Serum-free culture medium for animal cells
By designing a serum-free culture medium system with temperature monitoring and circulating cooling modules, the problem of serum-free culture medium deteriorating under high temperature conditions was solved, and effective cooling protection of the culture medium was achieved.
Patent Information
- Application Number
- CN202520034093.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-08
- Publication Date
- 2026-01-23
- Estimated Expiration
- 2035-01-08
AI Technical Summary
Serum-free culture media are prone to deterioration at high temperatures, and it is difficult to provide a cool environment during handling, which leads to a decline in the quality of the culture medium.
A serum-free culture medium system was designed, comprising a bottle body, a base, and a circulating cooling module. The temperature inside the bottle is monitored in real time by a temperature monitoring module, and the circulating cooling module is activated to use cooling oil to cool down the culture medium, ensuring that the culture medium maintains a suitable temperature in a high-temperature environment.
It effectively prevents high-temperature deterioration of serum-free cultured genes, provides a stable temperature environment, ensures the quality of the culture medium, and is convenient for operators to use.
Smart Images
Figure CN223823607U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of biological laboratory consumables, and in particular to a serum-free culture medium for animal cells. Background Technology
[0002] Serum-free culture media and reagents are widely used to culture mammalian and invertebrate cells to prepare monoclonal antibodies, viral antigens, and recombinant proteins. The basic formula of serum-free culture media consists of two main parts: the basic culture medium and the added components. When cells used for biopharmaceutical and vaccine production are cultured in vitro, most of them exhibit adherent or facultative adherent growth. However, when they grow in serum-free and protein-free culture media, due to the lack of various adhesion factors in serum, such as fibronectin, laminin, collagen, and vitreous adhesion proteins, the cells often grow in suspension.
[0003] When using serum-free animal cell culture media, it is usually necessary to store them in a dry and cool environment, as high ambient temperatures can easily cause the serum-free culture media to deteriorate. However, when taking out the serum-free culture media, it is often necessary to remove the bottle containing the serum-free culture media from the constant temperature storage room, and then put the remaining bottle of serum-free culture media back into the storage room after use. It is difficult to provide a cool environment for the serum-free culture media during the process of taking out the serum-free culture media. Utility Model Content
[0004] In order to continuously provide a cool environment for the serum-free culture medium during the handling of the serum-free culture medium, this application provides a serum-free culture medium for animal cells.
[0005] The serum-free culture medium for animal cells provided in this application adopts the following technical solution:
[0006] A serum-free culture medium for animal cells includes a bottle body and a base. The bottle body includes a bottle mouth, on which a bottle stopper is detachably installed. A placement groove is fixedly installed on the base, and a sandwich layer is provided in the placement groove. The sandwich layer is filled with cooling oil.
[0007] The placement tank is equipped with a liquid outlet pipe and a liquid return pipe. The inlet end of the liquid outlet pipe and the outlet end of the liquid return pipe are both connected to the interlayer. A circulating refrigeration module is fixedly installed on the base. The outlet end of the liquid outlet pipe and the inlet end of the liquid return pipe are both connected to the circulating refrigeration module.
[0008] A temperature monitoring module is fixedly installed inside the bottle stopper, and the signal output terminal of the temperature monitoring module is connected to the signal input terminal of the circulating refrigeration module.
[0009] By adopting the above technical solution, after the user takes out the bottle containing serum-free culture medium, the bottle can be placed in the placement slot on the base and the bottle stopper can be installed on the bottle mouth. When the ambient temperature is too high, the temperature monitoring module controls the circulation cooling module to start, and the cooling oil flowing in the interlayer of the placement slot cools the bottle, providing a good temperature environment for the serum-free culture medium in the bottle, and preventing the serum-free culture medium in the bottle from deteriorating due to excessive temperature.
[0010] Preferably, the stopper includes a stopper body, the inner wall of the bottle opening is provided with an internal thread, and the outer wall of the stopper body is provided with an external thread.
