Automatic extraction device for primary cells

Abstract

The utility model relates to the technical field of biological medicine, in particular to an automatic primary cell extraction device which comprises a rack, a worktable arranged on the rack, a transfer module, a tissue crushing module, a centrifugation module and a quality inspection module, a to-be-treated tissue sample is fed into the tissue crushing module through the transfer module for cutting, grinding and digesting the sample tissue, then the sample tissue is centrifuged through the centrifugation module so as to obtain a single-cell suspension, and whether the tissue treatment is sufficient or not is judged through the quality inspection module. According to the method, the corresponding organoid single-cell suspension is obtained, manual operation intervention is not needed, experimental errors are reduced, and the working efficiency is greatly improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological medicine technical field especially, relate to a kind of primary cell automation extraction device. BACKGROUND

[0002] Organoid technology is a new type of tissue organ in vitro culture technology produced by introducing the self-assembly characteristics of stem cells into three-dimensional cell culture under the background of continuous deepening of mammalian development, tissue homeostasis and extracellular matrix research, and increasing experience of stem cell culture. Currently, the culture of organoids is still at the laboratory stage. A series of pretreatments are needed before organoid culture. Since errors are inevitable in current manual pretreatment, current cell extraction before culture relies more on manual operation rather than automation. Not only does it require a large amount of learning and training costs, but also manual experimental operation is prone to errors, which cannot achieve the expected results. How to stably and reliably extract cells before organoid culture has become a problem to be solved in the field. SUMMARY

[0003] The utility model aims at the deficiency of prior art and provides a kind of primary cell automation extraction device, can complete the cell automation extraction process before organoid culture, obtain corresponding organoid single cell suspension, and do not need manual operation intervention, reduce experimental error.

[0004] To achieve the above object, the utility model adopts the technical scheme of a kind of primary cell automation extraction device, including rack, workbench being set on rack, transfer module, organization crushing module, centrifugal module, quality inspection module;The workbench is used to store consumables and provide working area for experimental operation, the organization crushing module is used to cut, grind, digest sample tissue to obtain single cell suspension, the centrifugal module is used to carry out high-purity separation, concentration, refining to sample solution to obtain the single cell suspension meeting the demand, the quality inspection module is used to detect whether organization processing is completed, and the transfer module is used to transfer sample and processed single cell suspension between organization crushing module, centrifugal module and quality inspection module.

[0005] Further improvement of the above scheme is that the organization crushing module includes an organization processing instrument arranged on the rack, and the centrifugal module includes a centrifuge arranged on the rack.

[0006] Further improvement of the above scheme is that the organization processing instrument includes an organization processing tube, an organization processing channel and a heating jacket, the organization processing tube is used to hold the organization to be processed and the organization lysis solution and / or enzyme used to process the organization, the organization processing channel is used to install the organization processing tube, and the heating jacket is used to heat the organization processing tube.

[0007] Further, the tissue processing channel is provided with a plurality of.

[0008] Further, the transfer module comprises a pipette transfer device, a plate transfer device and a pipetting device, the pipette transfer device is used for clamping and opening the cover, the plate transfer device is used for transporting the microplate, and the pipetting device is used for pipetting.

[0009] Further, the pipette transfer device comprises an XYZ-axis movement driving assembly arranged on a rack, a pipette assembly support seat, and a pipette assembly rotatably arranged on the pipette assembly support seat, the XYZ-axis movement driving assembly is drivingly connected to the pipette assembly support seat; the pipette assembly comprises a rotation driving assembly rotatably arranged on the support seat, a rotation seat rotatably connected to the power output end of the rotation driving assembly, a pipette driving assembly arranged on the rotation seat, and a pipette claw slidingly connected to the rotation seat in the horizontal direction, the pipette driving assembly is drivingly connected to the pipette claw, and an anti-skid pattern is further arranged on the pipette claw.

