Nano-drug preparation device

Abstract

The utility model relates to a nano-drug preparation device which comprises a nano-drug preparation assembly, a buffer vehicle assembly, a lipid phase storage assembly, a water phase storage assembly, a dilution phase storage assembly, a harvesting phase storage assembly and a liquid path assembly. The buffer vehicle assembly is provided with a lipid phase buffer unit, a water phase buffer unit and a dilution phase buffer unit; the output ends of the storage assemblies are connected with the input ends of the buffer units in a one-to-one correspondence manner; the output ends of the buffer units are connected with the input ends of the nano-drug preparation assemblies in a one-to-one correspondence mode, and the output ends of the nano-drug preparation assemblies are connected with the input end of the harvesting phase storage assembly. A peristaltic pump and a centrifugal pump which are arranged on the liquid path assembly are matched to work, so that a lipid phase, a water phase and a dilution phase are respectively conveyed into the nano-drug preparation assembly. The nano-drug preparation device provided by the utility model aims to overcome the existing defects, and has the advantages of convenience in operation, compact structure, advanced, simple and convenient process and accuracy and stability in operation.

Description

Technical Field

[0001] This utility model relates to the field of pharmaceutical technology, and in particular to a nanomedicine preparation device. Background Technology

[0002] Nanomedicine preparation devices are a process that uses microfluidic laminar flow technology to prepare drugs into nanoscale particles. The main goal is to optimize the preparation process and improve the equipment's preparation capabilities to achieve large-scale preparation of nanomedicine particles.

[0003] Currently, the preparation of nanomedicines generally suffers from problems such as high production costs, cumbersome processes, low automation, obstacles to scale-up production, and quality control, which restrict the development of nanomedicines. At present, there is a lack of stable large-scale nanomedicine preparation systems and equipment. Therefore, a nanomedicine preparation device is proposed to address the above problems. Utility Model Content

[0004] The purpose of this invention is to overcome the existing defects and provide a nanomedicine preparation device that has the advantages of convenient operation, compact structure, advanced and simple process, and precise and stable operation.

[0005] The technical solution to achieve the above objective is: a nanomedicine preparation device, comprising: a nanomedicine preparation component, a buffer cart component, a lipid phase storage component, an aqueous phase storage component, a dilution phase storage component, a harvest phase storage component, and a liquid path component;

[0006] The buffer vehicle assembly includes a lipid phase buffer unit, an aqueous phase buffer unit, and a dilution phase buffer unit. The output terminals of the lipid phase storage assembly, the aqueous phase storage assembly, and the dilution phase storage assembly are connected to the input terminals of the lipid phase buffer unit, the aqueous phase buffer unit, and the dilution phase buffer unit, respectively. The output terminals of the lipid phase buffer unit, the aqueous phase buffer unit, and the dilution phase buffer unit are connected to the input terminals of the nanomedicine preparation assembly, and the output terminal of the nanomedicine preparation assembly is connected to the input terminal of the harvest phase storage assembly.

[0007] The fluid circuit assembly is equipped with a first peristaltic pump, a second peristaltic pump, a third peristaltic pump, a first centrifugal pump, a second centrifugal pump, and a third centrifugal pump. The first peristaltic pump is used to transport the lipid phase stored in the lipid phase storage assembly to the lipid phase buffer unit, and the first centrifugal pump is used to transport the lipid phase in the lipid phase buffer unit to the nanomedicine preparation assembly. The second peristaltic pump is used to transport the aqueous phase stored in the aqueous phase storage assembly to the aqueous phase buffer unit, and the second centrifugal pump is used to transport the aqueous phase in the aqueous phase buffer unit to the nanomedicine preparation assembly. The third peristaltic pump is used to transport the dilution phase stored in the dilution phase storage assembly to the dilution phase buffer unit, and the third centrifugal pump is used to transport the dilution phase in the dilution phase buffer unit to the nanomedicine preparation assembly.

[0008] Preferably, the nanomedicine preparation component includes a microfluidic chip, a first sample inlet line, a second sample inlet line, a first line, a sixth line, a fourth peristaltic pump, and a fluid control unit;

[0009] The first centrifugal pump is connected to the first inlet end of the microfluidic chip through the first sample inlet line, the second centrifugal pump is connected to the second inlet end of the microfluidic chip through the second sample inlet line, the outlet end of the microfluidic chip is provided with a four-way connector, the first end of the four-way connector is connected to the outlet end of the microfluidic chip, the third centrifugal pump is connected to the second end of the four-way connector through the first line, the fourth peristaltic pump is connected to the third end of the four-way connector through the peristaltic pump tube, and the fourth end of the four-way connector is connected to the sixth line.

[0010] The fluid control unit includes a check valve, a pinch valve, and a flow sensor disposed on the first injection line, the second injection line, and the first line.

[0011] Preferably, the first centrifugal pump, the second centrifugal pump, and the third centrifugal pump are all magnetic levitation pumps, and two one-way valves are provided on the first sample inlet line, the second sample inlet line, and the first line.

[0012] Preferably, it further includes: a standard solution storage container and a standard solution pipeline, wherein the standard solution pipeline includes a first standard solution connection pipeline, a second standard solution connection pipeline and a third standard solution connection pipeline; one end of each of the first standard solution connection pipeline, the second standard solution connection pipeline and the third standard solution connection pipeline is connected to the outlet of the standard solution storage container; the other end of the first standard solution connection pipeline is connected to the third centrifugal pump through a third pump connector connection pipe; the other end of the second standard solution connection pipeline is connected to the second centrifugal pump through a second pump connector connection pipe; and the other end of the third standard solution connection pipeline is connected to the first centrifugal pump through a first pump connector connection pipe.

[0013] Pipe clamps are provided on the third standard solution connecting pipe, the second standard solution connecting pipe, and the first standard solution connecting pipe.

[0014] Preferably, it also includes a waste liquid collection container and a waste liquid pipeline, wherein the waste liquid pipeline includes a second pipeline, a third pipeline, a fourth pipeline and a fifth pipeline, the fourth pipeline and the fifth pipeline are connected in parallel between the waste liquid collection container and the third pipeline, and the second pipeline and the fourth peristaltic pump are connected in parallel between the third pipeline and the third end of the four-way connector;

[0015] The second pipeline is equipped with a pinch valve, the fourth pipeline is equipped with a mass flow meter, and the fifth pipeline is equipped with a pneumatic ball valve.

[0016] Preferably, a first leakage detection sensor is provided on both the fourth pipeline and the fifth pipeline.

[0017] Preferably, the harvested phase storage assembly includes a second housing, inside which a finished product collection container is disposed, the inlet of which is connected to the sixth pipeline, and the sixth pipeline is provided with a clamp valve and a pipe clamp.

