Microscope multiband adjustable fluorescent device

Abstract

The utility model discloses a multiband adjustable fluorescence device of a microscope, which is arranged on a microscope main body and comprises a fluorescence assembly and a mercury lamp box, the fluorescence assembly comprises a shell, a turntable adjusting unit and a shading unit, the mercury lamp box is connected with the shell, the turntable adjusting unit comprises a turntable shell, a turntable and a plurality of fluorescence color filtering units, the fluorescent color filtering units are annularly distributed on the turntable, and different fluorescent color filtering units are cut into a light path when the turntable rotates; the shading unit comprises a shading piece, a first shifting rod, a second shifting rod, a handle, a screw column and an adjusting screw, when the handle is shifted to drive the second shifting rod to rotate, the stand column boss abuts against and pushes the first shifting rod to rotate, and therefore the shading piece is cut out of or cut into the light path. The device can quickly realize replacement and observation of fluorescent color filtering units with different wave bands, is convenient for cutting off the whole light path to prevent rapid attenuation of a fluorescent specimen caused by long-time irradiation, improves the utilization rate of the light path, and is convenient for rapid data collection.

Description

Technical Field

[0001] This utility model belongs to the field of microscope technology, specifically relating to a multi-band adjustable fluorescence device for microscopes. Background Technology

[0002] In traditional microscopic observation modes and the series of supporting techniques, what is observed is a colored structure against a white background. However, in the in-situ detection of molecules, the colored products of the reaction are sometimes interfered with by the background, making it difficult to judge the color development results and the distribution of molecules. Fluorescence microscopy solves this problem very well.

[0003] Fluorescence microscopy uses ultraviolet light as a light source to illuminate the reactants of fluorescent stains or fluorescently labeled genes, causing the structure under test to emit specific fluorescence. This results in a completely black background, with the target structure emitting light and reducing the influence of impurities. The fluorescence apparatus guides light of the corresponding wavelength from an external light source to illuminate the fluorescent dye and collects the emitted fluorescence. However, a single specimen often requires multiple fluorescent dyes, necessitating the use of different wavelengths. This necessitates replacing different fluorescent filter units as needed, making the operation complex, time-consuming, and labor-intensive. Furthermore, it is difficult to avoid fluorescence attenuation and quenching on the object under test, reducing light utilization and hindering the observation of imaging effects excited by different wavelengths within a short time. Utility Model Content

[0004] The purpose of this invention is to address the above-mentioned problems by proposing a multi-band adjustable fluorescence device for microscopes. This device allows for easy rotation and replacement of different band fluorescence filter units to cut into the optical path, preventing rapid attenuation of fluorescent specimens due to prolonged irradiation, improving light utilization, and simplifying operation and maintenance.

[0005] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0006] This utility model proposes a multi-band adjustable fluorescence device for a microscope, which is installed on the microscope body and includes a fluorescence component and a mercury lamp housing. The fluorescence component includes a housing, a rotary adjustment unit, and a light-shielding unit. The mercury lamp housing is connected to the housing, wherein:

[0007] The housing has a first light-transmitting hole on the upper wall and a second light-transmitting hole on the lower wall, and the first light-transmitting hole and the second light-transmitting hole are coaxial;

[0008] The turntable adjustment unit includes a turntable shell, a turntable, and several fluorescent filter units. The turntable shell is detachably connected to the housing to form a cavity. The turntable and the fluorescent filter units are both built into the cavity. The fluorescent filter units are arranged in a ring on the turntable. When the turntable rotates, different fluorescent filter units are cut into the optical path.

[0009] The light-shielding unit, built into the cavity, includes a light-shielding plate, a first lever, a second lever, a handle, a screw post, and an adjusting screw. The light-shielding plate is connected to the first lever, which is rotatably connected to the housing via the screw post. The second lever is connected to the handle and has a vertically positioned boss at the end furthest from the handle. The second lever is also rotatably connected to the turntable housing via the adjusting screw. The handle extends through the turntable housing. When the handle is turned, causing the second lever to rotate, the boss abuts against and pushes the first lever to rotate, thereby causing the light-shielding plate to cut out the light path so that the light emitted from the mercury lamp box enters the currently used fluorescent filter unit and shines through the second light-transmitting hole onto the specimen before being reflected back to the fluorescent filter unit and entering the human eye through the first light-transmitting hole. Alternatively, the light-shielding plate can cut into the light path to block the light emitted from the mercury lamp box from entering the currently used fluorescent filter unit.

[0010] Preferably, the fluorescent component further includes a polarization analyzer, a polarization generator, a first filter, a second filter, a first aperture adjustment unit, and a second aperture adjustment unit, which are sequentially built into the housing. The optical centers of the polarization generator, the first filter, the second filter, the first aperture adjustment unit, and the second aperture adjustment unit are coaxial. The optical center of the polarization analyzer is perpendicular to the optical center of the polarization generator. The second aperture adjustment unit is located close to the mercury lamp housing, and the light-shielding unit is located on the side of the polarization generator away from the mercury lamp housing.

[0011] Preferably, the fluorescent assembly further includes a first mirror group, a second mirror group, a third mirror group, and a fourth mirror group, all of which are built into the housing. The polarization analyzer, polarizing unit, first mirror group, first filter unit, second filter unit, second mirror group, first aperture adjustment unit, second aperture adjustment unit, third mirror group, and fourth mirror group are arranged in sequence and have coaxial optical centers. The fourth mirror group is also connected to a lamp box interface, and the mercury lamp box is connected to the housing through the lamp box interface.