[0010] Further, the plate transfer device comprises a multi-axis plate manipulator assembly, the multi-axis plate manipulator assembly comprises an X-axis sliding rail arranged on a rack, an X-axis driving assembly, a Y-axis sliding support seat slidingly arranged on the X-axis sliding rail in the X-axis direction, a Y-axis driving assembly, and a plate hand support assembly slidingly arranged on the Y-axis sliding support seat in the Y-axis direction, the X-axis driving assembly is drivingly connected to the Y-axis sliding support seat, and the Y-axis driving assembly is drivingly connected to the plate hand support assembly; the plate hand support assembly comprises a plate hand support seat, a plate hand sliding seat slidingly arranged on the plate hand support seat in the Y-axis direction, a plate hand sliding driving assembly arranged on the plate hand support seat, a plate hand rotation seat rotatably arranged on the plate hand sliding seat in the horizontal direction, a plate hand rotation driving assembly arranged on the plate hand sliding seat, two L-shaped clamping claws oppositely slidingly arranged on the plate hand sliding seat in the X-axis direction, and an L-shaped clamping claw driving assembly arranged on the plate hand rotation seat, the plate hand sliding driving assembly is drivingly connected to the plate hand sliding seat, the plate hand rotation driving assembly is drivingly connected to the plate hand rotation seat, and the L-shaped clamping claw driving assembly is drivingly connected to the L-shaped clamping claw.

[0011] Further improvement of the above scheme is that the clamp plate transferring device further comprises a rotating plate transferring assembly arranged on the top of the rack, the rotating plate transferring assembly comprises a rotating plate transferring sliding rail arranged on the rack, a rotating plate transferring sliding seat slidably connected to the rotating plate transferring sliding rail, a rotating plate transferring sliding driving assembly arranged on the rotating plate transferring sliding rail, a Z-axis sliding arm slidably connected to the rotating plate transferring sliding rail along the Z-axis direction, a Z-axis sliding arm driving assembly arranged on the rotating plate transferring sliding seat, a rotating plate supporting hand assembly rotatably connected to the bottom of the Z-axis sliding arm, and a rotating plate supporting hand rotating driving assembly arranged on the Z-axis sliding arm, the rotating plate transferring sliding driving assembly is drivingly connected to the rotating plate transferring sliding seat, the Z-axis sliding arm driving assembly is drivingly connected to the Z-axis sliding arm, and the rotating plate supporting hand rotating driving assembly drives the rotating plate supporting hand assembly to rotate.

[0012] Further improvement of the above scheme is that the pipetting device comprises a pipetting manipulator sliding rail arranged on the rack along the X-axis direction, a pipetting manipulator sliding seat slidably arranged on the pipetting manipulator sliding rail along the X-axis direction, a pipetting manipulator support arranged on the pipetting manipulator sliding seat, a pipette slidably arranged on the pipetting support along the Y-axis direction, and a pipetting manipulator driving assembly arranged on the pipetting manipulator support, the pipetting manipulator driving assembly is drivingly connected to the pipette, and the pipette is provided with a plurality of pipetting heads.

[0013] Further improvement of the above scheme is that the quality inspection module comprises a camera and a cell 3D imager, the camera is provided with a plurality of camera and is arranged on one side of the tissue treatment instrument and the centrifuge respectively, and the cell 3D imager is arranged on the rack and cooperates with the transferring module to complete cell imaging.

[0014] The utility model has the advantages that the utility model discloses a rack, a workbench arranged on the rack, a transferring module, a tissue crushing module, a centrifugal module and a quality inspection module, the workbench is used for storing consumables and providing a working area for experimental operation, the tissue crushing module is used for cutting, grinding and digesting sample tissues to obtain single cell suspension, the centrifugal module is used for high-purity separation, concentration and refining of sample solution to obtain single cell suspension meeting requirements, the quality inspection module is used for detecting whether tissue treatment is completed, and the transferring module is used for transferring sample and treated single cell suspension between the tissue crushing module, the centrifugal module and the quality inspection module.

[0015] The utility model discloses a transfer module is with the mutual cooperation of tissue breaking module, centrifugal module, quality inspection module, and the transfer module completes the operation of clamping pipe, uncovering, clamping plate, pipetting, and the tissue breaking module is used for cutting, grinding, digesting sample tissue to obtain single cell suspension, and the centrifugal module is used for high-purity separation, concentration, refining to sample solution to obtain the single cell suspension that meets the demand, and the quality inspection module is used for judging whether tissue processing is completed, can complete the cell extraction process before organoid culture completely automation, obtains corresponding organoid single cell suspension, and does not need manual operation intervention, reduces the experimental error, and simultaneously, various module cooperation works, idle module can alternate job completion, can effectively be applicable to large quantities of job, not only improves work efficiency, simultaneously replaces manual operation to guarantee stability. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 It is the structure schematic diagram of the utility model.