[0011] By adopting the above technical solution, the stopper can be detachably installed at the bottle mouth via a threaded connection, making it convenient for operators to remove the stopper to access serum-free culture medium.
[0012] Preferably, the bottle stopper has an installation cavity, and the temperature monitoring module includes:
[0013] A temperature sensor is fixedly installed inside the mounting cavity, with its sensing end penetrating the bottom of the mounting cavity and extending into the bottle body;
[0014] The processor is fixedly installed inside the mounting cavity. Its signal input terminal is connected to the temperature sensor via a wireless network module, and its signal output terminal is connected to the signal output terminal of the circulating refrigeration module via a wireless network module.
[0015] By adopting the above technical solution, the storage temperature of serum-free culture medium in the bottle can be monitored in real time through temperature sensors and processors. When the temperature in the bottle exceeds the set temperature threshold, the processor controls the circulating cooling module to cool the bottle.
[0016] Preferably, a raised ring is integrally formed on the inner wall of the mounting cavity, and the raised ring is located above the temperature sensor and the processor;
[0017] Activated carbon is detachably mounted on the convex ring, and a cap is detachably mounted on the top of the bottle stopper. The activated carbon is pressed against the cap and the convex ring.
[0018] By adopting the above technical solution, the temperature sensor, wireless network module, processor, etc. inside the installation cavity can be sealed by the cover. The activated carbon inside the installation cavity can absorb the moisture in the air, providing a dry environment for the temperature sensor, processor, and wireless network module.
[0019] Preferably, a plug is fixedly connected to the bottom of the cap, the plug is provided with external threads, the mounting cavity is provided with internal threads, the plug is threadedly installed in the mounting cavity, and the bottom of the plug abuts against the top of the activated carbon.
[0020] By adopting the above technical solution, the external thread on the plug and the internal thread in the installation cavity can achieve the technical effect of detachable installation of the cover in the installation cavity through threaded connection.
[0021] Preferably, the circulating cooling module includes a circulating pump and a semiconductor cooling mechanism. The semiconductor cooling mechanism includes a liquid storage tank and a cooling semiconductor. A mounting base is fixedly installed on the outer wall of the liquid storage tank, and the cooling semiconductor is fixedly installed in the mounting base.
[0022] A thermally conductive graphite plate is fixedly installed in the mounting base. One side of the thermally conductive graphite plate is in contact with the cold end of the cooling semiconductor. A plurality of conductive protrusions are formed on the side of the thermally conductive graphite plate away from the cooling semiconductor, and the plurality of conductive protrusions extend into the liquid storage tank.
[0023] The outlet end of the liquid outlet pipe is connected to the liquid storage tank, the inlet end of the liquid return pipe is connected to the liquid storage tank, and the circulation pump is installed on the liquid return pipe.
[0024] The signal input terminals of the circulating pump and the cooling semiconductor are both connected to the signal output terminal of the processor via a wireless network module.
[0025] By adopting the above technical solution, when the temperature inside the bottle is too high, the circulation pump starts and transports the coolant in the jacket to the storage tank through the outlet pipe. The cooling semiconductor generates cold energy to cool the cooling oil circulating through the storage tank. The cooled cooling oil flows back to the jacket through the return pipe, which lowers the temperature of the bottle and thus the temperature inside the bottle, providing a good temperature environment for the serum-free culture medium inside the bottle. The use of a thermally conductive graphite plate, due to its excellent heat dissipation performance, improves the efficiency of heat exchange between the cooling oil and the cold end of the cooling semiconductor, enhancing the cooling effect on the cooling oil.
[0026] Preferably, a cooling fan is fixedly mounted on the mounting base, the cooling fan is opposite to the hot end of the cooling semiconductor, and the signal input end of the cooling fan is connected to the signal output end of the processor through a wireless network module.
[0027] By adopting the above technical solution, the cooling fan can disperse the heat accumulated at the hot end of the cooling semiconductor.
