Microfluidic chip

Abstract

The utility model relates to the technical field of microfluidic structures, and provides a microfluidic chip, which comprises a filter membrane layer, a first plate body and a second plate body, the first plate body is provided with a first end face and a first side face, the first end face is recessed to form a first groove structure, the first side face is provided with a first sample inlet and a first sample outlet, and the second plate body is provided with a second groove structure. The first sample inlet and the first sample outlet are both communicated with the first groove structure; the second plate body is provided with a second end face and a second side face, the second end face is sunken to form a second groove structure, a second sample inlet and a second sample outlet are formed in the second side face, and the second sample inlet and the second sample outlet are both communicated with the second groove structure. According to the micro-fluidic chip provided by the utility model, the characteristics of the filtering membrane layer are fully utilized, and the stimulation and interference on the fluid shear force of a sample in the second plate body when fluid is injected into the first plate body can be greatly reduced on the premise of ensuring the material exchange between the first plate body and the second plate body.

Description

TECHNICAL FIELD

[0001] The utility model relates to micro -fluidic structure technical field especially provides a kind of micro -fluidic chip. BACKGROUND

[0002] Microfluidic is a kind of technology to control fluid in micron scale space. As an important branch of microfluidic chip research, droplet microfluidic is developed in recent years on the basis of traditional microfluidic system. Droplet microfluidic technology can prepare monodisperse picoliter droplets. Different samples, including chemical reagents, micro-nanoparticles, cells, DNA and microorganisms, can be wrapped in droplets. This makes droplets be able to be used as independent reaction units for qualitative or quantitative detection, with advantages of small sample requirement, portability and high integration. Currently, it is widely used in biomedical field.

[0003] However, the microfluidic chip in the related product is difficult to control the stimulation and interference of fluid shear force, thereby causing deviation of data in the related experiment and affecting the experimental results. UTILITY MODEL CONTENT

[0004] The utility model discloses a kind of microfluidic chips, to improve the problem that the fluid shear force size of existing microfluidic chip is difficult to control.

[0005] To achieve the above object, the technical scheme adopted by the utility model is as follows:

[0006] The application embodiment provides a kind of microfluidic chip, comprising:

[0007] Filter membrane layer;

[0008] First plate body, the first plate body has first end face and the first side surface around the first end face, the first end face is recessed to form first groove structure, the first side surface is equipped with first sample inlet and first sample outlet, the first sample inlet and first sample outlet are all communicated with the first groove structure;And

[0009] Second plate body, the second plate body has second end face and the second side surface around the second end face, which are arranged opposite to the first end face, the second end face is recessed to form second groove structure, the second side surface is equipped with second sample inlet and second sample outlet, the second sample inlet and the second sample outlet are all communicated with the second groove structure;

[0010] Wherein, in the first direction, the first plate body is covered on the second plate body, the first groove structure and the second groove structure are oppositely arranged, the filter membrane layer is located between the first plate body and the second plate body, and in the first direction, the projection of the first groove structure and the projection of the second groove structure all fall into the projection range of the filter membrane layer.

[0011] The microfluidic chip has the beneficial effects that: the filter membrane layer is additionally arranged between the first end face of the first plate body and the second end face of the second plate body, the characteristics of the filter membrane layer are fully utilized, that is, the fluid shear force stimulation and interference of the sample in the second plate body when the first plate body injects fluid can be greatly reduced under the premise of guaranteeing the material exchange between the first plate body and the second plate body.

[0012] In some embodiments, the radius of the first sample inlet is 0.3mm-0.5mm; and / or, the radius of the first sample outlet is 0.3mm-0.5mm.

[0013] In some embodiments, the radius of the second sample inlet is 0.3mm-0.5mm; and / or, the radius of the second sample outlet is 0.3mm-0.5mm.

[0014] In some embodiments, the first sample inlet and the first sample outlet are respectively located on two opposite first side faces; and / or,

[0015] The second sample inlet and the second sample outlet are respectively located on two opposite second side faces.

[0016] In some embodiments, in the first direction, the projection profile of the first groove structure is an ellipse, the projection profile of the second groove structure is an ellipse, the projection profile of the first groove structure and the projection profile of the second groove structure are concentrically arranged, and the long axis extension direction of the first groove structure is perpendicular to the long axis extension direction of the second groove structure.

[0017] In some embodiments, the connecting line of the first sample inlet and the first sample outlet coincides with the long axis of the first groove structure; and / or,

[0018] The connecting line of the second sample inlet and the second sample outlet coincides with the long axis of the second groove structure.