[0012] Preferably, the polarization analyzer unit includes a first fixed base, a first cover plate, a first elastic positioning unit, and an analyzer. The first fixed base is connected to the housing and has a third light-transmitting hole coaxial with the first light-transmitting hole. The first fixed base also has a first groove. The first elastic positioning unit is built into the first groove. The first cover plate is connected to the first fixed base to form a first chamber. The analyzer is slidably inserted into the first chamber and has multiple first positioning slots arranged side by side. The first elastic positioning unit cooperates with one of the first positioning slots of the analyzer to limit the position, so as to realize the polarizer of the analyzer cutting into or out of the optical path.

[0013] Preferably, the turntable has several fourth light-transmitting holes, and the fluorescent filter units correspond one-to-one with the fourth light-transmitting holes. The turntable adjustment unit also includes a dovetail slide, a rotating unit, a positioning spring, and a rolling sleeve. The turntable has annularly distributed positioning grooves and rolling tracks corresponding to each of the fourth light-transmitting holes. The rotating unit includes a rotating base, several locking blocks, and a rotating shaft. The rotating base and the rotating shaft are rotatably connected. The rotating base has several dovetail guide rails parallel to the axis and evenly distributed in annular rings. The fluorescent filter units are slidably connected one-to-one with the dovetail guide rails. The locking blocks include a vertically connected first mounting base and a top plate. The first mounting base is slidably connected one-to-one with the dovetail guide rails and is connected to the dovetail guide rails. The tail guide rails are perpendicular to each other, and each first mounting seat is provided with at least one tapered groove parallel to the dovetail guide rail. The top plate achieves the abutment limit of the corresponding fluorescent color filter unit under the adjustment of the set screw passing through one of the tapered grooves. The rotating seat is connected to the turntable, and the dovetail slide is perpendicularly connected to the rotating shaft on the rotating unit and fixed on the turntable shell. The turntable shell is also provided with indicator labels corresponding to different fluorescent color filter units. The positioning spring is connected to the dovetail slide, and the roller is connected to the positioning spring. When the turntable rotates, the roller rolls along the rolling slide until it engages with the corresponding positioning groove for limit, thus completing the switching of the fluorescent color filter unit. The dovetail slide is slidably connected to the first fixed seat.

[0014] Preferably, the polarizing unit includes a slot assembly and a polarizer. The slot assembly includes a second fixed base, a second cover plate, and a second elastic positioning unit. The second fixed base is connected to the housing and has a second groove. The second elastic positioning unit is built into the second groove. The second fixed base is also connected to the second cover plate to form a second chamber. The polarizer is slidably inserted into the second chamber and has multiple second positioning slots arranged side by side. The second elastic positioning unit cooperates with one of the second positioning slots of the polarizer to limit the polarizer's movement, so as to realize that the polarizer's polarizing lens enters or exits the optical path.

[0015] Preferably, each elastic positioning unit includes a first clamping spring and a roller. Under the elastic force of the first clamping spring, the roller cooperates with a first positioning groove on the detector or a second positioning groove on the deflector for positioning.

[0016] Preferably, each aperture adjustment unit includes a mounting bracket, a third fixed seat, a blade mounting seat, a blade turntable, a second clamping spring, a pull rod, a sliding plate, a third cover plate, a tension spring, and multiple centering screws. The mounting bracket is connected to the housing, and the third fixed seat is connected to the third cover plate to form a third chamber. The blade mounting seat, blade turntable, second clamping spring, and sliding plate are all built into the third chamber. The blade turntable has several second slots evenly distributed around its circumference. The blade mounting seat also has several blades and several evenly distributed mounting holes around its circumference. Pins are provided on both sides of the blades. One side of the pins is rotatably connected to the mounting holes one by one, and the other side of the pins is slidably connected to the second slots one by one. The blade turntable is rotatably connected to the blade mounting base and has a boss. The blade mounting base has two parallel limiting grooves. The second clamping spring is arc-shaped and its two ends respectively abut against the two limiting grooves to clamp the slide plate. The tension spring is built into the third fixed base and its two ends are respectively connected to the third fixed base and the blade mounting base. The centering screw passes through the third fixed base and abuts against the blade mounting base. By adjusting the centering screw and under the action of the tension spring and the second clamping spring, the blade mounting base is centered. The slide plate has a first slot. The pull rod is connected to the slide plate. When the pull rod is pushed to drive the slide plate to move, the first slot drives the boss to move, thereby driving the blade turntable to rotate to adjust the light transmission.

[0017] Preferably, the screw post is vertically connected to the lower wall of the housing, the adjusting screw is vertically connected to the lower wall of the turntable housing, and the light-shielding unit also includes a torsion spring and a pressure rod connected to the lower wall of the housing. The torsion spring passes through the screw post and one end is connected to the housing, while the other end abuts against the first lever. When the second lever rotates, the first lever abuts against the column boss under the rebound action of the torsion spring, so that the light-shielding sheet cuts into the light path. The pressure rod is provided with a limiting groove, and the first lever passes through the limiting groove and is rotated and limited by the limiting groove.

[0018] Preferably, the housing includes a top cover and a base, with a first light-transmitting hole on the top cover and a second light-transmitting hole on the base.

