Novel water bath pot for cell resuscitation

Abstract

The utility model discloses a novel water bath pot for cell resuscitation. The novel water bath pot comprises a water bath pot box body, a heater, a shaking driver and a cryopreservation tube fixing assembly, the shaking driver comprises a motor, a speed reducer and a reciprocating shaking assembly, the motor drives the reciprocating shaking assembly to shake in a reciprocating mode through the speed reducer, and the speed reducer is used for speed reduction transmission between the motor and the reciprocating shaking assembly. The cryopreservation tube fixing assembly comprises a cryopreservation tube fixing plate and can be arranged in the water storage tank; the cryopreservation tube fixing plate is connected with a second hasp on the reciprocating shaking assembly in a buckled mode through the first hasp so that reciprocating shaking of the cryopreservation tube fixing plate can be achieved. The novel water bath pan for cell resuscitation has the advantages of shaking at a controlled speed, resuscitation of a large number of cells at one time and uniform heating.

Description

TECHNICAL FIELD

[0001] The utility model relates to a water bath, especially to a novel cell resuscitation water bath. BACKGROUND

[0002] In recent years, cell and gene therapy as a new generation of precision therapy, leads the biological pharmaceutical into a new round of tide. Among them, cell therapy refers to the application of human autologous or allogeneic cells after in vitro operation to input (or implant) into the human body, for the process of disease treatment, in vitro operation includes but is not limited to cell separation, expansion culture, cryopreservation and resuscitation.

[0003] In the research, transportation and application of cells, in order to ensure the performance of cells, it is usually cryopreserved, and then resuscitated after operation for subsequent research or clinical. The resuscitation operation involved, generally, researchers hold cell cryopreservation tubes, shake in the water bath with set temperature, and achieve cell resuscitation by observing the cell freeze-thaw state at the specified time or stage.

[0004] In this resuscitation process, due to the small volume of the water bath, the space occupied is small, and it is not convenient for multiple operations each time, and a single person can only resuscitate a limited number of cells, which is not convenient for large-scale cell resuscitation. Even if multiple people can operate, when each person judges the cell freeze-thaw state during resuscitation, if time is used as the basis for judgment, multiple timing instruments need to be provided, if the cell state is observed at stages as the basis for judgment, the influence on the water temperature when taking and placing the cells from the water bath each time cannot be judged on the influence on other cells being resuscitated; in addition, during the process of each person shaking the cryopreserved cells, the shaking amplitude, frequency and the height of placing the cell cryopreservation tube in the water bath (the amount of water the cryopreservation tube contacts, the heat received by the cells) are different, which will cause different cell melting times, that is, it is impossible to achieve true synchronization, which further affects the experimental progress and the influence of differences in the resuscitation process on the experimental results; furthermore, when resuscitating cells of different specifications, the water level of the water bath is judged by the researchers, there is no uniform standard, and the water level may be inconsistent due to different personnel, the water level is too low, which may cause the cell cryopreservation tube to not be fully immersed in the water bath, or to ensure that the cell cryopreservation tube can be fully heated, the risk of contamination is easily introduced by tilting the placement, the water level is too high, and when shaking the cryopreservation tube, the water in the water bath is easy to splash out, increasing the risk of environmental contamination; and, since it is a fully manual operation, the researchers cannot leave the water bath during the entire resuscitation process, which cannot be performed simultaneously with other experiments, and the labor cost is high; at the same time, the internal and external designs of the conventional cell resuscitation water bath are mostly cubes or cuboids, the four corners of the internal bottom are dead angles, and cleaning may not be complete, which greatly increases the risk of contamination or cross contamination. UTILITY MODEL CONTENTS

[0005] In order to solve at least one problem existing in the prior art described above, especially how to control the speed of shaking, perform a large number of cell resuscitation at one time and heat uniformly, the utility model provides a novel water bath kettle for cell resuscitation, which comprises:

[0006] A water bath kettle box body is internally provided with a water storage groove;

