Suspension cell sampling device

Abstract

The utility model relates to the technical field of cell sampling, and discloses a suspension cell sampling device which comprises a sealing assembly and a sampling assembly, the sealing assembly comprises a sealing plug, a stable sleeve hole is formed in the sealing plug, a sealing cover is movably connected to the side portion of the sealing plug, the sampling assembly comprises a limiting rod movably connected in the stable sleeve hole in a sleeved mode, and the limiting rod is connected with the stable sleeve hole in a sleeved mode. A connecting rod and a mounting cylinder are fixed on a limiting rod, a sealing plug is inserted into a connector of a loading container, and a regulating gear is shifted up and down, so that the limiting rod drives the whole sampling assembly to move up and down until the bottom of a buoyancy plate is attached to liquid, and a sampling needle head is positioned at the top of the liquid, and cells floating on the liquid are collected; therefore, the problem that a traditional sampling mode is unstable is effectively avoided, the overall height of the sampling assembly can be fixed by rotating the positioning screw, the sampling assembly can stably collect the suspension cells at the height position, and the practicability of the sampling assembly is effectively improved.

Description

Technical Field

[0001] This utility model relates to the field of cell sampling technology, specifically a suspended cell sampling device. Background Technology

[0002] Suspension cells are cells that float freely in liquid culture media and are not attached to solid surfaces. These cells usually exist in a uniform distribution in the culture medium and are commonly found in blood cells, certain tumor cell lines, and immune cells. They can grow and multiply independently in the culture medium and are widely used in clinical diagnosis, basic research and development, and drug development. They are mainly stored in test tubes and culture flasks. When sampling suspension cells, a special sampling device is required to collect the suspension cells from the container.

[0003] Currently, most existing suspended cell sampling devices use a dropper to extract suspended cells from the container. This method is convenient and quick, but due to the instability of manual operation, the liquid at the bottom of the dropper is easily disturbed, causing shaking and damage to the suspended cells, resulting in difficulties in sampling suspended cells. Utility Model Content

[0004] (a) Technical problems to be solved

[0005] To address the shortcomings of existing technologies, this invention provides a suspended cell sampling device that has the advantages of being able to stably sample suspended cells in a loading container, thus solving the problem of damage to suspended cells caused by liquid sloshing when using a dropper for manual sampling.

[0006] (II) Technical Solution

[0007] To achieve the above objectives, this utility model provides the following technical solution: a suspended cell sampling device, comprising a sealing component and a sampling component. The sealing component includes a sealing plug with a stabilizing sleeve hole inside. A sealing cap is movably connected to the side of the sealing plug. The bottom end of the sealing plug is inserted into the interface of the loading container. The sealing cap is made of rubber and can be fitted onto the side of the interface, achieving a double sealing effect to prevent liquid leakage and external dust and impurities from entering the bottle.

[0008] The sampling assembly includes a limiting rod that is movably fitted into a stabilizing sleeve hole. A connecting rod and a mounting cylinder are fixed on the limiting rod. The limiting rod, connecting rod, and mounting cylinder have interconnected mounting holes. A sampling needle is movably fitted into the mounting holes. The diameters of the limiting rod, connecting rod, and mounting cylinder match the diameter of the stabilizing sleeve hole, improving the stability of the rod's vertical movement within the sleeve hole. By moving vertically, the assembly can sample suspended cells at different heights within the bottle.

[0009] As a further improvement to the above solution, a rubber layer is fixed to the side of the sealing plug, and the sealing cap is movably sleeved on the side of the rubber layer.

[0010] Through the above technical solution, a rubber layer is fixed on the bottom half of the sealing plug, and a round hole is opened on the sealing cap. The diameter of the round hole matches the diameter of the side of the sealing plug. The sealing cap is made of a flexible rubber material and is fitted onto the side of the loading container interface with different diameters, thereby improving the sealing performance of the sealing cap fitted onto the side of the sealing plug.

[0011] As a further improvement to the above solution, the side of the sealing plug is provided with an installation groove, which passes through the stabilizing sleeve hole, and an adjusting gear is rotatably connected in the installation groove. At the same time, the side of the limiting rod is provided with a limiting groove, and a tooth profile is provided in the limiting groove, and the adjusting gear meshes with the tooth profile.

[0012] Through the above technical solution, the width of the regulating gear matches the width of the limiting groove, preventing the limiting rod from rotating and improving the stability of the limiting rod's lifting and lowering. When the regulating gear is moved up and down, the limiting rod drives the entire sampling component to move up and down under the meshing force between the tooth pattern and the tooth profile.

[0013] As a further improvement to the above solution, the sealing plug has a positioning hole on its side, which penetrates the stabilizing sleeve hole and is threadedly connected to a positioning screw, one end of which is attached to the side of the limiting rod.

