Culture device for mouse oocytes
By designing a device that includes an incubator, a guiding component, and a heating and humidification system, the problem of heat stress caused by temperature differences in oocyte culture was solved, achieving seamless replacement of nutrient solution and environmental stability, thus ensuring the physiological stability of cells.
Patent Information
- Application Number
- CN202520211609.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-11
- Publication Date
- 2026-01-16
- Estimated Expiration
- 2035-02-11
AI Technical Summary
During oocyte culture, the temperature difference between the new nutrient solution and the old solution causes a heat stress response in the cells. Current technology makes it difficult to achieve a seamless transition, which affects the physiological stability of the cells.
A device was designed that includes an incubator, a top cover, a nutrient tray, a guide assembly, a heating box, and a humidification box. The cell culture vessel is moved horizontally by an electric motor to achieve seamless replacement of the nutrient solution. The heating box maintains a constant temperature, the humidification box maintains humidity, and a rubber strip blocks the liquid outlet to ensure the stability of temperature and humidity.
It enables seamless replacement of nutrient solution, avoids cell heat stress response, maintains stable temperature and humidity in the culture environment, and ensures the physiological stability of cells.
Smart Images
Figure CN223805102U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to biological culture device field, in particular to a kind of culture device for mouse oocyte. BACKGROUND
[0002] Oocyte is the largest cell in human body, and it is wrapped by a layer of "eggshell" structure composed of glycoprotein, therefore, oocyte is suspended in culture medium during in vitro culture.
[0003] During oocyte culture, the nutrient solution needs to be replaced, but it is difficult to achieve seamless connection of the temperature of new solution and old solution. The new nutrient solution is often placed in the external environment, and there is a temperature difference between the new nutrient solution and the original culture solution in which the cells are located. When the new nutrient solution is added to the culture dish, the instantaneous temperature change will cause the oocyte to suffer heat stress, inducing a series of physiological stress responses in the cells.
[0004] Therefore, a culture device for mouse oocyte is provided. SUMMARY
[0005] To solve the problems raised in the background art, the utility model provides a culture device for mouse oocyte.
[0006] The technical solution of the utility model is as follows:
[0007] A culture device for mouse oocyte comprises a culture box and a top cover. The top cover is connected to the top surface of the culture box. The inside of the culture box is fixedly connected with symmetrically arranged first and second nutrient discs. The bottom surface of the first nutrient disc is tightly attached to a cell vessel. The bottom surface of the second nutrient disc is provided with a baffle along the direction from the waste liquid box to the second nutrient disc. The inner wall bottom surface of the second nutrient disc is provided with equidistantly distributed liquid guide holes. The inner wall of the culture box is provided with a guide assembly. The cell vessel is replaced by the baffle through the guide assembly. The inside of the top cover is provided with a heating box. The two sides of the heating box are provided with humidifying boxes. The inside of each humidifying box is attached with a sterile humidifying sheet. The heating box and the humidifying boxes are in communication with the culture box.
[0008] In a further technical solution, the guide assembly comprises a motor fixed to one end of the culture box. The output end of the motor is fixedly connected with a lead screw located on one side of the inside of the culture box. The lead screw is connected to the inner wall of the culture box through a bearing seat. The outer surface of the lead screw is threadedly connected with a sliding sleeve. One side wall of the sliding sleeve is fixedly connected with the side wall of the cell vessel. The inner wall of the culture box is fixedly connected with a guide strip. The other side wall of the sliding sleeve is in sliding cooperation with the guide strip. The end of the culture box away from the motor is provided with a sealing port. The inner wall of the sealing port is provided with a sealing strip. One side of the sealing strip is hingedly connected to the culture box.
[0009] Through the technical scheme, the sliding sleeve can be driven by the motor to be threadedly driven, so that the cell container is horizontally moved to below the second nutrient tray, the baffle is pushed to the opening of the sealing opening in the process, the sealing strip is ejected, and after the baffle is completely located below the second nutrient tray, the baffle can be pulled out by the worker to close the sealing opening.
[0010] In a further technical scheme, the cell container is provided below the waste liquid box fixed to the inner wall of the incubator, and the bottom surface of the waste liquid box is communicated with the outside through the connecting pipe.
[0011] Through the technical scheme, the connecting pipe is sleeved with a sealing ring, the temperature of the whole device is not lost, and when the cell container is moved, the waste liquid in the first nutrient tray flows into the waste liquid box, a part of the waste liquid is retained on the cell container covering the cells, and the device can facilitate the treatment of the waste liquid.
