Teaching kit for demonstrating transcription and translation results in stages
By designing a teaching kit that demonstrates transcription and translation results in stages, the problem of the inability to observe the transcription process in existing technologies has been solved, thereby improving student participation and the accuracy of experimental results.
Patent Information
- Application Number
- CN202423281999.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-30
- Publication Date
- 2026-01-09
- Estimated Expiration
- 2034-12-30
AI Technical Summary
Existing teaching kits cannot visually demonstrate the transcription and translation process, resulting in insufficient student participation and experience, and they also cannot observe the transcription process.
Design a teaching kit for demonstrating transcription and translation results in stages. The kit includes a kit body, a manipulation module, a transcription reaction module, a translation reaction module, a transcription heating module, a transcription observation module, and a translation observation module. The kit enables the movement and temperature control of the reaction tube through guide slides and a heat insulation unit, and provides a transparent observation window and a specific light source to observe the reaction process.
This approach enables phased observation of the transcription and translation processes, enhancing student engagement and experience, and ensuring the accuracy and reliability of experimental results.
Smart Images
Figure CN223784793U_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of reagent kit technology, and in particular to a teaching reagent kit for demonstrating transcription and translation results in stages. Background Technology
[0002] Current technologies have successfully enabled the expression and production of large quantities of proteins. However, due to various factors, some biologically significant proteins, such as cytotoxic proteins and poorly soluble proteins, remain difficult to express efficiently. With the continuous advancement of science and technology, cell-free protein expression technology, due to its unique open system characteristics, can precisely control reaction conditions, regulate reactant composition, and achieve direct monitoring of the reaction process, thus becoming a powerful protein expression method.
[0003] Cell-free protein synthesis (CFPS) systems, as an in vitro gene expression technology, utilize exogenous DNA or mRNA as templates and synthesize proteins in a cell-extracted environment by artificially supplementing essential raw materials and energy substances. This system can overcome the inherent limitations of cells, enabling rapid and convenient expression of a variety of proteins. Specifically, the CFPS system uses DNA as a template, and under the combined action of components such as RNA polymerase and transcription factors, a transcription process occurs to produce the corresponding mRNA. Subsequently, using mRNA as a template, and with the help of ribosomes, amino acid substrates, tRNA, and energy substances within the system, protein translation and synthesis are completed. Currently, core concepts in synthetic biology, such as protein expression, mainly exist in textbooks in theoretical form. Students often find these concepts abstract and vague, leading to a lack of interest and difficulty in understanding them, thus affecting their learning efficiency.
[0004] The central dogma plays a crucial role in protein synthesis and regulation. Proposed by Francis Crick in 1958, it is one of the fundamental theories of modern biology. It describes the flow of genetic information within an organism: from DNA to RNA, and then from RNA to protein, completing the transcription and translation processes. However, current learning of the central dogma remains at the conceptual level, neglecting the practical application of knowledge and the cultivation of students' application and innovation abilities. To enable students to more intuitively understand the transcription and translation processes, a teaching kit that demonstrates the results of transcription and translation in stages is needed.
[0005] While Jessica C. Stark offers a simple, intuitive, and relatively inexpensive educational kit in the current technology, it is limited to demonstrating the final result from DNA to protein and cannot observe the transcription process. Furthermore, since the kit is designed for use by 30 students, this may reduce the individual student's participation and experience during the experiment. Therefore, there is an urgent need for an educational kit that provides a visual understanding of the transcription and translation processes. Utility Model Content
[0006] The main objective of this invention is to provide a teaching kit for demonstrating transcription and translation results in stages, thereby overcoming the shortcomings of existing technologies. To achieve the aforementioned objective, the technical solution adopted by this invention includes:
[0007] This invention provides a teaching kit for demonstrating transcription and translation results in stages. The kit includes a kit body, a control module, a transcription reaction module, a translation reaction module, and intervalically arranged transcription heating module, transcription observation module, translation heating module, and translation observation module. These modules are encapsulated within the kit body. The control module is connected to the transcription heating module, transcription observation module, translation heating module, and translation observation module to control the transcription and translation processes.
