Protein separation and purification device

Abstract

The utility model discloses a protein separation and purification device which comprises a bottom plate and a chromatographic column vertically arranged on the bottom plate, a support frame with one end fixedly connected with the bottom plate is fixedly arranged outside the chromatographic column, and the bottom of the chromatographic column is communicated with a liquid outlet pipe penetrating through the lower part of the bottom plate; an annular sealing seat is fixedly arranged at the top of the bottom plate and located outside the chromatographic column and the supporting frame, a sealing cover with one end extending into the annular sealing seat is inserted into the top of the annular sealing seat, and a pressurizing assembly is arranged on the sealing cover and used for increasing the air pressure in the sealing cover; an adjusting assembly fixedly connected with the sealing cover is arranged on the bottom plate and used for adjusting the sealing cover to ascend or descend. According to the utility model, the pressurizing assembly is arranged and can be used for adjusting the air pressure of the environment where the chromatographic column is located, and after the air pressure of the environment where the chromatographic column is located is adjusted according to the requirements of a user, the working efficiency of the chromatographic column can be properly improved, and the time is saved.

Description

TECHNICAL FIELD

[0001] The utility model relates to protein separation and purification equipment field, especially in a kind of protein separation and purification device. BACKGROUND

[0002] Chromatography column is the main body in gel chromatography technology, generally glass tube or organic glass tube, according to the distribution coefficient of each component in sample mixture in stationary phase and mobile phase, thereby separating a kind of chromatography.

[0003] Protein separation and purification can use chromatography column to separate and purify, under conventional air pressure environment, the efficiency of chromatography column is lower when separating and purifying, therefore a kind of protein separation and purification device is presented. UTILITY MODEL CONTENT

[0004] The technical problem to be solved by the utility model is to overcome the defects of prior art, provide a kind of protein separation and purification device, the air pressure of chromatography column can be adjusted, and the working efficiency of chromatography column is improved.

[0005] In order to solve the above technical problems, the utility model provides the following technical scheme:

[0006] The utility model relates to a kind of protein separation and purification device, including bottom plate and vertically arranged chromatography column in bottom plate setting, the outside of chromatography column is fixedly arranged with the support frame of one end and bottom plate fixed connection, the bottom of chromatography column is communicated with the liquid outlet pipe that penetrates to bottom plate below;

[0007] The top of bottom plate and located the outside of chromatography column and support frame are fixedly provided with annular sealing seat, the top of annular sealing seat is inserted with the sealing cover of one end and extends into annular sealing seat, the sealing cover is equipped with pressurizing assembly, for increasing the air pressure in sealing cover;

[0008] The bottom plate is equipped with the adjusting assembly of sealing cover fixed connection, for adjusting the sealing cover ascending or descending.

[0009] As a preferred technical scheme of the utility model, the top of annular sealing seat is provided with annular groove for the insertion of sealing cover, and the height of sealing cover is twenty centimeters higher than the height of chromatography column.

[0010] As a preferred technical scheme of the utility model, the top of bottom plate is equipped with sealing washer at the connection place of liquid outlet pipe.

[0011] As a preferred technical scheme of the utility model, the bottom of bottom plate is fixedly provided with support pad on left and right sides, and the height of support pad is greater than the length of liquid outlet pipe extending below bottom plate.

[0012] As a preferred technical scheme of the utility model, the pressurizing assembly comprises a driving push rod, a positioning frame and a piston plate, wherein,

[0013] The driving push rod is fixedly arranged at the top of the sealing cover, and its output end penetrates into the inside of the sealing cover;

[0014] The positioning frame is fixedly arranged outside the driving push rod, and one end thereof is fixedly connected with the sealing cover;

[0015] The piston plate is fixedly arranged at the output end of the driving push rod, and its outside is in contact with the inside of the sealing cover.

[0016] As a preferred technical scheme of the utility model, the front surface of the sealing cover is embedded with an observation window, the front surface of the sealing cover and located at the right side of the observation window is sprayed with a scale ruler, and the starting position of the scale ruler and the lower end of the starting position of the piston plate are on the same horizontal line.

[0017] As a preferred technical scheme of the utility model, the adjusting assembly comprises an electric push rod and a connecting plate, the electric push rod is fixedly arranged at the bottom of the bottom plate on the left and right sides, and the connecting plate is fixedly arranged at the output end of the electric push rod, and the other end thereof is fixedly connected with the sealing cover.