[0019] In some embodiments, the second groove structure is provided with a micropore structure on the groove ground, and the micropore structure comprises a plurality of blind holes arranged in an array.

[0020] In some embodiments, in the first direction, the projection of the micropore structure is located in the range where the projection of the first groove structure and the projection of the second groove structure coincide.

[0021] In some embodiments, the blind hole comprises a circular hole part and a semi-spherical hole part connected with the circular hole part, wherein the depth of the circular hole part is 0.3mm-0.5mm, the radius of the circular hole part is 0.3mm-0.5mm; the radius of the semi-spherical hole part is 0.3mm-0.5mm.

[0022] In some embodiments, the pore size of the filter membrane layer ranges from 0.1μm to 50μm. BRIEF DESCRIPTION OF DRAWINGS

[0023] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed in the embodiments or prior art description will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative effort on the basis of these drawings.

[0024] Figure 1 An exploded view of the microfluidic chip provided by the embodiments of the present application is shown in the figure.

[0025] Figure 2 A structural schematic view of the first plate body of the microfluidic chip provided by the embodiments of the present application is shown in the figure.

[0026] Figure 3 A structural schematic view of the second plate body of the microfluidic chip provided by the embodiments of the present application is shown in the figure.

[0027] Figure 4 A cross-sectional view of the microfluidic chip provided by the embodiments of the present application is shown in the figure.

[0028] In the figure, various reference signs are as follows:

[0029] 10, filter membrane layer;

[0030] 20, first plate body; 20a, first end face; 20b, first side face; 20c, first groove structure; 20d, first sample inlet; 20e, first sample outlet;

[0031] 30, second plate body; 30a, second end face; 30b, second side face; 30c, second groove structure; 30d, second sample inlet; 30e, second sample outlet;

[0032] 40, microporous structure; 41, blind hole; 411, circular hole part; 412, semi-spherical hole part. DETAILED DESCRIPTION

[0033] The embodiments of the present application are described below in detail, examples of the embodiments are shown in the drawings, wherein the same or similar notations represent the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary, and are intended to explain the present application, and cannot be understood as a limitation of the present application.

[0034] In the description of the present application, it should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation of the present application.

[0035] In addition, the terms "first", "second" are only for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features defined with "first", "second" can explicitly or implicitly include one or more of the features. In the description of the present application, the meaning of "multiple" is two or more, unless otherwise specifically limited.

[0036] In the present application, unless otherwise specifically defined and limited, the terms "mounting", "connection", "connection", "fixing" and the like should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or it can be integrated; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the internal communication of two elements or the interaction relationship between two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0037] Please refer to Figures 1 to 4 The embodiment of the present application provides a microfluidic chip, which comprises a filter membrane layer 10, a first plate body 20 and a second plate body 30.

[0038] Among them, the filter membrane layer 10 has good filtering characteristics, for example, in some cell culture experiments, the filter membrane layer 10 can adopt polycarbonate (PC), polyethylene terephthalate (PET) and other materials, and the hydrophilicity of the filter membrane layer 10 can be adjusted to improve the degree of cell adhesion on its surface in actual different use requirements.

[0039] The first plate body 20 and the second plate body 30 are main body structures of the microfluidic chip, and can be made of polystyrene (PS) or polymethyl methacrylate (PMMA) and the like to ensure good biocompatibility; in use, the first plate body 20 and the second plate body 30 are arranged in an up-down orientation.

[0040] Specifically, the first plate body 20 has a first end face 20a and a first side face 20b surrounding the first end face 20a, the first end face 20a is recessed to form a first groove structure 20c, the first side face 20b is provided with a first sample inlet 20d and a first sample outlet 20e, and the first sample inlet 20d and the first sample outlet 20e are both in communication with the first groove structure 20c; and

[0041] The second plate body 30 has a second end face 30a opposite to the first end face 20a and a second side face 30b surrounding the second end face 30a, the second end face 30a is recessed to form a second groove structure 30c, the second side face 30b is provided with a second sample inlet 30d and a second sample outlet 30e, and the second sample inlet 30d and the second sample outlet 30e are both in communication with the second groove structure 30c.

[0042] In the first direction, that is, the thickness direction of the first plate body or the second plate body, the first plate body 20 is arranged on the second plate body 30, the first groove structure 20c and the second groove structure 30c are arranged oppositely, and the filter membrane layer 10 is located between the first plate body 20 and the second plate body 30, and in the first direction, the projection of the first groove structure 20c and the projection of the second groove structure 30c both fall within the projection range of the filter membrane layer 10.

