Branchiostoma breeder conservation and fry collection device

Abstract

The utility model provides a breed conservation and fry collection device for branchiostoma belcheri, and the device comprises a breeding mechanism, sea sand is laid at the bottom of the breeding mechanism, and the breeding mechanism is filled with seawater; a water outlet is formed in the bottom of the breeding mechanism, and a sealing mechanism is installed on the water outlet and used for sealing the water outlet; the water inlet mechanism is arranged in the culture mechanism, the water inlet mechanism is provided with a water inlet, and the water inlet is arranged below the water surface of seawater in the culture mechanism; the water outlet mechanism is arranged in the breeding mechanism, and a water outlet is formed in the upper portion of the water outlet mechanism; the first filter screen is mounted at the bottom of the breeding mechanism, and the first filter screen is arranged on the outer side of the water outlet.

Description

TECHNICAL FIELD

[0001] The present disclosure belongs to the technical field of broodfish, and relates to a broodfish breeding and fry collecting device. BACKGROUND

[0002] Broodfish is a chordate animal of the genus Branchiostoma in the family Branchiostomidae in the order Branchiostomatiformes. Broodfish is of great significance in academics because they are a transitional type from invertebrates to vertebrates, providing a valuable model for studying the origin and evolution of vertebrates. Due to overfishing and habitat destruction, the number of broodfish has once decreased, and currently, broodfish is listed as a national second-class protected animal in China, and corresponding natural protection zones are established to protect the living environment of broodfish.

[0003] Research and development of the broodfish breeding and fry collecting device can provide an effective way for the reproduction and protection technology of broodfish biological population, and have important significance. SUMMARY

[0004] The present disclosure provides a broodfish breeding and fry collecting device, which can effectively solve the above problems.

[0005] The present disclosure is implemented in this way:

[0006] The present disclosure provides a broodfish breeding and fry collecting device, which comprises:

[0007] A breeding mechanism, a bottom of the breeding mechanism is paved with sea sand, and the breeding mechanism is internally provided with seawater;

[0008] A drainage port is arranged at the bottom of the breeding mechanism, and a sealing mechanism is installed on the drainage port to seal the drainage port;

[0009] A water inlet mechanism is arranged inside the breeding mechanism, and the water inlet mechanism is provided with a water inlet port arranged below the water surface of the seawater in the breeding mechanism;

[0010] A water outlet mechanism is arranged inside the breeding mechanism, and the upper portion of the water outlet mechanism is provided with a water outlet port;

[0011] A first filter screen is installed at the bottom of the breeding mechanism, and the first filter screen is arranged outside the drainage port.

[0012] The present disclosure has the following beneficial effects:

[0013] The present disclosure provides a device for preserving and collecting amphioxus, which can preserve wild parents and simulate the natural growth environment of amphioxus. The device can realize the circulation and replacement of seawater containing algae in the breeding mechanism through the water inlet mechanism and the water outlet mechanism, and provide continuous food for amphioxus, thereby avoiding the high metabolic load caused by excessive food intake through timed feeding.

[0014] Further, the circulation and replacement of seawater containing algae, and the regular drainage through the drainage structure can simulate the self-purification of the marine environment, keep the seawater and sand in the breeding mechanism clean, and avoid affecting the growth and survival of amphioxus. BRIEF DESCRIPTION OF DRAWINGS

[0015] In order to more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings needed in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present disclosure, and therefore should not be considered as limiting the scope. For those skilled in the art, other related drawings can also be obtained without creative labor.

[0016] Figure 1 FIG. 1 is a first structural schematic diagram of the device for preserving and collecting amphioxus provided by the embodiments of the present disclosure.

[0017] Figure 2 FIG. 2 is a second structural schematic diagram of the device for preserving and collecting amphioxus provided by the embodiments of the present disclosure.

[0018] Figure 3 FIG. 3 is a structural schematic diagram of the storage mechanism of the device provided by the embodiments of the present disclosure.

