Umbilical cord mesenchymal stem cell collecting and extracting device

Abstract

The utility model relates to the technical field of stem cell collection and extraction devices, in particular to an umbilical cord mesenchymal stem cell collection and extraction device which is characterized in that a stirring mechanism is started to stir crushed umbilical cord tissues and a stem cell decomposition solution, so that stem cells in the umbilical cord tissues are fully decomposed, and the umbilical cord mesenchymal stem cells are collected and extracted. The stem cell solution is discharged into the separating mechanism through the discharging mechanism, stem cells in the stem cell solution are separated by starting the separating mechanism, the solution is discharged through the discharging mechanism, and the practicability of the equipment is improved; comprising a feeding mechanism; the device further comprises a crushing mechanism, a stirring mechanism, a separating mechanism, a discharging mechanism and a cleaning mechanism, the crushing mechanism and the stirring mechanism are both installed in the feeding mechanism, the stirring mechanism is located below the crushing mechanism, the discharging mechanism is located on the right side of the feeding mechanism and installed in the separating mechanism, and the cleaning mechanism is installed on the left side of the feeding mechanism.

Description

Technical Field

[0001] This utility model relates to the technical field of stem cell collection and extraction devices, and in particular to a device for collecting and extracting umbilical cord mesenchymal stem cells. Background Technology

[0002] Umbilical cord mesenchymal stem cells (UCSCs) are mesenchymal stem cells derived from the umbilical cord tissue of newborns. These stem cells possess self-renewal capacity and multi-lineage differentiation potential, and can differentiate into various cell types under specific conditions, including osteocytes, chondrocytes, adipocytes, muscle cells, and nerve cells. They are not only found in the umbilical cord but may also exist in the connective tissue surrounding the umbilical cord vessels. Umbilical cord UCSCs possess a variety of biological characteristics, such as immune regulation, anti-inflammation, angiogenesis promotion, and tissue repair. These characteristics make them promising for applications in regenerative medicine, cell therapy, and tissue engineering.

[0003] For example, the stem cell collection and extraction device disclosed in the utility model patent with application number 202323019038.7 represents a class of prior art whose main structure includes a fixed handle, a guide rod, a guide groove, a sealing diaphragm, a first connecting rod, a reset spring, and a squeezing roller. Stem cell collection and extraction are achieved through the cooperation of the fixed handle, guide rod, guide groove, sealing diaphragm, first connecting rod, reset spring, and squeezing roller.

[0004] The stem cell extraction device in the aforementioned patent is difficult to fully extract stem cells and difficult to separate stem cells from the solution. Utility Model Content

[0005] To solve the above-mentioned technical problems, this utility model provides an umbilical cord mesenchymal stem cell collection and extraction device that uses a stirring mechanism to stir the broken umbilical cord tissue and stem cell decomposition solution, thereby fully decomposing the stem cells in the umbilical cord tissue. The stem cell solution is then discharged into a separation mechanism via a discharge mechanism. The stem cells in the stem cell solution are separated by activating the separation mechanism, and the solution is discharged through the discharge mechanism, thus improving the practicality of the equipment.

[0006] This utility model discloses an umbilical cord mesenchymal stem cell collection and extraction device, including a feeding mechanism; it also includes a crushing mechanism, a stirring mechanism, a separation mechanism, a discharge mechanism, and a cleaning mechanism. The crushing and stirring mechanisms are both installed inside the feeding mechanism, with the stirring mechanism located below the crushing mechanism. The discharge mechanism is located to the right of the feeding mechanism and installed inside the separation mechanism. The cleaning mechanism is installed to the left of the feeding mechanism. Umbilical cord tissue and stem cell decomposition solution are discharged into the feeding mechanism. The crushing mechanism is activated to crush the umbilical cord tissue, and the stirring mechanism is activated to stir the crushed umbilical cord tissue and stem cell decomposition solution, so that the stem cells in the umbilical cord tissue are fully decomposed. The feeding mechanism filters the umbilical cord tissue in the stem cell decomposition solution, and the stem cell solution is discharged into the separation mechanism through the discharge mechanism. The separation mechanism is activated to separate the stem cells in the stem cell solution, and the solution is discharged through the discharge mechanism. The cleaning mechanism is activated to disinfect and clean the inside of the equipment, improving the practicality of the equipment.