[0043] It can be understood that the first end surface 20a is an end surface of the first plate body 20 facing the second plate body 30, the second end surface 30a is an end surface of the second plate body 30 facing the first plate body 20, and the filter membrane layer 10 is located between the two end surfaces when the two are overlapped; the first side surface 20b is a peripheral side surface of the first plate body 20, and it should be understood that the first plate body 20 has a certain thickness, and similarly, the second side surface 30b is a peripheral side surface of the second plate body 30, so that the number of the first side surface 20b can be one or more according to the shape profile of the first plate body 20 and the second plate body 30, and similarly, the number of the second side surface 30b can be one or more; the first groove structure 20c is formed by recessing the first end surface 20a, and the second groove structure 30c is formed by recessing the second end surface 30a, and the groove openings of the first groove structure 20c and the second groove structure 30c are opposite when the first plate body 20 and the second plate body 30 are overlapped, and optionally, the groove shape profile and size of the first groove structure 20c are completely the same as those of the second groove structure 30c, so that the first groove structure 20c and the second groove structure 30c form a relatively sealed cavity when the first plate body 20 and the second plate body 30 are overlapped, or the groove shape profile and size of the first groove structure 20c are different from those of the second groove structure 30c, and the first groove structure 20c and the second groove structure 30c are misaligned when the first plate body 20 and the second plate body 30 are overlapped.

[0044] In actual use, sample fluid can enter the first groove structure 20c from the first sample inlet 20d, and the filtrate of the sample fluid enters the second groove structure 30c under the filtering effect of the filter membrane layer 10, and meanwhile, the sample fluid can also flow out from the first sample outlet 20e; and similarly, the fluid to be supplemented can enter the second groove structure 30c from the second sample inlet 30d, and then flow out from the second sample outlet 30e.

[0045] The microfluidic chip provided by the utility model, between the first end surface of the first plate body 20 and the second end surface 30a of the second plate body 30, the filter membrane layer 10 is additionally arranged, the characteristics of the filter membrane layer 10 are fully utilized, that is, under the premise of guaranteeing the material exchange between the first plate body 20 and the second plate body 30, the stimulation and interference of the fluid shear force of the sample in the second plate body 30 when the first plate body 20 injects fluid can be greatly reduced.

[0046] In some embodiments, the filter membrane layer 10 can be a polycarbonate material, and the filter membrane layer 10 can be pretreated to have better cell adhesion effect, so as to be suitable for the stable culture of some cells with poor adhesion ability in a fluid environment. For example, the filter membrane layer is immersed in a fibronectin solution overnight. In addition, the material of the first plate body and the second plate body is a polystyrene material.

[0047] In some embodiments, the radius of the first inlet port 20d is 0.3mm-0.5mm; and / or, the radius of the first outlet port 20e is 0.3mm-0.5mm.

[0048] It can be appreciated that the radius of the first inlet port 20d can be 0.3mm, 0.31mm, 0.32mm, 0.33mm, 0.34mm, 0.35mm, 0.36mm, 0.37mm, 0.38mm, 0.39mm, 0.40mm, 0.41mm, 0.42mm, 0.43mm, 0.44mm, 0.45mm, 0.46mm, 0.47mm, 0.48mm, 0.49mm, 0.5mm, etc.

[0049] Similarly, the radius of the first outlet port 20e can be 0.3mm, 0.31mm, 0.32mm, 0.33mm, 0.34mm, 0.35mm, 0.36mm, 0.37mm, 0.38mm, 0.39mm, 0.40mm, 0.41mm, 0.42mm, 0.43mm, 0.44mm, 0.45mm, 0.46mm, 0.47mm, 0.48mm, 0.49mm, 0.5mm, etc.

[0050] In some embodiments, the radius of the second inlet port 30d is 0.3mm-0.5mm; and / or, the radius of the second inlet port 30d is 0.3mm-0.5mm.

[0051] It can be appreciated that the radius of the second inlet port 30d can be 0.3mm, 0.31mm, 0.32mm, 0.33mm, 0.34mm, 0.35mm, 0.36mm, 0.37mm, 0.38mm, 0.39mm, 0.40mm, 0.41mm, 0.42mm, 0.43mm, 0.44mm, 0.45mm, 0.46mm, 0.47mm, 0.48mm, 0.49mm, 0.5mm, etc.

[0052] Similarly, the radius of the second outlet port 30e can be 0.3mm, 0.31mm, 0.32mm, 0.33mm, 0.34mm, 0.35mm, 0.36mm, 0.37mm, 0.38mm, 0.39mm, 0.40mm, 0.41mm, 0.42mm, 0.43mm, 0.44mm, 0.45mm, 0.46mm, 0.47mm, 0.48mm, 0.49mm, 0.5mm, etc.