[0018] The buffer vehicle assembly includes a third housing, on the top of which a first weighing sensor, a second weighing sensor, and a third weighing sensor are respectively disposed. The lower end of the first weighing sensor is connected to a dilution phase buffer bag, the lower end of the second weighing sensor is connected to an aqueous phase buffer bag, and the lower end of the third weighing sensor is connected to a lipid phase buffer bag.

[0019] The first, second, and third peristaltic pumps are all disposed on the outer wall of the third housing. The first peristaltic pump is disposed on the lipid phase delivery pipeline and is used to deliver liquid from the lipid phase storage component to the lipid phase buffer bag. The second peristaltic pump is disposed on the aqueous phase delivery pipeline and is used to deliver liquid from the aqueous phase storage component to the aqueous phase buffer bag. The third peristaltic pump is disposed on the dilution phase delivery pipeline and is used to deliver liquid from the dilution phase storage component to the dilution phase buffer bag.

[0020] The outlet of the dilution phase buffer bag is connected to the third centrifugal pump via a third buffer cart connecting pipe and a third pump connector connecting pipe; the outlet of the aqueous phase buffer bag is connected to the second centrifugal pump via a second buffer cart connecting pipe and a second pump connector connecting pipe; the outlet of the lipid phase buffer bag is connected to the first centrifugal pump via a first buffer cart connecting pipe and a first pump connector connecting pipe.

[0021] Pipe clamps are provided on the third buffer car connecting pipe, the second buffer car connecting pipe and the first buffer car connecting pipe.

[0022] The lipid phase storage assembly includes a sixth housing, within which a lipid phase storage bag is disposed, and the outlet of the lipid phase storage bag is connected to the first peristaltic pump;

[0023] The aqueous phase storage assembly includes a fourth housing, an aqueous phase storage bag is disposed inside the fourth housing, and the outlet of the aqueous phase storage bag is connected to the second peristaltic pump;

[0024] The dilution phase storage assembly includes a fifth housing, within which a dilution phase storage bag is disposed, and the outlet of the dilution phase storage bag is connected to the third peristaltic pump.

[0025] The pump heads of the first centrifugal pump, the second centrifugal pump, and the third centrifugal pump, together with sterile connectors, clamps, flow sensors, first tubing, microfluidic chips, four-way connectors, three-way connectors, and peristaltic pump tubing, constitute a disposable consumable kit.

[0026] The inlet of the flow sensor and the outlet of the pump head are fixed together by the clamp, and the tee is used to connect the peristaltic pump tube, the four-way connector and the waste liquid line respectively.

[0027] Preferably, the nanomedicine preparation component further includes a first housing, with a touch screen disposed above the front end face of the first housing. A USB interface, a start / stop button, an emergency stop button, and a signal indicator light are respectively disposed on one side of the touch screen, and a barcode scanner is disposed on the other side of the touch screen.

[0028] The first housing has a control system circuit board inside. The USB interface, the start / stop button, the emergency stop button, the signal indicator light, the touch screen, the barcode scanner, and the peristaltic pump, centrifugal pump, check valve, pinch valve, flow sensor, and mass flow meter installed on the pipeline are all electrically connected to the control system circuit board.

[0029] Preferably, the first housing is provided with a leakage collection tray and a second leakage detection sensor for detecting leakage in the pipeline.

[0030] The beneficial effects of this invention are: this nanomedicine preparation device has the advantages of convenient operation, compact structure, advanced and simple process, and precise and stable operation.

[0031] By loading liquid through the buffer cart assembly, the pressure can be kept constant when the liquid enters the pump head, reducing liquid disturbance. At the same time, it avoids the liquid level height of the external liquid dispensing equipment from affecting the flow rate accuracy of this device, thereby improving the stability of the nano-preparation system and enhancing its compatibility in different working environments. By using the buffer cart assembly to pre-set the buffer volume, the stability of the preparation process can be guaranteed regardless of the preparation volume.

[0032] Furthermore, by calibrating the first, second, and third ultrasonic flow sensors using mass flow meters, the accuracy of flow rates at each stage of nanomedicine preparation was ensured, thereby improving the stability of the large-scale production process. Simultaneously, by implementing a cleaning process for the mass flow meters, their accuracy can be maintained more effectively, thus guaranteeing their performance.

[0033] Furthermore, the use of standard solution prefilling in the prefilling stage effectively reduces lipid loss, thereby lowering the cost of large-scale nanomedicine preparation.

[0034] Based on this, by optimizing the flow path of consumables in the nanomedicine preparation components, the use of disposable consumable kits not only makes operation convenient but also effectively reduces liquid residue, further lowering production costs. Attached Figure Description

[0035] Figure 1 This is an overall schematic diagram of the nanomedicine preparation device of this utility model;

[0036] Figure 2 This is a front view of the nanomedicine preparation device of this utility model;

[0037] Figure 3 This is a schematic diagram of the nanomedicine preparation component of this utility model;

[0038] Figure 4 This is another schematic diagram of the nanomedicine preparation component of this utility model;

[0039] Figure 5 This is a front view of the buffer vehicle assembly of this utility model;

[0040] Figure 6 This is an isometric view of the buffer vehicle assembly of this utility model;

[0041] Figure 7 This is a detailed diagram of the piping connections of the nanomedicine preparation device of this utility model;

[0042] Figure 8 This is a structural schematic diagram of the disposable consumable kit of this utility model;

[0043] Figure 9 This is a side view of the disposable consumable kit of this utility model;

[0044] Figure 10 This is a schematic diagram of the preparation method of the nanomedicine preparation device of this utility model;

[0045] Figure 11 This is a detailed diagram of step S1 of the preparation method of the nanomedicine preparation device of this utility model;

[0046] Figure 12 This is a detailed diagram of step S2 of the preparation method of the nanomedicine preparation device of this utility model;

[0047] Figure 13 This is a detailed diagram of step S3 in the preparation method of the nanomedicine preparation device of this utility model;

[0048] Figure 14 This is a detailed diagram of step S4 in the preparation method of the nanomedicine preparation device of this utility model;

[0049] Figure 15 This is a detailed diagram of step S5 in the preparation method of the nanomedicine preparation device of this utility model;

[0050] Figure 16 This is a detailed diagram of step S6 in the preparation method of the nanomedicine preparation device of this utility model.

