Freezing device for constructing atrophic meibomian gland dysfunction animal model

Abstract

The utility model provides a freezing device for constructing an atrophic meibomian gland dysfunction animal model, and belongs to the technical field of medicine. The problems that when an existing model is constructed, no matched damage device exists, damage to other parts is difficult to avoid when alkali damage and electrothermal firing are utilized, and operation is difficult are solved. Comprising a detachable nitrogen storage bottle and a bottle cap, the bottle cap is sequentially provided with a copper rod seat and a thermal insulation protection sleeve, a copper rod is arranged in the thermal insulation protection sleeve, one end of the copper rod extends out of the thermal insulation protection sleeve and is provided with a thin probe, the other end of the copper rod penetrates through the bottle cap and then extends into the nitrogen storage bottle, and a wire of a thermometer is in contact connection with the copper rod through a wire opening of the thermal insulation protection sleeve. The meibomian gland atrophy model can be used for constructing an atrophy meibomian gland dysfunction animal model, the blank in the field is filled up, the meibomian gland atrophy cells can be successfully damaged relative to alkali burn and electric heating guide wire injury, the atrophy of the meibomian glands is caused, the secretion function is reduced within a period of time, operation in a slit lamp is not needed, and the operation method is simple.

Description

TECHNICAL FIELD

[0001] The utility model relates to medical technology technical field, and particularly, relate to a kind of for constructing atrophic meibomian gland dysfunction animal model's freezing device. BACKGROUND

[0002] In Chinese meibomian gland dysfunction expert consensus: definition and classification (2023) It is pointed out that an important type in meibomian gland dysfunction is acinar atrophy meibomian gland dysfunction, which is caused by meibomian gland acinar atrophy, and the terminal duct may not be obviously blocked, whether accompanied by duct atrophy needs further study. However, the animal model of this type of atrophic meibomian gland dysfunction is still under development and exploration. In clinical practice, there are also a considerable number of atrophic meibomian gland patients without age-related causes related to abnormal lipid metabolism. For those atrophic meibomian gland dysfunction caused by decreased acinar secretion due to other factors, there is no suitable animal model. Existing atrophic MGD models mainly include gene knockout animal models and aged animal models. Compared with gene knockout animal models and aged animal models, the atrophic meibomian gland dysfunction model constructed by damaging the mouse eyelid has no meibomian gland dysplasia problem in gene knockout mice, has low limitations in studying the pathogenesis, has lower feeding costs than aged animals, has a short feeding cycle; And this modeling method is simpler, faster and repeatable, with a success rate of more than 90% in the experimental process. Correspondingly, the damage device for constructing the atrophic meibomian gland dysfunction animal model has not been proposed.

[0003] In the experimental process, both alkali burn and electric heating wire burning meibomian gland duct opening are considered, which are mechanical damage to the duct opening to produce scar, leading to poor meibum excretion, and further secondary atrophy and loss of meibomian glands. In terms of modeling difficulty and damage to surrounding tissues, the fluidity of strong alkali is relatively strong, and it is difficult to ensure that the liquid will not flow to other parts of the eye to cause damage to other parts. The method of electric heating wire burning meibomian gland duct opening requires very fine electric heating wire, and relatively fine operation is completed on a slit lamp, which has certain requirements for the professional level of the modeling personnel. UTILITY MODEL CONTENTS

[0004] The technical problem to be solved by the utility model is:

[0005] In order to solve the problem that there is no matching damage device during the construction of the existing model, and it is difficult to avoid damage to other parts and operation when using alkali damage and electric heating.

[0006] The technical scheme adopted by the utility model to solve the above technical problems is:

[0007] The utility model provides a kind of for constructing atrophic meibomian gland dysfunction animal model's refrigeration device, including detachably connected nitrogen storage bottle and bottle cap, copper pole seat is equipped on the bottle cap, temperature protection sleeve pipe is equipped on the copper pole seat, copper pole is arranged in temperature protection sleeve pipe, and copper pole one end extends out temperature protection sleeve pipe and is connected with thin copper pole, copper pole other end is inserted into nitrogen storage bottle after penetrating bottle cap, the sidewall of temperature protection sleeve pipe is equipped with wire opening, the wire of thermometer is inserted into wire opening and is contacted with copper pole, it is fixed by limiting clamp in wire opening, the thermometer is electronic ultralow temperature thermometer.