[0019] Figure 4 FIG. 4 is a schematic diagram of the working of the collection mechanism, the water pumping mechanism and the aeration mechanism of the device provided by the embodiments of the present disclosure. DETAILED DESCRIPTION

[0020] In order to make the purposes, technical solutions and advantages of the embodiments of the present disclosure clearer, the technical solutions in the embodiments of the present disclosure will be described clearly and completely below with reference to the drawings in the embodiments of the present disclosure. Obviously, the described embodiments are only part of the embodiments of the present disclosure, rather than all the embodiments. Based on the embodiments in the present disclosure, all other embodiments obtained by a person of ordinary skill in the art without creative work fall within the protection scope of the present disclosure. Therefore, the following detailed description of the embodiments of the present disclosure provided in the drawings is not intended to limit the scope of the claimed present disclosure, but only represents selected embodiments of the present disclosure. Based on the embodiments in the present disclosure, all other embodiments obtained by a person of ordinary skill in the art without creative work fall within the protection scope of the present disclosure.

[0021] Embodiment 1

[0022] As shown in Figure 1 and 2 The present disclosure provides a device for breeding and collecting amphioxus, which comprises:

[0023] A breeding mechanism 1, the bottom of the breeding mechanism 1 is paved with sea sand 2, and the breeding mechanism 1 is filled with seawater 3.

[0024] In some embodiments, the breeding mechanism 1 is a PVC plastic bucket with a diameter of 1 m and a height of 60 cm. 5000-10000 amphioxus are put into the breeding mechanism 1.

[0025] The bottom of the breeding mechanism 1 is paved with sea sand 2 with a height of 5-10 cm, which is used to meet the habit of amphioxus burrowing in sand. The height of the sea sand 2 can bury the body of amphioxus.

[0026] The sea sand 2 is taken from the sea area where amphioxus lives.

[0027] The particle size of the sea sand 2 is 0.5-1 mm. The sea sand 2 is soaked in fresh water for 2 days and then washed with fresh seawater for 2 times.

[0028] The breeding mechanism 1 is filled with seawater 3. The water level of the seawater 3 is 50 cm.

[0029] The breeding mechanism 1 is placed in a breeding room to ensure the temperature of the seawater 3 in the breeding mechanism 1, which is 26-32℃.

[0030] The breeding room is provided with natural light with a natural light intensity cycle.

[0031] In some embodiments, the breeding mechanism 1 is provided with a support or a supporting leg to facilitate the arrangement of a drainage pipeline.

[0032] The drain pipeline is used for transmitting seawater out of the breeding mechanism 1 to a treatment facility.

[0033] The amphioxus living bodies are collected from natural sea areas as the parent population, and are bred in the device.

[0034] The collected wild amphioxus has different sizes, i.e., the development degrees of the parent individuals are different. The collected parent individuals are bred in the device, so that the individuals with lower gonad maturity development can reach the state of gonad fullness in the breeding process, and the breeding of different size individuals is realized.

[0035] The seawater 3 in the breeding mechanism 1 is seawater containing algae which is taken in by the water inlet mechanism 5.

[0036] The seawater containing algae is natural seawater containing algae. The use of natural seawater can better simulate the natural growth environment of amphioxus.

[0037] The seawater containing algae is obtained by filtering seawater in a seawater pond. The seawater containing algae is taken from the seawater pond.

[0038] The seawater pond is an open water system which is a pool dug in a reclamation beach, for example, an existing seawater pond shrimp pond can be used. The seawater in the seawater pond is filtered by a 300-mesh screen gauze to remove large particle substances such as sea sand and parasites, and then can be used.

[0039] The seawater in the seawater pond is obtained by pumping seawater meeting the breeding standards in a natural environment into the pond, and contains natural algae. The algae in the seawater containing algae can grow naturally.

[0040] In some embodiments, as shown in Figure 3 The device further comprises a water storage mechanism 8 which is in communication with the water inlet mechanism 5.

[0041] Specifically, the water storage mechanism 8 is a water tower. The volume of the water tower is 3-5 tons.

[0042] In some embodiments, the device is arranged in a breeding room.

[0043] That is, the water storage mechanism 8 and the breeding mechanism 1 are both placed in the breeding room.