[0028] In summary, the serum-free culture medium for animal cells proposed in this application has at least one of the following beneficial technical effects:
[0029] 1. When the temperature inside the bottle is too high, the circulation pump starts to transport the coolant in the jacket to the storage tank through the outlet pipe. The cooling semiconductor generates cold energy to cool the cooling oil circulating through the storage tank. The cooled cooling oil flows back to the jacket through the return pipe, which can lower the temperature of the bottle and thus provide a good temperature environment for the serum-free culture medium inside the bottle.
[0030] 2. By installing activated carbon inside the cavity, moisture in the air can be adsorbed, providing a dry environment for the temperature sensor, processor, and wireless network module. Attached Figure Description
[0031] Figure 1 This is a schematic diagram illustrating the overall structure of the serum-free culture medium in the embodiments of this application.
[0032] Figure 2 This is a schematic diagram illustrating the signal transmission between the temperature monitoring module and the circulating cooling module, as shown in the embodiments of this application.
[0033] Explanation of reference numerals in the attached diagram: 1. Bottle body; 11. Bottle mouth; 2. Base; 21. Placement groove; 22. Interlayer; 23. Liquid outlet pipe; 24. Liquid return pipe; 3. Bottle stopper; 31. Stopper body; 32. Mounting cavity; 33. Protruding ring; 34. Activated carbon; 35. Cap; 36. Plug; 4. Circulating refrigeration module; 41. Circulating pump; 42. Liquid storage tank; 43. Refrigeration semiconductor; 44. Thermally conductive graphite plate; 45. Cooling fan; 5. Temperature sensor. Detailed Implementation
[0034] The following is in conjunction with the appendix Figure 1-2 This application will be described in further detail.
[0035] Example
[0036] This application discloses a serum-free culture medium for animal cells. (Refer to...) Figures 1-2 It mainly includes a bottle body 1 and a base 2. The bottle body 1 includes a bottle mouth 11, on which a bottle stopper 3 is detachably installed. A placement groove is fixedly installed on the base 2, and a jacket 21 is provided in the placement groove, which is filled with cooling oil. An outlet pipe 23 and a return pipe 24 are provided on the placement groove. The inlet end of the outlet pipe 23 and the outlet end of the return pipe 24 are both connected to the jacket 21. A circulating refrigeration module 4 is fixedly installed on the base 2, and the outlet end of the outlet pipe 23 and the inlet end of the return pipe 24 are both connected to the circulating refrigeration module 4. A temperature monitoring module is fixedly installed in the bottle stopper 3, and the signal output end of the temperature monitoring module is connected to the signal input end of the circulating refrigeration module 4.
[0037] After the user removes the bottle 1 containing serum-free culture medium, the bottle 1 can be placed in the placement slot on the base 2, and the bottle stopper 3 can be installed on the bottle mouth 11. When the ambient temperature is too high, the temperature monitoring module controls the circulation cooling module 4 to start, and cools the bottle 1 by the cooling oil flowing in the interlayer 21 of the placement slot, so as to provide a good temperature environment for the serum-free culture medium in the bottle 1 and prevent the serum-free culture medium in the bottle 1 from deteriorating due to excessive temperature.
[0038] Reference Figure 1 The bottle stopper 3 includes a stopper body 31, with an internal thread on the inner wall of the bottle mouth 11 and an external thread on the outer wall of the stopper body 31.
[0039] The stopper 31 can be detachably installed at the bottle mouth 11 of the bottle body 1 by means of a threaded connection, which makes it convenient for the operator to remove the stopper 3 to take out the serum-free culture medium.
[0040] Reference Figure 1 and Figure 2 In this embodiment, the bottle stopper 3 has an installation cavity 32. The temperature monitoring module includes: a temperature sensor 5, which is fixedly installed in the installation cavity 32, with its sensing end penetrating through the bottom of the installation cavity 32 and extending into the bottle body 1; and a processor, which is fixedly installed in the installation cavity 32, with its signal input end connected to the temperature sensor 5 via a wireless network module, and its signal output end connected to the signal output end of the circulating cooling module 4 via a wireless network module.