[0007] A heater is arranged in a sandwich layer formed by the bottom of the water storage groove and the bottom of the water bath kettle box body, and is used for heating the liquid in the water storage groove;

[0008] A shaking driver comprises a motor, a speed reducer and a reciprocating shaking assembly, the motor drives the reciprocating shaking assembly to reciprocate through the speed reducer, and the speed reducer is used for speed reduction transmission between the motor and the reciprocating shaking assembly;

[0009] A cryopreservation tube fixing assembly comprises a cryopreservation tube fixing plate and can be arranged in the water storage groove; the cryopreservation tube fixing plate is connected with a second buckle on the reciprocating shaking assembly through first buckles to realize reciprocating shaking of the cryopreservation tube fixing plate.

[0010] In some embodiments, the reciprocating shaking assembly comprises a first shaking member and a second shaking member; magnetic force transmission is realized between the first shaking member and the second shaking member through magnetic force connection; the first shaking member is connected with the speed reducer, and the second shaking member is provided with the second buckle.

[0011] In some embodiments, the reciprocating shaking assembly has a first section and a second section, a first end portion of the first section is connected with the second section, a second end portion of the first section is connected with the speed reducer, and a waterproof section higher than the highest water level of the water storage groove is arranged between the first end portion and the second end portion; the second buckle is located in the second section.

[0012] In some embodiments, a plurality of hole positions for placing cryopreservation tubes are arranged on the cryopreservation tube fixing plate; the cryopreservation tube fixing assembly further comprises a cryopreservation tube supporting bracket; a plurality of groups of connecting members are arranged on the vertical height of the cryopreservation tube supporting bracket, each group of the connecting members has different vertical heights, the cryopreservation tube fixing plate is erected on the cryopreservation tube supporting bracket through any one group of the connecting members, and the number of each group of the connecting members is greater than or equal to 2. The first buckle is arranged at the cryopreservation tube supporting bracket.

[0013] In some embodiments, the vertical heights of each group of the connecting members are the same, so that the cryopreservation tube fixing plate can be horizontally erected on the cryopreservation tube supporting bracket.

[0014] In some embodiments, the cross-section area of the reciprocating shaking component below the highest water level of the water storage tank in the reciprocating shaking direction is smaller than the longitudinal cross-section area of the reciprocating shaking component below the highest water level of the water storage tank in the reciprocating shaking direction.

[0015] In some embodiments, the part of the reciprocating shaking component below the highest water level of the water storage tank is in the shape of a sheet and / or an elliptic cylinder.

[0016] In some embodiments, the bottom corners of the water storage tank are arc-shaped curved surfaces, which have the advantage of easy cleaning.

[0017] In some embodiments, the sidewall of the water storage tank is provided with a water level indicator and can be observed from the outside of the new water bath for cell recovery.

[0018] In some embodiments, a control panel is further included; the control panel is in signal connection with the heater and the shaking driver, and has a timer and an operation end; the control panel is used for controlling the heating temperature, the reciprocating shaking rate and the cell recovery time; the operation end is used for realizing the above control; the operation end is one or more of a knob, a button and a touch display screen.

[0019] In some embodiments, the control panel further has an alarm for sending an alarm signal after the cell recovery time reaches the set time; the alarm signal is an acoustic signal or / and an optical signal.

[0020] In some embodiments, a water bath cover is further included, and the water bath cover is provided with a handle; a drain is arranged below the lowest water level of the water storage tank.

[0021] The beneficial effects are:

[0022] The new water bath for cell recovery has the advantages of speed-controlled shaking, one-time large-scale cell recovery and uniform heating. The size difference of the hole positions on the cryopreservation tube fixing plate can be used for different specifications of cryopreservation tubes. The multiple groups of connecting pieces with different vertical heights are conducive to setting the cryopreservation tube fixing plate loaded with cryopreservation tubes at different water levels and are more conducive to one-time large-scale cell recovery. The control panel and the like are more conducive to temperature control, speed-controlled shaking and timing. Some embodiments of the utility model can also meet the cell recovery of different specifications of cryopreservation, and have the characteristics of easy cleaning.