[0014] Through the above technical solution, by rotating the positioning screw in the forward or reverse direction, the front end of the positioning screw is made to be close to or away from the side of the limiting rod by the force of the connection between the screw side thread and the internal thread of the positioning hole. When the front end of the screw is close to the side of the limiting rod, the height position of the limiting rod is fixed. When the front end of the screw is away from the side of the limiting rod, the limiting rod can move up and down to adjust the height.

[0015] As a further improvement to the above solution, a rubber ring is movably sleeved at one end of the connecting rod, and a buoyancy plate is fixed on the side of the rubber ring.

[0016] Through the above technical solution, the inner diameter of the rubber ring matches the diameter of the side of the connecting rod. The position of the buoyancy plate is fixed by the frictional resistance between the inner side of the rubber ring and the side of the connecting rod. The buoyancy plate can generate buoyancy. With the buoyancy plate installed at the bottom of the connecting rod and the bottom of the buoyancy plate flush with the bottom of the sampling needle, the sampling needle can sample the cells that are uniformly dispersed in the liquid culture medium.

[0017] As a further improvement to the above solution, a sampling airbag is fixed on the mounting cylinder, and one end of the sampling needle is inserted into the sampling airbag.

[0018] With the above technical solution, a circular interface is provided on the installation cylinder, and the interface of the sampling airbag is installed in the circular interface, so that the tip of the sampling needle can be inserted into the interface of the sampling airbag. At the same time, the entire sampling airbag is made of soft rubber material, and the interface can be tightly attached to the side of the sampling needle to achieve a sealing effect and prevent air leakage at the connection. Thus, when the sampling airbag is squeezed, the sampling needle can effectively sample suspended cells.

[0019] As a further improvement to the above solution, the side of the mounting cylinder is threaded with a retaining screw, one end of which is attached to the sampling needle.

[0020] With the above technical solution, the side of the mounting cylinder is provided with a threaded hole that matches the diameter of the screw. The retaining screw is threaded into the threaded hole. By rotating in the forward or reverse direction, the front end of the retaining screw can be pressed against or disengaged from the corresponding position on the side of the sampling needle. When the front end of the retaining screw is pressed against the side of the sampling needle, one end of the sampling needle is fixed in the mounting cylinder. When the front end of the retaining screw is disengaged from the side of the sampling needle, the sampling needle can be separated from the mounting cylinder and the sampling airbag.

[0021] Compared with the prior art, this utility model provides a suspended cell sampling device, which has the following features:

[0022] Beneficial effects:

[0023] 1. This suspended cell sampling device is inserted into the interface of the loading container through a sealing plug. By moving the control gear up and down, the limiting rod drives the entire sampling component to move up and down until the bottom of the buoyancy plate is in contact with the liquid, so that the sampling needle is at the top of the liquid, thereby collecting cells floating on the liquid. This effectively avoids the instability problem of traditional sampling methods. Furthermore, the height of the entire sampling component can be fixed by rotating the positioning screw, so that the sampling component can be kept at this height to stably collect suspended cells, effectively improving its practicality.

[0024] 2. This suspended cell sampling device uses a sampling needle tube that is movably connected to a limiting rod and a connecting rod. The top end of the needle tube is connected to the sampling airbag via an insertion method and secured by a fixing screw. After sampling, the front end of the needle can be disengaged from the side of the sampling needle by rotating the fixing screw in the opposite direction. This allows the sampling needle to be pulled out and separated from the sampling airbag, facilitating subsequent experiments. Furthermore, a new sampling needle can be replaced to complete continuous sampling, further improving the flexibility and practicality of the device. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of the overall external top view of the device of this utility model;

[0026] Figure 2 This is a schematic diagram of the overall external bottom structure of the device of this utility model;

[0027] Figure 3 This is a schematic diagram of the overall disassembled structure of the sampling component of this utility model;

[0028] Figure 4 This is a schematic diagram of the disassembled structure of the sealing component of this utility model.

[0029] The attached diagram lists the components represented by each number as follows:

[0030] 1. Sealing assembly; 101. Sealing plug; 102. Retaining sleeve hole; 103. Rubber layer; 104. Mounting groove; 105. Adjusting gear; 106. Positioning hole; 107. Positioning screw; 108. Sealing cap;

[0031] 2. Sampling assembly; 201. Limiting rod; 202. Limiting groove; 203. Tooth profile; 204. Connecting rod; 205. Mounting hole; 206. Rubber ring; 207. Buoyancy plate; 208. Mounting cylinder; 209. Sampling airbag; 210. Fixing screw; 211. Sampling needle. Detailed Implementation

[0032] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0033] Example 1

[0034] Please see Figure 1-4 As shown, the suspended cell sampling device proposed in this embodiment includes a sealing component 1 and a sampling component 2. The sealing component 1 includes a sealing plug 101, which has a stabilizing sleeve hole 102. A sealing cap 108 is movably connected to the side of the sealing plug 101. The bottom end of the sealing plug 101 is inserted into the interface of the loading container. The sealing cap 108 is made of rubber and can be fitted onto the side of the interface to achieve a double sealing effect, preventing liquid leakage and external dust and impurities from entering the bottle.