[0017] Figure 2 It is the structure schematic diagram of the utility model's clamping pipe transfer device.

[0018] Figure 3 It is the structure schematic diagram of the utility model's multi-shaft clamping plate mechanical hand subassembly.

[0019] Figure 4 It is the structure schematic diagram of the utility model's transfer plate transfer subassembly.

[0020] Figure 5 It is the structure schematic diagram of the utility model's pipetting device.

[0021] Figure 6 It is the structure schematic diagram of the utility model's tissue processing instrument.

[0022] Figure 7 It is the contrastive drawing of the utility model's automatic organoid cell extraction cell acquisition rate and artificial processing.

[0023] Figure 8 It is the contrastive drawing of the utility model's automatic organoid cell extraction cell harvest amount and artificial processing.

[0024] Figure 9 It is the contrastive drawing of the utility model's automatic organoid cell extraction cell average number and artificial processing.

[0025] Figure 10 It is the contrastive drawing of the utility model's automatic organoid cell extraction cell average number and artificial processing.

[0026] Explanation of reference signs: rack 1, workbench 11, transfer module 12, tissue breaking module 13, tissue processing instrument 131, tissue processing tube 1311, tissue processing channel 1312, heating sleeve 1313, centrifugal module 14, centrifuge 141, quality inspection module 15, camera 151, cell 3D imager 152, pipette tip transfer device 2, XYZ axis motion driving assembly 21, pipette tip assembly support seat 22, pipette tip assembly 23, rotation driving assembly 231, rotation seat 232, pipette tip driving assembly 233, pipette tip claw 234, anti-skid line 235, clamp plate transfer device 3, multi-axis clamp plate manipulator assembly 31, X-axis sliding rail 311, X-axis driving assembly 312, Y-axis sliding support seat 313, Y-axis driving assembly 314, clamp plate holder assembly 315, clamp plate holder support seat 3151, clamp plate holder sliding seat 3152, clamp plate holder sliding driving assembly 3153, clamp plate holder rotation seat 3154, clamp plate holder rotation driving assembly 3155, L-shaped clamp jaw 3156, L-shaped clamp jaw driving assembly 3157, rotating plate transfer assembly 32, rotating plate transfer sliding rail 321, rotating plate transfer sliding seat 322, rotating plate transfer sliding driving assembly 323, Z-axis sliding arm 324, Z-axis sliding arm driving assembly 325, rotating plate holder rotation driving assembly 326, rotating plate holder assembly 327, rotating plate clamp jaw support seat 3271, rotating plate clamp jaw 3272, rotating plate clamp jaw driving assembly 3273. DETAILED DESCRIPTION

[0027] The utility model will be further explained in connection with the drawings, such as Figures 1-10 The utility model discloses a primary cell automatic extraction device, including rack 1, set up on the workbench 11 of rack 1, transfer module 12, tissue breaking module 13, centrifugal module 14, quality inspection module 15, workbench 11 is used to store consumables and provides work area for experimental operation, tissue breaking module 13 is used for cutting, grinding, digesting sample tissue to obtain single cell suspension, centrifugal module 14 is used for high-purity separation, concentration, refining to obtain the single cell suspension that meets the demand of sample solution, and quality inspection module 15 is used to judge whether tissue processing is completed, the tissue sample to be handled is sent into tissue breaking module 13 to cut, grind, digest sample tissue by transfer module 12, then obtains single cell suspension by centrifugal module 14 and judges whether tissue processing is handled fully by quality inspection module 15.

[0028] The utility model discloses tissue breaking module 13 including setting on the tissue processing instrument 131 of rack 1, centrifugal module 14 includes setting on the centrifuge 141 of rack 1, and tissue processing instrument 131 completes the work of tissue shearing, digestion, and centrifuge 141 is centrifuged to the solution handled to obtain single cell suspension.

[0029] The utility model discloses tissue processing instrument 131 including tissue processing pipe 1311, tissue processing channel 1312, heating cover 1313, tissue processing pipe 1311 is used to hold the tissue of processing and is used to handle the tissue lysis solution and / or enzyme of tissue, tissue processing channel 1312 is used to install tissue processing pipe 1311, heating cover 1313 is used for heating tissue processing pipe 1311, and the sample of the tissue of processing is placed into tissue processing pipe 1311 by transfer module 12, and the heating cover 1313 can heat treatment to tissue processing pipe, thereby accelerate the speed of tissue processing reaction, and also can heat treatment to the tissue processing environment of temperature demand, suitable for different sample tissue processing occasion, the utility model discloses tissue processing channel 1312 is provided with a plurality of, and a plurality of tissue processing channels 1312 can place a plurality of tissue processing pipes 1311, and a plurality of sample tissues are handled, and the work efficiency is effectively improved.

