Nucleic acid extraction tube

Abstract

The utility model relates to the technical field of nucleic acid extraction, and discloses a nucleic acid extraction tube which comprises a tube body, a first sealing ring enhances the sealing performance between a sealing cover and the tube body and effectively prevents liquid leakage and external pollution, and a label provides an identification area of sample information. And sample tracking and management in the experiment process are facilitated. According to the nucleic acid extraction tube, the tube body provides a stable storage and operation space, the gradually-reduced structure of the tail tube enables liquid to smoothly flow to an elution tank, the silica gel membrane provides efficient nucleic acid adsorption performance, the filter membrane effectively blocks impurity particles, and the supporting net provides stable support for the silica gel membrane and the filter membrane, so that deformation or damage in the operation process is prevented; the elution tank serves as a final nucleic acid sample collection area, concentrated storage and efficient recovery of nucleic acid samples are guaranteed, the parts are matched with one another to form an efficient nucleic acid extraction structure, the purity and the recovery rate of nucleic acid extraction are improved, and sample loss is reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to nucleic acid extraction technical field, concretely is a nucleic acid extraction tube. BACKGROUND

[0002] Nucleic acid extraction tube is a kind of experimental tool for extracting DNA or RNA from biological sample, is widely used in scientific research laboratory, clinical diagnosis, gene detection and biomedical research field, its main function is through a series of physical and chemical means, the nucleic acid molecule (DNA or RNA) in cell or tissue sample is efficiently separated and purified, the purity and integrity of nucleic acid have direct influence on subsequent PCR amplification, gene sequencing, molecular cloning etc., is the indispensable important link in molecular biology experiment.

[0003] Traditional nucleic acid extraction method often relies on manual operation, needs multiple centrifugation, precipitation, washing etc., operation is tedious and time-consuming is longer, in addition, traditional method is easy to introduce cross contamination between different steps, leading to the purity of extracted nucleic acid sample to decline, influence the accuracy of experimental results, simultaneously, due to the limitation of equipment and operation, traditional extraction method has obvious deficiency in sample recovery rate, repeatability and automation degree, nucleic acid extraction process needs repeatedly transfer sample, increases the possibility of sample loss, this not only reduces the efficiency of nucleic acid extraction, also improves the time cost and manpower cost of experiment. UTILITY MODEL CONTENT

[0004] (I) the technical problem solved

[0005] The utility model aims at providing a nucleic acid extraction tube to solve the problem of cross contamination between different steps of traditional nucleic acid extraction tube proposed in the above background.

[0006] (II) technical scheme

[0007] In order to achieve the above object, the utility model provides the following technical scheme: a nucleic acid extraction tube, the nucleic acid extraction tube includes the pipe body, the upper side fixed setting of pipe body is provided with the sealing cover, the outer ring fixed setting of sealing cover is provided with the antiskid line, the top fixed setting of pipe body is provided with the convex ring, the inside of convex ring is provided with the sample inlet, the inside fixed setting of sealing cover is provided with the first sealing ring, the outer ring fixed setting of pipe body is provided with the label, and the pipe body is used as the main structure of nucleic acid extraction tube, is used to store sample, lysate, washing liquid and eluent, provides the space of liquid flow and reaction in the nucleic acid extraction process, and the sealing cover is used for sealing whole pipe body, prevents sample and reagent leakage, ensures the airtightness of internal environment in the extraction process, and the antiskid line increases the friction when operating, and it is more stable when the experimental personnel twists open or closes the sealing cover, and the convex ring plays the role of fixing and supporting, and provides structural support for the addition of sample and reagent, and the sample inlet is used as the channel of sample and reagent, makes liquid accurate addition in the pipe body, and the first sealing ring strengthens the sealing between sealing cover and pipe body, effectively prevents liquid leakage and external pollution, and the label provides the identification area of sample information, facilitates sample tracking and management in the experimental process.