[0053] It can be appreciated that the radius of the second outlet port 30e can be 0.3mm, 0.31mm, 0.32mm, 0.33mm, 0.34mm, 0.35mm, 0.36mm, 0.37mm, 0.38mm, 0.39mm, 0.40mm, 0.41mm, 0.42mm, 0.43mm, 0.44mm, 0.45mm, 0.46mm, 0.47mm, 0.48mm, 0.49mm, 0.5mm, etc. Figures 1 to 4 In some embodiments, the first inlet port 20d and the first outlet port 20e are respectively located on the two opposite first side faces 20b; and / or,

[0054] The second inlet 30d and the second outlet 30e are respectively arranged on the two opposite second sides 30b.

[0055] It can be understood that the two first sides 20b are arranged oppositely, and the first plate body 20 is optionally a cube, so that the first plate body 20 has four first sides 20b, wherein each of the first sides 20b corresponds to another, and thus two opposite first sides 20b are selected to arrange the first inlet 20d and the first outlet 20e thereon respectively; similarly, when the second plate body 30 is a cube, it also has four second sides 30b, each of the second sides 30b corresponds to another, and thus two opposite second sides 30b are selected to arrange the second inlet 30d and the second outlet 30e thereon respectively.

[0056] The opposite arrangement of the inlet and the outlet can improve the flowability of the sample fluid in the first groove structure 20c or the second groove structure 30c, so as to meet the requirement of rapid inflow and outflow of the sample fluid.

[0057] Please refer to Figures 1 to 4 In some embodiments, in the first direction, the projection profile of the first groove structure 20c is an ellipse, the projection profile of the second groove structure 30c is an ellipse, the projection profile of the first groove structure 20c is arranged concentrically with the projection profile of the second groove structure 30c, and the long axis extension direction of the first groove structure 20c is perpendicular to the long axis extension direction of the second groove structure 30c.

[0058] It can be understood that, in the present embodiment, the first direction is the height direction when the first plate body 20 and the second plate body 30 are buckled, the projections of the two groove structures in the first direction are both ellipses, and the long axis extension direction of the first groove structure 20c is perpendicular to the long axis extension direction of the second groove structure 30c, so that in the first direction, the projections of the two groove structures are arranged staggered and partially overlapped.

[0059] Please refer to Figures 1 to 4 In some embodiments, the line connecting the first inlet 20d and the first outlet 20e coincides with the long axis of the first groove structure 20c; and / or,

[0060] The line connecting the second inlet 30d and the second outlet 30e coincides with the long axis of the second groove structure 30c.

[0061] It can be understood that the extension direction of the first inlet 20d is 180° to the extension direction of the first outlet 20e; similarly, the extension direction of the first inlet 20d is 180° to the extension direction of the first outlet 20e.

[0062] Please refer to Figures 1 to 4In some embodiments, the second groove structure 30c is provided with a micropore structure 40 on the groove ground, and the micropore structure 40 comprises a plurality of blind holes 41 arranged in an array.

[0063] It can be understood that the micropore structure 40 is used for cell culture, and in particular, the micropore structure 40 is composed of a plurality of blind holes 41 arranged in an array.

[0064] Please refer to Figures 1 to 4 In some embodiments, in the first direction, the projection of the micropore structure 40 is located in the range where the projection of the first groove structure 20c and the projection of the second groove structure 30c coincide.

[0065] Please refer to Figures 1 to 4 In some embodiments, the blind hole 41 comprises a circular hole part 411 and a semispherical hole part 412 connected with the circular hole part 411, wherein the depth of the circular hole part 411 is 0.3mm-0.5mm, the radius of the circular hole part 411 is 0.3mm-0.5mm; the radius of the semispherical hole part 412 is 0.3mm-0.5mm.

[0066] In some embodiments, the pore size of the filter membrane layer 10 ranges from 0.1μm to 50μm.

[0067] In a specific embodiment, please refer to Figures 1 to 4 The microfluidic chip comprises a filter membrane layer 10, a first plate body 20 and a second plate body 30.

[0068] The first plate body 20 has a first end face 20a and four first side faces 20b surrounding the first end face 20a; the opposite two first side faces 20b are respectively provided with a first sample inlet 20d and a first sample outlet 20e, and the first groove structure 20c is recessed on the first end face 20a; the second plate body 30 has a second end face 30a and four second side faces 30b surrounding the second end face 30a; the opposite two second side faces 30b are respectively provided with a second sample inlet 30d and a second sample outlet 30e, and the second groove structure 30c is recessed on the second end face 30a.