[0035] The sampling assembly 2 includes a limiting rod 201 that is movably fitted into the stabilizing sleeve hole 102. A connecting rod 204 and a mounting cylinder 208 are fixed on the limiting rod 201. The limiting rod 201, the connecting rod 204 and the mounting cylinder 208 are provided with interconnecting mounting holes 205. A sampling needle 211 is movably fitted into the mounting hole 205. The diameters of the limiting rod 201, the connecting rod 204 and the mounting cylinder 208 match the diameter of the stabilizing sleeve hole 102, which improves the stability of the rod's vertical movement within the sleeve hole. By moving vertically, the assembly can sample suspended cells at different heights within the bottle.

[0036] The working principle of the suspended cell sampling device proposed in this embodiment is as follows: During use, the sampling needle 211 is fitted into the mounting hole 205 formed by the limiting rod 201, connecting rod 204, and mounting cylinder 208, and one end of the sampling needle 211 is inserted into the interface of the sampling airbag 209. By rotating the fixing screw 210, the front end of the fixing screw 210 is pressed tightly against the side of the corresponding position of the sampling needle 211, thus fixing the sampling needle 211. The bottom end of the sealing plug 101 is inserted into the loading container interface, causing the rubber layer 103 to press tightly against the inner side of the interface for initial sealing. Then, the sealing cap 108 is movably fitted onto the side of the rubber layer 103 (under the elastic force of the rubber layer 103, it can better fit into the hole where the sealing cap 108 is fitted, increasing the sealing performance at the interface). Simultaneously, the sealing cap 108 is movably fitted onto the outside of the loading container interface, achieving secondary sealing and preventing liquid leakage or dust and debris from falling into the interior. After assembly, by moving the adjusting gear 105 up and down, the gear meshes with the tooth profile 203, causing the sampling assembly 2 to move up and down until the bottom of the buoyancy plate 207 floats above the liquid in the container. At this point, the bottom of the sampling needle 211 is on the liquid surface. After adjusting to the appropriate angle, rotate the positioning screw 107 so that the front end of the screw is in close contact with the side of the limiting rod 201 to fix the height. Then, by squeezing the sampling airbag 209... The sampling needle 211 extracts suspended cells (the extracted cells are inside the sampling needle 211). After extraction, the sealing cap 108 is removed from the bottle body interface, and the sealing plug 101 and sampling component 2 are removed simultaneously. By rotating the reverse retention screw 210, the front end of the retention screw 210 is removed from the side of the sampling needle 211, and then the sampling needle 211 is removed. After installing a new sampling needle 211, the above steps are repeated for other sampling operations.

[0037] Furthermore, a rubber layer 103 is fixed to the side of the sealing plug 101, and the sealing cap 108 is movably sleeved on the side of the rubber layer 103.

[0038] More specifically, a rubber layer 103 is fixed on the bottom half of the sealing plug 101, while a circular hole is opened on the sealing cap 108. The diameter of the circular hole matches the diameter of the side of the sealing plug 101. The sealing cap 108 is made of a flexible rubber material and is fitted onto the side of the loading container interface with different diameters, thereby improving the sealing performance of the sealing cap 108 fitted onto the side of the sealing plug 101.

[0039] Furthermore, the side of the sealing plug 101 is provided with an installation groove 104, which passes through the stabilizing sleeve hole 102, and an adjusting gear 105 is rotatably connected in the installation groove 104. At the same time, the side of the limiting rod 201 is provided with a limiting groove 202, and a tooth profile 203 is provided in the limiting groove 202. The adjusting gear 105 meshes with the tooth profile 203.

[0040] More specifically, the width of the regulating gear 105 matches the width of the limiting groove 202, preventing the limiting rod 201 from rotating and improving the stability of the lifting and lowering of the limiting rod 201. When the regulating gear 105 is moved up and down, the limiting rod 201 drives the sampling component 2 to move up and down as a whole through the meshing force between the tooth pattern and the tooth profile 203.

[0041] Furthermore, the sealing plug 101 has a positioning hole 106 on its side, which penetrates the stabilizing sleeve hole 102 and is threadedly connected to a positioning screw 107. One end of the positioning screw 107 is attached to the side of the limiting rod 201.