[0008] Preferably, the lower end of the pipe body is fixedly provided with a tail pipe, the tail pipe gradually reduces in cross-sectional diameter from top to bottom, and the tail pipe forms a gradually reduced channel structure, so that the liquid can flow more concentratedly to the lower side.

[0009] Preferably, a silica gel membrane is fixedly arranged at an upper position in the pipe body, and a filter membrane is fixedly arranged at the lower side of the silica gel membrane.

[0010] Preferably, support nets are fixedly arranged at the lower ends of the silica gel membrane and the filter membrane, and the outer rings of the support nets are fixedly arranged on the inner walls of the pipe body.

[0011] Preferably, a rotating part is fixedly arranged at the upper end of the tail pipe, and an outer thread is fixedly arranged at the outer ring of the rotating part.

[0012] Preferably, an inner thread is fixedly arranged at the bottom of the pipe body, and the outer thread is embedded in the inner thread.

[0013] Preferably, a second sealing ring is fixedly arranged between the inner walls of the pipe body and the rotating part, and an elution groove is fixedly arranged in the tail pipe.

[0014] Compared with the prior art, the utility model has the beneficial effects that:

[0015] 1、the nucleic acid extraction tube, the pipe body provides stable storage and operation space, the sealing cover effectively prevents sample leakage and external pollution, the anti-skid pattern increases the stability during operation, the sample inlet enables the sample and reagent to be conveniently added into the pipe, the first sealing ring and the second sealing ring jointly ensure the airtightness of the extraction process, avoid liquid leakage, the gradually narrowing structure of the tail pipe enables liquid to smoothly flow to the elution groove, the silica gel membrane provides high-efficiency nucleic acid adsorption performance, the filter membrane effectively blocks impurity particles, the supporting net provides stable support for the silica gel membrane and the filter membrane, prevents deformation or damage during operation, the elution groove is used as the collection area of the final nucleic acid sample, guarantees the centralized storage and high-efficiency recovery of the nucleic acid sample, the mutual cooperation between these parts forms a high-efficiency nucleic acid extraction structure, improves the purity and recovery rate of nucleic acid extraction, and reduces sample loss;

[0016] 2、the nucleic acid extraction tube, the anti-skid pattern of the sealing cover increases the convenience of operation, avoids operation errors caused by hand slipping, the sample inlet enables the addition of the sample and reagent to be more accurate, the label design of the pipe body facilitates sample identification and management of experimenters, the combined design of the rotating part and the external thread and the internal thread enables the tail pipe to be quickly disassembled, the elution groove is reasonably designed, so that nucleic acid solution can be collected in a centralized manner, subsequent recovery is facilitated, the whole extraction process is clear in steps and smooth in operation, effectively reduces the error rate in the manual operation process, simultaneously reduces operation time cost, improves the efficiency and repeatability of nucleic acid extraction;

[0017] 3、the nucleic acid extraction tube, the nucleic acid extraction tube ensures that the whole extraction process is carried out in a closed environment through a multiple sealing structure, the first sealing ring and the second sealing ring effectively prevent liquid from leaking during the extraction process, the external thread of the tail pipe is combined with the internal thread of the pipe body, the combination ensures stable connection, the combination of the supporting net and the supporting gasket provides physical stability, prevents the silica gel membrane and the filter membrane from being displaced or damaged under the condition of centrifugation or high pressure, the effective filtration of the filter membrane reduces the interference of impurities, ensures the purity of the nucleic acid sample, the reasonable design of the elution groove prevents cross contamination and leakage of the nucleic acid sample, simultaneously, all parts are precisely machined, so that each part can stably operate in the working process, these safety and stability designs enable the nucleic acid extraction tube to exhibit excellent reliability and consistency under different environments and experimental conditions. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 it is the three-dimensional structure schematic view of nucleic acid extraction tube of the utility model;

[0019] Figure 2 it is the explosion drawing of nucleic acid extraction tube of the utility model;

[0020] Figure 3 it is the sealing cover structure schematic view of the utility model;

[0021] Figure 4 It is the profile structure schematic view of the nucleic acid extraction tube of the utility model.