[0007] Preferably, the feeding mechanism includes a feeding hopper, a decomposition tank, a first filter plate, a second filter plate, a discharge hopper, a bent pipe, and multiple first support columns. The output end of the feeding hopper is connected to the top of the decomposition tank. The first and second filter plates are respectively installed on the inner wall of the decomposition tank, with the second filter plate located below the first filter plate. The input end of the discharge hopper is connected to the bottom of the decomposition tank, and the input end of the bent pipe is connected to the output end of the discharge hopper. The side ends of the multiple first support columns are respectively connected to the outer wall of the decomposition tank. Umbilical cord tissue and stem cell decomposition solution are discharged into the decomposition tank through the feeding hopper. The first filter plate blocks unbroken umbilical cord tissue, and the second filter plate filters the decomposed umbilical cord tissue. The stem cell solution is discharged into the discharge mechanism through the discharge hopper and the bent pipe. The multiple first support columns support the decomposition tank, improving the practicality of the equipment.

[0008] Preferably, the crushing mechanism includes a first motor, a crushing shaft, and multiple crushing blades. The top of the crushing shaft is mounted on the output end of the first motor, and multiple crushing blades are provided on the crushing shaft. By starting the first motor, the crushing shaft is rotated, and the umbilical cord tissue is crushed by the multiple crushing blades, thereby improving the practicality of the equipment.

[0009] Preferably, the stirring mechanism includes a second motor, a stirring shaft, and multiple stirring blades. The bottom end of the stirring shaft is mounted on the output end of the second motor, and the multiple stirring blades are respectively mounted on the stirring shaft. By starting the second motor, the stirring shaft is rotated, and the multiple stirring blades stir the broken umbilical cord tissue and the stem cell decomposition solution, so that the stem cells in the umbilical cord tissue are fully separated, thereby improving the practicality of the equipment.

[0010] Preferably, the separation mechanism includes a third motor, multiple connecting rods, a separation tank, a discharge pipe, and bearings. The side ends of the multiple connecting rods are respectively installed on the output end of the third motor, and the other ends of the multiple connecting rods are respectively installed on the upper part of the inner wall of the separation tank. The input end of the discharge pipe is connected to the bottom end of the separation tank, and a valve is provided on the output end of the discharge pipe. The inner ring of the bearing is installed on the outer wall of the discharge pipe, and the outer ring of the bearing is installed on the discharge mechanism. By starting the third motor and connecting it through the multiple connecting rods, the connecting rods are rotated to separate the solution in the stem cells. The stem cells in the separation tank are discharged through the discharge pipe, improving the practicality of the equipment.

[0011] Preferably, the discharge mechanism includes a discharge tank, a discharge pipe, and multiple second support columns. The input end of the discharge pipe is connected to the outer wall of the discharge tank, and a valve is provided on the output end of the discharge pipe. The bottom end of the discharge tank is installed on the top of the multiple second support columns. The cell decomposition solution in the discharge tank is discharged through the discharge pipe, and the multiple second support columns support the discharge tank respectively, thereby improving the practicality of the equipment.

[0012] Preferably, the cleaning mechanism includes a first pipe, a cleaning pump, a second pipe, and a support. The output end of the first pipe is connected to the input end of the cleaning pump, and the output end of the cleaning pump is connected to the input end of the second pipe. A valve is installed on the output end of the second pipe, and the bottom end of the cleaning pump is installed on the top of the support. The output end of the cleaning liquid is connected to the input end of the first pipe. By starting the cleaning pump, the cleaning liquid is discharged into the feeding mechanism through the second pipe to clean the inner wall of the equipment. The support supports the cleaning pump, improving the practicality of the equipment.

[0013] Compared with the prior art, the beneficial effects of this utility model are as follows: umbilical cord tissue and stem cell decomposition solution are discharged into the feeding mechanism, the umbilical cord tissue is crushed by activating the crushing mechanism, and the crushed umbilical cord tissue and stem cell decomposition solution are stirred by activating the stirring mechanism, so that the stem cells in the umbilical cord tissue are fully decomposed. The umbilical cord tissue in the stem cell decomposition solution is filtered by the feeding mechanism, and the stem cell solution is discharged into the separation mechanism by the discharge mechanism. The stem cells in the stem cell solution are separated by activating the separation mechanism, and the solution is discharged by the discharge mechanism. The cleaning mechanism is activated to disinfect and clean the inside of the equipment, thereby improving the practicality of the equipment. Attached Figure Description

[0014] Figure 1 This is an isometric sectional view of the present invention;

[0015] Figure 2 This is an isometric sectional view of the feeding mechanism of this utility model;

[0016] Figure 3 This is an isometric schematic diagram of the crushing mechanism of this utility model;

[0017] Figure 4This is an isometric schematic diagram of the stirring mechanism of this utility model;

[0018] Figure 5 This is an isometric schematic diagram of the separation mechanism of this utility model;

[0019] Figure 6 This is an isometric schematic diagram of the discharge mechanism of this utility model;

[0020] Figure 7 This is an isometric schematic diagram of the cleaning mechanism of this utility model.