[0019] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0020] This application allows users to freely switch to the desired fluorescence filter unit via a turntable adjustment unit. Users can install multiple fluorescence filter units of different wavelengths simultaneously as needed. The fluorescence filter unit and turntable adjustment unit are fixed with a dovetail design, making disassembly simple and allowing users to replace or maintain them themselves. Specific parameters of the fluorescence filter unit are displayed on a label on the turntable housing for easy selection. Furthermore, this device incorporates a light-shielding structure. By turning the handle on the turntable housing and using a linkage mechanism, the light-shielding plate can be moved in and out of the light path, improving light utilization, preventing rapid decay and quenching of fluorescent specimens, and extending the lifespan of fluorescent specimens. During use, there is no need to turn off the light source or remove the specimen from the light path. It also offers efficient space utilization, convenient operation, and facilitates rapid data collection. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the structure of the multi-band adjustable fluorescence device for the microscope of this utility model;

[0022] Figure 2 This is a schematic diagram of the internal structure of the multi-band adjustable fluorescence device for the microscope of this utility model;

[0023] Figure 3 This is a schematic diagram of the internal structure of the turntable adjustment unit of this utility model. Figure 1 ;

[0024] Figure 4 This is a schematic diagram of the internal structure of the turntable adjustment unit of this utility model. Figure 2 ;

[0025] Figure 5 This is a schematic diagram of the structure of the detection unit of this utility model;

[0026] Figure 6 This is a schematic diagram of the structure of the biasing unit of this utility model;

[0027] Figure 7 This is an exploded view of the deflection unit of this utility model;

[0028] Figure 8 This is a schematic diagram of the structure of the first aperture adjustment unit of this utility model;

[0029] Figure 9 This is an exploded view of the first aperture adjustment unit of this utility model;

[0030] Figure 10 This is a schematic diagram of the structure of the light-shielding unit of this utility model.

[0031] Explanation of reference numerals in the attached drawings: 1. Fluorescent component; 2. Mercury lamp housing; 11. Housing; 12. Turntable adjustment unit; 13. Shielding unit; 14. Analyzing unit; 15. Polarizing unit; 16. First filter unit; 17. Second filter unit; 18. First aperture adjustment unit; 19. Second aperture adjustment unit; 111. Top cover; 112. Base; 113. First lens group; 114. Second lens group; 115. Third lens group; 116. 121. Fourth lens group; 122. Turntable housing; 123. Dovetail slide; 124. Turntable; 125. Fluorescent filter unit; 126. Rotating unit; 127. Positioning spring; 131. Roller sleeve; 132. Light shield; 133. First lever; 134. Second lever; 135. Pressure lever; 136. Handle; 137. Screw post; 138. Adjusting screw; 141. Torsion spring; 142. First mounting base; 143. First cover plate; 143. First clamping spring; 144. Roller; 145. Detector; 151. Slot assembly; 152. Shifter; 181. Mounting bracket; 182. Third fixing seat; 183. Blade mounting seat; 184. Blade turntable; 185. Second clamping spring; 186. Pull rod; 187. Slide plate; 188. Third cover plate; 1161. Light box interface; 1231. Fourth light-transmitting hole; 1232. Positioning groove; 1233 1251 Rolling slide; 1252 Rotary seat; 1253 Locking block; 1254 Rotary shaft; 1255 Bearing; 1256 First pressure ring; 1257 Second pressure ring; 1331 Column boss; 1411 Third light-transmitting hole; 1451 First positioning groove; 1511 Second fixed seat; 1512 Second cover plate; 1521 Second positioning groove; 1831 Limiting groove; 1841 Boss; 1871 First slot. Detailed Implementation

[0032] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0033] It should be noted that when a component is referred to as being "connected" to another component, it can be directly connected to the other component or there may be an intervening component. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the application.

[0034] like Figure 1-6As shown, a multi-band adjustable fluorescence device for a microscope is mounted on the microscope body and includes a fluorescence component 1 and a mercury lamp housing 2. The fluorescence component 1 includes a housing 11, a turntable adjustment unit 12, and a light-shielding unit 13. The mercury lamp housing 2 is connected to the housing 11, wherein:

[0035] The housing 11 has a first light-transmitting hole on its upper wall and a second light-transmitting hole on its lower wall, and the first light-transmitting hole and the second light-transmitting hole are coaxial.

[0036] The turntable adjustment unit 12 includes a turntable housing 121, a turntable 123, and several fluorescent filter units 124. The turntable housing 121 is detachably connected to the housing 11 to form a cavity. The turntable 123 and the fluorescent filter units 124 are both built into the cavity. The fluorescent filter units 124 are distributed in a ring on the turntable 123. When the turntable 123 rotates, different fluorescent filter units 124 are cut into the light path.

[0037] The light-shielding unit 13, built into the cavity, includes a light-shielding plate 131, a first lever 132, a second lever 133, a handle 135, a screw post 136, and an adjusting screw 137. The light-shielding plate 131 is connected to the first lever 132, which is rotatably connected to the housing 11 via the screw post 136. The second lever 133 is connected to the handle 135 and has a vertically positioned boss 1331 at the end away from the handle 135. The second lever 133 is also rotatably connected to the turntable housing 121 via the adjusting screw 137. The handle 135 is also... Located outside the turntable housing 121, when the lever 135 is turned, causing the second lever 133 to rotate, the column boss 1331 abuts against and pushes the first lever 132 to rotate, thereby causing the light shield 131 to cut out the light path so that the light emitted from the mercury lamp light box 2 enters the currently used fluorescent filter unit 124, and after passing through the second light hole and illuminating the specimen, it is reflected back to the fluorescent filter unit 124 and enters the human eye through the first light hole, or the light shield 131 cuts into the light path to block the light emitted from the mercury lamp light box 2 from entering the currently used fluorescent filter unit 124.

