Single cell microinjection device

Abstract

The utility model belongs to the technical field of microinjection, and particularly relates to a single cell microinjection device which comprises a rotating shaft, a mounting frame is mounted at one end of the rotating shaft, rotating shafts are rotatably mounted on two sides of the mounting frame in a penetrating manner, a locking screw is rotatably mounted in one end of a side plate, and a gear groove is mounted at the other end of the rotating shaft. A clamping block is assembled at the bottom end of the limiting rod, and an extension spring is fixedly connected between the limiting block and the supporting plate. A limiting block is lifted upwards, a limiting rod moves upwards, an extension spring is stretched, a clamping block is separated from a gear groove, a rotating shaft drives a mounting frame to rotate, the angle of the injector in the X-axis direction is changed, the angle of the injector in the Y-axis direction is adjusted, a locking screw releases fixation of a rotating shaft, and the injector is rotated through rotation of the rotating shaft; therefore, the angle of the injector can be adjusted at will, the injector can be aligned with target cells more flexibly in the micro-injection process, and the injection effect is improved.

Description

Technical Field

[0001] This invention relates to the field of microinjection technology, specifically a single-cell microinjection device. Background Technology

[0002] A single cell refers to an organism consisting of only one cell. In the study of single-cell organisms in plant cells, specific gene fragments are introduced into single cells through microinjection to observe gene expression within the single cell and its impact on cellular physiological functions. Therefore, a microinjection device is required.

[0003] Existing single-cell microinjection devices load the sample to be injected into the injection needle, place the culture dish containing the cells on the microscope stage to position the cells, install and position the injection needle at the same time, and then slowly insert the injection needle into the cell to accurately inject the sample into the target location within the cell. After the injection is completed, the injection needle is withdrawn and the cells are returned to the culture environment to observe the subsequent reaction.

[0004] However, the aforementioned microinjection device still has some problems. In practical applications, the syringe angle cannot be arbitrarily adjusted. When faced with single cells of different shapes, sizes, and locations, the syringe cannot penetrate the cells at the optimal angle. Some cells grow on the edge of the culture dish or at an angle, increasing the risk of cell rupture. Therefore, a single-cell microinjection device is proposed to address these problems. Summary of the Invention

[0005] To overcome the shortcomings of existing technologies and solve the problems mentioned in the background art, this utility model proposes a single-cell microinjection device.

[0006] The technical solution adopted by this utility model to solve its technical problem is as follows: A single-cell microinjection device of this utility model includes a worktable, a support frame installed directly above the worktable, a rotating shaft rotatably mounted through one side of the support frame, a mounting bracket installed at one end of the rotating shaft, rotating shafts rotatably mounted through both sides of the mounting bracket, a syringe assembled between the two sides of the rotating shaft, a side plate assembled on one side of the rotating shaft, a locking screw rotatably mounted inside one end of the side plate, a gear groove installed at the other end of the rotating shaft, a support plate mounted on one side of the top of the support frame, a limiting rod slidably mounted through the support plate, a locking block adapted to the gear groove at the bottom end of the limiting rod, a limiting block installed at the top end of the limiting rod, and a tension spring fixedly connected between the limiting block and the support plate. The tension spring is sleeved on the outside of the limiting rod. Through the cooperation of the rotating shaft and the rotating shaft, the angle of the syringe can be arbitrarily adjusted, allowing the syringe to more flexibly aim at the target cell during microinjection and improving the injection effect.

[0007] Preferably, the top side of the workbench has an upward-opening slide rail, a sliding block is slidably installed inside the slide rail, a fixed frame is installed on the top side of the sliding block, a fastening screw is rotatably installed inside one end of the sliding block, a rectangular groove is opened inside the top side of the fixed frame, a movable block is slidably installed inside the rectangular groove, a cylinder is installed on one side of the top of the fixed frame, the working end of the cylinder is fixedly connected to one side of the movable block, a hydraulic cylinder is installed on the top side of the movable block, and the support frame is assembled on the bottom side of the working end of the hydraulic cylinder. Through the sliding of the sliding block on the slide rail and the coordinated action of the cylinder and the hydraulic cylinder, the position of the syringe can be precisely adjusted to ensure that the syringe can be aimed at the single cell to be injected, thereby improving injection accuracy and work efficiency.