[0021] The attached diagram is labeled as follows: 01, feeding mechanism; 11, feeding hopper; 12, decomposition tank; 13, first filter plate; 14, second filter plate; 15, discharge hopper; 16, bent pipe; 17, first support column; 02, crushing mechanism; 21, first motor; 22, crushing shaft; 23, crushing blade; 03, stirring mechanism; 31, second motor; 32, stirring shaft; 33, stirring blade; 04, separation mechanism; 41, third motor; 42, connecting rod; 43, separation tank; 44, discharge pipe; 45, bearing; 05, discharge mechanism; 51, drain tank; 52, drain pipe; 53, second support column; 06, cleaning mechanism; 61, first pipe; 62, cleaning pump; 63, second pipe; 64, bracket. Detailed Implementation

[0022] To facilitate understanding of this utility model, a more complete description will be given below with reference to the accompanying drawings. This utility model can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to make the disclosure of this utility model more thorough and complete.

[0023] Example 1

[0024] like Figure 1 As shown, an umbilical cord mesenchymal stem cell collection and extraction device includes a feeding mechanism 01; it also includes a crushing mechanism 02, a stirring mechanism 03, a separation mechanism 04, a discharge mechanism 05, and a cleaning mechanism 06. The crushing mechanism 02 and the stirring mechanism 03 are both installed inside the feeding mechanism 01. The stirring mechanism 03 is located below the crushing mechanism 02. The discharge mechanism 05 is located on the right side of the feeding mechanism 01 and is installed inside the separation mechanism 04. The cleaning mechanism 06 is installed on the left side of the feeding mechanism 01.

[0025] The umbilical cord tissue and stem cell decomposition solution are fed into the feeding mechanism 01. The umbilical cord tissue is crushed by starting the crushing mechanism 02. The crushed umbilical cord tissue and stem cell decomposition solution are stirred by starting the stirring mechanism 03 to fully decompose the stem cells in the umbilical cord tissue. The umbilical cord tissue in the stem cell decomposition solution is filtered by the feeding mechanism 01. The stem cell solution is discharged into the separation mechanism 04 through the discharge mechanism 05. The stem cells in the stem cell solution are separated by starting the separation mechanism 04. The solution is discharged through the discharge mechanism 05. The cleaning mechanism 06 is started to disinfect and clean the inside of the equipment to improve the practicality of the equipment.

[0026] like Figure 2 As shown, the feeding mechanism 01 includes a feeding hopper 11, a decomposition barrel 12, a first filter plate 13, a second filter plate 14, a discharge hopper 15, a bent pipe 16, and multiple first support columns 17. The output end of the feeding hopper 11 is connected to the top of the decomposition barrel 12. The first filter plate 13 and the second filter plate 14 are respectively installed on the inner wall of the decomposition barrel 12, and the second filter plate 14 is located below the first filter plate 13. The input end of the discharge hopper 15 is connected to the bottom end of the decomposition barrel 12. The input end of the bent pipe 16 is connected to the output end of the discharge hopper 15. The side ends of the multiple first support columns 17 are respectively connected to the outer wall of the decomposition barrel 12.

[0027] like Figure 3 As shown, the crushing mechanism 02 includes a first motor 21, a crushing shaft 22 and a plurality of crushing blades 23. The top end of the crushing shaft 22 is mounted on the output end of the first motor 21, and a plurality of crushing blades 23 are provided on the crushing shaft 22.

[0028] like Figure 4 As shown, the stirring mechanism 03 includes a second motor 31, a stirring shaft 32, and multiple stirring blades 33. The bottom end of the stirring shaft 32 is mounted on the output end of the second motor 31, and the multiple stirring blades 33 are respectively mounted on the stirring shaft 32.