[0044] In some embodiments, as shown in Figure 3 The water storage mechanism 8 is in communication with the seawater pond.

[0045] The seawater is first taken into the water storage mechanism 8 from the seawater pond, and is placed at a constant temperature before being used for water inlet of the water inlet mechanism 5.

[0046] As shown in Figure 1 and 2 The device comprises:

[0047] The water inlet mechanism 5 is arranged inside the breeding mechanism 1, and is provided with a water inlet 51 arranged below the water surface of the seawater 3 in the breeding mechanism 1.

[0048] In some embodiments, as shown in Figure 1 and 2 The water inlet mechanism is arranged along the circumference of the breeding mechanism 1, and the direction of the water inlet 51 is the same as the direction of the circumference of the breeding mechanism 1.

[0049] The seawater containing algae is taken into the breeding mechanism 1 through the water inlet mechanism 5.

[0050] The flow rate of the entering seawater can be observed to flow in the breeding mechanism 1.

[0051] In some embodiments, the water inlet mechanism 5 is a bent pipe structure.

[0052] The water inlet mechanism 5 is a PVC bent pipe with a diameter of 5 cm, and four are uniformly arranged along the circumference of the breeding mechanism 1. The bending direction is the same as the direction of the circumference, so that the entering seawater forms a circulating flow in the seawater in the breeding mechanism, and the seawater in the breeding mechanism is uniformly mixed.

[0053] In some embodiments, the bending angle of the water inlet bent pipe is 45°. The position of the water inlet is 20 cm below the water surface in the breeding mechanism 1.

[0054] The number of water inlet mechanisms, the bending angle of the water inlet bent pipe, the position of the water inlet, and the flow rate of the water inlet are related to the water height, cross section and volume in the breeding mechanism.

[0055] By entering seawater in the breeding mechanism, the flow of seawater is formed, which can simulate the tidal fluctuation in the natural marine environment.

[0056] Moreover, by continuously replacing the seawater in the breeding mechanism through water inlet and outlet, a circulation is formed, which can increase the dissolved oxygen in the water and improve the water quality.

[0057] The entering seawater is placed to a constant room temperature in the breeding chamber, and then enters the breeding mechanism 1.

[0058] In some embodiments, the device, the breeding chamber and the seawater pond provided by the embodiment of the present application constitute an ambystoma mexicanum breeding and harvesting system.

[0059] That is, the system comprises the device, the breeding chamber and the seawater pond.

[0060] As shown in Figure 1 and 2 The device comprises:

[0061] The water outlet mechanism 6 is arranged inside the breeding mechanism 1, and the upper part of the water outlet mechanism 6 is provided with a water outlet 61.

[0062] In some embodiments, the water outlet mechanism 6 is a straight pipe structure, which is installed at the bottom of the breeding mechanism 1 and connected to the drainage pipeline.

[0063] The water outlet mechanism 6 is specifically a PVC straight pipe with a diameter of 5 cm. A water outlet 61 is arranged at the corresponding position of the water surface for drainage. Water is drained at the same time as water is introduced, so as to maintain the water level in the breeding mechanism 1.

[0064] In other embodiments, the water outlet mechanism 6 can also be provided with multiple water outlets.

[0065] The feeding mode of circulating algal-containing seawater by introducing and draining water in the breeding mechanism 1 is a strategy for "rough feeding" of the brine shrimp.

[0066] The algal-containing seawater can continuously provide food for the brine shrimp, so that the brine shrimp can feed at any time, which is closer to the growth in the natural environment, rather than periodic nutritional impact, thereby improving the adaptation of the brine shrimp to the breeding environment and improving the survival rate of the brine shrimp.

[0067] The continuous feeding mode of the algal-containing seawater can also reduce the feeding amount of each feeding of the timed feeding, reduce the impact on the water quality caused by the timed feeding, such as excessive turbidity of the water body, so that the brine shrimp cannot adapt to the change of the breeding environment and cannot mate, and even die.

