Autoimmune liver disease antibody detection chip

Abstract

The utility model discloses an autoimmune liver disease antibody detection chip, which belongs to the technical field of biochips and diagnostic reagents and comprises a reaction membrane, an upper shell and a lower shell, the upper shell and the lower shell can be buckled together, a water absorption layer is arranged between the upper shell and the lower shell, a blood filtering component is movably arranged above the upper shell, and a water outlet is formed in the lower shell. The blood filtering assembly comprises a blood filtering cup and a blood filtering film, and the blood filtering film is fixedly connected with the blood filtering cup. According to the autoimmune liver disease antibody detection chip disclosed by the utility model, the blood filtering film can be flexibly mounted and dismounted, and the waste of the blood filtering film is avoided.

Description

Technical Field

[0001] This utility model relates to the field of biochip and diagnostic reagent technology, and in particular to an autoimmune liver disease antibody detection chip. Background Technology

[0002] Autoimmune liver diseases (AILD) are a group of liver diseases caused by abnormalities in the autoimmune system, primarily manifesting as liver damage and abnormal liver function. AILD has an insidious onset, and its clinical manifestations are similar to other common liver diseases, such as jaundice, fatigue, abdominal pain, and itching. Some patients may also have extrahepatic autoimmune diseases such as rheumatism, gastrointestinal diseases, lung diseases, and skin diseases. Therefore, the development of specific antibody detection chips for AILD is crucial.

[0003] Traditional detection chips require the removal of blood cells and the collection of serum or plasma for testing. This necessitates centrifugation of whole blood, a cumbersome process prone to errors. Chinese Patent CN 118207290 B discloses a dry chemical test strip and detection method for multiple combined detection, comprising a substrate, a reaction membrane, a filter membrane, a blood filtration membrane, and a diffusion membrane. The substrate has one or more separate reaction membranes, the reaction membranes have one or more separate filter membranes, the filter membranes have a single blood filtration membrane, and the blood filtration membrane has a single diffusion membrane. Each reaction membrane is independent, and each reaction membrane is loaded with the corresponding enzyme reactant and chromogenic agent. While the blood filtration membrane can filter blood cells, it is assembled within the chip. When the sample is plasma or serum, the blood filtration membrane is ineffective, resulting in waste and increased testing costs. Utility Model Content

[0004] The technical problem to be solved by this invention is to design a novel autoimmune liver disease antibody detection chip, whose blood filtration membrane can be freely assembled without wasting the blood filtration membrane.

[0005] To solve the above-mentioned technical problems, the present invention adopts the following technical solution: an autoimmune liver disease antibody detection chip, including a reaction membrane, an upper shell and a lower shell that can be snapped together, an absorbent layer placed between the upper shell and the lower shell, and a blood filtration assembly movably disposed above the upper shell, the blood filtration assembly including a blood filtration cup and a blood filtration membrane, the blood filtration membrane being fixedly connected to the blood filtration cup.

[0006] Furthermore, the blood filtration membrane is fixed to the bottom or inside of the blood filtration cup.

[0007] Furthermore, the blood filtration membrane is V-shaped in the middle and fixed to the blood filtration cup at both ends.

[0008] Furthermore, a sample application window is provided on the upper part of the upper shell, and the blood filtration assembly is movably positioned above the observation window.

[0009] Furthermore, the reaction membrane is disposed at the bottom of the sample application window.

[0010] Furthermore, the detection chip also includes a movable block that engages in the sample application window, and the reaction membrane is fixed between the movable block and the observation window.

[0011] Furthermore, the inner side of the sample application window is provided with several grooves, the lower part of the grooves is provided with support grooves, and the corresponding position of the movable block is provided with protrusions.

[0012] Furthermore, the reaction membrane is coated with one or more of the following antigens: AMA-M2 antigen, Sp100 antigen, LKM-1 antigen, LC-1 antigen, SLA / LP antigen, Ro-52 antigen, and CENP B antigen.

[0013] Beneficial effects: By setting up a movable blood filtration component, when the sample to be tested is whole blood, the blood filtration component can be placed on the upper part of the sample application window, and the sample is dripped onto the blood filtration membrane. After filtering out red blood cells, it drips onto the reaction membrane. When the sample to be tested is plasma or serum, the blood filtration component does not need to be placed, and the sample is directly dripped onto the reaction membrane through the sample application window. The operation is more flexible, avoids waste of the blood filtration membrane, and reduces the cost of use.

[0014] Meanwhile, this application uses a movable block to hold the reaction membrane inside the sample application window. During production, the upper and lower shells and the absorbent layer can be assembled outside the clean area, while only the movable block and the reaction membrane need to be assembled inside the clean area, saving production time and increasing production efficiency. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of the detection chip of this utility model.

[0016] Figure 2 This is a schematic diagram of the blood filtration component of this utility model.

[0017] Figure 3 This is a cross-sectional schematic diagram of the blood filtration component of this utility model.

[0018] Figure 4 This is a schematic diagram of the blood filtration component after installation.