[0041] The temperature sensor 5 and the processor can monitor the storage temperature of serum-free culture medium in bottle 1 in real time. When the temperature in bottle 1 exceeds the set temperature threshold, the processor controls the circulating cooling module 4 to cool down bottle 1.
[0042] Reference Figure 1 A raised ring 33 is integrally formed on the inner wall of the mounting cavity 32. The raised ring 33 is located above the temperature sensor 5 and the processor. Activated carbon 34 is detachably installed on the raised ring 33. A cap 35 is detachably installed on the top of the bottle stopper 3. The activated carbon 34 is pressed between the cap 35 and the raised ring 33.
[0043] The temperature sensor 5, wireless network module, processor, etc. inside the mounting cavity 32 can be sealed by the cover 35. The activated carbon 34 inside the mounting cavity 32 can absorb moisture in the air, providing a dry environment for the temperature sensor 5, processor, and wireless network module.
[0044] Referring to the figure, a plug 36 is fixedly connected to the bottom of the cap 35. The plug 36 is provided with external threads, and the mounting cavity 32 is provided with internal threads. The plug 36 is threadedly installed in the mounting cavity 32, and the bottom of the plug 36 abuts against the top of the activated carbon 34.
[0045] By using the external thread on the plug 36 and the internal thread in the mounting cavity 32, the cap 35 can be detachably installed in the mounting cavity 32 through a threaded connection.
[0046] Referring to the figure, the circulating cooling module 4 includes a circulating pump 41 and a semiconductor cooling mechanism. The semiconductor cooling mechanism includes a liquid storage tank 42 and a cooling semiconductor 43. A mounting base is fixedly installed on the outer wall of the liquid storage tank 42, and the cooling semiconductor 43 is fixedly installed in the mounting base. A thermally conductive graphite plate 44 is fixedly installed in the mounting base. One side of the thermally conductive graphite plate 44 is in contact with the cold end of the cooling semiconductor 43. Several conductive protrusions are formed on the side of the thermally conductive graphite plate 44 away from the cooling semiconductor 43, and the conductive protrusions extend into the liquid storage tank 42. The outlet end of the liquid outlet pipe 23 is connected to the liquid storage tank 42, and the inlet end of the liquid return pipe 24 is connected to the liquid storage tank 42. The circulating pump 41 is installed on the liquid return pipe 24. The signal input ends of the circulating pump 41 and the cooling semiconductor 43 are both connected to the signal output end of the processor through a wireless network module.
[0047] When the temperature inside the bottle 1 is too high, the circulation pump 41 starts and transports the coolant in the jacket 21 to the storage tank 42 through the outlet pipe 23. The cooling semiconductor 43 generates cooling energy to cool the cooling oil circulating through the storage tank 42. The cooled cooling oil flows back to the jacket 21 through the return pipe 24, which lowers the temperature of the bottle 1 and thus the temperature inside the bottle 1, providing a good temperature environment for the serum-free culture medium inside the bottle 1. The thermally conductive graphite plate 44, with its excellent heat dissipation performance, improves the efficiency of heat exchange between the cooling oil and the cold end of the cooling semiconductor 43, enhancing the cooling effect on the cooling oil.
[0048] Reference Figure 1 and Figure 2 A cooling fan 45 is fixedly mounted on the mounting base. The cooling fan 45 is opposite to the hot end of the cooling semiconductor 43, and the signal input terminal of the cooling fan 45 is connected to the signal output terminal of the processor via a wireless network module. The cooling fan 45 can dissipate the heat accumulated at the hot end of the cooling semiconductor 43.
[0049] The implementation principle of the serum-free culture medium for animal cells in this application embodiment is as follows: When the temperature inside the bottle 1 is too high, the circulation pump 41 starts to transport the coolant in the jacket 21 to the storage tank 42 through the outlet pipe 23. The cooling semiconductor 43 generates cold energy to cool the cooling oil circulating through the storage tank 42. The cooled cooling oil flows back to the jacket 21 through the return pipe 24, which can lower the temperature of the bottle 1, thereby lowering the temperature inside the bottle 1 and providing a good temperature environment for the serum-free culture medium inside the bottle 1.
