converging lens is smaller than the second divergence angle. The first difference between the first divergence angle and the second divergence angle is greater than or equal to the second difference between the second divergence angle and the third divergence angle. The light beam region of the excitation light beam in a first plane is located on the light entrance surface of the third converging lens, wherein the first plane is a spatial plane in which the light entrance surface of the third converging lens is located.
11. The excitation light source assembly according to claim 7 or 8, wherein, a light beam region of the excitation light beam in a second plane is located on the entrance surface of the first converging lens, wherein the second plane is a spatial plane in which the entrance surface of the first converging lens is located; a light beam region of the excitation light beam in a third plane is located on the entrance surface of the second converging lens, wherein the third plane is a spatial plane in which the entrance surface of the second converging lens is located.
12. The excitation light source assembly of claim 6, wherein, The excitation light source assembly further comprises a filter, the filter is located on the optical path, and the filter is between the converging lens group and the collimating lens.