Fermentation feeding system for fusidic acid production

Abstract

The utility model discloses a fermentation feed supplement system for fusidic acid production, which relates to the technical field of fermentation feed supplement and comprises a feed supplement tank, a fermentation tank and a feed supplement cup, the feed supplement tank is connected with the feed supplement cup, the feed supplement cup is connected with the fermentation tank, and a peristaltic pump is arranged on a connecting pipeline of the feed supplement cup and the fermentation tank. Through cooperative operation of the material supplementing tank, the material supplementing cup, the flow sensor, the peristaltic pump and the like, accurate material supplementing can be realized according to dynamic requirements of microorganisms for nutrition in different fermentation stages of fusidic acid. The problem of overnutrition or insufficiency in a traditional material supplementing mode is effectively avoided, generation of by-products is reduced, microorganisms can obtain appropriate nutrients in all growth stages, a stable material basis is provided for efficient synthesis of fusidic acid, and then the yield and quality of products are improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to fermentation feed supplementing technical field especially relates to a fermentation feed supplementing system for fusidic acid production. BACKGROUND

[0002] Antibiotics play a pivotal role in the field of modern medicine and are the key drugs for treating various bacterial infectious diseases. As a highly effective and narrow-spectrum antibiotic, fusidic acid mainly targets gram-positive bacteria, especially drug-resistant Staphylococcus aureus, and has excellent antibacterial activity. It is widely used in the treatment of various diseases such as skin and soft tissue infections and osteomyelitis. Its industrial production is highly dependent on microbial fermentation technology. Microorganisms synthesize fusidic acid through a series of complex metabolic pathways in a suitable nutrient environment and physical conditions. Traditional fusidic acid fermentation processes are usually carried out in large fermentation tanks. As the core place for microbial growth and product synthesis, fermentation tanks provide a relatively closed and controllable environment for microorganisms.

[0003] In the early stage of fusidic acid fermentation, microorganisms are in a rapid growth phase, and have a large demand for carbon sources, nitrogen sources, and various growth factors. However, some simple feeding methods available today cannot accurately match the dynamic needs of microorganisms at this stage, often resulting in excessive initial nutrients, leading to rapid microbial growth, metabolic imbalance, and excessive by-products, which not only wastes raw materials but also may inhibit the subsequent synthesis of fusidic acid. Or in the middle and late stages of fermentation, the supply of key nutrients is insufficient, and the growth of microorganisms is limited, slowing down or even stopping the production of fusidic acid. For example, some factories use a fixed-time and fixed-quantity feeding strategy, which does not fully consider the changes in the absorption rate of nutrients by microorganisms during fermentation, and cannot adjust the composition and quantity of the feed in a timely manner according to the actual growth conditions, causing the nutrient composition in the fermentation broth to deviate from the optimal ratio, affecting the final yield and quality of fusidic acid.

[0004] Therefore, in view of the above phenomenon, a fermentation feed supplementing system for fusidic acid production is proposed to meet the needs of actual use. INVENTION CONTENTS

[0005] The utility model provides a fermentation feed supplementing system for fusidic acid production, solve the technical problem in the prior art.

[0006] To solve the above technical problems, the utility model provides a fermentation feed supplementing system for fusidic acid production, which comprises a feed tank, a fermentation tank and a feed cup, the feed tank is connected with the feed cup, the feed cup is connected with the fermentation tank, and a peristaltic pump is arranged on the connecting pipeline of the feed cup and the fermentation tank.

[0007] In some embodiments, the feed tank is connected with an air pressure pump, and a third electromagnetic valve is arranged on the connecting pipeline of the feed tank and the feed cup.

[0008] In some embodiments, a balance pipe is further connected between the feeding cup and the fermentation tank, and a second electromagnetic valve is arranged on the balance pipe.

[0009] In some embodiments, a first electromagnetic valve is arranged on the connecting pipeline between the peristaltic pump and the fermentation tank.

[0010] In some embodiments, a flow sensor is arranged on the connecting pipeline between the feeding cup and the peristaltic pump.

[0011] In some embodiments, a pressure gauge, a thermocouple thermometer, a pH electrode and a dissolved oxygen electrode are arranged on the fermentation tank.

[0012] In some embodiments, a liquid level meter is arranged on the feeding tank.

[0013] In some embodiments, a scale line is arranged on the feeding cup.

[0014] Compared with the related art, the fermentation feeding system for fuscic acid production has the following advantages

[0015] Advantages:

[0016] The fermentation feeding system for fuscic acid production provided by the utility model realizes accurate feeding according to the dynamic demand of microorganisms for nutrients in different stages of fuscic acid fermentation through the cooperative operation of the feeding tank, the feeding cup, the flow sensor and the peristaltic pump and the like. The problem of excess or insufficient nutrients in the traditional feeding mode is effectively avoided, the generation of by-products is reduced, the microorganisms can obtain suitable nutrients in each growth stage, a stable material basis is provided for the efficient synthesis of fuscic acid, and the product yield and quality are improved.

