Reaction device

Abstract

The reaction device comprises a high-pressure gas storage module, a first pressure regulating module, a second pressure regulating module, a third pressure regulating module, a cylinder, a reaction bin and a waste liquid bottle, the first output end of the first pressure regulating module is connected with the cylinder, and the high-pressure gas storage module outputs first gas pressure to the cylinder through the first pressure regulating module, so that the cylinder compresses the reaction bin; the second output end of the second pressure regulating module is connected with the reaction bin, and the high-pressure gas storage module outputs second gas pressure into the reaction bin through the second pressure regulating module, so that the reagents in the plurality of synthetic columns fully react under the action of the second gas pressure; the second air pressure is smaller than the first air pressure; the third output end of the third pressure regulating module is connected with the reaction bin, and the high-pressure gas storage module outputs third gas pressure into the reaction bin through the third pressure regulating module, so that the reacted waste liquid in the plurality of synthesis columns flows into the waste liquid bottle under the action of the third gas pressure; wherein the air pressure value of the third air pressure is larger than that of the second air pressure and smaller than that of the first air pressure.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of reaction equipment, and particularly relates to a reaction device. BACKGROUND

[0002] In biochemical reactions, according to different test purposes or according to test cost considerations, micro-amount of reactants are often used for reaction tests. The smaller the amount of liquid is, the more easily the movement of the liquid is affected by the surface tension of the liquid, which leads to that the micro-amount of liquid cannot fully participate in the reaction, and also leads to that the micro-amount of waste liquid after participating in the reaction is difficult to completely discharge and remains in the reaction device. Therefore, in the existing reaction device, there are problems of not being conducive to the full reaction of the micro-amount of liquid and being easy to remain. CONTENT OF THE UTILITY MODEL

[0003] Based on this, the purpose of the present application is to provide a reaction device which can overcome the shortcomings of the prior art.

[0004] In order to achieve the above purpose, the technical scheme adopted by the present application is as follows:

[0005] A reaction device comprises a high-pressure gas storage module, a first pressure regulating module, a second pressure regulating module, a third pressure regulating module, a gas cylinder, a reaction bin and a waste liquid bottle; the reaction bin comprises a plurality of synthesis columns;

[0006] The output end of the high-pressure gas storage module is connected with the first input end of the first pressure regulating module, the second input end of the second pressure regulating module and the third input end of the third pressure regulating module respectively;

[0007] The first output end of the first pressure regulating module is connected with the gas cylinder, and the high-pressure gas storage module outputs a first gas pressure to the gas cylinder via the first pressure regulating module, so that the gas cylinder compresses the reaction bin;

[0008] The second output end of the second pressure regulating module is connected with the reaction bin, and the high-pressure gas storage module outputs a second gas pressure to the reaction bin via the second pressure regulating module, so that the reagents in the plurality of synthesis columns fully react under the action of the second gas pressure; the gas pressure value of the second gas pressure is less than the first gas pressure;

[0009] The third output end of the third pressure regulating module is connected with the reaction bin, and the high-pressure gas storage module outputs a third gas pressure to the reaction bin via the third pressure regulating module, so that the waste liquid after reaction in the plurality of synthesis columns flows into the waste liquid bottle under the action of the third gas pressure; wherein the gas pressure value of the third gas pressure is greater than the second gas pressure, and the gas pressure value of the third gas pressure is less than the first gas pressure.

[0010] As an implementation manner, the first pressure regulating module comprises a first pressure regulating valve and a first two-way valve;

[0011] The output end of the high-pressure gas storage module is connected with the gas cylinder via the first pressure regulating valve and the first two-way valve.

[0012] As an embodiment, the second pressure regulating module comprises a second pressure regulating valve and a second two-way valve.

[0013] The output end of the high-pressure gas storage module is connected with the reaction chamber via the second pressure regulating valve and the second two-way valve.

[0014] As an embodiment, the third pressure regulating module comprises a third pressure regulating valve and a third two-way valve.

[0015] The output end of the high-pressure gas storage module is connected with the reaction chamber via the third pressure regulating valve and the third two-way valve.

