Culture dish capable of fixing Wharton's jelly
By introducing a biocompatible coating and a sieve connected to a movable scaffold into Wharton's gel culture dishes, the problems of uneven Wharton's gel culture and cumbersome operation in existing technologies have been solved, achieving precise cell separation and efficient culture.
Patent Information
- Application Number
- CN202421992503.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-16
- Publication Date
- 2026-01-16
- Estimated Expiration
- 2034-08-16
AI Technical Summary
In existing Wharton's gel culture dishes, tissue blocks are of uneven size and suspended on the surface of the liquid during the peeling and culture process, making it difficult for umbilical cord mesenchymal stem cells to stably climb out. The operation is cumbersome, and the existing technology requires centrifugation and sieve filtration, which affects the culture efficiency and quality.
A culture dish for fixing Wharton's colloid was designed, employing a sieve structure with a biocompatible coating and a movable support, with a pore size of 100 micrometers, which facilitates screening and separation operations, reduces cell damage, and improves culture efficiency.
Through biocompatible coatings and active scaffold design, a cell-friendly growth environment is provided, enabling precise isolation of specific cells and efficient handling of cultures, significantly improving the quality and efficiency of cell culture.
Smart Images
Figure CN223805084U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the field of culture dish especially, a kind of culture dish of fixed Wharton's jelly. BACKGROUND
[0002] Umbilical cord stem cells are a kind of mesenchymal stem cells that can self-renew, highly proliferate and differentiate, which can be rapidly expanded in vitro culture, and have paracrine and immunoregulatory functions. Therefore, it is widely used in tissue repair, immunosuppression and medical beauty. Currently, the method for preparing umbilical cord mesenchymal stem cells is usually to peel the Wharton's jelly of the umbilical cord, and to carry out primary culture by tissue adhesion method. This method is to sterilize the umbilical cord with 75% alcohol, remove the arteries and veins, use a toothed forceps to peel the Wharton's jelly on the amnion, and then use tissue scissors to cut the Wharton's jelly into small pieces with a size of about 2mm 3 . Then, these small pieces are inoculated into culture bottles or culture dishes for primary culture.
[0003] In the existing culture dish of Wharton's jelly, the peeled Wharton's jelly is usually cut into small pieces with tissue scissors, resulting in uneven size. After adding complete culture medium into the bottle, many tissue pieces will usually be suspended in the liquid surface, and it is difficult for the tissue to adhere. At the same time, each time of microscopic observation and medium change will make the tissue pieces shake, and it is difficult for the umbilical cord mesenchymal stem cells to stably crawl out, resulting in a long period of primary culture. In addition, in the existing technology, centrifugation to remove supernatant is required in the full-volume medium change step, and it is easy to suck the tissue pieces in the half-volume medium change step. In the P0 to P1 cell harvesting stage, a 100μm sieve is also required to filter out the tissue pieces. Therefore, the technical personnel in the field provide a culture dish for fixing Wharton's jelly to solve the problems in the background art. SUMMARY
[0004] The utility model aims at solving the shortcomings in the prior art, and provides a culture dish for fixing Wharton's jelly. The frame, movable groove, movable block, handle and clamping groove movable support are connected through the clamping block and the sieve, the movable and detachable sieve design is convenient for screening and separating the culture, increases the operability and flexibility of the experiment, the pore size of the sieve is set to 100 microns, which can accurately screen out cells, particles or other substances of a specific size, provides accurate separation effect for the experiment or processing process, meets the needs of specific experiments or applications, the biocompatible coating is made of collagen, which provides a favorable environment for cell adhesion and growth, reduces cell damage and adverse reactions, and significantly improves the effect and quality of cell culture.
[0005] To achieve the above object, the utility model provides the following technical scheme: A culture dish capable of fixing Wharton's jelly, including culture dish body, the inside wall of culture dish body is equipped with biocompatibility coating, the centre of both side walls of culture dish body is equipped with movable support, one side wall of two movable supports is equipped with clamping block, two clamping blocks are fixedly connected with screen mesh;
[0006] Through the above technical scheme, the movable and detachable screen mesh is designed by the frame, the movable groove, the movable block, the handle and the clamping groove, and the screen mesh is connected by the clamping block, which facilitates the screening and separation operation of the culture, increases the operability and flexibility of the experiment, the pore size of the screen mesh is set to 100 microns, which can accurately screen the cells, particles or other substances of a specific size, provides accurate separation effect for the experiment or processing process, meets the needs of specific experiments or applications, and the biocompatibility coating is used, and the coating material is collagen, which provides a favorable environment for cell adhesion and growth, reduces cell damage and adverse reactions, and significantly improves the effect and quality of cell culture.