[0017] The fermentation feeding system for fuscic acid production provided by the utility model, the balance pipe and the related electromagnetic valve are arranged, the air pressure between the feeding cup and the fermentation tank can be balanced during feeding, and the fluctuations of the fermentation liquid temperature, the dissolved oxygen and the pH value caused by the air pressure difference are prevented. The peristaltic pump accurately controls the feeding flow rate, the opening and closing of the electromagnetic valve are matched, the feeding process is ensured to be stable and orderly, the interference on the stable environment formed in the fermentation tank is reduced to the maximum extent, the microorganisms are ensured to be always in suitable growth and metabolism conditions, and the continuous and stable synthesis of fuscic acid is facilitated. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 It is a whole structure schematic view of the utility model.

[0019] Marked numbers in the drawing: 1, feeding tank; 2, fermentation tank; 3, feeding cup; 4, peristaltic pump; 5, flow sensor; 6, feeding pipe; 7, balance pipe; 8, first electromagnetic valve; 9, second electromagnetic valve; 10, third electromagnetic valve; 11, air pressure pump. Detailed Implementation

[0020] Example 1

[0021] This embodiment provides a fermentation feeding system for fusidic acid production, such as... Figure 1 As shown, this invention installs a pressure gauge, a thermocouple thermometer, a pH electrode, and a dissolved oxygen electrode on the fermenter 2. The pressure gauge is installed on the top or side of the fermenter to monitor the pressure inside the tank in real time, ensuring the fermentation process is within a suitable pressure range and preventing adverse effects on microbial growth and product synthesis due to excessively high or low pressure. The thermocouple thermometer is inserted into the fermenter to accurately measure the temperature of the fermentation broth, as fusidic acid fermentation is highly temperature-sensitive and requires precise control within a certain range to ensure the metabolic activity of microorganisms and the efficiency of product synthesis. The pH electrode and dissolved oxygen electrode also need to be properly installed inside the fermenter. The pH electrode monitors the acidity or alkalinity of the fermentation broth; different microorganisms have specific pH requirements during fermentation, and a suitable pH range contributes to the efficient synthesis of fusidic acid. The dissolved oxygen electrode monitors the dissolved oxygen content in the fermentation broth; the dissolved oxygen requirements of fusidic acid fermenting microorganisms vary at different growth stages, and monitoring dissolved oxygen allows for better adjustment of fermentation conditions.

[0022] A level gauge, either an ultrasonic or hydrostatic level gauge, is installed on the feeding tank 1 to accurately measure the liquid level inside the tank, ensuring timely monitoring of the remaining feeding amount and guaranteeing the continuity and stability of the feeding process. The level gauge feeds back the liquid level information to the control system, allowing operators to replenish materials promptly when the level falls below the set value. An air pump 11 is connected to the feeding tank 1 to provide stable air pressure, propelling the feed from the tank to the feeding cup 3. Adjusting the air pressure of the pump controls the flow rate and volume of the feed, meeting the feeding needs at different fermentation stages. Simultaneously, a third solenoid valve 10 is installed on the connecting pipeline between the feeding tank 1 and the feeding cup 3. This valve is controlled by the control system, opening or closing as needed to precisely control the feed delivery.

[0023] A scale is provided on the feeding cup 3, allowing operators to visually observe the amount of material inside and assist in precise control of the feeding amount. A flow sensor 5 is installed on the connecting pipe between the feeding cup 3 and the peristaltic pump 4. The flow sensor 5 can monitor the flow rate of the feeding material flowing out of the feeding cup 3 in real time and transmit the flow data to the control system for precise control of the feeding rate and total amount. The feeding cup 3 is connected to the fermentation tank 2 via a pipeline, on which the peristaltic pump 4 and a first solenoid valve 8 are installed. The peristaltic pump 4 is a precision fluid conveying device. Its working principle is to use the rotation of rollers to squeeze the elastic hose, pushing the fluid in the tube forward. It has precise flow control and good sealing performance, ensuring accurate and stable delivery of the feeding material into the fermentation tank 2. The first solenoid valve 8 works in conjunction with the peristaltic pump 4, and the feeding flow path can be flexibly opened or closed through the control system to achieve automated control of the feeding process. A balance pipe 7 is connected between the feeding cup 3 and the fermentation tank 2, and a second solenoid valve 9 is installed on the balance pipe 7. The function of the balancing pipe 7 is to balance the air pressure between the feeding cup 3 and the fermentation tank 2. When feeding is performed, the second solenoid valve 9 is opened to keep the air pressure between the two balanced, so as to avoid feeding abnormalities caused by air pressure difference and ensure the smooth progress of the feeding process.

