Immobilized production system of chondrosulphatase

Abstract

The utility model provides an immobilization production system of chondroitin sulfate enzyme, belongs to the technical field of chondroitin sulfate production, and aims to solve the problems that in the immobilization process of the existing chondroitin sulfate enzyme, animal tissues need to be crushed by a homogenizer and then are mixed with an extraction buffer solution, and crude enzyme liquid is prepared after being preliminarily filtered by a filter screen, so that the production cost is low. However, when large-scale preparation is carried out, the filter screen is simple in structure and needs to be frequently replaced and maintained, the operation is too tedious, and the preparation efficiency is influenced. Comprising a chondroitin sulfate curing system; the carrier pretreatment device is used for treating carrier raw materials; the enzyme liquid preparation device is used for preparing an enzyme liquid; the constant flow pump is used for extracting the prepared enzyme liquid; and the immobilized reaction device is used for reacting the enzyme liquid with the carrier to prepare a target product. The device has the advantages of efficient immobilized production, blockage prevention, convenience in maintenance and the like.

Description

Technical Field

[0001] This invention belongs to the field of chondroitin sulfate production technology, and more specifically, relates to an immobilized production system for chondroitin sulfate enzyme. Background Technology

[0002] Chondroitin sulfate enzyme requires immobilization during processing and production. Existing chondroitin sulfate enzyme immobilization processes involve treating the carrier material using a carrier pretreatment device, followed by enzyme solution delivery and mixing, constant flow pump flow rate control, and a stirrer or static mixer to promote cross-linking, ensuring precise anchoring of the enzyme onto the carrier and achieving high-quality immobilization.

[0003] Based on the above, most existing chondroitin sulfate enzymes use microorganisms or animal tissues as raw materials during the immobilization process. When using animal tissues as raw materials, a homogenizer is needed to break down the animal tissues and mix them with the extraction buffer. After preliminary filtration through a filter screen, a crude enzyme solution is prepared. However, when preparing in large batches, the filter screen has a simple structure and requires frequent replacement and maintenance, making the operation too cumbersome and affecting the preparation efficiency. Utility Model Content

[0004] To address the aforementioned technical problems, this invention provides an immobilization production system for chondroitin sulfate enzyme. This system solves the problem that existing chondroitin sulfate enzyme immobilization processes mostly use microorganisms or animal tissues as raw materials. When using animal tissues as raw materials, a homogenizer is needed to break down the animal tissues before mixing them with an extraction buffer. After preliminary filtration through a filter screen, a crude enzyme solution is produced. However, for large-scale production, the simple filter screen structure requires frequent replacement and maintenance, making the operation overly cumbersome and affecting production efficiency.

[0005] The purpose and effectiveness of this novel chondroitin sulfate immobilization production system are achieved through the following specific technical means:

[0006] An immobilized production system for chondroitin sulfate enzyme, including a chondroitin sulfate immobilization system;

[0007] A carrier pretreatment device for processing carrier raw materials;

[0008] An enzyme solution preparation apparatus for preparing enzyme solutions;

[0009] A constant flow pump is used to extract the prepared enzyme solution.

[0010] An immobilization reaction apparatus, wherein the enzyme solution and the carrier are reacted to produce the target product.

[0011] Furthermore, the carrier pretreatment device includes:

[0012] An ultrasonic cleaner, wherein the ultrasonic cleaner performs ultrasonic cleaning on carrier materials;

[0013] A chemical reaction vessel is used to mix the cleaned carrier raw material with an activator under constant temperature and pressure conditions to produce an activated carrier.

[0014] Furthermore, the enzyme solution preparation device includes:

[0015] A tissue homogenizer, wherein the tissue homogenizer uses internal mechanical blades to break up animal tissue, causing intracellular chondroitin sulfate enzymes to be released into a buffer solution;

[0016] A preliminary filter is used to initially filter the broken animal tissue to obtain a crude enzyme solution.