[0007] Further, the two movable supports include two frames, two movable grooves, two movable blocks, two handles and two clamping grooves, the two frames are arranged on the two side walls of the culture dish body, the two movable grooves are arranged at the center of the upper end faces of the two frames, the two movable blocks are arranged at the center of the interiors of the two movable grooves, the two handles are arranged at the center of the upper end faces of the two movable blocks, and the two clamping grooves are arranged at the upper center of one side wall of the two movable blocks.
[0008] Through the above technical scheme, the two frames are fixed on the two side walls of the culture dish body, the movable groove provides a moving track for the movable block, the movable block is located in the movable groove and can slide along the movable groove, the handle located at the center of the upper end face of the movable block can be used to apply an external force to move the movable block, the clamping groove is located at the upper center of one side wall of the movable block and is used for connecting and fixing the clamping block, which facilitates the taking and placing of the screen mesh and facilitates the screening and separation operation of the culture, thereby increasing the operability and flexibility of the experiment.
[0009] Further, the two clamping grooves are adapted to the two clamping blocks respectively.
[0010] Through the above technical scheme, the stability of the connection between the clamping block and the clamping groove is ensured, the screen mesh is prevented from loosening or falling off during use, and the smooth progress of the experiment or operation is ensured.
[0011] Further, recesses are formed at the upper center of one side wall of the two frames.
[0012] Through the technical scheme, the recess provides a containing space for the operation movable block, so that the movable block is prevented from being blocked by the frame during movement.
[0013] Further, the two movable blocks are respectively slidably connected inside the two movable grooves.
[0014] Through the technical scheme, the sieve screen can be positioned at a desired position in the culture dish, improving the accuracy and repeatability of experiments.
[0015] Further, the biocompatible coating material is collagen.
[0016] Through the technical scheme, collagen has good biocompatibility, can interact with cells and tissues, promote cell adhesion and growth, provide an environment conducive to cell survival and proliferation, reduce cell damage and adverse reactions, and improve the effect and quality of cell culture.
[0017] Further, the sieve screen has a pore size of 100 microns.
[0018] Through the technical scheme, specific size cells, particles or other substances can be effectively screened, providing accurate separation effect for experimental or processing process, meeting the needs of specific experiments or applications.
[0019] The utility model has the following beneficial effects:
[0020] 1、The utility model discloses a culture dish capable of fixing the Houghton's glue, which comprises a frame, a movable groove, a movable block, a handle and a clamping groove, and a sieve screen is connected to the movable block through a clamping block.
[0021] 2、The utility model discloses a culture dish capable of fixing the Houghton's glue, which comprises a frame, a movable groove, a movable block, a handle and a clamping groove, and a sieve screen is connected to the movable block through a clamping block.
[0022] 3、The utility model discloses a culture dish capable of fixing the Houghton's glue, which comprises a frame, a movable groove, a movable block, a handle and a clamping groove, and a sieve screen is connected to the movable block through a clamping block. DRAWINGS
[0023] Figure 1 A perspective view of the culture dish capable of fixing the Houghton's glue is provided.
[0024] Figure 2 A perspective view of the culture dish capable of fixing the Houghton's glue is provided.
[0025] Figure 3 A three-dimensional exploded view of the movable support of the culture dish capable of fixing Wharton's jelly is provided in the utility model;
[0026] Figure 4 For Figure 2 The enlarged schematic view at A in the middle.
[0027] Legend:
[0028] 1, culture dish body; 2, biocompatible coating; 3, movable support; 301, frame; 302, movable groove; 303, movable block; 304, handle; 305, clamping groove; 4, clamping block; 5, screening mesh. DETAILED DESCRIPTION
[0029] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.
[0030] With reference to Figures 1-4 The utility model provides an embodiment: a culture dish capable of fixing Wharton's jelly, including culture dish body 1, culture dish body 1 inner side wall is equipped with biocompatible coating 2, culture dish body 1 both side wall center is equipped with movable support 3, two movable supports 3 one side wall is equipped with clamping block 4, two clamping blocks 4 between fixed connection has screening mesh 5, culture dish body 1 is used to accommodate Wharton's jelly, and the biocompatible coating 2 of inner side wall provides good growth environment for Wharton's jelly, promotes its stable and healthy development, and the movable support 3 is arranged at the center of the both side walls of the culture dish body 1, the screening mesh 5 is fixed through the clamping block 4, the screening mesh 5 can be adjusted in position according to the experimental demand with the help of the movable support 3, so as to carry out screening or separation operation on Wharton's jelly in the culture dish, the biocompatible coating 2 is made of collagen, the collagen has good biocompatibility, can interact with cells and tissues, promotes the adhesion and growth of cells, provides an environment conducive to cell survival and proliferation, reduces the damage and adverse reactions of cells, improves the effect and quality of cell culture, the aperture of the screening mesh 5 is 100 microns, effectively screens out cells, particles or other substances of specific size, provides accurate separation effect for the experimental or processing process, meets the demand of specific experiment or application.
