Recombinant protein batch chromatography detection equipment

Abstract

The utility model relates to the technical field of recombinant protein chromatography detection, in particular to recombinant protein batch chromatography detection equipment. A batch chromatography detection mechanism composed of a material tank, a material injection pump, a batch conveying pipe frame, a supporting plate, a sealing cover, a chromatographic column, a UV detector and a collecting tank is jointly mounted among a placement seat, a cross-shaped supporting plate frame I, a cross-shaped supporting plate frame II and a support; according to the present invention, the three chromatographic columns are arranged, such that the equipment can simultaneously perform separation detection on the recombinant protein in the three chromatographic columns during the one-time separation and purification process so as to effectively reduce the separation and purification time of the batch recombinant protein; the large-batch conveying pipe frame is communicated with the chromatographic columns on the different branch plates on the second cross-shaped supporting plate frame, it can be guaranteed that one set of chromatographic columns can be continuously used when the other set of chromatographic columns are blocked, and therefore the continuity of the large-batch recombinant protein separation and purification process is guaranteed, and the large-batch recombinant protein separation and purification efficiency is higher.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of recombinant protein chromatography detection, in particular to a batch recombinant protein chromatography detection device. BACKGROUND

[0002] Recombinant protein is a foreign protein expressed in host cells through genetic engineering technology. This technology enables scientists to mass-produce specific proteins in the laboratory or industrial production for research, treatment or industrial applications, wherein a chromatography detection device is required to separate and purify the recombinant protein during the preparation of the recombinant protein.

[0003] The existing recombinant protein chromatography detection device mainly comprises a mounting frame, a tank mounted on the mounting frame, a pump mounted at the liquid outlet of the tank, a single chromatography column mounted on the mounting frame, a UV detector arranged below the chromatography column, and a collection container arranged below the UV detector. During chromatography purification of the recombinant protein, the recombinant protein mixture in the tank is first pumped into the chromatography column under the action of the pump, so that the target recombinant protein is separated after the recombinant protein mixture flows through the filter gel in the chromatography column. The separated target recombinant protein is finally collected in the collection container after passing through the UV detector, thereby realizing the separation and purification of the recombinant protein.

[0004] Although the existing recombinant protein chromatography detection device can separate and purify the large amount of target protein after cultivation, the use of a single chromatography column for chromatography purification of a large amount of target protein will result in a long separation and purification time of the target protein. In addition, after a long time of continuous chromatography, it takes a long time to replace the clogged chromatography column, which results in discontinuity in the separation and purification process of the large amount of recombinant protein, thereby reducing the separation and purification efficiency of the large amount of recombinant protein. SUMMARY

[0005] In view of the deficiencies of the prior art, the present application aims to provide a batch recombinant protein chromatography detection device which can simultaneously separate and detect the recombinant protein in three chromatography columns in one separation and purification process, and can use lifting and rotating adjustment to put another group of chromatography columns into use when one group of chromatography columns is clogged, thereby ensuring the continuity of the recombinant protein detection process and reducing the separation and purification time of the large amount of recombinant protein.

[0006] The above application object of the present application is achieved by the following technical scheme:

[0007] The utility model provides a kind of batch chromatography detection equipment of recombinant protein, including support, the middle part of the top of support is equipped with mounting sleeve, the outer middle part of mounting sleeve is equipped with cross support plate frame one, the outer upper part of mounting sleeve is equipped with cross support plate frame two, the bottom of support is equipped with rotating mechanism, and rotating mechanism includes rotating motor and rotating shaft, rotating motor is installed in the middle part of the bottom of support, and rotating shaft is connected in the power output end of rotating motor;Rotating shaft upper end middle part's self-owned inner recess structure is also equipped with electric telescopic rod, and the telescopic part of electric telescopic rod is connected with placing seat, and batch chromatography detection mechanism is jointly installed on placing seat, cross support plate frame one, cross support plate frame two and support, and batch chromatography detection mechanism includes tank, injection pump, batch conveying pipe frame, support plate, sealing cover, chromatographic column, UV detector and collection tank, tank is installed on placing seat, support plate is installed on the one side wall outside placing seat, injection pump is installed on the upper side of support plate near tank, batch conveying pipe frame is installed in the middle part of support plate, and batch conveying pipe frame is provided with three branch pipes, and each branch pipe is connected with a sealing cover in lower side, chromatographic column is installed on the branch plate of cross support plate frame two, and there are three chromatographic columns on each branch plate of cross support plate frame two, UV detector is installed on the branch plate of cross support plate frame one, and collection tank is installed on the upper side of support.