[0038] like Figure 1 As shown, the first light-transmitting hole on the upper wall and the second light-transmitting hole on the lower wall of the housing 11 are coaxial and located in the optical path. The turntable housing 121 can be indirectly connected to the housing 11 for disassembly. The light-shielding unit 13 is installed at the bottom of the housing 11, and the handle 135 passes through the outside of the turntable housing 121. By adjusting the handle 135, the light-shielding plate 131 of the light-shielding unit 13 is controlled to enter or exit the optical path. The visible light emitted by the mercury lamp light box 2 covers multiple wavelengths, which can better meet the usage requirements. Furthermore, the mercury lamp light box 2 can also adopt the existing structure that can adjust the focal length of the internal light source and the center position of the light source.

[0039] The second lever 133 has a vertically provided post boss 1331 at the end away from the handle 135. The post boss 1331 always abuts against the first lever 132 and is located on the front side of the first lever 132 (the side away from the light shield 131). The first lever 132 includes two interconnected arc segments, which are hinged by a screw post 136. The arc segment that abuts against the post boss 1331 can be any shape. The second lever 133 can be any shape, such as a straight line or a V-shape. When the normal direction of the tangent point on the first lever 132 that contacts the column boss 1331 is collinear with the line connecting the column boss 1331 and the adjusting screw 137 and exceeds that angle, the light-shielding plate 131 completes the cutting of the light path and will not reset. In principle, the smaller the radius of the arc segment on the first lever 132 that abuts against the column boss 1331, the faster the switching. This light-shielding structure helps to improve space utilization and facilitates operation.

[0040] This device allows users to freely switch to the desired fluorescence filter unit via a turntable adjustment unit. Users can install multiple fluorescence filter units of different wavelengths simultaneously as needed. The fluorescence filter units and turntable adjustment unit are fixed with a dovetail design, making disassembly simple and allowing users to replace or maintain them themselves. Specific parameters of the fluorescence filter units are displayed on labels on the turntable housing for easy selection. Furthermore, the device incorporates a light-shielding structure. By turning the handle on the turntable housing and using a linkage mechanism, the light-shielding plate can be moved in and out of the light path, improving light utilization and preventing rapid attenuation and quenching of fluorescent specimens (fluorescent sections), thus extending the lifespan of the fluorescent specimens. During use, there is no need to turn off the light source or remove the specimen from the light path. It also offers efficient space utilization, convenient operation, and facilitates rapid data collection.

[0041] In one embodiment, the fluorescent component 1 further includes a polarization analyzer 14, a polarization generator 15, a first filter unit 16, a second filter unit 17, a first aperture adjustment unit 18, and a second aperture adjustment unit 19, which are sequentially built into the housing 11. The optical centers of the polarization generator 15, the first filter unit 16, the second filter unit 17, the first aperture adjustment unit 18, and the second aperture adjustment unit 19 are coaxial. The optical center of the polarization analyzer 14 is perpendicular to the optical center of the polarization generator 15. The second aperture adjustment unit 19 is located close to the mercury lamp housing 2. The light-shielding unit 13 is located on the side of the polarization generator 15 away from the mercury lamp housing 2.

[0042] like Figure 2As shown, the optical center of the polarizing unit 14 is perpendicular to the optical center of the polarizing unit 15. The polarizing unit 15, the first filter unit 16, the second filter unit 17, the first aperture adjustment unit 18, and the second aperture adjustment unit 19 are sequentially arranged inside the housing 11 with their optical centers coaxial. The turntable adjustment unit 12 is detachably connected to the polarizing unit 14 through the turntable housing 121. The first filter unit 16 and the second filter unit 17 are both existing technology structures, including a mounting base and a filter mounted on the mounting base, etc., which will not be described in detail here.

[0043] In one embodiment, the fluorescence assembly 1 further includes a first mirror group 113, a second mirror group 114, a third mirror group 115, and a fourth mirror group 116, all built into the housing 11. The analyzer unit 14, polarizer unit 15, first mirror group 113, first filter unit 16, second filter unit 17, second mirror group 114, first aperture adjustment unit 18, second aperture adjustment unit 19, third mirror group 115, and fourth mirror group 116 are arranged sequentially and coaxially. The fourth mirror group 116 is also connected to a lamp housing interface 1161, through which the mercury lamp lamp housing 2 is connected to the housing 11. The design of each mirror group helps to improve imaging quality, facilitating more accurate specimen observation.

[0044] In one embodiment, the polarization analyzer 14 includes a first fixed base 141, a first cover plate 142, a first elastic positioning unit, and an analyzer 145. The first fixed base 141 is connected to the housing 11 and has a third light-transmitting hole 1411 coaxial with the first light-transmitting hole. The first fixed base 141 also has a first groove. The first elastic positioning unit is built into the first groove. The first cover plate 142 is connected to the first fixed base 141 to form a first chamber. The analyzer 145 is slidably inserted into the first chamber and has multiple first positioning slots 1451 arranged side by side. The first elastic positioning unit cooperates with one of the first positioning slots 1451 of the analyzer 145 to limit the position, so as to realize the polarizer entry or exit of the analyzer 145 into the optical path.

[0045] When polarized light observation is enabled, the polarization analyzer 14 needs to be inserted into the optical path to achieve polarized light observation. In this embodiment, the polarization analyzer 145 can be a rotary polarization analyzer. Rotary polarization analyzers are well known to those skilled in the art and will not be described in detail here. When the polarized light from the polarization analyzer 14 and the polarization unit 15 is in an orthogonal state, the orthogonality state of the polarized light can be changed by turning the dial on the polarization analyzer 145. At the same time, the first elastic positioning unit on the first fixed base 141 will press the roller to form a locking engagement with one of the first positioning slots 1451 of the polarization analyzer 145 when the user inserts or slides the polarization analyzer 145 into or out of the optical path. The position on the housing 11 where the polarization analyzer 145 is installed is through, and the user can insert the polarization analyzer 14 into the optical path from the left or right side according to their usage habits.