[0017] Centrifuge, wherein the crude enzyme solution is centrifuged to separate the crude enzyme solution, so that most of the solid impurities precipitate to the bottom of the centrifuge tube, while the supernatant containing chondroitin sulfate is collected for subsequent purification.

[0018] An ion exchange chromatography column is used to separate and purify the supernatant after centrifugation to obtain a purified enzyme solution with a certain concentration.

[0019] Furthermore, the immobilization reaction apparatus includes:

[0020] A static mixer, wherein the enzyme solution and the carrier pretreatment solution are thoroughly reacted and mixed;

[0021] A fixed-bed reactor is provided, in which a carrier is installed inside. The enzyme solution flows through the carrier inside the fixed-bed reactor at a certain flow rate to fully react and generate the target product.

[0022] Furthermore, the preliminary filter includes:

[0023] A delivery pipeline is installed between the primary filter and the tissue homogenizer;

[0024] A rotating shaft is rotatably connected inside the conveying pipeline;

[0025] The water wheel is fixedly installed in the middle of the rotating shaft;

[0026] A cutter is fixedly mounted at the bottom of the rotating shaft.

[0027] Furthermore, the preliminary filter also includes:

[0028] Mounting plate, which is slidably connected inside the primary filter;

[0029] The first filter plate is snapped into the mounting plate;

[0030] The second filter plate is snapped into the mounting plate;

[0031] A drive motor, which is fixedly mounted on one side of the primary filter;

[0032] A threaded screw is rotatably connected inside the primary filter and fixedly installed at one end of the drive motor. The threaded screw is threadedly connected inside the mounting plate.

[0033] Furthermore, the preliminary filter also includes:

[0034] Inclined scrapers, wherein two sets of inclined scrapers are provided, and the two sets of inclined scrapers are inclinedly hinged to each other inside the primary filter;

[0035] The elastic torsion spring is provided in four sets, with two sets of elastic torsion springs installed at both ends of a set of inclined scrapers.

[0036] The material collection hopper is provided in two sets, and the two sets of material collection hoppers are fixedly installed at the bottom of the primary filter;

[0037] The collection cylinder is provided in two sets, and the two sets of collection cylinders are connected to the bottom of two sets of collection hoppers by threads.

[0038] Compared with the prior art, the present invention has the following beneficial effects:

[0039] First, this invention features a chondroitin sulfate immobilization system. Through a carrier pretreatment device and an enzyme solution preparation device, the carrier raw materials and animal tissues are processed to form a carrier and an enzyme solution. After the carrier and enzyme solution are fully reacted through an immobilization reaction device, the target product is generated, effectively realizing the efficient immobilization and production of chondroitin sulfate enzyme.

[0040] Secondly, this utility model features a water wheel driven by liquid flowing through a conveying pipe, which causes the cutter to rotate and scrape and cut the surface of the second or first filter plate facing the conveying pipe, preventing residue from clogging and affecting the flow of liquid. It also facilitates the rapid alternation of the second and first filter plates, effectively improving maintenance efficiency. Furthermore, after the alternation, the residue on the second or first filter plate is cleaned and collected, greatly improving cleaning and maintenance efficiency and effectively extending the service life of the primary filter.

[0041] This invention has the advantages of efficient immobilized production, avoidance of clogging, and convenient maintenance. It effectively realizes the efficient immobilized production of chondroitin sulfate enzyme, and greatly improves the cleaning and maintenance efficiency, effectively extending the service life of the primary filter. Attached Figure Description

[0042] Figure 1 This is a schematic diagram of the chondroitin sulfate curing system of this utility model.

[0043] Figure 2 This is a preliminary schematic diagram of the filter structure of this utility model.

[0044] Figure 3 This is a schematic diagram of the rotating shaft structure of this utility model.

[0045] Figure 4 This is a schematic diagram of the mounting plate structure of this utility model.