[0012] In a further technical scheme, the top surface of the heating box is fixedly connected with equidistantly distributed fans, the outlet of the fan is provided below a constant-temperature heating rod fixed in the heating box, the bottom end of the heating box is below the bottom surface of the top cover, and the bottom of the heating box is provided with through holes on both sides.
[0013] Through the technical scheme, hot air can be blown out of the through hole and into the incubator.
[0014] In a further technical scheme, the top surface of the top cover is detachably screwed, the bottom surface of the humidifying box is fixedly connected with a mesh plate, and the sterile humidifying sheet is located on the surface of the mesh plate.
[0015] Through the technical scheme, the humidity in the incubator can be maintained, and after the top cover of the incubator is detached, the sterile humidifying sheet can be replaced to maintain the humidity.
[0016] In a further technical scheme, the bottom surface of the second nutrient tray is symmetrically provided with a through-type sliding groove, and the top surface of the baffle is symmetrically provided with a rubber strip.
[0017] Through the technical scheme, the baffle can be pushed out of the bottom surface of the second nutrient tray, and the rubber strip can be arranged to enable the baffle to be located at the bottom surface of the second nutrient tray to block the liquid guide opening without being pushed.
[0018] Compared with the prior art, the utility model has the following beneficial effects:
[0019] The device can directly drive the guiding assembly to move the cell container horizontally close to the bottom surface of the second nutrient tray when the waste liquid needs to be replaced, and the nutrient liquid in the second nutrient tray can be retained in the second nutrient tray when the baffle is not pushed out, and the baffle can be pushed out to the end of the incubator with the sealing opening along with the cell container continuously close to the baffle, until the cell container replaces the position of the baffle, the liquid guiding hole can be completely communicated with the cell container, so that the new nutrient liquid leaks into the cell container, and the optimization of the device can directly replace the waste liquid with the same temperature nutrient liquid. BRIEF DESCRIPTION OF DRAWINGS
[0020] Figure 1 is the overall structure schematic diagram of the utility model;
[0021] Figure 2 is the internal structure schematic diagram of the incubator and the top cover of the utility model;
[0022] Figure 3 is the partial section structure schematic diagram of the incubator of the utility model;
[0023] Figure 4 is the overhead structure schematic diagram of the incubator of the utility model;
[0024] Figure 5 is the structure schematic diagram of the humidifying box of the utility model;
[0025] Figure 6 is the structure schematic diagram of the heating box of the utility model;
[0026] Figure 7 is the assembly structure schematic diagram of the second nutrient tray and the baffle of the utility model.
[0027] BRIEF DESCRIPTION OF DRAWINGS:
[0028] 1, incubator;2, top cover;3, first nutrient tray;4, second nutrient tray;5, cell container;6, liquid guiding hole;7, baffle;8, heating box;9, constant temperature heating rod;10, through hole;11, fan;12, humidifying box;13, sterile humidifying sheet;14, screen;19, sealing opening;20, sealing strip;21, waste liquid box;
[0029] Guiding assembly: 15, motor;16, screw;17, sliding sleeve;18, guiding strip. DETAILED DESCRIPTION
[0030] In order to make the technical means, creative characteristics, purposes and effects of the utility model easy to understand, the utility model is further described below in combination with specific embodiments.
[0031] In the description of the utility model, it needs to explain, the term "upper", "lower", "internal", "external", "front end", "rear end", "both ends", "one end", "the other end" and so on indicate the position relation or position relation based on the position relation shown in the drawing, only for the convenience of describing the utility model and simplifying the description, and not indicate or imply that the device or element indicated must have a particular orientation, a particular orientation and operation, therefore, it cannot be understood as a limitation on the utility model. In addition, the terms "first", "second" are only for the purpose of description, and cannot be understood as indicating or implying relative importance.
[0032] In the description of the utility model, it needs to explain, unless otherwise expressly provided and limited, the terms "mounting", "provided with", "connection" and the like should be broadly understood, for example, "connection", can be fixed connection, can also be detachable connection, or integrally connected, can be mechanical connection, can also be electrical connection, can be directly connected, can also be indirectly connected through an intermediate medium, can be the communication inside two elements. For ordinary skilled in the art, the specific meaning of the above terms in the utility model can be understood according to the specific circumstances.