[0016] As an embodiment, the high-pressure gas storage module comprises an air pump, a one-way valve and a gas storage tank.

[0017] The output end of the air pump is connected with the input end of the one-way valve, the output end of the one-way valve is connected with the input end of the gas storage tank, and the output end of the gas storage tank is the output end of the high-pressure gas storage module.

[0018] As an embodiment, the high-pressure gas storage module further comprises a water-gas separator, and the output end of the air pump is connected with the input end of the one-way valve via the water-gas separator.

[0019] As an embodiment, the high-pressure gas storage module further comprises a three-way valve, which comprises a first connection end, a second connection end and a third connection end.

[0020] The first connection end of the three-way valve is connected with the output end of the air pump, the second connection end of the three-way valve is connected with the input end of the one-way valve, and the third connection end of the three-way valve is connected with air.

[0021] As an embodiment, a trace liquid adding module is further included, and the output end of the trace liquid adding module is connected with a plurality of synthesis columns of the reaction chamber.

[0022] The trace liquid adding module is used for adding trace liquid to the plurality of synthesis columns.

[0023] As an embodiment, the trace liquid adding module comprises a solution carrier, an inlet valve, a distribution pump, an outlet valve and a distribution block.

[0024] The solution carrier is filled with a target solution.

[0025] The liquid inlet valve is provided with a first inlet end and a first outlet end, the first inlet end of the liquid inlet valve is communicated with the solution carrier, and the first outlet end of the liquid inlet valve is communicated with the liquid distribution pump;

[0026] The liquid outlet valve is provided with a second inlet end and a second outlet end, the second inlet end of the liquid outlet valve is communicated with the liquid distribution pump, and the second outlet end of the liquid outlet valve is communicated with the liquid distribution block;

[0027] The liquid distribution block comprises a plurality of liquid distribution ports, and each liquid distribution port is provided with an electromagnetic valve;

[0028] The liquid distribution pump is used for driving the target solution contained in the solution carrier to be transmitted to the liquid distribution block through the liquid inlet valve, the liquid distribution pump and the liquid outlet valve, so that the target solution is distributed and output to the plurality of synthesis columns through the plurality of electromagnetic valves of the liquid distribution block.

[0029] As an embodiment, each electromagnetic valve of each liquid distribution port is connected with an infusion needle with the same inner diameter.

[0030] Compared with the prior art, the reaction device has the following beneficial effects:

[0031] The reaction device can store high-pressure gas through the high-pressure gas storage module, then output the first gas pressure to the cylinder through the first pressure regulating module, so that the cylinder tightly presses the reaction bin; then output the second gas pressure to the reaction bin through the second pressure regulating module, so that the reagents in the synthesis columns fully react under the action of the second gas pressure; since the gas pressure value of the second gas pressure is less than the first gas pressure, the reagents in the synthesis columns can fully contact and react under the action of the second gas pressure in the case that the cylinder tightly presses the reaction bin. Moreover, the third gas pressure is output to the reaction bin through the third pressure regulating module, since the gas pressure value of the third gas pressure is greater than the second gas pressure and less than the first gas pressure, the cylinder can still tightly press the reaction bin, and the gas pressure value of the third gas pressure is less than the first gas pressure, so that the action force of the third gas pressure on the waste liquid after reaction is greater than that of the second gas pressure, the waste liquid can be discharged into the waste liquid bottle through the greater action force, and waste liquid residue is prevented.

[0032] In order to better understand and implement, the application will be described in detail below with reference to the drawings. BRIEF DESCRIPTION OF DRAWINGS

[0033] Figure 1 The schematic diagram of the reaction device of one embodiment of the application;

[0034] Figure 2 The schematic diagram of the micro-liquid adding module of the reaction device of one embodiment of the application.

[0035] Figure 3The schematic diagram of the liquid distribution block of the trace liquid adding module of the reaction device of one embodiment of the utility model.

[0036] Figure 4 The connection schematic diagram of the upper computer of the trace liquid adding module of the reaction device of one embodiment of the utility model.

[0037] Figure 5 The motion curve schematic diagram of the liquid distribution pump of the trace liquid adding module of the reaction device of one embodiment of the utility model.