[0051] In the diagram: 1. Nanomedicine preparation component; 2. Buffer cart component; 201. Third shell; 3. Lipid phase storage component; 301. Sixth shell; 4. Aqueous phase storage component; 401. Fourth shell; 5. Dilution phase storage component; 501. Fifth shell; 6. Harvest phase storage component; 601. Second shell; 7. First shell; 8. USB interface; 9. Start / Stop button; 10. Emergency stop button; 12. Signal indicator light; 13. Touch screen; 14. Barcode scanner; 48. Standard solution storage bag; 49. First standard solution connection line; 50. Second standard solution connection line; 51. Third standard solution connection line. 52. First pipe clamp; 53. Second pipe clamp; 54. Third pipe clamp; 54. First pump connector connecting pipe; 55. Second pump connector connecting pipe; 56. Third pump connector connecting pipe; 57. First centrifugal pump; 58. Second centrifugal pump; 59. Third centrifugal pump; 60. First ultrasonic flow sensor; 61. Second ultrasonic flow sensor; 62. Third ultrasonic flow sensor; 63. First clamp valve; 64. Second clamp valve; 65. Third clamp valve; 66. First check valve; 67. Second check valve; 68. Third check valve; 69. Fourth check valve; 70. Fifth check valve; 71. 72. Sixth check valve; 73. First pipeline; 74. Fourth clamp valve; 75. Sixth pipeline; 76. Fourth clamp; 77. Finished product collection box; 78. Fifth clamp valve; 79. Fourth peristaltic pump; 80. Peristaltic pump tubing; 81. Second pipeline; 82. Third pipeline; 83. Mass flow meter; 84. Fourth pipeline; 85. Pneumatic ball valve; 86. Fifth pipeline; 87. Waste liquid collection box; 88. First leak detection sensor; 89. Second leak detection sensor; 90. First buffer cart connection pipeline; 91. Second buffer cart connection pipeline; 92. Third buffer cart connection pipeline; 93. Fifth clamp; 94. 3. Sixth clamp; 94. Seventh clamp; 95. Dilute phase buffer bag; 96. Aqueous phase buffer bag; 97. Lipid phase buffer bag; 98. First weighing sensor; 99. Second weighing sensor; 100. Third weighing sensor; 101. First peristaltic pump; 102. Second peristaltic pump; 103. Third peristaltic pump; 104. Lipid phase storage bag; 105. Aqueous phase storage bag; 106. Dilute phase storage bag; 107. Dilute phase delivery line; 108. Aqueous phase delivery line; 109. Lipid phase delivery line; 110. Microfluidic chip; 111. First injection line; 112. Second injection line. Detailed Implementation

[0052] The technical solution of this utility model will now be clearly and completely described in conjunction with the accompanying drawings. In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description. They do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0053] The present invention will be further described below with reference to the accompanying drawings.

[0054] like Figure 1-7 As shown, a nanomedicine preparation device includes: a nanomedicine preparation component 1, a buffer cart component 2, a lipid phase storage component 3, an aqueous phase storage component 4, a dilution phase storage component 5, a harvest phase storage component 6, and a liquid circuit component; the buffer cart component 2 has a lipid phase buffer unit, an aqueous phase buffer unit, and a dilution phase buffer unit; the output ends of the lipid phase storage component 3, the aqueous phase storage component 4, and the dilution phase storage component 5 are connected one-to-one with the input ends of a plurality of the lipid phase buffer units, the aqueous phase buffer units, and the dilution phase buffer units; the output ends of the lipid phase buffer units, the aqueous phase buffer units, and the dilution phase buffer units are connected one-to-one with the input ends of the nanomedicine preparation component 1, and the output end of the nanomedicine preparation component 1 is connected to the input end of the harvest phase storage component 6; the liquid circuit component is equipped with a first peristaltic pump 101 and a second peristaltic pump 102. 2. A third peristaltic pump 103, a first centrifugal pump 57, a second centrifugal pump 58, and a third centrifugal pump 59; the first peristaltic pump 101 is used to transport the lipid phase stored in the lipid phase storage component 3 to the lipid phase buffer unit, and the first centrifugal pump 57 is used to transport the lipid phase in the lipid phase buffer unit to the nanomedicine preparation component 1; the second peristaltic pump 102 is used to transport the aqueous phase stored in the aqueous phase storage component 4 to the aqueous phase buffer unit, and the second centrifugal pump 58 is used to transport the aqueous phase in the aqueous phase buffer unit to the nanomedicine preparation component 1; the third peristaltic pump 103 is used to transport the dilution phase stored in the dilution phase storage component 5 to the dilution phase buffer unit, and the third centrifugal pump 59 is used to transport the dilution phase in the dilution phase buffer unit to the nanomedicine preparation component 1.

[0055] The nanomedicine preparation component 1 includes a microfluidic chip 110, a first injection line 111, a second injection line 112, a first line 72, a sixth line 74, a fourth peristaltic pump 78, and a fluid control unit. A first centrifugal pump 57 is connected to the first inlet end of the microfluidic chip 110 through the first injection line 111, a second centrifugal pump 58 is connected to the second inlet end of the microfluidic chip 110 through the second injection line 112, and a four-way connector is provided at the outlet end of the microfluidic chip 110. The first end of the four-way connector is connected to the outlet end of the microfluidic chip 110. A third centrifugal pump 59 is connected to the second end of the four-way connector through the first line 72, and the fourth peristaltic pump 78 is connected to the third end of the four-way connector through the peristaltic pump tube 79. The fourth end of the four-way connector is connected to the sixth line 74. The fluid control unit includes a one-way valve, a clamp valve, and a flow sensor disposed on the first injection line 111, the second injection line 112, and the first line 72.

[0056] Specifically, the microfluidic chip 110 has two inlet ends and one outlet end. One inlet end is connected to the first sample injection line 111, and the other inlet end is connected to the second sample injection line 112. The first line 72 is connected to the outlet end of the microfluidic chip 110 through a four-way connector.

[0057] The first sample inlet line 111, the second sample inlet line 112, and the first line 72 are all equipped with a check valve, a pinch valve, and a flow sensor to control the flow of fluid.

[0058] like Figure 7 As shown, the first sample inlet line 111 is equipped with a fourth check valve 69, a first check valve 66, a first pinch valve 63, and a first ultrasonic flow sensor 60 from top to bottom; the second sample inlet line 112 is equipped with a fifth check valve 70, a second check valve 67, a second pinch valve 64, and a second ultrasonic flow sensor 61 from top to bottom; and the first line 72 is equipped with a sixth check valve 71, a third check valve 68, a third pinch valve 65, and a third ultrasonic flow sensor 62 from top to bottom. In this embodiment, the first centrifugal pump 57, the second centrifugal pump 58, and the third centrifugal pump 59 are all magnetic levitation pumps. Due to the characteristics of magnetic levitation pumps, there is a situation of low pressure and unstable flow rate. Therefore, two check valves are provided on the first sample inlet line 111, the second sample inlet line 112, and the first line 72. By increasing the hydraulic pressure through the two check valves, the flow rate uniformity can be controlled within 2%.