[0050] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.
Claims
1. A serum-free culture medium for animal cells, characterized in that, The bottle includes a bottle body (1) and a base (2). The bottle body (1) includes a bottle mouth (11) and a bottle stopper (3) is detachably installed on the bottle mouth (11). A placement groove is fixedly installed on the base (2) and a sandwich layer (21) is provided in the placement groove. The sandwich layer (21) is filled with cooling oil. The placement tank is provided with an outlet pipe (23) and a return pipe (24). The inlet end of the outlet pipe (23) and the outlet end of the return pipe (24) are both connected to the interlayer (21). A circulating refrigeration module (4) is fixedly installed on the base (2). The outlet end of the outlet pipe (23) and the inlet end of the return pipe (24) are both connected to the circulating refrigeration module (4). A temperature monitoring module is fixedly installed inside the bottle stopper (3), and the signal output terminal of the temperature monitoring module is connected to the signal input terminal of the circulating refrigeration module (4).
2. The serum-free culture medium for animal cells according to claim 1, characterized in that, The stopper (3) includes a stopper body (31), with an internal thread on the inner wall of the bottle mouth (11) and an external thread on the outer wall of the stopper body (31).
3. The serum-free culture medium for animal cells according to claim 2, characterized in that, The bottle stopper (3) has an installation cavity (32) inside, and the temperature monitoring module includes: A temperature sensor (5) is fixedly installed in the mounting cavity (32), with its sensing end penetrating the bottom of the mounting cavity (32) and extending into the bottle body (1); The processor is fixedly installed in the mounting cavity (32). Its signal input terminal is connected to the temperature sensor (5) via a wireless network module, and its signal output terminal is connected to the signal output terminal of the circulating cooling module (4) via a wireless network module.
4. The serum-free culture medium for animal cells according to claim 3, characterized in that, A protruding ring (33) is integrally formed on the inner wall of the mounting cavity (32), and the protruding ring (33) is located above the temperature sensor (5) and the processor. Activated carbon (34) is detachably installed on the convex ring (33), and a cap (35) is detachably installed on the top of the bottle stopper (3). The activated carbon (34) is pressed against the cap (35) and the convex ring (33).
5. The serum-free culture medium for animal cells according to claim 4, characterized in that, The bottom of the cap (35) is fixedly connected to a plug (36), the plug (36) is provided with an external thread, the mounting cavity (32) is provided with an internal thread, the plug (36) is threadedly installed in the mounting cavity (32), and the bottom of the plug (36) abuts against the top of the activated carbon (34).
6. The serum-free culture medium for animal cells according to claim 3, characterized in that, The circulating cooling module (4) includes a circulating pump (41) and a semiconductor cooling mechanism. The semiconductor cooling mechanism includes a liquid storage tank (42) and a cooling semiconductor (43). A mounting base is fixedly installed on the outer side wall of the liquid storage tank (42), and the cooling semiconductor (43) is fixedly installed in the mounting base. A thermally conductive graphite plate (44) is fixedly installed in the mounting base. One side of the thermally conductive graphite plate (44) is in contact with the cold end of the cooling semiconductor (43). A plurality of conductive protrusions are formed on the side of the thermally conductive graphite plate (44) away from the cooling semiconductor (43). The plurality of conductive protrusions extend into the liquid storage tank (42). The outlet end of the liquid outlet pipe (23) is connected to the liquid storage tank (42), the inlet end of the liquid return pipe (24) is connected to the liquid storage tank (42), and the circulation pump (41) is installed on the liquid return pipe (24). The signal input terminals of the circulating pump (41) and the cooling semiconductor (43) are both connected to the signal output terminal of the processor via a wireless network module.
7. The serum-free culture medium for animal cells according to claim 6, characterized in that, A cooling fan (45) is fixedly installed on the mounting base. The cooling fan (45) is opposite to the hot end of the cooling semiconductor (43), and the signal input end of the cooling fan (45) is connected to the signal output end of the processor through a wireless network module.