[0069] In the first direction, the projection profile of the first groove structure 20c is elliptical; the projection profile of the second groove structure 30c is also elliptical, and the projection profile of the first groove structure 20c and the projection profile of the second groove structure 30c are concentrically arranged, and the long axis extension direction of the first groove structure 20c is perpendicular to the long axis extension direction of the second groove structure 30c.

[0070] The line connecting the first sample inlet 20d and the first sample outlet 20e coincides with the long axis of the first groove structure 20c, and the line connecting the second sample inlet 30d and the second sample outlet 30e coincides with the long axis of the second groove structure 30c. The radius of the first sample inlet 20d is 0.4 mm. The radius of the first sample outlet 20e is 0.4 mm. The radius of the second sample inlet 30d is 0.4 mm. The radius of the second sample inlet 30d is 0.4 mm.

[0071] The second groove structure 30c is provided with a micropore structure 40 on the groove ground, and the micropore structure 40 comprises a plurality of blind holes 41 arranged in an array. The blind hole 41 comprises a circular hole part 411 and a semispherical hole part 412 in communication with the circular hole part 411, wherein the depth of the circular hole part 411 is 0.4 mm, the radius of the circular hole part 411 is 0.4 mm, and the radius of the semispherical hole part 412 is 0.4 mm.

[0072] The pore size range of the filter membrane layer 10 is 1 μm. In addition, the number of the filter membrane layer 10 is one.

[0073] The above is only a preferred embodiment of the present application, and is not intended to limit the present application. Any modification, equivalent replacement and improvement within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A microfluidic chip, characterized by, The filter membrane layer is arranged between the first plate body and the second plate body in the first direction. The first plate body has a first end surface and a first side surface surrounding the first end surface, the first end surface is recessed to form a first groove structure, the first side surface is provided with a first sample inlet and a first sample outlet, and the first sample inlet and the first sample outlet are in communication with the first groove structure. The second plate body has a second end surface opposite to the first end surface and a second side surface surrounding the second end surface, the second end surface is recessed to form a second groove structure, the second side surface is provided with a second sample inlet and a second sample outlet, and the second sample inlet and the second sample outlet are in communication with the second groove structure. In the first direction, the first plate body is arranged on the second plate body, the first groove structure and the second groove structure are arranged oppositely, and the filter membrane layer is arranged between the first plate body and the second plate body. The radius of the first sample inlet is 0.3mm-0.5mm; and / or the radius of the first sample outlet is 0.3mm-0.5mm.

2. The microfluidic chip of claim 1, wherein: The radius of the second sample inlet is 0.3mm-0.5mm; and / or the radius of the second sample outlet is 0.3mm-0.5mm.

3. The microfluidic chip of claim 1, wherein: The first sample inlet and the first sample outlet are respectively arranged on two opposite first side surfaces; and / or 4. The microfluidic chip of claim 1, wherein: The second sample inlet and the second sample outlet are respectively arranged on two opposite second side surfaces. In the first direction, the projection contour of the first groove structure is in an elliptical shape, the projection contour of the second groove structure is in an elliptical shape, the projection contour of the first groove structure is concentrically arranged with the projection contour of the second groove structure, and the long axis extension direction of the first groove structure is perpendicular to the long axis extension direction of the second groove structure.

5. The microfluidic chip of claim 4, wherein: The line connecting the first sample inlet and the first sample outlet is coincident with the long axis of the first groove structure; and / or 6. The microfluidic chip of claim 5, wherein: The line connecting the second sample inlet and the second sample outlet is coincident with the long axis of the second groove structure. The second groove structure is provided with a micropore structure on the groove ground, and the micropore structure includes a plurality of blind holes arranged in an array.

7. The microfluidic chip according to any one of claims 1 to 6, wherein: In the first direction, the projection of the micropore structure is located in the range where the projection of the first groove structure and the projection of the second groove structure coincide.

8. The microfluidic chip of claim 7, wherein: The blind hole includes a circular hole part and a semispherical hole part in communication with the circular hole part, wherein the depth of the circular hole part is 0.3mm-0.5mm, the radius of the circular hole part is 0.3mm-0.5mm, and the radius of the semispherical hole part is 0.3mm-0.5mm.

9. The microfluidic chip of claim 7, wherein: The pore size of the filter membrane layer ranges from 0.1μm to 50μm.

10. The microfluidic chip according to any one of claims 1 to 6, wherein: ​

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