Centrifugal tube for exosome extraction

Abstract

The utility model relates to the technical field of centrifugal tubes, and discloses a centrifugal tube for exosome extraction. The centrifugal tube comprises a centrifugal tube assembly, the centrifugal tube assembly comprises a tube body, wherein a concentration tube is arranged in the tube body; the tube plug I is arranged at the top of the tube body; the through hole is formed in the top of the first pipe plug. The pipe plug II is arranged at the top of the through hole; a connecting belt is connected with the tube body, the tube plug I and the tube plug II; according to the centrifugal tube, the concentration tube is placed in the tube body, then the tube plug I is covered to limit and fix the concentration tube, liquid to be separated is dropwise added inwards through the through hole in the top of the tube plug I by using a dropper, then the through hole is plugged by using the tube plug II, and then the tube body is placed in a centrifugal machine to be centrifuged; and the second pipe plug and the first pipe plug form a whole through the connecting belt, and the second pipe plug and the first pipe plug are fixed to the rubber ring and are convenient to disassemble, clean, disinfect and recycle.

Description

TECHNICAL FIELD

[0001] The utility model relates to centrifugal tube technical field, concretely is a kind of centrifugal tube for exosome extraction. BACKGROUND

[0002] Exosome refers to small membrane vesicle (30-150nm) containing complex RNA and protein, nowadays, it refers to disc-shaped vesicle with diameter of 40-100nm. In 1983, exosome was first found in sheep reticulocyte, and in 1987, Johnstone named it "exosome". Various cells can secrete exosome under normal and pathological conditions. It is mainly derived from multivesicular body formed by endocytic lysosome micro-particle, and is released into extracellular matrix after fusion of multivesicular body outer membrane and cell membrane. All cultured cell types can secrete exosome, and exosome naturally exists in body fluid, including blood, saliva, urine, cerebrospinal fluid and milk.

[0003] The existing exosome uses centrifugal tube with different specifications and different types in the extraction process. According to the characteristics of exosome, ultrafiltration centrifugation is preferably carried out, a concentration tube needs to be added in the centrifugal tube, and centrifugation needs to be carried out twice. The cover of the centrifugal tube similar to the thin-walled tube type is inconvenient to open, the operation is complex, and the extraction of exosome is affected, so a new centrifugal tube is set, which is simple in structure, low in cost and high in practicability. UTILITY MODEL CONTENT

[0004] (I) Technical problem solved

[0005] In view of the deficiencies of the prior art, the utility model provides a centrifugal tube for exosome extraction, which has the advantages of convenient use and solves the problem of inconvenient operation of the centrifugal tube during centrifugation.

[0006] (II) Technical scheme

[0007] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a centrifugal tube for exosome extraction, comprising a centrifugal tube assembly; the centrifugal tube assembly comprises: a concentration tube arranged inside a tube body; a plug one is arranged at the top of the tube body; a through hole is arranged at the top of the plug one; a plug two is arranged at the top of the through hole; a connecting belt connects the tube body, the plug one and the plug two.

[0008] In some embodiments, the outer side wall of the tube body is sleeved with a rubber ring, and the connecting belt is connected with the rubber ring.

[0009] In some embodiments, the outer side wall of the plug one is provided with a buckle, and the outer side wall of the rubber ring is provided with a fixing buckle matched with the buckle.

[0010] In some embodiments, an auxiliary assembly is arranged on the centrifugal tube assembly, and the auxiliary assembly comprises: a fixing groove arranged on the outer sidewall of the rubber ring; and a label arranged on the inner part of the rubber ring.

[0011] In some embodiments, a protrusion is arranged on the top of the tube body to match the rubber ring.

[0012] In some embodiments, a barrel is arranged on the bottom of the tube body, and the bottom of the barrel is provided with a base.

[0013] In some embodiments, a sleeve is arranged on the top of the barrel to contain the tube body.

[0014] In some embodiments, the outer sidewall of the barrel is in an inclined structure.