[0068] Correspondingly, the device is used for breeding the brine shrimp, and algal nutrient solution needs to be periodically fed every day to provide sufficient nutrients for the brine shrimp, which realizes "fine feeding" compared with "rough feeding" of the algal-containing seawater, so as to meet the growth and reproduction needs of the brine shrimp.

[0069] As shown in Figure 1 and 2 The bottom of the breeding mechanism 1 is provided with a drainage port 4. The drainage port is provided with a sealing mechanism 41, specifically a plug, for sealing the drainage port 4.

[0070] The diameter of the drainage port 4 is slightly larger than that of the PVC straight pipe for water outlet, which is 6 cm.

[0071] In some embodiments, the water outlet mechanism 6 is installed on the sealing mechanism 41.

[0072] The PVC straight pipe for water outlet can be directly installed on the plug and connected to the bottom of the plug, so that the plug can be pulled up by the PVC straight pipe to open the drainage port 4.

[0073] Further, the first filter screen 7 is installed on the bottom of the breeding mechanism 1. The diameter of the first filter screen 7 is slightly larger than the diameter of the drain 4, and is 7 cm.

[0074] The height of the first filter screen 7 needs to be 1-2 cm higher than the height of the sea sand 2, so as to isolate the sea sand 2 from being drained out of the drain 4. The side of the first filter screen 7 is provided with mesh holes, so that the seawater 3 in the sea sand 2 can pass through.

[0075] In some embodiments, the top of the first filter screen 7 is an open structure to provide space for the plug to be pulled out.

[0076] The purpose of the drainage is to simulate the rise and fall of the tide in the habitat of the amphioxus. By draining, the sea sand 2 is exposed to the water surface, and then the incoming seawater will again submerge the sea sand 2.

[0077] By draining, the seawater 3 in the breeding mechanism 1 can be completely replaced, thereby providing a better growth environment for the amphioxus.

[0078] Therefore, by using the mode of water circulation and periodic drainage, the amount of algal nutrient solution fed each time can be set at an upper limit without being controlled too accurately. Even if the amount of each feeding is slightly higher than the amount that can be consumed by the amphioxus, and there is a residue in the seawater, there is no need to worry about the cumulative effect of the concentration of nutrients such as algae in the seawater on the water quality after multiple overfeeding.

[0079] In some embodiments, as shown in FIG. 1, the device further comprises an aeration mechanism 9. Figure 4

[0080] The aeration mechanism 9 is arranged inside the breeding mechanism 1.

[0081] The aeration mechanism can be uniformly arranged along the circumference of the breeding mechanism 1, and 4 aeration mechanisms can be arranged to be able to observe the flow of seawater in the breeding mechanism 1.

[0082] The amount of aeration, the number of aeration mechanisms, and the aeration position are also related to the water height, cross section, and volume in the breeding mechanism 1.

[0083] After each feeding of the algal nutrient solution, the water inlet structure 5 needs to be stopped, specifically for 2 hours.

[0084] The purpose of stopping the water is to avoid the loss of the fed algal nutrient solution with the water outlet, so that the amphioxus can fully feed during this period and obtain the required nutrients for breeding.

[0085] The aeration mechanism 9 is used during the stopping of the water inlet mechanism 5 to make the seawater 3 in the breeding mechanism 1 uniform and improve the dissolved oxygen therein.

[0086] ​The aeration mechanism 9 is also used to aerate the seawater 3 when collecting the fertilized eggs, and the amount of aeration is determined according to the fact that no air wave is generated, so as to avoid disturbing the mating behavior of the amphioxus and causing damage to the fertilized eggs.

[0087] The aeration mechanism 9 is also used to aerate the seawater 3 when collecting the fertilized eggs, and the amount of aeration is determined according to the fact that no air wave is generated, so as to avoid disturbing the mating behavior of the amphioxus and causing damage to the fertilized eggs.

[0088] In some embodiments, as shown in Figure 4 The device further comprises:

[0089] The collecting mechanism 10 is arranged in the breeding mechanism 1, the top of the collecting mechanism 10 is arranged above the water surface of the seawater 3 in the breeding mechanism 1, the bottom of the collecting mechanism 10 is arranged below the water surface of the seawater 3 in the breeding mechanism 1, and the bottom of the collecting mechanism 10 is provided with a second filter screen 101.

