Blood smear dyeing device

Abstract

The utility model discloses a blood smear dyeing device. The blood smear staining device comprises a fixer for fixing a glass slide, a guide frame for guiding the moving direction of the fixer, a first fixing mechanism for mounting a push plate, a second fixing mechanism for mounting a staining agent box, a third fixing mechanism for mounting a buffer solution box, and a third fixing mechanism for mounting the first fixing mechanism, the second fixing mechanism and the third fixing mechanism comprise turntables, mounting columns for mounting the turntables, cleaners for cleaning and a waste liquid box for collecting waste liquid, the fixers are slidably arranged on the guide frame, and the turntables are rotatably arranged on the mounting columns; the turntable is rotated to adjust the first fixing mechanism, the second fixing mechanism or the third fixing mechanism to be aligned with the fixer, and the waste liquid box is positioned below the guide frame. The blood smear dyeing device can guarantee the quality of the blood smear and is convenient to dye.

Description

TECHNICAL FIELD

[0001] The utility model relates to blood smear dyeing technical field, in particular to a blood smear dyeing device. BACKGROUND

[0002] Blood cell morphology examination is a kind of examination method for diagnosing, differential diagnosis and disease monitoring by observing the morphology, size, structure and quantity characteristics of various blood cells in blood. Usually, blood smear is prepared and dyed, and then the morphology, size, quantity and distribution characteristics of red blood cells, white blood cells and platelets are observed under optical microscope.

[0003] Preparation of blood smear will place a drop of blood on a glass slide, and then use a smooth-edged push piece, the push piece is contacted with the glass slide at 30°-45° angle, after the push piece is contacted with blood, blood diffuses along the edge of the push piece, then the push piece is pushed steadily, and blood forms a uniform film on the glass slide. The thickness of the blood film formed by the push piece when pushing completely depends on the experience of the operator, too thick film will cause cell overlapping, and it is difficult to observe cell morphology clearly, and too thin film may make cell distribution too sparse, which is not conducive to finding abnormal cells. After the smear is completed, the natural drying is used, and then the dyeing agent is used for dyeing, after dyeing, 30s is waited, and then buffer is further dyed, and after 5min, water is used for washing. When preparing and dyeing, the dyeing agent is coated on the glass slide, so the dyeing agent will overflow, and the dyeing agent will be everywhere, and the environment is polluted. UTILITY MODEL CONTENT

[0004] The utility model solves the technical problem that a blood smear dyeing device capable of ensuring the thickness precision of blood film and collecting overflowed dyeing agent is provided.

[0005] To solve the above problems, the utility model provides a blood smear dyeing device, the blood smear dyeing device includes a fixer for fixing a glass slide, a guide frame for guiding the moving direction of the fixer, a first fixing mechanism for installing a push plate, a second fixing mechanism for installing a dyeing agent box, a third fixing mechanism for installing a buffer solution box, a rotating disc for installing the first fixing mechanism, the second fixing mechanism and the third fixing mechanism, a mounting column for installing the rotating disc, a cleaner for cleaning and a waste liquid box for collecting waste liquid, the fixer is slidably arranged on the guide frame, the rotating disc is rotatably arranged on the mounting column, the rotating disc is rotated to adjust the alignment of the first fixing mechanism, the second fixing mechanism or the third fixing mechanism with the fixer, and the waste liquid box is located below the guide frame.

[0006] Further, the fixer comprises a fixing seat for placing a slide, a clamping plate for clamping the slide on the fixing seat, a connecting rod for being connected with the clamping plate, a reset spring for resetting the clamping plate and applying a clamping force to the clamping plate, and a limiting plate for limiting the reset spring, the connecting rod is fixed through the fixing seat and the limiting plate, and two ends of the reset spring abut against the fixing seat and the limiting plate.

[0007] Further, the fixing seat is provided with a fixing groove for fixing the slide, and the depth of the fixing groove is less than the thickness of the slide.

[0008] Further, the guide frame comprises guide rails for guiding the fixing seat, and first and second vertical plates for mounting the guide rails, and the waste liquid box is located below the guide rails, and the first and second vertical plates are respectively located at two ends of the waste liquid box.

[0009] Further, the second vertical plate is located close to one end of the mounting column, and the top surface of the second vertical plate is lower than the groove bottom of the fixing groove.

[0010] Further, the second vertical plate is further provided with a limiting portion for limiting the fixing seat.

[0011] Further, two ends of the limiting plate protrude from the fixing seat.

