Skin cell culture plate

Abstract

The utility model discloses a skin cell culture plate. The skin cell culture plate comprises a base plate, a seat plate and culture units, the seat plate is detachably connected to the base plate, and a liquid flowing channel is formed in a panel of the seat plate; the culture units are fixed on the top surface of the base plate; the culture unit comprises a liquid storage tank and a cell inoculation cup, and a culture medium solution inlet / outlet communicated with the liquid circulation channel is formed in the bottom wall of the liquid storage tank; two ends of the cell inoculation cup are communicated, and a porous membrane for inoculating cells is fixed at the bottom of the cell inoculation cup and can be propped against the liquid level of the liquid flowing channel. According to the cell inoculation cup, cells are inoculated on the porous membrane in the cell inoculation cup, a culture medium can enter the liquid circulation channel through the culture medium solution inlet and outlet, and the culture medium in the liquid circulation channel can provide nutrients for the cells on the porous membrane through the porous membrane, so that a gas-liquid interface model of the skin is constructed; the method can be used for research work in the research and development fields of cosmetics, medicines and the like.

Description

Technical Field

[0001] This utility model relates to the field of biomedical engineering technology, and more specifically to a skin cell culture plate. Background Technology

[0002] Biomedical tissue engineering is a discipline that combines cell culture and materials to reconstruct tissues or organs in vitro, typically using cell culture plates for cell culture.

[0003] Cell culture plates are a fundamental technology in tissue engineering and stem cell research, providing an effective tool for exploring cell behavior and studying physiological and pathological processes. They play a crucial role in these fields, allowing us to control and observe cell behavior, growth, and differentiation, thus enabling in-depth research into the principles and mechanisms of tissue engineering and stem cell applications.

[0004] In existing cell culture plates, external means are used to provide a certain rotation speed and eccentricity to oscillate the culture medium in the deep wells, increase the contact area between the culture medium and air, and improve liquid mixing to supply oxygen. However, the gas-liquid phase surface is small during the culture process, the liquid mixing effect is poor, and the cost is high and the operation is cumbersome.

[0005] Therefore, how to provide a skin cell culture plate that can effectively construct a gas-liquid interface skin model, is low in cost, and is easy to operate is a problem that urgently needs to be solved by those skilled in the art. Utility Model Content

[0006] In view of this, the present invention provides a skin cell culture plate, which aims to solve the above-mentioned technical problems.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] A skin cell culture plate includes a substrate, a base plate, and a culture unit;

[0009] The base plate is detachably connected to the base plate, and a liquid flow channel is provided on the panel of the base plate; the culture unit is fixed to the top surface of the base plate.

[0010] The culture unit includes a storage tank and a cell inoculation cup. The bottom wall of the storage tank has a culture medium solution inlet and outlet that communicates with the liquid flow channel. The cell inoculation cup is open at both ends and has a porous membrane fixed at its bottom for inoculating cells. The porous membrane can contact the liquid surface of the liquid flow channel.

[0011] The beneficial effects of this utility model are that the culture medium is added to the storage tank, and cells are inoculated on the porous membrane in the cell inoculation cup. The culture medium can enter the liquid flow channel through the culture medium solution inlet and outlet. The culture medium in the liquid flow channel can provide nutrients to the cells on the porous membrane through the porous membrane, thereby constructing a skin gas-liquid interface model, which can be used in research work in the fields of cosmetics, pharmaceuticals and other research and development.

[0012] Preferably, there are two liquid storage pools, which are symmetrical on both sides of the cell inoculation cup.

[0013] Preferably, there are multiple base plates, and the top surface of the substrate has multiple receiving grooves. The multiple base plates are embedded in the multiple receiving grooves in a one-to-one correspondence. Each base plate is provided with at least three culture units.

[0014] Preferably, the seat plate is a rectangular plate with a length of 75mm, a width of 25mm, and a height of 5-20mm.