[0046] Figure 5 This is a schematic diagram of the collecting cylinder structure of this utility model.

[0047] In the diagram, the correspondence between component names and drawing numbers is as follows:

[0048] 10000, Chondroitin Sulfate Curing System; 20000, Carrier Pretreatment Device; 20001, Ultrasonic Cleaner; 20002, Chemical Reactor; 30000, Enzyme Solution Preparation Device; 30001, Tissue Homogenizer; 30002, Preliminary Filter; 1, Conveying Pipeline; 2, Rotating Shaft; 201, Water Wheel; 202, Cutter; 3, Drive Motor; 301, Threaded Screw; 4, Mounting Plate; 401, First Filter Plate; 402, Second Filter Plate; 403, Inclined Scraper; 404, Elastic Torsion Spring; 5, Collection Cylinder; 501, Collection Hopper; 30003, Centrifuge; 30004, Ion Exchange Chromatography Column; 40000, Immobilized Reaction Device; 40001, Static Mixer; 40002, Fixed Bed Reactor; 50000, Constant Flow Pump. Detailed Implementation

[0049] The embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of this utility model.

[0050] Example 1:

[0051] As attached Figure 1 To be continued Figure 5 As shown:

[0052] This invention provides an immobilization production system for chondroitin sulfate enzyme, including a chondroitin sulfate immobilization system 10000.

[0053] The carrier pretreatment device 20000 processes the carrier raw materials.

[0054] Enzyme solution preparation device 30000, which prepares enzyme solution;

[0055] A constant flow pump of 50000 is used to extract the prepared enzyme solution.

[0056] Immobilization reaction apparatus 40000: The immobilization reaction apparatus 40000 reacts enzyme solution and carrier to produce the target product.

[0057] The carrier pretreatment device 20000 includes:

[0058] Ultrasonic Cleaner 20001: Ultrasonic Cleaner 20001 performs ultrasonic cleaning on carrier materials.

[0059] Chemical reactor 20002 is used to mix the cleaned carrier raw material with an activator under constant temperature and pressure conditions to produce an activated carrier.

[0060] The enzyme solution preparation device 30000 includes:

[0061] The 30001 tissue homogenizer uses internal mechanical blades to break down animal tissues, releasing chondroitin sulfate enzymes from the cells into a buffer solution.

[0062] Preliminary filter 30002 is used to perform preliminary filtration on the broken animal tissue to obtain crude enzyme solution.

[0063] Centrifuge 30003 is used to centrifuge the crude enzyme solution, causing most of the solid impurities to precipitate to the bottom of the centrifuge tube, while the supernatant containing chondroitin sulfate is collected for subsequent purification.

[0064] An ion exchange chromatography column 30004 is used to separate and purify the supernatant after centrifugation, resulting in a purified enzyme solution with a certain concentration.

[0065] The immobilization reaction device 40000 includes:

[0066] Static mixer 40001, the static mixer 40001 fully reacts and mixes the enzyme solution and the carrier pretreatment solution;

[0067] The fixed-bed reactor 40002 contains a carrier. The enzyme solution flows through the carrier inside the fixed-bed reactor 40002 at a certain flow rate to fully react and generate the target product.

[0068] The specific usage and function of this embodiment are as follows:

[0069] The ultrasonic cleaner 20001 is used to ultrasonically clean the dirt, impurities and some loosely attached substances on the surface of the carrier material. The chemical reaction vessel 20002 mixes the cleaned carrier material with an activator under constant temperature and pressure conditions to produce an activated carrier, which improves the carrier's ability to bind to chondroitin sulfate enzyme.