[0008] The transcription reaction module can be transferred between the transcription heating module and the transcription observation module, and the translation reaction module can be transferred between the translation heating module and the translation observation module. The transcription heating module is used to heat the transcription reaction module to provide the transcription reaction temperature for the transcription reaction to occur, the translation heating module is used to heat the translation reaction module to provide the translation reaction temperature for the translation reaction to occur, the transcription observation module is used to provide the observation conditions for observing the transcription reaction, and the translation observation module is used to provide the observation conditions for observing the translation reaction.
[0009] In some more specific implementations, the transcription heating module includes a transcription heating cavity and a first electrothermal mechanism. The transcription reaction module is disposed within the transcription heating cavity and has a transcription reaction cavity for performing the transcription reaction. The first electrothermal mechanism is connected to the transcription heating cavity and is used to maintain the temperature within the transcription heating cavity at a transcription reaction temperature that allows the transcription reaction to occur.
[0010] Preferably, the transcription reaction module is a transcription reaction tube, which is hollow inside and has a reagent inlet on its wall, and a transcription reaction chamber inside the transcription reaction tube.
[0011] Preferably, the transcription heating chamber is provided with a first guide slide, through which the transcription reaction module can enter and exit the transcription heating chamber.
[0012] Preferably, the first electrothermal mechanism is disposed at the bottom of the transcription heating chamber.
[0013] In some more specific implementations, the transcription observation module includes a transcription observation chamber and a transcription observation light source. The transcription reaction module is disposed inside the transcription observation chamber, and the transcription observation light source is connected to the transcription observation chamber. The transcription observation light source is used to provide illumination conditions for observing the transcription reaction.
[0014] Preferably, a second guide slide is provided inside the transcription observation chamber, through which the transcription reaction module can enter and exit the transcription observation chamber.
[0015] Preferably, the transcription observation light source is located at the bottom of the transcription observation chamber.
[0016] In some more specific implementations, the top of the kit is provided with a transcription observation window, which is a transparent viewing window located above the transcription observation chamber, for observing the transcription process within the transcription reaction module.
[0017] Preferably, the transcription observation module further includes a transcription filter unit, which is disposed at the top of the transcription observation chamber and covers the transcription observation window.
[0018] In some more specific implementations, the translation heating module includes a translation heating cavity and a second electrothermal mechanism. The second electrothermal mechanism is connected to the translation heating cavity and is used to maintain the temperature inside the translation heating cavity at a translation reaction temperature that allows the translation reaction to occur. The translation reaction module is disposed inside the translation heating cavity and has a translation reaction cavity for performing the translation reaction.
[0019] Preferably, a third guide slide is provided inside the translation heating cavity, through which the translation reaction module can enter and exit the translation heating cavity.
[0020] Preferably, the second electrothermal mechanism is disposed at the bottom of the transcription heating chamber.
[0021] In some more specific implementations, the translation observation module includes a translation observation cavity and a translation observation light source. The translation reaction module is disposed inside the translation observation cavity, and the translation observation light source is connected to the translation observation cavity. The translation observation light source is used to provide illumination conditions for observing the translation reaction.
[0022] Preferably, a fourth guide slide is provided inside the translation observation cavity, through which the translation reaction module can enter and exit the translation observation cavity.
[0023] Preferably, the translation observation light source is located at the bottom of the translation observation cavity.
[0024] In some more specific embodiments, the top of the reagent kit is provided with a translation observation window, which is a transparent viewing window located above the translation observation cavity, for observing the translation process within the translation response module.
[0025] Preferably, the translation observation module further includes a translation filter unit, which is disposed on the top of the translation observation cavity and covers the translation observation window.