[0038] 1, solution carrier;2, liquid inlet valve;3, liquid distribution pump;4, liquid outlet valve;5, liquid distribution block;6, electromagnetic valve;7, steel needle;8, upper computer;9, water gas separator;10, three-way valve;11, check valve;12, gas storage tank;13, first pressure regulating valve;14, second pressure regulating valve;15, third pressure regulating valve;16, first two-way valve;17, second two-way valve;18, third two-way valve;19, reaction bin;20, waste liquid bottle. DETAILED DESCRIPTION

[0039] To further illustrate the embodiments, the present application provides drawings. These drawings are part of the disclosure of the present application, which mainly serve to illustrate the embodiments, and can be explained in conjunction with the related description of the specification to explain the operating principle of the embodiments. With reference to these contents, those skilled in the art should understand other possible implementation manners and advantages of the present application.

[0040] In the description of the present application, it should be understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "left", "right", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential" and the like are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0041] Please refer to Figure 1 The embodiment provides a reaction device, which comprises a high-pressure gas storage module, a first pressure regulating module, a second pressure regulating module, a third pressure regulating module, a gas cylinder, a reaction bin 19 and a waste liquid bottle 20;The reaction bin 19 comprises a plurality of synthesis columns;

[0042] The output end of the high-pressure gas storage module is connected with the first input end of the first pressure regulating module, the second input end of the second pressure regulating module and the third input end of the third pressure regulating module respectively;

[0043] The first output end of the first pressure regulating module is connected with the cylinder, and the high-pressure gas storage module outputs a first gas pressure to the cylinder through the first pressure regulating module, so that the cylinder presses the reaction bin 19;

[0044] The second output end of the second pressure regulating module is connected with the reaction bin 19, and the high-pressure gas storage module outputs a second gas pressure to the reaction bin 19 through the second pressure regulating module, so that the reagents in the plurality of synthesis columns fully react under the action of the second gas pressure; the gas pressure value of the second gas pressure is less than the first gas pressure;

[0045] The third output end of the third pressure regulating module is connected with the reaction bin 19, and the high-pressure gas storage module outputs a third gas pressure to the reaction bin 19 through the third pressure regulating module, so that the reacted waste liquid in the plurality of synthesis columns flows into the waste liquid bottle 20 under the action of the third gas pressure; wherein the gas pressure value of the third gas pressure is greater than the second gas pressure, and the gas pressure value of the third gas pressure is less than the first gas pressure.

[0046] As a feasible embodiment, the first pressure regulating module comprises a first pressure regulating valve 13 and a first two-way valve 16.

[0047] The output end of the high-pressure gas storage module is connected with the cylinder in sequence through the first pressure regulating valve 13 and the first two-way valve 16.

[0048] As a feasible embodiment, the second pressure regulating module comprises a second pressure regulating valve 14 and a second two-way valve 17.

[0049] The output end of the high-pressure gas storage module is connected with the reaction bin 19 in sequence through the second pressure regulating valve 14 and the second two-way valve 17.

[0050] As a feasible embodiment, the third pressure regulating module comprises a third pressure regulating valve 15 and a third two-way valve 18.

[0051] The output end of the high-pressure gas storage module is connected with the reaction bin 19 in sequence through the third pressure regulating valve 15 and the third two-way valve 18.

[0052] As a feasible embodiment, the high-pressure gas storage module comprises an air pressure pump, a one-way valve 11 and a gas storage tank 12.

[0053] The output end of the air pressure pump is connected with the input end of the one-way valve 11, the output end of the one-way valve 11 is connected with the input end of the gas storage tank 12, and the output end of the gas storage tank 12 is the output end of the high-pressure gas storage module.

[0054] As a feasible embodiment, the high-pressure gas storage module further comprises a water-gas separator 9; the output end of the air compressor pump is connected with the input end of the one-way valve 11 via the water-gas separator 9.

