Rapid biofilm culturing device for bacteria-algae membrane

Abstract

The utility model provides a fast biofilm culturing device for bacteria and algae membranes, which relates to the field of water treatment and comprises a culture tank (10), a cover plate (20) and an expanding culture-biofilm culturing reactor (30), first reflective layers (11) are uniformly arranged on the side wall of an inner cavity of the culture tank, and first luminous bodies (12) are uniformly arranged on the side surface of one side, far away from the culture tank (10), of each first reflective layer (11); a limiting ring (21) and a sealing ring (22) are arranged on the bottom surface of the cover plate (20); a second light reflecting layer (23) and a second luminous body (24) are arranged on the inner side of the limiting ring (21); the expanding culture-biofilm culturing reactors (30) are uniformly arranged in the inner cavity of the culturing tank (10), and a third luminous body (13) is arranged between every two adjacent expanding culture-biofilm culturing reactors (30). The device can realize rapid biofilm formation of microalgae, the biofilm formation time is short, the biofilm formation condition is controllable, and the treatment effect and efficiency of the circulating aquaculture water body are effectively improved.

Description

Technical Field

[0001] This utility model relates to the field of water treatment technology, specifically to a rapid biofilm attachment device for bacteria and algae. Background Technology

[0002] Currently, biochemical denitrification in recirculating aquaculture systems mainly relies on nitrification. Nitrification is the process by which ammonia is oxidized to nitrate by microorganisms, thus achieving nitrogen cycling. Common bacterial genera for nitrification are nitrite-oxidizing bacteria and nitrifying bacteria; both are autotrophic bacteria with long growth cycles, typically requiring about three weeks to complete biofilm formation on the substrate. This makes them unsuitable for rapid application in recirculating aquaculture systems, thus affecting the system's water treatment efficiency and effectiveness.

[0003] Algal-microbe symbiotic water treatment is a newly emerging low-energy wastewater treatment technology in recent years. It removes nitrogen, phosphorus, and organic matter from water through the mutual promotion between bacteria and algae, thereby purifying the water. However, due to the small size and density of microalgae, they mostly exist in suspended form in water bodies, making them prone to being washed away by water flow, resulting in the loss of algal cells. This affects the biofilm formation efficiency and prolongs the biofilm formation time, and also reduces the synergistic effect between bacteria and algae, thus impacting the water treatment effect. Utility Model Content

[0004] To address the problems existing in the prior art, the purpose of this utility model is to provide a rapid algal biofilm formation device. This device enables the rapid formation of microalgae biofilms, promoting bacterial growth through algae and utilizing the synergistic effect of bacteria and algae to achieve rapid biofilm formation. The biofilm formation time is short and the biofilm formation conditions are controllable, thereby improving the treatment effect and efficiency of recirculating aquaculture water and ensuring the timeliness of water treatment.

[0005] The objective of this utility model is achieved through the following technical solution:

[0006] A rapid biofilm formation device for bacteria and algae includes a culture tank, a cover plate, and a propagation-biofilm formation reactor. The culture tank has no cover at the top, and a first reflective layer is uniformly arranged on the inner wall of the tank. A first light-emitting element is uniformly arranged on the side of the first reflective layer away from the culture tank. A coaxial limiting ring is fixedly arranged on the bottom surface of the cover plate corresponding to the inner cavity of the culture tank, and a sealing ring is fixedly arranged on the outer wall of the limiting ring. A second reflective layer is fixedly arranged on the bottom surface of the cover plate inside the limiting ring, and a second light-emitting element is uniformly arranged on the lower side of the second reflective layer. The propagation-biofilm formation reactor is uniformly arranged in the inner cavity of the culture tank and located between the first light-emitting elements and below the second light-emitting elements. A third light-emitting element is arranged between adjacent propagation-biofilm formation reactors.

[0007] Based on further optimization of the above scheme, the size of the cover plate is larger than the size of the culture tank (that is, the length and width of the cover plate are larger than the length and width of the outer wall of the culture tank, respectively); the outer wall size of the limiting ring is smaller than the inner cavity size of the culture tank (that is, the outer wall length and width of the limiting ring are smaller than the inner cavity size of the culture tank, respectively).

[0008] Based on further optimization of the above scheme, the first light-emitting body, the second light-emitting body, and the third light-emitting body can be any one of the light-emitting devices composed of light-emitting tubes, light-emitting strips, sunlight guides, and light guides.