[0046] In one embodiment, the turntable 123 has a plurality of fourth light-transmitting holes 1231, and the fluorescent color-filtering unit 124 corresponds one-to-one with the fourth light-transmitting hole 1231. The turntable adjustment unit 12 also includes a dovetail slide 122, a rotating unit 125, a positioning spring 126, and a rolling sleeve 127. The turntable 123 has annularly distributed positioning grooves 1232 and rolling slides 1233 at the positions corresponding to each fourth light-transmitting hole 1231. The rotating unit 125 includes a rotating base 1251, a plurality of locking blocks 1252, and a rotating shaft 1253. The rotating base 1251 and the rotating shaft 1253 are rotatably connected. The rotating base 1251 has a plurality of dovetail guide rails parallel to the axis and annularly distributed. The fluorescent color-filtering unit 124 is slidably connected one-to-one with the dovetail guide rails. The locking block 1252 includes a first mounting base and a top plate vertically connected. The first mounting base and the dovetail guide rail are connected one-to-one with the top plate. A corresponding sliding connection is provided and perpendicular to the dovetail guide rail. Each first mounting seat is also provided with at least one conical groove parallel to the dovetail guide rail. The top plate achieves the abutment and limit of the corresponding fluorescent color filter unit 124 under the adjustment of the set screw passing through one of its conical grooves. The rotating seat 1251 is connected to the turntable 123. The dovetail slide 122 is perpendicularly connected to the rotating shaft 1253 on the rotating unit 125 and fixed on the turntable shell 121. The turntable shell 121 is also provided with indicator labels corresponding to different fluorescent color filter units 124. The positioning spring 126 is connected to the dovetail slide 122. The roller sleeve 127 is connected to the positioning spring. When the turntable 123 rotates, the roller sleeve 127 rolls along the rolling slide 1233 until it cooperates with the corresponding positioning groove 1232 for limit, completing the switching of the fluorescent color filter unit 124. The dovetail slide 122 is slidably connected to the first fixed seat 141.

[0047] The turntable adjustment unit 12 is slidably connected to the first fixed base 141 via a dovetail slide 122. When the turntable 123 is rotated, the entry and exit optical paths of the fluorescent color filter unit 124 can be switched. The rotating unit 125 is fixedly connected to the turntable 123 via a threaded hole on the rotating base 1251. The dovetail slide 122 is fixedly connected to the rotating shaft 1253 on the rotating unit 125. The turntable housing 121 is fixed on the dovetail slide 122. The fluorescent color filter unit 124 is slidably connected to the dovetail guide rail on the rotating base 1251. At the same time, a locking block 1252 is installed in the rotating base 1251 at the position corresponding to the dovetail guide rail. The locking block 1252 is tightened by the set screw, thereby locking the fluorescent color filter unit 124. Loosening the set screw allows for quick disassembly of the fluorescent color filter unit 124. This structure is simple and facilitates the replacement and disassembly of fluorescent color filters 124 of different wavelengths.

[0048] A positioning spring 126 is installed on the dovetail slide 122. One end of the positioning spring 126 is provided with a roller sleeve 127. When the turntable 123 is rotated, the roller sleeve 127 will roll on the rolling slide 1233 of the turntable 123 until it engages with the corresponding positioning groove 1232. The rolling contact method reduces friction and improves the smoothness of the turntable 123 when it rotates.

[0049] The rotating unit 125 may also include several bearings 1254, a first pressure ring 1255 and a second pressure ring 1256. The bearings 1254, the first pressure ring 1255 and the second pressure ring 1256 are all coaxially mounted on the rotating shaft 1253. The rotating seat 1251 achieves relative rotation with the rotating seat 1251 through the bearings 1254 and uses the first pressure ring 1255 and the second pressure ring 1256 to press the bearings 1254 in sequence for axial positioning. The second pressure ring 1256 can prevent the first pressure ring 1255 from loosening during use. The bearings 1254 may be deep groove ball bearings, etc.

[0050] In one embodiment, the polarizing unit 15 includes a slot assembly 151 and a polarizer 152. The slot assembly 151 includes a second fixing seat 1511, a second cover plate 1512, and a second elastic positioning unit. The second fixing seat 1511 is connected to the housing 11 and has a second groove. The second elastic positioning unit is built into the second groove, and the second fixing seat 1511 is also connected to the second cover plate 1512 to form a second chamber. The polarizer 152 is slidably inserted into the second chamber and has a plurality of second positioning slots 1521 arranged side by side. The second elastic positioning unit cooperates with one of the second positioning slots 1521 of the polarizer 152 to limit the polarizer 152 so as to realize the polarizer 152's polarizing mirror cutting into or out of the optical path.

[0051] The polarizing unit 15 can be used in conjunction with the polarizing unit 14 to achieve polarized light observation. That is, when performing polarized light observation, both the polarizer 152 and the analyzer 145 need to be inserted into the optical path (the analyzer of the analyzer 145 and the polarizer of the polarizer 152 are in the optical path). The polarized light observation is achieved by adjusting the angle of the analyzer by turning the handwheel of the analyzer 145.

[0052] In one embodiment, each elastic positioning unit includes a first clamping spring 143 and a roller 144. Under the elastic force of the first clamping spring 143, the roller 144 engages with a first positioning groove 1451 on the detector 145 or a second positioning groove 1521 on the deflector 152 for positioning. This facilitates quick adjustment to achieve accurate positioning.

