Protein incubation box capable of recycling antibody

Abstract

The utility model discloses a protein incubation box capable of recovering antibodies, and relates to the technical field of molecular biology experiments, the protein incubation box comprises a placing frame, a box body and a plurality of antibody boxes, the box body is slidably connected to the inner bottom wall of the placing frame, the antibody boxes are arranged in the box body, the bottoms of the antibody boxes are provided with recovery pipes, and the recovery pipes are connected with the box body. An electromagnetic valve is arranged on the recovery pipe, a recovery drawer is arranged below the antibody box, a microfiltration membrane is movably arranged at the top of the recovery drawer, and fixing assemblies for fixing the microfiltration membrane are arranged on the two sides of the top of the recovery drawer. According to the utility model, an antibody is placed in the antibody box, and the non-free antibody in the antibody box is enabled to fall into the recovery drawer through the recovery pipe by opening the electromagnetic valve corresponding to the antibody box, and then discharged waste liquid is treated in a centralized manner through the microfiltration membrane, and unconsumed reagents are recovered; and the microporous filter membrane which can be quickly detached is convenient to replace.

Description

Technical Field

[0001] This invention relates to the field of molecular biology experimental technology, specifically a protein incubation box for recyclable antibodies. Background Technology

[0002] A protein incubation box is a device or container specifically designed for incubating protein samples in biological experiments. It is mainly used for experimental steps such as antibody incubation, enzyme reactions, and immunoblotting.

[0003] For example, a protein antibody incubation box described in patent publication number CN219417478 U has the following scheme: by rotating a bidirectional screw, two threaded blocks move in opposite directions. When the threaded blocks move, they cause the connecting rod to deflect at an angle. The connecting rod causes the horizontal plate to move within the box, thereby changing the longitudinal distance between the horizontal plate and the box. Through the elastic tension of the spring, the pressure roller abuts against both sides of the longitudinal telescopic plate, thereby fixing the longitudinal telescopic plate and dividing the box into multiple independent spaces. This avoids cross-contamination, facilitates the adjustment of the incubation space, and helps improve the incubation efficiency of protein antibodies.

[0004] After antibody incubation, the antibody cassette may contain unreacted solution and antibody. Usually, the waste liquid is discarded, and the residual antibody is easily discarded along with the waste liquid, resulting in a certain waste of resources. It is also inconvenient to recycle unused antibodies. At the same time, during the antibody reaction, the operator needs to manually shake the antibody. Manual shaking is not only cumbersome, but it is also difficult to keep the force and frequency consistent, which can easily lead to poor reproducibility of experimental results. Utility Model Content

[0005] The purpose of this invention is to provide a protein incubation box for recyclable antibodies to solve the problems in the prior art.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] A protein incubation cassette for recyclable antibodies, comprising:

[0008] Placement box;

[0009] The box body is slidably connected to the inner bottom wall of the placement frame;

[0010] It also includes several antibody boxes, which are disposed inside the box body. A recovery tube is installed at the bottom of the antibody box, and a solenoid valve is provided on the recovery tube. A recovery drawer is provided below the antibody box, and a microporous filter membrane is movably disposed on the top of the recovery drawer. Fixing components for fixing the microporous filter membrane are provided on both sides of the top of the recovery drawer.

[0011] Based on the above technical solutions, this utility model also provides the following optional technical solutions:

[0012] In one alternative embodiment: the fixing component includes placement slots, which are formed on both sides of the top of the recycling drawer. A retaining plate is movably connected inside the placement slot, and the retaining plate is installed on both sides of the top of the microporous filter membrane. Movable slots are also formed on both sides of the top of the recycling drawer. A spring is installed on one side of the inner wall of the movable slot, and a buckle plate is installed at one end of the spring. A retaining post is installed on one side of the buckle plate. The retaining plate has slots on both sides that match the positions of the retaining posts. The retaining posts are movably connected inside the slots. Movable slots are formed on both sides inside the recycling drawer. A limiting plate is slidably connected inside the movable slot. A sliding post is installed at one end of the limiting plate, and one end of the sliding post passes through the spring.

[0013] In one alternative: the placement frame is provided with a shaking component for shaking the box.