[0059] In this embodiment, the flow sensors installed on the first sample inlet line 111, the second sample inlet line 112, and the first line 72 are all ultrasonic flow sensors. The ultrasonic flow sensors are used not only to detect the flow rate uniformity, but also to detect bubbles.

[0060] like Figure 7 As shown, the pump head of the first centrifugal pump 57 is connected to the first ultrasonic flow sensor 60, the pump head of the second centrifugal pump 58 is connected to the second ultrasonic flow sensor 61, and the pump head of the third centrifugal pump 59 is connected to the third ultrasonic flow sensor 62. The first ultrasonic flow sensor 60, the second ultrasonic flow sensor 61, and the third ultrasonic flow sensor 62 can all detect bubbles in their respective pump heads.

[0061] Please continue to refer to this. Figure 1-2 5-6, the buffer vehicle assembly 2 has several buffer units (i.e., lipid phase buffer unit, aqueous phase buffer unit and dilution phase buffer unit), each buffer unit has an input end and an output end, the input ends of the several buffer units are connected one-to-one with the output ends of the lipid phase storage assembly 3, the aqueous phase storage assembly 4 and the dilution phase storage assembly 5, the output ends of the several buffer units are connected one-to-one with the input ends of the nano-drug preparation assembly 1, and the output end of the nano-drug preparation assembly 1 is connected with the input end of the harvest phase storage assembly 6.

[0062] The fluids provided by the lipid phase storage component 3, the aqueous phase storage component 4, and the dilution phase storage component 5 are all transported to the nanomedicine preparation component 1 through the buffer unit corresponding to the buffer carriage component 2. The nanomedicine preparation component 1 is used to receive and mix the fluids to generate nanoparticles. The harvest phase storage component 6 is connected to the nanomedicine preparation component 1 and is used to receive and store the nanoparticles. The buffer carriage component 2 allows the nanomedicine preparation device to be compatible with various preparation volumes. Regardless of the preparation volume, the buffer volume can be preset to ensure the stability of nanomedicine preparation.

[0063] Please continue to refer to this. Figure 7 The nanomedicine preparation device further includes: a standard solution storage container 48 and a standard solution pipeline. The standard solution pipeline includes a first standard solution connecting pipeline 49, a second standard solution connecting pipeline 50, and a third standard solution connecting pipeline 51. One end of each of the first standard solution connecting pipeline 49, the second standard solution connecting pipeline 50, and the third standard solution connecting pipeline 51 is connected to the outlet of the standard solution storage container 48. The other end of the first standard solution connecting pipeline 49 is connected to a third centrifugal pump 59 through a third pump connector connecting pipe 56. The other end of the second standard solution connecting pipeline 50 is connected to a second centrifugal pump 58 through a second pump connector connecting pipe 55. The other end of the third standard solution connecting pipeline 51 is connected to a first centrifugal pump 57 through a first pump connector connecting pipe 54.

[0064] A first clamp 52 is provided on the third standard liquid connection pipe 51, a second clamp 53 is provided on the second standard liquid connection pipe 50, and a third clamp 541 is provided on the first standard liquid connection pipe 49.

[0065] Please continue to refer to this. Figure 7 The nanomedicine preparation device further includes a waste liquid collection container 86 and a waste liquid pipeline. The waste liquid pipeline includes a second pipeline 80, a third pipeline 81, a fourth pipeline 83, and a fifth pipeline 85. The fourth pipeline 83 and the fifth pipeline 85 are connected in parallel between the waste liquid collection container 86 and the third pipeline 81. The second pipeline 80 and the fourth peristaltic pump 78 are connected in parallel between the third pipeline 81 and the third end of the four-way connector. A fifth clamp valve 77 is provided on the second pipeline 80. A mass flow meter 82 is provided on the fourth pipeline 83. A pneumatic ball valve 84 is provided on the fifth pipeline 85. One end of the second pipeline 80 and the peristaltic pump pipe 79 are connected to the third end of the four-way connector. The other end of the second pipeline 80 and the fourth peristaltic pump 78 are connected to the third pipeline 81. The other ends of the fourth pipeline 83 and the fifth pipeline 85 are connected to the waste liquid collection box 86.

[0066] When the pneumatic ball valve 84 is opened, the waste liquid pipeline is connected; when the pneumatic ball valve 84 is closed, the accuracy of the ultrasonic flow sensor is calibrated by the mass flow meter 82.

[0067] Preferably, a first leakage detection sensor 87 is provided on both the fourth pipeline 83 and the fifth pipeline 85, and the first leakage detection sensor 87 is integrated with the mass flow meter 82.

[0068] Please continue to refer to this. Figure 1-4 The nanomedicine preparation component 1 also includes a first housing 7. A touch screen 13 is provided on the upper front surface of the first housing 7. A USB interface 8, a start / stop button 9, an emergency stop button 10, and a signal indicator light 12 are respectively provided on one side of the touch screen 13. A barcode scanner 14 is provided on the other side of the touch screen 13. A control system circuit board is provided inside the first housing 7. The USB interface 8, start / stop button 9, emergency stop button 10, signal indicator light 12, touch screen 13, barcode scanner 14, and peristaltic pumps (i.e., first peristaltic pump 101, second peristaltic pump 102, third peristaltic pump 103, and fourth peristaltic pump 78), centrifugal pumps (i.e., first centrifugal pump 57, second centrifugal pump 58, and third centrifugal pump 59), check valves, pinch valves, flow sensors, and mass flow meters provided on the pipeline are all electrically connected to the control system circuit board.

[0069] In this embodiment, the electrical components of the nanomedicine preparation component 1, the buffer vehicle component 2, the lipid phase storage component 3, the aqueous phase storage component 4, the dilution phase storage component 5, and the harvest phase storage component 6 are all electrically connected to the control system circuit board to achieve unified control of each component.

[0070] Please continue to refer to this. Figure 1 and Figure 7The harvest phase storage component 6 includes a second housing 601, a finished product collection container 76 is disposed inside the second housing 601, the inlet of the finished product collection container 76 is connected to a sixth pipe 74, and a fourth clamp valve 73 and a fourth pipe clamp 75 are disposed on the sixth pipe 74.