[0029] Umbilical cord tissue and stem cell decomposition solution are discharged into decomposition tank 12 through feed hopper 11. First filter plate 13 blocks unbroken umbilical cord tissue, and second filter plate 14 filters the decomposed umbilical cord tissue. Stem cell solution is discharged into discharge mechanism 05 through discharge hopper 15 and bend pipe 16. Multiple first support columns 17 support decomposition tank 12 respectively. By starting first motor 21, crushing shaft 22 is rotated, and multiple crushing blades 23 crush the umbilical cord tissue respectively. By starting second motor 31, stirring shaft 32 is rotated, and multiple stirring blades 33 stir the crushed umbilical cord tissue and stem cell decomposition solution respectively, so that the stem cells in the umbilical cord tissue are fully separated, improving the practicality of the equipment.

[0030] Example 2

[0031] like Figure 5 and Figure 6 As shown, based on Embodiment 1, it also includes a separation mechanism 04 and a discharge mechanism 05. The separation mechanism 04 includes a third motor 41, multiple connecting rods 42, a separation tank 43, a discharge pipe 44, and a bearing 45. The side ends of the multiple connecting rods 42 are respectively installed on the output end of the third motor 41, and the other ends of the multiple connecting rods 42 are respectively installed on the upper part of the inner wall of the separation tank 43. The input end of the discharge pipe 44 is connected to the bottom end of the separation tank 43, and a valve is provided on the output end of the discharge pipe 44. The inner ring of the bearing 45 is installed on the outer wall of the discharge pipe 44, and the outer ring of the bearing 45 is installed on the discharge mechanism 05. The discharge mechanism 05 includes a drain tank 51, a drain pipe 52, and multiple second support columns 53. The input end of the drain pipe 52 is connected to the outer wall of the drain tank 51, and a valve is provided on the output end of the drain pipe 52. The bottom end of the drain tank 51 is installed on the top end of the multiple second support columns 53.

[0032] By starting the third motor 41, which is connected to multiple connecting rods 42, the connecting rods 42 are rotated to separate the solution inside the stem cells. The stem cells in the separation tank 43 are discharged through the discharge pipe 44, and the cell decomposition solution in the drainage tank 51 is discharged through the drainage pipe 52. Multiple second support columns 53 support the drainage tank 51 respectively, improving the practicality of the equipment.

[0033] Example 3

[0034] like Figure 7 As shown, based on Embodiment 1, a cleaning mechanism 06 is also included. The cleaning mechanism 06 includes a first pipe 61, a cleaning pump 62, a second pipe 63, and a bracket 64. The output end of the first pipe 61 is connected to the input end of the cleaning pump 62, and the output end of the cleaning pump 62 is connected to the input end of the second pipe 63. A valve is provided on the output end of the second pipe 63, and the bottom end of the cleaning pump 62 is installed on the top end of the bracket 64.

[0035] The output end of the cleaning fluid is connected to the input end of the first pipe 61. By starting the cleaning pump 62, the cleaning fluid is discharged into the feeding mechanism 01 through the second pipe 63 to clean the inner wall of the equipment. The bracket 64 supports the cleaning pump 62 to improve the practicality of the equipment.

[0036] like Figures 1 to 7As shown, this utility model discloses an umbilical cord mesenchymal stem cell collection and extraction device. During operation, umbilical cord tissue and stem cell decomposition solution are first discharged into a decomposition tank 12 via a feed hopper 11. A first filter plate 13 blocks unbroken umbilical cord tissue, and a second filter plate 14 filters the decomposed umbilical cord tissue. The stem cell solution is discharged into a discharge mechanism 05 via a discharge hopper 15 and a curved pipe 16. Multiple first support columns 17 support the decomposition tank 12. Then, the first motor 21 is started to rotate the crushing shaft 22, which crushes the umbilical cord tissue using multiple crushing blades 23. Afterward, the second motor 31 is started to rotate the stirring shaft 32, which crushes the umbilical cord tissue using multiple stirring blades 33. The fragmented umbilical cord tissue and stem cell decomposition solution are stirred to fully separate the stem cells in the umbilical cord tissue. Then, by starting the third motor 41 connected to multiple connecting rods 42, the connecting rods 42 are rotated to separate the solution from the stem cells. The stem cells in the separation tank 43 are discharged through the discharge pipe 44. Then, the cell decomposition solution in the drainage tank 51 is discharged through the drainage pipe 52. Multiple second support columns 53 support the drainage tank 51 respectively. Finally, the output end of the cleaning liquid is connected to the input end of the first pipe 61. By starting the cleaning pump 62, the cleaning liquid is discharged into the feeding mechanism 01 through the second pipe 63 to clean the inner wall of the equipment. The bracket 64 supports the cleaning pump 62 to improve the practicality of the equipment.