[0055] As a feasible embodiment, the high-pressure gas storage module further comprises a three-way valve 10, which comprises a first connection end, a second connection end and a third connection end;

[0056] The first connection end of the three-way valve 10 is connected with the output end of the air compressor pump, the second connection end of the three-way valve 10 is connected with the input end of the one-way valve 11, and the third connection end of the three-way valve 10 is connected with air.

[0057] The present application can store high-pressure gas through the high-pressure gas storage module, then output the first air pressure to the air cylinder through the first pressure regulating module, so that the air cylinder presses the reaction bin 19; then output the second air pressure to the reaction bin 19 through the second pressure regulating module, so that the reagents in the plurality of synthesis columns fully react under the action of the second air pressure; since the air pressure value of the second air pressure is less than the first air pressure, the reagents in the synthesis columns can be fully contacted and reacted through the action force of the second air pressure on the reagents under the condition that the air cylinder tightly presses the reaction bin 19. Moreover, the third air pressure is output to the reaction bin 19 through the third pressure regulating module, since the air pressure value of the third air pressure is greater than the second air pressure and the air pressure value of the third air pressure is less than the first air pressure, the air cylinder can still tightly press the reaction bin 19, and the air pressure value of the third air pressure is less than the first air pressure, so that the action force of the third air pressure on the waste liquid after reaction is greater than that of the second air pressure, which can discharge the waste liquid into the waste liquid bottle 20 through a greater action force, preventing the waste liquid from being left.

[0058] Please refer to Figures 2-5 As a feasible embodiment, the micro-liquid adding module further comprises a solution carrier, a liquid inlet valve, a liquid distribution pump, a liquid outlet valve and a liquid distribution block.

[0059] The micro-liquid adding module is used for adding micro-liquid to the plurality of synthesis columns.

[0060] As a feasible embodiment, the micro-liquid adding module comprises a solution carrier, a liquid inlet valve, a liquid distribution pump, a liquid outlet valve and a liquid distribution block.

[0061] The solution carrier is provided with a target solution.

[0062] The liquid inlet valve is provided with a first inlet end and a first outlet end, the first inlet end of the liquid inlet valve is in communication with the solution carrier, and the first outlet end of the liquid inlet valve is in communication with the liquid distribution pump.

[0063] The liquid outlet valve is provided with a second inlet end and a second outlet end, the second inlet end of the liquid outlet valve is communicated with the liquid distribution pump, and the second outlet end of the liquid outlet valve is communicated with the liquid distribution block;

[0064] The liquid distribution block comprises a plurality of liquid distribution ports, and each liquid distribution port is provided with an electromagnetic valve.

[0065] The liquid distribution pump is used to drive the reagent solution contained in the solution carrier to be transmitted to the liquid distribution block through the liquid inlet valve, the liquid distribution pump and the liquid outlet valve, so that the reagent solution is distributed and output to the plurality of synthesis columns through the plurality of electromagnetic valves of the liquid distribution block.

[0066] As a feasible embodiment, each electromagnetic valve of each liquid distribution port is respectively connected with a transfusion needle with the same inner diameter.

[0067] In a feasible embodiment, the transfusion needle connected with the electromagnetic valve 6 is a steel needle 7 made of stainless steel.

[0068] In a feasible embodiment, the liquid inlet valve 2 is a liquid inlet electromagnetic valve, and the liquid outlet valve 4 is a liquid outlet electromagnetic valve.

[0069] In a feasible embodiment, the liquid distribution pump 3 is a plunger pump, the first outlet end of the liquid inlet valve 2 is communicated with the plunger pump, and the second inlet end of the liquid outlet valve 4 is communicated with the plunger pump.

[0070] In a feasible embodiment, the liquid distribution pump 3 is a peristaltic pump, the first outlet end of the liquid inlet valve 2 is communicated with the peristaltic pump, and the second inlet end of the liquid outlet valve 4 is communicated with the peristaltic pump.

[0071] In a feasible embodiment, the liquid distribution pump 3 is a constant pump, the first outlet end of the liquid inlet valve 2 is communicated with the constant pump, and the second inlet end of the liquid outlet valve 4 is communicated with the constant pump.