[0019] Figure 5 This is a schematic diagram of the sample window structure of this utility model.

[0020] Figure 6 This is a schematic diagram of the structure of the movable block of this utility model.

[0021] Wherein 1-blood filtration cup, 2-blood filtration membrane, 3-live block, 311-protrusion, 4-reaction membrane, 5-upper shell, 51-sample application window, 511-groove, 512-support groove, 6-absorbent layer, 7-lower shell. Detailed Implementation

[0022] To enhance understanding of this utility model, it will be described in detail below with reference to the embodiments and accompanying drawings. These embodiments are only used to explain this utility model and do not limit the scope of protection of this utility model.

[0023] like Figure 1 The image shows an autoimmune liver disease antibody detection chip, comprising an upper shell 5 and a lower shell 7 that can be snapped together, with an absorbent layer 6 placed between the upper shell 5 and the lower shell 7. A sample application window 51 is provided in the upper part of the upper shell 5, penetrating the upper shell, and a reaction membrane 4 is placed at the bottom or inside of the sample application window 51.

[0024] A blood filtration assembly is movably disposed on the upper part of the upper shell 5. The blood filtration assembly includes a blood filtration cup 1 and a blood filtration membrane 2, and the blood filtration membrane 2 is fixedly connected to the blood filtration cup 1.

[0025] Preferred, such as Figure 2 and 3 As shown, the middle of the blood filtration membrane 2 is V-shaped, and both ends are fixed to the bottom of the blood filtration cup 1. This can be achieved by adhesive bonding. Alternatively, both ends of the blood filtration membrane 2 can be inserted into the inside of the blood filtration cup 1 for fixation; the fixation method is not limited here.

[0026] The V-shaped filtration membrane allows the sample to concentrate at the bottom of the membrane, thus enabling more accurate dripping into the reaction membrane below.

[0027] like Figure 4 The diagram shows the structure of the detection chip after the blood filtration assembly is installed. The blood filtration cup 1 serves to fix the blood filtration membrane 2 and place the blood filtration assembly on the observation window 51. Therefore, it is a hollow structure, and the sample to be tested can be directly added to the blood filtration membrane 2.

[0028] like Figure 5 and Figure 6 As shown, the detection chip also includes a movable block 3. Several grooves 511 are provided on the inner side of the sample application window 51, and a protrusion 311 is provided at the corresponding position of the movable block 3. The movable block 3 can be engaged in the grooves 511 of the sample application window 51 by the protrusion 311.

[0029] A support groove 512 is provided at the lower part of the groove 511. The edge of the reaction membrane 4 can be inserted into the support groove 512. When the movable block 3 engages with the sample application window 51, the reaction membrane 4 will be fixed between the movable block 3 and the observation window 51. This design can realize the rapid installation of the reaction membrane during the production process.

[0030] Preferably, the reaction membrane 4 is an NC membrane, which is coated with one or more of the following specific antigens of autoimmune liver disease: AMA-M2 antigen, Sp100 antigen, LKM-1 antigen, LC-1 antigen, SLA / LP antigen, Ro-52 antigen, and CENP B antigen.

[0031] The embodiments disclosed herein are preferred embodiments, but are not limited thereto. Those skilled in the art can readily grasp the spirit of this utility model based on the above embodiments and make different extensions and variations. However, as long as they do not depart from the spirit of this utility model, they are all within the protection scope of this utility model.

Claims

1. An autoimmune liver disease antibody detection chip, comprising a reaction film (4), an upper shell (5) and a lower shell (7) which can be buckled together, and a water absorption layer (6) placed between the upper shell (5) and the lower shell (7), characterized in that, The upper shell (5) is movably provided with a blood filtering assembly, which comprises a blood filtering cup (1) and a blood filtering membrane (2) fixedly connected with the blood filtering cup (1).

2. The autoimmune liver disease antibody detection chip according to claim 1, characterized by, The blood filtering membrane (2) is fixed to the bottom or inside of the blood filtering cup (1).

3. The autoimmune liver disease antibody detection chip according to claim 2, characterized by, The blood filtering membrane (2) is V-shaped in the middle and fixed to the blood filtering cup (1) at both ends.

4. The autoimmune liver disease antibody detection chip according to claim 1, characterized by, The upper shell (5) is provided with a sample adding window (51) at the upper portion, and the blood filtering assembly is movably placed above the sample adding window (51).

5. The autoimmune liver disease antibody detection chip according to claim 4, characterized by, The reaction membrane (4) is arranged at the bottom of the sample adding window (51).

6. The autoimmune liver disease antibody detection chip according to claim 4, wherein, The detection chip further comprises a movable block (3) clamped in the sample adding window (51), and the reaction membrane (4) is fixed between the movable block (3) and the sample adding window (51).

7. The autoimmune liver disease antibody detection chip according to claim 6, characterized in that, The inner side of the sample adding window (51) is provided with a plurality of grooves (511), the lower portion of each groove (511) is provided with a supporting groove (512), and the movable block (3) is provided with a protrusion (311) at a corresponding position.

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