[0012] Further, the first fixing mechanism comprises a mounting plate for placing a push plate, a fixing plate for fixing the push plate, and a connecting seat for connecting the mounting plate with the rotating disc, the mounting plate is hinged with the connecting seat through a hinge, the mounting plate is provided with a positioning notch, the bottom surface of the positioning notch is provided with a sponge pad, and the fixing plate is provided with an anti-skid pad for increasing friction.

[0013] Further, the positioning notch has a first positioning edge and a second positioning edge, the first positioning edge is used for limiting the alignment of the push plate and the slide, and the second positioning edge is used for limiting the spacing between the push plate and the slide.

[0014] Further, the mounting column is provided with a containing hole, the containing hole is provided with a positioning pin and a positioning spring, the positioning pin abuts against the spring, a sealing plug is screwed on the containing hole, part of the positioning pin is exposed from the sealing plug, the rotating disc is provided with a positioning sleeve, and the positioning sleeve is provided with a positioning hole matched with the positioning pin.

[0015] The blood smear staining device fixes the slide by using the fixer, fixes the push plate by using the first fixing mechanism, and fixes the spacing between the slide and the push plate, so that the thickness of the blood film is ensured, the waste liquid box is arranged below the guide frame, waste liquid is collected, and the environment is not polluted. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 is a preferred embodiment structure diagram of the blood smear staining device.

[0017] Figure 2 is a sectional view of the fixator.

[0018] Figure 3 is a structure diagram of the fixing seat.

[0019] Figure 4 is a structure diagram of the guide frame.

[0020] Figure 5 is a structure diagram of the first fixing mechanism.

[0021] Figure 6 is a structure diagram of the mounting plate after turning over.

[0022] Figure 7 is Figure 5 is a local enlarged view of A in the figure.

[0023] Figure 8 is a structure diagram of the mounting plate.

[0024] Figure 9 is a structure diagram of the positioning pin and the positioning hole.

[0025] The meanings of various signs in the drawings are as follows:

[0026] Guide frame 1, first vertical plate 11, second vertical plate 12, limiting part 121, guide sliding rail 13, fixator 2, fixing seat 21, fixing groove 211, clamping plate 22, return spring 23, connecting rod 24, limiting plate 25, waste liquid box 3, mounting column 41, rotating disc 42, cleaner 5, first fixing mechanism 6, fixing plate 61, mounting plate 62, positioning notch 621, first positioning edge 6211, second positioning edge 6212, connecting seat 63, anti-skid pad 64, sponge pad 65, second fixing mechanism 71, third fixing mechanism 72, glass slide 81, push plate 82, staining agent box 83, buffer solution box 84, positioning pin 91, positioning spring 92, sealing plug 93. DETAILED DESCRIPTION

[0027] The utility model will be further described in connection with the drawings.

[0028] As Figure 1The preferable embodiment of the blood smear staining device shown in the utility model includes a guide frame 1, a fixer 2, a waste liquid box 3, a mounting column 41, a rotating disc 42, a cleaner 5, a first fixing mechanism 6, a second fixing mechanism 71 and a third fixing mechanism 72. The fixer 2 is used for fixing a glass slide 81, the fixer 2 is slidingly arranged on the guide frame 1, and the guide frame 1 is used for guiding the fixer 2 to move along a predetermined direction. The first fixing mechanism 6 is used for mounting a push piece, the push piece is detachably fixed on the first fixing mechanism 6, the push piece is convenient to replace, and cross contamination is avoided. The second fixing mechanism 71 is used for fixing a staining agent box 83 storing a staining agent, the third fixing mechanism 72 is used for fixing a buffer liquid box 84 storing a buffer liquid, the first fixing mechanism 6, the second fixing mechanism 71 and the third fixing mechanism 72 are all fixed on the rotating disc 42, and the first fixing mechanism 6, the second fixing mechanism 71 and the third fixing mechanism 72 are distributed at intervals of 90° on the rotating disc 42. The rotating disc 42 is rotationally arranged on the mounting column 41, one side of the rotating disc 42 close to the guide frame 1 is an operation position, the mounting column 41 is fixed on the guide frame 1, of course, in other embodiments, the mounting column 41 can also not be fixed on the guide frame 1, but be fixed on other articles. The cleaner 5 is mounted on the waste liquid box 3, the cleaner 5 is used for connecting an external cleaning liquid source, the cleaning liquid sprayed by the cleaner 5 is used for cleaning the glass slide 81, and the excess staining agent is removed; the cleaner 5 adopts a rotatable faucet, the faucet can be rotated to one side of the waste liquid box 3 when not in use, the line of sight and operation are not affected, of course, in other embodiments, the cleaner 5 can also be provided with a valve pipe. The waste liquid box 3 is located below the guide frame 1, the waste liquid box 3 is used for collecting waste liquid, and contains the overflowed staining agent and the cleaned cleaning liquid. The waste liquid box 3 is provided with a drain, a pipe is arranged at the drain and connected with a sewage pipe of a department; it should be known that the drain can also not be arranged, and when the waste liquid box 3 accumulates to a certain degree, the waste liquid box 3 is taken out to pour the waste liquid into the sewage pipe. The rotating disc 42 can also be used for placing an ear cleaning ball, so that drying is accelerated.