[0053] In one embodiment, each aperture adjustment unit includes a mounting bracket 181, a third fixing seat 182, a blade mounting seat 183, a blade turntable 184, a second clamping spring 185, a pull rod 186, a sliding plate 187, a third cover plate 188, a tension spring, and multiple centering screws. The mounting bracket 181 is connected to the housing 11, and the third fixing seat 182 is connected to the third cover plate 188 to form a third chamber. The blade mounting seat 183, blade turntable 184, second clamping spring 185, and sliding plate 187 are all built into the third chamber. The blade turntable 184 has several second slots evenly distributed around its circumference. The blade mounting seat 183 also has several blades and several mounting holes evenly distributed around its circumference. Pins are provided on both sides of the blades. One pin is rotatably connected to the mounting hole, and the other pin is slidably engaged with the second slot. The blade turntable 184 and the blade... The blade mounting base 183 is rotatably connected and has a boss 1841. The blade mounting base 183 has two parallel limiting grooves 1831. The second clamping spring 185 is arc-shaped and its two ends respectively abut against the two limiting grooves 1831 to clamp the slide plate 187. The tension spring is built into the third fixed base 182 and its two ends are respectively connected to the third fixed base 182 and the blade mounting base 183. The centering screw passes through the third fixed base 182 and abuts against the blade mounting base 183. By adjusting the centering screw and under the action of the tension spring and the second clamping spring 185, the blade mounting base 183 is centered. The slide plate 187 has a first slot 1871. The pull rod 186 is connected to the slide plate 187. When the pull rod 186 is pushed to drive the slide plate 187 to move, the first slot 1871 drives the boss 1841 to move, thereby driving the blade turntable 184 to rotate to adjust the light transmission.

[0054] like Figure 8 , 9 As shown, the aperture adjustment unit can be centered before use. For example, two centering screws are vertically installed on the right side wall of the third fixed seat 182. The blade mounting seat 183 is adjusted to be centered in the optical path by the tension of the two centering screws and the tension of the tension spring, in conjunction with the second clamping spring 185. During the adjustment process, the luminous flux of the aperture adjustment unit needs to be adjusted to the minimum first. The position of the aperture image center is observed by visual observation, and the aperture image is adjusted to the center of the field of view by the centering screws to complete the centering process.

[0055] The third fixed seat 182 and the blade mounting seat 183 are connected by a tension spring to apply tension. The slide plate 187 is provided with two first slots 1871 (one of which is redundant for easy position adjustment). The pull rod 186 is connected to the slide plate 187 by screws. When the pull rod 186 is pushed, the first slot 1871 on the slide plate 187 will drive the boss 1841, thereby making the blade turntable 184 rotate. The first aperture adjustment unit 18 and the second aperture adjustment unit 19 operate on the same principle. If other structures are the same, only by changing the position of the slide plate 187, the boss 1841 will cooperate with the first slot 1871 in different positions, so that the position of the pull rod 186 is on the left or right side to accommodate different users.

[0056] In one embodiment, the screw post 136 is vertically connected to the lower wall of the housing 11, and the adjusting screw 137 is vertically connected to the lower wall of the turntable housing 121. The light-shielding unit 13 also includes a torsion spring 138 and a pressure rod 134 connected to the lower wall of the housing 11. The torsion spring 138 passes through the screw post 136 and one end is connected to the housing 11, while the other end abuts against the first lever 132. When the second lever 133 rotates, the first lever 132 abuts against the column boss 1331 under the rebound action of the torsion spring 138, so that the light-shielding sheet 131 cuts into the light path. The pressure rod 134 is provided with a limiting groove, and the first lever 132 passes through the limiting groove and is rotated and limited by the limiting groove.

[0057] The housing 11 is equipped with a pressure rod 134, which includes a limiting boss and a pressure plate. The limiting boss and the pressure plate are connected to form a limiting groove. The limiting boss can be separately formed from or integrally formed with the housing 11. The first lever 132 is located in the limiting groove, which can limit the first lever 132 and prevent the first lever 132 from moving in the vertical direction. When the second lever 133 is manually rotated, due to the rebound of the torsion spring 138, the first lever 132 will press tightly against the column boss 1331 on the second lever 133. As it is manually rotated to the corresponding position, it slowly cuts into the optical path.

[0058] In one embodiment, the housing 11 includes a top cover 111 and a base 112, with a first light-transmitting hole on the top cover 111 and a second light-transmitting hole on the base 112.

[0059] Working principle:

[0060] The multi-band adjustable fluorescence device is mounted on the microscope body. The mercury lamp box is powered on, and the corresponding fluorescence filter unit 124 is inserted into the optical path. The handle 135 is moved to cut out the light-blocking plate 131, and the field diaphragm is adjusted to its minimum. A clear image of the diaphragm will then appear in the field of view. The diaphragm image is then centered using the centering screw. When observing a specimen, to collect data at different wavelengths, the dial 123 can be rotated to switch between different fluorescence filter units 124, enabling observation at different wavelengths. To pause observation, simply move the handle to insert the light-blocking plate 131 into the optical path to prevent attenuation and quenching of the specimen's fluorescence. After the mercury lamp light box 2 is turned on, it emits a beam of visible light. The light passes sequentially through the fourth mirror group 116, the third mirror group 115, the second aperture adjustment unit 19 (aperture aperture), the first aperture adjustment unit 18 (field aperture), the second mirror group 114, the second filter unit 17, the first filter unit 16, the first mirror group 113, and the polarizing unit 15, passing over the light shield 131 and reaching the fluorescence filter unit 124. The excitation plate of the fluorescence filter unit 124 only allows light of a specific wavelength to pass through the excitation plate, which is then reflected by the two-way beam splitter and illuminates the specimen through the fluorescence objective of the microscope. The fluorophores of the specimen are activated and emit long-wavelength fluorescence. At this time, the light passes through the fluorescence objective, through the two-way beam splitter, and reaches the cutoff plate of the fluorescence filter unit 124. At this time, only light of a specific wavelength passes through the cutoff plate, and finally the fluorescence visible to the naked eye is captured.