[0018] Compared with the prior art, the utility model has the beneficial effects as follows:

[0019] The utility model discloses a pressurizing assembly can adjust the air pressure of the environment where the chromatography column is located, and after adjusting the air pressure of the environment where the chromatography column is located according to the requirement of the user, the working efficiency of the chromatography column can be properly improved, and time is saved. DRAWINGS

[0020] The drawings are used to provide further understanding of the utility model and constitute a part of the specification, are used together with the embodiments of the utility model to explain the utility model, and do not constitute the limitation to the utility model.

[0021] Fig. 1 It is the sectional view of the utility model;

[0022] Fig. 2 It is the front view of the utility model;

[0023] Fig. 3 It is the embodiment schematic view of the utility model after the sealing cover is moved up;

[0024] In the drawing: 1, bottom plate; 2, chromatography column; 3, support frame; 4, liquid outlet pipe; 5, annular sealing seat; 6, sealing cover; 61, observation window; 62, scale ruler; 7, driving push rod; 8, positioning frame; 9, piston plate; 10, electric push rod; 11, connecting plate; 12, supporting pad. DETAILED DESCRIPTION

[0025] The preferred embodiments of the present application are described below in conjunction with the accompanying drawings, in which it is understood that the preferred embodiments described below are merely intended to illustrate and explain the present application, and are not intended to limit the present application.

[0026] The same reference numerals in the drawings refer to the same components throughout.

[0027] Example 1

[0028] As Figs. 1-3 shown, the present application provides a protein separation and purification device, which comprises a bottom plate 1 and a chromatography column 2 vertically arranged on the bottom plate 1, the chromatography column 2 is externally fixedly provided with a support frame 3 fixedly connected with the bottom plate 1 at one end, the bottom of the chromatography column 2 is communicated with a liquid outlet pipe 4 penetrating below the bottom plate 1, the top of the bottom plate 1 is provided with a sealing gasket at the connection with the liquid outlet pipe 4, the top of the bottom plate 1 and outside the chromatography column 2 and the support frame 3 is externally fixedly provided with an annular sealing seat 5, the top of the annular sealing seat 5 is inserted with a sealing cover 6 extending into the inside of the annular sealing seat 5 at one end, the sealing cover 6 is provided with a pressurizing assembly, which is used to increase the air pressure in the sealing cover 6, the pressurizing assembly comprises a driving push rod 7, a positioning frame 8 and a piston plate 9, wherein the driving push rod 7 is fixedly arranged at the top of the sealing cover 6, and the output end thereof penetrates into the inside of the sealing cover 6; the positioning frame 8 is fixedly arranged outside the driving push rod 7, and one end thereof is fixedly connected with the sealing cover 6; the piston plate 9 is fixedly arranged at the output end of the driving push rod 7, and the outside thereof is in contact with the inside of the sealing cover 6.

[0029] The bottom plate 1 is provided with an adjusting assembly fixedly connected with the sealing cover 6, which is used to adjust the rising or falling of the sealing cover 6, the adjusting assembly comprises an electric push rod 10 and a linking plate 11, the electric push rod 10 is fixedly arranged at the bottom of the bottom plate 1 on both sides, and the linking plate 11 is fixedly arranged at the output end of the electric push rod 10, and the other end thereof is fixedly connected with the sealing cover 6.

[0030] In addition, the top of the annular sealing seat 5 is provided with an annular groove for inserting the sealing cover 6, the height of the sealing cover 6 is twenty centimeters higher than the height of the chromatography column 2, and the bottom of the bottom plate 1 is fixedly provided with a support pad 12 on both sides, and the height of the support pad 12 is greater than the length of the liquid outlet pipe 4 extending below the bottom plate 1.

[0031] It should be noted that the front of the sealing cover 6 is embedded with an observation window 61, and the front of the sealing cover 6 and the right side of the observation window 61 are sprayed with a scale 62, the starting position of the scale 62 and the lower end of the starting position of the piston plate 9 are on the same horizontal line, the starting position of the piston plate 9 refers to the uppermost of the moving range of the piston plate 9, and when the device is manufactured, the air pressure monitoring device is placed in the sealing cover 6, and when the piston plate 9 moves downward and the air pressure changes, the scale 62 is counted, which is convenient for the user to judge the current air pressure of the sealing cover 6.