[0009] Based on further optimization of the above scheme, the expansion-film reactor includes two transparent plexiglass pieces, a filter screen, and a culture medium. The two transparent plexiglass pieces are arranged in parallel with a gap between them. The gap between the two transparent plexiglass pieces is filled with the culture medium. A filter screen is set between the two ends and the top of the opposite sides of the two transparent plexiglass pieces. This filter screen serves three purposes: first, to connect the two transparent plexiglass pieces; second, to fix and limit the culture medium in the gap; and third, to facilitate the flow of water.

[0010] Based on further optimization of the above scheme, the height of the propagation-film reactor is not less than the height of the third luminescent body.

[0011] Based on further optimization of the above scheme, the rapid biofilm attachment device for bacteria and algae also includes a heating and heat preservation component. The heating and heat preservation component includes a heat preservation shell, support ribs and a circulating heating component. The top surface of the heat preservation shell has a through hole corresponding to the culture tank and the support ribs are evenly arranged at the bottom of the inner cavity of the heat preservation shell. The circulating heating component is located on the outside of the heat preservation shell and is connected to the inner cavity of the heat preservation shell.

[0012] Based on further optimization of the above scheme, thermistors are uniformly arranged in the inner cavity of the culture tank for real-time monitoring of the temperature inside the culture tank.

[0013] The following are the technical effects of this utility model:

[0014] This device utilizes the combined effects of a first, second, and third light-emitting body, a first reflective layer, a second reflective layer, and a propagation-film formation reactor to provide strong supplemental lighting for microalgae, enabling rapid proliferation and propagation within a short timeframe. This allows for the rapid (generally around 5 days) colonization and film formation of microalgae on the substrate surface. The propagation-film formation reactor, composed of translucent plexiglass, a filter, and the culture medium, not only facilitates water flow and light penetration, promoting rapid microalgae proliferation, but also immobilizes and confines the culture medium, ensuring successful film formation and preventing loss due to water flow, thus guaranteeing optimal cultivation results. This device effectively shortens the microalgae formation time and ensures uniform film formation. Furthermore, in subsequent water treatment, the synergistic effect of bacteria and algae, promoting bacterial growth through algae, leads to rapid maturation of the algal film, enabling rapid, timely, and efficient treatment of recirculating aquaculture water. The device's controllable film formation conditions make it suitable for treating various water types and have a wide range of applications. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of the rapid film-attaching device in this utility model.

[0016] Figure 2 for Figure 1 A magnified view of part A in the image.

[0017] Figure 3 This is a schematic diagram of the expansion-film-film reactor of the rapid film-film-attaching device in this utility model.

[0018] Among them, 10, culture tank; 11, first reflective layer; 12, first light-emitting body; 13, third light-emitting body; 20, cover plate; 21, limiting ring; 22, sealing ring; 23, second reflective layer; 24, second light-emitting body; 30, expansion-film reactor; 31, transparent plexiglass; 32, filter screen; 33, culture substrate; 41, heat-insulating shell; 42, supporting ridge. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0020] Example 1:

[0021] A rapid biofilm attachment device for bacteria and algae includes a culture tank 10, a cover plate 20, and a propagation-biofilm attachment reactor 30. The culture tank 10 has no cover on top, and a first reflective layer 11 is uniformly arranged on the inner sidewall of the culture tank 10 (i.e., the first reflective layer 11 is attached to the inner sidewall of the culture tank 10). A first light-emitting body 12 is uniformly arranged on the side of the first reflective layer 11 away from the culture tank 10 (the first light-emitting body 12 is any one of the light-emitting devices composed of a light tube, a light strip, a sunlight guide, and a light guide; if a light tube is used, the light tube is uniformly fixed on the sidewall of the first reflective layer 11 at a certain interval and the light tube is vertically arranged; if a light strip is used, it can be evenly laid on the sidewall of the first reflective layer 11).