[0033] Embodiment:
[0034] Please refer to Figures 1-6 A culture device for mouse oocytes, comprising a culture box 1, a top cover 2, the top cover 2 is connected with the top surface of the culture box 1, the inside of the culture box 1 is fixedly connected with symmetrically arranged first nutrient dish 3, second nutrient dish 4, the bottom surface of the first nutrient dish 3 is closely attached with cell vessels 5, the bottom surface of the second nutrient dish 4 is provided with a baffle 7 along the direction of the waste liquid box 21 to the second nutrient dish 4, the inner wall bottom surface of the second nutrient dish 4 is provided with equidistantly distributed liquid guide openings 6, the inner wall of the culture box 1 is provided with a guide assembly, the cell vessels 5 are replaced by the baffle 7 through the guide assembly, the inside of the top cover 2 is provided with a heating box 8, both sides of the heating box 8 are provided with humidifying boxes 12, the inside of the humidifying box 12 is attached with sterile humidifying sheets 13, and the heating box 8 and the humidifying box 12 are in communication with the culture box 1.
[0035] Please refer to Figure 4 The guide assembly comprises a motor 15 fixed at one end of the culture box 1, the output end of the motor 15 is fixedly connected with a lead screw 16 located at one side in the culture box 1, and the lead screw 16 is connected with the inner wall of the culture box 1 through a bearing seat, the outer surface of the lead screw 16 is threadedly connected with a sliding sleeve 17, one side wall of the sliding sleeve 17 is fixedly connected with the side wall of the cell vessels 5, the inner wall of the culture box 1 is fixedly connected with a guide strip 18, the other side wall of the sliding sleeve 17 is in sliding fit with the guide strip 18, one end of the culture box 1 away from the motor 15 is provided with a sealing opening 19, the inner wall of the sealing opening 19 is provided with a sealing strip 20, and one side of the sealing strip 20 is hinged with the culture box 1.
[0036] By driving the motor 15, the sliding sleeve 17 can be screwed, and then the cell container 5 is horizontally moved to the lower side of the second nutrient plate 4, and the baffle 7 is pushed to the opening of the sealing port 19, and the sealing strip 20 is pushed out, until the baffle 7 is completely located below the second nutrient plate 4, and then the baffle 7 can be pulled out by the staff, and the sealing port 19 is closed.
[0037] Please refer to Figure 2 and Figure 3 , the lower side of the cell container 5 is provided with a waste liquid box 21 fixed to the inner wall of the incubator 1, and the bottom surface of the waste liquid box 21 is communicated with the outside through a connecting pipe.
[0038] The connecting pipe part is sleeved with a sealing ring, so that the temperature of the whole device is not lost, and when the cell container 5 is moved, the waste liquid in the first nutrient plate 3 flows into the waste liquid box 21, and a part is reserved in the cell container 5 covering the cells. The arrangement of the device can facilitate the treatment of waste liquid.
[0039] Please refer to Figure 2 and Figure 6 The top surface of the heating box 8 is fixedly connected with equidistant distributed fans 11, and the outlet of the fan 11 is provided below the constant temperature heating rod 9 fixed in the heating box 8. The bottom end of the heating box 8 is located below the bottom surface of the top cover 2, and the bottom of the heating box 8 is provided with through holes 10 on both sides.
[0040] Conveniently blow hot air out of the through hole 10 into the incubator 1.
[0041] Please refer to Figure 1 and Figure 2 The top surface of the top cover 2 is screw disassembled, the bottom surface of the humidifying box 12 is fixedly connected with a mesh plate 14, and the sterile humidifying sheet 13 is located on the surface of the mesh plate 14.
[0042] Conveniently maintain the humidity in the incubator 1, and after the top cover of the incubator 1 is disassembled, the sterile humidifying sheet 13 can be replaced to maintain the humidity.
[0043] Please refer to Figure 7 The bottom surface of the second nutrient plate 4 is symmetrically provided with a through type sliding groove, and the top surface of the baffle 7 is symmetrically provided with a rubber strip.
[0044] Conveniently push the baffle 7 out of the bottom surface of the second nutrient plate 4, and the rubber strip can make the baffle 7 block the liquid guide port 6 on the bottom surface of the second nutrient plate 4 without pushing force.