[0026] In some more specific implementations, the teaching kit for demonstrating transcription and translation results in stages further includes a heat insulation unit. The translation heating module and the translation observation module are connected along a first direction, and the heat insulation unit is located between the translation heating module and the translation observation module along a second direction, and can switch between a first position and a second position along the second direction. The first direction is perpendicular to the second direction.
[0027] When the heat insulation unit is located at the first station, it is located outside the reagent kit body, allowing the translation heating module to communicate with the translation observation module. When the heat insulation unit is located at the second station, it is located inside the reagent kit body, isolating the translation heating module from the translation observation module.
[0028] In some more specific implementations, the control module includes a power supply, a first control circuit, a second control circuit, a third control circuit, and a fourth control circuit. The first control circuit, the second control circuit, the third control circuit, and the fourth control circuit are connected in parallel with the power supply. The first control circuit is connected in series with the transcription heating module, the second control circuit is connected in series with the transcription observation module, the third control circuit is connected in series with the translation heating module, and the fourth control circuit is connected in series with the translation observation module.
[0029] In some more specific implementations, the control module further includes a transcription heating switch, a transcription observation switch, a translation heating switch, and a translation observation switch. The transcription heating switch, transcription observation switch, translation heating switch, and translation observation switch are disposed on the surface of the reagent kit and are respectively connected to the first control circuit, the second control circuit, the third control circuit, and the fourth control circuit.
[0030] In a more preferred embodiment, the control module includes a fifth control circuit. The fifth control circuit is connected in series with the third control circuit. When the translation reaction switch is activated and opened, the heat insulation unit is located in the first position.
[0031] Compared with the prior art, the advantages of this utility model include at least the following:
[0032] First, the teaching kit for demonstrating transcription and translation results in stages provided by this invention includes a transcription heating module and a transcription observation module spaced apart inside the kit. Both the transcription heating and observation chambers have cylindrical guide channels to allow the transcription reaction tubes to be placed into the observation chamber for observation, thus enabling visualization of the entire process. The reaction chamber inside the transcription reaction tube utilizes capillary action to stabilize the liquid and provide a suitable reaction space. The transparent transcription reaction tubes facilitate real-time monitoring of fluorescence signal changes. The transcription heating chamber is made of metal to reduce friction, effectively conduct heat, and ensure a constant temperature environment.
[0033] Secondly, in the teaching kit for demonstrating transcription and translation results in stages provided by this utility model, the translation heating module and the translation observation module are connected along a first direction, and the heat insulation unit is located between the translation heating module and the translation observation module along a second direction. It can switch between a first position and a second position along the second direction. The heat insulation unit can effectively prevent heat transfer and ensure the temperature stability of the translation heating module and the translation observation module when conducting independent experiments. This not only improves the convenience of experimental operation, but also ensures the accuracy and reliability of experimental results. Attached Figure Description
[0034] Figure 1 This is a top view of a teaching kit for demonstrating transcription and translation results in stages, provided in this embodiment;
[0035] Figure 2 This is a schematic diagram of the structure of a teaching kit for demonstrating transcription and translation results in stages, provided in this embodiment;
[0036] Figure 3 This is a schematic diagram of the structure of a teaching kit for demonstrating transcription and translation results in stages, provided in this embodiment;
[0037] Figure 4 This is a schematic diagram of the structure of a teaching kit for demonstrating transcription and translation results in stages, provided in this embodiment;
[0038] Figure 5 This is a schematic diagram showing the connection between the transcription reaction tube and the translation reactor provided in this embodiment.
[0039] Explanation of reference numerals in the attached figures:
[0040] 1. Transcription heating module; 2. Transcription observation module; 3. Translation heating module; 4. Translation observation module; 11. Transcription reaction tube; 31. Translation reactor; 12. Transcription reaction chamber; 13. Transcription heating plate; 14. Transcription heating chamber; 15. Transcription heating switch; 21. Transcription observation lamp; 22. Transcription observation chamber; 23. Transcription filter; 24. Transcription observation switch; 32. Translation heating chamber; 33. Translation heating plate; 34. Translation heat insulation plate; 35. Translation heating switch; 41. Translation observation lamp; 42. Translation observation chamber; 43. Translation filter; 44. Translation observation switch. Detailed Implementation
[0041] In view of the shortcomings of the prior art, the inventor of this case, through long-term research and extensive practice, has come up with the technical solution of this utility model. The following will further explain the technical solution, its implementation process, and its principles.