[0029] First, the blood-dropped slide 81 is fixed on the fixing device 2, or the slide 81 is fixed and then blood is dropped, then the fixing device 2 is pushed to move on the guide frame 1 to the direction of the rotating disc 42, after moving to a certain distance, the rotating disc 42 is rotated to align the first fixing mechanism 6 provided with a push piece with the slide 81, that is, the push piece is located at the rear side of the blood, the fixing device 2 is moved to diffuse the blood along the edge of the push piece, after diffusion, the fixing device 2 is pushed to the direction away from the rotating disc 42 to form a blood film on the slide 81, after the blood film is dried, a blood smear is formed, then the rotating disc 42 is rotated to turn the second fixing mechanism 71 to the operating position, at the same time, the blood smear is pushed to a predetermined position, then the dyeing agent box 83 is squeezed to squeeze the dyeing agent on the blood smear, at the same time, the dyeing agent box 83 is slowly moved away from the rotating disc 42 to ensure that the dyeing agent is attached to the blood smear; after 30 seconds, the rotating disc 42 is rotated to turn the second fixing mechanism 71 to the operating position, and the buffer solution is squeezed and dropped on the blood smear; after 5 minutes, the cleaner 5 is opened to clean the blood smear, and the cleaning is completed.

[0030] As shown in Figure 2 and Figure 3 , the fixing device 2 comprises a fixing seat 21, a clamping plate 22, a reset spring 23, a connecting rod 24 and a limiting plate 25, the fixing seat 21 is slidably arranged on the guide frame 1, the clamping plate 22 is located above the fixing seat 21, and the clamping plate 22 is used for clamping the slide 81 on the fixing seat 21; in order to accurately fix the position of the slide 81, a fixing groove 211 is arranged on the fixing seat 21, the fixing groove 211 is arranged along the moving direction of the fixing seat 21, one end of the fixing groove 211 is communicated with the outside, that is, the end face of the one end of the fixing groove 211 is flush with the side wall of the fixing seat 21, and the depth of the fixing groove 211 is smaller than the thickness of the slide 81, so that the top surface of the slide 81 is higher than the top surface of the fixing seat 21, and the slide 81 is not interfered by the fixing seat 21 during operation. The connecting rod 24 is arranged on the clamping plate 22, the connecting rod 24 penetrates through the fixing seat 21 and is connected and fixed with the limiting plate 25, the reset spring 23 is sleeved on the connecting rod 24, the reset spring 23 abuts against the fixing seat 21 and the limiting plate 25, the reset spring 23 is used for resetting the clamping plate 22 and applying a pressing force to the clamping plate 22, so as to ensure that the clamping plate 22 can press the slide 81. The two ends of the limiting plate 25 protrude out of the fixing seat 21, so that the clamping plate 22 is pushed to move upward by pulling the limiting plate 25 upward, thereby facilitating the placement of the slide 81 on the fixing seat 21 or the removal of the dyed blood smear from the fixing seat 21.

[0031] As shown in Figure 4As shown, the guide frame 1 comprises a first vertical plate 11, a second vertical plate 12 and two guide rails 13, the two guide rails 13 are arranged side by side, and the two ends of each guide rail 13 are respectively mounted on the first vertical plate 11 and the second vertical plate 12, and the waste liquid box 3 is located below the guide rails 13. The fixed seat 21 is provided with two slide holes, and the guide rails 13 are arranged in the slide holes. The first vertical plate 11 and the second vertical plate 12 are respectively located at the two ends of the waste liquid box 3, and the second vertical plate 12 is located close to one end of the mounting column 41, and the top surface of the second vertical plate 12 is lower than the groove bottom of the fixed groove 211, so that when the fixed seat 21 slides on the guide rails 13, the glass slide 81 will not touch the vertical plate, avoiding the damage of the glass slide 81. The second vertical plate 12 is also provided with a limiting part 121, which is used to limit the position of the fixed seat 21 moving on the guide rails 13 towards the mounting column 41, to ensure that the glass slide 81 will not touch the mounting column 41, effectively avoiding the glass slide 81 being broken by the mounting column 41.