[0015] Preferably, the diameter of the cell inoculation cup is 1-20 mm, the pore size of the porous membrane is 1-10 μm, and the volume of the storage tank is 0.1-5 ml.

[0016] Preferably, the substrate, the base plate, and the culture unit are all formed by injection molding of polymer materials.

[0017] Preferably, the liquid storage tank, cell inoculation cup, porous membrane and the seat plate are fixed together by adhesive bonding, thermoforming or laser welding.

[0018] Preferably, the height of the liquid flow channel is 0.2 to 1.5 mm.

[0019] Preferably, the system further includes a cover plate, which covers the top surface of the substrate and engages with the culture unit. The cover plate ensures the airtightness of the cell culture environment and prevents cell contamination.

[0020] As can be seen from the above technical solution, compared with the prior art, the present invention discloses a skin cell culture plate that can construct a skin model with an air-liquid interface. It is low in cost, simple to operate, and the substrate structure is compatible with existing cell culture plates, thus it is compatible with most existing detection equipment and user-friendly. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0022] Figure 1 This is a top view of the cross section of the culture plate provided by this utility model;

[0023] Figure 2 This is a side sectional view of the culture unit provided by this utility model.

[0024] in,

[0025] 1-Substrate; 2-Seat plate; 3-Cultivation unit; 4-Reservoir; 5-Cell inoculation cup; 6-Culture solution inlet / outlet; 7-Porous membrane; 8-Liquid flow channel. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0027] This utility model embodiment discloses a skin cell culture plate, including a substrate 1, a base plate 2, and a culture unit 3;

[0028] The base plate 2 is detachably connected to the base plate 1, and a liquid flow channel 8 is provided on the panel of the base plate 2; the culture unit 3 is fixed on the top surface of the base plate 2.

[0029] The culture unit 3 includes a storage tank 4 and a cell inoculation cup 5. The bottom wall of the storage tank 4 has a culture medium solution inlet / outlet 6 that communicates with the liquid flow channel 8. The cell inoculation cup 5 is open at both ends and has a porous membrane 7 fixed at its bottom for inoculating cells. The porous membrane 7 can contact the liquid surface of the liquid flow channel 8.

[0030] like Figure 2 As shown, in skin cell culture, cells are seeded on the top surface of a porous membrane in a cell seeding cup, and culture medium is added to a reservoir. The culture medium enters the liquid flow channel from the solution inlet / outlet on the bottom wall of the reservoir. The culture medium in the liquid flow channel permeates upward from the bottom surface of the porous membrane to provide nutrients to the cells, thereby constructing the air-liquid interface of the skin.

[0031] It should be noted that the skin-air-liquid interface refers to a culture method in which one side of a skin cell is exposed to air while the other side is in contact with a liquid culture medium during in vitro culturing. This method can better simulate the physiological environment of the skin in vivo, promote the directed differentiation and proliferation of epidermal cells, and form a skin model with multi-layered structure and function.

[0032] In this embodiment, there are two reservoirs 4, symmetrically located on both sides of the cell inoculation cup 5. The two reservoirs provide uniform and comprehensive nutrients to the cells, ensuring the effectiveness of cell culture.

[0033] To further optimize the above technical solution, there are multiple base plates 2, and multiple receiving slots are opened on the top surface of the substrate 1. The multiple base plates 2 are embedded in the multiple receiving slots in a one-to-one correspondence; each base plate 2 is provided with at least three culture units 3.

[0034] like Figure 1 As shown, the base plate 2 is a rectangular plate with a length of 75 mm, a width of 25 mm, and a height of 5-20 mm. There are three base plates, which are sequentially and parallelly embedded or inserted into the receiving groove of the base plate; the diameter of the cell inoculation cup 5 is 1-20 mm, the pore size of the porous membrane 7 is 1-10 μm, and the volume of the liquid storage tank 4 is 0.1-5 ml; the culture medium solution is supplied to the liquid flow channel through the two liquid storage tanks, and the culture medium in the liquid flow channel provides nutrients to the cells through the porous membrane.