[0070] Animal tissue was cut into small pieces and placed in a tissue homogenizer 30001. An appropriate amount of extraction buffer was added, and the homogenizer 30001 was started. The internal mechanical blades disrupted the tissue cells, releasing chondroitin sulfate enzyme into the buffer. A preliminary filter 30002 pre-filtered the disrupted animal tissue to obtain a crude enzyme solution. A centrifuge 30003 centrifuged the crude enzyme solution, causing most solid impurities to precipitate to the bottom of the centrifuge tube. The supernatant containing chondroitin sulfate was collected for subsequent purification. An ion exchange chromatography column 30004 further purified the supernatant, yielding a purified enzyme solution with a specific concentration.

[0071] The enzyme solution and carrier pretreatment solution are put into the static mixer 40001 for thorough reaction and mixing. The mixed liquid is then stably transported to the fixed bed reactor 40002 by the constant flow pump 50000. The fixed bed reactor 40002 is equipped with a carrier. The enzyme solution flows through the carrier inside the fixed bed reactor 40002 at a certain flow rate to fully react and generate the target product.

[0072] Example 2:

[0073] Based on Example 1, such as Figures 1 to 5 As shown, it also includes: a preliminary filter 30002;

[0074] The preliminary filter 30002 includes:

[0075] Delivery pipe 1 is installed between the primary filter 30002 and the tissue homogenizer 30001.

[0076] Rotating shaft 2 is rotatably connected inside conveying pipe 1;

[0077] Water turbine 201 is fixedly installed in the middle of the rotating shaft 2;

[0078] The cutter 202 is fixedly installed at the bottom of the rotating shaft 2. Its function is that when the buffer solution carrying animal tissue flows out of the tissue homogenizer 30001, it passes through the delivery pipe 1 and the preliminary filter 30002 before entering the centrifuge 30003. When the liquid flows through the delivery pipe 1, the water flow drives the water wheel 201 to rotate, the water wheel 201 rotates, the rotating shaft 2 rotates, and the rotating shaft 2 rotates, which in turn drives the cutter 202 to rotate.

[0079] The preliminary filter 30002 also includes:

[0080] Mounting plate 4 is slidably connected inside the primary filter 30002;

[0081] The first filter plate 401 is snapped into the inside of the mounting plate 4;

[0082] The second filter plate 402 is snapped into the inside of the mounting plate 4.

[0083] Drive motor 3 is fixedly installed on one side of the primary filter 30002;

[0084] The threaded screw 301 is rotatably connected inside the primary filter 30002 and fixedly installed at one end of the drive motor 3. The threaded screw 301 is threadedly connected to the inside of the mounting plate 4. Its function is that when liquid flows through the primary filter 30002, the first filter plate 401 in the mounting plate 4, which faces the conveying pipe 1, filters the liquid, intercepting larger animal tissue residues and impurities inside the liquid. After the first filter plate 401 has been used for a long time, the drive motor 3 is energized and drives the threaded screw 301 to rotate. The threaded screw 301 drives the mounting plate 4 to slide inside the primary filter 30002 through the thread, so that the second filter plate 402 and the first filter plate 401 alternate rapidly, so that the second filter plate 402 faces the conveying pipe 1 for filtration.

[0085] The preliminary filter 30002 also includes:

[0086] Inclined scraper 403, two sets of inclined scraper 403 are provided, and the two sets of inclined scraper 403 are inclinedly hinged in opposite directions inside the primary filter 30002;

[0087] Elastic torsion spring 404, four sets of elastic torsion spring 404 are provided, and every two sets of elastic torsion spring 404 are installed at both ends of a set of inclined scraper 403.