[0008] Optionally, the batch conveying pipe frame is composed of a conveying main pipe and three branch pipes installed on the lower side of the conveying main pipe.

[0009] Optionally, the cross support plate frame two is composed of a circular ring fixedly connected with the mounting sleeve and four branch plates installed in a ring shape on the periphery of the circular ring.

[0010] Optionally, the mounting sleeve is welded with the support, and the rotating shaft penetrates through the mounting sleeve and is rotationally connected with the mounting sleeve.

[0011] Optionally, the fixed part of the electric telescopic rod is bolted with the reserved groove at the top end of the rotating shaft, and the telescopic part of the electric telescopic rod is bolted with the placing seat.

[0012] Optionally, the injection pump is bolted with the support plate, and the injection pump is connected with the tank and the batch conveying pipe frame through pipelines.

[0013] Optionally, the sealing cover is matched in size with the opening size at the top end of the chromatographic column, and the branch pipes at the bottom end of the batch conveying pipe frame penetrate through the sealing cover.

[0014] Optionally, each branch plate on the cross support frame two is further provided with a linkage clamping assembly for clamping the chromatographic column, the linkage clamping assembly comprises a mounting slot, a clamping plate, a bidirectional threaded part, a guide slot, a rotating rod and a hand wheel.

[0015] Optionally, three mounting slots are arranged on each branch plate on the cross support frame one, two clamping plates are symmetrically arranged in each mounting slot, the guide slots are arranged on the cross support frame two at the two side matching parts of the clamping plates, the rotating rod is arranged in one of the guide slots, the bidirectional threaded part is reserved on the rotating rod at the two clamping plate matching parts in each mounting slot, and the hand wheel is connected to the exposed end of the rotating rod.

[0016] In conclusion, the present application has at least one of the following beneficial technical effects:

[0017] The batch chromatographic detection mechanism composed of the material tank, the material injection pump, the batch conveying pipe frame, the branch plate, the sealing cover, the chromatographic column, the UV detector and the collection tank is arranged between the placing seat, the cross support frame one and the cross support frame two, so that the recombination protein in the three chromatographic columns can be separated and detected simultaneously in one separation and purification process, the separation and purification time of the batch recombination protein is effectively reduced, the chromatographic column on the different branch plates on the batch conveying pipe frame and the cross support frame two is communicated under the action of the rotating mechanism and the electric telescopic rod, the other chromatographic column can continue to be used when one chromatographic column is blocked, the continuity of the large batch recombination protein separation and purification process is ensured, and the separation and purification efficiency of the large batch recombination protein is higher. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 is a structural schematic view provided by the embodiment of the present application;

[0019] Figure 2 is a main sectional view provided by the embodiment of the present application;

[0020] Figure 3 is a top view of the cross support frame two provided by the embodiment of the present application;

[0021] Figure 4 is a structural schematic view of the clamping plate provided by the embodiment of the present application; Figure 3 is an enlarged view of A in the middle;

[0022] Figure 5 is a structural schematic view of the clamping plate provided by the embodiment of the present application;

[0023] Explanation of reference signs: 1, placing seat; 2, electric telescopic rod; 3, cross support plate frame two; 4, cross support plate frame one; 5, support; 6, batch chromatography detection mechanism; 61, material tank; 62, material injection pump; 63, batch conveying pipe frame; 64, sealing cover; 65, chromatography column; 66, UV detector; 67, collection tank; 68, support plate; 7, rotating mechanism; 71, rotating motor; 72, rotating shaft; 8, linkage clamping assembly; 81, mounting groove; 82, clamping plate; 83, bidirectional threaded part; 84, guide groove; 85, rotating rod; 86, hand wheel; 9, mounting sleeve. DETAILED DESCRIPTION

[0024] The present application will be further described in detail below with reference to the accompanying drawings.

[0025] In order to more clearly understand the technical solutions shown in the embodiments of the present application, first, the structure and working principle of the existing recombinant protein chromatography detection equipment are introduced.

[0026] The existing recombinant protein chromatography detection equipment mainly consists of a mounting frame, a material tank mounted on the mounting frame, a suction pump mounted at the liquid outlet of the material pipe, a single chromatography column mounted on the mounting frame, a UV detector arranged below the chromatography column, and a collection container arranged below the UV detector. When chromatography purification of recombinant protein is performed, the recombinant protein mixed solution in the material tank is first sucked into the chromatography column under the action of the suction pump, so as to realize separation of the target recombinant protein after the recombinant protein mixed solution flows through the filter gel in the chromatography column. The separated target recombinant protein is finally dropped into the collection container after being detected by the UV detector, thereby realizing separation and purification of the recombinant protein.