[0030] The utility model discloses transfer system 201 includes pinch pipe transfer device 2, be used for clamping micro -hole plate's clamp plate transfer device 3, be used for sucking and transfer reagent solution's pipette device 4, pinch pipe transfer device 2 is used to finish reagent pipe clamping and transfer and / or uncapping operation between pretreatment module 18 and automatic culture module 19, clamp plate transfer device 3 is used to finish clamping micro -hole plate's operation between drug sensitive detection module 20, automatic culture module 19 and worktable 17, pipette device 4 is used to finish pipetting operation between automatic culture module 19, drug sensitive detection module 20 and worktable 17, compared with the prior organoid workstation, only to handle device or system simple stacking placement, still need staff to cooperate operation, handle equipment or system's sudden situation simultaneously, cannot realize the automation of true meaning, and the utility model discloses transfer system transfer system 201 is divided into pinch pipe transfer device 2, clamp plate transfer device 3, pipette device 4, can finish a series of artificial operations such as organoid culture to drug sensitive detection inside transfer micro -hole plate, transfer reagent pipe, pipetting, drop liquid, it replaces all artificial operations that the equipment in organoid workstation cannot finish, and the equipment module of this workstation can completely realize the complete automation of organoid from culture to drug sensitive detection process, the utility model discloses workstation different device division of labor cooperation each module in the workstation, not only can efficient, fast finish a series of work such as organoid from culture to drug sensitive detection, effectively avoid the high failure rate and instability of artificial operation, and different device division of labor does not influence each other, and single device failure will not affect the work of another device, improve the work efficiency while ensuring stability, compared with the low efficiency of artificial operation experiment, can be further applicable to the batch organoid culture and drug sensitive detection work occasion of large batch, large output.

[0031] The utility model discloses clamp pipe transfer device 2 includes setting on the XYZ axle motion drive assembly 21 of rack 1, clamp pipe subassembly support seat 22, the rotation setting of clamp pipe subassembly 23 on the support seat, XYZ axle motion drive assembly 21 drive connection clamp pipe subassembly support seat 22, clamp pipe subassembly 23 includes the rotation drive assembly 231 of rotation setting on the support seat, the rotation seat 232 of rotation connection in the power output end of rotation drive assembly 231, the clamp pipe drive assembly 233 of setting on the rotation seat 232, the clamp pipe claw 234 of sliding connection on the rotation seat 232 along the horizontal direction, clamp pipe drive assembly 233 drive connection clamp pipe claw 234, still be provided with antiskid line 235 on the clamp pipe claw 234, clamp pipe transfer device 2 completes the clamp of reagent pipe between each module and removes the operation of sending, and still be provided with antiskid line 235 on the clamp pipe claw 234, compared with ordinary mechanical hand, the setting of antiskid line 235 can guarantee that the reagent pipe of clamp does not have the problem of slipping, and also can complete the operation of reagent pipe opening, closing, can better be applicable to the work flow of organ related.

[0032] The utility model discloses clamp plate transfer device 3 includes multiaxis clamp plate mechanical hand subassembly 31, multiaxis clamp plate mechanical hand subassembly 31 includes setting on the X axle slide rail 311 of rack 1, X axle drive subassembly 312, along the X axle direction sliding setting on the Y axle sliding support seat 313 of X axle slide rail 311, Y axle drive subassembly 314, along the Y axle direction sliding setting on the clamp plate toshand subassembly 315 of Y axle sliding support seat 313, X axle drive subassembly 312 drive connection Y axle sliding support seat 313, Y axle drive subassembly 314 drive connection clamp plate toshand subassembly 315, clamp plate toshand subassembly 315 includes clamp plate toshand support seat 3151, along the Y axle direction sliding setting on the clamp plate toshand support seat 3151 on the clamp plate toshand sliding seat 3152, setting on the clamp plate toshand support seat 3151 on the clamp plate toshand sliding drive subassembly 3153, along the horizontal direction rotation setting on the clamp plate toshand sliding seat 3152 on the clamp plate toshand rotation seat 3154, setting on the clamp plate toshand sliding seat 3152 on the clamp plate toshand rotation drive subassembly 3155, two along the X axle direction opposite sliding setting on the clamp plate toshand sliding seat 3152 on the L shape clamping jaw 3156, setting on the clamp plate toshand rotation seat 3154 on the L shape clamping jaw drive subassembly 3157, clamp plate toshand sliding drive subassembly 3153 drive connection clamp plate toshand sliding seat 3152, clamp plate toshand rotation drive subassembly 3155 drive connection clamp plate toshand rotation seat 3154, L shape clamping jaw drive subassembly 3157 drive connection L shape clamping jaw 3156, and L shape clamping jaw 3156 can hold up microporous board also can be opposite L shape clamping jaw 3156 from the collection to hold microporous board, complete the conveyance between each module microporous board or board material.