[0014] In one alternative embodiment: the shaking assembly includes a motor mounted on the inner wall of one side of the placement frame; an eccentric turntable is mounted on the output end of the motor; a protruding post is provided on the eccentric turntable; a rack is mounted on the bottom end of the box near the motor; a sector gear meshes with each other above the rack; a strip frame is mounted on the upper end of the sector gear; a rotating column is rotatably connected to the connection between the strip frame and the sector gear; the rotating column is fixedly connected to the placement frame; a through groove is provided on the strip frame; and the protruding post is slidably connected inside the through groove.

[0015] In one alternative: a fixing block is installed around the bottom of the box, and a guide rod is slidably connected inside the fixing block, the guide rod being installed on both sides of the inner wall of the placement frame.

[0016] In one alternative: the recycling drawer has a handle on one side.

[0017] In one alternative: the top of the box is provided with a lid.

[0018] In one alternative: the card is L-shaped.

[0019] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0020] 1. This utility model, by placing antibodies inside an antibody box and installing a detachable microporous filter membrane, can efficiently separate unconsumed antibody solution from waste liquid. At this time, the unfree antibodies in the antibody box are released by opening the corresponding solenoid valve of the antibody box, allowing the solution to fall into the recovery drawer through the recovery tube. Then, the discharged waste liquid is centrally processed and the unconsumed reagents are recovered through the microporous filter membrane. The microporous filter membrane is designed for quick removal and easy replacement.

[0021] 2. This utility model starts the motor, which drives the eccentric turntable and the convex column to run, thereby driving the sector gear and rack to run, and then causing the box to shake. The mechanical vibration accelerates the combination reaction of the solution and shortens the experimental time. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the structure of this utility model.

[0023] Figure 2 This is a partial structural schematic diagram of the present invention.

[0024] Figure 3 This is a schematic diagram of a partial rear view of the present invention.

[0025] Figure 4 This is a partial structural diagram of the shaking component of this utility model.

[0026] Figure 5 This is a partial structural diagram of the fixing component of this utility model.

[0027] Figure 6 This utility model Figure 5 A magnified structural diagram of A in the diagram.

[0028] Figure 7 This is a top sectional view of the fixing component in this utility model.

[0029] The components are as follows: 100, placement frame; 200, box body; 301, antibody box; 302, recovery tube; 303, recovery drawer; 304, microporous filter membrane; 305, solenoid valve; 401, placement slot; 402, clamping plate; 403, moving slot; 404, spring; 405, buckle plate; 406, clamping post; 407, clamping groove; 408, sliding post; 409, limiting plate; 410, movable slot; 501, motor; 502, eccentric turntable; 503, rack; 504, sector gear; 505, strip frame; 506, rotating post; 507, through slot; 508, protruding post; 601, fixing block; 602, guide rod. Detailed Implementation

[0030] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments.

[0031] In one embodiment, such as Figures 1-7As shown, a protein incubation box for recoverable antibodies includes: a placement frame 100, a box body 200, and a plurality of antibody boxes 301. The box body 200 is slidably connected to the inner bottom wall of the placement frame 100. The antibody boxes 301 are disposed inside the box body 200. A recovery tube 302 is installed at the bottom of the antibody box 301. A solenoid valve 305 is provided on the recovery tube 302. A recovery drawer 303 is provided below the antibody box 301. A microporous filter membrane 304 is movably disposed on the top of the recovery drawer 303. Fixing components for fixing the microporous filter membrane 304 are provided on both sides of the top of the recovery drawer 303. Unused antibodies inside the antibody box 301 are recovered into the recovery drawer 303 by opening the solenoid valve 305 on the corresponding antibody box 301, and then the antibodies are recovered through the recovery tube 302 into the recovery drawer 303. After being filtered by the microporous filter membrane 304, the antibodies can be easily recovered.