[0008] Preferably, the worktable has an internal mounting cavity, through which a connecting shaft is rotatably mounted. A disc is mounted on the outer side of the middle end of the connecting shaft. The disc has four staggered arc-shaped grooves and U-shaped grooves. A servo motor is mounted inside the bottom of the mounting cavity. A rotating disk that matches the arc-shaped groove is mounted on the output end of the servo motor. A connecting rod is mounted on the outer side of the rotating disk. A protruding rod that matches the U-shaped groove is mounted on the top side of one end of the connecting rod. The servo motor drives the rotating disk to rotate in the arc-shaped groove, which in turn drives the connecting rod and the protruding rod to move in the U-shaped groove, realizing intermittent rotation of the disc. This allows the device to perform injection operations continuously, improving the injection efficiency of single cells.

[0009] Preferably, a support plate is installed on the top side of the connecting shaft, and four placement slots are equidistantly installed on the top side of the support plate for placing culture dishes.

[0010] Preferably, two sliding grooves are symmetrically formed on the bottom side of each placement groove. A support rod is fixedly connected between the two sides of each sliding groove. A slider is slidably installed on the outer side of one end of each support rod inside the sliding groove. An arc-shaped clamp is installed on the top side of each slider. A spring is fixedly connected between each sliding groove and the slider. The spring is sleeved on the outer side of the support rod. The arc-shaped clamp in each placement groove is symmetrically arranged, which can realize the limiting and fixing of the culture dish containing the cells to be injected, so as to ensure the stability of the culture dish during the injection process and avoid damage to the cells due to shaking.

[0011] Preferably, a control panel is installed on one side of the workbench. The control panel is used to operate and control the electrical components inside the device, thereby realizing centralized control of the electrical components in the device.

[0012] The advantages of this utility model are:

[0013] 1. This utility model involves lifting the limiting block upwards, causing the limiting rod to move upwards, stretching the tension spring, and disengaging the locking block from the gear groove. Then, rotating the rotating shaft drives the mounting bracket to rotate, thereby changing the angle of the syringe in the X-axis direction and adjusting the angle of the syringe in the Y-axis direction. Loosening the locking screw releases the fixation of the rotating shaft, and rotating the syringe through the rotation of the rotating shaft allows for adjustment of the syringe angle. This enables arbitrary adjustment of the syringe angle, allowing the syringe with the injection sample to more flexibly align with the target cells during microinjection, thus improving the injection effect.

[0014] 2. The output end of the servo motor of this utility model drives the rotating disk to rotate, causing the connecting rod to move the protruding rod in the U-shaped groove. As the rotating disk rotates, when the protruding rod slides out of the U-shaped groove, it drives the connecting shaft and the support disk to move. When the rotating disk moves in the arc-shaped groove, it will temporarily stop the disk from continuing to rotate. At this time, the disk is in a stationary state, thus realizing the intermittent movement of the culture dish. The device can perform injection operations continuously, improving the injection efficiency of single cells. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of the intermediate axis side view of the present invention;

[0017] Figure 2 This is a schematic diagram of the main structure of a single-cell microinjection device.

[0018] Figure 3 This is a schematic diagram of the syringe angle adjustment component.

[0019] Figure 4 This is a schematic diagram of the intermittent motion component structure;

[0020] Figure 5 This is a schematic diagram of the structure of the petri dish fixation assembly.

[0021] In the diagram: 1. Workbench; 2. Support frame; 3. Rotating shaft; 4. Mounting frame; 5. Rotating shaft; 6. Syringe; 7. Side plate; 8. Locking screw; 9. Gear groove; 10. Support plate; 11. Limiting rod; 12. Clamping block; 13. Limiting block; 14. Tension spring; 15. Slide rail; 16. Sliding block; 17. Fixing frame; 18. Fastening screw; 19. Rectangular groove; 20. Movable block; 21. Cylinder; 22. Hydraulic cylinder; 23. Connecting shaft; 24. Disc; 25. Arc groove; 26. U-shaped groove; 27. Servo motor; 28. Rotating disk; 29. ​​Connecting rod; 30. Protruding rod; 31. Support plate; 32. Placement groove; 33. Slide groove; 34. Support rod; 35. Slider; 36. Arc clamping plate; 37. Spring 1; 38. Control panel. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0023] Please see Figure 1-3 As shown, a single-cell microinjection device includes a worktable 1, a support frame 2 mounted on the top of the worktable 1, a rotating shaft 3 rotatably mounted through one side of the support frame 2, a mounting frame 4 mounted at one end of the rotating shaft 3, rotating shafts 5 rotatably mounted through both sides of the mounting frame 4, a syringe 6 assembled between the two sides of the rotating shaft 5, a side plate 7 assembled on one side of the rotating shaft 5, a locking screw 8 rotatably mounted inside one end of the side plate 7, a gear groove 9 mounted at the other end of the rotating shaft 3, a support plate 10 mounted on one side of the top of the support frame 2, a limiting rod 11 slidably mounted through the inside of the support plate 10, a locking block 12 adapted to the gear groove 9 assembled at the bottom end of the limiting rod 11, a limiting block 13 mounted at the top end of the limiting rod 11, a tension spring 14 fixedly connected between the limiting block 13 and the support plate 10, and the tension spring 14 sleeved on the outside of the limiting rod 11;