[0071] Please continue to refer to this. Figure 5-6 The buffer vehicle assembly 2 includes a third housing 201. A first weighing sensor 98, a second weighing sensor 99, and a third weighing sensor 100 are respectively installed on the top of the third housing 201. The lower end of the first weighing sensor 98 is connected to a dilution phase buffer bag 95, the lower end of the second weighing sensor 99 is connected to an aqueous phase buffer bag 96, and the lower end of the third weighing sensor 100 is connected to a lipid phase buffer bag 97. The dilution phase buffer bag 95, the aqueous phase buffer bag 96, and the lipid phase buffer bag 97 are all suspended on the hooks of their corresponding weighing sensors.

[0072] Please continue to refer to this. Figure 5-7 The first peristaltic pump 101, the second peristaltic pump 102, and the third peristaltic pump 103 are all disposed on the outer wall of the third housing 201. The first peristaltic pump 101 is connected to the lipid phase delivery pipeline 109 and is used to deliver liquid from the lipid phase storage component 3 to the lipid phase buffer bag 97. The second peristaltic pump 102 is connected to the aqueous phase delivery pipeline 108 and is used to deliver liquid from the aqueous phase storage component 4 to the aqueous phase buffer bag 96. The third peristaltic pump 103 is connected to the dilution phase delivery pipeline 10... 7 is a connection used to transport the liquid in the dilution phase storage assembly 5 to the dilution phase buffer bag 95; the outlet of the dilution phase buffer bag 95 is connected to the third centrifugal pump 59 via the third buffer cart connection pipe 91 and the third pump connector connection pipe 56; the outlet of the aqueous phase buffer bag 96 is connected to the second centrifugal pump 58 via the second buffer cart connection pipe 90 and the second pump connector connection pipe 55; the outlet of the lipid phase buffer bag 97 is connected to the first centrifugal pump 57 via the first buffer cart connection pipe 89 and the first pump connector connection pipe 54.

[0073] The third buffer car connecting pipe 91 is connected to the seventh clamp 94; the second buffer car connecting pipe 90 is connected to the sixth clamp 93; and the first buffer car connecting pipe 89 is connected to the fifth clamp 92.

[0074] In this embodiment, both the pipe clamp and the clamp valve are used to control the on / off state of the pipeline. The pipe clamp is manually operated, while the clamp valve is electrically controlled.

[0075] Preferably, the outlets of the dilution phase buffer bag 95, the aqueous phase buffer bag 96, and the lipid phase buffer bag 97 are all located at the bottom of the bag. The first clamp 52, the second clamp 53, the third clamp 541, the fourth clamp 75, the fifth clamp 92, the sixth clamp 93, and the seventh clamp 94 are all Robert clamps.

[0076] Please continue to refer to this. Figure 1 and Figure 7 The lipid phase storage assembly 3 includes a sixth housing 301, within which a lipid phase storage bag 104 is disposed, and the outlet of the lipid phase storage bag 104 is connected to a first peristaltic pump 101; the aqueous phase storage assembly 4 includes a fourth housing 401, within which an aqueous phase storage bag 105 is disposed, and the outlet of the aqueous phase storage bag 105 is connected to a second peristaltic pump 102; the dilution phase storage assembly 5 includes a fifth housing 501, within which a dilution phase storage bag 106 is disposed, and the outlet of the dilution phase storage bag 106 is connected to a third peristaltic pump 103.

[0077] Please refer to Figure 8 and Figure 9 The pump heads 38 of the first centrifugal pump 57, the second centrifugal pump 58, and the third centrifugal pump 59, together with the sterile connector 39, the clamp 40, the first pipeline 72, the microfluidic chip 110, the four-way connector 44, the three-way connector 45, and the peristaltic pump tube 79, form a disposable consumable kit. Among them, the first pipeline 72 is a silicone tube, and the inlet of the flow sensor (i.e., the first ultrasonic flow sensor 60, the second ultrasonic flow sensor 61, and the third ultrasonic flow sensor 62) is connected to the outlet of the pump head 38 of the three centrifugal pumps one by one, and is fixed together by the clamp 40. The three-way connector 45 is used to connect the peristaltic pump tube 79, the four-way connector 44, and the waste liquid pipeline respectively.

[0078] Please continue to refer to this. Figure 1 and Figure 7 The first housing 7 is provided with a leakage collection tray 19 and a second leakage detection sensor 88 for detecting leakage in the pipeline. The second leakage detection sensor 88 is located at the leakage collection tray 19 and detects the liquid in the leakage collection tray 19 by laser reflection.

[0079] Preferably, the first leakage detection sensor 87 and the second leakage detection sensor 88 are both disposed inside the first housing 7.

[0080] like Figure 10-16 As shown, a preparation method based on a nanomedicine preparation device includes:

[0081] Step S1: Loading the buffer cart with liquid bags;

[0082] Step S2, pre-filling;

[0083] Step S3, flow calibration;

[0084] Step S4: Discharge of waste liquid from the pre-treatment stage;

[0085] Step S5, preparation;

[0086] Step S6: Clean the mass flow meter.

[0087] Specifically, step S1, the process of loading the buffer tank liquid bag, includes:

[0088] Step S11, lipid phase liquid bag loading: The control system circuit board sends a lipid phase loading command, the first peristaltic pump 101 on the buffer cart works, and the liquid in the lipid phase storage bag 104 is transported to the lipid phase buffer bag 97; when the third weighing sensor 100 detects that the liquid in the lipid phase buffer bag 97 has reached the program set weight, the first peristaltic pump 101 stops working, and the loading is completed.

[0089] Step S12, Aqueous phase liquid bag loading: The control system circuit board issues an aqueous phase loading command, and the second peristaltic pump 102 on the buffer cart works to transport the liquid in the aqueous phase storage bag 105 to the aqueous phase buffer bag 96; when the second weighing sensor 99 detects that the liquid in the aqueous phase buffer bag 96 has reached the program-set weight, the second peristaltic pump 102 stops working, and the loading is completed.

[0090] Step S13, dilution phase liquid bag loading: The control system circuit board issues a dilution phase loading command, and the third peristaltic pump 103 on the buffer cart works to transport the liquid in the dilution phase storage bag 106 to the dilution phase buffer bag 95; when the first weighing sensor 98 detects that the liquid in the dilution phase buffer bag 95 has reached the program-set weight, the third peristaltic pump 103 stops working, and the loading is completed.