[0032] In one embodiment, such as Figure 5 , Figure 6 and Figure 7 As shown, the fixing assembly includes a placement slot 401, which is formed on both sides of the top of the recycling drawer 303. A retaining plate 402 is movably connected inside the placement slot 401. The retaining plate 402 is installed on both sides of the top of the microporous filter membrane 304. Moving slots 403 are also formed on both sides of the top of the recycling drawer 303. A spring 404 is installed on one side of the inner wall of the moving slot 403. A buckle plate 405 is installed at one end of the spring 404. A retaining post 406 is installed on one side of the buckle plate 405. The retaining plate 402 has retaining grooves 407 on both sides that match the positions of the retaining posts 406. The retaining posts 406 are movably connected inside the retaining grooves 407. Moving slots 410 are formed on both sides inside the recycling drawer 303. The internal sliding connection is a limiting plate 409, one end of which is equipped with a sliding post 408, and one end of the sliding post 408 is inserted through a spring 404. When it is necessary to fix the microporous filter membrane 304, the buckle plates 405 on both sides are pushed to both sides and the spring 404 is squeezed simultaneously. At the same time, the spring 404 drives the sliding post 408 to move, and the sliding post 408 drives the limiting plate 409 to slide inside the movable groove 410, so that the locking post 406 approaches the locking groove 407. Then the locking plate 402 is placed inside the placement groove 401, and then the buckle plate 405 is released. Under the action of the spring 404, the buckle plate 405 drives the locking post 406 to reset. At this time, the locking post 406 is locked into the inside of the locking groove 407, fixing the locking plate 402 on the recycling drawer 303.

[0033] In one embodiment, such as Figure 2 , Figure 3 and Figure 4As shown, the placement frame 100 is equipped with a shaking assembly for shaking the box 200. The shaking assembly includes a motor 501, which is mounted on the inner wall of one side of the placement frame 100. An eccentric turntable 502 is mounted on the output end of the motor 501. The eccentric turntable 502 has a protrusion 508. A rack 503 is mounted on the bottom end of the box 200 near the motor 501. A sector gear 504 meshes with each other above the rack 503. A strip frame 505 is mounted on the upper end of the sector gear 504. A rotating column 506 is rotatably connected to the connection between the strip frame 505 and the sector gear 504. The rotating column 506 is fixed. Connected to the placement frame 100, the strip frame 505 has a through groove 507, and the protruding post 508 is slidably connected inside the through groove 507. By starting the motor 501, the eccentric turntable 502 is driven to rotate, thereby driving the protruding post 508 to run synchronously, so that the protruding post 508 slides vertically in the through groove 507. When the eccentric turntable 502 drives the protruding post 508 to the top and bottom positions of the through groove 507, the movement of the protruding post 508 causes the through groove 507 and the strip frame 505 to tilt, thereby driving the sector gear 504 to run. At this time, the sector gear 504 and the rack 503 mesh with each other, causing the rack 503 to move, and then the rack 503 drives the box body 200 to shake.

[0034] In one embodiment, such as Figure 1 , Figure 2 and Figure 3 As shown, a fixing block 601 is installed around the bottom of the box 200. A guide rod 602 is slidably connected inside the fixing block 601. The guide rod 602 is installed on both sides of the inner wall of the placement frame 100. This allows the fixing block 601 to move on the guide rod 602 when the rack 503 moves, thereby guiding the movement of the box 200 and preventing the box 200 and the rack 503 from disengaging from the sector gear 504.

[0035] In one embodiment, such as Figure 1 As shown, a handle is provided on one side of the recycling drawer 303; the handle makes it easy to remove the recycling drawer 303.

[0036] In one embodiment, such as Figure 1 As shown, the top of the box 200 is provided with a box cover; this facilitates the placement of antibodies inside the antibody box 301.

[0037] In one embodiment, such as Figure 5 and Figure 6 As shown, the card plate 402 is L-shaped; it is convenient to put the card plate 402 into the card slot 407, and it is also convenient to lift the microporous filter membrane 304.