[0024] The workbench 1 has an upward-opening slide rail 15 inside its top side. A sliding block 16 is slidably installed inside the slide rail 15. A fixing frame 17 is installed on the top side of the sliding block 16. A fastening screw 18 is rotatably installed inside one end of the sliding block 16. A rectangular groove 19 is opened inside the top side of the fixing frame 17. A movable block 20 is slidably installed inside the rectangular groove 19. A cylinder 21 is installed on one side of the top of the fixing frame 17. The working end of the cylinder 21 is fixedly connected to one side of the movable block 20. A hydraulic cylinder 22 is installed on the top side of the movable block 20. A support frame 2 is assembled on the bottom side of the working end of the hydraulic cylinder 22. A control panel 38 is installed on one side of the workbench 1. During operation, in practical applications, the angle of the syringe 6 cannot be arbitrarily adjusted. When facing single cells of different shapes, sizes, and positions, the syringe 6 cannot penetrate the cells at the optimal angle. Some cells grow at the edge of the culture dish or at an inclined angle, increasing the risk of cell death. To mitigate the risk of cell rupture, when adjusting the angle of syringe 6, first pull up the limiting block 13. The limiting rod 11 moves upward, stretching the tension spring 14 and disengaging the locking block 12 from the gear groove 9. Then, rotate the rotating shaft 3, causing the mounting bracket 4 to rotate, thereby changing the angle of syringe 6 in the X-axis direction. After adjustment, release the limiting rod 11. Under the elastic force of the tension spring 14, the limiting rod 11 moves downward, and the locking block 12 re-engages in the gear groove 9, thus fixing the angle of syringe 6. To adjust the angle of syringe 6 in the Y-axis direction, loosen the locking screw 8 to release the fixation of the rotating shaft 5. Rotate the rotating shaft 5 to rotate syringe 6, thereby adjusting the angle of syringe 6. After adjustment, tighten the rotating shaft 5 with the locking screw 8 to lock the angle, allowing arbitrary adjustment of the syringe 6 angle. This enables syringe 6 with the injection sample to more flexibly align with the target cells during microinjection, improving the injection effect.

[0025] To adjust the position of syringe 6 in the X-axis direction, loosen the fastening screw 18, and slide the sliding block 16 in the slide rail 15. After sliding to the appropriate position as needed, tighten the fastening screw 18 to fix the sliding block 16 on the slide rail 15. At this time, the fixing bracket 17 moves together with the sliding block 16, thereby initially adjusting the position of syringe 6 in the X-axis direction.

[0026] The cylinder 21 is activated via the control panel 38. The actuating end of the cylinder 21 pushes the movable block 20 to slide within the rectangular groove 19, thereby driving the hydraulic cylinder 22 and the support frame 2 to move together in the Y-axis direction. This allows for precise fine-tuning of the position of the syringe 6 in the Y-axis direction. During the injection operation, the actuating end of the hydraulic cylinder 22 extends and retracts vertically, which can drive the support frame 2 and the syringe 6 with the injection sample to move vertically. This allows the syringe 6 to gradually approach the culture dish to inject the cells, achieving precise adjustment of the vertical position of the syringe 6 and ensuring that the syringe 6 can accurately aim at the single cell to be injected.

[0027] Please see Figure 1 , 2 As shown in Figures 4 and 5, the workbench 1 has an internal mounting cavity. A connecting shaft 23 is rotatably mounted inside the mounting cavity. A disc 24 is mounted on the outer side of the middle end of the connecting shaft 23. The disc 24 has four interlaced arc-shaped grooves 25 and U-shaped grooves 26. A servo motor 27 is mounted inside the bottom side of the mounting cavity. A rotating disk 28 that matches the arc-shaped grooves 25 is mounted on the output end of the servo motor 27. A connecting rod 29 is mounted on the outer side of the rotating disk 28. A protruding rod 30 that matches the U-shaped groove 26 is mounted on the top side of one end of the connecting rod 29.