[0061] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0062] The embodiments described above are merely specific and detailed examples of the embodiments described in this application, and should not be construed as limiting the scope of this patent application. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims.

Claims

1. A multi-band adjustable fluorescence device for a microscope, mounted on the microscope body, characterized in that: The microscope multi-band adjustable fluorescence device includes a fluorescence component (1) and a mercury lamp housing (2). The fluorescence component (1) includes a housing (11), a turntable adjustment unit (12), and a light-shielding unit (13). The mercury lamp housing (2) is connected to the housing (11). The housing (11) has a first light-transmitting hole on its upper wall and a second light-transmitting hole on its lower wall, and the first light-transmitting hole and the second light-transmitting hole are coaxial. The turntable adjustment unit (12) includes a turntable shell (121), a turntable (123), and a plurality of fluorescent color filter units (124). The turntable shell (121) is detachably connected to the shell (11) to form a cavity. The turntable (123) and the fluorescent color filter units (124) are both built into the cavity. The fluorescent color filter units (124) are arranged in a ring on the turntable (123). When the turntable (123) rotates, different fluorescent color filter units (124) are cut into the light path. The light-shielding unit (13), built into the cavity, includes a light-shielding plate (131), a first lever (132), a second lever (133), a handle (135), a screw post (136), and an adjusting screw (137). The light-shielding plate (131) is connected to the first lever (132), and the first lever (132) is rotatably connected to the housing (11) via the screw post (136). The second lever (133) is connected to the handle (135) and has a vertically provided column protrusion (1331) at one end away from the handle (135). The second lever (133) is also rotatably connected to the turntable housing (121) via the adjusting screw (137). The handle (135) is rotatably connected to the turntable housing (121). 35) It is also installed outside the turntable housing (121). When the handle (135) is turned to drive the second lever (133) to rotate, the column boss (1331) pushes the first lever (132) to rotate, thereby causing the light shield (131) to cut out the light path so that the light emitted by the mercury lamp box (2) enters the currently used fluorescent filter unit (124) and shines on the specimen through the second light hole and is reflected back to the fluorescent filter unit (124) and enters the human eye through the first light hole, or the light shield (131) cuts into the light path to block the light emitted by the mercury lamp box (2) from entering the currently used fluorescent filter unit (124).

2. The microscope multi-band tunable fluorescence device as described in claim 1, characterized in that: The fluorescent component (1) further includes a polarization analyzer (14), a polarization generator (15), a first filter unit (16), a second filter unit (17), a first aperture adjustment unit (18), and a second aperture adjustment unit (19) sequentially built into the housing (11). The optical centers of the polarization generator (15), the first filter unit (16), the second filter unit (17), the first aperture adjustment unit (18), and the second aperture adjustment unit (19) are coaxial. The optical center of the polarization analyzer (14) is perpendicular to the optical center of the polarization generator (15). The second aperture adjustment unit (19) is located close to the mercury lamp box (2). The light-shielding unit (13) is located on the side of the polarization generator (15) away from the mercury lamp box (2).

3. The microscope multi-band tunable fluorescence device as described in claim 2, characterized in that: The fluorescence assembly (1) also includes a first mirror group (113), a second mirror group (114), a third mirror group (115), and a fourth mirror group (116), all of which are built into the housing (11). The polarization analyzer (14), polarization unit (15), first mirror group (113), first filter unit (16), second filter unit (17), second mirror group (114), first aperture adjustment unit (18), second aperture adjustment unit (19), third mirror group (115), and fourth mirror group (116) are arranged in sequence and have coaxial optical centers. The fourth mirror group (116) is also connected to a lamp box interface (1161). The mercury lamp lamp box (2) is connected to the housing (11) through the lamp box interface (1161).

4. The microscope multi-band adjustable fluorescence device as described in claim 2, characterized in that: The polarization analyzer (14) includes a first fixed base (141), a first cover plate (142), a first elastic positioning unit, and an analyzer (145). The first fixed base (141) is connected to the housing (11) and has a third light-transmitting hole (1411) coaxial with the first light-transmitting hole. The first fixed base (141) also has a first groove. The first elastic positioning unit is built into the first groove. The first cover plate (142) is connected to the first fixed base (141) and forms a first chamber. The analyzer (145) is slidably inserted into the first chamber and has multiple first positioning slots (1451) arranged side by side. The first elastic positioning unit cooperates with one of the first positioning slots (1451) of the analyzer (145) to limit the position, so as to realize the polarizer of the analyzer (145) cutting into or out of the optical path.