[0015] (III) Beneficial effects

[0016] Compared with the prior art, the centrifugal tube for exosome extraction has the following advantages

[0017] Beneficial effects:

[0018] The centrifugal tube has the following advantages: the concentrated tube is arranged in the inner part of the tube body, and then the tube plug one is used to position and fix the pair of concentrated tubes; the dropper is used to add the liquid to be separated through the through hole in the top of the tube plug one; then the tube plug two is used to block the through hole; the tube body is arranged in the centrifugal machine for centrifugation; the tube plug two and the tube plug one are connected by the connecting belt to form a whole; and the tube plug two and the tube plug one are fixed on the rubber ring, so that the centrifugal tube can be conveniently disassembled, cleaned, disinfected and recycled. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 It is a structural schematic diagram of the centrifugal tube;

[0020] Figure 2 It is a structural schematic diagram of the tube plug one and the tube plug two;

[0021] Figure 3 It is a sectional structural schematic diagram of the tube body;

[0022] Figure 4 It is a structural schematic diagram of the label.

[0023] In the drawings:

[0024] 1, centrifugal tube assembly; 11, tube body; 12, concentrated tube; 13, tube plug one; 14, through hole; 15, tube plug two; 16, rubber ring; 17, connecting belt; 18, buckle; 181, fixing buckle.

[0025] 2, auxiliary assembly; 21, fixing groove; 22, label; 23, protrusion; 24, barrel; 25, base; 26, sleeve. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0027] It should be noted that all directional indications in the embodiments of the present application are only used to explain the relative position relationship, movement condition and the like between components in a certain posture, and if the certain posture changes, the directional indications also change accordingly.

[0028] In the present application, unless otherwise explicitly specified and limited, the terms "connection", "fixation" and the like should be understood in a broad sense, for example, "fixation" can be fixed connection, or detachable connection, or integral; can be mechanical connection, or electrical connection; can be direct connection, or indirect connection through an intermediate medium; can be internal connection of two elements or interaction relationship between two elements, unless otherwise explicitly limited. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0029] In addition, the description such as "first", "second" and the like in the present application is only for the purpose of description, and cannot be understood as indicating or implying the relative importance of the indicated technical features or implicitly indicating the number of the indicated technical features. Therefore, the features limited by "first", "second" can explicitly or implicitly include at least one of the features. In addition, the technical solutions of each embodiment can be combined with each other, but it must be based on the fact that the technical solutions can be realized by those skilled in the art, and when the combination of technical solutions appears contradictory or unachievable, it should be considered that the combination of technical solutions does not exist, and is not within the scope of protection claimed by the present application.

[0030] In the related art, different centrifuge tubes are used in the extraction process of existing exosomes, and different types of centrifuge tubes are used. According to the characteristics of exosomes, ultrafiltration centrifugation is preferably performed, a concentration tube needs to be added in the centrifuge tube, and centrifugation needs to be performed twice. The cover of the thin-walled tube type centrifuge tube is not convenient to open, the operation is complex, and the extraction of exosomes is affected.

[0031] In order to solve the problems in the related art to some extent, the embodiment of the present application provides an exosome extraction centrifuge tube. When it is needed to be used, only a detachable tube plug one 13 and a tube plug two 15 are installed on the tube body 11 to assist the use of the tube body 11 and the concentration tube 12.

[0032] The present application will be described below in conjunction with the drawings and specific embodiments:

[0033] In conjunction with Figures 1-4 , the embodiment of the present application provides an exosome extraction centrifuge tube, which comprises a centrifuge tube assembly 1. The centrifuge tube assembly 1 comprises: a tube body 11, wherein a concentration tube 12 is arranged inside the tube body 11; a tube plug one 13 is arranged on the top of the tube body 11; a through hole 14 is arranged on the top of the tube plug one 13; a tube plug two 15 is arranged on the top of the through hole 14; and a connecting belt 17 connects the tube body 11, the tube plug one 13 and the tube plug two 15.

[0034] In use, first, the concentration tube 12 is placed inside the tube body 11, then the tube plug one 13 is covered to position and fix the concentration tube 12, then a dropper is used to add the liquid to be separated through the through hole 14 on the top of the tube plug one 13, then the tube plug two 15 is used to plug the through hole 14, then the tube body 11 is placed in a centrifuge for centrifugation, and the tube plug two 15 and the tube plug one are integrated through the connecting belt 17.

[0035] Specifically, the tube plug one 13 and the tube plug two 15 are both rubber structures, and the tube body 11 and the concentration tube 12 are transparent structures.