[0091] Step S2, the pre-filling process includes:

[0092] Step S21, Lipid Phase Pre-filling: Open the first clamp 52, and the standard solution storage bag 48 is connected to the first pump connector connecting pipe 54 through the third standard solution connecting pipe 51; the control system circuit board issues a lipid phase pre-filling command, opening the fourth peristaltic pump 78, the pneumatic ball valve 84, and the clamp valve on the lipid phase pre-filling pipe. Under the action of the fourth peristaltic pump 78, the standard solution flows from the standard solution storage bag 48 through the first centrifugal pump 57, the first ultrasonic flow sensor 60, and the fourth one-way valve 69, entering the microfluidic chip 110; from the microfluidic... After the chip 110 exits, the liquid flows through the peristaltic pump tube 79 and the fifth tube 85 into the waste liquid collection box 86. During the pre-filling process, the standard liquid enters the centrifugal pump head and discharges air bubbles from the pump head. When the first ultrasonic flow sensor 60 detects the liquid flowing through within a set time (according to the algorithm, the set time is considered to have been reached when the sensor data reaches the set range), and detects no air bubbles inside the tube, the lipid phase pre-filling ends. At this time, the corresponding clamp valve is closed, and the first tube clamp 52 is manually closed to prevent liquid backflow.

[0093] Step S22, Aqueous Phase Pre-filling: Open the second clamp 53, and the standard solution storage bag 48 is connected to the second pump connector connecting pipe 55 through the second standard solution connecting pipe 50; the control system circuit board issues an aqueous phase pre-filling command, opening the fourth peristaltic pump 78, the pneumatic ball valve 84, and the clamp valve on the aqueous phase pre-filling pipe. Under the action of the fourth peristaltic pump 78, the standard solution flows from the standard solution storage bag 48 through the second centrifugal pump 58, the second ultrasonic flow sensor 61, and the second one-way valve 67, entering the microfluidic chip 110; from the microfluidic chip... After flowing out of the outlet of 110, it enters the waste liquid collection box 86 via the peristaltic pump pipe 79 and the fifth pipe 85; similarly, during the pre-filling process, the standard liquid enters the centrifugal pump head and discharges the air bubbles in the pump head; when the second ultrasonic flow sensor 61 detects the liquid flowing through within the set time (according to the algorithm, the set time is considered to have been reached when the sensor data reaches the set range), and there are no air bubbles inside the liquid, the aqueous phase pre-filling ends; at this time, the corresponding clamp valve is closed, and the second pipe clamp 53 is manually closed to prevent liquid backflow;

[0094] Step S23, dilution phase pre-filling: Open the third clamp 541, and the standard solution storage bag 48 is connected to the third pump connector connecting pipe 56 through the first standard solution connecting pipe 49; the control system circuit board issues a dilution phase pre-filling command, opening the fourth peristaltic pump 78, the pneumatic ball valve 84, and the clamp valve on the dilution phase pre-filling pipe. Under the action of the fourth peristaltic pump 78, the standard solution flows from the standard solution storage bag 48 through the third centrifugal pump 59, the third ultrasonic flow sensor 62, and the sixth one-way valve 71 into the peristaltic pump pipe 79. After flowing out of the peristaltic pump pipe 79, it enters the waste liquid collection box 86 through the fifth pipe 85; similarly, during the pre-filling process, the standard solution enters the centrifugal pump head and discharges air bubbles from the pump head; when the third ultrasonic flow sensor 62 detects liquid flow within a set time (according to the algorithm, if the sensor data reaches the set range, it is considered that the set time has been reached), and there are no air bubbles inside the liquid, the dilution phase pre-filling ends; at this time, the corresponding clamp valve is closed to prevent liquid backflow.

[0095] Step S3, the flow calibration process includes:

[0096] Step S31, Lipid Phase Calibration: Open the first clamp 52, and connect the standard solution storage bag 48 to the first pump connector connecting pipe 54 through the third standard solution connecting pipe 51; the control system circuit board issues a lipid phase calibration command, turns on the first centrifugal pump 57, and under the action of the first centrifugal pump 57, the standard solution flows from the standard solution storage bag 48 through the pump head of the first centrifugal pump 57, the first ultrasonic flow sensor 60 and the fourth one-way valve 69, and enters the microfluidic chip 110; the standard solution flows out from the outlet of the microfluidic chip 110, through the second pipe 80, the third pipe 81, the fourth pipe 83 and the mass flow meter 82, and enters the waste liquid collection box 86; the standard solution flow rate detected by the mass flow meter 82 and the flow rate detected by the first ultrasonic flow sensor 60 are calculated by the program algorithm to obtain a compensation difference value, and this compensation difference value is transmitted to the first ultrasonic flow sensor 60 to calibrate the data of the first ultrasonic flow sensor 60; after the lipid phase calibration is completed, close the corresponding clamp valve and manually close the first clamp 52 to prevent liquid backflow;

[0097] Step S32, Aqueous Phase Calibration: Open the second clamp 53. The standard solution storage bag 48 is connected to the control system circuit board via the second standard solution connecting pipe 50 and the second pump connector connecting pipe 55 to issue an aqueous phase calibration command. Turn on the second centrifugal pump 58. Under the action of the second centrifugal pump 58, the standard solution flows from the standard solution storage bag 48 through the pump head of the second centrifugal pump 58, the second ultrasonic flow sensor 61, and the fifth one-way valve 70, and enters the microfluidic chip 110. After flowing out of the outlet of the microfluidic chip 110, it flows through the second pipe 80, the third pipe 81, the fourth pipe 83, and enters the mass flow meter 82, and then enters the waste liquid collection box 86. The standard solution flow rate detected by the mass flow meter 82 and the flow rate detected by the second ultrasonic flow sensor 61 are calculated by the program algorithm to obtain a compensation difference value. This compensation difference value is transmitted to the second ultrasonic flow sensor 61 to calibrate the data of the second ultrasonic flow sensor 61. When the aqueous phase calibration is completed, close the corresponding clamp valve and manually close the second clamp 53 to prevent liquid backflow.

[0098] Step S33, dilution phase calibration: Open the third clamp 541, and connect the standard solution storage bag 48 to the third pump connector connecting pipe 56 through the first standard solution connecting pipe 49; the control system circuit board issues a dilution phase calibration command, turns on the third centrifugal pump 59, and under the action of the third centrifugal pump 59, the standard solution flows from the standard solution storage bag 48 through the pump head of the third centrifugal pump 59, the third ultrasonic flow sensor 62, the sixth one-way valve 71, the second pipe 80, the third pipe 81, the fourth pipe 83, and the mass flow meter 82, and enters the waste liquid collection box 86; calculate the standard solution flow rate detected by the mass flow meter 82 and the flow rate detected by the third ultrasonic flow sensor using a program algorithm to obtain a compensation difference value, and transmit this compensation difference value to the third ultrasonic flow sensor 62 to calibrate the data of the third ultrasonic flow sensor 62; when the aqueous phase calibration is completed, close the corresponding clamp valve and manually close the third clamp 541 to prevent liquid backflow.