[0023] The concept, specific structure and generated technical effects of the utility model will be further described below with reference to the drawings, so as to fully understand the purpose, characteristics and effects of the utility model. BRIEF DESCRIPTION OF DRAWINGS

[0024] In order to more clearly illustrate the technical scheme of the embodiments of the present application, the drawings needed to be used in the embodiments or prior art description will be briefly introduced as follows. Obviously, the drawings described below are only some embodiments of the present application, and for those skilled in the art, other drawings can be obtained without creative labor on the premise of the drawings.

[0025] Figure 1 Figure 1 is a structural schematic diagram of the novel cell recovery water bath in a cover-opened state according to an embodiment of the present application.

[0026] Figure 2 Figure 2 is a top perspective view of the novel cell recovery water bath in a cover-opened state according to an embodiment of the present application.

[0027] Figure 3 Figure 3 is a sectional view along AA. Figure 2

[0028] Figure 4 Figure 4 is a schematic diagram of the overall structure according to an embodiment of the present application.

[0029] Explanation of reference signs:

[0030] 1 - water bath box, 11 - water bath cover, 111 - handle, 12 - water storage tank, 121 - drain, 122 - arc-shaped curved surface,

[0031] 2 - heater,

[0032] 3 - shaking driver, 31 - reciprocating shaking assembly, 311 - first section, 312 - second section, 313 - second buckle,

[0033] 4 - cryopreservation tube fixing assembly, 41 - cryopreservation tube fixing plate, 42 - cryopreservation tube supporting bracket, 422 - connecting piece, 43 - first buckle,

[0034] 5 - control panel,

[0035] 6 - power cord. DETAILED DESCRIPTION

[0036] In order to make the purpose, technical scheme and advantages of the embodiments of the present application more clear, the technical scheme in the embodiments of the present application will be described clearly and completely in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only some embodiments of the present application, not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor belong to the scope of protection of the present application.

[0037] ​The terms "first", "second", etc. are used only for the purpose of description and do not indicate or imply relative importance or a specific number of the technical features indicated. Thus, the features defined with "first", "second" can explicitly or implicitly include at least one of the features. In the description of the present application, if the term "a plurality of" appears, the meaning of "a plurality of" is at least two, such as two, three, etc., unless otherwise explicitly specified and limited.

[0038] In the present application, unless otherwise explicitly specified and limited, if the terms "mounting", "connecting", "connecting", "fixing" and the like appear, these terms should be interpreted broadly. For example, it can be fixedly connected, or it can be detachably connected, or it can be integrated; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the internal communication of two elements or the interaction relationship between two elements, unless otherwise explicitly limited. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0039] If an element is referred to as "fixed to" or "disposed on" another element, it can be directly on another element or there can be a middle element. If an element is considered to be "connected" to another element, it can be directly connected to another element or there can be a middle element. If present, the terms "vertical", "horizontal", "up", "down", "left", "right" and similar expressions used in the present application are for illustrative purposes only and do not represent the only implementation.

[0040] An embodiment of the present application provides a novel water bath for cell recovery (such as Figures 1-4 As shown in the figure), comprising: a water bath box body 1, a water bath cover 11, a heater 2, a shaking driver 3, a frozen tube fixing assembly 4 and a control panel 5.

[0041] The water bath box body 1 is provided with a water storage tank 12. The water bath cover 11 is provided with a handle 111; the water storage tank 12 is provided with a drain 121 below the lowest water level line.

[0042] The heater 2 is arranged in the interlayer formed by the bottom of the water storage tank 12 and the bottom of the water bath box body 1, and is used for heating the liquid in the water storage tank 12.