[0033] The clamping plate conveying device 3 further comprises a rotating plate conveying assembly 32 arranged on the top of the rack, the rotating plate conveying assembly 32 comprises a rotating plate conveying sliding rail 321 arranged on the rack, a rotating plate conveying sliding seat 322 slidingly connected to the rotating plate conveying sliding rail 321, a rotating plate conveying sliding driving assembly 323 arranged on the rotating plate conveying sliding rail 321, a Z-axis sliding arm 324 slidingly connected to the rotating plate conveying sliding rail 321 along the Z-axis direction, a Z-axis sliding arm driving assembly 325 arranged on the rotating plate conveying sliding seat 322, a rotating plate supporting hand assembly 327 rotationally connected to the bottom of the Z-axis sliding arm 324, and a rotating plate supporting hand rotating driving assembly 326 arranged on the Z-axis sliding arm 324, the rotating plate conveying sliding driving assembly 323 is drivingly connected to the rotating plate conveying sliding seat 322, the Z-axis sliding arm driving assembly 325 is drivingly connected to the Z-axis sliding arm 324, and the rotating plate supporting hand rotating driving assembly 326 drives the rotating plate supporting hand assembly 327 to rotate.

[0034] The utility model pipette device 4 includes setting in the rack 1 on the pipette mechanical hand slide rail 41 along the X axle direction, the pipette mechanical hand slide rail 41 on the pipette mechanical hand slide seat 42 of sliding setting along the X axle direction, the pipette mechanical hand support seat 43 of setting on the pipette mechanical hand slide seat 42, the pipette 44 of sliding setting on the pipette support seat along the Y axle direction, the pipette mechanical hand drive assembly 45 of setting on the pipette mechanical hand support seat 43, the pipette mechanical hand drive assembly 45 drive connection pipette 44, and pipette 44 is provided with a plurality of pipette heads 441;Pipette mechanical hand cooperation pipette 44 can complete the operation that needs to move and send solution, reagent in the process of organoid culture to drug sensitivity detection, and pipette 44 is provided with a plurality of pipette heads 441, and simultaneously completes a plurality of pipette operations, effectively improves work efficiency, and can be used for pipetting different reagents, avoids mutual cross infection, and does not need to change pipette head 141, relative to the traditional staff operation pipette, can be better applied to the working situation that needs to frequently pipette and change liquid in organoid culture, drug sensitivity detection and the like.

[0035] The utility model quality inspection module 15 includes camera 151, cell 3D imaging appearance 152;Camera 151 is provided with a plurality of and respectively sets up in one side of tissue processing appearance 131 and centrifuge 141, and cell 3D imaging appearance 152 sets up on the rack 1 and moves and sends module 12 cooperation and completes cell imaging to judge whether tissue processing is completed.

[0036] Working principle:

[0037] The utility model through moving and sending module 12 will be mutually matched with tissue crushing module 13, centrifugal module 14, quality inspection module 15, and moving and sending module 12 completes the operation of clamping pipe, opening cover, clamping plate, pipetting, tissue crushing module 13 cuts, grinds, digests sample tissue to obtain single cell suspension, centrifugal module 14 carries out high-purity separation, concentration, refining to sample solution to obtain the single cell suspension that meets the demand, and quality inspection module 15 judges whether tissue processing is completed, can completely automatically complete the cell extraction process before organoid culture, obtains the corresponding organoid single cell suspension, as shown in the drawing, Figures 7-10 As shown, 10 tissue samples are prepared, each sample is divided into two equal parts, and artificial digestion treatment and automatic sample treatment are carried out respectively, and the cell yield of 10 samples is counted, and after 7 days of culture, the average value of the number of organoids and the diameter of organoids is detected, so that the automatic extraction device of the utility model does not need manual operation intervention, the yield of organoid cells is much higher than that of manual extraction, the experimental error is reduced, and each module works cooperatively, so that the idle module can alternately complete the work, which can effectively be applied to large quantities of work, improves the work efficiency, and replaces manual operation to ensure stability.