[0088] The material collection hopper 501 is provided in two sets, and the two sets of material collection hoppers 501 are fixedly installed at the bottom of the primary filter 30002;

[0089] The collecting cylinder 5 consists of two sets, which are threadedly connected to the bottom of two sets of collecting hoppers 501. Its function is as follows: the elastic torsion spring 404, under its elastic action, drives the inclined scraper 403 to press tightly against the surface of the mounting plate 4. When the second filter plate 402 and the first filter plate 401 alternate rapidly, the first filter plate 401, aligned with the conveying pipe 1, slides out. Due to the inclined structure of the inclined scraper 403 and the elastic operation of the elastic torsion spring 404, the residue inside the first filter plate 401 is not scraped by the inclined scraper 403. The residue adheres to the surface of the first filter plate 401 and enters the inclined side of the inclined scraper 403 along with the first filter plate 401. The mounting plate 4 then drives the second filter plate 402 to slide to the position of the conveying pipe 1. The inclined structure of the inclined scraper 403 scrapes the residue from the surface of the second filter plate 402, preventing the residue from entering the conveying pipe 1. The scraped residue is collected by the collecting hopper 501 and falls into the collecting cylinder 5 for centralized collection.

[0090] The specific usage and function of this embodiment are as follows:

[0091] After the buffer solution carrying animal tissue flows out of the tissue homogenizer 30001, it passes through the preliminary filter 30002 via the delivery pipe 1 and enters the centrifuge 30003. As the liquid flows through the delivery pipe 1, the water flow drives the water wheel 201 to rotate. The rotation of the water wheel 201 drives the rotating shaft 2 to rotate, and the rotation of the rotating shaft 2 drives the cutter 202 to rotate. The rotating cutter 202 scrapes and cuts the surface of the second filter plate 402 or the first filter plate 401 facing the delivery pipe 1 to prevent residue from clogging and affecting the flow of liquid.

[0092] When the liquid flows through the primary filter 30002, the first filter plate 401 in the mounting plate 4, which is directly opposite the conveying pipe 1, filters the liquid, intercepting larger animal tissue residues and impurities inside the liquid. After the first filter plate 401 has been used for a long time, the drive motor 3 is powered on and drives the threaded screw 301 to rotate. The threaded screw 301 drives the mounting plate 4 to slide inside the primary filter 30002 through the thread, so that the second filter plate 402 and the first filter plate 401 alternate rapidly, so that the second filter plate 402 is directly opposite the conveying pipe 1 for filtration.

[0093] Under the elastic action of the elastic torsion spring 404, the inclined scraper 403 is driven to stick tightly to the surface of the mounting plate 4. When the second filter plate 402 and the first filter plate 401 are rapidly alternating, the first filter plate 401, which is aligned with the conveying pipe 1, slides out. Due to the inclined structure of the inclined scraper 403 and the elastic operation of the elastic torsion spring 404, the residue inside the first filter plate 401 is not scraped by the inclined scraper 403. The residue adheres to the surface of the first filter plate 401 and enters the inclined side of the inclined scraper 403 with the first filter plate 401. The mounting plate 4 drives the second filter plate 402 to slide to the position of the conveying pipe 1. The inclined structure of the inclined scraper 403 scrapes the residue on the surface of the second filter plate 402 to prevent the residue from entering the position of the conveying pipe 1. The scraped residue is collected by the collecting hopper 501 and falls into the collecting cylinder 5 for centralized collection.

[0094] The following points should be noted in this article:

[0095] 1. The accompanying drawings of the embodiments disclosed herein only relate to the structures involved in the embodiments disclosed herein; other structures can be referred to in general design.

[0096] 2. Where there is no conflict, the embodiments of this disclosure and the features in the embodiments can be combined with each other to obtain new embodiments.

[0097] The above are merely specific embodiments of this disclosure, but the scope of protection of this disclosure is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this disclosure should be included within the scope of protection of this disclosure. Therefore, the scope of protection of this disclosure should be determined by the scope of the claims.

Claims

1. An immobilized production system for chondroitin sulfate enzyme, characterized in that: The chondroitin sulfate immobilization production system includes a chondroitin sulfate immobilization system (10000); a carrier pretreatment device (20000) for treating carrier raw materials; an enzyme solution preparation device (30000) for preparing enzyme solution; a constant flow pump (50000) for extracting the prepared enzyme solution; and an immobilization reaction device (40000) for reacting the enzyme solution and carrier to produce the target product.