[0031] Two movable supports 3 comprise two frames 301, two movable grooves 302, two movable blocks 303, two handles 304 and two clamping grooves 305, the two frames 301 are arranged on the two side walls of the petri dish body 1, the two movable grooves 302 are arranged at the center of the upper end faces of the two frames 301 respectively, the two movable blocks 303 are arranged at the center of the interiors of the two movable grooves 302 respectively, the two handles 304 are arranged at the center of the upper end faces of the two movable blocks 303 respectively, the two clamping grooves 305 are arranged at the upper center of one side wall of the two movable blocks 303 respectively, the two clamping blocks 4 are arranged in the interiors of the two clamping grooves 305 respectively, the two frames 301 are fixed on the two side walls of the petri dish body 1, the movable groove 302 provides a moving track for the movable block 303, the movable block 303 is located in the interior of the movable groove 302 and can slide along it, through the handle 304 located at the center of the upper end face of the movable block 303, an external force can be applied to move the movable block 303, the clamping groove 305 is located at the upper center of one side wall of the movable block 303 and is used for connecting and fixing the clamping block 4, which is convenient for taking out and placing the sieve screen 5, is convenient for screening and separating the culture, increases the operability and flexibility of the experiment, the two clamping grooves 305 and the two clamping blocks 4 are mutually adapted, which ensures the stability of the connection between the clamping block 4 and the clamping groove 305 and prevents the sieve screen 5 from loosening or falling off during use, ensuring the smooth progress of the experiment or operation, recesses are arranged at the upper center of one side wall of the two frames 301, the recesses provide a containing space for the operation of the movable block 303, avoiding that the movable block 303 is blocked during movement, the two movable blocks 303 are slidably connected in the interiors of the two movable grooves 302 respectively, so that the sieve screen 5 can be located at the required position in the petri dish, improving the accuracy and repeatability of the experiment.
[0032] Working principle: The petri dish body 1 is used for containing the Wharton's jelly, the biocompatible coating 2 of the inner side wall provides a good growth environment for the Wharton's jelly, promotes its stable and healthy development, the movable support 3 is arranged at the center of the two side walls of the petri dish body 1, the sieve screen 5 is fixed by the clamping block 4, the sieve screen 5 can be used according to the experimental requirements, the two frames 301 are fixed on the two side walls of the petri dish body 1, the movable groove 302 provides a moving track for the movable block 303, the movable block 303 is located in the interior of the movable groove 302 and can slide along it, through the handle 304 located at the center of the upper end face of the movable block 303, an external force can be applied to move the movable block 303, the clamping groove 305 is located at the upper center of one side wall of the movable block 303 and is used for connecting and fixing the clamping block 4, which is convenient for taking out and placing the sieve screen 5, is convenient for screening and separating the culture, increases the operability and flexibility of the experiment, thereby the Wharton's jelly in the petri dish is screened or separated.
[0033] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and is not intended to limit the present application, although the foregoing embodiments of the present application has been described in detail, for the skilled in the art, it still can be modified, or for part of the technical features of the equivalent replacement, the spirit and principles of the present application, made any modification, equivalent replacement, improvement, etc., should be included within the scope of the present application.
Claims
1. A petri dish which can immobilize Wharton's jelly comprising a petri dish body (1), characterized in that: The inside wall of the culture dish body (1) is sleeved with a biocompatible coating (2), the center of the two side walls of the culture dish body (1) is provided with a movable support (3), one side wall of the two movable supports (3) is provided with a clamping block (4), and the two clamping blocks (4) are fixedly connected with a screening net (5).
2. A culture dish to which a Wharton's jelly can be fixed according to claim 1, characterized in that: The two movable supports (3) comprise two frames (301), two movable grooves (302), two movable blocks (303), two handles (304) and two clamping grooves (305), the two frames (301) are arranged on the two side walls of the culture dish body (1), the two movable grooves (302) are arranged at the center of the upper end faces of the two frames (301), the two movable blocks (303) are arranged at the centers of the interiors of the two movable grooves (302), the two handles (304) are arranged at the centers of the upper end faces of the two movable blocks (303), and the two clamping grooves (305) are arranged at the upper centers of the side walls of the two movable blocks (303).
3. A culture dish to which a Wharton's jelly can be fixed according to claim 2, characterized in that: The two clamping grooves (305) and the two clamping blocks (4) are matched with each other.
4. A Wharton's jelly immobilizable petri dish according to claim 2, wherein: The upper center of the side wall of the two frames (301) is provided with a groove.
5. A Wharton's jelly immobilizable petri dish according to claim 2, wherein: The two movable blocks (303) are slidably connected in the interiors of the two movable grooves (302).
6. The Wharton's Jelly anchoring culture dish of claim 1, wherein: The biocompatible coating (2) is made of collagen.
7. The Wharton's Jelly anchoring culture dish according to claim 1, wherein: The aperture of the screening net (5) is 100 microns.