[0038] Of course, the above only the preferred embodiments of the present application, therefore, the structure, features and principles of the present application described in the patent application range of equivalent changes or modifications made to the present application, are included in the patent application range.

Claims

1. An automated primary cell extraction device, characterized in that: The device comprises a rack (1), a workbench (11) arranged on the rack (1), a transfer module (12), a tissue breaking module (13), a centrifugal module (14) and a quality inspection module (15); the workbench (11) is used for storing consumables and providing a working area for experimental operation; the tissue breaking module (13) is used for cutting, grinding and digesting sample tissues to obtain a single-cell suspension; the centrifugal module (14) is used for high-purity separation, concentration and refinement of sample solutions to obtain a single-cell suspension meeting requirements; the quality inspection module (15) is used for detecting whether the tissue processing is completed; and the transfer module (12) is used for transferring samples and processed single-cell suspensions among the tissue breaking module (13), the centrifugal module (14) and the quality inspection module (15).

2. A primary cell automated extraction apparatus as defined in claim 1, wherein: The tissue breaking module (13) comprises a tissue processor (131) arranged on the rack (1), and the centrifugal module (14) comprises a centrifugal machine (141) arranged on the rack (1).

3. A primary cell automated extraction apparatus as defined in claim 2, wherein: The tissue processor (131) comprises a tissue processing tube (1311), a tissue processing channel (1312) and a heating jacket (1313); the tissue processing tube (1311) is used for containing tissues to be processed and tissue lysates and / or enzymes used for processing the tissues; the tissue processing channel (1312) is used for mounting the tissue processing tube (1311); and the heating jacket (1313) is used for heating the tissue processing tube (1311).

4. A primary cell automated extraction apparatus as defined in claim 3, wherein: The tissue processing channel (1312) is provided with a plurality of tissue processing tubes (1311).

5. The primary cell automated extraction apparatus of claim 1, wherein: The transfer module (12) comprises a pipette clamping and transferring device (2), a clamping plate transferring device (3) and a pipetting device (4); the pipette clamping and transferring device (2) is used for clamping and opening a cap; the clamping plate transferring device (3) is used for transporting a micro-hole plate; and the pipetting device (4) is used for pipetting.

6. A primary cell automated extraction apparatus as defined in claim 5, wherein: The pipette clamping and transferring device (2) comprises an XYZ-axis motion driving assembly (21) arranged on the rack (1), a pipette clamping assembly support seat (22) and a pipette clamping assembly (23) rotatably arranged on the pipette clamping assembly support seat (22); the XYZ-axis motion driving assembly (21) is drivingly connected to the pipette clamping assembly support seat (22); the pipette clamping assembly (23) comprises a rotation driving assembly (231) rotatably arranged on the pipette clamping assembly support seat (22), a rotation seat (232) rotationally connected to a power output end of the rotation driving assembly (231), a pipette clamping driving assembly (233) arranged on the rotation seat (232) and a pipette clamping jaw (234) slidingly connected to the rotation seat (232) in a horizontal direction; the pipette clamping driving assembly (233) is drivingly connected to the pipette clamping jaw (234); and an anti-skid pattern (235) is further arranged on the pipette clamping jaw (234).