[0008] Further, the copper pole inserted into the nitrogen storage bottle includes a thin copper pole and a spiral copper sleeve sleeved at the bottom end of the thin copper pole.

[0009] Further, the copper pole is provided with a sealing sleeve at the position penetrating the bottle cap.

[0010] Further, it further includes a liquid level meter, which is arranged on the upper cover in the bottle cap or the sealing sleeve.

[0011] Further, the end of the liquid level meter is flush with the end of the copper pole, and the end portions are located 1cm-2cm above the bottom of the nitrogen storage bottle.

[0012] Further, the temperature protection sleeve pipe, the copper pole in the temperature protection sleeve pipe and the thin probe are all inclined to one side.

[0013] Further, the thin probe is a curved arc structure.

[0014] Further, the thin probe is a copper thin probe.

[0015] Further, a display screen is arranged on the outer wall of the bottle cap, and the display screen is connected with the liquid level meter.

[0016] Further, it further includes a pressure relief valve arranged on the bottle cap for pressure relief in the bottle.

[0017] Compared with the prior art, the utility model has the beneficial effects that:

[0018] The utility model relates a kind of freezing device for constructing atrophic meibomian gland dysfunction animal model, including detachable nitrogen storage bottle and bottle cap, copper pole seat and temperature protection sleeve are sequentially equipped on bottle cap, copper pole is equipped in temperature protection sleeve, copper pole one end extends out temperature protection sleeve and is equipped with thin probe, copper pole other end is inserted into nitrogen storage bottle after penetrating bottle cap, the lead of thermometer is contacted with copper pole through the lead opening of temperature protection sleeve;The setting of the present device can be used to construct atrophic meibomian gland dysfunction animal model, make up the blank in the field, and compared with alkali burn and electric heating guide wire damage, can successfully destroy the acinar cell of meibomian gland, cause acinus atrophy and secretory function to decline in a period of time, and experiment proves that, the success rate of the device in model construction is more than 90%, and it does not need in slit lamp operation, and the operation method is simple. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 It is a perspective view of the freezing device for constructing atrophic meibomian gland dysfunction animal model in the utility model embodiment;

[0020] Figure 2 It is a sectional view of the freezing device for constructing atrophic meibomian gland dysfunction animal model in the utility model embodiment Figure 1 ;

[0021] Figure 3 It is a sectional view of the freezing device for constructing atrophic meibomian gland dysfunction animal model in the utility model embodiment Figure 2 ;

[0022] Figure 4 It is the structure diagram of temperature protection sleeve, lead and limit clamp in the utility model embodiment.

[0023] EXPLANATION OF THE DRAWINGS:

[0024] 1, nitrogen storage bottle;2, bottle cap;3, display screen;4, copper pole seat;5, temperature protection sleeve;6, copper pole;7, thin probe;8, lead;9, thermometer;10, liquid level meter;11, limit clamp;12, sealing sleeve;13, spiral copper sleeve;14, liquid nitrogen;15, lead opening;16, limit bolt;17, thin copper pole;18, pressure relief valve. DETAILED DESCRIPTION

[0025] In the description of the utility model, it should be explained that the terms of each embodiment, such as "up", "down", "front", "back", "left", "right" and so on, the words of indicating orientation, only for the position relationship based on the drawing of specification, and not the element and device that is indicated must be operated according to the orientation of specification and the method of limit defined operation and method, structure, the orientation term does not constitute the restriction of the utility model.

[0026] In order to make the above-mentioned purposes, characteristics and advantages of the utility model more obvious and easy to understand, the specific embodiments of the utility model will be described in detail below with reference to the drawings.