[0042] Please refer to Figure 1 This invention provides a teaching kit for demonstrating transcription and translation results in stages, comprising a kit body, a control module, a transcription reaction tube 11, a translation reactor 31, and a transcription heating module 1, a transcription observation module 2, a translation heating module 3, and a translation observation module 4 arranged at intervals. The transcription reaction tube 11, translation reactor 31, transcription heating module 1, transcription observation module 2, translation heating module 3, translation observation module 4, and control module are all encapsulated within the kit body. The transcription reaction tube 11 can be transferred between the transcription heating module 1 and the transcription observation module 2. Specifically, when the transcription reaction heating stage is required, the transcription reaction tube 11 can be placed in the transcription heating module 1 to receive appropriate heating, ensuring that the transcription reaction proceeds smoothly at a suitable transcription reaction temperature. Once the transcription reaction is complete, the transcription reaction tube 11 can be transferred to the transcription observation module 2 for subsequent observation and analysis. The main function of the transcription heating module 1 is to precisely heat the transcription reaction tube 11, ensuring that the transcription reaction proceeds at the ideal transcription reaction temperature. The transcription observation module 2 is responsible for providing the necessary conditions and environment for observing the transcription reaction. After the transcription reaction is completed, the transcription reaction tube 11 is transferred to the transcription observation module 2, where the products of the transcription reaction can be observed and analyzed in detail.
[0043] Similarly, the translation reactor 31 can be transferred between the translation heating module 3 and the translation observation module 4. During the heating phase of the translation reaction, the translation reactor 31 can be placed in the translation heating module 3 to receive appropriate heating, ensuring the translation reaction proceeds smoothly at the suitable temperature. After the translation reaction is complete, the translation reactor 31 can be transferred to the translation observation module 4 for subsequent observation and analysis. The translation heating module 3 heats the translation reactor 31 to provide the necessary temperature for the translation reaction, ensuring it proceeds under optimal conditions. The translation observation module 4 provides the necessary conditions and environment for observing the translation reaction. After the translation reaction is complete, the translation reactor 31 is transferred to the translation observation module 4 for detailed observation and analysis of the translation reaction products.
[0044] Next, these modules will be described individually.
[0045] Please refer to Figures 2-3 In this scheme, the transcription heating module 1 includes a transcription heating chamber 14 and a transcription heating plate 13. The transcription heating plate 13 is located at the bottom of the transcription heating chamber 14. A cylindrical first guide slide is provided inside the transcription heating chamber 14, allowing the transcription reaction tube 11 to be flexibly and removably positioned within the transcription heating chamber 14 along this cylindrical first guide slide. The transcription reaction tube 11 is a hollow cylindrical structure with a reagent inlet on its wall for easy reagent addition, and its internal space is the transcription reaction chamber 12.
[0046] Specifically, the reaction chamber utilizes capillary action to stabilize the liquid within, ensuring it does not easily overflow or leak, while providing a suitable reaction space for the transcription reaction. Furthermore, the transcription reaction tube 11 is made of a transparent material, allowing for easy observation of changes in the fluorescence signal during the transcription reaction, thus enabling real-time monitoring and analysis of the reaction process. As for the transcription heating chamber 14, it is made of metal, which not only effectively reduces friction when the transcription reaction tube 11 is inserted, but also effectively conducts the heat generated by the transcription heating plate 13, ensuring a constant and suitable temperature environment for the transcription reaction.