[0022] Figure 5 It is the profile structure schematic view of the tail pipe of the utility model.

[0023] In the drawing: 1, pipe body;2, sealing cover;3, anti-skid pattern;4, convex ring;5, sample inlet;6, first sealing ring;7, label;8, tail pipe;9, silica gel film;10, filter membrane;11, support net;12, rotating part;13, external thread;14, internal thread;15, second sealing ring;16, elution groove. DETAILED DESCRIPTION

[0024] The technical scheme in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0025] Please refer to Figures 1-5 The utility model provides a technical scheme: a nucleic acid extraction tube, the nucleic acid extraction tube includes pipe body 1, the upside of pipe body 1 is fixedly provided with sealing cover 2, the outer ring of sealing cover 2 is fixedly provided with anti-skid pattern 3, the top of pipe body 1 is fixedly provided with convex ring 4, the inside of convex ring 4 is provided with sample inlet 5, the inside of sealing cover 2 is fixedly provided with first sealing ring 6, the outer ring of pipe body 1 is fixedly provided with label 7, pipe body 1 is as the main structure of nucleic acid extraction tube, provides the storage space of sample, reagent and nucleic acid solution, ensures that liquid is stable in the inside flow, sealing cover 2 is used for sealing pipe body, prevents sample and reagent from leaking, ensures that the internal environment is airtight, avoids the entry of external pollution, anti-skid pattern 3 increases the friction when operating, so that when the sealing cover is screwed open or tight, it is more stable, convex ring 4 is as a structural support component, helps liquid to be evenly distributed in the inside of pipe body, sample inlet 5 is as the flow channel of sample and reagent, ensures that liquid can accurately enter the inside of pipe body, first sealing ring 6 provides sealing performance, prevents liquid from leaking during operation, ensures the stable environment in the inside of pipe body, label 7 is as an identification area, is used for recording sample information, facilitates the sample identification and tracking of experimental personnel.

[0026] The lower end of the pipe body 1 is fixedly provided with a tail pipe 8, the tail pipe 8 is gradually reduced in diameter from top to bottom, the inside of the pipe body 1 is fixedly provided with a silica gel membrane 9 at the upper position, the lower side of the silica gel membrane 9 is fixedly provided with a filter membrane 10, the tail pipe 8 serves as a transition channel for liquid flow, allowing the liquid to gradually concentrate during the process, reducing sample residue, the silica gel membrane 9 serves as a core functional component, selectively adsorbing nucleic acid molecules under specific pH and ionic strength conditions, effectively separating nucleic acids and other impurities, the filter membrane 10 performs preliminary filtration on the liquid, removing large-particle impurities and cell fragments, ensuring the purity of the liquid.

[0027] The lower end of the silica gel membrane 9 and the filter membrane 10 is fixedly provided with a support net 11, the outer ring of the support net 11 is fixedly provided on the inner wall of the pipe body 1, the upper end of the tail pipe 8 is fixedly provided with a rotating part 12, the outer ring of the rotating part 12 is fixedly provided with an external thread 13, the bottom of the pipe body 1 is fixedly provided with an internal thread 14, the external thread 13 is embedded with the internal thread 14, the inner wall between the pipe body 1 and the rotating part 12 is fixedly provided with a second sealing ring 15, the inside of the tail pipe 8 is fixedly provided with an elution groove 16, the rotating part 12 is connected and detached with the pipe body through rotation operation, providing convenience for operation, the external thread 13 is embedded with the internal thread 14, ensuring the stable connection and sealing between the rotating part and the pipe body, preventing liquid leakage during operation, the second sealing ring 15 serves as an auxiliary sealing structure, further enhancing the sealing performance of the connection part, preventing liquid from leaking into the external environment, the support net 11 provides physical support for the silica gel membrane and the filter membrane, preventing them from deforming or breaking during liquid flow or centrifugation, ensuring the stability of nucleic acid extraction, the elution groove 16 serves as a collection area for the final nucleic acid solution, used for storing the nucleic acid solution eluted from the silica gel membrane, ensuring the concentrated collection of samples and facilitating subsequent use.