[0032] In particular, the regulation of air pressure presents the following risks, and therefore requires air pressure control according to specific conditions, the risks are as follows:

[0033] Resolution reduction, pressurization may cause resolution reduction, especially when separating complex samples, because faster flow rates may not allow enough time for effective partitioning and adsorption-desorption processes, resulting in peak overlap;

[0034] Damage to column media, excessive pressure may cause damage to the media in the chromatographic column, such as resin, silica gel, etc., resulting in structural damage or pore blockage. Long-term high-pressure operation may reduce the service life of the column;

[0035] Sample damage, some proteins may undergo conformational changes under high pressure, or be subjected to degradation under excessive pressure, excessive pressure may affect the stability and activity of proteins, especially for some sensitive proteins.

[0036] In summary, the utility model discloses a pressurizing assembly can adjust the air pressure of the environment where the chromatographic column 2 is located, after adjusting the air pressure of the environment where the chromatographic column 2 is located according to the demand of the user, the working efficiency of the chromatographic column 2 can be appropriately improved, and time is saved.

[0037] Finally, it should be noted that: the above only for the preferred embodiments of the utility model have been described, and are not used to limit the utility model, although the utility model is described in detail with reference to the foregoing embodiments, for the person skilled in the art, it still can modify the technical scheme recorded in the foregoing each embodiment, or equivalent replacement to part of technical features. Any modification, equivalent replacement, improvement, etc. within the spirit and principles of the utility model should be included in the protection scope of the utility model.

Claims

1. A protein separation and purification device, characterized by, The application relates to a chromatographic column device, which comprises a bottom plate (1) and a chromatographic column (2) vertically arranged on the bottom plate (1), wherein the chromatographic column (2) is externally fixedly provided with a support frame (3) with one end fixedly connected with the bottom plate (1), and the bottom of the chromatographic column (2) is communicated with a liquid outlet pipe (4) penetrating to the lower side of the bottom plate (1). An annular sealing seat (5) is fixedly arranged on the top of the bottom plate (1) and externally fixed to the chromatographic column (2) and the support frame (3), a sealing cover (6) with one end extending into the annular sealing seat (5) is inserted into the top of the annular sealing seat (5), and a pressurizing assembly is arranged on the sealing cover (6) and used for increasing the air pressure in the sealing cover (6). An adjusting assembly is fixedly connected with the sealing cover (6) on the bottom plate (1) and used for adjusting the ascending or descending of the sealing cover (6).

2. The protein separation and purification device according to claim 1, characterized in that, An annular groove for inserting the sealing cover (6) is formed in the top of the annular sealing seat (5), and the height of the sealing cover (6) is 20 cm higher than the height of the chromatographic column (2).

3. The protein separation and purification device according to claim 1, characterized in that, A sealing washer is arranged at the connecting position between the top of the bottom plate (1) and the liquid outlet pipe (4).

4. The protein separation and purification device according to claim 1, characterized in that, Support pads (12) are fixedly arranged on the left and right sides of the bottom of the bottom plate (1), and the height of the support pads (12) is greater than the length of the liquid outlet pipe (4) extending to the lower side of the bottom plate (1).

5. The protein separation and purification device according to claim 1, wherein The pressurizing assembly comprises a driving push rod (7), a positioning frame (8) and a piston plate (9), wherein the driving push rod (7) is fixedly arranged on the top of the sealing cover (6) and has an output end penetrating into the interior of the sealing cover (6), the positioning frame (8) is fixedly arranged on the exterior of the driving push rod (7) and has one end fixedly connected with the sealing cover (6), and the piston plate (9) is fixedly arranged on the output end of the driving push rod (7) and has an exterior in contact with the interior of the sealing cover (6). An observation window (61) is embedded on the front surface of the sealing cover (6), a scale (62) is sprayed on the front surface of the sealing cover (6) and located on the right side of the observation window (61), and the starting position of the scale (62) is on the same horizontal line with the lower end of the starting position of the piston plate (9). The adjusting assembly comprises an electric push rod (10) and a linking plate (11), the electric push rod (10) is fixedly arranged on the bottom of the bottom plate (1) and on the left and right sides, and the linking plate (11) is fixedly arranged on the output end of the electric push rod (10) and has the other end fixedly connected with the sealing cover (6). ​ 6. A protein separation and purification device according to claim 5, wherein, ​ 7. The protein separation and purification device according to claim 1, wherein ​

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