Stainless steel rotating device for reducing mycorrhizal fungus infection of plant root system
By using a rotating device made of stainless steel and welded copper rods, the problems of high-temperature sterilization and contamination of nylon and plastic devices were solved, achieving a high efficiency reduction in mycorrhizal fungal infection and ensuring the accuracy and reliability of experiments.
Patent Information
- Application Number
- CN202520218337.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-12
- Publication Date
- 2026-01-06
- Estimated Expiration
- 2035-02-12
AI Technical Summary
In existing technologies, rotating devices made of nylon and plastic cannot be sterilized at high temperatures, resulting in reduced continuity of the soil interface inside and outside the device, soil contamination with glue, and soil easy to adhere to the filter surface, affecting the accuracy and reliability of the experiment.
The frustum-shaped and cylindrical filter screens are made of stainless steel, combined with copper rod welding and stainless steel ring design to avoid chemical adhesive contamination, ensure that the device can withstand high-temperature sterilization, and reduce root mycorrhizal fungal infection through rotation.
It achieves high-temperature sterilization without pollution, maintains soil continuity, significantly reduces fungal infection, is simple to operate and low in cost, and is suitable for long-term use.
Smart Images
Figure CN223758866U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the interdisciplinary field of agriculture, forestry and biotechnology, and in particular to a stainless steel rotating device for reducing mycorrhizal fungal infection of plant roots. Background Technology
[0002] In most terrestrial plants, the root system forms a symbiotic relationship with mycorrhizal fungi. Exploring the response and mechanisms of root systems to environmental changes is an important field. However, in the field, it is difficult to distinguish whether this response is contributed by the roots or the symbiotic mycorrhizal fungi. This makes accurately measuring the physiological and molecular processes of roots and mycorrhizal fungi quite challenging. Because the basic respiration processes of roots and mycorrhizal fungi are similar, when differences exist in root respiration, it is difficult to identify whether the difference is caused by changes in root respiration or by mycorrhizal fungal activity.
[0003] The common approach is to construct a device that significantly reduces or even eliminates mycorrhizal fungal infection of the target root system, creating a control root system with "few" or "no" mycorrhizal fungal symbiosis. This allows for comparison with normal root systems to reveal the root system's environmental response and mechanisms. Traditional methods typically use nylon and PVC pipes, but these have several shortcomings in mycelial rotation separation experiments:
[0004] 1. Nylon and plastic materials cannot be sterilized in autoclaves with high pressure and high temperature;
[0005] 2. If the proportion of PVC pipes in the equipment area is too large, it will reduce the continuity of the soil interface between the inside and outside of the equipment;
[0006] 3. Nylon filters and PVC plastic frames are often connected with glue. Glue may pollute the soil and directly or indirectly interfere with plant root growth, affecting the accuracy and reliability of the experiment.
[0007] 4. Soil easily adheres to the surface of the nylon filter, resulting in poor removal of fungal infections.
[0008] Therefore, there is an urgent need for a simple, effective, and minimally damaging method to remove root-symbiotic fungi and construct control root systems. Utility Model Content
[0009] The purpose of this utility model embodiment is to provide a stainless steel rotating device for reducing the infection of root mycorrhizal fungi in plants. It reduces the infection of root symbiotic mycorrhizal fungi through physical rotation, and solves the problems of traditional "rotating device" applications, such as inability to sterilize at high temperature, blockage of soil moisture and nutrient exchange, soil particles adhering to the filter screen, and chemical pollution of the soil.
[0010] To solve the above-mentioned technical problems, the technical solution adopted by this utility model is as follows:
[0011] This utility model embodiment provides a stainless steel rotating device for reducing mycorrhizal fungal infection of plant roots, comprising:
[0012] Frustum-shaped filter screen, cylindrical filter screen, handle and rivet;
[0013] The bottom of the frustum-shaped filter screen is connected to the top of the cylindrical filter screen and the interiors are interconnected. The top of the frustum-shaped filter screen is provided with an opening to facilitate the introduction of the target root system.