[0090] The water pumping mechanism 11 is used to pump the seawater in the breeding mechanism 1 into the collecting mechanism 10.

[0091] When the amphioxus in the breeding mechanism 1 shows signs of mating behavior, the collecting mechanism 10 is arranged in the breeding mechanism 1, the second filter screen 101 is used to collect fertilized eggs, and the filtered seawater is returned to the breeding mechanism 1.

[0092] Specifically, the second filter screen 101 uses a 150-mesh silk screen.

[0093] The flow rate of the water pumping mechanism 11 is determined according to the fact that a continuous water flow is formed.

[0094] In some embodiments, the flow rate of each water pumping mechanism 11 is 0.3-0.4 L / min.

[0095] Example 2

[0096] The device described in Example 1 is used for the breeding experiment of the amphioxus parents.

[0097] Fertilized eggs are successfully obtained through the six breeding mechanisms, and the first-generation offspring is hatched, which indicates that the device provided by the present disclosure can enable the amphioxus parents to successfully perform mating behavior and reproduce the first-generation offspring.

[0098] The first-generation offspring obtained in each breeding mechanism is grouped and cultured, and each group of first-generation offspring corresponds to the parents obtained through the breeding of one breeding mechanism and is cultured to adulthood for about 11 months.

[0099] Each group of sub-generation respectively selects individual body length 35-55mm, active, strong sand drilling ability, gonad full of amphioxus, each two groups are paired, each group of pairing is put into a breeding mechanism 1 of the device described in embodiment 1, and the same method is used to preserve seeds, the sub-generation can also perform mating behavior, and the sub-generation is obtained by breeding, which shows that the sub-generation obtained by the device provided by the embodiment of the present disclosure is relatively robust, and can maintain the ability to reproduce and obtain the sub-generation.

[0100] The above only describes the preferred embodiments of the present disclosure and is not intended to limit the present disclosure. Those skilled in the art can make various modifications and changes to the present disclosure. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present disclosure shall be included in the protection scope of the present disclosure.

Claims

1. An amphioxus breeding and seedling collecting device, characterized in that, The device comprises: a breeding mechanism, a bottom of which is paved with sea sand, and the breeding mechanism is filled with seawater; a drainage port is arranged at the bottom of the breeding mechanism, and a sealing mechanism is arranged on the drainage port to seal the drainage port; a water inlet mechanism is arranged in the breeding mechanism, and the water inlet mechanism is provided with a water inlet port arranged below the water surface of the seawater in the breeding mechanism; a water outlet mechanism is arranged in the breeding mechanism, and the upper portion of the water outlet mechanism is provided with a water outlet port; a first filter screen is arranged at the bottom of the breeding mechanism, and the first filter screen is arranged outside the drainage port.

2. The apparatus of claim 1, wherein, The device further comprises a water storage mechanism in communication with the water inlet mechanism.

3. The apparatus of claim 2, wherein, The device is arranged in a breeding room.

4. The apparatus of claim 2, wherein, The water storage mechanism is in communication with a seawater pond.

5. The apparatus of claim 1, wherein, The water inlet mechanism is arranged along the circumference of the breeding mechanism, and the water inlet port is oriented in the same direction along the circumference of the breeding mechanism.

6. The apparatus of claim 1, wherein, The device further comprises an aeration mechanism.

7. The apparatus of claim 1, wherein, The water outlet mechanism is arranged on the sealing mechanism.

8. The apparatus of claim 1, wherein, The top of the first filter screen is in an open structure.

9. The apparatus of claim 1, wherein, The device further comprises: a collection mechanism arranged in the breeding mechanism, the top of the collection mechanism is arranged above the water surface of the seawater in the breeding mechanism, the bottom of the collection mechanism is arranged below the water surface of the seawater in the breeding mechanism, and the bottom of the collection mechanism is provided with a second filter screen; a water pumping mechanism for pumping the seawater in the breeding mechanism into the collection mechanism.

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