[0022] A coaxial limiting ring 21 is fixedly installed on the bottom surface of the cover plate 20 corresponding to the inner cavity of the culture tank 10 (i.e., the limiting ring 21 is coaxially set with the cover plate 20, such as...). Figure 1 As shown), a sealing ring 22 is fixedly installed on the outer wall of the limiting ring 21 (the outer wall of the sealing ring 22 fits against the inner wall of the culture tank 10 to prevent water from overflowing). A second reflective layer 23 is fixedly installed on the bottom surface of the cover plate 20 inside the limiting ring 21 (the second reflective layer 23 and the first reflective layer 11 use the same reflective material, such as a reflector or a reflector). A second light-emitting body 24 is evenly arranged on the lower side of the second reflective layer 23 (the second light-emitting body 24 is any one of the light-emitting devices composed of a light tube, a light strip, a sunlight guide, and a light guide; if a light tube is used, the light tube is fixedly connected to the inner walls of both sides of the limiting ring 21 at both ends and the light is evenly arranged; if a light strip is used, it is evenly laid on the bottom surface of the second reflective layer 23). The size of the cover plate 20 is larger than the size of the culture tank 10 (that is, the length and width of the cover plate 20 are larger than the length and width of the outer wall of the culture tank 10, respectively). Figure 1 As shown); the outer wall dimension of the limiting ring 21 is smaller than the inner cavity dimension of the culture tank 10 (that is, the length and width of the outer wall of the limiting ring 21 are smaller than the length and width of the inner cavity of the culture tank 10, respectively, as shown). Figure 1 (as shown), thus facilitating the stable mounting of the cover plate 20 on the top of the culture tank 10.

[0023] The expansion-film reactor 30 is evenly arranged in the inner cavity of the culture tank 10 (e.g., Figure 1 As shown, the expansion-film formation reactor 30 is arranged in a blade-like array at the bottom of the inner cavity of the culture tank 10 and is located between the first light emitters 12 and below the second light emitters 24. A third light emitter 13 (which can be any one of a light-emitting device composed of a light tube, a light strip, a sunlight guide, and a light guide) is arranged between adjacent expansion-film formation reactors 30. The expansion-film formation reactor 30 includes two pieces of transparent plexiglass 31, a filter screen 32, and a culture medium 33. The two pieces of transparent plexiglass 31 are arranged in parallel with a gap between them. The gap between the two pieces of transparent plexiglass 31 is filled with the culture medium 33 (combined with...). Figure 2 and Figure 3As shown), a filter 32 is provided between the two ends and the top of the opposite sides of the two pieces of translucent organic glass 31 (as shown). Figure 3 As shown), the purpose is threefold: first, to connect the two pieces of transparent plexiglass 31; second, to fix and limit the culture medium 33 in the gap; and third, to facilitate water flow. The height of the expansion-film reactor 30 is not less than the height of the third luminescent body 13.

[0024] To ensure the temperature during the biofilm formation process, the rapid biofilm formation device also includes a heating and insulation component. The heating and insulation component includes an insulation shell 41, support ribs 42, and a circulating heating component. The top surface of the insulation shell 41 has a through hole corresponding to the culture tank 10 (the inner wall of the through hole is fitted with a sealing ring for sealing the water after contact with the outer wall of the culture tank 10). Support ribs 42 are evenly arranged at the bottom of the inner cavity of the insulation shell 41 (the length of the support ribs 42 is less than the width of the insulation shell 41, thereby ensuring the flow of circulating heated water in the cavity between the insulation shell 41 and the culture tank 10). The circulating heating component is located on the outside of the insulation shell 41 and is connected to the inner cavity of the insulation shell 41 (the circulating heating component adopts a structure composed of a circulating water pump, a heating core, and a connecting pipe, or it can adopt a structure similar to a gas water heater or boiler, to provide circulating hot water to the inner cavity of the insulation shell 41, thereby realizing the heating and insulation of the culture tank 10). Thermistors (thermistors of conventional models in the field that can accurately measure the temperature inside the culture tank 10) are uniformly arranged in the inner cavity of the culture tank 10 for real-time monitoring of the temperature inside the culture tank 10.

[0025] Example 2:

[0026] As another preferred embodiment of this utility model, in order to ensure the stable positioning of the expansion-film reactor 30, based on the above embodiment 1, slots are respectively opened on the bottom surface of the inner cavity of the culture tank 10 and the light-transmitting plexiglass 31 corresponding to the expansion-film reactor 30. There is a certain distance between the bottom of the side wall filter 32 of the expansion-film reactor 30 and the bottom surface of the light-transmitting plexiglass 31 (this distance is the slot depth), thereby realizing the connection between the expansion-film reactor 30 and the culture tank 10; at the same time, the top filter 32 of the expansion-film reactor 30 is set as a movable structure, thereby facilitating the filling of the culture substrate 33.