5. The microscope multi-band tunable fluorescence device as described in claim 4, characterized in that: The turntable (123) has several fourth light-transmitting holes (1231), and the fluorescent color filter unit (124) corresponds one-to-one with the fourth light-transmitting hole (1231). The turntable adjustment unit (12) also includes a dovetail slide (122), a rotating unit (125), a positioning spring (126), and a rolling sleeve (127). The turntable (123) has annularly distributed positioning grooves (1232) and rolling tracks (1233) corresponding to the positions of each of the fourth light-transmitting holes (1231). The unit (125) includes a rotating base (1251), several locking blocks (1252), and a rotating shaft (1253). The rotating base (1251) is rotatably connected to the rotating shaft (1253). The rotating base (1251) is provided with several dovetail guide rails that are parallel to the axis and evenly distributed in a ring. The fluorescent color filter unit (124) is slidably connected to the dovetail guide rails one by one. The locking block (1252) includes a first mounting base and a top plate that are vertically connected. The first mounting base is slidably connected to the dovetail guide rails one by one and is connected to the top plate. The dovetail guide rails are perpendicular to each other, and each of the first mounting seats is provided with at least one conical groove parallel to the dovetail guide rail. The top plate is adjusted by the set screw passing through one of the conical grooves to achieve the abutment and limiting position of the corresponding fluorescent color filter unit (124). The rotating seat (1251) is connected to the turntable (123). The dovetail slide (122) is perpendicularly connected to the rotating shaft (1253) on the rotating unit (125) and fixed on the turntable shell (121). The turntable shell (121) The upper part is also provided with indicator labels corresponding to different fluorescent color filter units (124). The positioning spring (126) is connected to the dovetail slide (122), and the roller sleeve (127) is connected to the positioning spring. When the turntable (123) rotates, the roller sleeve (127) rolls along the rolling slide (1233) until it engages with the corresponding positioning groove (1232) to limit the position, thereby completing the switching of the fluorescent color filter unit (124). The dovetail slide (122) is slidably connected to the first fixed seat (141).

6. The microscope multi-band tunable fluorescence device as described in claim 4, characterized in that: The polarizing unit (15) includes a slot assembly (151) and a polarizer (152). The slot assembly (151) includes a second fixing seat (1511), a second cover plate (1512), and a second elastic positioning unit. The second fixing seat (1511) is connected to the housing (11) and has a second groove. The second elastic positioning unit is built into the second groove. The second fixing seat (1511) is also connected to the second cover plate (1512) to form a second chamber. The polarizer (152) is slidably inserted into the second chamber and has multiple second positioning slots (1521) arranged side by side. The second elastic positioning unit cooperates with one of the second positioning slots (1521) of the polarizer (152) to limit the polarizer's entry or exit from the optical path.

7. The microscope multi-band tunable fluorescence device as described in claim 6, characterized in that: Each of the elastic positioning units includes a first pressing spring (143) and a roller (144). The roller (144) is engaged with one of the first positioning grooves (1451) on the detector (145) or one of the second positioning grooves (1521) on the deflector (152) under the elastic force of the first pressing spring (143).

8. The microscope multi-band tunable fluorescence device as described in claim 2, characterized in that: Each of the aforementioned aperture adjustment units includes a mounting bracket (181), a third fixing seat (182), a blade mounting seat (183), a blade turntable (184), a second clamping spring (185), a pull rod (186), a sliding plate (187), a third cover plate (188), a tension spring, and multiple centering screws. The mounting bracket (181) is connected to the housing (11), the third fixing seat (182) is connected to the third cover plate (188) and forms a third chamber, and the blade mounting seat (183) and the blade turntable (184) are connected to the housing (185). 4) The second clamping spring (185) and the sliding plate (187) are both built into the third chamber. The blade turntable (184) has several second slots evenly distributed around its circumference. The blade mounting base (183) also has several blades and several mounting holes evenly distributed around its circumference. The blades are provided with pins on both sides. One side of the pin is rotatably connected to the mounting hole, and the other side of the pin is slidably engaged with the second slot. The blade turntable (184) is rotatably connected to the blade mounting base (183) and is provided with... The boss (1841) and the blade mounting base (183) are provided with two parallel limiting grooves (1831). The second clamping spring (185) is arc-shaped and its two ends respectively abut against the two limiting grooves (1831) to clamp the slide plate (187). The tension spring is built into the third fixing base (182) and its two ends are respectively connected to the third fixing base (182) and the blade mounting base (183). The centering screw passes through the third fixing base (182) and abuts against the blade mounting base (1841). 183), by adjusting the centering screw and under the action of the tension spring and the second clamping spring (185), the blade mounting seat (183) is centered. The slide plate (187) is provided with a first slot (1871). The pull rod (186) is connected to the slide plate (187). When the pull rod (186) is pushed to drive the slide plate (187) to move, the first slot (1871) drives the boss (1841) to move, thereby driving the blade turntable (184) to rotate to adjust the light transmission.

9. The microscope multi-band tunable fluorescence device as described in claim 1, characterized in that: The screw post (136) is vertically connected to the lower wall of the housing (11), and the adjusting screw (137) is vertically connected to the lower wall of the turntable housing (121). The light-shielding unit (13) also includes a torsion spring (138) and a pressure rod (134) connected to the lower wall of the housing (11). The torsion spring (138) passes through the screw post (136) and one end is connected to the housing (11), while the other end abuts against the first lever (132). When the second lever (133) rotates, the first lever (132) abuts against the column boss (1331) under the rebound action of the torsion spring (138) to realize that the light-shielding plate (131) cuts into the light path. The pressure rod (134) is provided with a limiting groove. The first lever (132) passes through the limiting groove and is rotated and limited by the limiting groove.

10. The microscope multi-band tunable fluorescence device according to any one of claims 1 to 9, characterized in that: The housing (11) includes a top cover (111) and a base (112), with the first light-transmitting hole on the top cover (111) and the second light-transmitting hole on the base (112).

Images