[0045] In operation, the mouse oocyte is placed in the cell vessel 5 which is tightly attached to the bottom of the first nutrient plate 3. During the cell culture, the constant heating rod 9 in the heating box 8 continuously generates heat, and the fan 11 blows the hot air out of the through hole 10 into the incubator 1 to maintain the appropriate temperature in the incubator 1. The sterile humidifying sheet 13 in the humidifying box 12 slowly releases water vapor through the mesh plate 14 to ensure the stable humidity in the incubator 1. When the nutrient solution needs to be replaced, the motor 15 is started to rotate the lead screw 16, and the sliding sleeve 17 connected with the lead screw 16 starts to move horizontally with the assistance of the guide strip 18. Since the sliding sleeve 17 is fixedly connected with the side wall of the cell vessel 5, the cell vessel 5 moves horizontally to the bottom of the second nutrient plate 4. The cell vessel 5 continuously approaches the baffle 7 to apply a pushing force to the baffle 7, so that the baffle 7 slides along the symmetrical through-type sliding groove arranged on the bottom of the second nutrient plate 4 to the end of the incubator 1 with the sealing port 19, until the ejection sealing strip 20 is ejected. When the cell vessel 5 completely replaces the position of the baffle 7, the liquid guide port 6 is communicated with the cell vessel 5. During the movement of the cell vessel 5, the waste liquid in the first nutrient plate 3 flows into the waste liquid box 21 below, but a part of the waste liquid remains in the cell vessel 5 to cover the cells and avoid the damage of the cells due to dryness.
[0046] The above-described embodiments only express the specific implementation manners of the present application, and the description is relatively specific and detailed, but it should not be understood as the limitation of the patent scope of the present application. It should be noted that, for ordinary skilled in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which all belong to the protection scope of the present application.
Claims
1. A culture device for mouse oocytes, comprising a culture box (1) and a top cover (2) which is connected to the top surface of the culture box (1), characterized in that, The inside of the incubator (1) is fixedly connected with symmetrically arranged first nutrient tray (3), second nutrient tray (4), the bottom surface of the first nutrient tray (3) is closely combined with cell vessel (5), the bottom surface of the second nutrient tray (4) is inserted with baffle (7) along the direction of waste liquid box (21) to the second nutrient tray (4), the inner wall bottom surface of the second nutrient tray (4) is provided with equidistantly distributed liquid guide hole (6), the inner wall of the incubator (1) is provided with guide assembly, the cell vessel (5) is replaced by the guide assembly baffle (7), the inside of the top cover (2) is provided with heating box (8), both sides of the heating box (8) are provided with humidification box (12), the inside of the humidification box (12) is combined with sterile humidification sheet (13), and the heating box (8) and the humidification box (12) are in communication with the incubator (1).
2. The culture device for mouse oocytes according to claim 1, wherein The guide assembly comprises a motor (15) fixed to one end of the incubator (1), the output end of the motor (15) is fixedly connected with a lead screw (16) located on one side of the inside of the incubator (1), and the lead screw (16) is connected with the inner wall of the incubator (1) through a bearing seat, the outer surface of the lead screw (16) is threadedly connected with a sliding sleeve (17), one side wall of the sliding sleeve (17) is fixedly connected with the side wall of the cell vessel (5), the inner wall of the incubator (1) is fixedly connected with a guide strip (18), the other side wall of the sliding sleeve (17) is in sliding fit with the guide strip (18), one end of the incubator (1) away from the motor (15) is provided with a sealing port (19), the inner wall of the sealing port (19) is inserted with a sealing strip (20), and one side of the sealing strip (20) is hinged to the incubator (1).
3. The culture device for mouse oocytes according to claim 1, wherein The lower side of the cell vessel (5) is provided with a waste liquid box (21) fixed to the inner wall of the incubator (1), and the bottom surface of the waste liquid box (21) is communicated with the outside through a connecting pipe.
4. The culture device for mouse oocytes according to claim 1, wherein The top surface of the heating box (8) is fixedly connected with equidistantly distributed fans (11), the outlet of the fan (11) is provided with a constant temperature heating rod (9) fixed in the inside of the heating box (8), the bottom end of the heating box (8) is located below the bottom surface of the top cover (2), and the bottom of the heating box (8) is provided with through holes (10) on both sides.
5. The culture device for mouse oocytes according to claim 1, wherein The top surface of the top cover (2) is screw dismountable, the bottom surface of the humidification box (12) is fixedly connected with a mesh plate (14), and the sterile humidification sheet (13) is located on the surface of the mesh plate (14).
6. The culture device for mouse oocytes according to claim 1, wherein The bottom surface of the second nutrient tray (4) is symmetrically provided with a through-type sliding groove, and the top surface of the baffle (7) is symmetrically provided with a rubber strip.