[0036] In some embodiments, the outer side wall of the tube body 11 is sleeved with a rubber ring 16, and the connecting belt 17 is connected with the rubber ring 16. The rubber ring 16 is used to facilitate the fixing of the tube plug two 15 and the tube plug one 13 on the tube body 11 and facilitate the disassembly for sterilization and recycling, thereby reducing the cost and being environmentally friendly.

[0037] In some embodiments, the outer side wall of the tube plug one 13 is provided with a buckle 18, and the outer side wall of the rubber ring 16 is provided with a fixing buckle 181 matched with the buckle 18. The buckle 18 is buckled on the fixing buckle 181, thereby further facilitating the fixing effect between the tube plug one 13 and the tube body 11.

[0038] In some embodiments, an auxiliary assembly 2 is arranged on the centrifuge tube assembly 1, and the auxiliary assembly 2 comprises: a fixing groove 21 arranged on the outer side wall of the rubber ring 16; and a label 22 arranged inside the rubber ring 16. Information is recorded on the label 22, and then the label 22 is placed in the fixing groove 21 of the rubber ring 16 for display. Since the rubber ring 16 itself has elasticity, the label can be well fixed.

[0039] In some embodiments, the top of the tube body 11 is provided with a protrusion 23 matching the rubber ring 16. The protrusion 23 is to avoid the rubber ring 16 from being taken off from the top of the tube body 11.

[0040] In some embodiments, the bottom of the tube body 11 is provided with a cylinder 24, and the bottom of the cylinder 24 is provided with a base 25. The combination of the cylinder 24 and the base 25 is to facilitate the standing of the tube body 11, and when a small amount of centrifugal operation is performed, the test tube rack can be abandoned, and the carrying is convenient.

[0041] In some embodiments, the top of the cylinder 24 is provided with a sleeve 26, and the sleeve 26 contains the tube body 11. The sleeve 26 is sleeved from the top of the tube body 11 and connected with the cylinder 24, which facilitates the sealing of the tube body 11.

[0042] In some embodiments, the outer side wall of the cylinder 24 is inclined. The sleeve 26 and the cylinder 24 are clamped with each other.

[0043] In the description of the present specification, the description of the terms "one embodiment", "some embodiments", "example", "specific example", "some examples" and the like means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are contained in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in the present specification.

[0044] In addition, the technical solutions of each embodiment can be combined with each other, but it must be based on the realization of the ordinary skilled in the art, when the combination of technical solutions appears contradictory or cannot be realized, it should be considered that the combination of technical solutions does not exist, also not within the scope of protection required by the present application.

[0045] Although the embodiments of the present application have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to the embodiments without departing from the principles and spirits of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A centrifuge tube for exosome extraction, characterized by: The centrifugal tube assembly (1) comprises: a tube body (11) with a condenser tube (12) inside; a first tube plug (13) on the top of the tube body (11); a through hole (14) on the top of the first tube plug (13); a second tube plug (15) on the top of the through hole (14); a connecting band (17) connecting the tube body (11), the first tube plug (13) and the second tube plug (15).

2. The centrifuge tube for exosome extraction according to claim 1, characterized in that: The outer wall of the tube body (11) is sleeved with a rubber ring (16), and the connecting band (17) is connected with the rubber ring (16).

3. The centrifuge tube for exosome extraction according to claim 2, characterized in that: The outer wall of the first tube plug (13) is provided with a buckle (18), and the outer wall of the rubber ring (16) is provided with a fixing buckle (181) matched with the buckle (18).

4. The centrifuge tube for exosome extraction according to claim 2, characterized in that: An auxiliary assembly (2) is arranged on the centrifugal tube assembly (1), and the auxiliary assembly (2) comprises: a fixing groove (21) on the outer wall of the rubber ring (16); a label (22) arranged inside the rubber ring (16).

5. The centrifuge tube for exosome extraction according to claim 2, characterized in that: The top of the tube body (11) is provided with a protrusion (23) matched with the rubber ring (16).

6. The centrifuge tube for exosome extraction according to claim 2, characterized in that: The bottom of the tube body (11) is provided with a barrel (24), and the bottom of the barrel (24) is provided with a base (25).

7. The centrifuge tube for exosome extraction according to claim 6, characterized in that: The top of the barrel (24) is provided with a sleeve (26), and the sleeve (26) contains the tube body (11).

8. The centrifuge tube for exosome extraction according to claim 7, characterized in that: The outer wall of the barrel (24) is inclined.

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