[0038] The above embodiment discloses a protein incubation box for recyclable antibodies. First, the antibody is placed inside the antibody box 301. Then, a microporous filter membrane 304 is installed on the recycling drawer 303. By pushing the two side plates 405 to the sides and simultaneously squeezing the spring 404, the spring 404 drives the sliding column 408 to move. The sliding column 408 drives the limiting plate 409 to slide inside the movable groove 410, thereby bringing the locking post 406 closer to the locking slot 407. Then, the locking plate 402 is placed inside the placement groove 401. After releasing the plate 405, under the action of the spring 404, the plate 405 drives the locking post 406 to reset. At this time, the locking post 406 is locked into the inside of the locking slot 407, fixing the locking plate 402 to the recycling drawer 303. Finally, the lid of the box body 200 is closed. At this point, the reaction rate can be accelerated by shaking the box 200. By starting the motor 501, the eccentric turntable 502 is rotated, which in turn drives the protruding post 508 to run synchronously. This allows the protruding post 508 to slide vertically in the through groove 507. When the eccentric turntable 502 drives the protruding post 508 to the top and bottom of the through groove 507, the movement of the protruding post 508 causes the through groove 507 and the strip frame 505 to tilt, which in turn drives the sector gear 504 to run. At this time, the sector gear 504 and the rack 503 mesh with each other, causing the rack 503 to move. Then the rack 503 drives the box 200 to shake. At this time, the fixing block 601 moves on the guide rod 602 to guide the shaking of the box 200 and prevent the box 200 and the rack 503 from disengaging from the sector gear 504.

[0039] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A protein incubation cassette for recyclable antibodies, comprising: Placement box (100); Box body (200), said box body (200) is slidably connected to the inner bottom wall of the placement frame (100); The invention is characterized by further comprising a plurality of antibody boxes (301), wherein the antibody boxes (301) are disposed inside the box body (200), a recovery tube (302) is installed at the bottom of the antibody box (301), a solenoid valve (305) is provided on the recovery tube (302), a recovery drawer (303) is provided below the antibody box (301), a microporous filter membrane (304) is movably disposed at the top of the recovery drawer (303), and fixing components for fixing the microporous filter membrane (304) are provided on both sides of the top of the recovery drawer (303).

2. The protein incubation box for recyclable antibodies according to claim 1, characterized in that, The fixing assembly includes a placement slot (401) located on both sides of the top of the recycling drawer (303). A retaining plate (402) is movably connected inside the placement slot (401). The retaining plate (402) is installed on both sides of the top of the microporous filter membrane (304). Moving slots (403) are also provided on both sides of the top of the recycling drawer (303). A spring (404) is installed on one side of the inner wall of the moving slot (403). A buckle plate (405) is installed at one end of the spring (404). A locking post (406) is installed on one side of the card plate (402). The card plate (402) has card slots (407) on both sides that match the position of the locking post (406). The locking post (406) is movably connected to the inside of the card slot (407). Movable slots (410) are opened on both sides inside the recycling drawer (303). A limiting plate (409) is slidably connected inside the movable slot (410). A sliding post (408) is installed at one end of the limiting plate (409). A spring (404) passes through one end of the sliding post (408).

3. The protein incubation cassette for recyclable antibodies according to claim 1, characterized in that, The placement frame (100) is provided with a shaking component for shaking the box (200).

4. The protein incubation cassette for recyclable antibodies according to claim 3, characterized in that, The shaking assembly includes a motor (501), which is mounted on the inner wall of one side of the placement frame (100). An eccentric turntable (502) is mounted on the output end of the motor (501). A protruding post (508) is provided on the eccentric turntable (502). A rack (503) is mounted on the bottom end of the box (200) near the motor (501). A sector gear (504) meshes with each other above the rack (503). A strip frame (505) is mounted on the upper end of the sector gear (504). A rotating column (506) is rotatably connected at the connection between the strip frame (505) and the sector gear (504). The rotating column (506) is fixedly connected to the placement frame (100). A through groove (507) is opened on the strip frame (505). The protruding post (508) is slidably connected inside the through groove (507).

5. The protein incubation cassette for recyclable antibodies according to claim 1, characterized in that, Fixing blocks (601) are installed around the bottom of the box (200). A guide rod (602) is slidably connected inside the fixing block (601). The guide rod (602) is installed on both sides of the inner wall of the placement frame (100).

6. The protein incubation cassette for recyclable antibodies according to claim 1, characterized in that, The recycling drawer (303) has a handle on one side.

7. The protein incubation cassette for recyclable antibodies according to claim 1, characterized in that, The top of the box (200) is provided with a lid.

8. A protein incubation cassette for recyclable antibodies according to claim 2, characterized in that, The card plate (402) is L-shaped.

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