[0027] Specific embodiment one: combined Figures 1 to 4 As shown in the utility model provides a kind of for constructing atrophic meibomian gland dysfunction animal model's refrigeration device, including detachable connection's nitrogen storage bottle 1 and bottle cap 2, copper pole seat 4 is equipped on the bottle cap 2, temperature protection sleeve 5 is equipped on the copper pole seat 4, copper pole 6 is arranged in temperature protection sleeve 5, and one end extends out temperature protection sleeve 5 and is connected with thin copper pole 17, the other end is inserted into nitrogen storage bottle 1 after penetrating bottle cap 2, temperature protection sleeve 5's lateral wall is equipped with wire opening 15, the wire 8 of thermometer 9 is inserted into wire opening 15 and is contacted with copper pole 6, it is fixed by limiting clamp 11 at wire opening 15, the opening of limiting clamp 11 is fixed by limiting bolt 16. By copper pole 6 contact liquid nitrogen 14 in nitrogen storage bottle 1, thin copper pole 17 temperature is reduced by the good transmission performance of copper pole 6, reaches-100 ℃±15 ℃ after testing by thermometer 9, and then liquid nitrogen 14 damage can be carried out to the eyelid of mouse. Proved by experiment, liquid nitrogen 14 refrigeration can lead to gland atrophy and secretory function decrease in a period of time, there is meibomian gland dysfunction to cause the increase of inflammatory factor expression, the decrease of Ki67 positive cell content, the decrease of K14 content and the increase of K10 content and Sprr1b in hyperkeratosis characterization. As can be known, the atrophic meibomian gland dysfunction animal model constructed using the device meets the research standard, and the thermometer 9 is an electronic ultra-low temperature thermometer.

[0028] Preferably, the copper pole 6 inserted into the nitrogen storage bottle 1 includes a thin copper pole 17 and a spiral copper sleeve 13 sleeved at the bottom end of the thin copper pole 17. The spiral copper sleeve 13 can increase the contact area with the liquid nitrogen 14 in the nitrogen storage bottle 1, so that the thin probe 7 can be quickly cooled.

[0029] Preferably, the copper pole 6 penetrating the bottle cap 2 is provided with a sealing sleeve 12. The sealing sleeve 12 can be filled with glue to prevent the liquid nitrogen 14 from escaping from the gap of the connection and to avoid the liquid nitrogen 14 from being consumed too quickly.

[0030] Preferably, the liquid level meter 10 is arranged on the upper cover of the bottle cap 2 or the sealing sleeve 12. The end of the liquid level meter 10 is flush with the end of the copper pole 6, and the ends are located 1-2 cm above the bottom of the nitrogen storage bottle 1. The liquid level meter 10 can fully measure the liquid level of the liquid nitrogen 14, and the copper pole 6 can easily contact the liquid nitrogen 14 to maintain a low temperature for a longer time.

[0031] Preferably, the temperature protection sleeve 5, the copper rod 6 located in the temperature protection sleeve 5 and the fine probe 7 are all inclined to one side, and the fine probe 7 is in a curved arc structure, so as to facilitate the fine probe 7 to cause the liquid nitrogen 14 to damage the eyelid of the mouse.

[0032] Preferably, the fine probe 7 is a copper fine probe, which also has good heat transfer performance.

[0033] Preferably, the outer wall of the bottle cap 2 is provided with a display screen 3, the display screen 3 is connected with the liquid level meter 10 and the timer, the display screen 3 is used to display the liquid level of the liquid nitrogen 14, and can also be timed, so as to facilitate the control of the time of the liquid nitrogen 14 damage.

[0034] Preferably, the bottle cap 2 is provided with a pressure relief valve 18, the pressure relief valve 18 is used to discharge the nitrogen gas after the liquid nitrogen in the bottle is vaporized, so as to avoid that the pressure in the bottle is too large, and a pressure gauge can also be provided, the pressure gauge is used to measure the pressure in the bottle, when the pressure in the bottle is too large, the exhaust can be used by the pressure relief valve 18, in order to avoid that the discharged nitrogen gas damages the lead wire 8, the lead wire 8 needs to be away from the exhaust port when the exhaust is performed.

[0035] Operation method:

[0036] 8-week-old male C57 / BL6 mice were obtained from Changchun Biotechnology (China). The experimental procedures were performed in accordance with the Statement on the Use of Animals in Ophthalmic and Visual Research of the Association for Research in Vision and Ophthalmology (ARVO) and approved by the Animal Ethics Committee of Harbin Medical University. Animals had free access to standard rodent chow and water and were maintained in a standard pathogen-free environment at 25°C ± 1°C, 60% ± 10% relative humidity, with alternating 12-hour light-dark cycles (from 8:00 am to 8:00 pm).