[0042] More specifically, by rotating the positioning screw 107 in the forward or reverse direction, the front end of the positioning screw 107 is made to be close to or detach from the side of the limiting rod 201 by the force of the connection between the screw side thread and the internal thread of the positioning hole 106. When the front end of the screw is close to the side of the limiting rod 201, the height position of the limiting rod 201 is fixed. When the front end of the screw is detached from the side of the limiting rod 201, the limiting rod 201 can move up and down to adjust the height.

[0043] Furthermore, a rubber ring 206 is movably sleeved on one end of the connecting rod 204, and a buoyancy plate 207 is fixed on the side of the rubber ring 206.

[0044] More specifically, the inner diameter of the rubber ring 206 matches the diameter of the side of the connecting rod 204. The frictional resistance between the inner side of the rubber ring 206 and the side of the connecting rod 204 fixes the position of the buoyancy plate 207. The buoyancy plate 207 can generate buoyancy. With the buoyancy plate 207 installed at the bottom of the connecting rod 204 and the bottom of the buoyancy plate 207 flush with the bottom of the sampling needle 211, the sampling needle 211 can sample the cells that are uniformly dispersed in the liquid culture medium.

[0045] Furthermore, a sampling airbag 209 is fixed on the mounting cylinder 208, and one end of the sampling needle 211 is inserted into the sampling airbag 209.

[0046] More specifically, the mounting cylinder 208 has a circular interface, and the interface of the sampling airbag 209 is installed in the circular interface, so that the tip of the sampling needle 211 can be inserted into the interface of the sampling airbag 209. At the same time, the sampling airbag 209 is made of soft rubber material, and the interface can fit tightly against the side of the sampling needle 211 to achieve a sealing effect and prevent air leakage at the connection. Thus, when the sampling airbag 209 is squeezed, the sampling needle 211 can effectively sample the suspended cells.

[0047] Furthermore, the side of the mounting cylinder 208 is threaded with a retaining screw 210, one end of which is attached to the sampling needle 211.

[0048] More specifically, the side of the mounting cylinder 208 is provided with a threaded hole that matches the diameter of the screw. The retaining screw 210 is threaded into the threaded hole. By rotating in the forward or reverse direction, the front end of the retaining screw 210 can be pressed against or disengaged from the corresponding position on the side of the sampling needle 211. When the front end of the retaining screw 210 is pressed against the side of the sampling needle 211, one end of the sampling needle 211 is fixed in the mounting cylinder 208. When the front end of the retaining screw 210 is disengaged from the side of the sampling needle 211, the sampling needle 211 can be separated from the mounting cylinder 208 and the sampling airbag 209.

[0049] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A suspended cell sampling device comprising a seal assembly, a sampling assembly, characterized in that: The sealing assembly comprises a sealing plug, a stable sleeve hole is formed in the sealing plug, and a sealing cover is movably connected to the side of the sealing plug; The sampling assembly comprises a limiting rod movably sleeved in the stable sleeve hole, a connecting rod and a mounting cylinder are fixed to the limiting rod, and an installation hole penetrating through the limiting rod, the connecting rod and the mounting cylinder is formed in the limiting rod, the connecting rod and the mounting cylinder, and a sampling needle is movably sleeved in the installation hole.

2. A suspended cell sampling device according to claim 1, wherein: The side of the sealing plug is fixed with a rubber layer, and the sealing cover is movably sleeved on the side of the rubber layer.

3. A suspended cell sampling device according to claim 2, wherein: The side of the sealing plug is provided with an installation groove, the installation groove penetrates through the stable sleeve hole, a control gear is rotatably connected in the installation groove, the side of the limiting rod is provided with a limiting groove, a tooth profile is arranged in the limiting groove, and the control gear is engaged with the tooth profile.

4. A suspended cell sampling device according to claim 3, wherein: The side of the sealing plug is provided with a positioning hole, the positioning hole penetrates through the stable sleeve hole, and a positioning screw is threadedly connected to the stable sleeve hole, and one end of the positioning screw is attached to the side of the limiting rod.

5. The suspended cell sampling device of claim 1, wherein: One end of the connecting rod is movably sleeved with a rubber ring, and a buoyancy plate is fixed to the side of the rubber ring.

6. The suspended cell sampling device of claim 1, wherein: The sampling gas bag is fixed to the mounting cylinder, and one end of the sampling needle is inserted into the sampling gas bag.

7. A suspended cell sampling device according to claim 6, wherein: The side of the mounting cylinder is threadedly connected with a retaining screw knob, and one end of the retaining screw knob is attached to the sampling needle.

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