[0099] Step S4, the process of discharging the waste liquid from the pre-treatment stage includes:

[0100] Step S41: Close the first clamp 52, the second clamp 53, and the third clamp 541; first open the fifth clamp 92, the sixth clamp 93, and the seventh clamp 94; then open the first clamp valve 63, the second clamp valve 64, the third clamp valve 65, and the fifth clamp valve 77; open the pneumatic ball valve 84; at this time, open the first centrifugal pump 57, the second centrifugal pump 58, and the third centrifugal pump 59 to drive the liquid through the lipid phase, aqueous phase, and dilution phase pipelines. After converging at the outlet of the microfluidic chip 110, the liquid enters the waste liquid collection box 86 through the second pipeline 80, the third pipeline 81, and the fifth pipeline 85; when the flow rate of the pre-preparation waste liquid reaches the set value, the pre-preparation waste liquid discharge is completed.

[0101] Step S5, the preparation process includes:

[0102] Step S51: After the waste liquid from the pre-processing stage is discharged, the first centrifugal pump 57, the second centrifugal pump 58, and the third centrifugal pump 59 continue to operate; the fourth clamp 75 is opened, the fifth clamp valve is closed, the pneumatic ball valve 84 is closed, and the fourth clamp valve 73 is opened, so that the preparation liquid flowing out of the microfluidic chip 110 outlet enters the finished product collection box 76 through the sixth pipeline 74; the preparation is completed when the first weighing sensor 98, the second weighing sensor 99, and the third weighing sensor 100 of the buffer cart detect that the liquid in the buffer bag is used up; the first centrifugal pump 57, the second centrifugal pump 58, and the third centrifugal pump 59 are closed; the first clamp valve 63, the second clamp valve 64, the third clamp valve 65, and the fourth clamp valve 73 are closed.

[0103] Step S6, mass flow meter cleaning includes:

[0104] Step S61: After preparation is completed, open the third clamp 541 corresponding to the standard solution storage bag 48; open the third clamp valve 65 and the fifth clamp valve; start the third centrifugal pump 59 to allow the standard solution to enter the mass flow meter 82 from the dilution phase flow path for cleaning; when the cleaning time reaches the set time, the cleaning is completed.

[0105] This nanomedicine preparation device boasts advantages such as convenient operation, compact structure, advanced and simple process, and precise and stable operation. Liquid is loaded via the buffer carriage assembly 2, ensuring constant pressure as the liquid enters the pump head, reducing liquid disturbance, and preventing the liquid level of external dispensing equipment from affecting the flow rate accuracy of this device. This enhances the system's stability in nanomedicine preparation and strengthens its compatibility under different operating environments.

[0106] Furthermore, by calibrating the first ultrasonic flow sensor 60, the second ultrasonic flow sensor 61, and the third ultrasonic flow sensor 62 using a mass flow meter 82, the accuracy of flow rates at each stage of nanomedicine preparation is ensured, thereby improving the stability of the large-scale production process. Simultaneously, by setting up a cleaning process for the mass flow meter, its accuracy can be maintained more effectively, thus ensuring the performance of the mass flow meter.

[0107] Furthermore, by using a standard solution prefilling method in the prefilling stage, lipid loss is effectively reduced, thereby lowering the cost of large-scale nanomedicine preparation.

[0108] Furthermore, the nanomedicine preparation component 1 features an optimized consumable flow path design. The use of disposable consumable kits not only makes operation convenient but also effectively reduces liquid residue, thereby lowering production costs.

[0109] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this utility model.

Claims

1. A nanomedicine preparation device, characterized in that, include: Nanomedicine preparation component (1), buffer cart component (2), lipid phase storage component (3), aqueous phase storage component (4), dilution phase storage component (5), harvest phase storage component (6), and liquid circuit component; The buffer vehicle assembly (2) has a lipid phase buffer unit, an aqueous phase buffer unit, and a dilution phase buffer unit. The output ends of the lipid phase storage assembly (3), the aqueous phase storage assembly (4), and the dilution phase storage assembly (5) are connected to the input ends of the lipid phase buffer unit, the aqueous phase buffer unit, and the dilution phase buffer unit respectively. The output ends of the lipid phase buffer unit, the aqueous phase buffer unit, and the dilution phase buffer unit are connected to the input ends of the nanomedicine preparation assembly (1). The output end of the nanomedicine preparation assembly (1) is connected to the input end of the harvest phase storage assembly (6). The liquid circuit assembly is equipped with a first peristaltic pump (101), a second peristaltic pump (102), a third peristaltic pump (103), a first centrifugal pump (57), a second centrifugal pump (58), and a third centrifugal pump (59). The first peristaltic pump (101) is used to transport the lipid phase stored in the lipid phase storage assembly (3) to the lipid phase buffer unit, and the first centrifugal pump (57) is used to transport the lipid phase in the lipid phase buffer unit to the nanomedicine preparation assembly (1). The second peristaltic pump (102) is used to transport the aqueous phase stored in the aqueous phase storage assembly (4) to the aqueous phase buffer unit, and the second centrifugal pump (58) is used to transport the aqueous phase in the aqueous phase buffer unit to the nanomedicine preparation assembly (1). The third peristaltic pump (103) is used to transport the dilution phase stored in the dilution phase storage assembly (5) to the dilution phase buffer unit, and the third centrifugal pump (59) is used to transport the dilution phase in the dilution phase buffer unit to the nanomedicine preparation assembly (1).

2. The nanomedicine preparation apparatus according to claim 1, characterized in that, The nanomedicine preparation component (1) includes a microfluidic chip (110), a first injection line (111), a second injection line (112), a first line (72), a sixth line (74), a fourth peristaltic pump (78), and a fluid control unit; The first centrifugal pump (57) is connected to the first inlet end of the microfluidic chip (110) through the first sample inlet line (111), the second centrifugal pump (58) is connected to the second inlet end of the microfluidic chip (110) through the second sample inlet line (112), the outlet end of the microfluidic chip (110) is provided with a four-way connector, the first end of the four-way connector is connected to the outlet end of the microfluidic chip (110), the third centrifugal pump (59) is connected to the second end of the four-way connector through the first line (72), the fourth peristaltic pump (78) is connected to the third end of the four-way connector through the peristaltic pump tube (79), and the fourth end of the four-way connector is connected to the sixth line (74). The fluid control unit includes a check valve, a pinch valve, and a flow sensor disposed on the first injection line (111), the second injection line (112), and the first line (72).