[0037] Under general anesthesia, liquid nitrogen was placed in the nitrogen storage bottle 1 and the bottle cap was screwed tightly, the temperature was observed to be reduced to -100°C by the thermometer 9, and the specially designed freezing device was applied to the edges of the upper eyelid and the lower eyelid, the fine probe 7 was in contact with the eyelid margin for 30 seconds, and the contact part was about two-thirds of the central part of the eyelid margin, at this time, the temperature was observed to be reduced to below -115°C, and then the operation was performed again. When the temperature is too low after the liquid nitrogen damages multiple mice, the bottle cap 2 carrying the copper rod 6 can be removed, and the operation can be performed again when the temperature rises to -100°C.

[0038] Although the utility model discloses as above, the protection scope of the utility model discloses not only limit to this. The utility model field technical personnel can make various changes and modifications under the premise of not departing from the spirit and scope of the utility model discloses, and these changes and modifications will fall into the protection scope of the utility model.

Claims

1. A freezing device for constructing an atrophic type meibomian gland dysfunction animal model, characterized by: The application relates to a detachable nitrogen storage bottle (1) and a bottle cap (2), wherein a copper rod base (4) is arranged on the bottle cap (2), a temperature insulation protective sleeve (5) is arranged on the copper rod base (4), a copper rod (6) is arranged in the temperature insulation protective sleeve (5), one end of the copper rod (6) extends out of the temperature insulation protective sleeve (5) and is connected with a thin copper rod (17), the other end of the copper rod (6) extends into the nitrogen storage bottle (1) through the bottle cap (2), a lead wire opening (15) is arranged on the side wall of the temperature insulation protective sleeve (5), a lead wire (8) of a thermometer (9) extends into the lead wire opening (15) and is in contact with the copper rod (6), the lead wire opening (15) is fixed through a limiting clamp (11), and the thermometer (9) is an electronic ultra-low temperature thermometer.

2. The freezing device for constructing an atrophic type meibomian gland dysfunction animal model according to claim 1, characterized in that: The copper rod (6) extending into the nitrogen storage bottle (1) comprises the thin copper rod (17) and a spiral copper sleeve (13) arranged at the bottom end of the thin copper rod (17).

3. The freezing device for constructing an atrophic type meibomian gland dysfunction animal model according to claim 2, characterized in that: A sealing sleeve (12) is arranged at the position where the copper rod (6) penetrates through the bottle cap (2).

4. The freezing device for constructing an atrophic type meibomian gland dysfunction animal model according to claim 3, characterized in that: A liquid level meter (10) is further arranged on the upper cover in the bottle cap (2) or the sealing sleeve (12).

5. The freezing device for constructing an atrophic type meibomian gland dysfunction animal model according to claim 4, characterized in that: The end of the liquid level meter (10) is flush with the end of the copper rod (6), and the end portions are located at 1cm-2cm above the bottom of the nitrogen storage bottle (1).

6. The freezing device for constructing an atrophic type meibomian gland dysfunction animal model according to claim 5, characterized in that: The temperature insulation protective sleeve (5), the copper rod (6) and a thin probe (7) in the temperature insulation protective sleeve (5) are all inclined to one side.

7. The freezing device for constructing an atrophic type meibomian gland dysfunction animal model according to claim 6, characterized in that: The thin probe (7) is a curved arc structure.

8. The freezing device for constructing an atrophic type meibomian gland dysfunction animal model according to claim 7, characterized in that: The thin probe (7) is a copper thin probe.

9. The freezing device for constructing an atrophic type meibomian gland dysfunction animal model according to claim 8, characterized in that: A display screen (3) is arranged on the outer wall of the bottle cap (2), and the display screen (3) is connected with the liquid level meter (10).

10. The freezing device for constructing an atrophic type meibomian gland dysfunction animal model according to claim 9, characterized in that: A pressure relief valve (18) is further arranged on the bottle cap (2) and used for relieving the pressure in the bottle.

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