[0014] The handle is located at the connection between the frustum-shaped filter screen and the cylindrical filter screen;
[0015] The frustum-shaped filter screen has a flap on its side, and round holes are provided on both sides of the flap. After the target root system is introduced, the rivet passes through the round holes to fix the flap on the side of the frustum-shaped filter screen.
[0016] In some embodiments, a stainless steel ring is provided at the connection between the frustum-shaped filter screen and the cylindrical filter screen.
[0017] In some embodiments, the handle is disposed on the stainless steel ring.
[0018] In some embodiments, both the frustum-shaped filter and the cylindrical filter are made of stainless steel and have a pore size of 30-40 μm.
[0019] In some embodiments, the connections between the frustum-shaped filter, the cylindrical filter, and the handle are all welded with copper rods.
[0020] In some embodiments, the handle is made of stainless steel.
[0021] The stainless steel rotating device provided by this utility model for reducing mycorrhizal fungal infection of plant roots has the following advantages:
[0022] 1. Stainless steel filter screens are heat-resistant and can be sterilized at high temperatures without affecting their chemical and physical properties, thus reducing the risk of microbial contamination;
[0023] 2. The stainless steel filter screen itself is very rigid, and only stainless steel rings are needed for support. This design increases the area ratio of the filter screen, which is conducive to the interaction of water and nutrients in the soil inside and outside the device and has little impact on the continuity of the soil.
[0024] 3. Use copper rod spot welding technology to integrate the device, avoiding chemical adhesive contamination;
[0025] 4. This device is an integrated design made of 304 stainless steel, which has high chemical stability and is suitable for long-term burial in the soil without causing soil particles to adhere to the filter screen surface.
[0026] Furthermore, the device provided by this invention, through its rotating mechanism, can significantly reduce the infection of root mycorrhizal fungi within the filter screen. This device is low in cost, simple to operate, and highly effective. Attached Figure Description
[0027] To more clearly illustrate the technical solutions in this disclosure, the accompanying drawings used in some embodiments of this disclosure will be briefly described below. Obviously, the drawings described below are only drawings of some embodiments of this disclosure, and those skilled in the art can obtain other drawings based on these drawings. In addition, the drawings described below can be regarded as schematic diagrams and are not intended to limit the actual size of the product, the actual process of the method, etc. involved in the embodiments of this disclosure.
[0028] Figure 1 This is a schematic diagram of the structure of a stainless steel rotating device according to some embodiments of the present disclosure;
[0029] Figure 2 This is a schematic diagram of the structure of a frustum-shaped filter screen according to some embodiments of the present disclosure;
[0030] Figure 3 This is a schematic diagram illustrating the effect of rotation treatment on mycorrhizal infection rate according to some embodiments of this disclosure. Detailed Implementation
[0031] The technical solutions in some embodiments of this disclosure will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this disclosure, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments provided in this disclosure are within the scope of protection of this disclosure.
[0032] Unless the context otherwise requires, throughout the specification and claims, the term "comprising" is interpreted as open-ended and encompassing, meaning "including, but not limited to." In the description of the specification, terms such as "one embodiment," "some embodiments," "exemplary embodiment," "example," or "some examples" are intended to indicate that a particular feature, structure, material, or characteristic associated with that embodiment or example is included in at least one embodiment or example of this disclosure. The illustrative representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics mentioned may be included in any suitable manner in any one or more embodiments or examples.
[0033] This utility model embodiment provides a stainless steel rotating device for reducing mycorrhizal fungal infection of plant roots, such as... Figures 1 to 2 As shown, it includes: a frustum-shaped filter screen 1, a cylindrical filter screen 2, a handle 3, and a rivet 4.
[0034] In some embodiments, the bottom of the frustum-shaped filter 1 is connected to the top of the cylindrical filter 2 and the interiors are interconnected. The top of the frustum-shaped filter 1 is provided with an opening to facilitate the introduction of the target root system.
[0035] For example, the bottom of the frustum-shaped filter 1 is welded to the top of the cylindrical filter 2 using copper rods. This avoids the use of lead rods for welding, thus preventing soil and plant contamination.
[0036] In some embodiments, a handle 3 is disposed at the connection between the frustum-shaped filter 1 and the cylindrical filter 2. The handle 3 allows for easy rotation of the device, thereby reducing the infection of root symbiotic mycorrhizal fungi.