[0028] A support plate 31 is installed on the top side of the connecting shaft 23, and four placement slots 32 are equidistantly installed on the top side of the support plate 31.

[0029] Each placement slot 32 has two symmetrically formed sliding grooves 33 on its bottom side. A support rod 34 is fixedly connected between the two sides of each sliding groove 33. A slider 35 is slidably mounted on the outer side of one end of each support rod 34 inside the sliding groove 33. An arc-shaped clamp 36 is mounted on the top side of each slider 35. A spring 37 is fixedly connected between each sliding groove 33 and the slider 35, and the spring 37 is sleeved on the outer side of the support rod 34. The arc-shaped clamp 36 in each placement slot 32 is symmetrically arranged. During operation, in single-cell biology research within plant cells, specific gene fragments can be introduced into single cells via microinjection. To observe gene expression in single cells and its effects on cellular physiological functions, a microinjection device is required. A culture dish containing cells to be injected is placed in a placement groove 32. During placement, the compression of the culture dish causes the slider 35 to slide along the support rod 34 in a groove 33. The spring 37 is stretched, and the elastic force generated by the spring 37 causes two symmetrical arc-shaped clamps 36 to tightly clamp the culture dish, thereby achieving the limitation and fixation of the culture dish. During the injection process, the culture dish will not shake due to the vibration of the device, ensuring the stability of the cells during the injection process and avoiding damage to the cells due to shaking.

[0030] Then, the servo motor 27 is started. The output of the servo motor 27 drives the rotating disk 28 to rotate, which causes the connecting rod 29 to move the protruding rod 30 within the U-shaped groove 26. As the rotating disk 28 rotates, when the protruding rod 30 slides out of the U-shaped groove 26, it drives the connecting shaft 23 and the support disk 31 to move. When the rotating disk 28 moves within the arc-shaped groove 25, it temporarily prevents the disc 24 from continuing to rotate. At this time, the disc 24 is in a stationary state, thus realizing the intermittent movement of the culture dish. When the culture dish in one placement groove 32 has been injected, the support disk 31 rotates, rotating the next placement groove 32 to the injection position, enabling the device to perform continuous injection operations and improving the injection efficiency of single cells.

[0031] Working principle: The culture dish containing the cells to be injected is placed in the placement groove 32. During placement, the compression of the culture dish causes the slider 35 to slide along the support rod 34 in the groove 33. The spring 37 is stretched, and the elastic force generated by the spring 37 causes the two symmetrical arc-shaped clamps 36 to tightly clamp the culture dish, thereby achieving the limitation and fixation of the culture dish. During the injection process, the culture dish will not shake due to the vibration of the device, ensuring the stability of the cells during the injection process and avoiding damage to the cells due to shaking.

[0032] Then, the servo motor 27 is started. The output end of the servo motor 27 drives the rotating disk 28 to rotate, so that the connecting rod 29 drives the protruding rod 30 to move in the U-shaped groove 26. As the rotating disk 28 rotates, when the protruding rod 30 slides out of the U-shaped groove 26, it drives the connecting shaft 23 and the support disk 31 to move. When the rotating disk 28 moves in the arc groove 25, it will temporarily stop the disk 24 from continuing to rotate. At this time, the disk 24 is in a stationary state, thus realizing the intermittent movement of the culture dish. When the culture dish in one placement groove 32 is injected, the support disk 31 rotates to rotate the next placement groove 32 to the injection position, so that the device can perform injection operations continuously and improve the injection efficiency of single cells.

[0033] When the angle of syringe 6 needs to be adjusted, first pull up the limiting block 13, the limiting rod 11 moves upward, the tension spring 14 is stretched, and the locking block 12 disengages from the gear groove 9. Then rotate the rotating shaft 3 to drive the mounting bracket 4 to rotate, thereby changing the angle of syringe 6 in the X-axis direction. After adjustment, release the limiting rod 11. Under the elastic force of the tension spring 14, the limiting rod 11 moves downward, and the locking block 12 re-engages in the gear groove 9, thus fixing the angle of syringe 6. To adjust the angle of syringe 6 in the Y-axis direction, loosen the locking screw 8 to release the fixation of the rotating shaft 5. The rotation of the rotating shaft 5 causes syringe 6 to rotate, thereby adjusting the angle of syringe 6. After adjustment, tighten the rotating shaft 5 with the locking screw 8 to lock the angle, enabling arbitrary adjustment of the angle of syringe 6. This allows syringe 6 with the injection sample to be more flexibly aligned with the target cells during microinjection, improving the injection effect.