[0027] Please refer to Figures 1-3The application discloses a batch chromatography detection equipment for a recombinant protein, which comprises a support 5, a mounting sleeve 9 is arranged at the middle of the top of the support 5, a cross support plate rack I 4 is arranged at the outer middle of the mounting sleeve 9, a cross support plate rack II 3 is arranged at the outer upper portion of the mounting sleeve 9, a rotating mechanism 7 is arranged at the bottom of the support 5, the rotating mechanism 7 comprises a rotating motor 71 and a rotating shaft 72, the rotating motor 71 is arranged at the middle of the bottom of the support 5, the rotating shaft 72 is connected to the power output end of the rotating motor 71, an electric telescopic rod 2 is further arranged at the inner recess structure of the middle of the upper end of the rotating shaft 72, a placing seat 1 is connected to the telescopic part of the electric telescopic rod 2, a batch chromatography detection mechanism 6 is arranged on the placing seat 1, the cross support plate rack I 4, the cross support plate rack II 3 and the support 5, the batch chromatography detection mechanism 6 comprises a material tank 61, a material injection pump 62, a batch conveying pipe rack 63, a support plate 68, a sealing cover 64, a chromatography column 65, a UV detector 66 and a collection tank 67, the material tank 61 is arranged on the placing seat 1, the support plate 68 is arranged on the outer side wall of the placing seat 1, the material injection pump 62 is arranged on the upper side of the support plate 68 and close to the material tank 61, the batch conveying pipe rack 63 is arranged at the middle of the support plate 68, the batch conveying pipe rack 63 is provided with three branch pipes, each branch pipe is connected with one sealing cover 64 at the lower side, the chromatography column 65 is arranged on the branch plate 68 of the cross support plate rack II 3, and there are three chromatography columns 65 on each branch plate 68 of the cross support plate rack II 3, the UV detector 66 is arranged on the branch plate 68 of the cross support plate rack I 4, and the collection tank 67 is arranged on the upper side of the support 5.

[0028] Specifically, when a large batch of recombinant proteins is separated and purified, corresponding separation gel is first added into the chromatography column 65, then the batch conveying pipe rack 63 is moved downwards under the action of the electric telescopic rod 2, so that the sealing cover 64 is inserted into the corresponding chromatography column 65, then the recombinant protein mixed solution in the material tank 61 is conveyed into the chromatography column 65 under the action of the material injection pump 62, so that the target protein in the recombinant protein mixed solution is separated when the recombinant protein mixed solution passes through the chromatography column 65, the separated target protein is collected into the collection tank 67 after being detected by the UV detector 66, and when a group of chromatography columns 65 are blocked, the batch conveying pipe rack 63 can be first moved upwards under the action of the electric telescopic rod 2, and meanwhile the batch conveying pipe rack 63 is moved to above another group of chromatography columns 65 under the action of the rotating motor 71 and the rotating shaft 72, and then the above chromatography detection operation is repeated, so that the large batch of recombinant proteins can be continuously separated and purified.

[0029] Please refer to Figures 1-2 The batch conveying pipe rack 63 is composed of a conveying main pipe and three branch pipes arranged at the lower side of the conveying main pipe.

[0030] As an implementation, the three branch pipes enable the batch delivery pipe rack 63 to simultaneously deliver the band separation and purification of the recombinant protein mixture to the three chromatographic columns 65.

[0031] Please refer to Figures 1-2 The cross support rack 2 is composed of a circular ring fixedly connected with the mounting sleeve and four branch plates 68 installed in a ring shape on the periphery of the circular ring.

[0032] As an implementation, the design of the four branch plates 68 enables the cross support rack 1 to simultaneously install four groups of chromatographic columns 65, thereby ensuring the alternate use of the four groups of chromatographic columns 65.

[0033] Please refer to Figures 1-2 The mounting sleeve 9 is welded with the support 5, and the rotating shaft 72 penetrates through the mounting sleeve 9 and is rotationally connected with the mounting sleeve 9.

[0034] As an implementation, the mounting sleeve 9 is mainly used to provide a mounting base for the cross support rack 1 and the cross support rack 2, thereby ensuring the reliable fixation of the cross support rack 1 and the cross support rack 2. Meanwhile, the mounting sleeve 9 also increases the structural strength of the rotating shaft 72 during rotation.

[0035] Please refer to Figures 1-2 The fixed part of the electric telescopic rod 2 is bolted with the groove reserved at the top end of the rotating shaft 72, and the telescopic part of the electric telescopic rod 2 is bolted with the placing seat 1.