[0037] In some embodiments, a flap 7 is provided on the side of the frustum-shaped filter 1, and round holes 5 are provided on both sides of the flap 7. After the target root system is introduced, the flap 7 on the side of the frustum-shaped filter 1 is fixed by passing through the round holes 5 with rivets 4.
[0038] It should be noted that the present invention does not limit the number of circular holes 5, which can be set according to specific usage requirements. Preferably, four circular holes 5 with a diameter of 4mm are provided on each of the left and right sides of the placket 7.
[0039] For example, rivet 4 is a large-cap three-prong lantern rivet 4.
[0040] The stainless steel rotating device provided by this utility model for reducing mycorrhizal fungal infection of plant roots has the following advantages:
[0041] 1. Stainless steel filter screens are heat-resistant and can be sterilized at high temperatures without affecting their chemical and physical properties, thus reducing the risk of microbial contamination;
[0042] 2. The stainless steel filter screen itself is very rigid, and only stainless steel rings are needed for support. This design increases the area ratio of the filter screen, which is conducive to the interaction of water and nutrients in the soil inside and outside the device and has little impact on the continuity of the soil.
[0043] 3. Use copper rod spot welding technology to integrate the device, avoiding chemical adhesive contamination;
[0044] 4. This device is an integrated design made of 304 stainless steel, which has high chemical stability and is suitable for long-term burial in the soil without causing soil particles to adhere to the filter screen surface.
[0045] Furthermore, the device provided by this invention, through its rotating mechanism, can significantly reduce the infection of root mycorrhizal fungi within the filter screen. This device is low in cost, simple to operate, and highly effective.
[0046] In some embodiments, a stainless steel ring 6 is provided at the connection between the frustum-shaped filter screen 1 and the cylindrical filter screen 2.
[0047] For example, the stainless steel ring 6 is welded with copper rods, which ensures a stable connection between the frustum-shaped filter screen 1 and the cylindrical filter screen 2.
[0048] In some examples, handle 3 is mounted on stainless steel ring 6.
[0049] For example, handle 3 is made of stainless steel.
[0050] For example, the handle 3 is made of copper rod welded to the stainless steel ring 6.
[0051] In some embodiments, both the frustum-shaped filter 1 and the cylindrical filter 2 are made of stainless steel, with a pore size of 30-40 μm. This ensures that the pore size only allows mycelia to pass through, while the target root system cannot.
[0052] The following is a detailed description of the use of a stainless steel rotating device for reducing mycorrhizal fungal infection of plant roots provided by this utility model.
[0053] I. Equipment Preparation:
[0054] First, place the stainless steel rotating device of this invention for reducing mycorrhizal fungal infection of plant roots on the operating table and check whether the rotating device is intact.
[0055] II. Sterilization treatment:
[0056] If sterilization of the apparatus is required during the experiment, it can be removed from the rotating experimental platform and placed in a high-temperature, high-pressure sterilizer (equipment temperature: 121℃, pressure: 103kPa, sterilization time: 15-30 minutes). Because the stainless steel woven mesh has excellent high-temperature resistance, its chemical and physical properties will not change under sterilization conditions, effectively killing microorganisms without affecting the structure and performance of the apparatus.
[0057] III. Filling with soil and introducing roots:
[0058] 1. Take an appropriate amount of sterilized soil. The preparation of the sterilized soil must strictly follow the relevant sterilization operation standards to ensure its sterility. Pass the sterilized soil through a funnel, passing it through the flap 7 of the frustum-shaped filter 1, and slowly and evenly fill it into the cylindrical filter 2. During the filling process, gently vibrate the device to ensure the soil is compacted and uniform, avoiding any localized looseness. The amount of soil filled should be adjusted reasonably according to the experimental design and the size of the device. Generally, filling to 5-10 cm from the top of the cylindrical filter 2 is appropriate, leaving space for adding more sterilized soil after the target root system is introduced.