[0034] To adjust the position of syringe 6 in the X-axis direction, loosen the fastening screw 18, and slide the sliding block 16 in the slide rail 15. After sliding to the appropriate position as needed, tighten the fastening screw 18 to fix the sliding block 16 on the slide rail 15. At this time, the fixing bracket 17 moves together with the sliding block 16, thereby initially adjusting the position of syringe 6 in the X-axis direction.

[0035] The cylinder 21 is activated via the control panel 38. The actuating end of the cylinder 21 pushes the movable block 20 to slide within the rectangular groove 19, thereby driving the hydraulic cylinder 22 and the support frame 2 to move together in the Y-axis direction. This allows for precise fine-tuning of the position of the syringe 6 in the Y-axis direction. During the injection operation, the actuating end of the hydraulic cylinder 22 extends and retracts vertically, which can drive the support frame 2 and the syringe 6 with the injection sample to move vertically. This allows the syringe 6 to gradually approach the culture dish to inject the cells, achieving precise adjustment of the vertical position of the syringe 6 and ensuring that the syringe 6 can accurately aim at the single cell to be injected.

[0036] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0037] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model.

Claims

1. A single-cell microinjection device, characterized in that: The system includes a workbench (1), a support frame (2) mounted directly above the workbench (1), a rotating shaft (3) rotatably mounted through one side of the support frame (2), a mounting bracket (4) mounted at one end of the rotating shaft (3), rotating shafts (5) rotatably mounted through both sides of the mounting bracket (4), a syringe (6) assembled between the two sides of the rotating shaft (5), a side plate (7) assembled on one side of the rotating shaft (5), and a locking screw (8) rotatably mounted inside one end of the side plate (7). The other end is equipped with a gear groove (9), and a support plate (10) is installed on one side of the top of the support frame (2). A limit rod (11) is slidably installed inside the support plate (10). A locking block (12) that matches the gear groove (9) is fitted at the bottom of the limit rod (11). A limit block (13) is installed at the top of the limit rod (11). A tension spring (14) is fixed between the limit block (13) and the support plate (10). The tension spring (14) is sleeved on the outside of the limit rod (11).

2. The single-cell microinjection device according to claim 1, characterized in that: The workbench (1) has an upward-opening slide rail (15) inside its top side. A sliding block (16) is slidably installed inside the slide rail (15). A fixed frame (17) is installed on the top side of the sliding block (16). A fastening screw (18) is rotatably installed inside one end of the sliding block (16). A rectangular groove (19) is opened inside the top side of the fixed frame (17). A movable block (20) is slidably installed inside the rectangular groove (19). A cylinder (21) is installed on one side of the top of the fixed frame (17). The working end of the cylinder (21) is fixedly connected to one side of the movable block (20). A hydraulic cylinder (22) is installed on the top side of the movable block (20). The support frame (2) is assembled on the bottom side of the working end of the hydraulic cylinder (22).

3. The single-cell microinjection device according to claim 2, characterized in that: The workbench (1) has an installation cavity inside, and a connecting shaft (23) is rotatably installed inside the installation cavity. A disc (24) is installed on the outer side of the middle end of the connecting shaft (23). The disc (24) has four arc-shaped grooves (25) and U-shaped grooves (26) staggered inside. A servo motor (27) is installed inside the bottom side of the installation cavity. A rotating disk (28) that matches the arc-shaped groove (25) is installed at the output end of the servo motor (27). A connecting rod (29) is installed on the outer side of the rotating disk (28). A protruding rod (30) that matches the U-shaped groove (26) is installed on the top side of one end of the connecting rod (29).

4. The single-cell microinjection device according to claim 3, characterized in that: A support plate (31) is installed on the top side of the connecting shaft (23), and four placement slots (32) are installed equidistantly on the top side of the support plate (31).

5. A single-cell microinjection device according to claim 4, characterized in that: Two sliding grooves (33) are symmetrically opened on the bottom side of each placement groove (32). A support rod (34) is fixed between the two sides of each sliding groove (33). A slider (35) is slidably installed on the outer side of one end of the support rod (34) inside the sliding groove (33). An arc-shaped clamp (36) is installed on the top side of each slider (35). A spring (37) is fixed between the sliding groove (33) and the slider (35). The spring (37) is sleeved on the outer side of the support rod (34). The arc-shaped clamp (36) in each placement groove (32) is symmetrically arranged.

6. The single-cell microinjection device according to claim 1, characterized in that: A control panel (38) is installed on one side of the workbench (1), and the control panel (38) is used to control the operation of the electrical components inside the device.

Images