[0043] The shaking driver 3 includes a motor, a speed reducer and a reciprocating shaking assembly 31, the motor drives the reciprocating shaking assembly 31 to reciprocate through the speed reducer, and the speed reducer is used for speed reduction transmission between the motor and the reciprocating shaking assembly 31.

[0044] The freezing tube fixing assembly 4 comprises a freezing tube fixing plate 41, which can be arranged in the water storage tank 12; the freezing tube fixing plate 41 is connected with the second buckle 313 on the reciprocating shaking assembly 31 through the first buckle 43, so that the reciprocating shaking of the freezing tube fixing plate 41 can be realized. The freezing tube fixing plate 41 is provided with a plurality of hole positions for placing freezing tubes; the freezing tube fixing assembly 4 further comprises a freezing tube supporting bracket 42; a plurality of connecting members 422 are arranged on the vertical height of the freezing tube supporting bracket 42, and each group of connecting members 422 has different vertical heights; the freezing tube fixing plate 41 is arranged on the freezing tube supporting bracket 42 through any one group of connecting members 422; and the number of each group of connecting members 422 is greater than or equal to 2. The vertical heights of each group of connecting members 422 are the same, so that the freezing tube fixing plate 41 can be horizontally arranged on the freezing tube supporting bracket 42, thereby the freezing tube fixing plate 41 can be arranged at different vertical heights according to the size of the freezing tube or the need.

[0045] The reciprocating shaking assembly 31 has a first section 311 and a second section 312; the first end of the first section 311 is connected with the second section 312, the second end of the first section 311 is connected with a speed reducer, and a waterproof section higher than the highest water level of the water storage tank 12 is arranged between the first end and the second end; and the second buckle 313 is located on the second section 312.

[0046] The bottom corners of the water storage tank 12 are arc-shaped curved surfaces 122, which have the advantage of easy cleaning. The sidewall of the water storage tank 12 is provided with a water level indicating mark, which can be observed from the outside of the new water bath for cell recovery.

[0047] The cross-sectional area of the part of the reciprocating shaking assembly 31 extending below the highest water level line of the water storage tank 12 in the transverse direction of the reciprocating shaking is smaller than the cross-sectional area in the longitudinal direction of the reciprocating shaking, so that the water body shaking caused by the reciprocating shaking can be reduced as much as possible, and the resistance of the water to the reciprocating shaking assembly 31 can also be reduced. The part of the reciprocating shaking assembly 31 extending below the highest water level line of the water storage tank 12 is in the shape of a sheet and / or an elliptical cylinder.

[0048] The control panel 5 is signal connected with the heater 2 and the shaking driver 3, and is used for controlling the heating temperature and the reciprocating shaking rate. The control panel 5 has a timer, an operation end and an alarm. The control panel 5 reads the time feedback by the timer, and is used for controlling and setting the cell recovery time. The operation end is used for realizing the control of the heating temperature, the reciprocating shaking rate and the cell recovery time. The operation end is one or more of a knob, a button and a touch display screen. The alarm is used for sending an alarm signal after the cell recovery time reaches the set time; and the alarm signal is a sound signal or / and an optical signal.

[0049] The new cell recovery water bath all the components that need electricity are powered by power cord 6, or part of the power supply line is powered by part of the power supply line is powered by the built-in power supply. The power supply mode of the utility model is the conventional prior art, which will not be described here. The shaking driver is similar to the shaking driver of the existing biological culture shaking bed equipment. The heater uses a conventional water bath heater with a heating coil.

[0050] In some embodiments, the reciprocating shaking assembly comprises a first shaking member and a second shaking member; the first shaking member and the second shaking member are connected by magnetic force to realize magnetic transmission; the first shaking member is connected with the speed reducer, and the second shaking member is provided with the second buckle. The first shaking member and the second shaking member of the shaking driver in this embodiment are connected by magnetic force (similar to a magnetic coupler) to isolate the liquid in the water storage tank from the motor and the speed reducer, preventing liquid leakage and improving the safety and reliability of the new cell recovery water bath.