[0047] In this design, the transcription observation module 2 includes a transcription observation chamber 22 and a transcription observation lamp 21. The transcription observation lamp 21 is located at the bottom of the transcription observation chamber 22. Furthermore, the transcription observation chamber 22 is designed with a second guide slide, allowing the transcription reaction tube 11 to be removably positioned within the transcription observation chamber 22 along the second guide slide for easy transcription observation. A transcription observation window is located on the top of the kit. The transcription observation window communicates with the transcription observation chamber 22, allowing users to directly observe the transcription process within the transcription observation chamber 22, thereby better monitoring the experiment.
[0048] Specifically, the transcription observation module 2 also includes a transcription filter 23. The transcription filter 23 is positioned on top of the transcription observation chamber 22 and covers the transcription observation window. It effectively filters out unwanted light, allowing only specific wavelengths of light to pass through, thereby improving the accuracy and clarity of the transcription process observation. The transcription observation lamp 21 uses an orange LED lamp with a specific excitation wavelength, which effectively excites the red fluorescence signal after the transcription reaction occurs, making the fluorescence signal during transcription more obvious and easier to observe. The transcription observation chamber 22 is made of transparent material, allowing the fluorescence signal inside the transcription reaction tube 11 to be clearly observed, improving the convenience of observation. The transcription filter 23 is a narrow-band red filter, which effectively transmits the red fluorescence of the positive signal while further eliminating the influence of background orange light, greatly improving the accuracy and reliability of the observation and ensuring the precision of the experimental data.
[0049] Please refer to Figures 4-5 In this design, the reaction heating module 3 includes a translation heating chamber 32 and a translation heating plate 33. The translation heating plate 33 is located at the bottom of the translation heating chamber 32 and is tightly connected to it, ensuring effective heat transfer. The translation heating chamber 32 has a third guide slide, allowing the translation reactor 31 to be flexibly and removably positioned within the translation heating chamber 32 along the third guide slide. The translation reactor 31 is made of transparent material. The transparent material allows direct observation of the reactor's interior, thus enabling better monitoring and analysis of the translation reaction process.
[0050] In this design, the translation observation module 4 includes a translation observation cavity 42, a translation observation lamp 41, and a translation filter 43. The translation observation cavity 42 provides an observation environment for the translation process. The translation observation lamp 41 is located at the bottom of the translation observation cavity 42. Furthermore, a fourth guide slide is provided inside the translation observation cavity 42, along which the translation reactor 31 can be placed into or removed from the translation observation cavity 42. A translation observation window is provided on the top of the kit in this design. The translation process inside the translation observation cavity 42 can be conveniently observed through the translation observation window, thereby monitoring the progress of the translation reaction in real time. The translation filter 43 is located on top of the translation observation cavity 42 and covers the translation observation window. The translation filter 43 has a dual function: on the one hand, it allows light of a specific wavelength to pass through, enabling the user to observe specific fluorescence signals generated during the translation process; on the other hand, it can effectively filter out light of other wavelengths, especially those that may interfere with the observation results, thereby improving the accuracy and clarity of the translation process observation. In this scheme, the translation observation lamp 41 uses a blue LED light, which can excite the green fluorescence of the translated green fluorescent protein to observe the occurrence of the reaction. The translation filter 43 uses an orange filter, which allows green fluorescence to pass through while effectively reducing the interference of blue excitation light on the observation results, ensuring the accuracy and reliability of the observation results.
[0051] In this scheme, the teaching kit for demonstrating transcription and translation results in stages also includes a translation heat shield 34. The translation heating chamber 32 and the translation observation chamber 42 are interconnected along a first direction. To ensure temperature control and safety during the experiment, the translation heat shield 34 is positioned between the translation heating chamber 32 and the translation observation chamber 42, and can be switched between a first position and a second position along a second direction perpendicular to the first direction.