[0032] As Figures 5 to 8As shown, the first fixing mechanism 6 includes a fixing plate 61, a mounting plate 62 and a connecting seat 63, the fixing plate 61 is fixed on the mounting plate 62, the fixing plate 61 is provided with a non-slip pad 64 for increasing friction to prevent the push plate 82 from falling out of the positioning gap 621. The mounting plate 62 is hinged with the connecting seat 63 through a hinge, the mounting plate 62 is provided with a positioning gap 621 for positioning the push plate 82, the positioning gap 621 has a first positioning edge 6211 and a second positioning edge 6212, the first positioning edge 6211 is perpendicular to the second positioning edge 6212, the first positioning edge 6211 is aligned with the side corresponding to the fixing groove 211, so that the push plate 82 can be aligned with the glass slide 81, and the second positioning edge 6212 is used to limit the distance between the push plate 82 and the glass slide 81, i.e. to limit the thickness of the blood film. The bottom surface of the positioning gap 621 is provided with a sponge pad 65, so that when the push plate 82 is placed, the sponge pad 65 can be squeezed by the push plate 82 to form a gap between the sponge pad 65 and the pressing plate, facilitating the positioning of the push plate 82 into the positioning gap 621, after the push plate 82 is moved to be in close contact with the first positioning edge 6211 and the second positioning edge 6212, the push plate 82 is released, and the push plate 82 is in close contact with the non-slip pad 64 under the action of the sponge, thereby realizing anti-falling. Of course, the fixing plate 61 can also be clamped to the push plate 82 by using the return spring 23, the connecting rod 24 and the limiting plate 25 as in the clamp plate 22 of the fixator 2. The surface of the mounting plate 62 and the connecting seat 63 is inclined, which ensures that the inclination angle of the mounting plate 62 meets the requirements, so as to ensure that the included angle between the push plate 82 and the glass slide 81 is within the required range. When the push plate 82 is not needed, the mounting plate 62 can also be turned over to tilt the mounting plate 62 towards the center of the turntable 42, which will not affect the blood film or blood during the rotation of the turntable 42.

[0033] The second fixing mechanism 71 and the third fixing mechanism 72 are both provided with perforations, and the liquid drop tubes of the dyeing agent box 83 and the buffer box 84 all extend through the perforations, so as to facilitate the dropping of the dyeing agent and the buffer onto the blood smear.

[0034] As Figure 9As shown, the mounting column 41 is provided with a positioning pin 91, the rotating disc 42 is provided with a positioning sleeve provided with a positioning hole, the positioning pin 91 cooperates with the positioning hole to realize the positioning of the rotating disc 42. Specifically, the mounting column 41 is provided with a containing hole, the positioning pin 91 is arranged in the containing hole, the containing hole is provided with a positioning spring 92, the positioning spring 92 abuts on the positioning pin 91 and the bottom of the containing hole, the containing hole is screw provided with a sealing plug 93, the sealing plug 93 is provided with a through hole, part of the positioning pin 91 is exposed from the through hole, that is, part of the positioning pin 91 is located outside the mounting column 41, which is convenient for cooperating with the positioning hole. The rotating of the rotating disc 42 will extrude the positioning pin 91, when the positioning pin 91 is aligned with the positioning hole, the positioning pin 91 is reset under the action of the positioning spring 92, so that the positioning pin 91 is inserted into the positioning hole, and the positioning is realized.