7. A primary cell automated extraction apparatus as defined in claim 5, wherein: The clamp plate conveying device (3) comprises a multi-axis clamp plate manipulator assembly (31), the multi-axis clamp plate manipulator assembly (31) comprises an X-axis sliding rail (311) arranged on the rack (1), an X-axis driving assembly (312), a Y-axis sliding support seat (313) arranged on the X-axis sliding rail (311) and sliding in the X-axis direction, a Y-axis driving assembly (314), a clamp plate supporting hand assembly (315) arranged on the Y-axis sliding support seat (313) and sliding in the Y-axis direction, the X-axis driving assembly (312) is drivingly connected with the Y-axis sliding support seat (313), and the Y-axis driving assembly (314) is drivingly connected with the clamp plate supporting hand assembly (315); the clamp plate supporting hand assembly (315) comprises a clamp plate supporting hand support seat (3151), a clamp plate supporting hand sliding seat (3152) arranged on the clamp plate supporting hand support seat (3151) and sliding in the Y-axis direction, a clamp plate supporting hand sliding driving assembly (3153) arranged on the clamp plate supporting hand support seat (3151), a clamp plate supporting hand rotating seat (3154) arranged on the clamp plate supporting hand sliding seat (3152) and rotating in the horizontal direction, a clamp plate supporting hand rotating driving assembly (3155) arranged on the clamp plate supporting hand sliding seat (3152), two L-shaped clamping jaws (3156) arranged on the clamp plate supporting hand sliding seat (3152) and sliding in the X-axis direction, and an L-shaped clamping jaw driving assembly (3157) arranged on the clamp plate supporting hand rotating seat (3154); the clamp plate supporting hand sliding driving assembly (3153) is drivingly connected with the clamp plate supporting hand sliding seat (3152), the clamp plate supporting hand rotating driving assembly (3155) is drivingly connected with the clamp plate supporting hand rotating seat (3154), and the L-shaped clamping jaw driving assembly (3157) is drivingly connected with the L-shaped clamping jaw (3156).

8. A primary cell automated extraction apparatus as claimed in claim 7, wherein: The clamp plate conveying device (3) further comprises a rotating plate conveying assembly (32) arranged on the top of the rack (1), the rotating plate conveying assembly (32) comprises a rotating plate conveying sliding rail (321) arranged on the rack (1), a rotating plate conveying sliding seat (322) slidingly connected to the rotating plate conveying sliding rail (321), a rotating plate conveying sliding driving assembly (323) arranged on the rotating plate conveying sliding rail (321), a Z-axis sliding arm (324) slidingly connected to the rotating plate conveying sliding rail (321) along the Z-axis direction, a Z-axis sliding arm driving assembly (325) arranged on the rotating plate conveying sliding seat (322), a rotating plate supporting hand assembly (327) rotationally connected to the bottom of the Z-axis sliding arm (324), and a rotating plate supporting hand rotating driving assembly (326) arranged on the Z-axis sliding arm (324), the rotating plate conveying sliding driving assembly (323) is drivingly connected to the rotating plate conveying sliding seat (322), the Z-axis sliding arm driving assembly (325) is drivingly connected to the Z-axis sliding arm (324), and the rotating plate supporting hand rotating driving assembly (326) drives the rotating plate supporting hand assembly (327) to rotate; the rotating plate supporting hand assembly (327) comprises a rotating plate clamp jaw supporting seat (3271) arranged on the bottom of the Z-axis sliding arm (324), two rotating plate clamp jaws (3272) slidingly arranged on the rotating plate clamp jaw supporting seat (3271) in the X-axis direction, and a rotating plate clamp jaw driving assembly (3273) arranged on the rotating plate clamp jaw supporting seat (3271), and the rotating plate clamp jaw driving assembly (3273) drives the two rotating plate clamp jaws (3272) to move in the X-axis direction.

9. The primary cell automated extraction apparatus of claim 5, wherein: The pipetting device (4) comprises a pipetting manipulator sliding rail (41) arranged on the rack (1) in the X-axis direction, a pipetting manipulator sliding seat (42) slidingly arranged on the pipetting manipulator sliding rail (41) in the X-axis direction, a pipetting manipulator supporting seat (43) arranged on the pipetting manipulator sliding seat (42), a pipette (44) slidingly arranged on the pipetting supporting seat in the Y-axis direction, and a pipetting manipulator driving assembly (45) arranged on the pipetting manipulator supporting seat (43), the pipetting manipulator driving assembly (45) is drivingly connected to the pipette (44), and the pipette (44) is provided with a plurality of pipetting heads (441).

10. The primary cell automated extraction apparatus of claim 2, wherein: The quality inspection module (15) comprises a camera (151) and a cell 3D imager (152), the camera (151) is arranged on one side of the tissue treatment instrument (131) and the centrifugal machine (141) and is provided in plurality, the cell 3D imager (152) is arranged on the rack (1) and cooperates with the conveying module (12) to complete cell imaging.

Images