[0036] As an implementation, the electric telescopic rod 2 is mainly used to synchronously adjust the height of the batch delivery pipe rack 63, the material injection pump 62, and the material tank 61, so as to conveniently lower the batch delivery pipe rack 63 after the rotation of the placing seat 1.

[0037] Please refer to Figures 1-2 The material injection pump 62 is bolted with the support plate 68, and the material injection pump 62 is connected with the material tank 61 and the batch delivery pipe rack 63 through pipelines.

[0038] As an implementation, the material tank 61 is mainly used to store the recombinant protein mixture to be separated and purified, and the material injection pump 62 is mainly used to deliver the recombinant protein mixture in the material tank 61 to the batch delivery pipe rack 63.

[0039] Please refer to Figures 1-2 The sealing cover 64 is matched in size with the opening at the top end of the chromatographic column 65, and the branch pipe at the bottom end of the batch delivery pipe rack 63 penetrates through the sealing cover 64.

[0040] As an implementation form, the sealing cover 64 is matched in size with the opening at the top end of the chromatographic column 65, so as to ensure reliable sealing of the chromatographic column 65 by the sealing cover 64, and the branch pipe of the bottom end of the batch delivery pipe rack 63 is penetrated through the sealing cover 64, so as to ensure convenient delivery of the recombinant protein mixture in the batch delivery pipe rack 63 to the chromatographic column 65.

[0041] Please refer to Figures 1-4 , each branch plate 68 on the cross support plate rack two 3 is further provided with a linkage clamping assembly 8 for clamping the chromatographic column 65, and the linkage clamping assembly 8 comprises a mounting groove 81, a clamping plate 82, a bidirectional threaded portion 83, a guide groove 84, a rotating rod 85 and a hand wheel 86.

[0042] As an implementation form, after the chromatographic column 65 is placed between the clamping plates 82 in the mounting groove 81, rotating the hand wheel 86 will cause the two clamping plates 82 to move close to the outer wall of the chromatographic column 65 under the action of threaded transmission, so as to realize stable clamping of the chromatographic column 65.

[0043] Please refer to Figure 2 , Figure 4 and Figure 5 , each branch plate 68 on the cross support plate rack one 4 is provided with three mounting grooves 81, each mounting groove 81 is symmetrically provided with two clamping plates 82, the cross support plate rack two 3 is provided with a guide groove 84 at the two side matching parts of the clamping plate 82, one of the guide grooves 84 is provided with a rotating rod 85, the rotating rod 85 is provided with a bidirectional threaded portion 83 at the matching part with the two clamping plates 82 in each mounting groove 81, and the hand wheel 86 is connected to the exposed end of the rotating rod 85.

[0044] As an implementation form, the bidirectional threaded portion 83 on the rotating rod 85 is threadedly connected with the guide table on the clamping plate 82, so as to cause the clamping plate 82 to conveniently slide in the mounting groove 81 under the action of threaded transmission.

[0045] Specific working principle is, in a large number of recombinant protein separation and purification, is first added in the chromatographic column 65 corresponding separation gel, then under the action of electric telescopic rod 2 makes batch delivery pipe frame 63 down, to ensure that the sealing cover 64 inserted into the corresponding chromatographic column 65, then under the action of injection pump 62 the batch tank 61 of recombinant protein mixture is delivered to the chromatographic column 65 through batch delivery pipe frame 63, in order to realize the separation of target protein in recombinant protein mixture when the recombinant protein mixture through chromatographic column 65, the target protein after separation is detected by UV detector 66 qualified into the collection tank 67 for collection, while in a group of chromatographic column 65 blockage, can first under the action of electric telescopic rod 2 batch delivery pipe frame 63 up, at the same time under the action of rotary motor 71 and rotary shaft 72 batch delivery pipe frame 63 is moved to another group of chromatographic column 65 directly above, then only need to repeat the above chromatographic detection operation, can continue to separate and purify a large number of recombinant protein, because under the action of the chromatographic detection equipment, not only can in a separation and purification process can simultaneously separate and detect the recombinant protein in three chromatographic column 65, effectively reduce the separation and purification time of batch recombinant protein, and under the action of rotary mechanism 7 and electric telescopic rod 2, realize batch delivery pipe frame 63 and different branch plate 68 on cross support plate frame two 3 on chromatographic column 65 according to the needs of communication, can ensure that another group of chromatographic column 65 can continue to use when a group of chromatographic column 65 blockage, so as to ensure the continuity of large quantities of recombinant protein separation and purification process, make the separation and purification efficiency of large quantities of recombinant protein is higher.