[0052] Specifically, when the translation heat shield 34 is in the first position, i.e., outside the reagent kit, the translation heating chamber 32 and the translation observation chamber 42 are interconnected, allowing the translation reactor 31 to easily move between them, facilitating related experimental operations. When the translation heat shield 34 moves to the second position, i.e., inside the reagent kit, it effectively isolates the translation heating chamber 32 from the translation observation chamber 42. This isolation effectively prevents heat transfer, ensuring temperature stability of the translation heating chamber 32 and the translation observation chamber 42 during independent experiments. By cleverly using the translation heat shield 34, not only is the convenience of experimental operation improved, but the accuracy and reliability of experimental results are also guaranteed.
[0053] When the translation reactor 31 needs to enter the translation heating chamber 32, the translation heat shield 34 is first triggered to lift. At this time, the translation reactor 31 enters through the opening of the translation observation chamber 42 and moves to the right into the translation heating chamber 32. Once the translation reactor 31 is in place, the translation heat shield 34 is lowered, thus forming a sealed environment in the translation heating chamber 32. This sealed design is crucial for maintaining the specific reaction space required for the translation reaction, which helps to maintain the stability and accuracy of the reaction.
[0054] In this scheme, the control module includes a power supply, a first control circuit, a second control circuit, a third control circuit, and a fourth control circuit. The first control circuit, the second control circuit, the third control circuit, and the fourth control circuit are connected in parallel with the power supply. The first control circuit is connected in series with the transcription heating plate 13. The second control circuit is connected in series with the transcription observation lamp 21. The third control circuit is connected in series with the translation heating plate 33. The fourth control circuit is connected in series with the translation observation lamp 41.
[0055] In this solution, the control module further includes a transcription heating switch 15, a transcription observation switch 24, a translation heating switch 35, and a translation observation switch 44. The transcription heating switch 15, the transcription observation switch 24, the translation heating switch 35, and the translation observation switch 44 are disposed on the surface of the reagent kit and are respectively connected to the transcription heating plate 13, the transcription observation lamp 21, the translation heating plate 33, and the translation observation lamp 41.
[0056] Example
[0057] A teaching kit for demonstrating transcription and translation results in stages, comprising the following steps:
[0058] 1. Preparation stage:
[0059] Prepare the components and reagents required for the transcription and translation modules according to the instructions. The transcription module includes three different reagents, labeled A, B, and C. Reagent A contains the energy and buffer required for template transcription, as well as reagents for detecting the transcription results. Specifically, Reagent A consists of 40 mM Tris-HCl (pH 8.0), 6 mM magnesium chloride, 10 mM DTT, 50 mM sodium chloride, 2 mM spermidine, 0.5 mM NTP, and 1 U / µl T7 RNA polymerase. Additionally, 25 µM malachite green dye is added for easier observation of the transcription results. Reagent B is the linear template GFP required for transcription, characterized by the presence of a malachite green aptamer, which specifically binds to malachite green to form a three-dimensional structure, thus emitting red fluorescence. The specific sequence is SEQ1. Reagent C is a deionized reagent. The translation module is supplemented with reagent D, which comprises 57 mM HEPES-KOH buffer, 1.2 mM ATP, 0.85 mM UTP, 0.85 mM CTP, 0.85 mM GTP, 0.64 mM cAMP, 200 mM potassium glutamate, 80 mM ammonium acetate, 15 mM magnesium acetate, 2 mM amino acids, 2% PEG8000 (by weight), 33 mM phosphoenolpyruvate, and 25% E. coli extract (by volume). These components together form the basis of the translation module, providing the necessary conditions for protein synthesis. SEQ 1:
[0060]
[0061] 2. Transcription stage:
[0062] First, take a transcription reaction tube labeled NC and add reagent A and deionized water reagent C to it; these will serve as a negative control for the transcription reaction. Next, take another transcription reaction tube labeled Sample and add reagent A and reagent B to it; these will serve as the sample for the transcription reaction. Once ready, place both transcription reaction tubes together into the transcription heating chamber 14. Next, turn on the transcription heating switch 15, set the temperature to 37°C, and start the reaction. Allow the transcription reaction to proceed at the set temperature for 2 hours, then remove the reaction tubes and turn off the transcription heating switch 15 to stop heating. Subsequently, place the transcription reaction tubes into the transcription observation chamber 22, turn on the transcription observation switch 24, and observe under the illumination of the orange LED transcription observation lamp 21. During this process, you will notice that no fluorescence appears in the NC transcription reaction chamber 12, indicating that the negative control was successful. The Sample transcription reaction chamber 12 will display red light, indicating that the DNA template has been successfully transcribed into mRNA.