[0035] In this embodiment, the liquid inlet and outlet of the culture medium and the porous membrane are on the same plane and both are located above the liquid flow channel. The porous membrane can contact the liquid surface in the liquid flow channel to ensure that the culture medium can permeate through the porous membrane to provide nutrients to the cells on the porous membrane.

[0036] To further optimize the above technical solution, substrate 1, base plate 2, and culture unit 3 are all formed by injection molding of polymer materials. Polymer materials include, but are not limited to, PS, PC, and PMMA.

[0037] To further optimize the above technical solution and ensure the integrity of the culture plate, the liquid storage tank 4, cell inoculation cup 5, porous membrane 7 and seat plate 2 are fixed together by adhesive bonding, thermoforming or laser welding.

[0038] To further optimize the above technical solution, the height of the liquid flow channel 8 is 0.2 to 1.5 mm.

[0039] To further optimize the above technical solution, a cover plate is also included, which is placed on the top surface of the substrate 1 and fastens the culture unit 3.

[0040] The cover plate improves the airtightness of the cell culture environment and prevents cell contamination. The cover plate can be snapped onto the periphery of the substrate or placed on the top surface of the substrate. To ensure the airtightness of the cover plate, a sealing gasket is provided between the lower end face of the cover plate and the periphery of the top surface of the substrate.

[0041] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on its differences from other embodiments. Similar or identical parts between embodiments can be referred to interchangeably. For the apparatus disclosed in the embodiments, since they correspond to the methods disclosed in the embodiments, the description is relatively simple; relevant parts can be referred to the method section.

[0042] The above description of the disclosed embodiments enables those skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A skin cell culture plate, characterized in that, It includes a substrate (1), a base plate (2), and a culture unit (3); The seat plate (2) is detachably connected to the base plate (1), and a liquid flow channel (8) is provided on the panel of the seat plate (2); the culture unit (3) is fixed on the top surface of the seat plate (2); The culture unit (3) includes a storage tank (4) and a cell inoculation cup (5). The bottom wall of the storage tank (4) is provided with a culture medium solution inlet and outlet (6) that communicates with the liquid flow channel (8). The cell inoculation cup (5) is open at both ends and has a porous membrane (7) fixed at its bottom for inoculating cells. The porous membrane (7) can contact the liquid surface of the liquid flow channel (8).

2. The skin cell culture plate according to claim 1, characterized in that, The number of the storage tanks (4) is two and they are symmetrical on both sides of the cell inoculation cup (5).

3. A skin cell culture plate according to claim 2, characterized in that, The number of the seat plates (2) is multiple, and the top surface of the substrate (1) is provided with multiple receiving grooves. The multiple seat plates (2) are embedded in the multiple receiving grooves in a one-to-one correspondence. Each seat plate (2) is provided with at least three culture units (3).

4. A skin cell culture plate according to claim 3, characterized in that, The seat plate (2) is a rectangular plate with a length of 75mm, a width of 25mm, and a height of 5-20mm.

5. A skin cell culture plate according to claim 1, characterized in that, The cell inoculation cup (5) has a diameter of 1-20 mm, the porous membrane (7) has a pore size of 1-10 μm, and the reservoir (4) has a volume of 0.1-5 ml.

6. A skin cell culture plate according to claim 1, characterized in that, The substrate (1), the base plate (2), and the culture unit (3) are all formed by injection molding of polymer materials.

7. A skin cell culture plate according to claim 6, characterized in that, The liquid storage tank (4), cell inoculation cup (5), porous membrane (7) and the seat plate (2) are fixed together by adhesive bonding, hot pressing or laser welding.

8. A skin cell culture plate according to claim 1, characterized in that, The height of the liquid flow channel (8) is 0.2 to 1.5 mm.

9. A skin cell culture plate according to any one of claims 1 to 8, characterized in that, It also includes a cover plate, which covers the top surface of the substrate (1) and fastens the culture unit (3).

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