[0035] In use, the limiting plate 25 is pushed to move upward to make the clamping plate 22 follow the upward movement, the glass slide 81 with blood drops is placed in the fixing groove 211 of the fixing device 2, and then the limiting plate 25 is released, so that the clamping plate 22 clamps the glass slide 81 on the fixing seat 21 by the reset spring 23. Then the fixing seat 21 is pushed to move on the guide rail 13 to the direction of the rotating disc 42, and after moving to a predetermined position, the rotating disc 42 is rotated to align the first fixing mechanism 6 with the glass slide 81, that is, the push piece is aligned with the glass slide 81, if the mounting plate 62 is in the unturned state at this time, the end of the push piece is just located behind the blood, if the mounting plate 62 is in the turned state at this time, the mounting plate 62 is turned over, then the fixing seat 21 is moved to make the end of the push piece contact the blood, after the blood diffuses along the edge of the push piece, the fixing seat 21 is pushed away from the rotating disc 42 to form a blood film on the glass slide 81, and after the blood film is dried, a blood smear is formed. The rotating disc 42 is rotated again to move the second fixing mechanism 71 to an operation position, and the blood smear is pushed to a predetermined position, so that the dyeing agent box 83 is squeezed to squeeze the dyeing agent on the blood smear, while the dyeing agent box 83 is squeezed, it is slowly moved away from the rotating disc 42 to ensure that the dyeing agent is attached to the blood smear; after 30s, the rotating disc 42 is rotated to move the second fixing mechanism 71 to an operation position, and the buffer solution is squeezed to drop on the blood smear; after 5 minutes, the cleaner 5 is opened to clean the blood smear, and the cleaning is completed.

[0036] The above is only an embodiment of the present application, and does not limit the patent range of the present application, and any equivalent structure, direct or indirect application in other related technical fields according to the content of the present application, is also within the patent protection range of the present application.

Claims

1. A blood smear staining device, characterized by: The fixing device includes a fixing device for fixing a slide, a guide frame for guiding the moving direction of the fixing device, a first fixing mechanism for installing a push plate, a second fixing mechanism for installing a staining agent box, a third fixing mechanism for installing a buffer box, a rotating disc for installing the first fixing mechanism, the second fixing mechanism and the third fixing mechanism, a mounting column for installing the rotating disc, a cleaning device for cleaning, and a waste liquid box for collecting waste liquid, the fixing device is slidingly arranged on the guide frame, the rotating disc is rotationally arranged on the mounting column, the rotating disc is rotated to adjust the alignment of the first fixing mechanism, the second fixing mechanism or the third fixing mechanism with the fixing device, and the waste liquid box is located below the guide frame.

2. A blood smear staining device as claimed in claim 1, wherein: The fixing device includes a fixing seat for placing a slide, a clamping plate for clamping the slide on the fixing seat, a connecting rod for connecting with the clamping plate, a reset spring for resetting the clamping plate and applying a clamping force to the clamping plate, and a limiting plate for limiting the reset spring, the connecting rod passes through the fixing seat and is fixed with the limiting plate, and the two ends of the reset spring abut against the fixing seat and the limiting plate.

3. A blood smear staining device as claimed in claim 2, wherein: The fixing seat is provided with a fixing groove for fixing the slide, and the depth of the fixing groove is less than the thickness of the slide.

4. A blood smear staining device as claimed in claim 3, wherein: The guide frame includes a guide slide for guiding the fixing seat, and a first vertical plate and a second vertical plate for installing the guide slide, the waste liquid box is located below the guide slide, and the first vertical plate and the second vertical plate are respectively located at both ends of the waste liquid box.

5. A blood smear staining device as claimed in claim 4, wherein: The second vertical plate is located near one end of the mounting column, and the top surface of the second vertical plate is lower than the groove bottom of the fixing groove.

6. A blood smear staining device as claimed in claim 4, wherein: The second vertical plate is further provided with a limiting portion for limiting the fixing seat.

7. A blood smear staining device as claimed in claim 2, wherein: The two ends of the limiting plate protrude from the fixing seat.

8. A blood smear staining device as in claim 1, wherein: The first fixing mechanism includes a mounting plate for placing a push plate, a fixing plate for fixing the push plate, and a connecting seat for connecting the mounting plate with the rotating disc, the mounting plate is hingedly connected with the connecting seat, the mounting plate is provided with a positioning notch, the bottom surface of the positioning notch is provided with a sponge pad, and the fixing plate is provided with an anti-slip pad for increasing friction.

9. A blood smear staining device as claimed in claim 8, wherein: The positioning notch has a first positioning edge and a second positioning edge, the first positioning edge is used to define the alignment of the push plate with the slide, and the second positioning edge is used to limit the distance between the push plate and the slide.

10. A blood smear staining device as in claim 1, wherein: The mounting column is provided with a containing hole, the containing hole is provided with a positioning pin and a positioning spring, the positioning pin abuts against the spring, a sealing plug is screwedly arranged on the containing hole, part of the positioning pin is exposed from the sealing plug, the rotating disc is provided with a positioning sleeve, and the positioning sleeve is provided with a positioning hole matched with the positioning pin.

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