[0063] 3. Translation stage:
[0064] First, reagent D is added to translation reactor 31. Then, the transcription reaction chamber 12 is placed vertically within translation reactor 31, ensuring its stability. The liquid in the transcription reaction chamber 12 is uniformly transferred to translation reactor 31 by agitation, and thoroughly mixed. After mixing, the translation heat shield 34 is gently lifted so that translation reactor 31 can smoothly move through the opening of the reaction observation chamber to translation observation chamber 42, and slide to the right, eventually reaching the position of translation heating chamber 32. Once in the designated position, the translation heat shield 34 is lowered, ensuring that translation heating chamber 32 forms a completely sealed unit. At this point, the translation heating switch 35 is turned on, the temperature is set to 30°C, and the reaction is allowed to continue for 2 hours. After the time is up, translation reactor 31 is removed and translation heating switch 35 is turned off. Then, the translation heat shield 34 is lifted again, and translation reactor 31 is moved to the left, returning it to the position of translation observation chamber 42. At this point, turn on the translation observation switch 44 and observe the reaction of the NC liquid and Sample liquid on the translation reactor 31 under the illumination of the blue LED translation observation lamp 41. If the NC liquid does not fluoresce, while the Sample liquid shows a green glow, this means that the mRNA template has been successfully transcribed and protein has been generated.
[0065] It should be understood that the above embodiments are merely illustrative of the technical concept and features of this utility model, and are intended to enable those skilled in the art to understand the content of this utility model and implement it accordingly. They should not be construed as limiting the scope of protection of this utility model. All equivalent changes or modifications made in accordance with the spirit and essence of this utility model should be included within the scope of protection of this utility model.
Claims
1. A teaching kit for demonstrating transcription and translation results in stages, characterized in that, The kit includes a reagent kit, a control module, a transcription reaction module, a translation reaction module, a transcription heating module, a transcription observation module, a translation heating module, and a translation observation module. These modules are encapsulated within the reagent kit. The control module is connected to the transcription heating module, transcription observation module, translation heating module, and translation observation module, and is used to control the transcription and translation processes. The transcription reaction module can be transferred between the transcription heating module and the transcription observation module, and the translation reaction module can be transferred between the translation heating module and the translation observation module. The transcription heating module is used to heat the transcription reaction module to provide the transcription reaction temperature for the transcription reaction to occur, and the translation heating module is used to heat the translation reaction module to provide the translation reaction temperature for the translation reaction to occur. The transcription observation module is used to provide the observation conditions for observing the transcription reaction, and the translation observation module is used to provide the observation conditions for observing the translation reaction.
2. The teaching kit for demonstrating transcription and translation results in stages according to claim 1, characterized in that, The transcription heating module includes a transcription heating cavity and a first electrothermal mechanism. The transcription reaction module is disposed inside the transcription heating cavity. The transcription reaction module has a transcription reaction cavity for performing a transcription reaction. The first electrothermal mechanism is connected to the transcription heating cavity and is used to maintain the temperature inside the transcription heating cavity at a transcription reaction temperature that allows a transcription reaction to occur. And / or, a first guide slide is provided in the transcription heating chamber, and the transcription reaction module can enter and exit the transcription heating chamber through the first guide slide; And / or, the first electrothermal mechanism is disposed at the bottom of the transcription heating chamber.