[0072] In a feasible embodiment, the liquid distribution pump 3 is a syringe pump, the first outlet end of the liquid inlet valve 2 is communicated with the syringe pump, and the second inlet end of the liquid outlet valve 4 is communicated with the syringe pump.

[0073] In a feasible embodiment, the first inlet end of the liquid inlet valve 2 is communicated with the bottom of the solution carrier 1 through a transfusion pipe.

[0074] As shown in Figure 4 In a feasible embodiment, an upper computer 8 is further included, and the upper computer 8 is connected with the liquid inlet valve 2, the liquid distribution pump 3, the liquid outlet valve 4 and each electromagnetic valve 6.

[0075] The host computer 8 drives the inlet valve 2 to open and the outlet valve 4 to close, so that the dispensing pump 3 draws the reagent solution of the solution carrier 1 through the inlet valve 2;

[0076] The host computer 8 drives the inlet valve 2 to close, the outlet valve 4 to open, and each of the solenoid valves 6 to open, so that the dispensing pump 3 outputs the reagent solution to each of the solenoid valves 6 via the outlet valve 4, so that the reagent solution is dispensed and output through the multiple solenoid valves 6 of the dispensing block 5.

[0077] The host computer 8 drives the solenoid valves 6 to open sequentially according to a preset order and time difference, so that each solenoid valve 6 outputs the same amount of target liquid.

[0078] Among them, such as Figures 2-5 As shown, inlet valve 2 is opened, and dispensing pump 3 draws the reagent solution from the solution carrier. After the solution is drawn, inlet valve 2 is closed, and outlet valve 4 and eight solenoid valves 6 are opened. Simultaneously, dispensing pump 3 injects liquid into dispensing block 5 through outlet valve 4, and dispensing and batch sampling are performed by infusion needles 7 connected through eight solenoid valves 6. At this time, the liquid volume output by each infusion needle 7 is weighed by a balance and sorted in ascending order to obtain a preset order. Then, the compensation amount (δV1, δV2...δV8; δV8 is 0) of each of the other infusion needles 7 is calculated based on the maximum liquid volume. The motion curve of dispensing pump 3 is shown in the figure. Figure 5 As shown, the displacement s during the acceleration phase is... 1 / 2at 2 The compensation time (t1, t2, ..., t8) of each solenoid valve 6 can be obtained according to the displacement-volume conversion formula of the dispensing pump 3 as a preset time difference. Then, the host computer drives the solenoid valves to open sequentially according to the preset order and time difference. The compensation time refers to the delayed closing time of the corresponding solenoid valve 6 relative to the solenoid valve 6 with the maximum liquid volume. A balance is used to weigh the weight change of the object receiving infusion from each infusion needle 7 to determine the liquid volume output by each infusion needle 7. The object receiving infusion from each infusion needle 7 can be an identical container.

[0079] The micro-liquid addition module of the reaction device of this utility model can add reagent solution in solution carrier 1 through multiple dispensing ports of dispensing block 5, so that multiple targets can simultaneously obtain reagent solution through the corresponding dispensing ports, improving the consistency of liquid dispensing. Moreover, each dispensing port is equipped with a solenoid valve 6, which can be controlled to better regulate the liquid output of the corresponding dispensing port. The solenoid valve 6 is controlled by the host computer 8, so the host computer 8 can drive the solenoid valve 6 to open sequentially according to the preset order and time difference, so that each solenoid valve 6 outputs the same amount of target liquid, which can more effectively improve the consistency of liquid dispensing.

[0080] The above-described embodiments are merely illustrative of several embodiments of the present application, which are described in more detail and in a specific manner, but should not be understood as a limitation on the scope of protection of the present application. It should be noted that, for those skilled in the art, several modifications and improvements can be made without departing from the concept of the present application, and these all belong to the scope of protection of the present application.