[0027] Working principle:

[0028] First, the microalgae culture medium and culture substrate 33 are filled into the gaps of the expansion-film formation reactor 30. Simultaneously, the first luminescent body 12, the second luminescent body 24, and the third luminescent body 13 are activated. The light generated by the luminescent bodies is transmitted through the transparent plexiglass 31 and irradiates the culture substrate 33. At the same time, it is further irradiated onto the culture substrate 33 by the reflection of the first reflective layer 11 and the second reflective layer 23, forming strong supplemental lighting. During this process, hot water is introduced to heat and keep the culture tank 10 warm, so that the temperature inside the culture tank 10 is stabilized between 25 and 30°C, allowing the microalgae to quickly colonize and form a film on the culture substrate 33. Microalgae colonization and biofilm devices can remove a small amount of ammonia nitrogen in the early stages of a recirculating aquaculture system. After the microalgae biofilm is established, it is transferred to a biological denitrification system. By utilizing the synergistic effect of bacteria and algae and promoting bacterial growth through algae, the biofilm matures rapidly. The biofilm obtained using this method takes about 7 to 10 days, which is significantly shorter than the 2 to 3 weeks required for biofilm formation through traditional nitrification.

[0029] Example 3:

[0030] As another preferred embodiment of this utility model, based on the above embodiment 1, a rubber buffer pad is provided on the top surface of the culture tank 10 to buffer the cover plate 20 when it is closed.

[0031] Example 4:

[0032] As another preferred embodiment of this utility model, based on the above embodiment 1, the culture tank 10 is provided with a water inlet channel and a drainage channel on the outer wall of the upper side of the heat preservation shell 41 for the inflow and outflow of water.

Claims

1. A rapid biofilm attachment device for bacteria and algae, characterized in that: The system includes a culture tank, a cover plate, and a propagation-film formation reactor. The culture tank has no cover at the top and a first reflective layer is uniformly arranged on the inner wall of the tank. A first light-emitting element is uniformly arranged on the side of the first reflective layer away from the culture tank. A coaxial limiting ring is fixedly arranged on the bottom surface of the cover plate corresponding to the inner cavity of the culture tank, and a sealing ring is fixedly arranged on the outer wall of the limiting ring. A second reflective layer is fixedly arranged on the bottom surface of the cover plate inside the limiting ring, and a second light-emitting element is uniformly arranged on the lower side of the second reflective layer. The propagation-film formation reactors are uniformly arranged in the inner cavity of the culture tank and located between the first light-emitting elements and below the second light-emitting elements. A third light-emitting element is arranged between adjacent propagation-film formation reactors.

2. The rapid biofilm attachment device for bacteria and algae according to claim 1, characterized in that: The size of the cover plate is larger than the size of the culture tank; the outer wall size of the limiting ring is smaller than the inner cavity size of the culture tank.

3. The rapid biofilm attachment device for bacteria and algae according to claim 1, characterized in that: The first light source, the second light source, and the third light source are any one of the light-emitting devices composed of a light tube, a light strip, a sunlight guide, and a light guide.

4. The rapid biofilm attachment device for bacteria and algae according to claim 1, characterized in that: The propagation-film reactor includes two transparent plexiglass pieces, a filter screen, and a culture medium. The two transparent plexiglass pieces are arranged in parallel with a gap between them. The gap between the two transparent plexiglass pieces is filled with the culture medium. A filter screen is placed between the two ends and the top of the opposite sides of the two transparent plexiglass pieces.

5. The rapid biofilm attachment device for bacteria and algae according to claim 1, characterized in that: The height of the propagation-film reactor is not less than the height of the third luminescent body.

6. The rapid biofilm attachment device for bacteria and algae according to claim 1, characterized in that: The rapid biofilm attachment device for bacteria and algae also includes a heating and insulation component, which includes an insulation shell, support ribs, and a circulating heating component. The top surface of the insulation shell has a through hole corresponding to the culture tank, and support ribs are evenly arranged at the bottom of the inner cavity of the insulation shell. The circulating heating component is located on the outside of the insulation shell and is connected to the inner cavity of the insulation shell.

7. The rapid biofilm attachment device for bacteria and algae according to claim 6, characterized in that: Thermistors are uniformly arranged inside the culture tank to monitor the temperature inside the culture tank in real time.

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