[0028] Working principle: first, hold the edge of the sealing cover 2 and rotate it off, exposing the sample inlet 5, add the nucleic acid sample to be extracted and the lysis solution into the pipe body 1 through the sample inlet 5, the sample liquid flows to the silica gel membrane 9 under the action of gravity, the silica gel membrane 9 selectively adsorbs nucleic acid molecules under specific pH and ionic conditions, other impurities flow to the filter membrane 10, the filter membrane 10 performs secondary filtration on the liquid, removing small impurities that are not trapped, the support net 11 provides physical support for the silica gel membrane 9 and the filter membrane 10, ensuring that both remain stable during liquid passage, then, use washing liquid to clean the silica gel membrane 9, remove residual protein and salt, avoid affecting the purity of nucleic acid, then add elution liquid to release the nucleic acid adsorbed on the silica gel membrane 9, the nucleic acid solution passes through the filter membrane 10 and the support net 11, and finally flows into the elution groove 16 inside the tail pipe 8, at this time, hold the tail pipe 8 and rotate it to rotate the rotating part 12, the external thread 13 gradually separates from the internal thread 14, the tail pipe 8 is detached, the purified nucleic acid solution in the elution groove 16 is taken out, the whole extraction process is completed, and high-purity nucleic acid solution is obtained.

[0029] It should be pointed out finally that the above is only to illustrate the technical solutions of the present application, and is not a limit on the scope of protection of the present application, and simple modifications or equivalent replacements of the technical solutions of the present application by those skilled in the art do not deviate from the essence and scope of the technical solutions of the present application.

Claims

1. A nucleic acid extraction tube comprising a tube body (1), characterized in that: The upper side of the pipe body (1) is fixedly provided with a sealing cover (2), the outer ring of the sealing cover (2) is fixedly provided with an anti-skid pattern (3), the top end of the pipe body (1) is fixedly provided with a convex ring (4), the inside of the convex ring (4) is provided with a sample inlet (5), the inside of the sealing cover (2) is fixedly provided with a first sealing ring (6), and the outer ring of the pipe body (1) is fixedly provided with a label (7).

2. The nucleic acid extraction tube of claim 1, wherein: The lower end of the pipe body (1) is fixedly provided with a tail pipe (8), and the cross-sectional diameter of the tail pipe (8) gradually decreases from top to bottom.

3. The nucleic acid extraction tube of claim 2, wherein: The inside of the pipe body (1) is fixedly provided with a silica gel membrane (9) at an upper position, and the lower side of the silica gel membrane (9) is fixedly provided with a filter membrane (10).

4. The nucleic acid extraction tube of claim 3, wherein: The lower end of the silica gel membrane (9) and the filter membrane (10) are both fixedly provided with a support net (11), and the outer ring of the support net (11) is fixedly arranged on the inner wall of the pipe body (1).

5. The nucleic acid extraction tube of claim 2, wherein: The upper end of the tail pipe (8) is fixedly provided with a rotating part (12), and the outer ring of the rotating part (12) is fixedly provided with an external thread (13).

6. The nucleic acid extraction tube of claim 1, wherein: The bottom of the pipe body (1) is fixedly provided with an internal thread (14), and the external thread (13) is embedded with the internal thread (14).

7. The nucleic acid extraction tube of claim 2, wherein: The inner wall between the pipe body (1) and the rotating part (12) is fixedly provided with a second sealing ring (15), and the inside of the tail pipe (8) is fixedly provided with an elution groove (16).

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