Claims

1. A reaction apparatus, characterized in that, include: The system comprises a high-pressure gas storage module, a first pressure regulating module, a second pressure regulating module, a third pressure regulating module, a cylinder, a reaction chamber, and a waste liquid bottle; the reaction chamber includes multiple synthesis columns. The output terminal of the high-pressure gas storage module is connected to the first input terminal of the first pressure regulating module, the second input terminal of the second pressure regulating module, and the third input terminal of the third pressure regulating module, respectively. The first output terminal of the first pressure regulating module is connected to the cylinder, and the high-pressure gas storage module outputs a first gas pressure to the cylinder via the first pressure regulating module, so that the cylinder presses the reaction chamber tightly. The second output terminal of the second pressure regulating module is connected to the reaction chamber. The high-pressure gas storage module outputs a second gas pressure to the reaction chamber via the second pressure regulating module, so that the reagents in the plurality of synthesis columns react fully under the action of the second gas pressure. The gas pressure value of the second gas pressure is less than the first gas pressure. The third output terminal of the third pressure regulating module is connected to the reaction chamber. The high-pressure gas storage module outputs a third gas pressure to the reaction chamber via the third pressure regulating module, so that the waste liquid after the reaction in the multiple synthesis columns flows into the waste liquid bottle under the action of the third gas pressure. The gas pressure value of the third gas pressure is greater than that of the second gas pressure and less than that of the first gas pressure.

2. The reaction apparatus according to claim 1, characterized in that: The first pressure regulating module includes a first pressure regulating valve and a first two-way valve; The output end of the high-pressure gas storage module is connected to the cylinder in sequence via the first pressure regulating valve and the first two-way valve.

3. The reaction apparatus according to claim 1, characterized in that: The second pressure regulating module includes a second pressure regulating valve and a second two-way valve; The output end of the high-pressure gas storage module is connected to the reaction chamber via the second pressure regulating valve and the second two-way valve in sequence.

4. The reaction apparatus according to claim 1, characterized in that: The third pressure regulating module includes a third pressure regulating valve and a third two-way valve; The output end of the high-pressure gas storage module is connected to the reaction chamber via the third pressure regulating valve and the third two-way valve in sequence.

5. The reaction apparatus according to claim 1, characterized in that: The high-pressure gas storage module includes an air compressor, a one-way valve, and a gas storage tank. The output end of the air compressor is connected to the input end of the one-way valve, the output end of the one-way valve is connected to the input end of the air storage tank, and the output end of the air storage tank is the output end of the high-pressure air storage module.

6. The reaction apparatus according to claim 5, characterized in that: The high-pressure gas storage module also includes a water-gas separator; the output end of the air compressor is connected to the input end of the one-way valve via the water-gas separator.

7. The reaction apparatus according to claim 5, characterized in that: The high-pressure gas storage module also includes a three-way valve, which includes a first connection end, a second connection end, and a third connection end. The first connection end of the three-way valve is connected to the output end of the air compressor, the second connection end of the three-way valve is connected to the input end of the one-way valve, and the third connection end of the three-way valve is connected to external air.

8. The reaction apparatus according to claim 1, characterized in that: It also includes a trace liquid addition module, the output of which is connected to multiple synthesis columns in the reaction chamber; The trace liquid addition module is used to add trace amounts of liquid to the plurality of synthesis columns.

9. The reaction apparatus according to claim 8, characterized in that: The micro-liquid addition module includes a solution carrier, an inlet valve, a dispensing pump, an outlet valve, and a dispensing block; The solution carrier contains the target solution; The liquid inlet valve has a first inlet end and a first outlet end. The first inlet end of the liquid inlet valve is connected to the solution carrier, and the first outlet end of the liquid inlet valve is connected to the liquid separator pump. The liquid outlet valve is provided with a second inlet end and a second outlet end. The second inlet end of the liquid outlet valve is connected to the liquid separator pump, and the second outlet end of the liquid outlet valve is connected to the liquid separator block. The liquid distribution block includes multiple liquid distribution ports, and each liquid distribution port is equipped with a solenoid valve. The dispensing pump is used to drive the target solution contained in the solution carrier to be transferred to the dispensing block via the inlet valve, the dispensing pump and the outlet valve, so that the target solution can be dispensed and output to the multiple synthesis columns through multiple solenoid valves of the dispensing block.

10. The reaction apparatus according to claim 9, characterized in that: Each of the solenoid valves at each of the aforementioned dispensing ports is connected to an infusion needle with the same inner diameter.

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