2. The immobilized production system for chondroitin sulfate enzyme as described in claim 1, characterized in that: The carrier pretreatment device (20000) includes an ultrasonic cleaner (20001) and a chemical reactor (20002). The ultrasonic cleaner (20001) performs ultrasonic cleaning on the carrier raw material. The chemical reactor (20002) mixes the cleaned carrier raw material with an activator under constant temperature and pressure conditions to produce an activated carrier.

3. The immobilized production system for chondroitin sulfate enzyme as described in claim 1, characterized in that: The enzyme solution preparation device (30000) includes: a tissue homogenizer (30001), a preliminary filter (30002), a centrifuge (30003), and an ion exchange chromatography column (30004). The tissue homogenizer (30001) breaks down animal tissues using internal mechanical blades, releasing chondroitin sulfate enzymes from the cells into a buffer solution. The preliminary filter (30002) performs preliminary filtration on the broken animal tissues to obtain a crude enzyme solution. The centrifuge (30003) centrifuges the crude enzyme solution, causing most of the solid impurities to precipitate to the bottom of the centrifuge tube, while the supernatant containing chondroitin sulfate is collected for subsequent purification. The ion exchange chromatography column (30004) separates and purifies the supernatant after centrifugation to obtain a purified enzyme solution with a certain concentration.

4. The immobilized production system for chondroitin sulfate enzyme as described in claim 1, characterized in that: The immobilization reaction device (40000) includes a static mixer (40001) and a fixed-bed reactor (40002). The static mixer (40001) fully reacts and mixes the enzyme solution and the carrier pretreatment solution. The fixed-bed reactor (40002) is equipped with a carrier. The enzyme solution flows through the carrier inside the fixed-bed reactor (40002) at a certain flow rate to fully react and generate the target product.

5. The immobilized production system for chondroitin sulfate enzyme as described in claim 3, characterized in that: The preliminary filter (30002) includes: a conveying pipe (1), a rotating shaft (2), a water wheel (201), and a cutter (202). The conveying pipe (1) is installed in the middle of the preliminary filter (30002) and the tissue homogenizer (30001). The rotating shaft (2) is rotatably connected inside the conveying pipe (1). The water wheel (201) is fixedly installed in the middle of the rotating shaft (2). The cutter (202) is fixedly installed at the bottom of the rotating shaft (2).

6. The immobilized production system for chondroitin sulfate enzyme as described in claim 5, characterized in that: The preliminary filter (30002) further includes: a mounting plate (4), a first filter plate (401), a second filter plate (402), a drive motor (3), and a threaded screw (301). The mounting plate (4) is slidably connected inside the preliminary filter (30002); the first filter plate (401) is snapped into the mounting plate (4); the second filter plate (402) is snapped into the mounting plate (4); the drive motor (3) is fixedly installed on one side of the preliminary filter (30002); the threaded screw (301) is rotatably connected inside the preliminary filter (30002), and the threaded screw (301) is fixedly installed at one end of the drive motor (3). The threaded screw (301) is threadedly connected inside the mounting plate (4).

7. The immobilized production system for chondroitin sulfate enzyme as described in claim 5, characterized in that: The preliminary filter (30002) further includes: an inclined scraper (403), an elastic torsion spring (404), a collection hopper (501), and a collection cylinder (5). The inclined scraper (403) is provided in two sets, and the two sets of inclined scraper (403) are inclinedly hinged to each other in opposite directions inside the preliminary filter (30002). The elastic torsion spring (404) is provided in four sets, and every two sets of elastic torsion spring (404) are installed at both ends of a set of inclined scraper (403). The collection hopper (501) is provided in two sets, and the two sets of collection hopper (501) are fixedly installed at the bottom of the preliminary filter (30002). There are two sets of collection cylinders (5), and the two sets of collection cylinders (5) are connected to the bottom of the two sets of collection hoppers (501) by threads.

Images