[0046] The embodiments of the specific implementation are the preferred embodiments of the present application, not limited by the protection scope of the present application, therefore: any equivalent changes made according to the structure, shape, principle of the present application should be covered within the protection scope of the present application.

Claims

1. A recombinant protein batch chromatography detection apparatus, characterized by: The application relates to a batch chromatography detection device, which comprises a support (5), a mounting sleeve (9) arranged at the middle of the top end of the support (5), a cross support plate frame I (4) arranged at the middle of the outer part of the mounting sleeve (9), a cross support plate frame II (3) arranged at the upper part of the outer part of the mounting sleeve (9), a rotating mechanism (7) arranged at the bottom end of the support (5), a rotating motor (71) arranged at the middle of the bottom end of the support (5), a rotating shaft (72) connected to the power output end of the rotating motor (71), an electric telescopic rod (2) arranged at the middle of the upper end of the rotating shaft (72), a placing seat (1) connected to the telescopic part of the electric telescopic rod (2), a batch chromatography detection mechanism (6) arranged on the placing seat (1), the cross support plate frame I (4), the cross support plate frame II (3) and the support (5), the batch chromatography detection mechanism (6) comprising a material tank (61), a material injection pump (62), a batch conveying pipe frame (63), a support plate (68), a sealing cover (64), a chromatography column (65), a UV detector (66) and a collecting tank (67), the material tank (61) being arranged on the placing seat (1), the support plate (68) being arranged on the outer side wall of the placing seat (1), the material injection pump (62) being arranged on the upper side of the support plate (68) and close to the material tank (61), the batch conveying pipe frame (63) being arranged at the middle of the support plate (68), the batch conveying pipe frame (63) being provided with three branch pipes, each branch pipe being provided with a sealing cover (64) at the lower side, the chromatography column (65) being arranged on the branch plate (68) of the cross support plate frame II (3), three chromatography columns (65) being arranged on each branch plate (68) of the cross support plate frame II (3), the UV detector (66) being arranged on the branch plate (68) of the cross support plate frame I (4), and the collecting tank (67) being arranged on the upper side of the support (5).

2. The recombinant protein batch chromatography detection device according to claim 1, characterized in that: The batch conveying pipe frame (63) is composed of a conveying main pipe and three branch pipes arranged at the lower side of the conveying main pipe.

3. The recombinant protein batch chromatography detection device according to claim 1, wherein: The cross support plate frame II (3) is composed of a circular ring fixedly connected with the mounting sleeve and four branch plates (68) arranged in a ring shape at the periphery of the circular ring.

4. The recombinant protein batch chromatography detection device according to claim 1, wherein: The mounting sleeve (9) is welded with the support (5), and the rotating shaft (72) penetrates through the mounting sleeve (9) and is rotationally connected with the mounting sleeve (9).

5. The recombinant protein batch chromatography detection device according to claim 4, characterized in that: The fixed part of the electric telescopic rod (2) is bolted with the reserved groove at the top end of the rotating shaft (72), and the telescopic part of the electric telescopic rod (2) is bolted with the placing seat (1).

6. The recombinant protein batch chromatography detection device according to claim 1, wherein: The material injection pump (62) is bolted with the support plate (68), and the material injection pump (62) is connected with the material tank (61) and the batch conveying pipe frame (63) through pipelines.

7. The recombinant protein batch chromatography detection device according to claim 1, wherein: The sealing cover (64) is matched with the size of the top opening of the chromatographic column (65), and the branch pipe at the bottom of the batch conveying pipe rack (63) penetrates through the sealing cover (64).

8. The recombinant protein batch chromatography detection device according to claim 1, wherein: A linkage clamping assembly (8) for clamping the chromatographic column (65) is further installed on each branch plate (68) of the cross support plate rack two (3), and the linkage clamping assembly (8) comprises a mounting groove (81), a clamping plate (82), a bidirectional threaded portion (83), a guide groove (84), a rotating rod (85) and a hand wheel (86).

9. The recombinant protein batch chromatography detection device according to claim 8, characterized in that: Three mounting grooves (81) are arranged on each branch plate (68) of the cross support plate rack one (4), two clamping plates (82) are symmetrically arranged in each mounting groove (81), the guide grooves (84) are arranged on the cross support plate rack two (3) and matched with the two sides of the clamping plates (82), the rotating rod (85) is arranged in one of the guide grooves (84), the bidirectional threaded portion (83) is reserved on the rotating rod (85) and matched with the two clamping plates (82) in each mounting groove (81), and the hand wheel (86) is connected to the exposed end of the rotating rod (85).

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