3. The teaching kit for demonstrating transcription and translation results in stages according to claim 1, characterized in that, The transcription observation module includes a transcription observation chamber and a transcription observation light source. The transcription reaction module is disposed inside the transcription observation chamber, and the transcription observation light source is connected to the transcription observation chamber. The transcription observation light source is used to provide illumination conditions for observing the transcription reaction. And / or, a second guide slide is provided in the transcription observation chamber, and the transcription reaction module can enter and exit the transcription observation chamber through the second guide slide; And / or, the transcription observation light source is located at the bottom of the transcription observation chamber.
4. The teaching kit for demonstrating transcription and translation results in stages according to claim 3, characterized in that, The reagent kit has a transcription observation window on its top. This transcription observation window is transparent and visible, used to observe the transcription process within the transcription reaction module. And / or, the transcription observation module further includes a transcription filter unit disposed at the top of the transcription observation chamber and covering the transcription observation window.
5. The teaching kit for demonstrating transcription and translation results in stages according to claim 2, characterized in that, The translation heating module includes a translation heating cavity and a second electrothermal mechanism. The second electrothermal mechanism is connected to the translation heating cavity and is used to maintain the temperature inside the translation heating cavity at a translation reaction temperature that allows the translation reaction to occur. The translation reaction module is disposed inside the translation heating cavity and has a translation reaction cavity for performing the translation reaction. And / or, a third guide slide is provided inside the translation heating cavity, through which the translation reaction module can enter and exit the translation heating cavity; And / or, the second electrothermal mechanism is disposed at the bottom of the transcription heating chamber.
6. The teaching kit for demonstrating transcription and translation results in stages according to claim 1, characterized in that, The translation observation module includes a translation observation cavity and a translation observation light source. The translation reaction module is disposed inside the translation observation cavity, and the translation observation light source is connected to the translation observation cavity. The translation observation light source is used to provide illumination conditions for observing the translation reaction. And / or, a fourth guide slide is provided inside the translation observation cavity, and the translation reaction module can enter and exit the translation observation cavity through the fourth guide slide; And / or, the translation observation light source is located at the bottom of the translation observation cavity.
7. The teaching kit for demonstrating transcription and translation results in stages according to claim 6, characterized in that, The reagent kit has a translation observation window on its top. This window is transparent and allows observation of the translation process within the translation response module. And / or, the translation observation module further includes a translation filter unit, which is disposed on the top of the translation observation cavity and covers the translation observation window.
8. The teaching kit for demonstrating transcription and translation results in stages according to claim 1, characterized in that, It also includes a heat insulation unit. The translation heating module and the translation observation module are connected along a first direction. The heat insulation unit is located between the translation heating module and the translation observation module along a second direction and can switch between a first station and a second station along the second direction. The first direction and the second direction are perpendicular. When the heat insulation unit is located at the first station, it is located outside the reagent kit body, allowing the translation heating module to communicate with the translation observation module. When the heat insulation unit is located at the second station, it is located inside the reagent kit body, isolating the translation heating module from the translation observation module.
9. The teaching kit for demonstrating transcription and translation results in stages according to claim 1, characterized in that, The control module includes a power supply, a first control circuit, a second control circuit, a third control circuit, and a fourth control circuit. The first, second, third, and fourth control circuits are connected in parallel with the power supply. The first control circuit is connected in series with the transcription heating module, the second control circuit is connected in series with the transcription observation module, the third control circuit is connected in series with the translation heating module, and the fourth control circuit is connected in series with the translation observation module.
10. The teaching kit for demonstrating transcription and translation results in stages according to claim 9, characterized in that, The control module further includes a transcription heating switch, a transcription observation switch, a translation heating switch, and a translation observation switch. The transcription heating switch, transcription observation switch, translation heating switch, and translation observation switch are disposed on the surface of the reagent kit and are respectively connected to the first control circuit, the second control circuit, the third control circuit, and the fourth control circuit.