[0024] Implementation steps:

[0025] I. System Initialization and Preparation:

[0026] Before commencing fusidic acid fermentation, the entire system is inspected to ensure all components are correctly installed, connections are tight, and there are no leaks. Fermentation tank 2 is cleaned and sterilized, for example, using high-temperature steam sterilization to kill any potential contaminants and provide a sterile environment for fusidic acid fermentation. Simultaneously, feed tank 1 and feed cup 3 are cleaned and disinfected to ensure the feed system is free from impurities or microbial contamination.

[0027] According to the culture medium formula required for fusidic acid fermentation, add an appropriate amount of fermentation culture medium to fermenter 2, including glucose, soybean flour, corn steep liquor, sunflower oil, butyl acetate, sulfuric acid, methanol, etc., and adjust the initial parameters such as pH value, temperature and dissolved oxygen concentration to create a suitable initial environment for microbial growth and fusidic acid synthesis.

[0028] Add the required feed material to feed tank 1, ensuring that the purity and concentration of the feed material meet the requirements, and check whether the liquid level in the feed tank is sufficient according to the liquid level gauge to ensure the feed supply throughout the fermentation process.

[0029] II. Feeding operations during fermentation:

[0030] During the fermentation process, according to the data feedback from the sensors on the fermenter 2 (thermocouple thermometer, pH electrode, dissolved oxygen electrode), combined with the needs of different stages of fusidic acid fermentation, the control system determines whether it needs to be fed.

[0031] When feeding is needed, first open the third electromagnetic valve 10, start the air pressure pump 11, and transport the feed from the feed tank 1 to the feed cup 3. During the feeding process, the amount of feed flowing into the feed cup 3 is monitored by the flow sensor 5, and when the preset amount is reached, the third electromagnetic valve 10 and the air pressure pump 11 are closed.

[0032] Then open the first electromagnetic valve 8 and the peristaltic pump 4, and the peristaltic pump 4 transports the feed in the feed cup 3 to the fermenter 2 at a set flow rate. At the same time, open the second electromagnetic valve 9 to balance the air pressure between the feed cup 3 and the fermenter 2 through the balance pipe 7. The flow rate of the feed is continuously monitored by the flow sensor 5, and according to the fermentation requirements, the control system can adjust the speed of the peristaltic pump 4 in real time to accurately control the amount and flow rate of the feed.

[0033] For example, during the logarithmic growth phase of fusidic acid fermentation, the demand for nutrients by microorganisms increases rapidly, at which time the control system increases the speed of the peristaltic pump 4 according to the pre-set program to increase the amount of feed to meet the growth needs of the microorganisms; while in the stationary phase, according to the data of the dissolved oxygen electrode in the fermentation broth, if the dissolved oxygen decreases, it may be because the microorganisms grow vigorously and consume too much oxygen, so the composition or flow rate of the feed can be adjusted appropriately to maintain a suitable dissolved oxygen level.

Claims

1. A fermentation feed system for the production of fusidic acid, characterized in that: Including feeding tank, fermenter and feeding cup, the feeding tank is connected with the feeding cup, the feeding cup is connected with the fermenter, and a peristaltic pump is arranged on the connecting pipeline of the feeding cup and the fermenter.

2. The fermentation feed system for producing fusidic acid according to claim 1, wherein, The feeding tank is connected with an air pressure pump, and a third electromagnetic valve is arranged on the connecting pipeline of the feeding tank and the feeding cup.

3. The fermentation feed system for producing fusidic acid according to claim 1, wherein, A balance pipe is further connected between the feeding cup and the fermenter, and a second electromagnetic valve is arranged on the balance pipe.

4. The fermentation feed system for producing fusidic acid according to claim 1, wherein, A first electromagnetic valve is arranged on the connecting pipeline of the peristaltic pump and the fermenter.

5. The fermentation feed system for producing fusidic acid according to claim 1, wherein, A flow sensor is arranged on the connecting pipeline of the feeding cup and the peristaltic pump.

6. The fermentation feed system for producing fusidic acid according to claim 1, wherein, A pressure gauge, a thermocouple thermometer, a PH electrode and a dissolved oxygen electrode are arranged on the fermenter.

7. The fermentation feed system for producing fusidic acid according to claim 1, wherein, A liquid level meter is arranged on the feeding tank.

8. The fermentation feed system for producing fusidic acid according to claim 1, wherein, Scale lines are arranged on the feeding cup.

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