3. The nanomedicine preparation apparatus according to claim 2, characterized in that, The first centrifugal pump (57), the second centrifugal pump (58), and the third centrifugal pump (59) are all magnetic levitation pumps. Two one-way valves are provided on the first sample inlet line (111), the second sample inlet line (112), and the first line (72).

4. The nanomedicine preparation apparatus according to claim 1, characterized in that, It also includes a standard solution storage container (48) and a standard solution pipeline, wherein the standard solution pipeline includes a first standard solution connection pipeline (49), a second standard solution connection pipeline (50) and a third standard solution connection pipeline (51); one end of the first standard solution connection pipeline (49), the second standard solution connection pipeline (50) and the third standard solution connection pipeline (51) are all connected to the outlet of the standard solution storage container (48); the other end of the first standard solution connection pipeline (49) is connected to the third centrifugal pump (59) through a third pump connector connection pipe (56); the other end of the second standard solution connection pipeline (50) is connected to the second centrifugal pump (58) through a second pump connector connection pipe (55); and the other end of the third standard solution connection pipeline (51) is connected to the first centrifugal pump (57) through a first pump connector connection pipe (54). Pipe clamps are provided on the third standard solution connecting pipe (51), the second standard solution connecting pipe (50), and the first standard solution connecting pipe (49).

5. The nanomedicine preparation apparatus according to claim 2, characterized in that, It also includes a waste liquid collection container (86) and a waste liquid pipeline, wherein the waste liquid pipeline includes a second pipeline (80), a third pipeline (81), a fourth pipeline (83) and a fifth pipeline (85), the fourth pipeline (83) and the fifth pipeline (85) are connected in parallel between the waste liquid collection container (86) and the third pipeline (81), and the second pipeline (80) and the fourth peristaltic pump (78) are connected in parallel between the third pipeline (81) and the third end of the four-way connector; A pinch valve is installed on the second pipeline (80), a mass flow meter (82) is installed on the fourth pipeline (83), and a pneumatic ball valve (84) is installed on the fifth pipeline (85).

6. The nanomedicine preparation apparatus according to claim 5, characterized in that, Both the fourth pipeline (83) and the fifth pipeline (85) are equipped with a first leakage detection sensor (87).

7. The nanomedicine preparation apparatus according to claim 2, characterized in that, The harvest phase storage assembly (6) includes a second housing (601), a finished product collection container (76) is provided inside the second housing (601), the inlet of the finished product collection container (76) is connected to the sixth pipeline (74), and the sixth pipeline (74) is provided with a clamp valve and a pipe clamp.

8. The nanomedicine preparation apparatus according to claim 1, characterized in that, The buffer cart assembly (2) includes a third housing (201). A first weighing sensor (98), a second weighing sensor (99), and a third weighing sensor (100) are respectively disposed on the top of the third housing (201). The lower end of the first weighing sensor (98) is connected to a dilution phase buffer bag (95), the lower end of the second weighing sensor (99) is connected to an aqueous phase buffer bag (96), and the lower end of the third weighing sensor (100) is connected to a lipid phase buffer bag (97). The first peristaltic pump (101), the second peristaltic pump (102), and the third peristaltic pump (103) are all disposed on the third housing. On the outer wall of the three-shell casing (201), the first peristaltic pump (101) is disposed on the lipid phase delivery pipeline (109) for delivering liquid in the lipid phase storage component (3) to the lipid phase buffer bag (97); the second peristaltic pump (102) is disposed on the aqueous phase delivery pipeline (108) for delivering liquid in the aqueous phase storage component (4) to the aqueous phase buffer bag (96); and the third peristaltic pump (103) is disposed on the dilution phase delivery pipeline (107) for delivering liquid in the dilution phase storage component (5) to the dilution phase buffer bag (95). The outlet of the dilution phase buffer bag (95) is connected to the third centrifugal pump (59) via the third buffer cart connecting pipe (91) and the third pump connector connecting pipe (56); the outlet of the aqueous phase buffer bag (96) is connected to the second centrifugal pump (58) via the second buffer cart connecting pipe (90) and the second pump connector connecting pipe (55); the outlet of the lipid phase buffer bag (97) is connected to the first centrifugal pump (57) via the first buffer cart connecting pipe (89) and the first pump connector connecting pipe (54). Pipe clamps are provided on the third buffer vehicle connecting pipe (91), the second buffer vehicle connecting pipe (90), and the first buffer vehicle connecting pipe (89).

9. The nanomedicine preparation apparatus according to claim 1, characterized in that, The lipid phase storage assembly (3) includes a sixth housing (301), a lipid phase storage bag (104) is disposed inside the sixth housing (301), and the outlet of the lipid phase storage bag (104) is connected to the first peristaltic pump (101); The aqueous phase storage assembly (4) includes a fourth housing (401), an aqueous phase storage bag (105) is disposed inside the fourth housing (401), and the outlet of the aqueous phase storage bag (105) is connected to the second peristaltic pump (102); The dilution phase storage assembly (5) includes a fifth housing (501), a dilution phase storage bag (106) is disposed inside the fifth housing (501), and the outlet of the dilution phase storage bag (106) is connected to the third peristaltic pump (103).

10. The nanomedicine preparation apparatus according to claim 2, characterized in that, The pump heads (38) of the first centrifugal pump (57), the second centrifugal pump (58) and the third centrifugal pump (59), together with the sterile connector (39), clamp (40), flow sensor, first pipeline (72), microfluidic chip (110), four-way connector (44), three-way connector (45), and peristaltic pump tubing (79), form a disposable consumable kit; The inlet of the flow sensor and the outlet of the pump head (38) are fixed together by the clamp (40), and the three-way connector (45) is used to connect the peristaltic pump pipe (79), the four-way connector (44) and the waste liquid pipeline respectively.

11. The nanomedicine preparation apparatus according to any one of claims 1 to 10, characterized in that, The nanomedicine preparation component (1) also includes a first housing (7), a touch screen (13) is provided above the front end face of the first housing (7), a USB interface (8), a start / stop button (9), an emergency stop button (10) and a signal indicator (12) are respectively provided on one side of the touch screen (13), and a barcode scanner (14) is provided on the other side of the touch screen (13). The first housing (7) is provided with a control system circuit board. The USB interface (8), the start / stop button (9), the emergency stop button (10), the signal indicator (12), the touch screen (13), the barcode scanner (14), and the peristaltic pump, centrifugal pump, check valve, clamp valve, flow sensor and mass flow meter installed on the pipeline are all electrically connected to the control system circuit board.

12. The nanomedicine preparation apparatus according to claim 11, characterized in that, The first housing (7) is provided with a leakage collection tray (19) and a second leakage detection sensor (88) for detecting pipeline leakage.

Images