[0051] The technical features of the above embodiments can be combined in any way. To make the description concise, all possible combinations of the technical features in the above embodiments are not described, but as long as the combination of the technical features does not exist contradictory, it should be considered as the scope of the present disclosure.

[0052] The above embodiments only express several embodiments of the present application, and the description is more specific and detailed, but it should not be understood as a limitation on the scope of the patent. It should be noted that for ordinary skilled in the art, without departing from the concept of the present application, a number of variations and improvements can be made, which are within the scope of the present application.

Claims

1. A new type of cell recovery water bath, characterized in that, The utility model relates to a novel cell recovery water bath, which comprises: a water bath box body provided with a water storage tank inside; a heater arranged in the interlayer formed by the bottom of the water storage tank and the bottom of the water bath box body, used for heating the liquid in the water storage tank; a shaking driver, which comprises a motor, a speed reducer and a reciprocating shaking assembly, the motor drives the reciprocating shaking assembly to reciprocate through the speed reducer, and the speed reducer is used for speed reduction transmission between the motor and the reciprocating shaking assembly; a cryogenic tube fixing assembly, which comprises a cryogenic tube fixing plate and can be arranged in the water storage tank; the cryogenic tube fixing plate is connected with the second buckle on the reciprocating shaking assembly through the first buckle to realize reciprocating shaking of the cryogenic tube fixing plate.

2. The new type of water bath for cell recovery as claimed in claim 1, wherein, The reciprocating shaking assembly comprises a first shaking member and a second shaking member; the first shaking member and the second shaking member are connected through magnetic force to realize magnetic transmission; the first shaking member is connected with the speed reducer, and the second shaking member is provided with the second buckle.

3. The new type of water bath for cell recovery as claimed in claim 1, wherein, The reciprocating shaking assembly has a first section and a second section; the first end of the first section is connected with the second section, the second end of the first section is connected with the speed reducer, and a waterproof section higher than the highest water level of the water storage tank is arranged between the first end and the second end; the second buckle is located in the second section.

4. The new type of water bath for cell recovery of claim 3, characterized in that, The area of the cross section of the part of the reciprocating shaking assembly extending below the highest water level line of the water storage tank in the reciprocating shaking direction is smaller than the area of the longitudinal section in the reciprocating shaking direction.

5. The new type of water bath for cell recovery as claimed in claim 1, wherein, The cryogenic tube fixing plate is provided with a plurality of hole positions for placing cryogenic tubes; the cryogenic tube fixing assembly further comprises a cryogenic tube supporting bracket; a plurality of groups of connecting members are arranged on the vertical height of the cryogenic tube supporting bracket, each group of connecting members has different vertical heights, the cryogenic tube fixing plate is arranged on the cryogenic tube supporting bracket through any group of connecting members, and the number of each group of connecting members is greater than or equal to 2.

6. The novel water bath for cell recovery of claim 5, wherein, The vertical heights of each group of connecting members are the same, so that the cryogenic tube fixing plate can be horizontally arranged on the cryogenic tube supporting bracket.

7. The new type of water bath for cell recovery as claimed in claim 1, wherein, The four corners of the bottom of the water storage tank are arc-shaped curved surfaces.

8. The new type of water bath for cell recovery of claim 1, wherein, The sidewall of the water storage tank is provided with a water level indicating mark and can be observed from the outside of the novel cell recovery water bath.

9. The new type of water bath for cell recovery as claimed in claim 1, wherein, The control panel is signal-connected with the heater and the shaking driver, has a timer and an operation end, and is used for controlling the heating temperature, the reciprocating shaking rate and the cell recovery time; the operation end is used for realizing the above control; the operation end is one or more of a knob, a button and a touch display screen.

10. The novel water bath for cell recovery of claim 9, wherein, The control panel further has an alarm for sending an alarm signal when the cell recovery time reaches the set time; the alarm signal is a sound signal or / and an optical signal.

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