[0059] 2. Carefully place the target root system into the sterilized soil inside the cylindrical filter 2 through the opening 7 of the frustum-shaped filter 1. During this process, tweezers or other tools can be used to avoid damaging the root system, ensuring that the roots spread out naturally and are evenly distributed to simulate the natural growth environment.
[0060] IV. Device Closure and Installation:
[0061] 1. After the soil filling and root planting are completed, use a large-capped three-pronged lantern rivet 4 to pass through the four 4mm round holes 5 on the flap 7 of the frustum-shaped filter 1, and fix the rivet 4 with a rivet gun to ensure that the flap 7 is tightly closed. When fixing, ensure that the flap 7 is secure to prevent it from accidentally opening during device rotation or other operations, which would affect the experimental results.
[0062] 2. After completing the above steps, fix the device in the soil in its original location, ensuring that it is perpendicular to the ground.
[0063] V. Rotation Experiment Procedure:
[0064] 1. Based on the specific requirements of the experiment, set the frequency and angle of the rotation experiment. The rotation frequency can be set to once every 1-2 days, and the rotation angle can be selected between 0-90° to evaluate the effects of physical stimulation and stress on the infection rate of plant root symbiotic mycorrhizal fungi and root growth.
[0065] 2. Throughout the experiment, the condition of the apparatus and the target root system should be closely monitored to ensure that the roots are not damaged during rotation. Regularly check the soil compaction and root growth within the apparatus, noting any entanglement, damage, or other abnormalities in the roots. If any abnormalities are found, the rotation parameters should be adjusted promptly or the apparatus should be maintained.
[0066] VI. Procedures after the experiment:
[0067] After the experiment, remove the device from the soil. Use pliers to open rivet 4 at the flap 7 and carefully remove the soil and root system inside. During removal, the soil and root system must be properly preserved for subsequent analysis. For example, the soil microbial content can be tested, and root growth indicators (length, diameter, number of branches, etc.) and mycorrhizal fungal infection rates can be measured and analyzed.
[0068] VII. Experimental Results:
[0069] like Figure 3 As shown, the fungal infection rate decreased significantly after using the rotating device. (Note: CK is the root infection rate without the device installed, N is the root infection rate with the device installed and not rotated, and X is the root infection rate after rotating once a day for 7 consecutive days.)
[0070] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and are not intended to limit it. Any other modifications or equivalent substitutions made by those skilled in the art to the technical solution of this utility model, as long as they do not depart from the spirit and scope of the technical solution of this utility model, should be covered within the scope of the claims of this utility model.
Claims
1. A stainless steel rotating device for reducing mycorrhizal fungal infestation of plant root systems, characterized by: It comprises: a frustum-shaped filter screen, a cylindrical filter screen, a handle and a rivet; the bottom of the frustum-shaped filter screen is connected to the top of the cylindrical filter screen and is in internal communication, and the top of the frustum-shaped filter screen is provided with an opening for facilitating the introduction of target root systems; the handle is arranged at the connection between the frustum-shaped filter screen and the cylindrical filter screen; a fly is arranged on the side of the frustum-shaped filter screen, and round holes are arranged on the two sides of the fly, through which the rivet passes after the target root system is introduced, so as to fix the fly on the side of the frustum-shaped filter screen.
2. The stainless steel rotating device for reducing the infection of plant root mycorrhizal fungi according to claim 1, wherein a stainless steel ring is arranged at the connection between the frustum-shaped filter screen and the cylindrical filter screen.
3. The stainless steel rotating device for reducing the infection of plant root mycorrhizal fungi according to claim 2, wherein the handle is arranged on the stainless steel ring.
4. The stainless steel rotating device for reducing the infection of plant root mycorrhizal fungi according to claim 1, wherein both the frustum-shaped filter screen and the cylindrical filter screen are made of stainless steel filter screens, and the pore size of the filter screens is 30-40 μm.
5. The stainless steel rotating device for reducing the infection of plant root mycorrhizal fungi according to claim 1, wherein copper rods are used to weld the connection between the frustum-shaped filter screen, the cylindrical filter screen and the handle.
6. The stainless steel rotating device for reducing the infection of plant root mycorrhizal fungi according to claim 1, wherein the handle is made of stainless steel.