[0037] The first motor 21, the second motor 31, the third motor 41, and the cleaning pump 62 of this utility model are commercially available. Technical personnel in this industry only need to install and operate them according to the accompanying instruction manual, without requiring any creative work from those skilled in the art.

[0038] The main function achieved by this utility model is: by activating the stirring mechanism 03 to stir the broken umbilical cord tissue and stem cell decomposition solution, the stem cells in the umbilical cord tissue are fully decomposed, and the stem cell solution is discharged into the separation mechanism 04 through the discharge mechanism 05. The stem cells in the stem cell solution are separated by activating the separation mechanism 04, and the solution is discharged through the discharge mechanism 05, thereby improving the practicality of the equipment.

[0039] The above description is only a preferred embodiment of the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. A device for collecting and extracting umbilical cord mesenchymal stem cells, comprising a feeding mechanism (01); characterized in that, It also includes crushing mechanism (02), stirring mechanism (03), separation mechanism (04), discharge mechanism (05) and cleaning mechanism (06), crushing mechanism (02) and stirring mechanism (03) are installed in feeding mechanism (01), stirring mechanism (03) is below crushing mechanism (02), discharge mechanism (05) is on the right side of feeding mechanism (01), discharge mechanism (05) is installed inside separation mechanism (04), cleaning mechanism (06) is installed on the left side of feeding mechanism (01).

2. The device for collecting and extracting umbilical cord mesenchymal stem cells according to claim 1, wherein, Feeding mechanism (01) includes feeding hopper (11), decomposition bucket (12), first filter plate (13), second filter plate (14), discharge hopper (15), elbow pipe (16) and multiple first support columns (17), the output end of feeding hopper (11) is connected with the top end of decomposition bucket (12), first filter plate (13) and second filter plate (14) are installed on the inner wall of decomposition bucket (12) respectively, and second filter plate (14) is below first filter plate (13), the input end of discharge hopper (15) is connected with the bottom end of decomposition bucket (12), the input end of elbow pipe (16) is connected with the output end of discharge hopper (15), and the side ends of multiple first support columns (17) are connected with the outer wall of decomposition bucket (12) respectively.

3. The device of claim 1, wherein the device is configured to collect and extract umbilical cord mesenchymal stem cells. Crushing mechanism (02) includes first motor (21), crushing shaft (22) and multiple crushing knives (23), the top end of crushing shaft (22) is installed on the output end of first motor (21), and multiple crushing knives (23) are arranged on crushing shaft (22).

4. The apparatus according to claim 1, wherein the apparatus is configured to collect the umbilical cord mesenchymal stem cells. Stirring mechanism (03) includes second motor (31), stirring shaft (32) and multiple stirring blades (33), the bottom end of stirring shaft (32) is installed on the output end of second motor (31), and multiple stirring blades (33) are installed on stirring shaft (32) respectively.

5. The apparatus according to claim 1, wherein the apparatus is configured to collect and extract umbilical cord mesenchymal stem cells. Separation mechanism (04) includes third motor (41), multiple connecting rods (42), separation bucket (43), discharge pipe (44) and bearing (45), the side ends of multiple connecting rods (42) are installed on the output end of third motor (41) respectively, the other ends of multiple connecting rods (42) are installed on the inner wall of separation bucket (43) respectively, the input end of discharge pipe (44) is connected with the bottom end of separation bucket (43), a valve is arranged on the output end of discharge pipe (44), the inner ring of bearing (45) is installed on the outer wall of discharge pipe (44), and the outer ring of bearing (45) is installed on discharge mechanism (05).

6. The umbilical cord mesenchymal stem cell collection and extraction device of claim 1, wherein, Discharge mechanism (05) includes liquid discharge bucket (51), liquid discharge pipe (52) and multiple second support columns (53), the input end of liquid discharge pipe (52) is connected with the outer wall of liquid discharge bucket (51), a valve is arranged on the output end of liquid discharge pipe (52), and the bottom end of liquid discharge bucket (51) is installed on the top end of multiple second support columns (53).

7. The apparatus according to claim 1, wherein the apparatus is configured to collect and extract umbilical cord mesenchymal stem cells. The cleaning mechanism (06) comprises a first pipeline (61), a cleaning pump (62), a second pipeline (63) and a bracket (64), the output end of the first pipeline (61) is connected with the input end of the cleaning pump (62), the output end of the cleaning pump (62) is connected with the input end of the second pipeline (63), the valve is arranged on the output end of the second pipeline (63), and the bottom end of the cleaning pump (62) is installed on the top end of the bracket (64).

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